@article{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2019",
abstract = "Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequently
use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult
to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures
of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of
apple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain
15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used
as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate
the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,
kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected in
untransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry
(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production in
apple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicals
exuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantly
affected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days of
treatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsis
shoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanent
in vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.",
publisher = "Oxford:Oxford University Press",
journal = "Tree Physiology",
title = "Hairy root culture as a valuable tool for allelopathic studies in apple",
number = "5",
volume = "39",
doi = "doi:10.1093/treephys/tpz006",
pages = "888-905"
}