TY  - CHAP
AU  - Tovilović-Kovačević, Gordana
AU  - Zogović, Nevena
AU  - Krstić Milošević, Dijana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/123456789/3896
AB  - Gentianaceae have a long history of use as traditional remedies for treatment of various ailments. The medicinal properties of crude herbal drug are attributed to bitter glycosides, flavonoids, and xanthones, the main plant secondary metabolites. These plant-derived molecules, especially naturally occurring xanthones, possess a broad spectrum of bioactivity like anticarcinogenic, antimicrobial, neuroprotective, antidiabetic, cardio-protective. The most of Gentianaceae species are rare and endangered by uncontrolled overharvesting and influences of various environmental factors (habitat loss, climate change, and invasive species spreading). Decline of Gentianaceae species poses a high risk to the loss of enormous diversity of potentially bioactive compounds. In this chapter we will summarize pharmacological activities of identified secondary metabolites from endangered species belonging to four Gentianaceae genera (Gentiana, Gentianella, Centaurium, Swertia), as well as importance of biodiversity conservation in context of their biotherapeutic potential.
PB  - Academic Press
T2  - Biodiversity and Biomedicine Our Future
T1  - Secondary metabolites from endangered Gentiana, Gentianella, Centaurium, and Swertia species (Gentianaceae): promising natural biotherapeutics
DO  - 10.1016/B978-0-12-819541-3.00019-0
SP  - 335
EP  - 384
ER  - 

@inbook{
author = "Tovilović-Kovačević, Gordana and Zogović, Nevena and Krstić Milošević, Dijana",
year = "2020",
abstract = "Gentianaceae have a long history of use as traditional remedies for treatment of various ailments. The medicinal properties of crude herbal drug are attributed to bitter glycosides, flavonoids, and xanthones, the main plant secondary metabolites. These plant-derived molecules, especially naturally occurring xanthones, possess a broad spectrum of bioactivity like anticarcinogenic, antimicrobial, neuroprotective, antidiabetic, cardio-protective. The most of Gentianaceae species are rare and endangered by uncontrolled overharvesting and influences of various environmental factors (habitat loss, climate change, and invasive species spreading). Decline of Gentianaceae species poses a high risk to the loss of enormous diversity of potentially bioactive compounds. In this chapter we will summarize pharmacological activities of identified secondary metabolites from endangered species belonging to four Gentianaceae genera (Gentiana, Gentianella, Centaurium, Swertia), as well as importance of biodiversity conservation in context of their biotherapeutic potential.",
publisher = "Academic Press",
journal = "Biodiversity and Biomedicine Our Future",
booktitle = "Secondary metabolites from endangered Gentiana, Gentianella, Centaurium, and Swertia species (Gentianaceae): promising natural biotherapeutics",
doi = "10.1016/B978-0-12-819541-3.00019-0",
pages = "335-384"
}

Tovilović-Kovačević, G., Zogović, N.,& Krstić Milošević, D.. (2020). Secondary metabolites from endangered Gentiana, Gentianella, Centaurium, and Swertia species (Gentianaceae): promising natural biotherapeutics. in Biodiversity and Biomedicine Our Future
Academic Press., 335-384.
https://doi.org/10.1016/B978-0-12-819541-3.00019-0

Tovilović-Kovačević G, Zogović N, Krstić Milošević D. Secondary metabolites from endangered Gentiana, Gentianella, Centaurium, and Swertia species (Gentianaceae): promising natural biotherapeutics. in Biodiversity and Biomedicine Our Future. 2020;:335-384.
doi:10.1016/B978-0-12-819541-3.00019-0 .

Tovilović-Kovačević, Gordana, Zogović, Nevena, Krstić Milošević, Dijana, "Secondary metabolites from endangered Gentiana, Gentianella, Centaurium, and Swertia species (Gentianaceae): promising natural biotherapeutics" in Biodiversity and Biomedicine Our Future (2020):335-384,
https://doi.org/10.1016/B978-0-12-819541-3.00019-0 . .

