TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jevremović, Slađana
AU  - Petrović, Zoran Lj.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5415
AB  - In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins
PB  - Greifswald: Leibniz Institute for Plasma Science and Technology
C3  - Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
T1  - Changing the plant tissue morphology and physiology by plasma treatment
SP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5415
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Živković, Suzana and Milutinović, Milica and Jevremović, Slađana and Petrović, Zoran Lj.",
year = "2021",
abstract = "In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins",
publisher = "Greifswald: Leibniz Institute for Plasma Science and Technology",
journal = "Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany",
title = "Changing the plant tissue morphology and physiology by plasma treatment",
pages = "14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5415"
}

Puač, N., Škoro, N., Živković, S., Milutinović, M., Jevremović, S.,& Petrović, Z. Lj.. (2021). Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
Greifswald: Leibniz Institute for Plasma Science and Technology., 14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415

Puač N, Škoro N, Živković S, Milutinović M, Jevremović S, Petrović ZL. Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany. 2021;:14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

Puač, Nevena, Škoro, Nikola, Živković, Suzana, Milutinović, Milica, Jevremović, Slađana, Petrović, Zoran Lj., "Changing the plant tissue morphology and physiology by plasma treatment" in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany (2021):14,
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3756
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4966
AB  - Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
PB  - Oxford University Press
T2  - Glycobiology
T1  - ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R
IS  - 1
IS  - 1
VL  - 30
VL  - 30
DO  - 10.1093/glycob/cwz072
SP  - 19
SP  - 19
EP  - 35
EP  - 35
ER  - 

@article{
author = "Dragićević, Milan and Paunović, Danijela and Bogdanović, Milica and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.",
publisher = "Oxford University Press",
journal = "Glycobiology",
title = "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R",
number = "1, 1",
volume = "30, 30",
doi = "10.1093/glycob/cwz072",
pages = "19-19-35-35"
}

Dragićević, M., Paunović, D., Bogdanović, M., Todorović, S.,& Simonović, A.. (2020). ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology
Oxford University Press., 30(1), 19-35.
https://doi.org/10.1093/glycob/cwz072

Dragićević M, Paunović D, Bogdanović M, Todorović S, Simonović A. ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology. 2020;30(1):19-35.
doi:10.1093/glycob/cwz072 .

Dragićević, Milan, Paunović, Danijela, Bogdanović, Milica, Todorović, Slađana, Simonović, Ana, "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R" in Glycobiology, 30, no. 1 (2020):19-35,
https://doi.org/10.1093/glycob/cwz072 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3756
AB  - Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
PB  - Oxford University Press
T2  - Glycobiology
T1  - ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R
IS  - 1
IS  - 1
VL  - 30
VL  - 30
DO  - 10.1093/glycob/cwz072
SP  - 19
SP  - 19
EP  - 35
EP  - 35
ER  - 

@article{
author = "Dragićević, Milan and Paunović, Danijela and Bogdanović, Milica and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.",
publisher = "Oxford University Press",
journal = "Glycobiology",
title = "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R",
number = "1, 1",
volume = "30, 30",
doi = "10.1093/glycob/cwz072",
pages = "19-19-35-35"
}

Dragićević, M., Paunović, D., Bogdanović, M., Todorović, S.,& Simonović, A.. (2020). ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology
Oxford University Press., 30(1), 19-35.
https://doi.org/10.1093/glycob/cwz072

Dragićević M, Paunović D, Bogdanović M, Todorović S, Simonović A. ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology. 2020;30(1):19-35.
doi:10.1093/glycob/cwz072 .

Dragićević, Milan, Paunović, Danijela, Bogdanović, Milica, Todorović, Slađana, Simonović, Ana, "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R" in Glycobiology, 30, no. 1 (2020):19-35,
https://doi.org/10.1093/glycob/cwz072 . .

TY  - THES
AU  - Trajković, Milena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3684
AB  - Razvijen je protokol za regeneraciju biljaka Viola cornuta L. cv. ’Lutea Splendens’, sitnocvetne ljubičice sa cvetovima žute boje, i genetičku transformaciju genom za kapsantin-kapsorubin-sintazu u cilju promene boje cveta metaboličkom modifikacijom biosinteze karotenoida. Najuspešnija indukcija adventivnih izdanaka dobijena je od eksplantata hipokotila gajenih na ½MS hranljivoj podlozi sa 0,1 mg/l 2,4-D i 2 mg/l BAP u uslovima dugog dana. Indukcija adventivnih izdanaka odvijala se procesom indirektne i direktne organogeneze. Transformisani izdanci dobijeni su genetičkom transformacijom pomoću ”praznog” (Llccs-) i pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vektora, dok je korišćenjem pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vektora dobijen transformisani kalus. Prisustvo Llccs, hpt i uidA gena u genomu V. cornuta potvrđeno je PCR analizom, dok je qPCR analiza pokazala neujednačen nivo ekspresije Llccs gena u kruničnim listićima cvetova i listovima 35S-Llccs transformisanih linija. Boja kruničnih listića cvetova i žiga tučka promenjena je iz žute, odnosno zelene u nijanse narandžaste boje, usled akumulacije kapsantina, novosintetisanog crvenog pigmenta u hromoplastima 35S-Llccs transformisanih biljaka. Fenotip transformisanih biljaka se značajno razlikovao od fenotipa netransformisanih biljaka. Razvijen je protokol za dugotrajno čuvanje (krioprezervaciju) netransformisanih i transformisanih linija korišćenjem metode vitrifikacije sa PVS3 rastvorom. Oporavak vrhova izdanaka i regeneracija biljaka transformisanih linija (Llccs- i 35S-Llccs) posle krioprezervacije bili su značajno manji u odnosu na oporavak vrhova netransformisanih izdanaka. Nivo ploidnosti biljaka V. cornuta regenerisanih u kulturi in vitro, transformisanih linija kao i biljaka regenerisanih posle krioprezervacije bio je identičan.
AB  - A protocol for the plant regeneration of Viola cornuta L. cv. ’Lutea Splendens’, horned pansy with yellow flowers and the genetic transformation with the capsanthin-capsorubin-synthase gene in order to change flower color by metabolic modification of carotenoid biosynthesis were developed. The most efficient induction of adventitious shoots was obtained from hypocotyl explants grown on ½MS medium with 0,1 mg/l 2,4-D and 2 mg/l BAP under 16h light/8h dark conditions. The induction of adventitious shoots was achieved through a process of indirect and direct organogenesis.Transformed shoots were obtained by genetic transformation using ”empty” (Llccs-) and pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vectors, while using pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vectors transformed calli were obtained. The presence of the Llccs, hpt and uidA genes in the V. cornuta transformed plants were confirmed by PCR analysis, while qPCR analysis showed an unequal level of Llccs gene expression in the petals and leaves of 35S-Llccs transformed lines. The color of the flower petals and the stigma changed from yellow or green to shades of orange, due to the accumulation of capsanthin, a newly synthesized red pigment in the chromoplasts of 35S-Llccs transformed plants. The phenotype of transformed plants differed significantly from the phenotype of untransformed plants. A protocol for the long-term storage (cryopreservation) of untransformed and transformed lines using the vitrification method with PVS3 solution has been developed. The recovery of shoot tips and regeneration of transformed lines (Llccs- and 35S-Llccs) after cryopreservation was significantly lower compared to the recovery of the shoot tips of the untransformed shoots. The ploidy levels of V. cornuta plants regenerated in culture in vitro, transformed lines and plants recovered after cryopreservation were similar.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu
T1  - Plant regeneration in vitro and genetic transformation of Viola cornuta (Violaceae) using capsanthin-capsorubin-synthase gene
SP  - 1
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3684
ER  - 

@phdthesis{
author = "Trajković, Milena",
year = "2020",
abstract = "Razvijen je protokol za regeneraciju biljaka Viola cornuta L. cv. ’Lutea Splendens’, sitnocvetne ljubičice sa cvetovima žute boje, i genetičku transformaciju genom za kapsantin-kapsorubin-sintazu u cilju promene boje cveta metaboličkom modifikacijom biosinteze karotenoida. Najuspešnija indukcija adventivnih izdanaka dobijena je od eksplantata hipokotila gajenih na ½MS hranljivoj podlozi sa 0,1 mg/l 2,4-D i 2 mg/l BAP u uslovima dugog dana. Indukcija adventivnih izdanaka odvijala se procesom indirektne i direktne organogeneze. Transformisani izdanci dobijeni su genetičkom transformacijom pomoću ”praznog” (Llccs-) i pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vektora, dok je korišćenjem pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vektora dobijen transformisani kalus. Prisustvo Llccs, hpt i uidA gena u genomu V. cornuta potvrđeno je PCR analizom, dok je qPCR analiza pokazala neujednačen nivo ekspresije Llccs gena u kruničnim listićima cvetova i listovima 35S-Llccs transformisanih linija. Boja kruničnih listića cvetova i žiga tučka promenjena je iz žute, odnosno zelene u nijanse narandžaste boje, usled akumulacije kapsantina, novosintetisanog crvenog pigmenta u hromoplastima 35S-Llccs transformisanih biljaka. Fenotip transformisanih biljaka se značajno razlikovao od fenotipa netransformisanih biljaka. Razvijen je protokol za dugotrajno čuvanje (krioprezervaciju) netransformisanih i transformisanih linija korišćenjem metode vitrifikacije sa PVS3 rastvorom. Oporavak vrhova izdanaka i regeneracija biljaka transformisanih linija (Llccs- i 35S-Llccs) posle krioprezervacije bili su značajno manji u odnosu na oporavak vrhova netransformisanih izdanaka. Nivo ploidnosti biljaka V. cornuta regenerisanih u kulturi in vitro, transformisanih linija kao i biljaka regenerisanih posle krioprezervacije bio je identičan., A protocol for the plant regeneration of Viola cornuta L. cv. ’Lutea Splendens’, horned pansy with yellow flowers and the genetic transformation with the capsanthin-capsorubin-synthase gene in order to change flower color by metabolic modification of carotenoid biosynthesis were developed. The most efficient induction of adventitious shoots was obtained from hypocotyl explants grown on ½MS medium with 0,1 mg/l 2,4-D and 2 mg/l BAP under 16h light/8h dark conditions. The induction of adventitious shoots was achieved through a process of indirect and direct organogenesis.Transformed shoots were obtained by genetic transformation using ”empty” (Llccs-) and pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vectors, while using pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vectors transformed calli were obtained. The presence of the Llccs, hpt and uidA genes in the V. cornuta transformed plants were confirmed by PCR analysis, while qPCR analysis showed an unequal level of Llccs gene expression in the petals and leaves of 35S-Llccs transformed lines. The color of the flower petals and the stigma changed from yellow or green to shades of orange, due to the accumulation of capsanthin, a newly synthesized red pigment in the chromoplasts of 35S-Llccs transformed plants. The phenotype of transformed plants differed significantly from the phenotype of untransformed plants. A protocol for the long-term storage (cryopreservation) of untransformed and transformed lines using the vitrification method with PVS3 solution has been developed. The recovery of shoot tips and regeneration of transformed lines (Llccs- and 35S-Llccs) after cryopreservation was significantly lower compared to the recovery of the shoot tips of the untransformed shoots. The ploidy levels of V. cornuta plants regenerated in culture in vitro, transformed lines and plants recovered after cryopreservation were similar.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu, Plant regeneration in vitro and genetic transformation of Viola cornuta (Violaceae) using capsanthin-capsorubin-synthase gene",
pages = "1-143",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3684"
}

