TY  - JOUR
AU  - Batos, Branislava
AU  - Miljković, Danijela
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0534-00121901121B
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3370
AB  - The variability of morphological traits of Picea omorika /Panč./Purkyne pollen was analyzed in two successive years on the pollen sampled from 24 trees in a seed stand at the site of Bela Zemlja in the area of Mt. Zlatibor (Serbia). The aim of the research was to obtain the index of phenotypic plasticity of the equatorial and polar axes and the coefficient of shape of pollen. According to the obtained results, Serbian spruce pollen grains are 93.3 μm/53.2 μm in size with their shape being oblate 57.5%. The climate characteristics of the study years initiated different responses of the pollen traits for each tree. Reaction norms were slightly steep and they crossed as confirmed by the significant interaction between the variability factors of the year and the tree. In the year with lower temperatures and less precipitation, the mean values of the equatorial axis were higher and the polar axis smaller, giving the pollen grain a more oblate form. According to the results of the applied model of analysis of variance where the year and the tree, as well as the interaction between them, were taken as factors of variability, the values of the pollen equatorial axis (length) and the coefficient of shape showed statistically significant differences between the years. Genetic variability (interindividual differences) for the values of both axes of pollen grains was confirmed by statistically significant differences between the trees in a single year. Interindividual differences in the observed morphological pollen traits were affected by environmental conditions specific to the year of sampling (statistically significant year x tree interaction). All the analyzed traits showed interindividual differences in the values of the plasticity index. The lowest values of plasticity were obtained for the equatorial axis (length) of the pollen grain, and they were significantly different from the plasticity indexes of the other traits analyzed herein. The plasticity of pollen traits has an important role in the controlled selection and breeding of species with the aim of obtaining more resistant genotypes with a greater ability to adapt to the fluctuations of environmental factors caused by global climate change.
T2  - Genetika
T2  - Genetika
T1  - The phenotypic plasticity of of Picea omorika /Panc./Purkyne morphological pollen traits
IS  - 1
VL  - 51
DO  - 10.2298/GENSR1901121B
SP  - 121
EP  - 136
ER  - 

@article{
author = "Batos, Branislava and Miljković, Danijela",
year = "2019",
abstract = "The variability of morphological traits of Picea omorika /Panč./Purkyne pollen was analyzed in two successive years on the pollen sampled from 24 trees in a seed stand at the site of Bela Zemlja in the area of Mt. Zlatibor (Serbia). The aim of the research was to obtain the index of phenotypic plasticity of the equatorial and polar axes and the coefficient of shape of pollen. According to the obtained results, Serbian spruce pollen grains are 93.3 μm/53.2 μm in size with their shape being oblate 57.5%. The climate characteristics of the study years initiated different responses of the pollen traits for each tree. Reaction norms were slightly steep and they crossed as confirmed by the significant interaction between the variability factors of the year and the tree. In the year with lower temperatures and less precipitation, the mean values of the equatorial axis were higher and the polar axis smaller, giving the pollen grain a more oblate form. According to the results of the applied model of analysis of variance where the year and the tree, as well as the interaction between them, were taken as factors of variability, the values of the pollen equatorial axis (length) and the coefficient of shape showed statistically significant differences between the years. Genetic variability (interindividual differences) for the values of both axes of pollen grains was confirmed by statistically significant differences between the trees in a single year. Interindividual differences in the observed morphological pollen traits were affected by environmental conditions specific to the year of sampling (statistically significant year x tree interaction). All the analyzed traits showed interindividual differences in the values of the plasticity index. The lowest values of plasticity were obtained for the equatorial axis (length) of the pollen grain, and they were significantly different from the plasticity indexes of the other traits analyzed herein. The plasticity of pollen traits has an important role in the controlled selection and breeding of species with the aim of obtaining more resistant genotypes with a greater ability to adapt to the fluctuations of environmental factors caused by global climate change.",
journal = "Genetika, Genetika",
title = "The phenotypic plasticity of of Picea omorika /Panc./Purkyne morphological pollen traits",
number = "1",
volume = "51",
doi = "10.2298/GENSR1901121B",
pages = "121-136"
}

