TY  - JOUR
AU  - Dragić, Milorad
AU  - Zeljković, Milica
AU  - Stevanović, Ivana
AU  - Ilić, Tihomir
AU  - Ilić, Nela
AU  - Nedeljković, Nadezda
AU  - Ninković, Milica
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32599126
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3768
AB  - Multiple sclerosis (MS) is a chronic neurodegenerative disease caused by inflammatory processes in the central nervous system (CNS). Decades of research led to discovery of several disease-modifying therapeutics strategies with moderate success. Experimental autoimmune encephalomyelitis (EAE) is currently the most commonly used experimental model for MS and for studying various therapeutic approaches. Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neurostimulation technique with multiple beneficial effects on healthy as well as CNS with pathology. However, the molecular and cellular mechanisms of rTMS on acute EAE are scarce. Our study demonstrated beneficial effects of theta-burst stimulation (TBS), an experimental paradigm of rTMS, on disease course of acute EAE. TBS treatment attenuated reactive gliosis, restored myelin sheet and down-regulated expression of vimentin in EAE rats. These effects were reflected through reduced clinical parameters, shorter duration of illness and days spent in paralysis. Based on our research, rTMS deserves further considerations for its neuroprotective effect on EAE, and is an excellent candidate for further research and points that it could be used for more than for simple symptomatic therapy.
PB  - Elsevier BV
T2  - Brain Research Bulletin
T1  - Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.
VL  - 162
DO  - 10.1016/j.brainresbull.2020.06.013
SP  - 208
EP  - 217
ER  - 

@article{
author = "Dragić, Milorad and Zeljković, Milica and Stevanović, Ivana and Ilić, Tihomir and Ilić, Nela and Nedeljković, Nadezda and Ninković, Milica",
year = "2020",
abstract = "Multiple sclerosis (MS) is a chronic neurodegenerative disease caused by inflammatory processes in the central nervous system (CNS). Decades of research led to discovery of several disease-modifying therapeutics strategies with moderate success. Experimental autoimmune encephalomyelitis (EAE) is currently the most commonly used experimental model for MS and for studying various therapeutic approaches. Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neurostimulation technique with multiple beneficial effects on healthy as well as CNS with pathology. However, the molecular and cellular mechanisms of rTMS on acute EAE are scarce. Our study demonstrated beneficial effects of theta-burst stimulation (TBS), an experimental paradigm of rTMS, on disease course of acute EAE. TBS treatment attenuated reactive gliosis, restored myelin sheet and down-regulated expression of vimentin in EAE rats. These effects were reflected through reduced clinical parameters, shorter duration of illness and days spent in paralysis. Based on our research, rTMS deserves further considerations for its neuroprotective effect on EAE, and is an excellent candidate for further research and points that it could be used for more than for simple symptomatic therapy.",
publisher = "Elsevier BV",
journal = "Brain Research Bulletin",
title = "Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.",
volume = "162",
doi = "10.1016/j.brainresbull.2020.06.013",
pages = "208-217"
}

Dragić, M., Zeljković, M., Stevanović, I., Ilić, T., Ilić, N., Nedeljković, N.,& Ninković, M.. (2020). Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.. in Brain Research Bulletin
Elsevier BV., 162, 208-217.
https://doi.org/10.1016/j.brainresbull.2020.06.013

Dragić M, Zeljković M, Stevanović I, Ilić T, Ilić N, Nedeljković N, Ninković M. Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.. in Brain Research Bulletin. 2020;162:208-217.
doi:10.1016/j.brainresbull.2020.06.013 .

Dragić, Milorad, Zeljković, Milica, Stevanović, Ivana, Ilić, Tihomir, Ilić, Nela, Nedeljković, Nadezda, Ninković, Milica, "Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis." in Brain Research Bulletin, 162 (2020):208-217,
https://doi.org/10.1016/j.brainresbull.2020.06.013 . .

TY  - JOUR
AU  - Brisevac, Dusica
AU  - Bajić, Aleksandar
AU  - Bjelobaba, Ivana
AU  - Milosević, Milena B
AU  - Stojiljković, Mirjana B
AU  - Beyer, Cordian
AU  - Clarner, Tim
AU  - Kipp, Markus
AU  - Nedeljković, Nadezda N
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/958
AB  - Nucleoside triphosphate diphosphohydrolases (NTPDases) are ecto-enzymes catalyzing the first step of sequential hydrolysis of extracellular ATP to adenosine, as the final product. Among eight members of NTPDase family, NTPDases1-3 have been shown to be expressed in the brain. Although altered NTPDase expression has been observed in relation to cell death and reactive gliosis in several experimentally induced neuropathologies, regulators of NTPDases expression and function are largely unknown. The present study explored the effects of several inflammatory factors (i.e., INF-gamma, TNF-alpha, LPS, peroxide, and glutamate) on NTPDase1-3 activity and expression by cultured cortical astrocytes. We were able to demonstrate that INF-gamma and TNF-alpha increased both ATP and ADP hydrolysis, while LPS specifically increased ATP hydrolysis. Consistent with the observed enhanced nucleotidase activity, INF-gamma induced the upregulation of NTPDase1 at the mRNA and protein level. Furthermore, we were able to demonstrate that INF-gamma and TNF-alpha decreased the relative abundance of dominant astrocytic NTPDase2 in favor of NTPDase1. In summary, these results suggest that INF-gamma, TNF-alpha, and LPS may be relevant in vivo regulators of NTPDase expression in neuropathologies associated with neuroinflammation.
T2  - Journal of Molecular Neuroscience
T1  - Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro
IS  - 3
VL  - 51
SP  - 23
EP  - 879
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_958
ER  - 

@article{
author = "Brisevac, Dusica and Bajić, Aleksandar and Bjelobaba, Ivana and Milosević, Milena B and Stojiljković, Mirjana B and Beyer, Cordian and Clarner, Tim and Kipp, Markus and Nedeljković, Nadezda N",
year = "2013",
abstract = "Nucleoside triphosphate diphosphohydrolases (NTPDases) are ecto-enzymes catalyzing the first step of sequential hydrolysis of extracellular ATP to adenosine, as the final product. Among eight members of NTPDase family, NTPDases1-3 have been shown to be expressed in the brain. Although altered NTPDase expression has been observed in relation to cell death and reactive gliosis in several experimentally induced neuropathologies, regulators of NTPDases expression and function are largely unknown. The present study explored the effects of several inflammatory factors (i.e., INF-gamma, TNF-alpha, LPS, peroxide, and glutamate) on NTPDase1-3 activity and expression by cultured cortical astrocytes. We were able to demonstrate that INF-gamma and TNF-alpha increased both ATP and ADP hydrolysis, while LPS specifically increased ATP hydrolysis. Consistent with the observed enhanced nucleotidase activity, INF-gamma induced the upregulation of NTPDase1 at the mRNA and protein level. Furthermore, we were able to demonstrate that INF-gamma and TNF-alpha decreased the relative abundance of dominant astrocytic NTPDase2 in favor of NTPDase1. In summary, these results suggest that INF-gamma, TNF-alpha, and LPS may be relevant in vivo regulators of NTPDase expression in neuropathologies associated with neuroinflammation.",
journal = "Journal of Molecular Neuroscience",
title = "Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro",
number = "3",
volume = "51",
pages = "23-879",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_958"
}

Brisevac, D., Bajić, A., Bjelobaba, I., Milosević, M. B., Stojiljković, M. B., Beyer, C., Clarner, T., Kipp, M.,& Nedeljković, N. N.. (2013). Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro. in Journal of Molecular Neuroscience, 51(3), 23-879.
https://hdl.handle.net/21.15107/rcub_ibiss_958

Brisevac D, Bajić A, Bjelobaba I, Milosević MB, Stojiljković MB, Beyer C, Clarner T, Kipp M, Nedeljković NN. Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro. in Journal of Molecular Neuroscience. 2013;51(3):23-879.
https://hdl.handle.net/21.15107/rcub_ibiss_958 .