Trajković, M.. (2020). Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-143.
https://hdl.handle.net/21.15107/rcub_ibiss_3684

Trajković M. Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu. in University of Belgrade, Faculty of Biology. 2020;:1-143.
https://hdl.handle.net/21.15107/rcub_ibiss_3684 .

Trajković, Milena, "Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu" in University of Belgrade, Faculty of Biology (2020):1-143,
https://hdl.handle.net/21.15107/rcub_ibiss_3684 .

TY  - JOUR
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Paunović, Danijela
AU  - Giurato, Giorgio
AU  - Filipović, Biljana
AU  - Subotić, Angelina
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3686
AB  - Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis
IS  - 2
VL  - 141
DO  - 10.1007/s11240-020-01801-w
SP  - 417
EP  - 433
ER  - 

@article{
author = "Ćuković, Katarina and Dragićević, Milan and Bogdanović, Milica and Paunović, Danijela and Giurato, Giorgio and Filipović, Biljana and Subotić, Angelina and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis",
number = "2",
volume = "141",
doi = "10.1007/s11240-020-01801-w",
pages = "417-433"
}

Ćuković, K., Dragićević, M., Bogdanović, M., Paunović, D., Giurato, G., Filipović, B., Subotić, A., Todorović, S.,& Simonović, A.. (2020). Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture
Springer., 141(2), 417-433.
https://doi.org/10.1007/s11240-020-01801-w

Ćuković K, Dragićević M, Bogdanović M, Paunović D, Giurato G, Filipović B, Subotić A, Todorović S, Simonović A. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture. 2020;141(2):417-433.
doi:10.1007/s11240-020-01801-w .

Ćuković, Katarina, Dragićević, Milan, Bogdanović, Milica, Paunović, Danijela, Giurato, Giorgio, Filipović, Biljana, Subotić, Angelina, Todorović, Slađana, Simonović, Ana, "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis" in Plant Cell, Tissue and Organ Culture, 141, no. 2 (2020):417-433,
https://doi.org/10.1007/s11240-020-01801-w . .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Petrović, Zoran Lj
AU  - Škoro, Nikola
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4398
AB  - The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.
PB  - Heron Press
C3  - Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
T1  - Alteration in plant tissue morphology and physiology induced by plasma treatment
SP  - 63
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4398
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Milutinović, Milica and Puač, Nevena and Petrović, Zoran Lj and Škoro, Nikola",
year = "2019",
abstract = "The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.",
publisher = "Heron Press",
journal = "Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)",
title = "Alteration in plant tissue morphology and physiology induced by plasma treatment",
pages = "63-64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4398"
}

Živković, S., Jevremović, S., Milutinović, M., Puač, N., Petrović, Z. L.,& Škoro, N.. (2019). Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
Heron Press., 63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398

Živković S, Jevremović S, Milutinović M, Puač N, Petrović ZL, Škoro N. Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019). 2019;:63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

Živković, Suzana, Jevremović, Slađana, Milutinović, Milica, Puač, Nevena, Petrović, Zoran Lj, Škoro, Nikola, "Alteration in plant tissue morphology and physiology induced by plasma treatment" in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019) (2019):63-64,
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

TY  - JOUR
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Ghalawenji, Nabil
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2019
UR  - http://link.springer.com/10.1007/s13205-018-1540-4
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6314937
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3232
AB  - The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.
T2  - 3 Biotech
T1  - Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.
IS  - 1
VL  - 9
DO  - 10.1007/s13205-018-1540-4
SP  - 17
ER  - 

@article{
author = "Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Ghalawenji, Nabil and Subotić, Angelina and Jevremović, Slađana",
year = "2019",
abstract = "The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.",
journal = "3 Biotech",
title = "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.",
number = "1",
volume = "9",
doi = "10.1007/s13205-018-1540-4",
pages = "17"
}

Trajković, M., Antonić Reljin, D., Cingel, A., Ghalawenji, N., Subotić, A.,& Jevremović, S.. (2019). Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech, 9(1), 17.
https://doi.org/10.1007/s13205-018-1540-4

Trajković M, Antonić Reljin D, Cingel A, Ghalawenji N, Subotić A, Jevremović S. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech. 2019;9(1):17.
doi:10.1007/s13205-018-1540-4 .

Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Ghalawenji, Nabil, Subotić, Angelina, Jevremović, Slađana, "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta." in 3 Biotech, 9, no. 1 (2019):17,
https://doi.org/10.1007/s13205-018-1540-4 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Trailović, Maja
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6722
AB  - Cryopreservation represents a suitable method for long term storage of different plant genetic
resources. The aim of this study was to develop protocol for cryopreservation of Viola cornuta
shoot tips using one step freezing method with chemical dehydration of tissue with modified
Plant Vitrification Solutions (PVS2 or PVS3). Shoot tips (1-2 mm) of two-week cold acclimated
shoots were cultured on ½MS medium with 0.3 M sucrose for one day before treatment with loading
solution (2 M glycerol, 0.4 M sucrose) for 30 min. Osmotic dehydration with PVS2 solution (30% glycerol, 15% ethylene glycol and 15% DMSO in liquid ½MS medium with 0.4 M sucrose) were
tested at 0 °C or 24 °C. Osmotic dehydration with PVS3 (50% sucrose, 50% glycerol in liquid ½MS
medium) were tested at 24 °C for 45 min. After the treatment the explants were directly immersed
in liquid nitrogen (LN) for at least one day. Re-warming was performed at 42 °C in water bath
for 2 min. After re-warming, the PVS solutions were replaced with unloading solution containing
1.2 M sucrose for 20 min. Re-warmed shoot tips were cultured on ½MS medium with 0.1 mg L-1
BAP. We observed that PVS2 solution is cytotoxic for V. cornuta shoot tips and cannot be used for
cryopreservation. However, cryopreservation with PVS3 solution was successful, where 71.9-100%
shoot tips survived treatment before immersion to LN and 31-40% survived after re-warming from
LN. Regrowth of cryopreserved shoot tips with new well-formed leaves was obtained after four
weeks of culture.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Cryopreservation of Viola cornuta shoot tips using vitrification procedure
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6722
ER  - 

@conference{
author = "Trajković, Milena and Antonić Reljin, Dragana and Trailović, Maja and Trifunović-Momčilov, Milana and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Cryopreservation represents a suitable method for long term storage of different plant genetic
resources. The aim of this study was to develop protocol for cryopreservation of Viola cornuta
shoot tips using one step freezing method with chemical dehydration of tissue with modified
Plant Vitrification Solutions (PVS2 or PVS3). Shoot tips (1-2 mm) of two-week cold acclimated
shoots were cultured on ½MS medium with 0.3 M sucrose for one day before treatment with loading
solution (2 M glycerol, 0.4 M sucrose) for 30 min. Osmotic dehydration with PVS2 solution (30% glycerol, 15% ethylene glycol and 15% DMSO in liquid ½MS medium with 0.4 M sucrose) were
tested at 0 °C or 24 °C. Osmotic dehydration with PVS3 (50% sucrose, 50% glycerol in liquid ½MS
medium) were tested at 24 °C for 45 min. After the treatment the explants were directly immersed
in liquid nitrogen (LN) for at least one day. Re-warming was performed at 42 °C in water bath
for 2 min. After re-warming, the PVS solutions were replaced with unloading solution containing
1.2 M sucrose for 20 min. Re-warmed shoot tips were cultured on ½MS medium with 0.1 mg L-1
BAP. We observed that PVS2 solution is cytotoxic for V. cornuta shoot tips and cannot be used for
cryopreservation. However, cryopreservation with PVS3 solution was successful, where 71.9-100%
shoot tips survived treatment before immersion to LN and 31-40% survived after re-warming from
LN. Regrowth of cryopreserved shoot tips with new well-formed leaves was obtained after four
weeks of culture.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Cryopreservation of Viola cornuta shoot tips using vitrification procedure",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6722"
}

Trajković, M., Antonić Reljin, D., Trailović, M., Trifunović-Momčilov, M., Subotić, A.,& Jevremović, S.. (2018). Cryopreservation of Viola cornuta shoot tips using vitrification procedure. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6722

Trajković M, Antonić Reljin D, Trailović M, Trifunović-Momčilov M, Subotić A, Jevremović S. Cryopreservation of Viola cornuta shoot tips using vitrification procedure. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6722 .

