TY  - CONF
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Mišić, Danijela
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Jovanović, Olivera
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2023
UR  - https://europlantbiology2023.org/wp-content/uploads/2023/07/PBE2023-Abstract-Book.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6224
AB  - Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6224
ER  - 

@conference{
author = "Živković, Suzana and Milutinović, Milica and Mišić, Danijela and Nakarada, Đura and Mojović, Miloš and Jovanović, Olivera and Škoro, Nikola and Puač, Nevena",
year = "2023",
abstract = "Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6224"
}

Živković, S., Milutinović, M., Mišić, D., Nakarada, Đ., Mojović, M., Jovanović, O., Škoro, N.,& Puač, N.. (2023). Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224

Živković S, Milutinović M, Mišić D, Nakarada Đ, Mojović M, Jovanović O, Škoro N, Puač N. Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

Živković, Suzana, Milutinović, Milica, Mišić, Danijela, Nakarada, Đura, Mojović, Miloš, Jovanović, Olivera, Škoro, Nikola, Puač, Nevena, "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):154,
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5505
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020346
SP  - 346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
number = "2",
volume = "12",
doi = "10.3390/antiox12020346",
pages = "346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
Basel: MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić U, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš, Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - CONF
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Devrnja, Nina
AU  - Banjanac, Tijana
AU  - Todorović, Miloš
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5270
AB  - Identifying plant species tolerant to hypoxia and determining mechanisms this tolerance is standing upon may aid in improving nutritional quality of fruits of waterlogging-intolerant crops. Berries of Solanum dulcamara L. (bittersweet), a Eurasian species belonging to the Solanaceae family that grows in both dry habitats and wetlands, represent a suitable hypoxia-tolerant model species to study redox processes in relation to fruit development. Using biochemical and physicochemical analyses, we examined differences in enzymatic and non-enzymatic components of the antioxidant system of S. dulcamara during the transition from mature green (MG) to ripe red (RR) developmental stage. By combining untargeted (UHPLC/Orbitrap MSn) and targeted (UHPLC-DAD MS2) metabolomics approaches, we revealed a significant change in polyphenolic profile of S. dulcamara fruits during fruit ripening. In order to improve knowledge on the link between metabolism and cell redox status, Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) of reactive oxygen species (ROS) in intact fruits, fruit sections and extracts, were performed in parallel with the analysis of tissue-specific distribution of antioxidants and activity of antioxidant enzymes, which demonstrated alterations in the redox state and the increase of total antioxidant capacity of bittersweet berries during ripening, especially of outer layers (pericarp) of the fruits. This knowledge could provide important information to direct research on fruit quality improvement of flood-intolerant crops that are a close relatives of bittersweet, such as tomato and eggplant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5270
ER  - 

@conference{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Gašić, Uroš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Devrnja, Nina and Banjanac, Tijana and Todorović, Miloš and Mojović, Miloš and Mišić, Danijela",
year = "2022",
abstract = "Identifying plant species tolerant to hypoxia and determining mechanisms this tolerance is standing upon may aid in improving nutritional quality of fruits of waterlogging-intolerant crops. Berries of Solanum dulcamara L. (bittersweet), a Eurasian species belonging to the Solanaceae family that grows in both dry habitats and wetlands, represent a suitable hypoxia-tolerant model species to study redox processes in relation to fruit development. Using biochemical and physicochemical analyses, we examined differences in enzymatic and non-enzymatic components of the antioxidant system of S. dulcamara during the transition from mature green (MG) to ripe red (RR) developmental stage. By combining untargeted (UHPLC/Orbitrap MSn) and targeted (UHPLC-DAD MS2) metabolomics approaches, we revealed a significant change in polyphenolic profile of S. dulcamara fruits during fruit ripening. In order to improve knowledge on the link between metabolism and cell redox status, Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) of reactive oxygen species (ROS) in intact fruits, fruit sections and extracts, were performed in parallel with the analysis of tissue-specific distribution of antioxidants and activity of antioxidant enzymes, which demonstrated alterations in the redox state and the increase of total antioxidant capacity of bittersweet berries during ripening, especially of outer layers (pericarp) of the fruits. This knowledge could provide important information to direct research on fruit quality improvement of flood-intolerant crops that are a close relatives of bittersweet, such as tomato and eggplant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations",
pages = "126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5270"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Gašić, U., Živković, S., Savić, J., Skorić, M., Devrnja, N., Banjanac, T., Todorović, M., Mojović, M.,& Mišić, D.. (2022). Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 126.
https://hdl.handle.net/21.15107/rcub_ibiss_5270

Milutinović M, Nakarada Đ, Božunović J, Gašić U, Živković S, Savić J, Skorić M, Devrnja N, Banjanac T, Todorović M, Mojović M, Mišić D. Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:126.
https://hdl.handle.net/21.15107/rcub_ibiss_5270 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Gašić, Uroš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Devrnja, Nina, Banjanac, Tijana, Todorović, Miloš, Mojović, Miloš, Mišić, Danijela, "Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):126,
https://hdl.handle.net/21.15107/rcub_ibiss_5270 .

