TY  - JOUR
AU  - Radovanović, Marina
AU  - Lazarević, Nevena
AU  - Novaković, Jovana
AU  - Jeremić, Nevena
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Bradić, Jovana
AU  - Pecarski, Danijela
AU  - Tel-Çayan, Gülsen
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Gregori, Andrej
AU  - Petrović, Jovana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5792
AB  - The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome
IS  - 6
VL  - 16
DO  - 10.3390/ph16060787
SP  - 787
ER  - 

@article{
author = "Radovanović, Marina and Lazarević, Nevena and Novaković, Jovana and Jeremić, Nevena and Jakovljević, Vladimir and Živković, Vladimir and Bradić, Jovana and Pecarski, Danijela and Tel-Çayan, Gülsen and Glamočlija, Jasmina and Soković, Marina and Gregori, Andrej and Petrović, Jovana",
year = "2023",
abstract = "The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome",
number = "6",
volume = "16",
doi = "10.3390/ph16060787",
pages = "787"
}

Radovanović, M., Lazarević, N., Novaković, J., Jeremić, N., Jakovljević, V., Živković, V., Bradić, J., Pecarski, D., Tel-Çayan, G., Glamočlija, J., Soković, M., Gregori, A.,& Petrović, J.. (2023). Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals
Basel: MDPI., 16(6), 787.
https://doi.org/10.3390/ph16060787

Radovanović M, Lazarević N, Novaković J, Jeremić N, Jakovljević V, Živković V, Bradić J, Pecarski D, Tel-Çayan G, Glamočlija J, Soković M, Gregori A, Petrović J. Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals. 2023;16(6):787.
doi:10.3390/ph16060787 .

Radovanović, Marina, Lazarević, Nevena, Novaković, Jovana, Jeremić, Nevena, Jakovljević, Vladimir, Živković, Vladimir, Bradić, Jovana, Pecarski, Danijela, Tel-Çayan, Gülsen, Glamočlija, Jasmina, Soković, Marina, Gregori, Andrej, Petrović, Jovana, "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome" in Pharmaceuticals, 16, no. 6 (2023):787,
https://doi.org/10.3390/ph16060787 . .

TY  - JOUR
AU  - Rakić, Dejana
AU  - Joksimović Jović, Jovana
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Sretenović, Jasmina
AU  - Nikolić, Marina
AU  - Jović, Nikola
AU  - Bićanin Ilić, Marija
AU  - Arsenijević, Petar
AU  - Dimitrijević, Aleksandra
AU  - Vulović, Tatjana
AU  - Ristić, Nataša
AU  - Bulatović, Kristina
AU  - Bolevich, Sergej
AU  - Stijak, Lazar
AU  - Pantović, Suzana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6087
AB  - Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.
PB  - Basel: MDPI
T2  - Medicina
T1  - High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats
IS  - 6
VL  - 59
DO  - 10.3390/medicina59061104
SP  - 1104
ER  - 

@article{
author = "Rakić, Dejana and Joksimović Jović, Jovana and Jakovljević, Vladimir and Živković, Vladimir and Nikolić, Maja and Sretenović, Jasmina and Nikolić, Marina and Jović, Nikola and Bićanin Ilić, Marija and Arsenijević, Petar and Dimitrijević, Aleksandra and Vulović, Tatjana and Ristić, Nataša and Bulatović, Kristina and Bolevich, Sergej and Stijak, Lazar and Pantović, Suzana",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.",
publisher = "Basel: MDPI",
journal = "Medicina",
title = "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats",
number = "6",
volume = "59",
doi = "10.3390/medicina59061104",
pages = "1104"
}

Rakić, D., Joksimović Jović, J., Jakovljević, V., Živković, V., Nikolić, M., Sretenović, J., Nikolić, M., Jović, N., Bićanin Ilić, M., Arsenijević, P., Dimitrijević, A., Vulović, T., Ristić, N., Bulatović, K., Bolevich, S., Stijak, L.,& Pantović, S.. (2023). High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina
Basel: MDPI., 59(6), 1104.
https://doi.org/10.3390/medicina59061104

Rakić D, Joksimović Jović J, Jakovljević V, Živković V, Nikolić M, Sretenović J, Nikolić M, Jović N, Bićanin Ilić M, Arsenijević P, Dimitrijević A, Vulović T, Ristić N, Bulatović K, Bolevich S, Stijak L, Pantović S. High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina. 2023;59(6):1104.
doi:10.3390/medicina59061104 .