Trajković, Milena, Antonić Reljin, Dragana, Trailović, Maja, Trifunović-Momčilov, Milana, Subotić, Angelina, Jevremović, Slađana, "Cryopreservation of Viola cornuta shoot tips using vitrification procedure" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6722 .

TY  - CONF
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6540
AB  - Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6540
ER  - 

@conference{
author = "Trajković, Milena and Jeknić, Zoran and Antonić Reljin, Dragana and Subotić, Angelina and Jevremović, Slađana and Cingel, Aleksandar",
year = "2018",
abstract = "Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene",
pages = "152",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6540"
}

Trajković, M., Jeknić, Z., Antonić Reljin, D., Subotić, A., Jevremović, S.,& Cingel, A.. (2018). Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade., 152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540

Trajković M, Jeknić Z, Antonić Reljin D, Subotić A, Jevremović S, Cingel A. Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

Trajković, Milena, Jeknić, Zoran, Antonić Reljin, Dragana, Subotić, Angelina, Jevremović, Slađana, Cingel, Aleksandar, "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):152,
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Trailović, Maja
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6394
AB  - Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).
PB  - Belgrade: Serbian Biological Socitey
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6394
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Trailović, Maja and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).",
publisher = "Belgrade: Serbian Biological Socitey",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6394"
}

Antonić Reljin, D., Trailović, M., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2018). Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Socitey., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394

Antonić Reljin D, Trailović M, Trajković M, Cingel A, Subotić A, Jevremović S. Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

Antonić Reljin, Dragana, Trailović, Maja, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):60,
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

TY  - CONF
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Simonović, Ana
AU  - Dragićević, Milan
PY  - 2018
UR  - https://ojs.pmf.uns.ac.rs/index.php/dbe_serbica/issue/view/25
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4341
AB  - Plant Hydroxyproline rich glycoproteins (HGRPs) comprise a highly diverse family of cell wall macromolecules, involved in a wide array of physiological functions such as cell expansion, somatic embryogenesis, self-incompatibility, signaling and pathogen responses. Due to biased amino acid composition, abundant in disorder promoting residues, HRGPs are intrinsically disordered proteins. The lack of a stable structure lessens the sequence constraints imposed on these proteins and hampers efforts for homology based identification. Current mining approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.

Herby we present ragp, an R package for HGRP mining with a pipeline which emphasizes finding chimeric and short HRGP’s which is especially useful for identification of arabinogalactan proteins. The ragp pipeline exploits one of HGRP key features, the presence of hydroxyprolines which represent glycosylation sites. These sites are identified using a gradient boosting model trained on plant sequences with experimentally determined hydroxyprolines, based on the local (21-mer) sequence around the target prolines. The model was validated on a set of sequences which were not used during the model building, as well as by using several resampling approaches. Apart from prediction of proline hydroxylation main ragp features include efficient communication with web servers for prediction of N-terminal signal peptides and GPI modification sites, sequence annotation by querying hmmscan, GO enrichment based on predicted pfam domains, and the ability to classify prototypical HRGPs. 

ragp represents the first implementation of a HRGP mining workflow in the R statistical language. It implements common strategies for finding and classifying HRGP sequences along with an optional step where proline hydroxylation is estimated which leads to increased sensitivity and specificity of the filtered sequences. Since R is one of the leading bioinformatics platforms, the filtered sequences can be further analyzed by many specialized packages using the same environment.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Biologia Serbica: BELBI2018, 18. - 22 June 2018., Belgrade, Serbia, Book of Abstracts, p89
T1  - ragp: An R toolbox for mining Hydroxyproline rich glycoproteins
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4341
ER  - 

@conference{
author = "Paunović, Danijela and Bogdanović, Milica and Todorović, Slađana and Simonović, Ana and Dragićević, Milan",
year = "2018",
abstract = "Plant Hydroxyproline rich glycoproteins (HGRPs) comprise a highly diverse family of cell wall macromolecules, involved in a wide array of physiological functions such as cell expansion, somatic embryogenesis, self-incompatibility, signaling and pathogen responses. Due to biased amino acid composition, abundant in disorder promoting residues, HRGPs are intrinsically disordered proteins. The lack of a stable structure lessens the sequence constraints imposed on these proteins and hampers efforts for homology based identification. Current mining approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences.

Herby we present ragp, an R package for HGRP mining with a pipeline which emphasizes finding chimeric and short HRGP’s which is especially useful for identification of arabinogalactan proteins. The ragp pipeline exploits one of HGRP key features, the presence of hydroxyprolines which represent glycosylation sites. These sites are identified using a gradient boosting model trained on plant sequences with experimentally determined hydroxyprolines, based on the local (21-mer) sequence around the target prolines. The model was validated on a set of sequences which were not used during the model building, as well as by using several resampling approaches. Apart from prediction of proline hydroxylation main ragp features include efficient communication with web servers for prediction of N-terminal signal peptides and GPI modification sites, sequence annotation by querying hmmscan, GO enrichment based on predicted pfam domains, and the ability to classify prototypical HRGPs. 

ragp represents the first implementation of a HRGP mining workflow in the R statistical language. It implements common strategies for finding and classifying HRGP sequences along with an optional step where proline hydroxylation is estimated which leads to increased sensitivity and specificity of the filtered sequences. Since R is one of the leading bioinformatics platforms, the filtered sequences can be further analyzed by many specialized packages using the same environment.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Biologia Serbica: BELBI2018, 18. - 22 June 2018., Belgrade, Serbia, Book of Abstracts, p89",
title = "ragp: An R toolbox for mining Hydroxyproline rich glycoproteins",
pages = "89",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4341"
}

Paunović, D., Bogdanović, M., Todorović, S., Simonović, A.,& Dragićević, M.. (2018). ragp: An R toolbox for mining Hydroxyproline rich glycoproteins. in Biologia Serbica: BELBI2018, 18. - 22 June 2018., Belgrade, Serbia, Book of Abstracts, p89
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 89.
https://hdl.handle.net/21.15107/rcub_ibiss_4341

Paunović D, Bogdanović M, Todorović S, Simonović A, Dragićević M. ragp: An R toolbox for mining Hydroxyproline rich glycoproteins. in Biologia Serbica: BELBI2018, 18. - 22 June 2018., Belgrade, Serbia, Book of Abstracts, p89. 2018;:89.
https://hdl.handle.net/21.15107/rcub_ibiss_4341 .

Paunović, Danijela, Bogdanović, Milica, Todorović, Slađana, Simonović, Ana, Dragićević, Milan, "ragp: An R toolbox for mining Hydroxyproline rich glycoproteins" in Biologia Serbica: BELBI2018, 18. - 22 June 2018., Belgrade, Serbia, Book of Abstracts, p89 (2018):89,
https://hdl.handle.net/21.15107/rcub_ibiss_4341 .

TY  - CONF
AU  - Simonović, Ana
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Ćuković, Katarina
AU  - Subotić, Angelina
AU  - Paunović, Danijela
AU  - Todorović, Slađana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4339
AB  - Kičica je lekovita biljka bogata sekoiridoidima i ksantonima. Potreba za in vitro razmnožavanjem kičice je dovela do razvoja protokola za mikropropagaciju, organogenezu, somatsku embriogenezu (SE), kulturu ćelija, kalusa i korenova i genetičke transformacije.1 Kičica ima izvanredan regeneracioni potencijal i morfogenetsku plastičnost in vitro.1 Polazeći od pretpostavke da se geni uključeni u morfogenezu kod ovakve biljke lako aktiviraju pod induktivnim in vitro uslovima, te da se mogu detektovati kao transkripti potencijalnih markera SE i organogeneze, u cilju identifikacije tih gena smo uspostavili eksperimantalno-analitički sistem koji obuhvata: 1) protokole organogeneze, SE i transformacije; 2) foto-informacioni sistem za "time-laps" dokumentaciju razvoja biljaka in vitro; 3) sekvencirane i rekonstruisane transkriptome lista, korena, embriogenog kalusa, globularnih i kotiledonarnih somatskih embriona i adventivnih pupoljaka kičice; 4) bioinformatičke metode anotacije i analize transkriptoma; 5) identifikovane diferencijalno eksprimirane gene; 6) kolekciju od 17 tkiva/RNK uzoraka za qPCR analizu; 7) set referentnih gena konstantne ekspresije, kao i 8) sofisticiran bioinformatički metod u R-u za identifikaciju proteina sa hidroksiprolinom. Ovaj metod ima primenu u pretrazi biljnih proteina bogatih hidroksiprolinom, a kod kičice će biti korišćen za identifikaciju arabinogalaktanskih proteina, za koje smo pokazali da se indukuju tokom morfogeneze.2,3
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije, Kladovo
T1  - Identifikacija gena koji učestvuju u morfogenezi in vitro kod kičice (Centaurium erythraea Rafn.)
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4339
ER  - 

@conference{
author = "Simonović, Ana and Bogdanović, Milica and Dragićević, Milan and Ćuković, Katarina and Subotić, Angelina and Paunović, Danijela and Todorović, Slađana",
year = "2018",
abstract = "Kičica je lekovita biljka bogata sekoiridoidima i ksantonima. Potreba za in vitro razmnožavanjem kičice je dovela do razvoja protokola za mikropropagaciju, organogenezu, somatsku embriogenezu (SE), kulturu ćelija, kalusa i korenova i genetičke transformacije.1 Kičica ima izvanredan regeneracioni potencijal i morfogenetsku plastičnost in vitro.1 Polazeći od pretpostavke da se geni uključeni u morfogenezu kod ovakve biljke lako aktiviraju pod induktivnim in vitro uslovima, te da se mogu detektovati kao transkripti potencijalnih markera SE i organogeneze, u cilju identifikacije tih gena smo uspostavili eksperimantalno-analitički sistem koji obuhvata: 1) protokole organogeneze, SE i transformacije; 2) foto-informacioni sistem za "time-laps" dokumentaciju razvoja biljaka in vitro; 3) sekvencirane i rekonstruisane transkriptome lista, korena, embriogenog kalusa, globularnih i kotiledonarnih somatskih embriona i adventivnih pupoljaka kičice; 4) bioinformatičke metode anotacije i analize transkriptoma; 5) identifikovane diferencijalno eksprimirane gene; 6) kolekciju od 17 tkiva/RNK uzoraka za qPCR analizu; 7) set referentnih gena konstantne ekspresije, kao i 8) sofisticiran bioinformatički metod u R-u za identifikaciju proteina sa hidroksiprolinom. Ovaj metod ima primenu u pretrazi biljnih proteina bogatih hidroksiprolinom, a kod kičice će biti korišćen za identifikaciju arabinogalaktanskih proteina, za koje smo pokazali da se indukuju tokom morfogeneze.2,3",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije, Kladovo",
title = "Identifikacija gena koji učestvuju u morfogenezi in vitro kod kičice (Centaurium erythraea Rafn.)",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4339"
}