TY  - JOUR
AU  - Krunić, Matija
AU  - Ristić, Biljana
AU  - Bošnjak, Mihajlo
AU  - Paunović, Verica
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Mirčić, Aleksandar
AU  - Marković, Zoran
AU  - Todorović-Marković, Biljana
AU  - Jovanović, Svetlana
AU  - Kleut, Duška
AU  - Mojović, Miloš
AU  - Nakarada, Đura
AU  - Marković, Olivera
AU  - Vuković, Irena
AU  - Harhaji-Trajković, Ljubica
AU  - Trajković, Vladimir
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0891584921007760
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4655
AB  - We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•-), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy.
PB  - Elsevier Inc.
T2  - Free Radical Biology and Medicine
T1  - Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.10.025
SP  - 167
EP  - 180
ER  - 

@article{
author = "Krunić, Matija and Ristić, Biljana and Bošnjak, Mihajlo and Paunović, Verica and Tovilović-Kovačević, Gordana and Zogović, Nevena and Mirčić, Aleksandar and Marković, Zoran and Todorović-Marković, Biljana and Jovanović, Svetlana and Kleut, Duška and Mojović, Miloš and Nakarada, Đura and Marković, Olivera and Vuković, Irena and Harhaji-Trajković, Ljubica and Trajković, Vladimir",
year = "2021",
abstract = "We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•-), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy.",
publisher = "Elsevier Inc.",
journal = "Free Radical Biology and Medicine",
title = "Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.10.025",
pages = "167-180"
}

Krunić, M., Ristić, B., Bošnjak, M., Paunović, V., Tovilović-Kovačević, G., Zogović, N., Mirčić, A., Marković, Z., Todorović-Marković, B., Jovanović, S., Kleut, D., Mojović, M., Nakarada, Đ., Marković, O., Vuković, I., Harhaji-Trajković, L.,& Trajković, V.. (2021). Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.. in Free Radical Biology and Medicine
Elsevier Inc.., 177, 167-180.
https://doi.org/10.1016/j.freeradbiomed.2021.10.025

Krunić M, Ristić B, Bošnjak M, Paunović V, Tovilović-Kovačević G, Zogović N, Mirčić A, Marković Z, Todorović-Marković B, Jovanović S, Kleut D, Mojović M, Nakarada Đ, Marković O, Vuković I, Harhaji-Trajković L, Trajković V. Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death.. in Free Radical Biology and Medicine. 2021;177:167-180.
doi:10.1016/j.freeradbiomed.2021.10.025 .

Krunić, Matija, Ristić, Biljana, Bošnjak, Mihajlo, Paunović, Verica, Tovilović-Kovačević, Gordana, Zogović, Nevena, Mirčić, Aleksandar, Marković, Zoran, Todorović-Marković, Biljana, Jovanović, Svetlana, Kleut, Duška, Mojović, Miloš, Nakarada, Đura, Marković, Olivera, Vuković, Irena, Harhaji-Trajković, Ljubica, Trajković, Vladimir, "Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death." in Free Radical Biology and Medicine, 177 (2021):167-180,
https://doi.org/10.1016/j.freeradbiomed.2021.10.025 . .

TY  - JOUR
AU  - Vesković, Ana
AU  - Nakarada, Đura
AU  - Pavićević, Aleksandra
AU  - Prokić, Bogomir
AU  - Perović, Milka
AU  - Kanazir, Selma
AU  - Popović-Bijelić, Ana
AU  - Mojović, Miloš
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4221
AB  - BACKGROUND Alzheimer's disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the re- search focus from Aβ to other pathological features of the disease. OBJECTIVE The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. METHOD In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibit- ing different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectros- copy was employed to investigate the brain metal content. RESULTS This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-me- diated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. CONCLUSION The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neu- ronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spec- troscopy application in preclinical studies of neurodegenerative diseases.
PB  - Bentham Science Publishers Ltd.
T2  - Current Alzheimer Research
T1  - In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.
IS  - 1
VL  - 18
DO  - 10.2174/1567205018666210324121156
SP  - 25
EP  - 34
ER  - 

@article{
author = "Vesković, Ana and Nakarada, Đura and Pavićević, Aleksandra and Prokić, Bogomir and Perović, Milka and Kanazir, Selma and Popović-Bijelić, Ana and Mojović, Miloš",
year = "2021",
abstract = "BACKGROUND Alzheimer's disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the re- search focus from Aβ to other pathological features of the disease. OBJECTIVE The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. METHOD In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibit- ing different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectros- copy was employed to investigate the brain metal content. RESULTS This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-me- diated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. CONCLUSION The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neu- ronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spec- troscopy application in preclinical studies of neurodegenerative diseases.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Alzheimer Research",
title = "In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.",
number = "1",
volume = "18",
doi = "10.2174/1567205018666210324121156",
pages = "25-34"
}

Vesković, A., Nakarada, Đ., Pavićević, A., Prokić, B., Perović, M., Kanazir, S., Popović-Bijelić, A.,& Mojović, M.. (2021). In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.. in Current Alzheimer Research
Bentham Science Publishers Ltd.., 18(1), 25-34.
https://doi.org/10.2174/1567205018666210324121156

Vesković A, Nakarada Đ, Pavićević A, Prokić B, Perović M, Kanazir S, Popović-Bijelić A, Mojović M. In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease.. in Current Alzheimer Research. 2021;18(1):25-34.
doi:10.2174/1567205018666210324121156 .