Brisevac, Dusica, Bajić, Aleksandar, Bjelobaba, Ivana, Milosević, Milena B, Stojiljković, Mirjana B, Beyer, Cordian, Clarner, Tim, Kipp, Markus, Nedeljković, Nadezda N, "Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro" in Journal of Molecular Neuroscience, 51, no. 3 (2013):23-879,
https://hdl.handle.net/21.15107/rcub_ibiss_958 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Bjelobaba, Ivana
AU  - Dačić, Sanja
AU  - Božić, Iva D.
AU  - Parabučki, Ana B.
AU  - Nedeljković, Nadezda N
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1167
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Microglia and astrocytes represent two related cell types involved in the brain pathology in EAE. Accumulations of hypertrophic reactive astrocytes, intensely stained with glial fibrillary acidic protein (GFAP), which also expressed vimentin, are prominent features of EAE lesions. Recent studies from our laboratory reported that ribavirin attenuated the disease process in EAE by reducing clinical and histological manifestations. EAE was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant. Real time PCR and immunohistochemistry were used for determination of GFAP and vimentin gene and tissue expression. We have observed the increased gene and tissue expression of GFAP and vimentin in EAE rats. Ribavirin treatment significantly decreased the number of reactive astrocytes at the peak of disease. At the end of the disease, we have observed reactive GFAP(+) and vimentin(+) astrocytes in both immunized and ribavirin-treated groups, accompanied by increased level of GFAP mRNA. The present study indicates that ribavirin may have the ability to attenuate astrocyte proliferation and glial scaring at the peak of the disease and modulate the astroglial response to EAE during the time-course of the disease.
T2  - Journal of Pharmacological Sciences
T1  - The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis
IS  - 3
VL  - 119
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1167
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Bjelobaba, Ivana and Dačić, Sanja and Božić, Iva D. and Parabučki, Ana B. and Nedeljković, Nadezda N and Peković, Sanja and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2012",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Microglia and astrocytes represent two related cell types involved in the brain pathology in EAE. Accumulations of hypertrophic reactive astrocytes, intensely stained with glial fibrillary acidic protein (GFAP), which also expressed vimentin, are prominent features of EAE lesions. Recent studies from our laboratory reported that ribavirin attenuated the disease process in EAE by reducing clinical and histological manifestations. EAE was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant. Real time PCR and immunohistochemistry were used for determination of GFAP and vimentin gene and tissue expression. We have observed the increased gene and tissue expression of GFAP and vimentin in EAE rats. Ribavirin treatment significantly decreased the number of reactive astrocytes at the peak of disease. At the end of the disease, we have observed reactive GFAP(+) and vimentin(+) astrocytes in both immunized and ribavirin-treated groups, accompanied by increased level of GFAP mRNA. The present study indicates that ribavirin may have the ability to attenuate astrocyte proliferation and glial scaring at the peak of the disease and modulate the astroglial response to EAE during the time-course of the disease.",
journal = "Journal of Pharmacological Sciences",
title = "The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis",
number = "3",
volume = "119",
pages = "232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1167"
}

Lavrnja, I., Savić, D., Bjelobaba, I., Dačić, S., Božić, I. D., Parabučki, A. B., Nedeljković, N. N., Peković, S., Rakić, L.,& Stojiljković, M. B.. (2012). The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis. in Journal of Pharmacological Sciences, 119(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1167

Lavrnja I, Savić D, Bjelobaba I, Dačić S, Božić ID, Parabučki AB, Nedeljković NN, Peković S, Rakić L, Stojiljković MB. The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis. in Journal of Pharmacological Sciences. 2012;119(3):null-232.
https://hdl.handle.net/21.15107/rcub_ibiss_1167 .

Lavrnja, Irena, Savić, Danijela, Bjelobaba, Ivana, Dačić, Sanja, Božić, Iva D., Parabučki, Ana B., Nedeljković, Nadezda N, Peković, Sanja, Rakić, Ljubisav, Stojiljković, Mirjana B, "The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis" in Journal of Pharmacological Sciences, 119, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1167 .

TY  - JOUR
AU  - Brisevac, Dusica
AU  - Bjelobaba, Ivana
AU  - Bajić, Aleksandar
AU  - Clarner, Tim
AU  - Stojiljković, Mirjana B
AU  - Beyer, Cordian
AU  - Anđus, Pavle R
AU  - Kipp, Markus
AU  - Nedeljković, Nadezda N
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1098
AB  - Ecto-5'-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), glutamate (Glu) and hydrogen peroxide (H2O2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-gamma, LPS, Glu and H2O2 decrease, while TNF-alpha increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Neurochemistry International
T1  - Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors
IS  - 5
VL  - 61
SP  - 406
EP  - 688
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1098
ER  - 

@article{
author = "Brisevac, Dusica and Bjelobaba, Ivana and Bajić, Aleksandar and Clarner, Tim and Stojiljković, Mirjana B and Beyer, Cordian and Anđus, Pavle R and Kipp, Markus and Nedeljković, Nadezda N",
year = "2012",
abstract = "Ecto-5'-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), glutamate (Glu) and hydrogen peroxide (H2O2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-gamma, LPS, Glu and H2O2 decrease, while TNF-alpha increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Neurochemistry International",
title = "Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors",
number = "5",
volume = "61",
pages = "406-688",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1098"
}

Brisevac, D., Bjelobaba, I., Bajić, A., Clarner, T., Stojiljković, M. B., Beyer, C., Anđus, P. R., Kipp, M.,& Nedeljković, N. N.. (2012). Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors. in Neurochemistry International, 61(5), 406-688.
https://hdl.handle.net/21.15107/rcub_ibiss_1098

Brisevac D, Bjelobaba I, Bajić A, Clarner T, Stojiljković MB, Beyer C, Anđus PR, Kipp M, Nedeljković NN. Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors. in Neurochemistry International. 2012;61(5):406-688.
https://hdl.handle.net/21.15107/rcub_ibiss_1098 .

Brisevac, Dusica, Bjelobaba, Ivana, Bajić, Aleksandar, Clarner, Tim, Stojiljković, Mirjana B, Beyer, Cordian, Anđus, Pavle R, Kipp, Markus, Nedeljković, Nadezda N, "Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors" in Neurochemistry International, 61, no. 5 (2012):406-688,
https://hdl.handle.net/21.15107/rcub_ibiss_1098 .

TY  - JOUR
AU  - Stanojević, Ivana
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadežda N.
AU  - Drakulić, Dunja R.
AU  - Petrović, Snježana B.
AU  - Stojiljković, Mirjana B.
AU  - Horvat, Anica I.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1286
AB  - Ecto-5'-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.
PB  - John Wiley and Sons
T2  - International Journal of Developmental Neuroscience
T1  - Ontogenetic profile of ecto-5 '-nucleotidase in rat brain synaptic plasma membranes
IS  - 4
VL  - 29
DO  - 10.1016/j.ijdevneu.2011.03.003
SP  - 397
EP  - 403
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1286
ER  - 

@article{
author = "Stanojević, Ivana and Bjelobaba, Ivana and Nedeljković, Nadežda N. and Drakulić, Dunja R. and Petrović, Snježana B. and Stojiljković, Mirjana B. and Horvat, Anica I.",
year = "2011",
abstract = "Ecto-5'-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.",
publisher = "John Wiley and Sons",
journal = "International Journal of Developmental Neuroscience",
title = "Ontogenetic profile of ecto-5 '-nucleotidase in rat brain synaptic plasma membranes",
number = "4",
volume = "29",
doi = "10.1016/j.ijdevneu.2011.03.003",
pages = "397-403",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1286"
}

Stanojević, I., Bjelobaba, I., Nedeljković, N. N., Drakulić, D. R., Petrović, S. B., Stojiljković, M. B.,& Horvat, A. I.. (2011). Ontogenetic profile of ecto-5 '-nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience
John Wiley and Sons., 29(4), 397-403.
https://doi.org/10.1016/j.ijdevneu.2011.03.003
https://hdl.handle.net/21.15107/rcub_ibiss_1286

Stanojević I, Bjelobaba I, Nedeljković NN, Drakulić DR, Petrović SB, Stojiljković MB, Horvat AI. Ontogenetic profile of ecto-5 '-nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience. 2011;29(4):397-403.
doi:10.1016/j.ijdevneu.2011.03.003
https://hdl.handle.net/21.15107/rcub_ibiss_1286 .

Stanojević, Ivana, Bjelobaba, Ivana, Nedeljković, Nadežda N., Drakulić, Dunja R., Petrović, Snježana B., Stojiljković, Mirjana B., Horvat, Anica I., "Ontogenetic profile of ecto-5 '-nucleotidase in rat brain synaptic plasma membranes" in International Journal of Developmental Neuroscience, 29, no. 4 (2011):397-403,
https://doi.org/10.1016/j.ijdevneu.2011.03.003 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1286 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Parabučki, Ana B.
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Dačić, Sanja
AU  - Peković, Sanja
AU  - Rakić, Ljubisa M
AU  - Stojiljković, Mirjana B
AU  - Nedeljković, Nadezda N
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1289
AB  - Traumatic injury induces massive release of ATP in the extracellular space, where it influences numerous aspects of neuronal, astrocytic, and microglial responses to injury by activating P2X and P2Y receptors. The extracellular ATP actions are controlled by the ectonucleotidase enzyme pathway, which hydrolyses ATP to adenosine at all neuronal and nonneuronal cell types. Adenosine activates its P1 receptors, which have important neuroprotective roles. The rate-limiting enzyme in the ectonucleotidase pathway is ecto-5 '-nucleotidase (e-5NT), which catalyzes the final step of dephosphorylation of AMP to adenosine. The aim of the present study was to characterize the expression pattern and cellular distribution of e-5NT in the perilesioned cortex at 4 hr and 1, 2, 7, and 15 days after unilateral cortical stab injury (CSI). Immunoblot and immunohistochemical studies showed that overall e-5NT expression was lower 4 hr and 1 day postinjury and then gradually increased above the control levels. Double-immunofluorescence studies further showed in control tissue the presence of the enzyme in the membranes surrounding neuronal somata and apical dendrites and less frequently in astrocytes. CSI caused a rapid (after 4 hr) and irreversible loss of the enzyme from neurons, accounting for a decrease in the overall enzyme expression. This was accompanied with a gradual increase in e-5NT-positive astrocytes, accounting for up-regulation of the enzyme levels in the injured area. Thus, CSI induced dynamic changes in the expression pattern of e-5NT that modify the ATP/adenosine ratio and the extent of P1 and P2 receptors activation and, therefore, outcome of the pathological processes after CSI. (C) 2011 Wiley-Liss, Inc.
T2  - Journal of Neuroscience Research
T1  - Dynamic Changes in the Expression Pattern of Ecto-5 '-Nucleotidase in the Rat Model of Cortical Stab Injury
IS  - 6
VL  - 89
EP  - 873
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1289
ER  - 