Simonović, A., Bogdanović, M., Dragićević, M., Ćuković, K., Subotić, A., Paunović, D.,& Todorović, S.. (2018). Identifikacija gena koji učestvuju u morfogenezi in vitro kod kičice (Centaurium erythraea Rafn.). in Drugi kongres biologa Srbije, Kladovo
Belgrade: Serbian Biological Society., 44.
https://hdl.handle.net/21.15107/rcub_ibiss_4339

Simonović A, Bogdanović M, Dragićević M, Ćuković K, Subotić A, Paunović D, Todorović S. Identifikacija gena koji učestvuju u morfogenezi in vitro kod kičice (Centaurium erythraea Rafn.). in Drugi kongres biologa Srbije, Kladovo. 2018;:44.
https://hdl.handle.net/21.15107/rcub_ibiss_4339 .

Simonović, Ana, Bogdanović, Milica, Dragićević, Milan, Ćuković, Katarina, Subotić, Angelina, Paunović, Danijela, Todorović, Slađana, "Identifikacija gena koji učestvuju u morfogenezi in vitro kod kičice (Centaurium erythraea Rafn.)" in Drugi kongres biologa Srbije, Kladovo (2018):44,
https://hdl.handle.net/21.15107/rcub_ibiss_4339 .

TY  - CONF
AU  - Filipović, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4387
AB  - Arabinogalactan proteins (AGPs) are a family of ubiquitous hydroxyproline-rich glycoproteins present in plasma membranes, cell walls and secretions of plants. Since AGPs are highly glycosylated, more than 90% of their total molecular mass comes from carbohydrate moieties consisting of various arabinogalactosyl chains (AG sugar chains), which are thought to be important for thediverse functions of AGPs. AGPs are implicated in many aspects of plant growth and development, including cell differentiation, organogenesis and somatic embryogenesis (SE). The localization of AG sugar chains in plant tissues can be visualised using monoclonal antibodies (mAbs) that can detect different AGP epitopes. The aim of this study was to investigate changes in the localization of AGPs during induction of indirect SE and shoot organogenesis on leaf explants of centaury. Immunofluorescence labelling of leaf sections was performed with a set of mAbs (MAC207, JIM4,  JIM8, JIM13, JIM15, LM2 and LM14). The results revealed that AGPs recognized by all mAbs tested were expressed in numerous meristematic cells from which somatic embryos develop. The AGP epitope recognised by the JIM4 antibody showed stronger intensity of immunofluorescence in the cell walls of protodermal cells of globular somatic embryos, whilst MAC207 and JIM13 epitopes were detected in cotyledonary somatic embryos. Strong immunofluorescence of the AGPs epitopes recognized with JIM4 and MAC207 mAbs were observed in the cells of apical meristem and leaf primordia of adventitious buds. These results suggest that AGPs play an important developmental role during formation of somatic embryos and adventitious buds from leaf explants of centaury.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade
T1  - Distribution of some arabinogalactan protein epitopes during somatic embryogenesis and organogenesis on leaf explants on centaury (Centaurium erythraea Rafn)
SP  - 21
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4387
ER  - 

@conference{
author = "Filipović, Biljana and Trifunović-Momčilov, Milana and Simonović, Ana and Jevremović, Slađana and Subotić, Angelina",
year = "2018",
abstract = "Arabinogalactan proteins (AGPs) are a family of ubiquitous hydroxyproline-rich glycoproteins present in plasma membranes, cell walls and secretions of plants. Since AGPs are highly glycosylated, more than 90% of their total molecular mass comes from carbohydrate moieties consisting of various arabinogalactosyl chains (AG sugar chains), which are thought to be important for thediverse functions of AGPs. AGPs are implicated in many aspects of plant growth and development, including cell differentiation, organogenesis and somatic embryogenesis (SE). The localization of AG sugar chains in plant tissues can be visualised using monoclonal antibodies (mAbs) that can detect different AGP epitopes. The aim of this study was to investigate changes in the localization of AGPs during induction of indirect SE and shoot organogenesis on leaf explants of centaury. Immunofluorescence labelling of leaf sections was performed with a set of mAbs (MAC207, JIM4,  JIM8, JIM13, JIM15, LM2 and LM14). The results revealed that AGPs recognized by all mAbs tested were expressed in numerous meristematic cells from which somatic embryos develop. The AGP epitope recognised by the JIM4 antibody showed stronger intensity of immunofluorescence in the cell walls of protodermal cells of globular somatic embryos, whilst MAC207 and JIM13 epitopes were detected in cotyledonary somatic embryos. Strong immunofluorescence of the AGPs epitopes recognized with JIM4 and MAC207 mAbs were observed in the cells of apical meristem and leaf primordia of adventitious buds. These results suggest that AGPs play an important developmental role during formation of somatic embryos and adventitious buds from leaf explants of centaury.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade",
title = "Distribution of some arabinogalactan protein epitopes during somatic embryogenesis and organogenesis on leaf explants on centaury (Centaurium erythraea Rafn)",
pages = "21-22",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4387"
}

Filipović, B., Trifunović-Momčilov, M., Simonović, A., Jevremović, S.,& Subotić, A.. (2018). Distribution of some arabinogalactan protein epitopes during somatic embryogenesis and organogenesis on leaf explants on centaury (Centaurium erythraea Rafn). in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade
Belgrade: Serbian Plant Physiology Society., 21-22.
https://hdl.handle.net/21.15107/rcub_ibiss_4387

Filipović B, Trifunović-Momčilov M, Simonović A, Jevremović S, Subotić A. Distribution of some arabinogalactan protein epitopes during somatic embryogenesis and organogenesis on leaf explants on centaury (Centaurium erythraea Rafn). in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade. 2018;:21-22.
https://hdl.handle.net/21.15107/rcub_ibiss_4387 .

Filipović, Biljana, Trifunović-Momčilov, Milana, Simonović, Ana, Jevremović, Slađana, Subotić, Angelina, "Distribution of some arabinogalactan protein epitopes during somatic embryogenesis and organogenesis on leaf explants on centaury (Centaurium erythraea Rafn)" in 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade (2018):21-22,
https://hdl.handle.net/21.15107/rcub_ibiss_4387 .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Paunović, Danijela
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4338
AB  - Impatiens walleriana is an important ornamental plant with a tendency to quickly wilt when
exposed to dehydration, which considerably affects its commercial value. However, Impatiens also
has great potential to rehydrate upon watering. Effective protection of this valuable ornamental
from water stress requires understanding the events associated with dehydration and rehydration
at the molecular level. Dehydrins (DHNs) are highly hydrophilic proteins whose expression in many
plant species has been correlated with several types of abiotic stresses, including drought, salinity,
and cold. Dehydrins comprise Group II of late embryogenesis abundant (LEA) proteins, known to
be associated with plant responses to water deficit. DHNs lack stable tridimensional structures, so
they are considered intrinsically disordered proteins. They are characterized by three conserved
sequence motifs marked as a K (Lys-rich), Y (Tyr-rich) and S (Ser-rich). To study the expression of
Dhn genes in response to drought, we have sequenced I. walleriana transcriptome and identified
IwDhn transcripts from RNA-seq data using HMMER (hmmscan, v3.1b2) and Pfam 31 database.
Quantitative RT-PCR revealed that two IwDhn genes are extremely upregulated in drought, while
one has constitutive expression. It can be speculated that fast rehydration after drought stress is at
least in part result of the protective role of dehydrins.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Expression of dehydrins in Impatiens walleriana exposed to drought
SP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4338
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Dragićević, Milan and Simonović, Ana and Paunović, Danijela and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is an important ornamental plant with a tendency to quickly wilt when
exposed to dehydration, which considerably affects its commercial value. However, Impatiens also
has great potential to rehydrate upon watering. Effective protection of this valuable ornamental
from water stress requires understanding the events associated with dehydration and rehydration
at the molecular level. Dehydrins (DHNs) are highly hydrophilic proteins whose expression in many
plant species has been correlated with several types of abiotic stresses, including drought, salinity,
and cold. Dehydrins comprise Group II of late embryogenesis abundant (LEA) proteins, known to
be associated with plant responses to water deficit. DHNs lack stable tridimensional structures, so
they are considered intrinsically disordered proteins. They are characterized by three conserved
sequence motifs marked as a K (Lys-rich), Y (Tyr-rich) and S (Ser-rich). To study the expression of
Dhn genes in response to drought, we have sequenced I. walleriana transcriptome and identified
IwDhn transcripts from RNA-seq data using HMMER (hmmscan, v3.1b2) and Pfam 31 database.
Quantitative RT-PCR revealed that two IwDhn genes are extremely upregulated in drought, while
one has constitutive expression. It can be speculated that fast rehydration after drought stress is at
least in part result of the protective role of dehydrins.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Expression of dehydrins in Impatiens walleriana exposed to drought",
pages = "74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4338"
}

Antonić, D., Milošević, S., Dragićević, M., Simonović, A., Paunović, D., Momčilović, I.,& Subotić, A.. (2018). Expression of dehydrins in Impatiens walleriana exposed to drought. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 74.
https://hdl.handle.net/21.15107/rcub_ibiss_4338

Antonić D, Milošević S, Dragićević M, Simonović A, Paunović D, Momčilović I, Subotić A. Expression of dehydrins in Impatiens walleriana exposed to drought. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:74.
https://hdl.handle.net/21.15107/rcub_ibiss_4338 .