TY  - JOUR
AU  - Vidović, Nevena
AU  - Ranković, Slavica
AU  - Oreščanin Dušić, Zorana
AU  - Debeljak-Martačić, Jasmina
AU  - Popović, Tamara
AU  - Tomić, Mirko
AU  - Glibetić, Marija
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0042-84501800122V
UR  - https://radar.ibiss.bg.ac.rs/123456789/3878
AB  - Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants
PB  - National Library of Serbia
T2  - Vojnosanitetski pregled
T1  - The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study
IS  - 6
VL  - 77
DO  - 10.2298/VSP180326122V
SP  - 641
EP  - 646
ER  - 

@article{
author = "Vidović, Nevena and Ranković, Slavica and Oreščanin Dušić, Zorana and Debeljak-Martačić, Jasmina and Popović, Tamara and Tomić, Mirko and Glibetić, Marija",
year = "2020",
abstract = "Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants",
publisher = "National Library of Serbia",
journal = "Vojnosanitetski pregled",
title = "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study",
number = "6",
volume = "77",
doi = "10.2298/VSP180326122V",
pages = "641-646"
}

Vidović, N., Ranković, S., Oreščanin Dušić, Z., Debeljak-Martačić, J., Popović, T., Tomić, M.,& Glibetić, M.. (2020). The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled
National Library of Serbia., 77(6), 641-646.
https://doi.org/10.2298/VSP180326122V

Vidović N, Ranković S, Oreščanin Dušić Z, Debeljak-Martačić J, Popović T, Tomić M, Glibetić M. The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled. 2020;77(6):641-646.
doi:10.2298/VSP180326122V .

Vidović, Nevena, Ranković, Slavica, Oreščanin Dušić, Zorana, Debeljak-Martačić, Jasmina, Popović, Tamara, Tomić, Mirko, Glibetić, Marija, "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study" in Vojnosanitetski pregled, 77, no. 6 (2020):641-646,
https://doi.org/10.2298/VSP180326122V . .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Soković-Bajić, Svetlana
AU  - Bojović, Katarina
AU  - Vojnović-Milutinović, Danijela
AU  - Tomić, Mirko
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5431
AB  - Objective: Clinical reports indicated increased incidence of gastrointestinal (GI) disturbances and dysbiosis in children with neurodevelopmental disorders (NDD) as well as acceleration of the recovery after the dysbiosis treatment. The aim of this study was to evaluate gut microbiota diversity and to identify bacterial strains which incidences were different between patients’ and control group. This is the first study evaluating gut microbiota diversity and composition in children with NDD.
Conclusions: Our study reveals that the intestinal microbiota from children with NDDs differs from the microbiota of healthy children. Similarity of microbiota composition in patient groups with similar neurological and behavioral symptoms indicate that intestinal microbiota might have role in pathophysiology of NDD. Supplementation with several health promoting strains, could be safe adjuvant therapy in treatment of NDD accompanied with GI disturbances.
PB  - Wolters Kluwer Health
C3  - 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
T1  - Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders
VL  - 54
DO  - 10.1097/MCG.0000000000001292
ER  - 

@conference{
author = "Ignjatović, Đurđica and Soković-Bajić, Svetlana and Bojović, Katarina and Vojnović-Milutinović, Danijela and Tomić, Mirko and Golić, Nataša and Tolinački, Maja",
year = "2019",
abstract = "Objective: Clinical reports indicated increased incidence of gastrointestinal (GI) disturbances and dysbiosis in children with neurodevelopmental disorders (NDD) as well as acceleration of the recovery after the dysbiosis treatment. The aim of this study was to evaluate gut microbiota diversity and to identify bacterial strains which incidences were different between patients’ and control group. This is the first study evaluating gut microbiota diversity and composition in children with NDD.
Conclusions: Our study reveals that the intestinal microbiota from children with NDDs differs from the microbiota of healthy children. Similarity of microbiota composition in patient groups with similar neurological and behavioral symptoms indicate that intestinal microbiota might have role in pathophysiology of NDD. Supplementation with several health promoting strains, could be safe adjuvant therapy in treatment of NDD accompanied with GI disturbances.",
publisher = "Wolters Kluwer Health",
journal = "10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy",
title = "Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders",
volume = "54",
doi = "10.1097/MCG.0000000000001292"
}

Ignjatović, Đ., Soković-Bajić, S., Bojović, K., Vojnović-Milutinović, D., Tomić, M., Golić, N.,& Tolinački, M.. (2019). Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
Wolters Kluwer Health., 54.
https://doi.org/10.1097/MCG.0000000000001292

Ignjatović Đ, Soković-Bajić S, Bojović K, Vojnović-Milutinović D, Tomić M, Golić N, Tolinački M. Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. 2019;54.
doi:10.1097/MCG.0000000000001292 .

Ignjatović, Đurđica, Soković-Bajić, Svetlana, Bojović, Katarina, Vojnović-Milutinović, Danijela, Tomić, Mirko, Golić, Nataša, Tolinački, Maja, "Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders" in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy, 54 (2019),
https://doi.org/10.1097/MCG.0000000000001292 . .