Batos, B.,& Miljković, D.. (2019). The phenotypic plasticity of of Picea omorika /Panc./Purkyne morphological pollen traits. in Genetika, 51(1), 121-136.
https://doi.org/10.2298/GENSR1901121B

Batos B, Miljković D. The phenotypic plasticity of of Picea omorika /Panc./Purkyne morphological pollen traits. in Genetika. 2019;51(1):121-136.
doi:10.2298/GENSR1901121B .

Batos, Branislava, Miljković, Danijela, "The phenotypic plasticity of of Picea omorika /Panc./Purkyne morphological pollen traits" in Genetika, 51, no. 1 (2019):121-136,
https://doi.org/10.2298/GENSR1901121B . .

TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanović, Vladimir
AU  - Brkić, Dejan
AU  - Manojlović Stojanoski, Milica
AU  - Milošević, Verica
AU  - Sekulić, Milka
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6131
AB  - Thyroid C cells hormone, calcitonine, inhibits bone resorption. We have demonstrated that daidzein treatment of orchidectomized rats (model for osteoporosis) stimulated C cells and increased trabecular bone mass. These results suggest that, besides direct action, daidzein may also affect bone structure indirectly through enhancement of thyroid C cell activity. Thyroid C cells produce calcitonin (CT) which acts as an inhibitor of bone resorption. In this study, the influence of daidzein treatment on thyroid C cells, bone structure, and bone function in orchidectomized (Orx) middle-aged rats was investigated. Sixteen-month-old Wistar rats were divided into Orx and sham-operated (SO) groups. Half the Orx rats were given subcutaneous injections of daidzein (30 mg/kg b.w./day) for 3 weeks. CT-immunopositive thyroid C cells were morphometrically analyzed. The metaphyseal region of the proximal tibia was measured histomorphometrically, and cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were calculated. Serum samples were analyzed for CT and osteocalcin (OC), calcium (Ca) and phosphorus concentrations, and urine samples for Ca levels. Treatment of Orx animals with daidzein significantly increased volume of C cells compared to the Orx rats. Daidzein also enhanced B.Ar, Tb.Th, and Tb.N and reduced Tb.Sp. The serum OC and urinary Ca concentrations decreased significantly in comparison with the Orx group. These findings indicate that daidzein treatment stimulates thyroid C cells, increase trabecular bone mass, and decrease bone turnover in Orx middle-aged rats, which is the model of male osteoporosis.
PB  - Springer Nature
T2  - Osteoporosis International
T1  - Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats
IS  - 9
VL  - 21
DO  - 10.1007/s00198-009-1092-x
SP  - 1609
EP  - 1616
ER  - 

@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanović, Vladimir and Brkić, Dejan and Manojlović Stojanoski, Milica and Milošević, Verica and Sekulić, Milka",
year = "2010",
abstract = "Thyroid C cells hormone, calcitonine, inhibits bone resorption. We have demonstrated that daidzein treatment of orchidectomized rats (model for osteoporosis) stimulated C cells and increased trabecular bone mass. These results suggest that, besides direct action, daidzein may also affect bone structure indirectly through enhancement of thyroid C cell activity. Thyroid C cells produce calcitonin (CT) which acts as an inhibitor of bone resorption. In this study, the influence of daidzein treatment on thyroid C cells, bone structure, and bone function in orchidectomized (Orx) middle-aged rats was investigated. Sixteen-month-old Wistar rats were divided into Orx and sham-operated (SO) groups. Half the Orx rats were given subcutaneous injections of daidzein (30 mg/kg b.w./day) for 3 weeks. CT-immunopositive thyroid C cells were morphometrically analyzed. The metaphyseal region of the proximal tibia was measured histomorphometrically, and cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were calculated. Serum samples were analyzed for CT and osteocalcin (OC), calcium (Ca) and phosphorus concentrations, and urine samples for Ca levels. Treatment of Orx animals with daidzein significantly increased volume of C cells compared to the Orx rats. Daidzein also enhanced B.Ar, Tb.Th, and Tb.N and reduced Tb.Sp. The serum OC and urinary Ca concentrations decreased significantly in comparison with the Orx group. These findings indicate that daidzein treatment stimulates thyroid C cells, increase trabecular bone mass, and decrease bone turnover in Orx middle-aged rats, which is the model of male osteoporosis.",
publisher = "Springer Nature",
journal = "Osteoporosis International",
title = "Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats",
number = "9",
volume = "21",
doi = "10.1007/s00198-009-1092-x",
pages = "1609-1616"
}