Antonić, Dragana, Milošević, Snežana, Dragićević, Milan, Simonović, Ana, Paunović, Danijela, Momčilović, Ivana, Subotić, Angelina, "Expression of dehydrins in Impatiens walleriana exposed to drought" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):74,
https://hdl.handle.net/21.15107/rcub_ibiss_4338 .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Pantelić, Danijel
AU  - Simonović, Ana
AU  - Trajković, Milena
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4337
AB  - Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4337
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Pantelić, Danijel and Simonović, Ana and Trajković, Milena and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4337"
}

Antonić, D., Milošević, S., Pantelić, D., Simonović, A., Trajković, M., Momčilović, I.,& Subotić, A.. (2018). Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337

Antonić D, Milošević S, Pantelić D, Simonović A, Trajković M, Momčilović I, Subotić A. Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

Antonić, Dragana, Milošević, Snežana, Pantelić, Danijel, Simonović, Ana, Trajković, Milena, Momčilović, Ivana, Subotić, Angelina, "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Todorović, Slađana
AU  - Subotić, Angelina
AU  - Simonović, Ana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4335
AB  - Centaurium erythraea Rafn (Gentianaceae) is a medicinal plant, rich in secondary metabolites,
mainly secoiridoid glucosides and xanthones, known for numerous beneficial effects on human
health. In addition, C. erythraea possesses remarkable developmental plasticity and is easily cultured
in vitro, so it represents a suitable model system for studying developmental biology. One
of the most notable illustrations of plant cell totipotency is the capability of certain somatic plant
cells to initiate embryogenic development through somatic embryogenesis (SE). It has been proposed
that mechanical wounding during in vitro manipulations of plant material can promote cell
differentiation and somatic embryo development. Our recent research is based on elucidating
the gene expression profiles of centaury tissues in response to wounding and in different SE stages
using quantitative real-time PCR. Considering the fact that these processes exhibit a dynamic
genetic activity, the selection of stable reference genes is paramount in order to obtain unbiased
conclusions. Hereby we report selection of stable reference genes in C. erythraea for studying gene
expression during somatic embryogenesis and wounding. Thirteen frequently used reference
genes were selected and their expression stability was assessed in different developmental stages,
including globular and cotyledonary stages of embryos, as well as wounded tissues. Specific sets
of primers were designed relying on previously obtained next-generation RNA sequencing data.
The results were interpreted using two algorithmic approaches - geNorm and NormFinder - and
the most stable reference genes from a set of tested candidate genes were determined.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4335
ER  - 

@conference{
author = "Ćuković, Katarina and Paunović, Danijela and Bogdanović, Milica and Dragićević, Milan and Todorović, Slađana and Subotić, Angelina and Simonović, Ana",
year = "2018",
abstract = "Centaurium erythraea Rafn (Gentianaceae) is a medicinal plant, rich in secondary metabolites,
mainly secoiridoid glucosides and xanthones, known for numerous beneficial effects on human
health. In addition, C. erythraea possesses remarkable developmental plasticity and is easily cultured
in vitro, so it represents a suitable model system for studying developmental biology. One
of the most notable illustrations of plant cell totipotency is the capability of certain somatic plant
cells to initiate embryogenic development through somatic embryogenesis (SE). It has been proposed
that mechanical wounding during in vitro manipulations of plant material can promote cell
differentiation and somatic embryo development. Our recent research is based on elucidating
the gene expression profiles of centaury tissues in response to wounding and in different SE stages
using quantitative real-time PCR. Considering the fact that these processes exhibit a dynamic
genetic activity, the selection of stable reference genes is paramount in order to obtain unbiased
conclusions. Hereby we report selection of stable reference genes in C. erythraea for studying gene
expression during somatic embryogenesis and wounding. Thirteen frequently used reference
genes were selected and their expression stability was assessed in different developmental stages,
including globular and cotyledonary stages of embryos, as well as wounded tissues. Specific sets
of primers were designed relying on previously obtained next-generation RNA sequencing data.
The results were interpreted using two algorithmic approaches - geNorm and NormFinder - and
the most stable reference genes from a set of tested candidate genes were determined.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4335"
}

Ćuković, K., Paunović, D., Bogdanović, M., Dragićević, M., Todorović, S., Subotić, A.,& Simonović, A.. (2018). Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_4335

Ćuković K, Paunović D, Bogdanović M, Dragićević M, Todorović S, Subotić A, Simonović A. Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_4335 .

Ćuković, Katarina, Paunović, Danijela, Bogdanović, Milica, Dragićević, Milan, Todorović, Slađana, Subotić, Angelina, Simonović, Ana, "Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):28,
https://hdl.handle.net/21.15107/rcub_ibiss_4335 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Subotić, Angelina
AU  - Todorović, Slađana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4336
AB  - Centaurium erythraea Rafn is a widespread medicinal plant from the Gentianaceae family.
Grown in vitro, centaury displays enviable developmental plasticity, often being capable of regenerating
the whole plant from root or shoot tissues through pathways of organogenesis and
somatic embryogenesis. Somatic embryogenesis (SE) is especially interesting for mass plant propagation,
production of virus-free material, synthetic seeds and cryopreservation, and is known to
produce less somaclonal variation than organogenesis. In Centaurium, both pathways have been
reported to occur both spontaneously and induced by plant growth regulators (2,4-D and CPPU).
It has been noted that subculturing of embryogenic callus for multiplication over extended period
of time results in the reduction of embryogenic potential. One way to overcome this problem is
initiation of secondary embryogenesis from primary cotyledonary embryos. Here we report successful
secondary embryogenesis in C. erythraea induced both directly and indirectly from cotyledonary
somatic embryos. Several combinations of different 2,4-D and CPPU concentrations were
tested for efficiency to initiate secondary embryogenesis in the dark. After four weeks, several
parameters were recorded: presence of callus and new cotyledonary embryos, number of cotyledonary
embryos per explant and whether new embryos were formed directly or indirectly. Secondary
embryos were capable of producing tertiary somatic embryos, effectively entering cyclic
SE. Secondary embryos, as well as primary, were able to germinate into plantlets by transferring
them on hormone-free medium.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Secondary somatic embryogenesis in Centaurium erythraea
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4336
ER  - 

@conference{
author = "Bogdanović, Milica and Ćuković, Katarina and Dragićević, Milan and Simonović, Ana and Subotić, Angelina and Todorović, Slađana",
year = "2018",
abstract = "Centaurium erythraea Rafn is a widespread medicinal plant from the Gentianaceae family.
Grown in vitro, centaury displays enviable developmental plasticity, often being capable of regenerating
the whole plant from root or shoot tissues through pathways of organogenesis and
somatic embryogenesis. Somatic embryogenesis (SE) is especially interesting for mass plant propagation,
production of virus-free material, synthetic seeds and cryopreservation, and is known to
produce less somaclonal variation than organogenesis. In Centaurium, both pathways have been
reported to occur both spontaneously and induced by plant growth regulators (2,4-D and CPPU).
It has been noted that subculturing of embryogenic callus for multiplication over extended period
of time results in the reduction of embryogenic potential. One way to overcome this problem is
initiation of secondary embryogenesis from primary cotyledonary embryos. Here we report successful
secondary embryogenesis in C. erythraea induced both directly and indirectly from cotyledonary
somatic embryos. Several combinations of different 2,4-D and CPPU concentrations were
tested for efficiency to initiate secondary embryogenesis in the dark. After four weeks, several
parameters were recorded: presence of callus and new cotyledonary embryos, number of cotyledonary
embryos per explant and whether new embryos were formed directly or indirectly. Secondary
embryos were capable of producing tertiary somatic embryos, effectively entering cyclic
SE. Secondary embryos, as well as primary, were able to germinate into plantlets by transferring
them on hormone-free medium.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Secondary somatic embryogenesis in Centaurium erythraea",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4336"
}

Bogdanović, M., Ćuković, K., Dragićević, M., Simonović, A., Subotić, A.,& Todorović, S.. (2018). Secondary somatic embryogenesis in Centaurium erythraea. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 34.
https://hdl.handle.net/21.15107/rcub_ibiss_4336

Bogdanović M, Ćuković K, Dragićević M, Simonović A, Subotić A, Todorović S. Secondary somatic embryogenesis in Centaurium erythraea. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:34.
https://hdl.handle.net/21.15107/rcub_ibiss_4336 .

Bogdanović, Milica, Ćuković, Katarina, Dragićević, Milan, Simonović, Ana, Subotić, Angelina, Todorović, Slađana, "Secondary somatic embryogenesis in Centaurium erythraea" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):34,
https://hdl.handle.net/21.15107/rcub_ibiss_4336 .

