TY  - JOUR
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Paunović, Danijela
AU  - Giurato, Giorgio
AU  - Filipović, Biljana
AU  - Subotić, Angelina
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3686
AB  - Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis
IS  - 2
VL  - 141
DO  - 10.1007/s11240-020-01801-w
SP  - 417
EP  - 433
ER  - 

@article{
author = "Ćuković, Katarina and Dragićević, Milan and Bogdanović, Milica and Paunović, Danijela and Giurato, Giorgio and Filipović, Biljana and Subotić, Angelina and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis",
number = "2",
volume = "141",
doi = "10.1007/s11240-020-01801-w",
pages = "417-433"
}

Ćuković, K., Dragićević, M., Bogdanović, M., Paunović, D., Giurato, G., Filipović, B., Subotić, A., Todorović, S.,& Simonović, A.. (2020). Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture
Springer., 141(2), 417-433.
https://doi.org/10.1007/s11240-020-01801-w

Ćuković K, Dragićević M, Bogdanović M, Paunović D, Giurato G, Filipović B, Subotić A, Todorović S, Simonović A. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture. 2020;141(2):417-433.
doi:10.1007/s11240-020-01801-w .

Ćuković, Katarina, Dragićević, Milan, Bogdanović, Milica, Paunović, Danijela, Giurato, Giorgio, Filipović, Biljana, Subotić, Angelina, Todorović, Slađana, Simonović, Ana, "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis" in Plant Cell, Tissue and Organ Culture, 141, no. 2 (2020):417-433,
https://doi.org/10.1007/s11240-020-01801-w . .

TY  - CONF
AU  - Opačić, Miloš
AU  - Zorović, Maja
AU  - Savić, Danijela
AU  - Živin, Marko
AU  - Raičević, Savo
AU  - Baščarević, Vladimir
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5986
AB  - Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients
SP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5986
ER  - 

@conference{
author = "Opačić, Miloš and Zorović, Maja and Savić, Danijela and Živin, Marko and Raičević, Savo and Baščarević, Vladimir and Ristić, Aleksandar and Sokić, Dragoslav and Spasojević, Ivan",
year = "2019",
abstract = "Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5986"
}

Opačić, M., Zorović, M., Savić, D., Živin, M., Raičević, S., Baščarević, V., Ristić, A., Sokić, D.,& Spasojević, I.. (2019). Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986

Opačić M, Zorović M, Savić D, Živin M, Raičević S, Baščarević V, Ristić A, Sokić D, Spasojević I. Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

Opačić, Miloš, Zorović, Maja, Savić, Danijela, Živin, Marko, Raičević, Savo, Baščarević, Vladimir, Ristić, Aleksandar, Sokić, Dragoslav, Spasojević, Ivan, "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):296,
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Todorović, Slađana
AU  - Bogdanović, Milica
AU  - Simonović, Ana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5439
AB  - Centaurium erythraea Rafn. (Gentianaceae) represents a well-known medicinal plant rich in secondary metabolites and an appropriate model system for studying developmental biology due to its great developmental plasticity in vitro. One of the most unique manifestations of this characteristic in plants is somatic embryogenesis (SE), the development of embryos from somatic cells. SE initiation and progressing of the embryo through different developmental stages involves gene reprogramming and differential expression of numerous genes. Our current research focuses on characterizing novel SE-related genes in centaury tissues using next-generation RNA sequencing data and quantitative real-time PCR (qPCR). Six different centaury tissues, including embryogenic calli, globular and cotiledonary somatic embryos were subjected to ultra-high-throughput sequencing on an Illumina HiSeq 2500 platform, followed by de novo transcriptome assembly. FPKM (fragments per kilobase of exon model per million reads mapped) was used to evaluate in silico expression of functionally annotated transcripts. The generated transcriptome data were subsequently used for discovering genes with potential roles in centaury SE. Potential SE markers were excavated focusing on transcripts with greater than eight fold change in FPKM values in embryogenic tissues compared to non-embryogenic ones. Based on these criteria, twenty transcripts were chosen,
four with completely unknown function. Expression analysis of selected genes was carried out by qPCR using specific primers in different embryogenic and non-embryogenic centaury tissues. Identified genes with differential expression during SE will be further characterized by overexpression and silencing in centaury.
PB  - East Sarajevo: Faculty of Agriculture
C3  - Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina
T1  - Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis
SP  - 226
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5439
ER  - 