Filipović, B., Šošić-Jurjević, B., Ajdžanović, V., Brkić, D., Manojlović Stojanoski, M., Milošević, V.,& Sekulić, M.. (2010). Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats. in Osteoporosis International
Springer Nature., 21(9), 1609-1616.
https://doi.org/10.1007/s00198-009-1092-x

Filipović B, Šošić-Jurjević B, Ajdžanović V, Brkić D, Manojlović Stojanoski M, Milošević V, Sekulić M. Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats. in Osteoporosis International. 2010;21(9):1609-1616.
doi:10.1007/s00198-009-1092-x .

Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanović, Vladimir, Brkić, Dejan, Manojlović Stojanoski, Milica, Milošević, Verica, Sekulić, Milka, "Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats" in Osteoporosis International, 21, no. 9 (2010):1609-1616,
https://doi.org/10.1007/s00198-009-1092-x . .

TY  - JOUR
AU  - Milošević, Verica
AU  - Nestorović, Nataša
AU  - Terzić, Milica
AU  - Ristić, Nataša
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Sekulić, Milka
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6053
AB  - The prolonged effects of neonatal SRIH-14 treatment on pituitary ACTH cells were investigated. Neonatal female rats were injected subcutaneously with SRIH (20 μg/100g b.w.) every 12 hours for five consecutive days (3rd-7th day of life). Groups of rats were then killed at the juvenile (16th day), peripubertal (38th day) or adult (80th day) stage. ACTH cells were visualized using the peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate the ACTH-immunoreactive cell volume and volume density. The histological and immunocytochemical characteristics of ACTH cells in neonatally treated females were changed in all examined periods. Thus, SRIH-14 induced significant (p<0.05) decreases of ACTH cell volume in juvenile, peripubertal and adult rats by 26%, 39% and 14%, respectively, in comparison to the corresponding controls. The volume density of ACTH cells was also diminished (by 31%; p<0.05) at the juvenile stage in comparison with the corresponding controls. In peripubertal and adult rats, the volume densities of ACTH cells were somewhat lower (17% and 14%, respectively), but the decreases did not reach statistical significance. These findings suggest that neonatal treatment with SRIH-14 exerts a marked prolonged inhibitory effect on ACTH cell
morphology extending to the adult period of life.
PB  - VIA MEDICA
T2  - Folia Histochemica et Cytobiologica
T1  - Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14
IS  - 3
VL  - 47
DO  - 10.2478/v10042-009-0104-1
SP  - 479
EP  - 484
ER  - 