TY  - CONF
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Trifunović-Momčilov, Milana
AU  - Todorović, Slađana
AU  - Simonović, Ana
AU  - Subotić, Angelina
AU  - Dragićević, Milan
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4342
AB  - Arabinogalactan proteins (AGPs) are cell wall proteoglycans with important roles during plant
growth and development. They comprise one of the most complex families of macromolecules
found in plants, which is in part due to the incredible diversity of the glycans decorating the pro-
tein backbone, as well as the heterogeneity of the protein backbones. While this diversity is cer-
tainly responsible for the wide array of physiological functions associated with AGPs, it hampers
efforts for homology-based identification. We have developed a new method for filtering AGP se-
quences that exploits one of their key features, the presence of hydroxyprolines, which represent
glycosylation sites. This method was used to filter potential AGPs from Centaurium erythraea RNA-
seq data. Most of the filtered sequences had no identifiable domains, while the most frequent
identified domains were the Protein kinase and Protein tyrosine kinase domains identified in the
same sequences, followed by well-known AGP associates, Leucine rich repeats, Probable lipid
transfer, Plastocyanin-like and Fasciclin. It is noteworthy that the Protein (tyrosine) kinase domain
has thus far eluded experimental evidence for linkage with AGPs in any plant species, probably
due to its transmembrane nature. The implicated sequences were examined in depth and com-
pared to homologs from Arabidopsis.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts, 3rd International Conference on Plant biology, 9.-12. June 2018., Belgrade, Serbia
T1  - Are receptor tyrosine kinases chimeric AGP’s?
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4342
ER  - 

@conference{
author = "Paunović, Danijela and Bogdanović, Milica and Trifunović-Momčilov, Milana and Todorović, Slađana and Simonović, Ana and Subotić, Angelina and Dragićević, Milan",
year = "2018",
abstract = "Arabinogalactan proteins (AGPs) are cell wall proteoglycans with important roles during plant
growth and development. They comprise one of the most complex families of macromolecules
found in plants, which is in part due to the incredible diversity of the glycans decorating the pro-
tein backbone, as well as the heterogeneity of the protein backbones. While this diversity is cer-
tainly responsible for the wide array of physiological functions associated with AGPs, it hampers
efforts for homology-based identification. We have developed a new method for filtering AGP se-
quences that exploits one of their key features, the presence of hydroxyprolines, which represent
glycosylation sites. This method was used to filter potential AGPs from Centaurium erythraea RNA-
seq data. Most of the filtered sequences had no identifiable domains, while the most frequent
identified domains were the Protein kinase and Protein tyrosine kinase domains identified in the
same sequences, followed by well-known AGP associates, Leucine rich repeats, Probable lipid
transfer, Plastocyanin-like and Fasciclin. It is noteworthy that the Protein (tyrosine) kinase domain
has thus far eluded experimental evidence for linkage with AGPs in any plant species, probably
due to its transmembrane nature. The implicated sequences were examined in depth and com-
pared to homologs from Arabidopsis.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts, 3rd International Conference on Plant biology, 9.-12. June 2018., Belgrade, Serbia",
title = "Are receptor tyrosine kinases chimeric AGP’s?",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4342"
}

Paunović, D., Bogdanović, M., Trifunović-Momčilov, M., Todorović, S., Simonović, A., Subotić, A.,& Dragićević, M.. (2018). Are receptor tyrosine kinases chimeric AGP’s?. in Book of Abstracts, 3rd International Conference on Plant biology, 9.-12. June 2018., Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society..
https://hdl.handle.net/21.15107/rcub_ibiss_4342

Paunović D, Bogdanović M, Trifunović-Momčilov M, Todorović S, Simonović A, Subotić A, Dragićević M. Are receptor tyrosine kinases chimeric AGP’s?. in Book of Abstracts, 3rd International Conference on Plant biology, 9.-12. June 2018., Belgrade, Serbia. 2018;.
https://hdl.handle.net/21.15107/rcub_ibiss_4342 .

Paunović, Danijela, Bogdanović, Milica, Trifunović-Momčilov, Milana, Todorović, Slađana, Simonović, Ana, Subotić, Angelina, Dragićević, Milan, "Are receptor tyrosine kinases chimeric AGP’s?" in Book of Abstracts, 3rd International Conference on Plant biology, 9.-12. June 2018., Belgrade, Serbia (2018),
https://hdl.handle.net/21.15107/rcub_ibiss_4342 .

TY  - CONF
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6731
AB  - Introduction: Viola cornuta is a valuable perennial ornamental plant. Development of new traits, such as new flower color with classical breeding suffers from many difficulties, which can be overcome using genetic engineering. With aim to develop protocol for Agrobacterium-mediated transformation of V. cornuta, we used Agrobacterium tumefaciens strain LBA4404 harbouring the superbinary vector pTOK233 carried a GUS reporter gene and hygromycin phosphotransferase selectable marker gene.
Methods: Hypocotyl explants obtained from seedlings were grown on ½MS medium supplemented with 0.1 mg/l 2,4-D and 2.0 mg/l BA for shoot induction. After two days of pre-cultivation, hypocotyl explants were inoculated in bacterial suspension for 15 min and placed on the same culture medium with addition of acetosyringone 100 μM at pH 5.2. After two days of co-cultivation, explants were transferred on shoot induction medium supplemented with cefotaxime and hygromycin B for selection. Regenerated putative transformants were analyzed by PCR for hygromycin phsphotransferase gene presence and by histochemical assay for β-glucuronidase (GUS) activity.
Results: Shoots were obtained within 8 weeks after explants were inoculated with A. tumefaciens, with 2.0% regeneration efficiency. PCR analysis confirmed selectable marker gene presence in twelve out of sixteen (75.0%) independently derived putatively transformed lines that were tested. Additionally, all analyzed lines exhibited a notable β-glucuronidase activity that was not present in untransformed plants.
Conclusion: This is the first report about V. cornuta susceptibility to A. tumefaciens. Presented protocol for genetic transformation can be used for further introduction of desirable traits in V. cornuta cultivars.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
T1  - Agrobacterium-mediated genetic transformation of Viola cornuta
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6731
ER  - 

@conference{
author = "Trajković, Milena and Jeknić, Zoran and Antonić Reljin, Dragana and Subotić, Angelina and Jevremović, Slađana and Cingel, Aleksandar",
year = "2017",
abstract = "Introduction: Viola cornuta is a valuable perennial ornamental plant. Development of new traits, such as new flower color with classical breeding suffers from many difficulties, which can be overcome using genetic engineering. With aim to develop protocol for Agrobacterium-mediated transformation of V. cornuta, we used Agrobacterium tumefaciens strain LBA4404 harbouring the superbinary vector pTOK233 carried a GUS reporter gene and hygromycin phosphotransferase selectable marker gene.
Methods: Hypocotyl explants obtained from seedlings were grown on ½MS medium supplemented with 0.1 mg/l 2,4-D and 2.0 mg/l BA for shoot induction. After two days of pre-cultivation, hypocotyl explants were inoculated in bacterial suspension for 15 min and placed on the same culture medium with addition of acetosyringone 100 μM at pH 5.2. After two days of co-cultivation, explants were transferred on shoot induction medium supplemented with cefotaxime and hygromycin B for selection. Regenerated putative transformants were analyzed by PCR for hygromycin phsphotransferase gene presence and by histochemical assay for β-glucuronidase (GUS) activity.
Results: Shoots were obtained within 8 weeks after explants were inoculated with A. tumefaciens, with 2.0% regeneration efficiency. PCR analysis confirmed selectable marker gene presence in twelve out of sixteen (75.0%) independently derived putatively transformed lines that were tested. Additionally, all analyzed lines exhibited a notable β-glucuronidase activity that was not present in untransformed plants.
Conclusion: This is the first report about V. cornuta susceptibility to A. tumefaciens. Presented protocol for genetic transformation can be used for further introduction of desirable traits in V. cornuta cultivars.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia",
title = "Agrobacterium-mediated genetic transformation of Viola cornuta",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6731"
}

Trajković, M., Jeknić, Z., Antonić Reljin, D., Subotić, A., Jevremović, S.,& Cingel, A.. (2017). Agrobacterium-mediated genetic transformation of Viola cornuta. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 83.
https://hdl.handle.net/21.15107/rcub_ibiss_6731

Trajković M, Jeknić Z, Antonić Reljin D, Subotić A, Jevremović S, Cingel A. Agrobacterium-mediated genetic transformation of Viola cornuta. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. 2017;:83.
https://hdl.handle.net/21.15107/rcub_ibiss_6731 .

Trajković, Milena, Jeknić, Zoran, Antonić Reljin, Dragana, Subotić, Angelina, Jevremović, Slađana, Cingel, Aleksandar, "Agrobacterium-mediated genetic transformation of Viola cornuta" in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia (2017):83,
https://hdl.handle.net/21.15107/rcub_ibiss_6731 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2017
UR  - http://www.journal-pop.org/2017_17_3_95-102.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2905
AB  - In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.
T2  - Propagation of Ornamental Plants
T1  - Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'
IS  - 3
VL  - 17
SP  - 95
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2905
ER  - 

@article{
author = "Antonić Reljin, Dragana and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2017",
abstract = "In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.",
journal = "Propagation of Ornamental Plants",
title = "Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'",
number = "3",
volume = "17",
pages = "95-102",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2905"
}

Antonić Reljin, D., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2017). Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'. in Propagation of Ornamental Plants, 17(3), 95-102.
https://hdl.handle.net/21.15107/rcub_ibiss_2905

Antonić Reljin D, Trajković M, Cingel A, Subotić A, Jevremović S. Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'. in Propagation of Ornamental Plants. 2017;17(3):95-102.
https://hdl.handle.net/21.15107/rcub_ibiss_2905 .

Antonić Reljin, Dragana, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'" in Propagation of Ornamental Plants, 17, no. 3 (2017):95-102,
https://hdl.handle.net/21.15107/rcub_ibiss_2905 .