Rakić, Dejana, Joksimović Jović, Jovana, Jakovljević, Vladimir, Živković, Vladimir, Nikolić, Maja, Sretenović, Jasmina, Nikolić, Marina, Jović, Nikola, Bićanin Ilić, Marija, Arsenijević, Petar, Dimitrijević, Aleksandra, Vulović, Tatjana, Ristić, Nataša, Bulatović, Kristina, Bolevich, Sergej, Stijak, Lazar, Pantović, Suzana, "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats" in Medicina, 59, no. 6 (2023):1104,
https://doi.org/10.3390/medicina59061104 . .

TY  - CONF
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
AU  - Milosavljević, Zoran
AU  - Jakovljević, Vladimir
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6274
AB  - The study included 32 Wistar albino male rats, 10 weeks old, divided into 4 groups: control (T-N-), nandrolone (T-N+), swimming (T+N-) and swimming plus nandrolone (T+N+) groups. The T+ positive groups swam for 4 weeks, 1 hour/day, 5 days/week. The N+ positive groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Five micrometer thick sections were immunohistochemically stained, while LH and FSH cells were exposed to the morphometric analysis. Circulating level of LH, FSH and testosterone were measured. Body weight did not significantly decrease in all experimental groups compared to control. Absolute pituitary weights were decreased in T-N+ and increased in T+ positive groups. Relative pituitary weights were increased in all experimental groups compared to control. Volume density of LH cells was decreased for 48% in T-N+, for 22% in T+N- and for 35% in T+N+ group. Number of LH cells on mm2 was decreased for 36% in T-N+, for 3% in T+N- and for 27% in T+N+ group. Volume densities of FSH cells were decreased for 39% in T-N+, for 5.5% in T+N- and for 30% in T+N+ compared to control. Number of FSH cells was decreased for 13% in T-N+, for 1% and 15% in T+N+ group compared to control. Serum levels of LH were decreased in N+ positive groups, while FSH level was increased in T+N- group. Serum level of testosterone was increased in all experimental groups compared to control value. Nandrolone alone or combined with swimming decreased morphometric parameters of gonadotropic cells.
PB  - Belgrade: Serbian Physiology Society
C3  - Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia
T1  - Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6274
ER  - 

@conference{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Milošević, Verica and Milosavljević, Zoran and Jakovljević, Vladimir",
year = "2019",
abstract = "The study included 32 Wistar albino male rats, 10 weeks old, divided into 4 groups: control (T-N-), nandrolone (T-N+), swimming (T+N-) and swimming plus nandrolone (T+N+) groups. The T+ positive groups swam for 4 weeks, 1 hour/day, 5 days/week. The N+ positive groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Five micrometer thick sections were immunohistochemically stained, while LH and FSH cells were exposed to the morphometric analysis. Circulating level of LH, FSH and testosterone were measured. Body weight did not significantly decrease in all experimental groups compared to control. Absolute pituitary weights were decreased in T-N+ and increased in T+ positive groups. Relative pituitary weights were increased in all experimental groups compared to control. Volume density of LH cells was decreased for 48% in T-N+, for 22% in T+N- and for 35% in T+N+ group. Number of LH cells on mm2 was decreased for 36% in T-N+, for 3% in T+N- and for 27% in T+N+ group. Volume densities of FSH cells were decreased for 39% in T-N+, for 5.5% in T+N- and for 30% in T+N+ compared to control. Number of FSH cells was decreased for 13% in T-N+, for 1% and 15% in T+N+ group compared to control. Serum levels of LH were decreased in N+ positive groups, while FSH level was increased in T+N- group. Serum level of testosterone was increased in all experimental groups compared to control value. Nandrolone alone or combined with swimming decreased morphometric parameters of gonadotropic cells.",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia",
title = "Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6274"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Milošević, V., Milosavljević, Z.,& Jakovljević, V.. (2019). Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats. in Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia
Belgrade: Serbian Physiology Society., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_6274

Sretenović J, Živković V, Srejović I, Ajdžanović V, Milošević V, Milosavljević Z, Jakovljević V. Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats. in Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia. 2019;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_6274 .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Milošević, Verica, Milosavljević, Zoran, Jakovljević, Vladimir, "Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats" in Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia (2019):32,
https://hdl.handle.net/21.15107/rcub_ibiss_6274 .