@article{
author = "Milošević, Verica and Nestorović, Nataša and Terzić, Milica and Ristić, Nataša and Ajdžanović, Vladimir and Trifunović, Svetlana and Sekulić, Milka",
year = "2009",
abstract = "The prolonged effects of neonatal SRIH-14 treatment on pituitary ACTH cells were investigated. Neonatal female rats were injected subcutaneously with SRIH (20 μg/100g b.w.) every 12 hours for five consecutive days (3rd-7th day of life). Groups of rats were then killed at the juvenile (16th day), peripubertal (38th day) or adult (80th day) stage. ACTH cells were visualized using the peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate the ACTH-immunoreactive cell volume and volume density. The histological and immunocytochemical characteristics of ACTH cells in neonatally treated females were changed in all examined periods. Thus, SRIH-14 induced significant (p<0.05) decreases of ACTH cell volume in juvenile, peripubertal and adult rats by 26%, 39% and 14%, respectively, in comparison to the corresponding controls. The volume density of ACTH cells was also diminished (by 31%; p<0.05) at the juvenile stage in comparison with the corresponding controls. In peripubertal and adult rats, the volume densities of ACTH cells were somewhat lower (17% and 14%, respectively), but the decreases did not reach statistical significance. These findings suggest that neonatal treatment with SRIH-14 exerts a marked prolonged inhibitory effect on ACTH cell
morphology extending to the adult period of life.",
publisher = "VIA MEDICA",
journal = "Folia Histochemica et Cytobiologica",
title = "Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14",
number = "3",
volume = "47",
doi = "10.2478/v10042-009-0104-1",
pages = "479-484"
}

Milošević, V., Nestorović, N., Terzić, M., Ristić, N., Ajdžanović, V., Trifunović, S.,& Sekulić, M.. (2009). Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14. in Folia Histochemica et Cytobiologica
VIA MEDICA., 47(3), 479-484.
https://doi.org/10.2478/v10042-009-0104-1

Milošević V, Nestorović N, Terzić M, Ristić N, Ajdžanović V, Trifunović S, Sekulić M. Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14. in Folia Histochemica et Cytobiologica. 2009;47(3):479-484.
doi:10.2478/v10042-009-0104-1 .

Milošević, Verica, Nestorović, Nataša, Terzić, Milica, Ristić, Nataša, Ajdžanović, Vladimir, Trifunović, Svetlana, Sekulić, Milka, "Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14" in Folia Histochemica et Cytobiologica, 47, no. 3 (2009):479-484,
https://doi.org/10.2478/v10042-009-0104-1 . .

TY  - JOUR
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Ristić, Nataša
AU  - Sekulić, Milka
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2008
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5863
AB  - The effects of multiple somatostatin (SRIH-14) administration on the pituitary–ovarian axis were examined in peripubertal rats. Female Wistar rats received subcutaneously, two daily doses of 20 μg SRIH-14 per 100 g body weight (b.w.) for five consecutive days (from the 33rd to the 37th day of life). Follicle-stimulating (FSH), luteinizing (LH) and somatotropic (GH) cells were examined by the peroxidase–anti-peroxidase immunocytochemical method. Changes in cell volumes, volume densities and number per unit area (mm2) of FSH-, LH- and GH-immunoreactive cells were evaluated by stereology and morphometry. Serum FSH and LH levels were determined by RIA. Ovaries were analyzed by simple point counting of follicles. The volumes and volume densities of FSH-, LH- and GH-immunoreactive cells were significantly decreased while their numbers per mm2 remained unchanged. SRIH-14 induced a significant decrease in serum FSH and LH levels. In the ovary, SRIH-14 induced an increase in the number of primordial follicles, followed by a reduction in the number of small healthy growing follicles and absence of preovulatory follicles. The number of atretic follicles was unchanged. We concluded that treatment with SRIH-14 during the peripubertal period markedly inhibited pituitary FSH, LH and GH cells. In the ovary, SRIH-14 acted by inhibiting folliculogenesis without affecting atretic processes.
PB  - Springer Nature
T2  - Histochemistry and Cell Biology
T1  - Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats
VL  - 130
DO  - 10.1007/s00418-008-0442-7
SP  - 699
EP  - 708
ER  - 