TY  - CONF
AU  - Simonović, Ana
AU  - Dragićević, Milan
AU  - Giurato, Giorgio
AU  - Filipović, Biljana
AU  - Todorović, Slađana
AU  - Bogdanović, Milica
AU  - Ćuković, Katarina
AU  - Subotić, Angelina
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6157
AB  - Centaurium erythraea is a n endangered medicinal plant with great regeneration potential and developmental plasticity in vitro [1]. Identification of genes involved in organogenesis and somatic embryogenesis (SE) is the first step towards elucidation of molecular mechanisms underlying centaurys morphogenic plasticity. RNA from leaves (L), roots (R), embryogenic calii (EC), globular somatic embryos (GSE), cotyledonary somatic embryos (CSE) and adventitious buds (AB) was sequenced, resulting in 29-37 million reads/sample. Sequencing, de novo transcriptome assembly using Trinity and annotation were operated by Genomix4Life. The reference transcriptome (142 Mbp) contained 160,839 Trinity transcripts comprising 105,726 "genes". Of 160,839 transcripts, 44,288 had Blast hits, 26,435 had GO Slim annotation, whereas 9,552 were with GO mapping. The top-hit species was Coffea canephora. Relative expression was computed by aligning high quality reads to the Trinity transcripts and presented as TMM-FPKM. In each sample >=30,000 transcripts were expressed. Transcripts involved in different morphogenetic paths were filtered using R. Potential SE markers (FPKM >=1 in EC or GSE and >=8X higher FPKM in EC or GSE than in L, R and AB) included 1989 sequences, such as LRR receptor-like PK, germin-like proteins, TFs WRKY, AINTEGUMENTA and others. There were 1203 transcripts important for later SE development, including seed storage proteins and expansins. Finally, 727 transcripts with at least 8x higher FPKM in AB than in other samples were considered as important for organogenesis.
PB  - Belgrade: Faculty of Mathematics, University
C3  - Book of Abstracts: Belgrade Bioinformatics Conference: BelBI2016; 2016 Jun 20-24; Belgrade, Serbia
T1  - Identification of genes involved in morphogenesis in vitro in Centaurium erythraea Rafn. as a model organism
SP  - 97
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6157
ER  - 

@conference{
author = "Simonović, Ana and Dragićević, Milan and Giurato, Giorgio and Filipović, Biljana and Todorović, Slađana and Bogdanović, Milica and Ćuković, Katarina and Subotić, Angelina",
year = "2016",
abstract = "Centaurium erythraea is a n endangered medicinal plant with great regeneration potential and developmental plasticity in vitro [1]. Identification of genes involved in organogenesis and somatic embryogenesis (SE) is the first step towards elucidation of molecular mechanisms underlying centaurys morphogenic plasticity. RNA from leaves (L), roots (R), embryogenic calii (EC), globular somatic embryos (GSE), cotyledonary somatic embryos (CSE) and adventitious buds (AB) was sequenced, resulting in 29-37 million reads/sample. Sequencing, de novo transcriptome assembly using Trinity and annotation were operated by Genomix4Life. The reference transcriptome (142 Mbp) contained 160,839 Trinity transcripts comprising 105,726 "genes". Of 160,839 transcripts, 44,288 had Blast hits, 26,435 had GO Slim annotation, whereas 9,552 were with GO mapping. The top-hit species was Coffea canephora. Relative expression was computed by aligning high quality reads to the Trinity transcripts and presented as TMM-FPKM. In each sample >=30,000 transcripts were expressed. Transcripts involved in different morphogenetic paths were filtered using R. Potential SE markers (FPKM >=1 in EC or GSE and >=8X higher FPKM in EC or GSE than in L, R and AB) included 1989 sequences, such as LRR receptor-like PK, germin-like proteins, TFs WRKY, AINTEGUMENTA and others. There were 1203 transcripts important for later SE development, including seed storage proteins and expansins. Finally, 727 transcripts with at least 8x higher FPKM in AB than in other samples were considered as important for organogenesis.",
publisher = "Belgrade: Faculty of Mathematics, University",
journal = "Book of Abstracts: Belgrade Bioinformatics Conference: BelBI2016; 2016 Jun 20-24; Belgrade, Serbia",
title = "Identification of genes involved in morphogenesis in vitro in Centaurium erythraea Rafn. as a model organism",
pages = "97-98",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6157"
}

Simonović, A., Dragićević, M., Giurato, G., Filipović, B., Todorović, S., Bogdanović, M., Ćuković, K.,& Subotić, A.. (2016). Identification of genes involved in morphogenesis in vitro in Centaurium erythraea Rafn. as a model organism. in Book of Abstracts: Belgrade Bioinformatics Conference: BelBI2016; 2016 Jun 20-24; Belgrade, Serbia
Belgrade: Faculty of Mathematics, University., 97-98.
https://hdl.handle.net/21.15107/rcub_ibiss_6157

Simonović A, Dragićević M, Giurato G, Filipović B, Todorović S, Bogdanović M, Ćuković K, Subotić A. Identification of genes involved in morphogenesis in vitro in Centaurium erythraea Rafn. as a model organism. in Book of Abstracts: Belgrade Bioinformatics Conference: BelBI2016; 2016 Jun 20-24; Belgrade, Serbia. 2016;:97-98.
https://hdl.handle.net/21.15107/rcub_ibiss_6157 .

Simonović, Ana, Dragićević, Milan, Giurato, Giorgio, Filipović, Biljana, Todorović, Slađana, Bogdanović, Milica, Ćuković, Katarina, Subotić, Angelina, "Identification of genes involved in morphogenesis in vitro in Centaurium erythraea Rafn. as a model organism" in Book of Abstracts: Belgrade Bioinformatics Conference: BelBI2016; 2016 Jun 20-24; Belgrade, Serbia (2016):97-98,
https://hdl.handle.net/21.15107/rcub_ibiss_6157 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Trajković, Milena
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Subotić, Angelina
AU  - Simonović, Ana
PY  - 2016
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84963773069&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2470
UR  - https://www.sciencedirect.com/science/article/pii/S0254629915326594?via%3Dihub
AB  - We describe the responses of Impatiens walleriana to polyethylene glycol (PEG)-induced physiological drought and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent. Impatiens shoot culture was established on 16 different media containing 0-3% PEG and 0-3 mM SA. After prolonged drought (60 days), water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight, the number of developed leaves and shoots (proliferation rate, PR), relative water content, and chlorophyll content. PEG increased leaf water loss (LWL) and caused accumulation of proline, H2O2, and malondialdehyde. The activities of catalase, superoxide dismutase, and peroxidase were increased in response to PEG in a dose-dependent manner, with specific peroxidase isoforms induced by drought. Exogenous SA counteracted the effects of PEG on growth, physiological and biochemical parameters, except on proline accumulation. SA was particularly effective in enhancing PR, preserving LWL, and protecting photosynthetic pigments and membranes from oxidative damage. Proline accumulation was strongly enhanced by both PEG and SA. SA had differential effects on different peroxidase isoforms. SA may be safely used in 2-3 mM concentration for drought protection of Impatiens with no negative effects.
PB  - Elsevier
T2  - South African Journal of Botany
T1  - Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought
VL  - 105
DO  - 10.1016/j.sajb.2016.04.002
SP  - 226
EP  - 233
ER  - 

@article{
author = "Antonić Reljin, Dragana and Milošević, Snežana and Cingel, Aleksandar and Trajković, Milena and Trifunović-Momčilov, Milana and Marković, Marija and Subotić, Angelina and Simonović, Ana",
year = "2016",
abstract = "We describe the responses of Impatiens walleriana to polyethylene glycol (PEG)-induced physiological drought and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent. Impatiens shoot culture was established on 16 different media containing 0-3% PEG and 0-3 mM SA. After prolonged drought (60 days), water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight, the number of developed leaves and shoots (proliferation rate, PR), relative water content, and chlorophyll content. PEG increased leaf water loss (LWL) and caused accumulation of proline, H2O2, and malondialdehyde. The activities of catalase, superoxide dismutase, and peroxidase were increased in response to PEG in a dose-dependent manner, with specific peroxidase isoforms induced by drought. Exogenous SA counteracted the effects of PEG on growth, physiological and biochemical parameters, except on proline accumulation. SA was particularly effective in enhancing PR, preserving LWL, and protecting photosynthetic pigments and membranes from oxidative damage. Proline accumulation was strongly enhanced by both PEG and SA. SA had differential effects on different peroxidase isoforms. SA may be safely used in 2-3 mM concentration for drought protection of Impatiens with no negative effects.",
publisher = "Elsevier",
journal = "South African Journal of Botany",
title = "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought",
volume = "105",
doi = "10.1016/j.sajb.2016.04.002",
pages = "226-233"
}

Antonić Reljin, D., Milošević, S., Cingel, A., Trajković, M., Trifunović-Momčilov, M., Marković, M., Subotić, A.,& Simonović, A.. (2016). Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. in South African Journal of Botany
Elsevier., 105, 226-233.
https://doi.org/10.1016/j.sajb.2016.04.002

Antonić Reljin D, Milošević S, Cingel A, Trajković M, Trifunović-Momčilov M, Marković M, Subotić A, Simonović A. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. in South African Journal of Botany. 2016;105:226-233.
doi:10.1016/j.sajb.2016.04.002 .

Antonić Reljin, Dragana, Milošević, Snežana, Cingel, Aleksandar, Trajković, Milena, Trifunović-Momčilov, Milana, Marković, Marija, Subotić, Angelina, Simonović, Ana, "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought" in South African Journal of Botany, 105 (2016):226-233,
https://doi.org/10.1016/j.sajb.2016.04.002 . .