Vesković, Ana, Nakarada, Đura, Pavićević, Aleksandra, Prokić, Bogomir, Perović, Milka, Kanazir, Selma, Popović-Bijelić, Ana, Mojović, Miloš, "In vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood--Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease." in Current Alzheimer Research, 18, no. 1 (2021):25-34,
https://doi.org/10.2174/1567205018666210324121156 . .

TY  - JOUR
AU  - Vujačić-Mirski, Ksenija
AU  - Bruns, Kai
AU  - Kalinović, Sanela
AU  - Oelze, Matthias
AU  - Kröller-Schön, Swenja
AU  - Steven, Sebastian
AU  - Mojović, Miloš
AU  - Korać, Bato
AU  - Münzel, Thomas
AU  - Daiber, Andreas
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/5/388
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32384768
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3672
AB  - Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2•-) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected.
T2  - Antioxidants (Basel, Switzerland)
T2  - Antioxidants (Basel, Switzerland)
T1  - Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods.
IS  - 5
VL  - 9
DO  - 10.3390/antiox9050388
SP  - 388
ER  - 

@article{
author = "Vujačić-Mirski, Ksenija and Bruns, Kai and Kalinović, Sanela and Oelze, Matthias and Kröller-Schön, Swenja and Steven, Sebastian and Mojović, Miloš and Korać, Bato and Münzel, Thomas and Daiber, Andreas",
year = "2020",
abstract = "Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2•-) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected.",
journal = "Antioxidants (Basel, Switzerland), Antioxidants (Basel, Switzerland)",
title = "Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods.",
number = "5",
volume = "9",
doi = "10.3390/antiox9050388",
pages = "388"
}

Vujačić-Mirski, K., Bruns, K., Kalinović, S., Oelze, M., Kröller-Schön, S., Steven, S., Mojović, M., Korać, B., Münzel, T.,& Daiber, A.. (2020). Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods.. in Antioxidants (Basel, Switzerland), 9(5), 388.
https://doi.org/10.3390/antiox9050388

Vujačić-Mirski K, Bruns K, Kalinović S, Oelze M, Kröller-Schön S, Steven S, Mojović M, Korać B, Münzel T, Daiber A. Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods.. in Antioxidants (Basel, Switzerland). 2020;9(5):388.
doi:10.3390/antiox9050388 .

Vujačić-Mirski, Ksenija, Bruns, Kai, Kalinović, Sanela, Oelze, Matthias, Kröller-Schön, Swenja, Steven, Sebastian, Mojović, Miloš, Korać, Bato, Münzel, Thomas, Daiber, Andreas, "Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods." in Antioxidants (Basel, Switzerland), 9, no. 5 (2020):388,
https://doi.org/10.3390/antiox9050388 . .