@article{
author = "Nestorović, Nataša and Manojlović-Stojanoski, Milica and Ristić, Nataša and Sekulić, Milka and Šošić-Jurjević, Branka  and Filipović, Branko and Milošević, Verica",
year = "2008",
abstract = "The effects of multiple somatostatin (SRIH-14) administration on the pituitary–ovarian axis were examined in peripubertal rats. Female Wistar rats received subcutaneously, two daily doses of 20 μg SRIH-14 per 100 g body weight (b.w.) for five consecutive days (from the 33rd to the 37th day of life). Follicle-stimulating (FSH), luteinizing (LH) and somatotropic (GH) cells were examined by the peroxidase–anti-peroxidase immunocytochemical method. Changes in cell volumes, volume densities and number per unit area (mm2) of FSH-, LH- and GH-immunoreactive cells were evaluated by stereology and morphometry. Serum FSH and LH levels were determined by RIA. Ovaries were analyzed by simple point counting of follicles. The volumes and volume densities of FSH-, LH- and GH-immunoreactive cells were significantly decreased while their numbers per mm2 remained unchanged. SRIH-14 induced a significant decrease in serum FSH and LH levels. In the ovary, SRIH-14 induced an increase in the number of primordial follicles, followed by a reduction in the number of small healthy growing follicles and absence of preovulatory follicles. The number of atretic follicles was unchanged. We concluded that treatment with SRIH-14 during the peripubertal period markedly inhibited pituitary FSH, LH and GH cells. In the ovary, SRIH-14 acted by inhibiting folliculogenesis without affecting atretic processes.",
publisher = "Springer Nature",
journal = "Histochemistry and Cell Biology",
title = "Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats",
volume = "130",
doi = "10.1007/s00418-008-0442-7",
pages = "699-708"
}

Nestorović, N., Manojlović-Stojanoski, M., Ristić, N., Sekulić, M., Šošić-Jurjević, B., Filipović, B.,& Milošević, V.. (2008). Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats. in Histochemistry and Cell Biology
Springer Nature., 130, 699-708.
https://doi.org/10.1007/s00418-008-0442-7

Nestorović N, Manojlović-Stojanoski M, Ristić N, Sekulić M, Šošić-Jurjević B, Filipović B, Milošević V. Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats. in Histochemistry and Cell Biology. 2008;130:699-708.
doi:10.1007/s00418-008-0442-7 .

Nestorović, Nataša, Manojlović-Stojanoski, Milica, Ristić, Nataša, Sekulić, Milka, Šošić-Jurjević, Branka , Filipović, Branko, Milošević, Verica, "Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats" in Histochemistry and Cell Biology, 130 (2008):699-708,
https://doi.org/10.1007/s00418-008-0442-7 . .

TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Manojlović-Stojanoski, Milica
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Milošević, Verica
AU  - Sekulić, Milka
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6103
AB  - This study was to evaluate the effect of androgen deficiency on thyroid immunoreactive C-cells and bone structure and function in a male orchidectomized middle-aged rat model. Fifteen-month-old male Wistar rats were divided into orchidectomized (Orx) and the sham-operated control (Sham) group. In the Orx group significant decreases (P < 0.05) were found in the volume of C cells (by 14%), their relative volume density (by 13%) and serum calcitonin concentration (by 54%) compared to the controls. Analyses of trabecular microarchitecture of the proximal tibia metaphysis showed that Orx induced marked decreases of cancellous bone area, trabecular thickness and trabecular number (by 52, 20 and 19% respectively; P < 0.05), whereas trabecular separation was increased by 27% (P < 0.05). In Orx rats, serum osteocalcin concentration was increased by 119% (P < 0.05), while serum calcium and phosphorus were 6 and 14% (P < 0.05) lower, respectively, compared to the levels in the Sham. In addition, urine calcium content was considerably higher (by 129%; P < 0.05) in Orx animals. These findings indicate that the androgen deficiency caused by Orx in middle-aged rats modulated the structure of C cells and diminished secretion of calcitonin. Histomorphometrical and biochemical analyses demonstrated a decrease of cancellous bone mass and increased bone turnover.
PB  - Heidelberg: Springer Nature
T2  - Histochemistry and Cell Biology
T1  - The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats
VL  - 128
DO  - 10.1007/s00418-007-0307-5
SP  - 153
EP  - 159
ER  - 