TY  - JOUR
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović, Snježana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Maria
PY  - 2017
UR  - http://lipidworld.biomedcentral.com/articles/10.1186/s12944-017-0483-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2768
AB  - Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.
T2  - Lipids in Health and Disease
T1  - Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes
IS  - 1
VL  - 16
DO  - 10.1186/s12944-017-0483-9
SP  - 94
EP  - 94
ER  - 

@article{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović, Snježana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Maria",
year = "2017",
abstract = "Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.",
journal = "Lipids in Health and Disease",
title = "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes",
number = "1",
volume = "16",
doi = "10.1186/s12944-017-0483-9",
pages = "94-94"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović, S., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2017). Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease, 16(1), 94-94.
https://doi.org/10.1186/s12944-017-0483-9

Ranković S, Popović T, Debeljak Martačić J, Petrović S, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease. 2017;16(1):94-94.
doi:10.1186/s12944-017-0483-9 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović, Snježana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Maria, "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes" in Lipids in Health and Disease, 16, no. 1 (2017):94-94,
https://doi.org/10.1186/s12944-017-0483-9 . .

TY  - JOUR
AU  - Zec, Manja
AU  - Debeljak Martačić, Jasmina
AU  - Ranković, Slavica
AU  - Pokimica, Biljana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Glibetić, Maria
AU  - Popović, Tamara
PY  - 2017
UR  - http://www.degruyter.com/view/j/acve.2017.67.issue-1/acve-2017-0010/acve-2017-0010.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2731
AB  - A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.
T2  - Acta Veterinaria
T1  - Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats
IS  - 1
VL  - 67
DO  - 10.1515/acve-2017-0010
SP  - 107
EP  - 120
ER  - 

@article{
author = "Zec, Manja and Debeljak Martačić, Jasmina and Ranković, Slavica and Pokimica, Biljana and Tomić, Mirko and Ignjatović, Đurđica and Glibetić, Maria and Popović, Tamara",
year = "2017",
abstract = "A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.",
journal = "Acta Veterinaria",
title = "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats",
number = "1",
volume = "67",
doi = "10.1515/acve-2017-0010",
pages = "107-120"
}

Zec, M., Debeljak Martačić, J., Ranković, S., Pokimica, B., Tomić, M., Ignjatović, Đ., Glibetić, M.,& Popović, T.. (2017). Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria, 67(1), 107-120.
https://doi.org/10.1515/acve-2017-0010

Zec M, Debeljak Martačić J, Ranković S, Pokimica B, Tomić M, Ignjatović Đ, Glibetić M, Popović T. Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria. 2017;67(1):107-120.
doi:10.1515/acve-2017-0010 .

Zec, Manja, Debeljak Martačić, Jasmina, Ranković, Slavica, Pokimica, Biljana, Tomić, Mirko, Ignjatović, Đurđica, Glibetić, Maria, Popović, Tamara, "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats" in Acta Veterinaria, 67, no. 1 (2017):107-120,
https://doi.org/10.1515/acve-2017-0010 . .

TY  - JOUR
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Krstić Milošević, Dijana
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Glibetić, Marija
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/123456789/3894
AB  - The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of
Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic
compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable
health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate
the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice
reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic
compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three
various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 +
20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution
was significantly elevated from the second or third week onward, which was most expressed in the ARO
group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field
tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also
expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate
the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml
per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The
in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the
possible in vivo mechanisms for such behavioral actions.
PB  - Royal Society of Chemistry
T2  - Food & Function
T1  - Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice
VL  - 7
DO  - 10.1039/c6fo00321d
SP  - 3111
EP  - 3120
ER  - 

@article{
author = "Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Krstić Milošević, Dijana and Ranković, Slavica and Popović, Tamara and Glibetić, Marija",
year = "2016",
abstract = "The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of
Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic
compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable
health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate
the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice
reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic
compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three
various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 +
20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution
was significantly elevated from the second or third week onward, which was most expressed in the ARO
group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field
tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also
expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate
the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml
per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The
in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the
possible in vivo mechanisms for such behavioral actions.",
publisher = "Royal Society of Chemistry",
journal = "Food & Function",
title = "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice",
volume = "7",
doi = "10.1039/c6fo00321d",
pages = "3111-3120"
}

Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Krstić Milošević, D., Ranković, S., Popović, T.,& Glibetić, M.. (2016). Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function
Royal Society of Chemistry., 7, 3111-3120.
https://doi.org/10.1039/c6fo00321d

Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Krstić Milošević D, Ranković S, Popović T, Glibetić M. Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function. 2016;7:3111-3120.
doi:10.1039/c6fo00321d .

Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Krstić Milošević, Dijana, Ranković, Slavica, Popović, Tamara, Glibetić, Marija, "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice" in Food & Function, 7 (2016):3111-3120,
https://doi.org/10.1039/c6fo00321d . .

TY  - JOUR
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Krstić Milošević, Dijana
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Glibetić, Marija
PY  - 2016
UR  - https://pubs.rsc.org/en/content/articlelanding/2016/FO/C6FO00321D#!divAbstract
UR  - https://radar.ibiss.bg.ac.rs/123456789/3893
AB  - The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 + 20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution was significantly elevated from the second or third week onward, which was most expressed in the ARO group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the possible in vivo mechanisms for such behavioral actions.
PB  - Royal Society of Chemistry
T2  - Food & Function
T1  - Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice
VL  - 7
DO  - 10.1039/c6fo00321d
SP  - 3111
EP  - 3120
ER  - 

@article{
author = "Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Krstić Milošević, Dijana and Ranković, Slavica and Popović, Tamara and Glibetić, Marija",
year = "2016",
abstract = "The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 + 20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution was significantly elevated from the second or third week onward, which was most expressed in the ARO group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the possible in vivo mechanisms for such behavioral actions.",
publisher = "Royal Society of Chemistry",
journal = "Food & Function",
title = "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice",
volume = "7",
doi = "10.1039/c6fo00321d",
pages = "3111-3120"
}

Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Krstić Milošević, D., Ranković, S., Popović, T.,& Glibetić, M.. (2016). Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function
Royal Society of Chemistry., 7, 3111-3120.
https://doi.org/10.1039/c6fo00321d

Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Krstić Milošević D, Ranković S, Popović T, Glibetić M. Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice. in Food & Function. 2016;7:3111-3120.
doi:10.1039/c6fo00321d .

Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Krstić Milošević, Dijana, Ranković, Slavica, Popović, Tamara, Glibetić, Marija, "Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice" in Food & Function, 7 (2016):3111-3120,
https://doi.org/10.1039/c6fo00321d . .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović Oggiano, Gordana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Miler, Marko
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6347
AB  - Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
T1  - Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats
SP  - 139
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6347
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović Oggiano, Gordana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Miler, Marko and Glibetić, Marija",
year = "2015",
abstract = "Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia",
title = "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6347"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović Oggiano, G., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Miler, M.,& Glibetić, M.. (2015). Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347

Ranković S, Popović T, Debeljak Martačić J, Petrović Oggiano G, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Miler M, Glibetić M. Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia. 2015;:139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović Oggiano, Gordana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Miler, Marko, Glibetić, Marija, "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats" in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia (2015):139-139,
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Slavica
AU  - Glibetić, Marija
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6349
AB  - Aronia melanocarpa fruits are among the richest plant sources of phenolic substances, mainly anthocyanins. Aronia fruit juice and derived flavonoids were evaluated in a number of studies for high antioxidative activity, whose sequels were many beneficial physiological and metabolic effects, like hepato-protective, anti-inflammatory and antitumor. This study was designed to explore the effects of one month intensive consumption of aronia juice on phospholipid compositions in rat liver. Three experimental groups of young male rats were being supplied for 34 days with different drinking solutions ad libitum: 1. (ARO group) 20% (m/m) master aronia juice in tap water; 2. (MIX) 5% master aronia juice + 15% juice reconstruct (without flavonoids); 3. (PLC) 20% juice reconstruct. The animals were sacrificed on day 35 and their livers were weighed and used for analysis. The average mass of liver in ARO group was somewhat higher (13-14%) than in other two groups. In comparison with PLC, both experimental groups exhibited significantly lower proportions of stearic acid (18:0) and arachidonic acid (20:4), whilst the level of MUFA was increased only in MIX group on account of vaccenic acid elevation (18:1(7)). Also, there was a certain increase of omega-3 PUFA with a decline of omega 6/omega-3 PUFA ratio in both groups that consumed aronia juice. All these changes in the liver phospholipid compositions, which were induced by everyday drinking of flavonoid-rich aronia juice, appeared to be favorable regarding the healthy ratios of the liver fatty acid contents.
PB  - elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver
SP  - 86
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6349
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Slavica and Glibetić, Marija and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Tomić, Mirko",
year = "2015",
abstract = "Aronia melanocarpa fruits are among the richest plant sources of phenolic substances, mainly anthocyanins. Aronia fruit juice and derived flavonoids were evaluated in a number of studies for high antioxidative activity, whose sequels were many beneficial physiological and metabolic effects, like hepato-protective, anti-inflammatory and antitumor. This study was designed to explore the effects of one month intensive consumption of aronia juice on phospholipid compositions in rat liver. Three experimental groups of young male rats were being supplied for 34 days with different drinking solutions ad libitum: 1. (ARO group) 20% (m/m) master aronia juice in tap water; 2. (MIX) 5% master aronia juice + 15% juice reconstruct (without flavonoids); 3. (PLC) 20% juice reconstruct. The animals were sacrificed on day 35 and their livers were weighed and used for analysis. The average mass of liver in ARO group was somewhat higher (13-14%) than in other two groups. In comparison with PLC, both experimental groups exhibited significantly lower proportions of stearic acid (18:0) and arachidonic acid (20:4), whilst the level of MUFA was increased only in MIX group on account of vaccenic acid elevation (18:1(7)). Also, there was a certain increase of omega-3 PUFA with a decline of omega 6/omega-3 PUFA ratio in both groups that consumed aronia juice. All these changes in the liver phospholipid compositions, which were induced by everyday drinking of flavonoid-rich aronia juice, appeared to be favorable regarding the healthy ratios of the liver fatty acid contents.",
publisher = "elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver",
pages = "86-87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6349"
}

