@article{
author = "Kirovski, Danijela and Dodovski, Petar and Savić, Đorđe and Vujanac, Ivan and Prodanović, Radiša and Mirilović, Milorad and Sladojević, Željko and Đorđević, Ana",
year = "2016",
abstract = "The aim of our study was to investigate if the thyroid axis of newborn calves is
affected by prenatal application of propylthyouracil (PTU). The study included 20
late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of
BW daily) from day 20 before expected calving until the day of calving. The other
group (n=10) was non-treated. Placental samples of dams were obtained for measuring
mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type
III (D3). After parturition calves were separated from the dams and included in the
study. Blood samples were taken daily from each calf starting on the day of birth until
day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of
the placental tissue revealed an abundance of all three types of placental deiodinases
in non-treated cows, and a signifi cant elevation of mRNA levels for all three types of
deiodinases after PTU treatment. Calves that originated from dams treated with PTU
had signifi cantly lower T3 and T4 and signifi cantly higher TSH concentrations compared
to non-treated calves during the fi rst 2 days of life. Starting from day 4 until day 6 of
life the opposite effect was observed meaning that calves prenatally exposed to PTU
had signifi cantly higher T3 and T4 and slightly lower TSH. Our study, for the fi rst time,
provides information related to iodothyronine deiodinases mRNA expression in bovine
placenta, and confi rm that PTU treatment of pregnant dams provokes depression of
thyroid function in newborns during the fi rst days of life., Cilj ovog rada bio je da se ispita da li je tireoidna osovina novorođene teladi
promenjena nakon prenatalne aplikacije propiltiouracila (PTU). U ogled je uključeno
20 krava holštajn rase u kasnom graviditetu. Prva grupa (n=10) je tretirana PTU (4
mg/kg telesne mase, dnevna doza) počevši od 20 dana pre očekivanog teljenja do dana
teljenja. Druga grupa (n=10) nije tretirana. Uzorci placente krava uzimani su u cilju
određivanja ekspresije iRNK dejodinaza tipa I (D1), tipa II (D2) i tipa III (D3). Nakon
teljenja, telad su odvojena od majki i uključena u ispitivanja. Uzorci krvi su uzimani
dnevno od svakog teleta počevši od dana rođenja do 7. dana života. U krvi su merene
koncentracije T3, T4 i TSH. PCR analiza tkiva placente je pokazala zastupljenost sva tri
tipa dejodinaza kod netretiranih krava, i značajno povećanje nivoa iRNK sva tri tipa
dejodinaza nakon tretmana PTU. Telad koja su poticala od majki tretiranih PTU imala
su značajno niže koncentracije T3 i T4 i značajno više koncentracije TSH u odnosu na
telad poreklom od netretiranih majki, tokom prva dva dana života. U periodu od 4.
do 6. dana života, utvrđen je suprotan efekat, što znači da su telad prenatalno izložena
delovanju PTU imala značajno više koncentracije T3 i T4 i značajno niže koncentracije
TSH u odnosu na netretiranu telad. Naša studija, po prvi put, daje informacije vezane
za ekspresiju dejodinaza u bovinoj placenti i potvrđuje da tretman gravidnih majki sa
PTU izaziva depresiju funkcije tireoidne osovine kod novorođenčadi tokom prva dva
dana neonatalnog života.",
publisher = "Belgrade: University of Belgrade- Faculty of Veterinary Medicine",
journal = "Acta Veterinaria, Beograd",
title = "Placental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (PTU) and thyroid axis activity of their calves, Ekspresija dejodinaza u placenti krava tretiranih sa propiltiouracilom (PTU) i aktivnost tireoidne osovine njihove teladi",
number = "1",
volume = "66",
doi = "10.1515/acve-2016-0005",
pages = "61-75"
}