TY  - JOUR
AU  - Egea, Javier
AU  - Fabregat, Isabel
AU  - Frapart, Yves M.
AU  - Ghezzi, Pietro
AU  - Görlach, Agnes
AU  - Kietzmann, Thomas
AU  - Kubaichuk, Kateryna
AU  - Knaus, Ulla G.
AU  - Lopez, Manuela G.
AU  - Olaso-Gonzalez, Gloria
AU  - Petry, Andreas
AU  - Schulz, Rainer
AU  - Vina, Jose
AU  - Winyard, Paul
AU  - Abbas, Kahina
AU  - Ademowo, Opeyemi S.
AU  - Afonso, Catarina B.
AU  - Andreadou, Ioanna
AU  - Antelmann, Haike
AU  - Antunes, Fernando
AU  - Aslan, Mutay
AU  - Bachschmid, Markus M.
AU  - Barbosa, Rui M.
AU  - Belousov, Vsevolod
AU  - Berndt, Carsten
AU  - Bernlohr, David
AU  - Bertrán, Esther
AU  - Bindoli, Alberto
AU  - Bottari, Serge P.
AU  - Brito, Paula M.
AU  - Carrara, Guia
AU  - Casas, Ana I.
AU  - Chatzi, Afroditi
AU  - Chondrogianni, Niki
AU  - Conrad, Marcus
AU  - Cooke, Marcus S.
AU  - Costa, João G.
AU  - Cuadrado, Antonio
AU  - My-Chan Dang, Pham
AU  - De Smet, Barbara
AU  - Debelec-Butuner, Bilge
AU  - Dias, Irundika H.K.
AU  - Dunn, Joe Dan
AU  - Edson, Amanda J.
AU  - El Assar, Mariam
AU  - El-Benna, Jamel
AU  - Ferdinandy, Péter
AU  - Fernandes, Ana S.
AU  - Fladmark, Kari E.
AU  - Förstermann, Ulrich
AU  - Giniatullin, Rashid
AU  - Giricz, Zoltán
AU  - Görbe, Anikó
AU  - Griffiths, Helen
AU  - Hampl, Vaclav
AU  - Hanf, Alina
AU  - Herget, Jan
AU  - Hernansanz-Agustín, Pablo
AU  - Hillion, Melanie
AU  - Huang, Jingjing
AU  - Ilikay, Serap
AU  - Jansen-Dürr, Pidder
AU  - Jaquet, Vincent
AU  - Joles, Jaap A.
AU  - Kalyanaraman, Balaraman
AU  - Kaminskyy, Danylo
AU  - Karbaschi, Mahsa
AU  - Kleanthous, Marina
AU  - Klotz, Lars-Oliver
AU  - Korać, Bato
AU  - Korkmaz, Kemal Sami
AU  - Koziel, Rafal
AU  - Kračun, Damir
AU  - Krause, Karl-Heinz
AU  - Křen, Vladimír
AU  - Krieg, Thomas
AU  - Laranjinha, João
AU  - Lazou, Antigone
AU  - Li, Huige
AU  - Martínez-Ruiz, Antonio
AU  - Matsui, Reiko
AU  - McBean, Gethin J.
AU  - Meredith, Stuart P.
AU  - Messens, Joris
AU  - Miguel, Verónica
AU  - Mikhed, Yuliya
AU  - Milisav, Irina
AU  - Milković, Lidija
AU  - Miranda-Vizuete, Antonio
AU  - Mojović, Miloš
AU  - Monsalve, María
AU  - Mouthuy, Pierre-Alexis
AU  - Mulvey, John
AU  - Münzel, Thomas
AU  - Muzykantov, Vladimir
AU  - Nguyen, Isabel T.N.
AU  - Oelze, Matthias
AU  - Oliveira, Nuno G.
AU  - Palmeira, Carlos M.
AU  - Papaevgeniou, Nikoletta
AU  - Pavićević, Aleksandra
AU  - Pedre, Brandán
AU  - Peyrot, Fabienne
AU  - Phylactides, Marios
AU  - Pircalabioru, Gratiela G.
AU  - Pitt, Andrew R.
AU  - Poulsen, Henrik E.
AU  - Prieto, Ignacio
AU  - Rigobello, Maria Pia
AU  - Robledinos-Antón, Natalia
AU  - Rodríguez-Mañas, Leocadio
AU  - Rolo, Anabela P.
AU  - Rousset, Francis
AU  - Ruskovska, Tatjana
AU  - Saraiva, Nuno
AU  - Sasson, Shlomo
AU  - Schröder, Katrin
AU  - Semen, Khrystyna
AU  - Seredenina, Tamara
AU  - Shakirzyanova, Anastasia
AU  - Smith, Geoffrey L.
AU  - Soldati, Thierry
AU  - Sousa, Bebiana C.
AU  - Spickett, Corinne M.
AU  - Stančić, Ana
AU  - Stasia, Marie José
AU  - Steinbrenner, Holger
AU  - Stepanić, Višnja
AU  - Steven, Sebastian
AU  - Tokatlidis, Kostas
AU  - Tuncay, Erkan
AU  - Turan, Belma
AU  - Ursini, Fulvio
AU  - Vacek, Jan
AU  - Vajnerova, Olga
AU  - Valentová, Kateřina
AU  - Van Breusegem, Frank
AU  - Varisli, Lokman
AU  - Veal, Elizabeth A.
AU  - Yalçın, A. Suha
AU  - Yelisyeyeva, Olha
AU  - Žarković, Neven
AU  - Zatloukalová, Martina
AU  - Zielonka, Jacek
AU  - Touyz, Rhian M.
AU  - Papapetropoulos, Andreas
AU  - Grune, Tilman
AU  - Lamas, Santiago
AU  - Schmidt, Harald H.H.W.
AU  - Di Lisa, Fabio
AU  - Daiber, Andreas
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S2213231717303373
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2778
AB  - The European Cooperation in Science and Technology (COST) provides an ideal framework to establish multi-disciplinary research networks. COST Action BM1203 (EU-ROS) represents a consortium of researchers from different disciplines who are dedicated to providing new insights and tools for better understanding redox biology and medicine and, in the long run, to finding new therapeutic strategies to target dysregulated redox processes in various diseases. This report highlights the major achievements of EU-ROS as well as research updates and new perspectives arising from its members. The EU-ROS consortium comprised more than 140 active members who worked together for four years on the topics briefly described below. The formation of reactive oxygen and nitrogen species (RONS) is an established hallmark of our aerobic environment and metabolism but RONS also act as messengers via redox regulation of essential cellular processes. The fact that many diseases have been found to be associated with oxidative stress established the theory of oxidative stress as a trigger of diseases that can be corrected by antioxidant therapy. However, while experimental studies support this thesis, clinical studies still generate controversial results, due to complex pathophysiology of oxidative stress in humans. For future improvement of antioxidant therapy and better understanding of redox-associated disease progression detailed knowledge on the sources and targets of RONS formation and discrimination of their detrimental or beneficial roles is required. In order to advance this important area of biology and medicine, highly synergistic approaches combining a variety of diverse and contrasting disciplines are needed.
T2  - Redox Biology
T1  - European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)
VL  - 13
DO  - 10.1016/j.redox.2017.05.007
SP  - 94
EP  - 162
ER  - 