Ranković, S., Popović, T., Debeljak Martačić, S., Glibetić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Tomić, M.. (2015). Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 86-87.
https://hdl.handle.net/21.15107/rcub_ibiss_6349

Ranković S, Popović T, Debeljak Martačić S, Glibetić M, Ignjatović Đ, Tovilović-Kovačević G, Tomić M. Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:86-87.
https://hdl.handle.net/21.15107/rcub_ibiss_6349 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Slavica, Glibetić, Marija, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Tomić, Mirko, "Drinking of flavonoid-rich juice of Aronia melanocarpa may affect fatty acid phospholipid compositions in rat liver" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):86-87,
https://hdl.handle.net/21.15107/rcub_ibiss_6349 .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Slavica
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6344
AB  - The fruits of Aronia melanocarpa are rich in anthocyanins, plant pigments with a number of registered beneficial effects on human health (e.g. antioxidative, anti-inflammatory, immunomodulatory). Some limited studies have also shown their effects on behavior and cognitive functions in experimental animals. This study was designed to explore the effects of unlimited consumption of diluted aronia juice on rat behavior. Young male Wistar rats were divided into control group (CTL; N=12) with tap water provided ad libitum, and 3 experimental groups (N=8) supplied with drinking solutions of 20% mixtures (m/m) in tap water: (ARO) 100% master aronia juice; (MIX) 25% master aronia juice + 75% placebo solution (juice reconstruct without flavonoids); (PLC) 100% placebo. Rats were being allowed to drink these solutions without limits for 34 days, where on days 31-34 three behavioral tests were performed. These tests revealed that only ARO group exhibited significant behavioral changes: augmented locomotion in an open field test, anxiolytic-like action in elevated plus maze, as well as an increase in active swimming in forced swimming test (which is relevant to antidepressant-like action). These results suggest that everyday drinking of diluted aronia juice (between 6 and 12 g of master aronia juice daily per rat) with high flavonoid content (about 0.12%) for about a month, may induce several stimulant behavioral actions in experimental animals.
PB  - Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats
SP  - 78
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6344
ER  - 

@conference{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Tomić, Mirko and Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Slavica and Glibetić, Marija",
year = "2015",
abstract = "The fruits of Aronia melanocarpa are rich in anthocyanins, plant pigments with a number of registered beneficial effects on human health (e.g. antioxidative, anti-inflammatory, immunomodulatory). Some limited studies have also shown their effects on behavior and cognitive functions in experimental animals. This study was designed to explore the effects of unlimited consumption of diluted aronia juice on rat behavior. Young male Wistar rats were divided into control group (CTL; N=12) with tap water provided ad libitum, and 3 experimental groups (N=8) supplied with drinking solutions of 20% mixtures (m/m) in tap water: (ARO) 100% master aronia juice; (MIX) 25% master aronia juice + 75% placebo solution (juice reconstruct without flavonoids); (PLC) 100% placebo. Rats were being allowed to drink these solutions without limits for 34 days, where on days 31-34 three behavioral tests were performed. These tests revealed that only ARO group exhibited significant behavioral changes: augmented locomotion in an open field test, anxiolytic-like action in elevated plus maze, as well as an increase in active swimming in forced swimming test (which is relevant to antidepressant-like action). These results suggest that everyday drinking of diluted aronia juice (between 6 and 12 g of master aronia juice daily per rat) with high flavonoid content (about 0.12%) for about a month, may induce several stimulant behavioral actions in experimental animals.",
publisher = "Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats",
pages = "78-79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6344"
}

Ignjatović, Đ., Tovilović-Kovačević, G., Tomić, M., Ranković, S., Popović, T., Debeljak Martačić, S.,& Glibetić, M.. (2015). The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"., 78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6344

Ignjatović Đ, Tovilović-Kovačević G, Tomić M, Ranković S, Popović T, Debeljak Martačić S, Glibetić M. The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6344 .

Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Tomić, Mirko, Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Slavica, Glibetić, Marija, "The stimulant behavioral effects of anthocyanin-rich juice of Aronia melanocarpa in rats" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):78-79,
https://hdl.handle.net/21.15107/rcub_ibiss_6344 .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Slavica
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6340
AB  - The aim of this study was to assess how the unlimited drinking of Aronia melanocarpa fruit juice, rich in phenolic substances (1.177 g L-¹ gallic acid equivalents), may affect the levels of several biochemical markers in the rat plasma: glucose (Glu), total cholesterol (ChT) and its fractions (HDL and LDL), triglycerides (TG) and transaminases (ALT and AST). Young male rats were being supplied with 3 combinations of juice solutions in drinking water for 34 days, and their blood samples were collected for analysis after animal sacrifices on day 35. The experimental groups contained 20% juice solutions in tap water of: (ARO) 100% master aronia juice, (MIX) 25% master aronia juice + 75% juice reconstruct (without flavonoids), and (PLC) 100% juice reconstruct, and they were compared with a (CTL) control group on pure tap water. Biochemical analyses of plasma on Cobas c-111 analyzer showed a significant 20% decrease of Glu in ARO group in comparison to CTL. ChT was significantly higher in MIX and PLC in relation to both CTL and ARO groups, which arose mainly from LDL elevations. Also, there was a certain (30%), but insignificant increase of TG in MIX and PLC vs. CTL. The effects of aronia on transaminases were registered only for ALT, whose level in ARO was about 3-fold of that in CTL group. In conclusion, everyday free drinking of flavonoid-rich aronia juice solutions appeared to have global hypoglycemic effect in normal rats and, also, certain hypolipidemic effects, when hyperlipidemia was induced probably with higher sugar consumption.
PB  - Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6340
ER  - 

@conference{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Tomić, Mirko and Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Slavica and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to assess how the unlimited drinking of Aronia melanocarpa fruit juice, rich in phenolic substances (1.177 g L-¹ gallic acid equivalents), may affect the levels of several biochemical markers in the rat plasma: glucose (Glu), total cholesterol (ChT) and its fractions (HDL and LDL), triglycerides (TG) and transaminases (ALT and AST). Young male rats were being supplied with 3 combinations of juice solutions in drinking water for 34 days, and their blood samples were collected for analysis after animal sacrifices on day 35. The experimental groups contained 20% juice solutions in tap water of: (ARO) 100% master aronia juice, (MIX) 25% master aronia juice + 75% juice reconstruct (without flavonoids), and (PLC) 100% juice reconstruct, and they were compared with a (CTL) control group on pure tap water. Biochemical analyses of plasma on Cobas c-111 analyzer showed a significant 20% decrease of Glu in ARO group in comparison to CTL. ChT was significantly higher in MIX and PLC in relation to both CTL and ARO groups, which arose mainly from LDL elevations. Also, there was a certain (30%), but insignificant increase of TG in MIX and PLC vs. CTL. The effects of aronia on transaminases were registered only for ALT, whose level in ARO was about 3-fold of that in CTL group. In conclusion, everyday free drinking of flavonoid-rich aronia juice solutions appeared to have global hypoglycemic effect in normal rats and, also, certain hypolipidemic effects, when hyperlipidemia was induced probably with higher sugar consumption.",
publisher = "Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6340"
}

Ignjatović, Đ., Tovilović-Kovačević, G., Tomić, M., Ranković, S., Popović, T., Debeljak Martačić, S.,& Glibetić, M.. (2015). Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”., 78-78.
https://hdl.handle.net/21.15107/rcub_ibiss_6340

Ignjatović Đ, Tovilović-Kovačević G, Tomić M, Ranković S, Popović T, Debeljak Martačić S, Glibetić M. Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:78-78.
https://hdl.handle.net/21.15107/rcub_ibiss_6340 .

Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Tomić, Mirko, Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Slavica, Glibetić, Marija, "Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in normal rats" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):78-78,
https://hdl.handle.net/21.15107/rcub_ibiss_6340 .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6348
AB  - Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.
PB  - Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology
C3  - Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
T1  - Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6348
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Marija",
year = "2015",
abstract = "Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.",
publisher = "Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology",
journal = "Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia",
title = "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6348"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2015). Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology., 85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348

Ranković S, Popović T, Debeljak Martačić J, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia. 2015;:85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Marija, "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats" in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia (2015):85-85,
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .

TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Petković, Branka
AU  - Milošević, Verica
AU  - Tomić, Mirko
PY  - 2012
UR  - http://www.chalcogen.ro/index.php/journals/digest-journal-of-nanomaterials-and-biostructures/8-djnb/29-volume-7-number-4-october-december-2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3782
AB  - Pharmaceutical preparations of Vitex agnus castus L. (VAC) were shown to contain several bioactive compounds, which were capable to interfere with action of specific human hormones, thus exerting putative beneficial therapeutic effects. The goal of this study was to examine the effects of the essential oil from berries of VAC on the motor behavior of male rat, in relation to the possible dopaminergic actions of the active components. Middle-aged male Wistar rats were treated with VAC essential oil for 3 weeks (60 mg/kg/day s.c) and submitted to the open field test. Their brain preparations were used for in vitro binding assays at dopamine receptors and immunohistochemical/morphometric analyses of pituitary lactotrophs. Chronic application of VAC essential oil significantly reduced rat motor activity, although it did not influence in vitro binding parameters at nigrostriatal D-1 and D-2 dopamine receptors. However, the relative volume density of prolactin-immunopositive cells per pituitary unit was significantly decreased by 36%, which reflected down-regulation of their function following the treatment. These findings suggest that the reduction in rat motor activity, registered after chronic administration of VAC essential oil, is probably induced by non-dopaminergic mechanism(s).
PB  - National Institute of Material Physics, Romania
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Bioactivity of the essential oil from berries of Vitex agnus castus in middle aged male rats
IS  - 4
VL  - 7
SP  - 1727
EP  - 1734
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3782
ER  - 

@article{
author = "Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Šošić-Jurjević, Branka  and Filipović, Branko and Petković, Branka and Milošević, Verica and Tomić, Mirko",
year = "2012",
abstract = "Pharmaceutical preparations of Vitex agnus castus L. (VAC) were shown to contain several bioactive compounds, which were capable to interfere with action of specific human hormones, thus exerting putative beneficial therapeutic effects. The goal of this study was to examine the effects of the essential oil from berries of VAC on the motor behavior of male rat, in relation to the possible dopaminergic actions of the active components. Middle-aged male Wistar rats were treated with VAC essential oil for 3 weeks (60 mg/kg/day s.c) and submitted to the open field test. Their brain preparations were used for in vitro binding assays at dopamine receptors and immunohistochemical/morphometric analyses of pituitary lactotrophs. Chronic application of VAC essential oil significantly reduced rat motor activity, although it did not influence in vitro binding parameters at nigrostriatal D-1 and D-2 dopamine receptors. However, the relative volume density of prolactin-immunopositive cells per pituitary unit was significantly decreased by 36%, which reflected down-regulation of their function following the treatment. These findings suggest that the reduction in rat motor activity, registered after chronic administration of VAC essential oil, is probably induced by non-dopaminergic mechanism(s).",
publisher = "National Institute of Material Physics, Romania",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Bioactivity of the essential oil from berries of Vitex agnus castus in middle aged male rats",
number = "4",
volume = "7",
pages = "1727-1734",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3782"
}

Ignjatović, Đ., Tovilović-Kovačević, G., Šošić-Jurjević, B., Filipović, B., Petković, B., Milošević, V.,& Tomić, M.. (2012). Bioactivity of the essential oil from berries of Vitex agnus castus in middle aged male rats. in Digest Journal of Nanomaterials and Biostructures
National Institute of Material Physics, Romania., 7(4), 1727-1734.
https://hdl.handle.net/21.15107/rcub_ibiss_3782

Ignjatović Đ, Tovilović-Kovačević G, Šošić-Jurjević B, Filipović B, Petković B, Milošević V, Tomić M. Bioactivity of the essential oil from berries of Vitex agnus castus in middle aged male rats. in Digest Journal of Nanomaterials and Biostructures. 2012;7(4):1727-1734.
https://hdl.handle.net/21.15107/rcub_ibiss_3782 .

Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Šošić-Jurjević, Branka , Filipović, Branko, Petković, Branka, Milošević, Verica, Tomić, Mirko, "Bioactivity of the essential oil from berries of Vitex agnus castus in middle aged male rats" in Digest Journal of Nanomaterials and Biostructures, 7, no. 4 (2012):1727-1734,
https://hdl.handle.net/21.15107/rcub_ibiss_3782 .