@conference{
author = "Ćuković, Katarina and Dragićević, Milan and Todorović, Slađana and Bogdanović, Milica and Simonović, Ana",
year = "2019",
abstract = "Centaurium erythraea Rafn. (Gentianaceae) represents a well-known medicinal plant rich in secondary metabolites and an appropriate model system for studying developmental biology due to its great developmental plasticity in vitro. One of the most unique manifestations of this characteristic in plants is somatic embryogenesis (SE), the development of embryos from somatic cells. SE initiation and progressing of the embryo through different developmental stages involves gene reprogramming and differential expression of numerous genes. Our current research focuses on characterizing novel SE-related genes in centaury tissues using next-generation RNA sequencing data and quantitative real-time PCR (qPCR). Six different centaury tissues, including embryogenic calli, globular and cotiledonary somatic embryos were subjected to ultra-high-throughput sequencing on an Illumina HiSeq 2500 platform, followed by de novo transcriptome assembly. FPKM (fragments per kilobase of exon model per million reads mapped) was used to evaluate in silico expression of functionally annotated transcripts. The generated transcriptome data were subsequently used for discovering genes with potential roles in centaury SE. Potential SE markers were excavated focusing on transcripts with greater than eight fold change in FPKM values in embryogenic tissues compared to non-embryogenic ones. Based on these criteria, twenty transcripts were chosen,
four with completely unknown function. Expression analysis of selected genes was carried out by qPCR using specific primers in different embryogenic and non-embryogenic centaury tissues. Identified genes with differential expression during SE will be further characterized by overexpression and silencing in centaury.",
publisher = "East Sarajevo: Faculty of Agriculture",
journal = "Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina",
title = "Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis",
pages = "226",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5439"
}

Ćuković, K., Dragićević, M., Todorović, S., Bogdanović, M.,& Simonović, A.. (2019). Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis. in Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina
East Sarajevo: Faculty of Agriculture., 226.
https://hdl.handle.net/21.15107/rcub_ibiss_5439

Ćuković K, Dragićević M, Todorović S, Bogdanović M, Simonović A. Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis. in Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina. 2019;:226.
https://hdl.handle.net/21.15107/rcub_ibiss_5439 .

Ćuković, Katarina, Dragićević, Milan, Todorović, Slađana, Bogdanović, Milica, Simonović, Ana, "Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis" in Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina (2019):226,
https://hdl.handle.net/21.15107/rcub_ibiss_5439 .

TY  - JOUR
AU  - Jevremović, Stojan
AU  - Lazarević, Jelica
AU  - Kostić, Miroslav
AU  - Krnjajić, Slobodan
AU  - Ugrenović, Vladan
AU  - Radonjić, Anda
AU  - Kostić, Igor
PY  - 2019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4391
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3657
AB  - The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, α-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and α-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.
T2  - Archives of Biological Sciences
T1  - Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans
IS  - 4
VL  - 71
DO  - 10.2298/ABS190617049J
SP  - 665
EP  - 676
ER  - 

@article{
author = "Jevremović, Stojan and Lazarević, Jelica and Kostić, Miroslav and Krnjajić, Slobodan and Ugrenović, Vladan and Radonjić, Anda and Kostić, Igor",
year = "2019",
abstract = "The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, α-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and α-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.",
journal = "Archives of Biological Sciences",
title = "Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans",
number = "4",
volume = "71",
doi = "10.2298/ABS190617049J",
pages = "665-676"
}

Jevremović, S., Lazarević, J., Kostić, M., Krnjajić, S., Ugrenović, V., Radonjić, A.,& Kostić, I.. (2019). Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences, 71(4), 665-676.
https://doi.org/10.2298/ABS190617049J

Jevremović S, Lazarević J, Kostić M, Krnjajić S, Ugrenović V, Radonjić A, Kostić I. Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences. 2019;71(4):665-676.
doi:10.2298/ABS190617049J .