@article{
author = "Egea, Javier and Fabregat, Isabel and Frapart, Yves M. and Ghezzi, Pietro and Görlach, Agnes and Kietzmann, Thomas and Kubaichuk, Kateryna and Knaus, Ulla G. and Lopez, Manuela G. and Olaso-Gonzalez, Gloria and Petry, Andreas and Schulz, Rainer and Vina, Jose and Winyard, Paul and Abbas, Kahina and Ademowo, Opeyemi S. and Afonso, Catarina B. and Andreadou, Ioanna and Antelmann, Haike and Antunes, Fernando and Aslan, Mutay and Bachschmid, Markus M. and Barbosa, Rui M. and Belousov, Vsevolod and Berndt, Carsten and Bernlohr, David and Bertrán, Esther and Bindoli, Alberto and Bottari, Serge P. and Brito, Paula M. and Carrara, Guia and Casas, Ana I. and Chatzi, Afroditi and Chondrogianni, Niki and Conrad, Marcus and Cooke, Marcus S. and Costa, João G. and Cuadrado, Antonio and My-Chan Dang, Pham and De Smet, Barbara and Debelec-Butuner, Bilge and Dias, Irundika H.K. and Dunn, Joe Dan and Edson, Amanda J. and El Assar, Mariam and El-Benna, Jamel and Ferdinandy, Péter and Fernandes, Ana S. and Fladmark, Kari E. and Förstermann, Ulrich and Giniatullin, Rashid and Giricz, Zoltán and Görbe, Anikó and Griffiths, Helen and Hampl, Vaclav and Hanf, Alina and Herget, Jan and Hernansanz-Agustín, Pablo and Hillion, Melanie and Huang, Jingjing and Ilikay, Serap and Jansen-Dürr, Pidder and Jaquet, Vincent and Joles, Jaap A. and Kalyanaraman, Balaraman and Kaminskyy, Danylo and Karbaschi, Mahsa and Kleanthous, Marina and Klotz, Lars-Oliver and Korać, Bato and Korkmaz, Kemal Sami and Koziel, Rafal and Kračun, Damir and Krause, Karl-Heinz and Křen, Vladimír and Krieg, Thomas and Laranjinha, João and Lazou, Antigone and Li, Huige and Martínez-Ruiz, Antonio and Matsui, Reiko and McBean, Gethin J. and Meredith, Stuart P. and Messens, Joris and Miguel, Verónica and Mikhed, Yuliya and Milisav, Irina and Milković, Lidija and Miranda-Vizuete, Antonio and Mojović, Miloš and Monsalve, María and Mouthuy, Pierre-Alexis and Mulvey, John and Münzel, Thomas and Muzykantov, Vladimir and Nguyen, Isabel T.N. and Oelze, Matthias and Oliveira, Nuno G. and Palmeira, Carlos M. and Papaevgeniou, Nikoletta and Pavićević, Aleksandra and Pedre, Brandán and Peyrot, Fabienne and Phylactides, Marios and Pircalabioru, Gratiela G. and Pitt, Andrew R. and Poulsen, Henrik E. and Prieto, Ignacio and Rigobello, Maria Pia and Robledinos-Antón, Natalia and Rodríguez-Mañas, Leocadio and Rolo, Anabela P. and Rousset, Francis and Ruskovska, Tatjana and Saraiva, Nuno and Sasson, Shlomo and Schröder, Katrin and Semen, Khrystyna and Seredenina, Tamara and Shakirzyanova, Anastasia and Smith, Geoffrey L. and Soldati, Thierry and Sousa, Bebiana C. and Spickett, Corinne M. and Stančić, Ana and Stasia, Marie José and Steinbrenner, Holger and Stepanić, Višnja and Steven, Sebastian and Tokatlidis, Kostas and Tuncay, Erkan and Turan, Belma and Ursini, Fulvio and Vacek, Jan and Vajnerova, Olga and Valentová, Kateřina and Van Breusegem, Frank and Varisli, Lokman and Veal, Elizabeth A. and Yalçın, A. Suha and Yelisyeyeva, Olha and Žarković, Neven and Zatloukalová, Martina and Zielonka, Jacek and Touyz, Rhian M. and Papapetropoulos, Andreas and Grune, Tilman and Lamas, Santiago and Schmidt, Harald H.H.W. and Di Lisa, Fabio and Daiber, Andreas",
year = "2017",
abstract = "The European Cooperation in Science and Technology (COST) provides an ideal framework to establish multi-disciplinary research networks. COST Action BM1203 (EU-ROS) represents a consortium of researchers from different disciplines who are dedicated to providing new insights and tools for better understanding redox biology and medicine and, in the long run, to finding new therapeutic strategies to target dysregulated redox processes in various diseases. This report highlights the major achievements of EU-ROS as well as research updates and new perspectives arising from its members. The EU-ROS consortium comprised more than 140 active members who worked together for four years on the topics briefly described below. The formation of reactive oxygen and nitrogen species (RONS) is an established hallmark of our aerobic environment and metabolism but RONS also act as messengers via redox regulation of essential cellular processes. The fact that many diseases have been found to be associated with oxidative stress established the theory of oxidative stress as a trigger of diseases that can be corrected by antioxidant therapy. However, while experimental studies support this thesis, clinical studies still generate controversial results, due to complex pathophysiology of oxidative stress in humans. For future improvement of antioxidant therapy and better understanding of redox-associated disease progression detailed knowledge on the sources and targets of RONS formation and discrimination of their detrimental or beneficial roles is required. In order to advance this important area of biology and medicine, highly synergistic approaches combining a variety of diverse and contrasting disciplines are needed.",
journal = "Redox Biology",
title = "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)",
volume = "13",
doi = "10.1016/j.redox.2017.05.007",
pages = "94-162"
}