@article{
author = "Bjelobaba, Ivana and Parabučki, Ana B. and Lavrnja, Irena and Savić, Danijela and Dačić, Sanja and Peković, Sanja and Rakić, Ljubisa M and Stojiljković, Mirjana B and Nedeljković, Nadezda N",
year = "2011",
abstract = "Traumatic injury induces massive release of ATP in the extracellular space, where it influences numerous aspects of neuronal, astrocytic, and microglial responses to injury by activating P2X and P2Y receptors. The extracellular ATP actions are controlled by the ectonucleotidase enzyme pathway, which hydrolyses ATP to adenosine at all neuronal and nonneuronal cell types. Adenosine activates its P1 receptors, which have important neuroprotective roles. The rate-limiting enzyme in the ectonucleotidase pathway is ecto-5 '-nucleotidase (e-5NT), which catalyzes the final step of dephosphorylation of AMP to adenosine. The aim of the present study was to characterize the expression pattern and cellular distribution of e-5NT in the perilesioned cortex at 4 hr and 1, 2, 7, and 15 days after unilateral cortical stab injury (CSI). Immunoblot and immunohistochemical studies showed that overall e-5NT expression was lower 4 hr and 1 day postinjury and then gradually increased above the control levels. Double-immunofluorescence studies further showed in control tissue the presence of the enzyme in the membranes surrounding neuronal somata and apical dendrites and less frequently in astrocytes. CSI caused a rapid (after 4 hr) and irreversible loss of the enzyme from neurons, accounting for a decrease in the overall enzyme expression. This was accompanied with a gradual increase in e-5NT-positive astrocytes, accounting for up-regulation of the enzyme levels in the injured area. Thus, CSI induced dynamic changes in the expression pattern of e-5NT that modify the ATP/adenosine ratio and the extent of P1 and P2 receptors activation and, therefore, outcome of the pathological processes after CSI. (C) 2011 Wiley-Liss, Inc.",
journal = "Journal of Neuroscience Research",
title = "Dynamic Changes in the Expression Pattern of Ecto-5 '-Nucleotidase in the Rat Model of Cortical Stab Injury",
number = "6",
volume = "89",
pages = "873",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1289"
}

Bjelobaba, I., Parabučki, A. B., Lavrnja, I., Savić, D., Dačić, S., Peković, S., Rakić, L. M., Stojiljković, M. B.,& Nedeljković, N. N.. (2011). Dynamic Changes in the Expression Pattern of Ecto-5 '-Nucleotidase in the Rat Model of Cortical Stab Injury. in Journal of Neuroscience Research, 89(6).
https://hdl.handle.net/21.15107/rcub_ibiss_1289

Bjelobaba I, Parabučki AB, Lavrnja I, Savić D, Dačić S, Peković S, Rakić LM, Stojiljković MB, Nedeljković NN. Dynamic Changes in the Expression Pattern of Ecto-5 '-Nucleotidase in the Rat Model of Cortical Stab Injury. in Journal of Neuroscience Research. 2011;89(6):null-873.
https://hdl.handle.net/21.15107/rcub_ibiss_1289 .

Bjelobaba, Ivana, Parabučki, Ana B., Lavrnja, Irena, Savić, Danijela, Dačić, Sanja, Peković, Sanja, Rakić, Ljubisa M, Stojiljković, Mirjana B, Nedeljković, Nadezda N, "Dynamic Changes in the Expression Pattern of Ecto-5 '-Nucleotidase in the Rat Model of Cortical Stab Injury" in Journal of Neuroscience Research, 89, no. 6 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1289 .

TY  - CONF
AU  - Parabučki, Ana B.
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
AU  - Nedeljković, Nadezda N
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1263
C3  - Glia
T1  - Pannexin-1 Upregulation in Cultured Astrocytes Is Induced By Scratch Wound
IS  - null
VL  - 59
EP  - S147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1263
ER  - 

@conference{
author = "Parabučki, Ana B. and Bjelobaba, Ivana and Savić, Danijela and Stojiljković, Mirjana B and Peković, Sanja and Nedeljković, Nadezda N",
year = "2011",
journal = "Glia",
title = "Pannexin-1 Upregulation in Cultured Astrocytes Is Induced By Scratch Wound",
number = "null",
volume = "59",
pages = "S147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1263"
}

Parabučki, A. B., Bjelobaba, I., Savić, D., Stojiljković, M. B., Peković, S.,& Nedeljković, N. N.. (2011). Pannexin-1 Upregulation in Cultured Astrocytes Is Induced By Scratch Wound. in Glia, 59(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1263

Parabučki AB, Bjelobaba I, Savić D, Stojiljković MB, Peković S, Nedeljković NN. Pannexin-1 Upregulation in Cultured Astrocytes Is Induced By Scratch Wound. in Glia. 2011;59(null):null-S147.
https://hdl.handle.net/21.15107/rcub_ibiss_1263 .

Parabučki, Ana B., Bjelobaba, Ivana, Savić, Danijela, Stojiljković, Mirjana B, Peković, Sanja, Nedeljković, Nadezda N, "Pannexin-1 Upregulation in Cultured Astrocytes Is Induced By Scratch Wound" in Glia, 59, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1263 .

TY  - CONF
AU  - Dačić, Sanja
AU  - Stojiljković, Mirjana B
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Parabučki, Ana B.
AU  - Božić, Iva D.
AU  - Bjelobaba, Ivana
AU  - Laketa, Danijela
AU  - Nedeljković, Nadezda N
AU  - Rakić, Ljubisa M
AU  - Peković, Sanja
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1262
C3  - Glia
T1  - Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation
IS  - null
VL  - 59
EP  - S52
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1262
ER  - 

@conference{
author = "Dačić, Sanja and Stojiljković, Mirjana B and Lavrnja, Irena and Savić, Danijela and Parabučki, Ana B. and Božić, Iva D. and Bjelobaba, Ivana and Laketa, Danijela and Nedeljković, Nadezda N and Rakić, Ljubisa M and Peković, Sanja",
year = "2011",
journal = "Glia",
title = "Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation",
number = "null",
volume = "59",
pages = "S52",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1262"
}

Dačić, S., Stojiljković, M. B., Lavrnja, I., Savić, D., Parabučki, A. B., Božić, I. D., Bjelobaba, I., Laketa, D., Nedeljković, N. N., Rakić, L. M.,& Peković, S.. (2011). Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation. in Glia, 59(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1262

Dačić S, Stojiljković MB, Lavrnja I, Savić D, Parabučki AB, Božić ID, Bjelobaba I, Laketa D, Nedeljković NN, Rakić LM, Peković S. Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation. in Glia. 2011;59(null):null-S52.
https://hdl.handle.net/21.15107/rcub_ibiss_1262 .

Dačić, Sanja, Stojiljković, Mirjana B, Lavrnja, Irena, Savić, Danijela, Parabučki, Ana B., Božić, Iva D., Bjelobaba, Ivana, Laketa, Danijela, Nedeljković, Nadezda N, Rakić, Ljubisa M, Peković, Sanja, "Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation" in Glia, 59, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1262 .

TY  - CONF
AU  - Savić, Danijela
AU  - Stojiljković, Mirjana B
AU  - Lavrnja, Irena
AU  - Parabučki, Ana B.
AU  - Dačić, Sanja
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadezda N
AU  - Herdegen, T
AU  - Peković, Sanja
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1264
C3  - Glia
T1  - In Vitro Effect of Ribavirin and Tiazofurin on Microglial Response To Inflammation
IS  - null
VL  - 59
EP  - S149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1264
ER  - 

@conference{
author = "Savić, Danijela and Stojiljković, Mirjana B and Lavrnja, Irena and Parabučki, Ana B. and Dačić, Sanja and Bjelobaba, Ivana and Nedeljković, Nadezda N and Herdegen, T and Peković, Sanja",
year = "2011",
journal = "Glia",
title = "In Vitro Effect of Ribavirin and Tiazofurin on Microglial Response To Inflammation",
number = "null",
volume = "59",
pages = "S149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1264"
}

Savić, D., Stojiljković, M. B., Lavrnja, I., Parabučki, A. B., Dačić, S., Bjelobaba, I., Nedeljković, N. N., Herdegen, T.,& Peković, S.. (2011). In Vitro Effect of Ribavirin and Tiazofurin on Microglial Response To Inflammation. in Glia, 59(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1264

Savić D, Stojiljković MB, Lavrnja I, Parabučki AB, Dačić S, Bjelobaba I, Nedeljković NN, Herdegen T, Peković S. In Vitro Effect of Ribavirin and Tiazofurin on Microglial Response To Inflammation. in Glia. 2011;59(null):null-S149.
https://hdl.handle.net/21.15107/rcub_ibiss_1264 .