@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka  and Ajdžanović, Vladimir and Trifunović, Svetlana and Manojlović-Stojanoski, Milica and Ristić, Nataša and Nestorović, Nataša and Milošević, Verica and Sekulić, Milka",
year = "2007",
abstract = "This study was to evaluate the effect of androgen deficiency on thyroid immunoreactive C-cells and bone structure and function in a male orchidectomized middle-aged rat model. Fifteen-month-old male Wistar rats were divided into orchidectomized (Orx) and the sham-operated control (Sham) group. In the Orx group significant decreases (P < 0.05) were found in the volume of C cells (by 14%), their relative volume density (by 13%) and serum calcitonin concentration (by 54%) compared to the controls. Analyses of trabecular microarchitecture of the proximal tibia metaphysis showed that Orx induced marked decreases of cancellous bone area, trabecular thickness and trabecular number (by 52, 20 and 19% respectively; P < 0.05), whereas trabecular separation was increased by 27% (P < 0.05). In Orx rats, serum osteocalcin concentration was increased by 119% (P < 0.05), while serum calcium and phosphorus were 6 and 14% (P < 0.05) lower, respectively, compared to the levels in the Sham. In addition, urine calcium content was considerably higher (by 129%; P < 0.05) in Orx animals. These findings indicate that the androgen deficiency caused by Orx in middle-aged rats modulated the structure of C cells and diminished secretion of calcitonin. Histomorphometrical and biochemical analyses demonstrated a decrease of cancellous bone mass and increased bone turnover.",
publisher = "Heidelberg: Springer Nature",
journal = "Histochemistry and Cell Biology",
title = "The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats",
volume = "128",
doi = "10.1007/s00418-007-0307-5",
pages = "153-159"
}

Filipović, B., Šošić-Jurjević, B., Ajdžanović, V., Trifunović, S., Manojlović-Stojanoski, M., Ristić, N., Nestorović, N., Milošević, V.,& Sekulić, M.. (2007). The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats. in Histochemistry and Cell Biology
Heidelberg: Springer Nature., 128, 153-159.
https://doi.org/10.1007/s00418-007-0307-5

Filipović B, Šošić-Jurjević B, Ajdžanović V, Trifunović S, Manojlović-Stojanoski M, Ristić N, Nestorović N, Milošević V, Sekulić M. The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats. in Histochemistry and Cell Biology. 2007;128:153-159.
doi:10.1007/s00418-007-0307-5 .