Jevremović, Stojan, Lazarević, Jelica, Kostić, Miroslav, Krnjajić, Slobodan, Ugrenović, Vladan, Radonjić, Anda, Kostić, Igor, "Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans" in Archives of Biological Sciences, 71, no. 4 (2019):665-676,
https://doi.org/10.2298/ABS190617049J . .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Todorović, Slađana
AU  - Subotić, Angelina
AU  - Simonović, Ana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4335
AB  - Centaurium erythraea Rafn (Gentianaceae) is a medicinal plant, rich in secondary metabolites,
mainly secoiridoid glucosides and xanthones, known for numerous beneficial effects on human
health. In addition, C. erythraea possesses remarkable developmental plasticity and is easily cultured
in vitro, so it represents a suitable model system for studying developmental biology. One
of the most notable illustrations of plant cell totipotency is the capability of certain somatic plant
cells to initiate embryogenic development through somatic embryogenesis (SE). It has been proposed
that mechanical wounding during in vitro manipulations of plant material can promote cell
differentiation and somatic embryo development. Our recent research is based on elucidating
the gene expression profiles of centaury tissues in response to wounding and in different SE stages
using quantitative real-time PCR. Considering the fact that these processes exhibit a dynamic
genetic activity, the selection of stable reference genes is paramount in order to obtain unbiased
conclusions. Hereby we report selection of stable reference genes in C. erythraea for studying gene
expression during somatic embryogenesis and wounding. Thirteen frequently used reference
genes were selected and their expression stability was assessed in different developmental stages,
including globular and cotyledonary stages of embryos, as well as wounded tissues. Specific sets
of primers were designed relying on previously obtained next-generation RNA sequencing data.
The results were interpreted using two algorithmic approaches - geNorm and NormFinder - and
the most stable reference genes from a set of tested candidate genes were determined.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4335
ER  - 

@conference{
author = "Ćuković, Katarina and Paunović, Danijela and Bogdanović, Milica and Dragićević, Milan and Todorović, Slađana and Subotić, Angelina and Simonović, Ana",
year = "2018",
abstract = "Centaurium erythraea Rafn (Gentianaceae) is a medicinal plant, rich in secondary metabolites,
mainly secoiridoid glucosides and xanthones, known for numerous beneficial effects on human
health. In addition, C. erythraea possesses remarkable developmental plasticity and is easily cultured
in vitro, so it represents a suitable model system for studying developmental biology. One
of the most notable illustrations of plant cell totipotency is the capability of certain somatic plant
cells to initiate embryogenic development through somatic embryogenesis (SE). It has been proposed
that mechanical wounding during in vitro manipulations of plant material can promote cell
differentiation and somatic embryo development. Our recent research is based on elucidating
the gene expression profiles of centaury tissues in response to wounding and in different SE stages
using quantitative real-time PCR. Considering the fact that these processes exhibit a dynamic
genetic activity, the selection of stable reference genes is paramount in order to obtain unbiased
conclusions. Hereby we report selection of stable reference genes in C. erythraea for studying gene
expression during somatic embryogenesis and wounding. Thirteen frequently used reference
genes were selected and their expression stability was assessed in different developmental stages,
including globular and cotyledonary stages of embryos, as well as wounded tissues. Specific sets
of primers were designed relying on previously obtained next-generation RNA sequencing data.
The results were interpreted using two algorithmic approaches - geNorm and NormFinder - and
the most stable reference genes from a set of tested candidate genes were determined.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4335"
}

Ćuković, K., Paunović, D., Bogdanović, M., Dragićević, M., Todorović, S., Subotić, A.,& Simonović, A.. (2018). Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_4335

Ćuković K, Paunović D, Bogdanović M, Dragićević M, Todorović S, Subotić A, Simonović A. Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_4335 .