TY  - CONF
AU  - Sretenović, Jasmina
AU  - Jakovljević, Biljana
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
AU  - Jakovljević, Vladimir
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6058
AB  - The aim of this study was to show the effects of moderate-intensity and high-intensity training on morfometric parameters of the left ventricle miocardium in normotensive and hypertensive rats. The study included 32-male Wistar albino and spontaneously hypertensive rats, divided into 6 groups: control normotensive (CTRL), normotensive moderate-intensity training (MIT), normotensive high-intensity training (HIIT), control hypertensive (SHR), hypertensive moderate-The rat ton Mist Roups and by peaterive high since sit training (Si HI days/week, Ih/day, while HIIT groups ran on speed from 35 to 55m/min, 30s in 5 cycles, with a 3 min period of rest between the cycles. After 4-weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin. Five micrometer thick sections were H&E stained. Captured microscopic images were processed by special software for image analysis to quantify the results. Body weights were decreased in all groups of hypertensive rats compared to normotensive rats. Relative heart weights were increased in hypertensive rats compared to normotensive rats. Longitudinal section diameter of cardiac muscle cells was decreased in SHR-MIT group for 13% compared to MIT, while in SHR-HIIT group the same parameter decreased for 17% compared to HIIT. Cross section muscle cell area was decreased: in SHR fl 7% compared to CTRL, in SHR-MIT for 6% compared to MIT, and in SHR-HIIT for 9% in comparison with HIIT. Both types of training caused significant hypertrophy of the left ventricle myocardium in normotensive and hypertensive rats
PB  - Kragujevac: University of Kragujevac, Faculty of Medical Sciences
C3  - Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia
T1  - Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6058
ER  - 

@conference{
author = "Sretenović, Jasmina and Jakovljević, Biljana and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Milošević, Verica and Jakovljević, Vladimir",
year = "2018",
abstract = "The aim of this study was to show the effects of moderate-intensity and high-intensity training on morfometric parameters of the left ventricle miocardium in normotensive and hypertensive rats. The study included 32-male Wistar albino and spontaneously hypertensive rats, divided into 6 groups: control normotensive (CTRL), normotensive moderate-intensity training (MIT), normotensive high-intensity training (HIIT), control hypertensive (SHR), hypertensive moderate-The rat ton Mist Roups and by peaterive high since sit training (Si HI days/week, Ih/day, while HIIT groups ran on speed from 35 to 55m/min, 30s in 5 cycles, with a 3 min period of rest between the cycles. After 4-weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin. Five micrometer thick sections were H&E stained. Captured microscopic images were processed by special software for image analysis to quantify the results. Body weights were decreased in all groups of hypertensive rats compared to normotensive rats. Relative heart weights were increased in hypertensive rats compared to normotensive rats. Longitudinal section diameter of cardiac muscle cells was decreased in SHR-MIT group for 13% compared to MIT, while in SHR-HIIT group the same parameter decreased for 17% compared to HIIT. Cross section muscle cell area was decreased: in SHR fl 7% compared to CTRL, in SHR-MIT for 6% compared to MIT, and in SHR-HIIT for 9% in comparison with HIIT. Both types of training caused significant hypertrophy of the left ventricle myocardium in normotensive and hypertensive rats",
publisher = "Kragujevac: University of Kragujevac, Faculty of Medical Sciences",
journal = "Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia",
title = "Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6058"
}

Sretenović, J., Jakovljević, B., Živković, V., Srejović, I., Ajdžanović, V., Milošević, V.,& Jakovljević, V.. (2018). Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats. in Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia
Kragujevac: University of Kragujevac, Faculty of Medical Sciences., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_6058

Sretenović J, Jakovljević B, Živković V, Srejović I, Ajdžanović V, Milošević V, Jakovljević V. Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats. in Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia. 2018;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_6058 .

Sretenović, Jasmina, Jakovljević, Biljana, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Milošević, Verica, Jakovljević, Vladimir, "Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats" in Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia (2018):32,
https://hdl.handle.net/21.15107/rcub_ibiss_6058 .