Filipović, Branko, Šošić-Jurjević, Branka , Ajdžanović, Vladimir, Trifunović, Svetlana, Manojlović-Stojanoski, Milica, Ristić, Nataša, Nestorović, Nataša, Milošević, Verica, Sekulić, Milka, "The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats" in Histochemistry and Cell Biology, 128 (2007):153-159,
https://doi.org/10.1007/s00418-007-0307-5 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Brkić, Dejan
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Sekulić, Milka
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6099
AB  - Nutritional supplements containing soybean phytoestrogens, the isoflavones genistein (G) and daidzein (D), are increasingly used as alternative therapy for osteoporosis, cancer, and cardiovascular and other diseases with a frequency that increases with advancing age. In this study we examined the effects of subcutaneous administration of either G or D on serum lipid levels in orchidectomized (Orx) and intact (IA) middle-aged male rats, which are experimental models of andropause. Sixteen-month-old Wistar rats were treated with 10 mg/kg and 30 mg/kg of either G or D. The control groups received testosterone, estradiol, or vehicle for 3 weeks, after which the total serum cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), and total triglycerides (TT) were measured. Compared with the matching vehicle-treated controls, the higher doses of G and D and testosterone treatment significantly (P < 0.05) lowered the TC and lipoprotein cholesterol levels. The greatest effect was observed regarding LDL-C in both Orx and IA males after G and D treatments, in which LDL-C decreased by more than 30%. The lower isoflavone doses induced a significant cholesterol-lowering effect (P < 0.05) only in the Orx group. Like the estradiol treatment, the higher doses of G and D increased the TT levels in both rat models by more than 50% (P < 0.05). The lower doses of isoflavones increased TT only in the Orx group. In male middle-aged rats, injections of higher doses of G and D decreased the serum cholesterol levels, as did testosterone injection, and brought about an increase in serum triglycerides similar to that observed after estradiol treatment.
PB  - SAGE Publications
T2  - Experimental biology and medicine (Maywood, N.J.)
T1  - Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats
IS  - 9
VL  - 232
DO  - 10.3181/0703-BC-82
SP  - 1222
EP  - 1227
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Filipović, Branko and Ajdžanović, Vladimir and Brkić, Dejan and Ristić, Nataša and Manojlović-Stojanoski, Milica and Nestorović, Nataša and Trifunović, Svetlana and Sekulić, Milka",
year = "2007",
abstract = "Nutritional supplements containing soybean phytoestrogens, the isoflavones genistein (G) and daidzein (D), are increasingly used as alternative therapy for osteoporosis, cancer, and cardiovascular and other diseases with a frequency that increases with advancing age. In this study we examined the effects of subcutaneous administration of either G or D on serum lipid levels in orchidectomized (Orx) and intact (IA) middle-aged male rats, which are experimental models of andropause. Sixteen-month-old Wistar rats were treated with 10 mg/kg and 30 mg/kg of either G or D. The control groups received testosterone, estradiol, or vehicle for 3 weeks, after which the total serum cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), and total triglycerides (TT) were measured. Compared with the matching vehicle-treated controls, the higher doses of G and D and testosterone treatment significantly (P < 0.05) lowered the TC and lipoprotein cholesterol levels. The greatest effect was observed regarding LDL-C in both Orx and IA males after G and D treatments, in which LDL-C decreased by more than 30%. The lower isoflavone doses induced a significant cholesterol-lowering effect (P < 0.05) only in the Orx group. Like the estradiol treatment, the higher doses of G and D increased the TT levels in both rat models by more than 50% (P < 0.05). The lower doses of isoflavones increased TT only in the Orx group. In male middle-aged rats, injections of higher doses of G and D decreased the serum cholesterol levels, as did testosterone injection, and brought about an increase in serum triglycerides similar to that observed after estradiol treatment.",
publisher = "SAGE Publications",
journal = "Experimental biology and medicine (Maywood, N.J.)",
title = "Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats",
number = "9",
volume = "232",
doi = "10.3181/0703-BC-82",
pages = "1222-1227"
}

Šošić-Jurjević, B., Filipović, B., Ajdžanović, V., Brkić, D., Ristić, N., Manojlović-Stojanoski, M., Nestorović, N., Trifunović, S.,& Sekulić, M.. (2007). Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats. in Experimental biology and medicine (Maywood, N.J.)
SAGE Publications., 232(9), 1222-1227.
https://doi.org/10.3181/0703-BC-82

Šošić-Jurjević B, Filipović B, Ajdžanović V, Brkić D, Ristić N, Manojlović-Stojanoski M, Nestorović N, Trifunović S, Sekulić M. Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats. in Experimental biology and medicine (Maywood, N.J.). 2007;232(9):1222-1227.
doi:10.3181/0703-BC-82 .

Šošić-Jurjević, Branka , Filipović, Branko, Ajdžanović, Vladimir, Brkić, Dejan, Ristić, Nataša, Manojlović-Stojanoski, Milica, Nestorović, Nataša, Trifunović, Svetlana, Sekulić, Milka, "Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats" in Experimental biology and medicine (Maywood, N.J.), 232, no. 9 (2007):1222-1227,
https://doi.org/10.3181/0703-BC-82 . .