Savić, Danijela, Stojiljković, Mirjana B, Lavrnja, Irena, Parabučki, Ana B., Dačić, Sanja, Bjelobaba, Ivana, Nedeljković, Nadezda N, Herdegen, T, Peković, Sanja, "In Vitro Effect of Ribavirin and Tiazofurin on Microglial Response To Inflammation" in Glia, 59, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1264 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Parabučki, Ana B.
AU  - Savić, Danijela
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
AU  - Nedeljković, Nadezda N
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1341
AB  - The ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3), an enzyme involved in degradation of extracellular adenosine triphosphate (ATP), is expressed on nerve fibers in different brain regions, including cortex. Here we studied the expression and role of this enzyme after unilateral cortical stab injury in rats. In cortical sections of control rats, NTPDase3 immunoreactivity was associated with two types of fibers: thin processes, occasionally with small mushroom-like protrusions and slightly thicker fibers with more pronounced and more frequent varicosities, whereas immunopositive neuronal perycaria were never observed. Although NTPDase3-positive thin processes and thicker fibers, by general appearance, size and shape, could be dendrites and axons, respectively, they were never immunopositive for microtubule associated protein-2 or neurofilament H subunit. Cortical stab injury induced rapid (within 4 hours) focal varicose swelling that evolved over time to prominent beading of NTPDase3-positive fibers. The NTPDase3-positive fibers in all experimental groups also abundantly express NTPDase1, ecto-5'-nucleotidase and P2X2 receptor channels. Because the brain injury causes a massive ATP release, it is reasonable to conclude that purinoreceptors and ectonucleotidases play an important role in the process of neuritic beading. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - The Cortical Stab Injury Induces Beading of Fibers Expressing Ecto-Nucleoside Triphosphate Diphosphohydrolase 3
IS  - 1
VL  - 170
EP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1341
ER  - 

@article{
author = "Bjelobaba, Ivana and Lavrnja, Irena and Parabučki, Ana B. and Savić, Danijela and Stojiljković, Mirjana B and Peković, Sanja and Nedeljković, Nadezda N",
year = "2010",
abstract = "The ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3), an enzyme involved in degradation of extracellular adenosine triphosphate (ATP), is expressed on nerve fibers in different brain regions, including cortex. Here we studied the expression and role of this enzyme after unilateral cortical stab injury in rats. In cortical sections of control rats, NTPDase3 immunoreactivity was associated with two types of fibers: thin processes, occasionally with small mushroom-like protrusions and slightly thicker fibers with more pronounced and more frequent varicosities, whereas immunopositive neuronal perycaria were never observed. Although NTPDase3-positive thin processes and thicker fibers, by general appearance, size and shape, could be dendrites and axons, respectively, they were never immunopositive for microtubule associated protein-2 or neurofilament H subunit. Cortical stab injury induced rapid (within 4 hours) focal varicose swelling that evolved over time to prominent beading of NTPDase3-positive fibers. The NTPDase3-positive fibers in all experimental groups also abundantly express NTPDase1, ecto-5'-nucleotidase and P2X2 receptor channels. Because the brain injury causes a massive ATP release, it is reasonable to conclude that purinoreceptors and ectonucleotidases play an important role in the process of neuritic beading. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "The Cortical Stab Injury Induces Beading of Fibers Expressing Ecto-Nucleoside Triphosphate Diphosphohydrolase 3",
number = "1",
volume = "170",
pages = "116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1341"
}

Bjelobaba, I., Lavrnja, I., Parabučki, A. B., Savić, D., Stojiljković, M. B., Peković, S.,& Nedeljković, N. N.. (2010). The Cortical Stab Injury Induces Beading of Fibers Expressing Ecto-Nucleoside Triphosphate Diphosphohydrolase 3. in Neuroscience, 170(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1341

Bjelobaba I, Lavrnja I, Parabučki AB, Savić D, Stojiljković MB, Peković S, Nedeljković NN. The Cortical Stab Injury Induces Beading of Fibers Expressing Ecto-Nucleoside Triphosphate Diphosphohydrolase 3. in Neuroscience. 2010;170(1):null-116.
https://hdl.handle.net/21.15107/rcub_ibiss_1341 .

Bjelobaba, Ivana, Lavrnja, Irena, Parabučki, Ana B., Savić, Danijela, Stojiljković, Mirjana B, Peković, Sanja, Nedeljković, Nadezda N, "The Cortical Stab Injury Induces Beading of Fibers Expressing Ecto-Nucleoside Triphosphate Diphosphohydrolase 3" in Neuroscience, 170, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1341 .

TY  - CONF
AU  - Nedeljković, Nadezda N
AU  - Bjelobaba, Ivana
AU  - Parabučki, Ana B.
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Stojiljković, Mirjana B
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1374
C3  - Purinergic Signalling
T1  - Ecto-5 '-nucleotidase (e-5NT) switches from neurons to astrocytes after cortical stab injury in rat
IS  - null
VL  - 6
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1374
ER  - 

@conference{
author = "Nedeljković, Nadezda N and Bjelobaba, Ivana and Parabučki, Ana B. and Lavrnja, Irena and Peković, Sanja and Stojiljković, Mirjana B",
year = "2010",
journal = "Purinergic Signalling",
title = "Ecto-5 '-nucleotidase (e-5NT) switches from neurons to astrocytes after cortical stab injury in rat",
number = "null",
volume = "6",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1374"
}

Nedeljković, N. N., Bjelobaba, I., Parabučki, A. B., Lavrnja, I., Peković, S.,& Stojiljković, M. B.. (2010). Ecto-5 '-nucleotidase (e-5NT) switches from neurons to astrocytes after cortical stab injury in rat. in Purinergic Signalling, 6(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1374

Nedeljković NN, Bjelobaba I, Parabučki AB, Lavrnja I, Peković S, Stojiljković MB. Ecto-5 '-nucleotidase (e-5NT) switches from neurons to astrocytes after cortical stab injury in rat. in Purinergic Signalling. 2010;6(null):null-77.
https://hdl.handle.net/21.15107/rcub_ibiss_1374 .

Nedeljković, Nadezda N, Bjelobaba, Ivana, Parabučki, Ana B., Lavrnja, Irena, Peković, Sanja, Stojiljković, Mirjana B, "Ecto-5 '-nucleotidase (e-5NT) switches from neurons to astrocytes after cortical stab injury in rat" in Purinergic Signalling, 6, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1374 .

TY  - JOUR
AU  - Laketa, Danijela
AU  - Bjelobaba, Ivana
AU  - Savić, Jasmina Z
AU  - Lavrnja, Irena
AU  - Stojiljković, Mirjana B
AU  - Rakić, Ljubisav
AU  - Nedeljković, Nadezda N
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1372
AB  - Biochemical properties of nucleotide pyrophosphatase/phosphodiesterase (NPP) in rat serum have been described by assessing its nucleotide phosphodiesterase activity, using p-nitrophenyl-5'-thymidine monophosphate (p-Nph-5'-TMP) as a substrate. It was demonstrated that NPP activity shares some typical characteristics described for other soluble NPP, such as divalent cation dependence, strong alkaline pH optimum (pH 10.5), inhibition by glycosaminoglycans, and K (m) for p-Nph-5'-TMP hydrolysis of 61.8 +/- A 5.2 mu M. In order to characterize the relation between phosphodiesterase and pyrophosphatase activities of NPP, we have analyzed the effects of different natural nucleotides and nucleotide analogs. ATP, ADP, and AMP competitively inhibited p-Nph-5'-TMP hydrolysis with K (i) values ranging 13-43 mu M. Nucleotide analogs, alpha,beta-metATP, BzATP, 2-MeSATP, and dialATP behaved as competitive inhibitors, whereas alpha,beta-metADP induced mixed inhibition, with K (i) ranging from 2 to 20 mu M. Chromatographic analysis revealed that alpha,beta-metATP, BzATP, and 2-MeSATP were catalytically degraded in the serum, whereas dialATP and alpha,beta-metADP resisted hydrolysis, implying that the former act as substrates and the latter as true competitive inhibitors of serum NPP activity. Since NPP activity is involved in generation, breakdown, and recycling of extracellular adenine nucleotides in the vascular compartment, the results suggest that both hydrolyzable and non-hydrolyzable nucleotide analogs could alter the amplitude and direction of ATP actions and could have potential therapeutic application.
T2  - Molecular and Cellular Biochemistry
T1  - Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum
IS  - 1-2
VL  - 339
EP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1372
ER  - 

@article{
author = "Laketa, Danijela and Bjelobaba, Ivana and Savić, Jasmina Z and Lavrnja, Irena and Stojiljković, Mirjana B and Rakić, Ljubisav and Nedeljković, Nadezda N",
year = "2010",
abstract = "Biochemical properties of nucleotide pyrophosphatase/phosphodiesterase (NPP) in rat serum have been described by assessing its nucleotide phosphodiesterase activity, using p-nitrophenyl-5'-thymidine monophosphate (p-Nph-5'-TMP) as a substrate. It was demonstrated that NPP activity shares some typical characteristics described for other soluble NPP, such as divalent cation dependence, strong alkaline pH optimum (pH 10.5), inhibition by glycosaminoglycans, and K (m) for p-Nph-5'-TMP hydrolysis of 61.8 +/- A 5.2 mu M. In order to characterize the relation between phosphodiesterase and pyrophosphatase activities of NPP, we have analyzed the effects of different natural nucleotides and nucleotide analogs. ATP, ADP, and AMP competitively inhibited p-Nph-5'-TMP hydrolysis with K (i) values ranging 13-43 mu M. Nucleotide analogs, alpha,beta-metATP, BzATP, 2-MeSATP, and dialATP behaved as competitive inhibitors, whereas alpha,beta-metADP induced mixed inhibition, with K (i) ranging from 2 to 20 mu M. Chromatographic analysis revealed that alpha,beta-metATP, BzATP, and 2-MeSATP were catalytically degraded in the serum, whereas dialATP and alpha,beta-metADP resisted hydrolysis, implying that the former act as substrates and the latter as true competitive inhibitors of serum NPP activity. Since NPP activity is involved in generation, breakdown, and recycling of extracellular adenine nucleotides in the vascular compartment, the results suggest that both hydrolyzable and non-hydrolyzable nucleotide analogs could alter the amplitude and direction of ATP actions and could have potential therapeutic application.",
journal = "Molecular and Cellular Biochemistry",
title = "Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum",
number = "1-2",
volume = "339",
pages = "106",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1372"
}

Laketa, D., Bjelobaba, I., Savić, J. Z., Lavrnja, I., Stojiljković, M. B., Rakić, L.,& Nedeljković, N. N.. (2010). Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum. in Molecular and Cellular Biochemistry, 339(1-2).
https://hdl.handle.net/21.15107/rcub_ibiss_1372

Laketa D, Bjelobaba I, Savić JZ, Lavrnja I, Stojiljković MB, Rakić L, Nedeljković NN. Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum. in Molecular and Cellular Biochemistry. 2010;339(1-2):null-106.
https://hdl.handle.net/21.15107/rcub_ibiss_1372 .