Ćuković, Katarina, Paunović, Danijela, Bogdanović, Milica, Dragićević, Milan, Todorović, Slađana, Subotić, Angelina, Simonović, Ana, "Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):28,
https://hdl.handle.net/21.15107/rcub_ibiss_4335 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Radojković, Aleksandar
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
AU  - Mitrović, Jelena
AU  - Kostić, Miroslav B.
AU  - Novaković, Tatjana
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0022474X17304083?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3051
AB  - For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (α-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8 < F/O-0.5 < F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.
T2  - Journal of Stored Products Research
T1  - Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)
VL  - 77
DO  - 10.1016/J.JSPR.2018.02.006
SP  - 45
EP  - 54
ER  - 

@article{
author = "Lazarević, Jelica and Radojković, Aleksandar and Kostić, Igor and Krnjajić, Slobodan and Mitrović, Jelena and Kostić, Miroslav B. and Novaković, Tatjana and Branković, Zorica and Branković, Goran",
year = "2018",
abstract = "For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (α-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8 < F/O-0.5 < F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.",
journal = "Journal of Stored Products Research",
title = "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)",
volume = "77",
doi = "10.1016/J.JSPR.2018.02.006",
pages = "45-54"
}

Lazarević, J., Radojković, A., Kostić, I., Krnjajić, S., Mitrović, J., Kostić, M. B., Novaković, T., Branković, Z.,& Branković, G.. (2018). Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research, 77, 45-54.
https://doi.org/10.1016/J.JSPR.2018.02.006

Lazarević J, Radojković A, Kostić I, Krnjajić S, Mitrović J, Kostić MB, Novaković T, Branković Z, Branković G. Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research. 2018;77:45-54.
doi:10.1016/J.JSPR.2018.02.006 .

Lazarević, Jelica, Radojković, Aleksandar, Kostić, Igor, Krnjajić, Slobodan, Mitrović, Jelena, Kostić, Miroslav B., Novaković, Tatjana, Branković, Zorica, Branković, Goran, "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)" in Journal of Stored Products Research, 77 (2018):45-54,
https://doi.org/10.1016/J.JSPR.2018.02.006 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Janković Tomanić, Milena
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Milanović, Slobodan
AU  - Stojković, Biljana
PY  - 2017
UR  - http://doi.wiley.com/10.1111/ens.12250
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2924
AB  - The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of α-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population × two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i.e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.
T2  - Entomological Science
T1  - Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)
IS  - 1
VL  - 20
DO  - 10.1111/ens.12250
SP  - 189
EP  - 194
ER  - 

@article{
author = "Lazarević, Jelica and Janković Tomanić, Milena and Savković, Uroš and Đorđević, Mirko and Milanović, Slobodan and Stojković, Biljana",
year = "2017",
abstract = "The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of α-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population × two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i.e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.",
journal = "Entomological Science",
title = "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)",
number = "1",
volume = "20",
doi = "10.1111/ens.12250",
pages = "189-194"
}

Lazarević, J., Janković Tomanić, M., Savković, U., Đorđević, M., Milanović, S.,& Stojković, B.. (2017). Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science, 20(1), 189-194.
https://doi.org/10.1111/ens.12250

Lazarević J, Janković Tomanić M, Savković U, Đorđević M, Milanović S, Stojković B. Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science. 2017;20(1):189-194.
doi:10.1111/ens.12250 .

Lazarević, Jelica, Janković Tomanić, Milena, Savković, Uroš, Đorđević, Mirko, Milanović, Slobodan, Stojković, Biljana, "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)" in Entomological Science, 20, no. 1 (2017):189-194,
https://doi.org/10.1111/ens.12250 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić-Komić, Sonja
AU  - Vuleta, Ana
AU  - Zechmann, Bernd
AU  - Prokić, Ljiljana
AU  - Veljović Jovanović, Sonja
AU  - Mock, H. P.
PY  - 2016
UR  - http://doi.wiley.com/10.1111/plb.12429
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2988
AB  - Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate–glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.
PB  - Blackwell Publishing Ltd
T2  - Plant Biology
T1  - Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants
IS  - 4
VL  - 18
DO  - 10.1111/plb.12429
SP  - 669
EP  - 680
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Milić-Komić, Sonja and Vuleta, Ana and Zechmann, Bernd and Prokić, Ljiljana and Veljović Jovanović, Sonja and Mock, H. P.",
year = "2016",
abstract = "Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate–glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.",
publisher = "Blackwell Publishing Ltd",
journal = "Plant Biology",
title = "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants",
number = "4",
volume = "18",
doi = "10.1111/plb.12429",
pages = "669-680"
}

Vidović, M., Morina, F., Milić-Komić, S., Vuleta, A., Zechmann, B., Prokić, L., Veljović Jovanović, S.,& Mock, H. P.. (2016). Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology
Blackwell Publishing Ltd., 18(4), 669-680.
https://doi.org/10.1111/plb.12429

Vidović M, Morina F, Milić-Komić S, Vuleta A, Zechmann B, Prokić L, Veljović Jovanović S, Mock HP. Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology. 2016;18(4):669-680.
doi:10.1111/plb.12429 .