TY  - JOUR
AU  - Sekulić, Milka
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Milošević, Verica
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6098
AB  - The cellular and subcellular organization of thyroid follicular cells in peripubertal (6-month-old) male pigs treated with estradiol-dipropionate (Oe) plus progesterone (Pr) in combination on the first postnatal day was studied. A stereological method was used for morphometric determinations of the relative volume densities of the follicular epithelium, colloid and interstitium, and for establishing the epithelial height and index of activation rate. Statistically significant differences of the examined parameters between the control and Oe+Pr -treated groups were determined by Student's t-test. The subcellular organization of thyroid follicular cells was studied using transmission electron microscopy (TEM). When compared with the control group, in the Oe+Pr -treated pigs, thyroid follicles possessed a noticeably higher follicular epithelium when compared with the control animals. The observed changes were quantified and comparison between the experimental groups showed that the height, volume density of follicular epithelium, and index of activation rate were significantly (P < 0.05) increased, whereas the relative volume density of the colloid was significantly (P < 0.05) decreased. At the subcellular level the thyroid follicular cells of Oe+Pr -treated pigs were characterized by increased number of polysomes and dense bodies and extensive endoplasmatic reticulum. It was concluded that a single neonatal treatment with female gonadal steroids exerted a prolonged effect on the pig's thyroid, characterized by increased biosynthesis and reabsorption of the colloid by the follicular cells.
PB  - Hoboken: Wiley
T2  - Microscopy Research and Technique
T1  - Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study
VL  - 70
DO  - 10.1002/jemt.20384
SP  - 44
EP  - 49
ER  - 

@article{
author = "Sekulić, Milka and Šošić-Jurjević, Branka and Filipović, Branko and Nestorović, Nataša and Ristić, Nataša and Manojlović-Stojanoski, Milica and Milošević, Verica",
year = "2007",
abstract = "The cellular and subcellular organization of thyroid follicular cells in peripubertal (6-month-old) male pigs treated with estradiol-dipropionate (Oe) plus progesterone (Pr) in combination on the first postnatal day was studied. A stereological method was used for morphometric determinations of the relative volume densities of the follicular epithelium, colloid and interstitium, and for establishing the epithelial height and index of activation rate. Statistically significant differences of the examined parameters between the control and Oe+Pr -treated groups were determined by Student's t-test. The subcellular organization of thyroid follicular cells was studied using transmission electron microscopy (TEM). When compared with the control group, in the Oe+Pr -treated pigs, thyroid follicles possessed a noticeably higher follicular epithelium when compared with the control animals. The observed changes were quantified and comparison between the experimental groups showed that the height, volume density of follicular epithelium, and index of activation rate were significantly (P < 0.05) increased, whereas the relative volume density of the colloid was significantly (P < 0.05) decreased. At the subcellular level the thyroid follicular cells of Oe+Pr -treated pigs were characterized by increased number of polysomes and dense bodies and extensive endoplasmatic reticulum. It was concluded that a single neonatal treatment with female gonadal steroids exerted a prolonged effect on the pig's thyroid, characterized by increased biosynthesis and reabsorption of the colloid by the follicular cells.",
publisher = "Hoboken: Wiley",
journal = "Microscopy Research and Technique",
title = "Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study",
volume = "70",
doi = "10.1002/jemt.20384",
pages = "44-49"
}

Sekulić, M., Šošić-Jurjević, B., Filipović, B., Nestorović, N., Ristić, N., Manojlović-Stojanoski, M.,& Milošević, V.. (2007). Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study. in Microscopy Research and Technique
Hoboken: Wiley., 70, 44-49.
https://doi.org/10.1002/jemt.20384

Sekulić M, Šošić-Jurjević B, Filipović B, Nestorović N, Ristić N, Manojlović-Stojanoski M, Milošević V. Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study. in Microscopy Research and Technique. 2007;70:44-49.
doi:10.1002/jemt.20384 .

Sekulić, Milka, Šošić-Jurjević, Branka, Filipović, Branko, Nestorović, Nataša, Ristić, Nataša, Manojlović-Stojanoski, Milica, Milošević, Verica, "Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study" in Microscopy Research and Technique, 70 (2007):44-49,
https://doi.org/10.1002/jemt.20384 . .