Laketa, Danijela, Bjelobaba, Ivana, Savić, Jasmina Z, Lavrnja, Irena, Stojiljković, Mirjana B, Rakić, Ljubisav, Nedeljković, Nadezda N, "Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum" in Molecular and Cellular Biochemistry, 339, no. 1-2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1372 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Stojiljković, Mirjana B
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Peković, Sanja
AU  - Dačić, Sanja
AU  - Laketa, Danijela
AU  - Rakić, Ljubisav
AU  - Nedeljković, Nadezda N
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1473
AB  - During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering local cellular and biochemical responses to injury. Different models of brain injury reveal that the local increase in adenine nucleotides levels is followed by a compensatory up-regulation of ectonucleotidase enzymes that catalyze sequential hydrolysis of ATP to ADP, AMP and adenosine. However, recent studies imply that changes in adenine nucleotides release may also occur in the areas distant from the site of direct damage. Therefore, in the present study we have used the model of cortical stab injury to analyze extracellular ATP, ADP and AMP hydrolysis in the membrane preparations obtained from the brain regions that were not subjected to direct tissue damage. The brain regions analyzed were contralateral cortex, hippocampus, caudate nucleus, thalamus and hypothalamus. It was evidenced that cortical stab injury induced early widespread decrease in AMP hydrolysis in all brain areas tested, except in the hypothalamus, without changes in ATP hydrolysis. These findings imply that brain injury affects global extracellular adenine nucleotide and nucleoside levels, consequently affecting neuronal function in the regions distant to the primary damage.
T2  - General Physiology and Biophysics
T1  - Regional changes in ectonucleotidase activity after cortical stab injury in rat
IS  - null
VL  - 28
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1473
ER  - 

@article{
author = "Bjelobaba, Ivana and Stojiljković, Mirjana B and Lavrnja, Irena and Savić, Danijela and Peković, Sanja and Dačić, Sanja and Laketa, Danijela and Rakić, Ljubisav and Nedeljković, Nadezda N",
year = "2009",
abstract = "During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering local cellular and biochemical responses to injury. Different models of brain injury reveal that the local increase in adenine nucleotides levels is followed by a compensatory up-regulation of ectonucleotidase enzymes that catalyze sequential hydrolysis of ATP to ADP, AMP and adenosine. However, recent studies imply that changes in adenine nucleotides release may also occur in the areas distant from the site of direct damage. Therefore, in the present study we have used the model of cortical stab injury to analyze extracellular ATP, ADP and AMP hydrolysis in the membrane preparations obtained from the brain regions that were not subjected to direct tissue damage. The brain regions analyzed were contralateral cortex, hippocampus, caudate nucleus, thalamus and hypothalamus. It was evidenced that cortical stab injury induced early widespread decrease in AMP hydrolysis in all brain areas tested, except in the hypothalamus, without changes in ATP hydrolysis. These findings imply that brain injury affects global extracellular adenine nucleotide and nucleoside levels, consequently affecting neuronal function in the regions distant to the primary damage.",
journal = "General Physiology and Biophysics",
title = "Regional changes in ectonucleotidase activity after cortical stab injury in rat",
number = "null",
volume = "28",
pages = "68",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1473"
}

Bjelobaba, I., Stojiljković, M. B., Lavrnja, I., Savić, D., Peković, S., Dačić, S., Laketa, D., Rakić, L.,& Nedeljković, N. N.. (2009). Regional changes in ectonucleotidase activity after cortical stab injury in rat. in General Physiology and Biophysics, 28(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1473

Bjelobaba I, Stojiljković MB, Lavrnja I, Savić D, Peković S, Dačić S, Laketa D, Rakić L, Nedeljković NN. Regional changes in ectonucleotidase activity after cortical stab injury in rat. in General Physiology and Biophysics. 2009;28(null):null-68.
https://hdl.handle.net/21.15107/rcub_ibiss_1473 .

Bjelobaba, Ivana, Stojiljković, Mirjana B, Lavrnja, Irena, Savić, Danijela, Peković, Sanja, Dačić, Sanja, Laketa, Danijela, Rakić, Ljubisav, Nedeljković, Nadezda N, "Regional changes in ectonucleotidase activity after cortical stab injury in rat" in General Physiology and Biophysics, 28, no. null (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1473 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Nedeljković, Nadezda N
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Dačić, Sanja
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Stojiljković, Mirjana B
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1474
AB  - The role of extracellular purines and purinoreceptors in the pathophysiology of different neurological disorders is the focus of rapidly expanding area of research. Ectonucleotidases are the enzymes with multiple roles in extracellular nucleotides metabolism and regulation of nucleotide-based intercellular signaling. The aim of present study was to investigate the changes in the ATP, ADP and AMP hydrolyzing activities after ribavirin treatment in spinal cord during experimental autoimmune encephalomyelitis (EAE). Our results demonstrate that ribavirin itself had no significant effect on ectoenzyme activities, when tested in vitro and in vivo on spinal cord crude membrane preparation of intact animals. We observed significant increase in ATP, ADP and AMP hydrolyzing activity in the spinal cord crude membrane preparation in EAE animals at 15 days post immunization compared to control animals. The increase was registered at 28 days post immunization, as well. At same time points, ribavirin treatment decreased ATP, ADP and AMP hydrolyzing activity compared to EAE animals. In addition, no significant changes 8 days post immunization was observed between EAE-induced and ribavirin- treated EAE animals and these levels were similar to control level. Thus, we suppose that ribavirin-induced alteration in ectonucleotidase activities is rather due to its suppression of inflammation, than to its direct action on ATP, ADP and AMP hydrolysis.
T2  - General Physiology and Biophysics
T1  - Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis
IS  - null
VL  - 28
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1474
ER  - 

@article{
author = "Lavrnja, Irena and Nedeljković, Nadezda N and Bjelobaba, Ivana and Savić, Danijela and Dačić, Sanja and Peković, Sanja and Rakić, Ljubisav and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Stojiljković, Mirjana B",
year = "2009",
abstract = "The role of extracellular purines and purinoreceptors in the pathophysiology of different neurological disorders is the focus of rapidly expanding area of research. Ectonucleotidases are the enzymes with multiple roles in extracellular nucleotides metabolism and regulation of nucleotide-based intercellular signaling. The aim of present study was to investigate the changes in the ATP, ADP and AMP hydrolyzing activities after ribavirin treatment in spinal cord during experimental autoimmune encephalomyelitis (EAE). Our results demonstrate that ribavirin itself had no significant effect on ectoenzyme activities, when tested in vitro and in vivo on spinal cord crude membrane preparation of intact animals. We observed significant increase in ATP, ADP and AMP hydrolyzing activity in the spinal cord crude membrane preparation in EAE animals at 15 days post immunization compared to control animals. The increase was registered at 28 days post immunization, as well. At same time points, ribavirin treatment decreased ATP, ADP and AMP hydrolyzing activity compared to EAE animals. In addition, no significant changes 8 days post immunization was observed between EAE-induced and ribavirin- treated EAE animals and these levels were similar to control level. Thus, we suppose that ribavirin-induced alteration in ectonucleotidase activities is rather due to its suppression of inflammation, than to its direct action on ATP, ADP and AMP hydrolysis.",
journal = "General Physiology and Biophysics",
title = "Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis",
number = "null",
volume = "28",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1474"
}

Lavrnja, I., Nedeljković, N. N., Bjelobaba, I., Savić, D., Dačić, S., Peković, S., Rakić, L., Mostarica-Stojković, M. B., Stošić-Grujičić, S.,& Stojiljković, M. B.. (2009). Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis. in General Physiology and Biophysics, 28(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1474

Lavrnja I, Nedeljković NN, Bjelobaba I, Savić D, Dačić S, Peković S, Rakić L, Mostarica-Stojković MB, Stošić-Grujičić S, Stojiljković MB. Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis. in General Physiology and Biophysics. 2009;28(null):null-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1474 .