Egea, J., Fabregat, I., Frapart, Y. M., Ghezzi, P., Görlach, A., Kietzmann, T., Kubaichuk, K., Knaus, U. G., Lopez, M. G., Olaso-Gonzalez, G., Petry, A., Schulz, R., Vina, J., Winyard, P., Abbas, K., Ademowo, O. S., Afonso, C. B., Andreadou, I., Antelmann, H., Antunes, F., Aslan, M., Bachschmid, M. M., Barbosa, R. M., Belousov, V., Berndt, C., Bernlohr, D., Bertrán, E., Bindoli, A., Bottari, S. P., Brito, P. M., Carrara, G., Casas, A. I., Chatzi, A., Chondrogianni, N., Conrad, M., Cooke, M. S., Costa, J. G., Cuadrado, A., My-Chan Dang, P., De Smet, B., Debelec-Butuner, B., Dias, I. H.K., Dunn, J. D., Edson, A. J., El Assar, M., El-Benna, J., Ferdinandy, P., Fernandes, A. S., Fladmark, K. E., Förstermann, U., Giniatullin, R., Giricz, Z., Görbe, A., Griffiths, H., Hampl, V., Hanf, A., Herget, J., Hernansanz-Agustín, P., Hillion, M., Huang, J., Ilikay, S., Jansen-Dürr, P., Jaquet, V., Joles, J. A., Kalyanaraman, B., Kaminskyy, D., Karbaschi, M., Kleanthous, M., Klotz, L., Korać, B., Korkmaz, K. S., Koziel, R., Kračun, D., Krause, K., Křen, V., Krieg, T., Laranjinha, J., Lazou, A., Li, H., Martínez-Ruiz, A., Matsui, R., McBean, G. J., Meredith, S. P., Messens, J., Miguel, V., Mikhed, Y., Milisav, I., Milković, L., Miranda-Vizuete, A., Mojović, M., Monsalve, M., Mouthuy, P., Mulvey, J., Münzel, T., Muzykantov, V., Nguyen, I. T.N., Oelze, M., Oliveira, N. G., Palmeira, C. M., Papaevgeniou, N., Pavićević, A., Pedre, B., Peyrot, F., Phylactides, M., Pircalabioru, G. G., Pitt, A. R., Poulsen, H. E., Prieto, I., Rigobello, M. P., Robledinos-Antón, N., Rodríguez-Mañas, L., Rolo, A. P., Rousset, F., Ruskovska, T., Saraiva, N., Sasson, S., Schröder, K., Semen, K., Seredenina, T., Shakirzyanova, A., Smith, G. L., Soldati, T., Sousa, B. C., Spickett, C. M., Stančić, A., Stasia, M. J., Steinbrenner, H., Stepanić, V., Steven, S., Tokatlidis, K., Tuncay, E., Turan, B., Ursini, F., Vacek, J., Vajnerova, O., Valentová, K., Van Breusegem, F., Varisli, L., Veal, E. A., Yalçın, A. S., Yelisyeyeva, O., Žarković, N., Zatloukalová, M., Zielonka, J., Touyz, R. M., Papapetropoulos, A., Grune, T., Lamas, S., Schmidt, H. H.H.W., Di Lisa, F.,& Daiber, A.. (2017). European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). in Redox Biology, 13, 94-162.
https://doi.org/10.1016/j.redox.2017.05.007

Egea J, Fabregat I, Frapart YM, Ghezzi P, Görlach A, Kietzmann T, Kubaichuk K, Knaus UG, Lopez MG, Olaso-Gonzalez G, Petry A, Schulz R, Vina J, Winyard P, Abbas K, Ademowo OS, Afonso CB, Andreadou I, Antelmann H, Antunes F, Aslan M, Bachschmid MM, Barbosa RM, Belousov V, Berndt C, Bernlohr D, Bertrán E, Bindoli A, Bottari SP, Brito PM, Carrara G, Casas AI, Chatzi A, Chondrogianni N, Conrad M, Cooke MS, Costa JG, Cuadrado A, My-Chan Dang P, De Smet B, Debelec-Butuner B, Dias IH, Dunn JD, Edson AJ, El Assar M, El-Benna J, Ferdinandy P, Fernandes AS, Fladmark KE, Förstermann U, Giniatullin R, Giricz Z, Görbe A, Griffiths H, Hampl V, Hanf A, Herget J, Hernansanz-Agustín P, Hillion M, Huang J, Ilikay S, Jansen-Dürr P, Jaquet V, Joles JA, Kalyanaraman B, Kaminskyy D, Karbaschi M, Kleanthous M, Klotz L, Korać B, Korkmaz KS, Koziel R, Kračun D, Krause K, Křen V, Krieg T, Laranjinha J, Lazou A, Li H, Martínez-Ruiz A, Matsui R, McBean GJ, Meredith SP, Messens J, Miguel V, Mikhed Y, Milisav I, Milković L, Miranda-Vizuete A, Mojović M, Monsalve M, Mouthuy P, Mulvey J, Münzel T, Muzykantov V, Nguyen IT, Oelze M, Oliveira NG, Palmeira CM, Papaevgeniou N, Pavićević A, Pedre B, Peyrot F, Phylactides M, Pircalabioru GG, Pitt AR, Poulsen HE, Prieto I, Rigobello MP, Robledinos-Antón N, Rodríguez-Mañas L, Rolo AP, Rousset F, Ruskovska T, Saraiva N, Sasson S, Schröder K, Semen K, Seredenina T, Shakirzyanova A, Smith GL, Soldati T, Sousa BC, Spickett CM, Stančić A, Stasia MJ, Steinbrenner H, Stepanić V, Steven S, Tokatlidis K, Tuncay E, Turan B, Ursini F, Vacek J, Vajnerova O, Valentová K, Van Breusegem F, Varisli L, Veal EA, Yalçın AS, Yelisyeyeva O, Žarković N, Zatloukalová M, Zielonka J, Touyz RM, Papapetropoulos A, Grune T, Lamas S, Schmidt HH, Di Lisa F, Daiber A. European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). in Redox Biology. 2017;13:94-162.
doi:10.1016/j.redox.2017.05.007 .