TY  - JOUR
AU  - Tovilović-Kovačević, Gordana
AU  - Krstić Milošević, Dijana
AU  - Ignjatović, Đurđica
AU  - Petković, Branka
AU  - Tomić, Mirko
PY  - 2011
UR  - http://www.chalcogen.ro/index.php/journals/digest-journal-of-nanomaterials-and-biostructures/8-djnb/24-volume-6-number-3-july-september-2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3800
AB  - A diethylether extract of Gentiana kochiana aerial parts (GE), rich in two xanthones (gentiacaulein and gentiakochianin, 90% w/w), has already been shown to inhibit microsomal MAO enzymes in vitro and to exhibit certain antidepressant effects on mice. The aim of this study is to evaluate anxiolytic-like activity of GE in rodents by three behavioral tests, and to explore in vitro interactions of GE/xanthones with some anxiety-related elements of neurotransmission. The elevated plus maze test in rats and the staircase test in mice reveal specific anxiolytic-like influences of GE (5-20(40) mg/kg; ip), with the dose-effect ratios mainly converging to the inverted U-shaped curve. These effects are only partially analogous to the action of anxiolytic diazepam. In vitro assays demonstrate that neither GE nor the xanthones express substantial interactions with alpha(1)-adrenergic receptors, GABA(A) and GABA(B) receptors, and acetylcholine esterase. These results suggest a specific anxiolytic-like potential of GE in rodents and reduce the probability of particular neurochemical mechanisms to underlie this action.
PB  - National Institute of Material Physics, Romania
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Anxiolytic-like effects of xanthone-rich diethylether extract of Gentiana kochiana in rodents
IS  - 3
VL  - 6
SP  - 1385
EP  - 1392
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3800
ER  - 

@article{
author = "Tovilović-Kovačević, Gordana and Krstić Milošević, Dijana and Ignjatović, Đurđica and Petković, Branka and Tomić, Mirko",
year = "2011",
abstract = "A diethylether extract of Gentiana kochiana aerial parts (GE), rich in two xanthones (gentiacaulein and gentiakochianin, 90% w/w), has already been shown to inhibit microsomal MAO enzymes in vitro and to exhibit certain antidepressant effects on mice. The aim of this study is to evaluate anxiolytic-like activity of GE in rodents by three behavioral tests, and to explore in vitro interactions of GE/xanthones with some anxiety-related elements of neurotransmission. The elevated plus maze test in rats and the staircase test in mice reveal specific anxiolytic-like influences of GE (5-20(40) mg/kg; ip), with the dose-effect ratios mainly converging to the inverted U-shaped curve. These effects are only partially analogous to the action of anxiolytic diazepam. In vitro assays demonstrate that neither GE nor the xanthones express substantial interactions with alpha(1)-adrenergic receptors, GABA(A) and GABA(B) receptors, and acetylcholine esterase. These results suggest a specific anxiolytic-like potential of GE in rodents and reduce the probability of particular neurochemical mechanisms to underlie this action.",
publisher = "National Institute of Material Physics, Romania",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Anxiolytic-like effects of xanthone-rich diethylether extract of Gentiana kochiana in rodents",
number = "3",
volume = "6",
pages = "1385-1392",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3800"
}

Tovilović-Kovačević, G., Krstić Milošević, D., Ignjatović, Đ., Petković, B.,& Tomić, M.. (2011). Anxiolytic-like effects of xanthone-rich diethylether extract of Gentiana kochiana in rodents. in Digest Journal of Nanomaterials and Biostructures
National Institute of Material Physics, Romania., 6(3), 1385-1392.
https://hdl.handle.net/21.15107/rcub_ibiss_3800

Tovilović-Kovačević G, Krstić Milošević D, Ignjatović Đ, Petković B, Tomić M. Anxiolytic-like effects of xanthone-rich diethylether extract of Gentiana kochiana in rodents. in Digest Journal of Nanomaterials and Biostructures. 2011;6(3):1385-1392.
https://hdl.handle.net/21.15107/rcub_ibiss_3800 .

Tovilović-Kovačević, Gordana, Krstić Milošević, Dijana, Ignjatović, Đurđica, Petković, Branka, Tomić, Mirko, "Anxiolytic-like effects of xanthone-rich diethylether extract of Gentiana kochiana in rodents" in Digest Journal of Nanomaterials and Biostructures, 6, no. 3 (2011):1385-1392,
https://hdl.handle.net/21.15107/rcub_ibiss_3800 .