Lavrnja, Irena, Nedeljković, Nadezda N, Bjelobaba, Ivana, Savić, Danijela, Dačić, Sanja, Peković, Sanja, Rakić, Ljubisav, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Stojiljković, Mirjana B, "Ribavirin administration alters ectonudeotidase activities in experimental autoimmune encephalomyelitis" in General Physiology and Biophysics, 28, no. null (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1474 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Bjelobaba, Ivana
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Nedeljković, Nadezda N
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1436
AB  - The aim of the present study was to analyze the activities of extracellular purine metabolizing enzymes, CD39 (apyrase, EC 3.6.1.5) and CD73 (ecto-5' nucleotidase, EC 3.1.3.5) in experimental autoimmune encephalomyelitis (EAE). The levels of ATP, ADP and AMP hydrolysis were analyzed in the blood serum and in the rat spinal cord plasma membrane preparation 8, 15 and 25 days after induction of EAE. The animals were divided in three groups: control (saline), CFA (adjuvant-only) and EAE (CFA and homogenate of spinal cords). Eight days after immunization, ATP, ADP and AMP hydrolysis in the blood serum and spinal cord membrane preparations were unaffected in EAE compared to both, control and CFA group. In the peak of disease, ATP, ADP and AMP hydrolysis in EAE group showed significant decrease in the blood serum and prominent increase in the spinal cord membrane preparation compared to CFA and control group. At the end of illness, as judged by disappearance of clinical manifestation of EAE, ATP, ADP and AMP hydrolysis, although closer to CFA levels, were still significantly different in respect to the CFA group. Modulation of ATP, ADP and AMP hydrolysis suggests that they operate during EAE and might represent the basis of novel therapeutic strategies in immune-mediated diseases, such as MS. (C) 2009 Elsevier Ltd. All rights reserved.
T2  - Neurochemistry International
T1  - Time-course changes in ectonucleotidase activities during experimental autoimmune encephalomyelitis
IS  - 4
VL  - 55
EP  - 198
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1436
ER  - 

@article{
author = "Lavrnja, Irena and Bjelobaba, Ivana and Stojiljković, Mirjana B and Peković, Sanja and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Nedeljković, Nadezda N",
year = "2009",
abstract = "The aim of the present study was to analyze the activities of extracellular purine metabolizing enzymes, CD39 (apyrase, EC 3.6.1.5) and CD73 (ecto-5' nucleotidase, EC 3.1.3.5) in experimental autoimmune encephalomyelitis (EAE). The levels of ATP, ADP and AMP hydrolysis were analyzed in the blood serum and in the rat spinal cord plasma membrane preparation 8, 15 and 25 days after induction of EAE. The animals were divided in three groups: control (saline), CFA (adjuvant-only) and EAE (CFA and homogenate of spinal cords). Eight days after immunization, ATP, ADP and AMP hydrolysis in the blood serum and spinal cord membrane preparations were unaffected in EAE compared to both, control and CFA group. In the peak of disease, ATP, ADP and AMP hydrolysis in EAE group showed significant decrease in the blood serum and prominent increase in the spinal cord membrane preparation compared to CFA and control group. At the end of illness, as judged by disappearance of clinical manifestation of EAE, ATP, ADP and AMP hydrolysis, although closer to CFA levels, were still significantly different in respect to the CFA group. Modulation of ATP, ADP and AMP hydrolysis suggests that they operate during EAE and might represent the basis of novel therapeutic strategies in immune-mediated diseases, such as MS. (C) 2009 Elsevier Ltd. All rights reserved.",
journal = "Neurochemistry International",
title = "Time-course changes in ectonucleotidase activities during experimental autoimmune encephalomyelitis",
number = "4",
volume = "55",
pages = "198",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1436"
}

Lavrnja, I., Bjelobaba, I., Stojiljković, M. B., Peković, S., Mostarica-Stojković, M. B., Stošić-Grujičić, S.,& Nedeljković, N. N.. (2009). Time-course changes in ectonucleotidase activities during experimental autoimmune encephalomyelitis. in Neurochemistry International, 55(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1436

Lavrnja I, Bjelobaba I, Stojiljković MB, Peković S, Mostarica-Stojković MB, Stošić-Grujičić S, Nedeljković NN. Time-course changes in ectonucleotidase activities during experimental autoimmune encephalomyelitis. in Neurochemistry International. 2009;55(4):null-198.
https://hdl.handle.net/21.15107/rcub_ibiss_1436 .

Lavrnja, Irena, Bjelobaba, Ivana, Stojiljković, Mirjana B, Peković, Sanja, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Nedeljković, Nadezda N, "Time-course changes in ectonucleotidase activities during experimental autoimmune encephalomyelitis" in Neurochemistry International, 55, no. 4 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1436 .

TY  - JOUR
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Bjelobaba, Ivana
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Jovanović, Saša
AU  - Nedeljković, Nadežda N.
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1534
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) and the helpful tool in preclinical testing of various substances considered for treatment of this human CNS disease. Ribavirin (R) and tiazofurin (T) are purine nucleoside analogues, with the broad spectrum of anti-viral, anti-tumoral and anti-inflammatory properties. We proposed that combined treatment with RT, administrated during the effector phase of EAE, would attenuate disease severity, both clinically and pathologically. Ribavirin was given daily at a dosage of 30 mg/kg and tiazofurin was given at a dosage of 10 mg/kg every other day for 15 days. We detected amelioration of clinical signs and faster recovery in the RT group compared to the control group. Immunohistochemical analyses revealed that RT treatment decrease the number of T cells, macrophages and microglia. In the controls, we detected reactive type of microglia, while in the RT group we noticed ramified/resting form. Demyelination areas and axonal damage were not recorded in the RT group, in contrast to the control group where multiple areas of demyelination zones and axonal loss were found. RT combination treatment suppresses ongoing EAE, prevents demyelination and axonal loss, and therefore may well be the potential therapy for the treatment of MS. (C) 2007 Elsevier B.V. All rights reserved.
PB  - New York, USA: Springer - Plenum publishers
T2  - Journal of the Neurological Sciences
T1  - Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation
IS  - 1-2
VL  - 267
DO  - 10.1016/j.jns.2007.10.010
SP  - 76
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1534
ER  - 

@article{
author = "Savić, Danijela and Lavrnja, Irena and Peković, Sanja and Dacić, Sanja and Bjelobaba, Ivana and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Jovanović, Saša and Nedeljković, Nadežda N. and Rakić, Ljubisav and Stojiljković, Mirjana B.",
year = "2008",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) and the helpful tool in preclinical testing of various substances considered for treatment of this human CNS disease. Ribavirin (R) and tiazofurin (T) are purine nucleoside analogues, with the broad spectrum of anti-viral, anti-tumoral and anti-inflammatory properties. We proposed that combined treatment with RT, administrated during the effector phase of EAE, would attenuate disease severity, both clinically and pathologically. Ribavirin was given daily at a dosage of 30 mg/kg and tiazofurin was given at a dosage of 10 mg/kg every other day for 15 days. We detected amelioration of clinical signs and faster recovery in the RT group compared to the control group. Immunohistochemical analyses revealed that RT treatment decrease the number of T cells, macrophages and microglia. In the controls, we detected reactive type of microglia, while in the RT group we noticed ramified/resting form. Demyelination areas and axonal damage were not recorded in the RT group, in contrast to the control group where multiple areas of demyelination zones and axonal loss were found. RT combination treatment suppresses ongoing EAE, prevents demyelination and axonal loss, and therefore may well be the potential therapy for the treatment of MS. (C) 2007 Elsevier B.V. All rights reserved.",
publisher = "New York, USA: Springer - Plenum publishers",
journal = "Journal of the Neurological Sciences",
title = "Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation",
number = "1-2",
volume = "267",
doi = "10.1016/j.jns.2007.10.010",
pages = "76-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1534"
}

Savić, D., Lavrnja, I., Peković, S., Dacić, S., Bjelobaba, I., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Jovanović, S., Nedeljković, N. N., Rakić, L.,& Stojiljković, M. B.. (2008). Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation. in Journal of the Neurological Sciences
New York, USA: Springer - Plenum publishers., 267(1-2), 76-85.
https://doi.org/10.1016/j.jns.2007.10.010
https://hdl.handle.net/21.15107/rcub_ibiss_1534

Savić D, Lavrnja I, Peković S, Dacić S, Bjelobaba I, Mostarica-Stojković MB, Stošić-Grujičić S, Jovanović S, Nedeljković NN, Rakić L, Stojiljković MB. Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation. in Journal of the Neurological Sciences. 2008;267(1-2):76-85.
doi:10.1016/j.jns.2007.10.010
https://hdl.handle.net/21.15107/rcub_ibiss_1534 .

Savić, Danijela, Lavrnja, Irena, Peković, Sanja, Dacić, Sanja, Bjelobaba, Ivana, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Jovanović, Saša, Nedeljković, Nadežda N., Rakić, Ljubisav, Stojiljković, Mirjana B., "Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation" in Journal of the Neurological Sciences, 267, no. 1-2 (2008):76-85,
https://doi.org/10.1016/j.jns.2007.10.010 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1534 .