TY  - CONF
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
AU  - Jakovljević, Vladimir
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5993
AB  - Anabolic androgenic steroids (ASs) are synthetic analogs of testosterone. Because of its anabolic effect, in recent decades, AAS became very popular among professional and recreational sports man. The aim of this study was to examine the chronic effects of exercise training alone or combined with supraphysiological doses of nandrolone decanoate (ND) on cardiac muscle of the left ventricle. The study included 32 Wistar albino male rats, divided into 4 groups: control (I-N-), nandrolone (I-N+), exercise training (T+N-) and exercise training plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 3 weeks, 1 hour/day, 5 days/week. The T-N+ and T+N+ groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. After 3 weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin.
Five micrometer thick sections were stained with hematoxylin and eosin (H/E). Captured microscopic images were processed by special software for image analysis to quantify results. Our result show that nandrolone alone causes left ventricular wall thickening for 4% (p > 0.05), training alone 14% (p > 0.05) while training combined with nandrolone leads to increased wall thickening for 24% (p < 0.05). Longitudinal section diameter of cardiac muscle cells was increased for 6% (p >0.05), in T-N+ for 23% (p < 0.05), in T*N- and for 33% (p < 0.05) in T+N+ group. Comparison between the experimental groups showed that the largest difference of longitudinal section diameter can be observed between T-N+ and T+N+ (24%; p < 0.05) and the lowest difference was found between T+N- and T#N+ (8%; p > 0.05). Cross section muscle cell area was increased in T-N+ for 7% (p > 0.05), in T+N- for 29% (p < 0.05), in T+N+ for 31% (p < 0.05). Comparison between the experimental groups showed that the difference of cross section area between T-N+ and T+N+ was 22% (p < 0.05) and the lowest, non-significant difference was found between T+N- and T+N+ (1.6%; p > 0.05). Chronic administration of supraphysiological doses of ND alone promote mild cardiac muscle hypertrophy, but only in combination with the exercise training the degree of the cardiac muscle hypertrophy of the left ventricle is significant.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5993
ER  - 

@conference{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Milošević, Verica and Jakovljević, Vladimir",
year = "2018",
abstract = "Anabolic androgenic steroids (ASs) are synthetic analogs of testosterone. Because of its anabolic effect, in recent decades, AAS became very popular among professional and recreational sports man. The aim of this study was to examine the chronic effects of exercise training alone or combined with supraphysiological doses of nandrolone decanoate (ND) on cardiac muscle of the left ventricle. The study included 32 Wistar albino male rats, divided into 4 groups: control (I-N-), nandrolone (I-N+), exercise training (T+N-) and exercise training plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 3 weeks, 1 hour/day, 5 days/week. The T-N+ and T+N+ groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. After 3 weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin.
Five micrometer thick sections were stained with hematoxylin and eosin (H/E). Captured microscopic images were processed by special software for image analysis to quantify results. Our result show that nandrolone alone causes left ventricular wall thickening for 4% (p > 0.05), training alone 14% (p > 0.05) while training combined with nandrolone leads to increased wall thickening for 24% (p < 0.05). Longitudinal section diameter of cardiac muscle cells was increased for 6% (p >0.05), in T-N+ for 23% (p < 0.05), in T*N- and for 33% (p < 0.05) in T+N+ group. Comparison between the experimental groups showed that the largest difference of longitudinal section diameter can be observed between T-N+ and T+N+ (24%; p < 0.05) and the lowest difference was found between T+N- and T#N+ (8%; p > 0.05). Cross section muscle cell area was increased in T-N+ for 7% (p > 0.05), in T+N- for 29% (p < 0.05), in T+N+ for 31% (p < 0.05). Comparison between the experimental groups showed that the difference of cross section area between T-N+ and T+N+ was 22% (p < 0.05) and the lowest, non-significant difference was found between T+N- and T+N+ (1.6%; p > 0.05). Chronic administration of supraphysiological doses of ND alone promote mild cardiac muscle hypertrophy, but only in combination with the exercise training the degree of the cardiac muscle hypertrophy of the left ventricle is significant.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats",
pages = "150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5993"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Milošević, V.,& Jakovljević, V.. (2018). Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 150.
https://hdl.handle.net/21.15107/rcub_ibiss_5993

Sretenović J, Živković V, Srejović I, Ajdžanović V, Milošević V, Jakovljević V. Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:150.
https://hdl.handle.net/21.15107/rcub_ibiss_5993 .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Milošević, Verica, Jakovljević, Vladimir, "Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):150,
https://hdl.handle.net/21.15107/rcub_ibiss_5993 .