Egea, Javier, Fabregat, Isabel, Frapart, Yves M., Ghezzi, Pietro, Görlach, Agnes, Kietzmann, Thomas, Kubaichuk, Kateryna, Knaus, Ulla G., Lopez, Manuela G., Olaso-Gonzalez, Gloria, Petry, Andreas, Schulz, Rainer, Vina, Jose, Winyard, Paul, Abbas, Kahina, Ademowo, Opeyemi S., Afonso, Catarina B., Andreadou, Ioanna, Antelmann, Haike, Antunes, Fernando, Aslan, Mutay, Bachschmid, Markus M., Barbosa, Rui M., Belousov, Vsevolod, Berndt, Carsten, Bernlohr, David, Bertrán, Esther, Bindoli, Alberto, Bottari, Serge P., Brito, Paula M., Carrara, Guia, Casas, Ana I., Chatzi, Afroditi, Chondrogianni, Niki, Conrad, Marcus, Cooke, Marcus S., Costa, João G., Cuadrado, Antonio, My-Chan Dang, Pham, De Smet, Barbara, Debelec-Butuner, Bilge, Dias, Irundika H.K., Dunn, Joe Dan, Edson, Amanda J., El Assar, Mariam, El-Benna, Jamel, Ferdinandy, Péter, Fernandes, Ana S., Fladmark, Kari E., Förstermann, Ulrich, Giniatullin, Rashid, Giricz, Zoltán, Görbe, Anikó, Griffiths, Helen, Hampl, Vaclav, Hanf, Alina, Herget, Jan, Hernansanz-Agustín, Pablo, Hillion, Melanie, Huang, Jingjing, Ilikay, Serap, Jansen-Dürr, Pidder, Jaquet, Vincent, Joles, Jaap A., Kalyanaraman, Balaraman, Kaminskyy, Danylo, Karbaschi, Mahsa, Kleanthous, Marina, Klotz, Lars-Oliver, Korać, Bato, Korkmaz, Kemal Sami, Koziel, Rafal, Kračun, Damir, Krause, Karl-Heinz, Křen, Vladimír, Krieg, Thomas, Laranjinha, João, Lazou, Antigone, Li, Huige, Martínez-Ruiz, Antonio, Matsui, Reiko, McBean, Gethin J., Meredith, Stuart P., Messens, Joris, Miguel, Verónica, Mikhed, Yuliya, Milisav, Irina, Milković, Lidija, Miranda-Vizuete, Antonio, Mojović, Miloš, Monsalve, María, Mouthuy, Pierre-Alexis, Mulvey, John, Münzel, Thomas, Muzykantov, Vladimir, Nguyen, Isabel T.N., Oelze, Matthias, Oliveira, Nuno G., Palmeira, Carlos M., Papaevgeniou, Nikoletta, Pavićević, Aleksandra, Pedre, Brandán, Peyrot, Fabienne, Phylactides, Marios, Pircalabioru, Gratiela G., Pitt, Andrew R., Poulsen, Henrik E., Prieto, Ignacio, Rigobello, Maria Pia, Robledinos-Antón, Natalia, Rodríguez-Mañas, Leocadio, Rolo, Anabela P., Rousset, Francis, Ruskovska, Tatjana, Saraiva, Nuno, Sasson, Shlomo, Schröder, Katrin, Semen, Khrystyna, Seredenina, Tamara, Shakirzyanova, Anastasia, Smith, Geoffrey L., Soldati, Thierry, Sousa, Bebiana C., Spickett, Corinne M., Stančić, Ana, Stasia, Marie José, Steinbrenner, Holger, Stepanić, Višnja, Steven, Sebastian, Tokatlidis, Kostas, Tuncay, Erkan, Turan, Belma, Ursini, Fulvio, Vacek, Jan, Vajnerova, Olga, Valentová, Kateřina, Van Breusegem, Frank, Varisli, Lokman, Veal, Elizabeth A., Yalçın, A. Suha, Yelisyeyeva, Olha, Žarković, Neven, Zatloukalová, Martina, Zielonka, Jacek, Touyz, Rhian M., Papapetropoulos, Andreas, Grune, Tilman, Lamas, Santiago, Schmidt, Harald H.H.W., Di Lisa, Fabio, Daiber, Andreas, "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" in Redox Biology, 13 (2017):94-162,
https://doi.org/10.1016/j.redox.2017.05.007 . .