TY  - JOUR
AU  - Nedeljković, Nadežda N.
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1533
AB  - During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.
PB  - Amsterdam, Netherlands: Elsevier
T2  - Neurochemical Research
T1  - Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat
IS  - 5
VL  - 33
DO  - 10.1007/s11064-007-9529-0
SP  - 873
EP  - 879
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1533
ER  - 

@article{
author = "Nedeljković, Nadežda N. and Bjelobaba, Ivana and Lavrnja, Irena and Savić, Danijela and Peković, Sanja and Rakić, Ljubisav and Stojiljković, Mirjana B.",
year = "2008",
abstract = "During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "Neurochemical Research",
title = "Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat",
number = "5",
volume = "33",
doi = "10.1007/s11064-007-9529-0",
pages = "873-879",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1533"
}

Nedeljković, N. N., Bjelobaba, I., Lavrnja, I., Savić, D., Peković, S., Rakić, L.,& Stojiljković, M. B.. (2008). Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat. in Neurochemical Research
Amsterdam, Netherlands: Elsevier., 33(5), 873-879.
https://doi.org/10.1007/s11064-007-9529-0
https://hdl.handle.net/21.15107/rcub_ibiss_1533

Nedeljković NN, Bjelobaba I, Lavrnja I, Savić D, Peković S, Rakić L, Stojiljković MB. Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat. in Neurochemical Research. 2008;33(5):873-879.
doi:10.1007/s11064-007-9529-0
https://hdl.handle.net/21.15107/rcub_ibiss_1533 .

Nedeljković, Nadežda N., Bjelobaba, Ivana, Lavrnja, Irena, Savić, Danijela, Peković, Sanja, Rakić, Ljubisav, Stojiljković, Mirjana B., "Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat" in Neurochemical Research, 33, no. 5 (2008):873-879,
https://doi.org/10.1007/s11064-007-9529-0 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1533 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Bjelobaba, Ivana
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Nedeljković, Nadežda N.
AU  - Mostarica-Stojković, Marija B.
AU  - Stošić-Grujičić, Stanislava
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1141
AB  - To determine the mechanism underlying ribavirin induced amelioration of experimental autoimmune encephalomyelitis (EAE), cytokine profiles were evaluated in draining lymph node (DLN) cell culture supernatants and spinal cord obtained from EAE and/or ribavirin-treated EAE Dark Agouti rats. Administration of ribavirin to EAE rats markedly affected the production of pro-inflammatory cytokines IFN-gamma, IL-1 beta and TNF-alpha in DLN and spinal cord, thus shifting the balance towards the anti-inflammatory cytokines IL-10 and TGF-beta. These findings suggest that ribavirin attenuates EAE by limiting cytokine-mediated immunoinflammatory events leading to CNS destruction. The conducted experiments provide rationale for ribavirin to be considered as a candidate drug in the development of new therapeutic strategies for the treatment of autoimmune diseases in humans, such as multiple sclerosis. (c) 2008 Elsevier B.V. All rights reserved.
PB  - Amsterdam, Netherlands: Elsevier
T2  - International Immunopharmacology
T1  - Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production
IS  - 9
VL  - 8
DO  - 10.1016/j.intimp.2008.05.008
SP  - 1282
EP  - 1290
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1141
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Bjelobaba, Ivana and Peković, Sanja and Dacić, Sanja and Nedeljković, Nadežda N. and Mostarica-Stojković, Marija B. and Stošić-Grujičić, Stanislava and Rakić, Ljubisav and Stojiljković, Mirjana B.",
year = "2008",
abstract = "To determine the mechanism underlying ribavirin induced amelioration of experimental autoimmune encephalomyelitis (EAE), cytokine profiles were evaluated in draining lymph node (DLN) cell culture supernatants and spinal cord obtained from EAE and/or ribavirin-treated EAE Dark Agouti rats. Administration of ribavirin to EAE rats markedly affected the production of pro-inflammatory cytokines IFN-gamma, IL-1 beta and TNF-alpha in DLN and spinal cord, thus shifting the balance towards the anti-inflammatory cytokines IL-10 and TGF-beta. These findings suggest that ribavirin attenuates EAE by limiting cytokine-mediated immunoinflammatory events leading to CNS destruction. The conducted experiments provide rationale for ribavirin to be considered as a candidate drug in the development of new therapeutic strategies for the treatment of autoimmune diseases in humans, such as multiple sclerosis. (c) 2008 Elsevier B.V. All rights reserved.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "International Immunopharmacology",
title = "Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production",
number = "9",
volume = "8",
doi = "10.1016/j.intimp.2008.05.008",
pages = "1282-1290",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1141"
}

Lavrnja, I., Savić, D., Bjelobaba, I., Peković, S., Dacić, S., Nedeljković, N. N., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Rakić, L.,& Stojiljković, M. B.. (2008). Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production. in International Immunopharmacology
Amsterdam, Netherlands: Elsevier., 8(9), 1282-1290.
https://doi.org/10.1016/j.intimp.2008.05.008
https://hdl.handle.net/21.15107/rcub_ibiss_1141

Lavrnja I, Savić D, Bjelobaba I, Peković S, Dacić S, Nedeljković NN, Mostarica-Stojković MB, Stošić-Grujičić S, Rakić L, Stojiljković MB. Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production. in International Immunopharmacology. 2008;8(9):1282-1290.
doi:10.1016/j.intimp.2008.05.008
https://hdl.handle.net/21.15107/rcub_ibiss_1141 .

Lavrnja, Irena, Savić, Danijela, Bjelobaba, Ivana, Peković, Sanja, Dacić, Sanja, Nedeljković, Nadežda N., Mostarica-Stojković, Marija B., Stošić-Grujičić, Stanislava, Rakić, Ljubisav, Stojiljković, Mirjana B., "Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production" in International Immunopharmacology, 8, no. 9 (2008):1282-1290,
https://doi.org/10.1016/j.intimp.2008.05.008 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1141 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Rakić, Ljubisav
AU  - Nedeljković, Nadežda N.
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1579
AB  - Distribution of two enzymes involved in the ectonucleotidase enzyme chain, ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and ecto-5'-nucleotidase, was assessed by immunohistochemistry in the rat hippocampus. Obtained results have shown co-expression of the enzymes in the hippocampal region, as well as wide and strikingly similar cellular distribution. Both enzymes were expressed at the surface of pyramidal neurons in the CA1 and CA2 sections, while cells in the CA3 section were faintly stained. The granule cell layer of the dentate gyrus was moderately stained for NTPDase1, as well as for ecto-5'-nucleotidase. Glial association for ecto-5'-nucleotidase was also observed, and fiber tracts were intensively stained for both enzymes. This is the first comparative study of NTPDase1 and ecto-5'-nucleotidase distribution in the rat hippocampus. Obtained results suggest that the broad overlapping distribution of these enzymes in neurons and glial cells reflects the functional importance of ectonucleotidase actions in the nervous system.
PB  - New York, NY: Springer
T2  - Cellular and Molecular Neurobiology
T1  - Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution
IS  - 6
VL  - 27
DO  - 10.1007/s10571-007-9159-8
SP  - 731
EP  - 743
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1579
ER  - 

@article{
author = "Bjelobaba, Ivana and Stojiljković, Mirjana B and Peković, Sanja and Dacić, Sanja and Lavrnja, Irena and Savić, Danijela and Rakić, Ljubisav and Nedeljković, Nadežda N.",
year = "2007",
abstract = "Distribution of two enzymes involved in the ectonucleotidase enzyme chain, ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and ecto-5'-nucleotidase, was assessed by immunohistochemistry in the rat hippocampus. Obtained results have shown co-expression of the enzymes in the hippocampal region, as well as wide and strikingly similar cellular distribution. Both enzymes were expressed at the surface of pyramidal neurons in the CA1 and CA2 sections, while cells in the CA3 section were faintly stained. The granule cell layer of the dentate gyrus was moderately stained for NTPDase1, as well as for ecto-5'-nucleotidase. Glial association for ecto-5'-nucleotidase was also observed, and fiber tracts were intensively stained for both enzymes. This is the first comparative study of NTPDase1 and ecto-5'-nucleotidase distribution in the rat hippocampus. Obtained results suggest that the broad overlapping distribution of these enzymes in neurons and glial cells reflects the functional importance of ectonucleotidase actions in the nervous system.",
publisher = "New York, NY: Springer",
journal = "Cellular and Molecular Neurobiology",
title = "Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution",
number = "6",
volume = "27",
doi = "10.1007/s10571-007-9159-8",
pages = "731-743",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1579"
}

Bjelobaba, I., Stojiljković, M. B., Peković, S., Dacić, S., Lavrnja, I., Savić, D., Rakić, L.,& Nedeljković, N. N.. (2007). Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution. in Cellular and Molecular Neurobiology
New York, NY: Springer., 27(6), 731-743.
https://doi.org/10.1007/s10571-007-9159-8
https://hdl.handle.net/21.15107/rcub_ibiss_1579

Bjelobaba I, Stojiljković MB, Peković S, Dacić S, Lavrnja I, Savić D, Rakić L, Nedeljković NN. Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution. in Cellular and Molecular Neurobiology. 2007;27(6):731-743.
doi:10.1007/s10571-007-9159-8
https://hdl.handle.net/21.15107/rcub_ibiss_1579 .