Vidović, Marija, Morina, Filis, Milić-Komić, Sonja, Vuleta, Ana, Zechmann, Bernd, Prokić, Ljiljana, Veljović Jovanović, Sonja, Mock, H. P., "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants" in Plant Biology, 18, no. 4 (2016):669-680,
https://doi.org/10.1111/plb.12429 . .

TY  - JOUR
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Uskoković, Aleksandra
AU  - Stanković, Vesna
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Solujić, Slavica
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
PY  - 2014
UR  - http://www.ncbi.nlm.nih.gov/pubmed/24468630
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3185
AB  - Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.
PB  - Elsevier
T2  - European Journal of Pharmaceutical Sciences
T1  - Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.
IS  - 1
VL  - 55
DO  - 10.1016/j.ejps.2014.01.004
SP  - 20
EP  - 35
ER  - 

@article{
author = "Stanković, Nevena and Mladenović, Milan and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Uskoković, Aleksandra and Stanković, Vesna and Mihailović, Vladimir and Katanić, Jelena and Matić, Sanja and Solujić, Slavica and Vuković, Nenad and Sukdolak, Slobodan",
year = "2014",
abstract = "Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.",
publisher = "Elsevier",
journal = "European Journal of Pharmaceutical Sciences",
title = "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.",
number = "1",
volume = "55",
doi = "10.1016/j.ejps.2014.01.004",
pages = "20-35"
}

Stanković, N., Mladenović, M., Mihailović, M., Arambašić Jovanović, J., Uskoković, A., Stanković, V., Mihailović, V., Katanić, J., Matić, S., Solujić, S., Vuković, N.,& Sukdolak, S.. (2014). Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences
Elsevier., 55(1), 20-35.
https://doi.org/10.1016/j.ejps.2014.01.004

Stanković N, Mladenović M, Mihailović M, Arambašić Jovanović J, Uskoković A, Stanković V, Mihailović V, Katanić J, Matić S, Solujić S, Vuković N, Sukdolak S. Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences. 2014;55(1):20-35.
doi:10.1016/j.ejps.2014.01.004 .

Stanković, Nevena, Mladenović, Milan, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Uskoković, Aleksandra, Stanković, Vesna, Mihailović, Vladimir, Katanić, Jelena, Matić, Sanja, Solujić, Slavica, Vuković, Nenad, Sukdolak, Slobodan, "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model." in European Journal of Pharmaceutical Sciences, 55, no. 1 (2014):20-35,
https://doi.org/10.1016/j.ejps.2014.01.004 . .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://www.sciencedirect.com/science/article/pii/S1383571813001836
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3188
AB  - The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.
PB  - Elsevier
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury
IS  - 2
VL  - 755
DO  - 10.1016/j.mrgentox.2013.03.011
SP  - 81
EP  - 89
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Vidaković, Melita and Grdović, Nevena and Dinić, Svetlana and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Mirjana",
year = "2013",
abstract = "The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.",
publisher = "Elsevier",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury",
number = "2",
volume = "755",
doi = "10.1016/j.mrgentox.2013.03.011",
pages = "81-89"
}

Matić, S., Stanić, S., Bogojević, D., Vidaković, M., Grdović, N., Dinić, S., Solujić, S., Mladenović, M., Stanković, N.,& Mihailović, M.. (2013). Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier., 755(2), 81-89.
https://doi.org/10.1016/j.mrgentox.2013.03.011

Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Dinić S, Solujić S, Mladenović M, Stanković N, Mihailović M. Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2013;755(2):81-89.
doi:10.1016/j.mrgentox.2013.03.011 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Vidaković, Melita, Grdović, Nevena, Dinić, Svetlana, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Mirjana, "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 755, no. 2 (2013):81-89,
https://doi.org/10.1016/j.mrgentox.2013.03.011 . .