TY  - CONF
AU  - Novaković, Lazar
AU  - Živković, Suzana
AU  - Popović-Bijelić, Ana
AU  - Mišić, Danijela
AU  - Mojović, Miloš
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6189
AB  - Electron paramagnetic resonance spectroscopy (EPR) was applied for in vivo detection of oxidative stress induced by high ionic strengths in Centaurium erythraea Rafn (Gentianaceae), a herbaceous perennial often found on saline soils. Shoots of C. erythraea were cultured in vitro for 4 weeks on solid 1/2 MS medium, or 1/2 MS medium supplemented with 200 mM NaCl to induce salt stress and thus stimulate the production of reactive oxygen species (ROS), which is a common response of plant cells subjected to various types of biotic or abiotic stresses. The reduction of a stable cell-permeable aminoxyl radical spin probe, Tempo, was measured to assess the oxidative status of the control and salt-treated samples. In both types of samples, the reduction of Tempo showed zero-order kinetics. After one hour, the control reduced only 9% of the initial amount of the spin probe, whereas the sample grown with 200 mM NaCl reduced it by 20%. This may indicate that the plants grown in the presence of 200 mM NaCl produced higher amounts of ROS which are able to reduce the aminoxyl radical. The obtained results indicate that EPR can be used as a method for in vivo evaluation of the redox state of plants under stress conditions, and could also be useful in determining the salt-stress tolerance of plants. Further
studies, including different salt concentrations, and plant species, have to be conducted to verify these findings.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn
SP  - 178
EP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6189
ER  - 

@conference{
author = "Novaković, Lazar and Živković, Suzana and Popović-Bijelić, Ana and Mišić, Danijela and Mojović, Miloš",
year = "2015",
abstract = "Electron paramagnetic resonance spectroscopy (EPR) was applied for in vivo detection of oxidative stress induced by high ionic strengths in Centaurium erythraea Rafn (Gentianaceae), a herbaceous perennial often found on saline soils. Shoots of C. erythraea were cultured in vitro for 4 weeks on solid 1/2 MS medium, or 1/2 MS medium supplemented with 200 mM NaCl to induce salt stress and thus stimulate the production of reactive oxygen species (ROS), which is a common response of plant cells subjected to various types of biotic or abiotic stresses. The reduction of a stable cell-permeable aminoxyl radical spin probe, Tempo, was measured to assess the oxidative status of the control and salt-treated samples. In both types of samples, the reduction of Tempo showed zero-order kinetics. After one hour, the control reduced only 9% of the initial amount of the spin probe, whereas the sample grown with 200 mM NaCl reduced it by 20%. This may indicate that the plants grown in the presence of 200 mM NaCl produced higher amounts of ROS which are able to reduce the aminoxyl radical. The obtained results indicate that EPR can be used as a method for in vivo evaluation of the redox state of plants under stress conditions, and could also be useful in determining the salt-stress tolerance of plants. Further
studies, including different salt concentrations, and plant species, have to be conducted to verify these findings.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn",
pages = "178-179",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6189"
}

Novaković, L., Živković, S., Popović-Bijelić, A., Mišić, D.,& Mojović, M.. (2015). Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 178-179.
https://hdl.handle.net/21.15107/rcub_ibiss_6189

Novaković L, Živković S, Popović-Bijelić A, Mišić D, Mojović M. Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:178-179.
https://hdl.handle.net/21.15107/rcub_ibiss_6189 .

Novaković, Lazar, Živković, Suzana, Popović-Bijelić, Ana, Mišić, Danijela, Mojović, Miloš, "Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):178-179,
https://hdl.handle.net/21.15107/rcub_ibiss_6189 .

TY  - JOUR
AU  - Grubor-Lajšić, Gordana
AU  - Petri, Edward
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko
AU  - Worland, Michael Roger
AU  - Clark, Melody S.
AU  - Mojović, Miloš
AU  - Blagojević, Duško
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6338
AB  - The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.
PB  - Hoboken: John Wiley and Sons
T2  - Archives of Insect Biochemistry and Physiology
T1  - Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)
IS  - 2
VL  - 82
DO  - 10.1002/arch.21073
SP  - 59
EP  - 70
ER  - 

@article{
author = "Grubor-Lajšić, Gordana and Petri, Edward and Kojić, Danijela and Purać, Jelena and Popović, Željko and Worland, Michael Roger and Clark, Melody S. and Mojović, Miloš and Blagojević, Duško",
year = "2013",
abstract = "The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Archives of Insect Biochemistry and Physiology",
title = "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)",
number = "2",
volume = "82",
doi = "10.1002/arch.21073",
pages = "59-70"
}

Grubor-Lajšić, G., Petri, E., Kojić, D., Purać, J., Popović, Ž., Worland, M. R., Clark, M. S., Mojović, M.,& Blagojević, D.. (2013). Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology
Hoboken: John Wiley and Sons., 82(2), 59-70.
https://doi.org/10.1002/arch.21073

Grubor-Lajšić G, Petri E, Kojić D, Purać J, Popović Ž, Worland MR, Clark MS, Mojović M, Blagojević D. Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology. 2013;82(2):59-70.
doi:10.1002/arch.21073 .

Grubor-Lajšić, Gordana, Petri, Edward, Kojić, Danijela, Purać, Jelena, Popović, Željko, Worland, Michael Roger, Clark, Melody S., Mojović, Miloš, Blagojević, Duško, "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)" in Archives of Insect Biochemistry and Physiology, 82, no. 2 (2013):59-70,
https://doi.org/10.1002/arch.21073 . .