Bjelobaba, Ivana, Stojiljković, Mirjana B, Peković, Sanja, Dacić, Sanja, Lavrnja, Irena, Savić, Danijela, Rakić, Ljubisav, Nedeljković, Nadežda N., "Immunohistological determination of ecto-nucleoside triphosphate diphosphohydrolase1 (NTPDase1) and 5'-nucleotidase in rat hippocampus reveals overlapping distribution" in Cellular and Molecular Neurobiology, 27, no. 6 (2007):731-743,
https://doi.org/10.1007/s10571-007-9159-8 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1579 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadežda N.
AU  - Šubašić, Sanja A
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Savić, Danijela
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1627
AB  - Immunohistochemical study was performed to determine distribution of ecto-nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) in adult rat forebrain. The study revealed widespread distribution of NPP1 in rat forebrain, yet with regional differences in the expression pattern and abundance. Strong NPP1 immunoreaction was detected in pyramidal cell layer of cerebral cortex and hippocampus, and in the midline regions of hypothalamus and thalamus. In many immunopositive forebrain areas, NPP1 was mainly localized at neuronal cell bodies. However, prominent immunoreaction was also detected at ependymal cells, tanycytes, endothelial cells of the capillaries and cells of the choroid plexus, suggesting that NPP1 could be involved in some highly specialized transport process.
PB  - Elsevier
T2  - Brain Research
T1  - Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain
IS  - 1
VL  - 1120
DO  - 10.1016/j.brainres.2006.08.114
SP  - 54
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1627
ER  - 

@article{
author = "Bjelobaba, Ivana and Nedeljković, Nadežda N. and Šubašić, Sanja A and Lavrnja, Irena and Peković, Sanja and Savić, Danijela and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2006",
abstract = "Immunohistochemical study was performed to determine distribution of ecto-nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) in adult rat forebrain. The study revealed widespread distribution of NPP1 in rat forebrain, yet with regional differences in the expression pattern and abundance. Strong NPP1 immunoreaction was detected in pyramidal cell layer of cerebral cortex and hippocampus, and in the midline regions of hypothalamus and thalamus. In many immunopositive forebrain areas, NPP1 was mainly localized at neuronal cell bodies. However, prominent immunoreaction was also detected at ependymal cells, tanycytes, endothelial cells of the capillaries and cells of the choroid plexus, suggesting that NPP1 could be involved in some highly specialized transport process.",
publisher = "Elsevier",
journal = "Brain Research",
title = "Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain",
number = "1",
volume = "1120",
doi = "10.1016/j.brainres.2006.08.114",
pages = "54-63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1627"
}

Bjelobaba, I., Nedeljković, N. N., Šubašić, S. A., Lavrnja, I., Peković, S., Savić, D., Rakić, L.,& Stojiljković, M. B.. (2006). Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain. in Brain Research
Elsevier., 1120(1), 54-63.
https://doi.org/10.1016/j.brainres.2006.08.114
https://hdl.handle.net/21.15107/rcub_ibiss_1627

Bjelobaba I, Nedeljković NN, Šubašić SA, Lavrnja I, Peković S, Savić D, Rakić L, Stojiljković MB. Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain. in Brain Research. 2006;1120(1):54-63.
doi:10.1016/j.brainres.2006.08.114
https://hdl.handle.net/21.15107/rcub_ibiss_1627 .

Bjelobaba, Ivana, Nedeljković, Nadežda N., Šubašić, Sanja A, Lavrnja, Irena, Peković, Sanja, Savić, Danijela, Rakić, Ljubisav, Stojiljković, Mirjana B, "Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain" in Brain Research, 1120, no. 1 (2006):54-63,
https://doi.org/10.1016/j.brainres.2006.08.114 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1627 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Peković, Sanja
AU  - Šubašić, Sanja A
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Nedeljković, Nadežda N
AU  - Medić-Mijačević, Ljubica
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1714
AB  - The effect of combined treatment with ribavirin and tiazofurin on the development of experimental autoimmune encephalomyelitis, the best characterized animal model for human autoimmune disease multiple sclerosis, was investigated. The disease was induced in highly susceptible Dark Agouti rats with spinal cord homogenate in complete Freund's adjuvant. Although ribavirin or tiazofurin alone reduced the clinical and histopathological signs of experimental autoinmume encephalomyelitis, the combination of drugs achieved the same effect with significantly lower doses.
T2  - Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1).
T1  - Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis
DO  - 10.1196/annals.1342.047
SP  - 392
EP  - 395
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1714
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Peković, Sanja and Šubašić, Sanja A and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Nedeljković, Nadežda N and Medić-Mijačević, Ljubica and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2005",
abstract = "The effect of combined treatment with ribavirin and tiazofurin on the development of experimental autoimmune encephalomyelitis, the best characterized animal model for human autoimmune disease multiple sclerosis, was investigated. The disease was induced in highly susceptible Dark Agouti rats with spinal cord homogenate in complete Freund's adjuvant. Although ribavirin or tiazofurin alone reduced the clinical and histopathological signs of experimental autoinmume encephalomyelitis, the combination of drugs achieved the same effect with significantly lower doses.",
journal = "Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1).",
title = "Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis",
doi = "10.1196/annals.1342.047",
pages = "392-395",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1714"
}

Lavrnja, I., Savić, D., Peković, S., Šubašić, S. A., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Nedeljković, N. N., Medić-Mijačević, L., Rakić, L.,& Stojiljković, M.. (2005). Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis. in Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1)., 392-395.
https://doi.org/10.1196/annals.1342.047
https://hdl.handle.net/21.15107/rcub_ibiss_1714

Lavrnja I, Savić D, Peković S, Šubašić SA, Mostarica-Stojković MB, Stošić-Grujičić S, Nedeljković NN, Medić-Mijačević L, Rakić L, Stojiljković M. Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis. in Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1).. 2005;:392-395.
doi:10.1196/annals.1342.047
https://hdl.handle.net/21.15107/rcub_ibiss_1714 .

Lavrnja, Irena, Savić, Danijela, Peković, Sanja, Šubašić, Sanja A, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Nedeljković, Nadežda N, Medić-Mijačević, Ljubica, Rakić, Ljubisav, Stojiljković, Mirjana, "Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis" in Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1). (2005):392-395,
https://doi.org/10.1196/annals.1342.047 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1714 .

TY  - JOUR
AU  - Milenković, Ivanka M.
AU  - Filipović, R
AU  - Nedeljković, Nadezda N
AU  - Peković, Sanja
AU  - Ćulić, Milka
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1731
AB  - 1. Spatio-temporal changes in phosphorylated (pNFP) and nonphosphorylated (npNFP) neurofilament proteins were assessed immunocytochemicaly in adult rat cerebellum, 2 - 30 days following unilateral injection of kainic acid (KA) or physiological saline (SC). 2. Analysis of the staining intensity and pattern demonstrated that injection of both KA and physiological saline elicited significant and long-lasting increase of pNFP and npNFP immunoreactivity, at the ipsilateral, and to lesser extent at the contralateral side of lesion. 3. Kainate intoxication induced abundant expression of pNFP and npNFP in cerebellar white matter, as well as in all layers of perilesioned cortex. Higher pNFP expression was evidenced in the Purkinje cell layer, particularly at cell bodies, initial segments, and proximal dendrites, which normally do not contain pNFP. In addition, synaptophysin immunocytochemistry was used as a marker of synaptogenesis and plasticity. 4. Spatio-temporal pattern of NFP and synaptophysin expression suggests that perilesioned cortex undergoes dynamic changes following brain demage and possess a reparative capacity to abridge the consequences of brain trauma.
T2  - Cellular and Molecular Neurobiology
T1  - Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats
IS  - 3
VL  - 24
EP  - 378
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1731
ER  - 

@article{
author = "Milenković, Ivanka M. and Filipović, R and Nedeljković, Nadezda N and Peković, Sanja and Ćulić, Milka and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2004",
abstract = "1. Spatio-temporal changes in phosphorylated (pNFP) and nonphosphorylated (npNFP) neurofilament proteins were assessed immunocytochemicaly in adult rat cerebellum, 2 - 30 days following unilateral injection of kainic acid (KA) or physiological saline (SC). 2. Analysis of the staining intensity and pattern demonstrated that injection of both KA and physiological saline elicited significant and long-lasting increase of pNFP and npNFP immunoreactivity, at the ipsilateral, and to lesser extent at the contralateral side of lesion. 3. Kainate intoxication induced abundant expression of pNFP and npNFP in cerebellar white matter, as well as in all layers of perilesioned cortex. Higher pNFP expression was evidenced in the Purkinje cell layer, particularly at cell bodies, initial segments, and proximal dendrites, which normally do not contain pNFP. In addition, synaptophysin immunocytochemistry was used as a marker of synaptogenesis and plasticity. 4. Spatio-temporal pattern of NFP and synaptophysin expression suggests that perilesioned cortex undergoes dynamic changes following brain demage and possess a reparative capacity to abridge the consequences of brain trauma.",
journal = "Cellular and Molecular Neurobiology",
title = "Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats",
number = "3",
volume = "24",
pages = "378",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1731"
}

Milenković, I. M., Filipović, R., Nedeljković, N. N., Peković, S., Ćulić, M., Rakić, L.,& Stojiljković, M. B.. (2004). Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats. in Cellular and Molecular Neurobiology, 24(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1731

Milenković IM, Filipović R, Nedeljković NN, Peković S, Ćulić M, Rakić L, Stojiljković MB. Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats. in Cellular and Molecular Neurobiology. 2004;24(3):null-378.
https://hdl.handle.net/21.15107/rcub_ibiss_1731 .

Milenković, Ivanka M., Filipović, R, Nedeljković, Nadezda N, Peković, Sanja, Ćulić, Milka, Rakić, Ljubisav, Stojiljković, Mirjana B, "Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats" in Cellular and Molecular Neurobiology, 24, no. 3 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1731 .