TY  - JOUR
AU  - Simonović, Ana
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
AU  - Todorović, Slađana
PY  - 2016
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600023S
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1245
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-85002620678&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=FBB4104D27B7BC3C905C7DEC47444824.wsnAw8kcdt7IPYLO0V48gA%3A87
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2487
AB  - Over 20% of all protein domains are currently annotated as "domains of unknown function" or DUFs. In a recently identified Centaurium erythraea arabinogalactan peptide, CeAGP3 (AGN92423), a conserved DUF1070 domain was found. Since identifying functions for DUFs is important in systems biology, we have analyzed the distribution and structure of DUF1070 domain (pfam06376) using a set of bioinformatics tools. There are 271 publically available DUF1070 members from 25 diverse families of vascular plants, and most are short sequences (50-100 aa). The N-terminal signal peptide (Nsp) was found in almost all complete sequences. In 233 sequences, at least two noncontiguous prolines were found as clustered dipeptides predicted to be hydroxylated and glycosylated with type II arabino-3,6-galactans, thus representing AG-II glycomodules. In addition, 35 sequences contained a region rich in basic residues (basic linker, BL). The N-terminal part of the DUF1070 domain is comprised of (part of) AG-II and/or BL, while the highly conserved C-terminus is a region of 26 aa, termed SH26. In 212 sequences, SH26 was a typical glycosylphosphatidylinositol lipid anchor signal peptide (GPIsp), but in 83 cases GPIsp was not predicted due to software constraints. In sequences where both Nsp and GPIsp were predicted, the length of mature peptides could be calculated, and it was 10-16 aa. Our analysis suggests that DUF1070 members are arabinogalactan (AG) peptides, of which the majority are GPI-anchored. DUF1070 is the only conserved domain found in classical arabinogalactan proteins and AG peptides. The SH26 region can be used for mining and annotation of AG peptides.
T2  - Archives of Biological Sciences
T1  - DUF1070 as a signature domain of a subclass of arabinogalactan peptides
IS  - 4
VL  - 68
DO  - 10.2298/ABS151120023S
SP  - 737
EP  - 746
ER  - 

@article{
author = "Simonović, Ana and Dragićević, Milan and Bogdanović, Milica and Trifunović-Momčilov, Milana and Subotić, Angelina and Todorović, Slađana",
year = "2016",
abstract = "Over 20% of all protein domains are currently annotated as "domains of unknown function" or DUFs. In a recently identified Centaurium erythraea arabinogalactan peptide, CeAGP3 (AGN92423), a conserved DUF1070 domain was found. Since identifying functions for DUFs is important in systems biology, we have analyzed the distribution and structure of DUF1070 domain (pfam06376) using a set of bioinformatics tools. There are 271 publically available DUF1070 members from 25 diverse families of vascular plants, and most are short sequences (50-100 aa). The N-terminal signal peptide (Nsp) was found in almost all complete sequences. In 233 sequences, at least two noncontiguous prolines were found as clustered dipeptides predicted to be hydroxylated and glycosylated with type II arabino-3,6-galactans, thus representing AG-II glycomodules. In addition, 35 sequences contained a region rich in basic residues (basic linker, BL). The N-terminal part of the DUF1070 domain is comprised of (part of) AG-II and/or BL, while the highly conserved C-terminus is a region of 26 aa, termed SH26. In 212 sequences, SH26 was a typical glycosylphosphatidylinositol lipid anchor signal peptide (GPIsp), but in 83 cases GPIsp was not predicted due to software constraints. In sequences where both Nsp and GPIsp were predicted, the length of mature peptides could be calculated, and it was 10-16 aa. Our analysis suggests that DUF1070 members are arabinogalactan (AG) peptides, of which the majority are GPI-anchored. DUF1070 is the only conserved domain found in classical arabinogalactan proteins and AG peptides. The SH26 region can be used for mining and annotation of AG peptides.",
journal = "Archives of Biological Sciences",
title = "DUF1070 as a signature domain of a subclass of arabinogalactan peptides",
number = "4",
volume = "68",
doi = "10.2298/ABS151120023S",
pages = "737-746"
}

Simonović, A., Dragićević, M., Bogdanović, M., Trifunović-Momčilov, M., Subotić, A.,& Todorović, S.. (2016). DUF1070 as a signature domain of a subclass of arabinogalactan peptides. in Archives of Biological Sciences, 68(4), 737-746.
https://doi.org/10.2298/ABS151120023S

Simonović A, Dragićević M, Bogdanović M, Trifunović-Momčilov M, Subotić A, Todorović S. DUF1070 as a signature domain of a subclass of arabinogalactan peptides. in Archives of Biological Sciences. 2016;68(4):737-746.
doi:10.2298/ABS151120023S .

Simonović, Ana, Dragićević, Milan, Bogdanović, Milica, Trifunović-Momčilov, Milana, Subotić, Angelina, Todorović, Slađana, "DUF1070 as a signature domain of a subclass of arabinogalactan peptides" in Archives of Biological Sciences, 68, no. 4 (2016):737-746,
https://doi.org/10.2298/ABS151120023S . .

TY  - JOUR
AU  - Jevremović, Slađana
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Trifunović-Momčilov, Milana
AU  - Antonić Reljin, Dragana
AU  - Marković, Marija
AU  - Subotić, Angelina
AU  - Radojević, Ljiljana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6411
AB  - An efficient propagation protocol by somatic embryogenesis and organogenesis of Balkan endemic iris, Iris reichenbachii, was achieved and clonal fidelity of regenerated plants evaluated. Both regeneration pathways were induced at the same time in zygotic embryo culture on Murashige & Skoog (MS) medium supplemented with 0.5-5.0 mM 2,4-dichlorophenoxy acetic acid (2,4-D) as the sole hormone. Embryogenic calli were further mantained on medium supplemented with 2,4-D and kinetin (Kn; 0.5 and 5.0 mM, respectively). Organogenic calli were selected and further cultured on MS media supplemented with 1-naphthaleneacetic acid (NAA) and benzyladenine (BA; 0.5 and 4.5 mM, respectively) for shoot initiation. Somatic embryos germinated and shoots rooted on MS plant growth regulator-free medium. Plants regenerated by both procceses were succesfully acclimatized in greenhouse conditions and flowered in the following flowering season. Some alterations in flower morphology were detected among plants regenerated by organogenesis. Flow cytometric analysis revealed that plants with altered morphology of flowers had the same ploidy level and genome size as plants collected from the natural habitat. A tetraploid plant was observed in the population of plants regenerated by somatic embryogenesis induced at a high concentration of 2,4-D (10.0 mM).
AB  - Prikazan je efikasan protokol za propagaciju somatskom embriogenezom i organogenezom Balkanske endemične perunike, Iris reichenbachii i procenjena je klonalna indentičnost dobijenih biljaka. Oba načina za regeneraciju u uslovima in vitro su postignuta u kulturi zrelih zigotskih embriona na Murashige & Skoog (MS) hranljivoj podlozi obogaćenoj sa 2,4-dihlorofenoksi sirćetnom kiselinom 0.5-5.0 mM (2,4-D) kao jedinim regulatorom rastenja. Dobijeni embriogeni kalusi su dalje gajeni na hranljivoj podlozi sa 2,4-D i kinetinom (Kn; 0.5 odnosno 5.0 mM). Formirani organogeni kalusi su dalje gajeni na MS hranljivoj podlozi obogaćenoj sa α-naftilsirćetnom kiselinom (NAA) i benziladeninom (BA; 0.5 odnosno 4.5 mM) kada je došlo do formiranja izdanaka. Klijanje somatskih embriona kao i ožiljavanje formiranih izdanaka postignuto je na MS hranljivoj podlozi bez biljnih regulatora rastenja. Biljke dobijene na oba načina su dalje uspešno aklimatizovane na uslove gajenja u stakleniku i cvetale su sledeće godine u proleće. Uočene su neke promene u morfologiji cvetova kod biljaka dobijenih procesom organogeneze. Na osnovu flow-citometrijske analize pokazano je da su biljke sa izmenjenom morfologijom cvetova imale isti nivo ploidnosti i veličinu genoma kao biljke iz prirode. U populaciji biljaka regenerisanih procesom somatske embriogeneze koja je indukovana na podlozi sa visokom koncentracijom 2,4-D (10.0 mM) jedna biljka je bila tetraploidna.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants
T1  - In vitro propagacija Iris reichenbachii Heuff. i klonalna identičnost dobijenih biljaka
IS  - 2
VL  - 39
SP  - 129
EP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6411
ER  - 

@article{
author = "Jevremović, Slađana and Trajković, Milena and Jeknić, Zoran and Trifunović-Momčilov, Milana and Antonić Reljin, Dragana and Marković, Marija and Subotić, Angelina and Radojević, Ljiljana",
year = "2015",
abstract = "An efficient propagation protocol by somatic embryogenesis and organogenesis of Balkan endemic iris, Iris reichenbachii, was achieved and clonal fidelity of regenerated plants evaluated. Both regeneration pathways were induced at the same time in zygotic embryo culture on Murashige & Skoog (MS) medium supplemented with 0.5-5.0 mM 2,4-dichlorophenoxy acetic acid (2,4-D) as the sole hormone. Embryogenic calli were further mantained on medium supplemented with 2,4-D and kinetin (Kn; 0.5 and 5.0 mM, respectively). Organogenic calli were selected and further cultured on MS media supplemented with 1-naphthaleneacetic acid (NAA) and benzyladenine (BA; 0.5 and 4.5 mM, respectively) for shoot initiation. Somatic embryos germinated and shoots rooted on MS plant growth regulator-free medium. Plants regenerated by both procceses were succesfully acclimatized in greenhouse conditions and flowered in the following flowering season. Some alterations in flower morphology were detected among plants regenerated by organogenesis. Flow cytometric analysis revealed that plants with altered morphology of flowers had the same ploidy level and genome size as plants collected from the natural habitat. A tetraploid plant was observed in the population of plants regenerated by somatic embryogenesis induced at a high concentration of 2,4-D (10.0 mM)., Prikazan je efikasan protokol za propagaciju somatskom embriogenezom i organogenezom Balkanske endemične perunike, Iris reichenbachii i procenjena je klonalna indentičnost dobijenih biljaka. Oba načina za regeneraciju u uslovima in vitro su postignuta u kulturi zrelih zigotskih embriona na Murashige & Skoog (MS) hranljivoj podlozi obogaćenoj sa 2,4-dihlorofenoksi sirćetnom kiselinom 0.5-5.0 mM (2,4-D) kao jedinim regulatorom rastenja. Dobijeni embriogeni kalusi su dalje gajeni na hranljivoj podlozi sa 2,4-D i kinetinom (Kn; 0.5 odnosno 5.0 mM). Formirani organogeni kalusi su dalje gajeni na MS hranljivoj podlozi obogaćenoj sa α-naftilsirćetnom kiselinom (NAA) i benziladeninom (BA; 0.5 odnosno 4.5 mM) kada je došlo do formiranja izdanaka. Klijanje somatskih embriona kao i ožiljavanje formiranih izdanaka postignuto je na MS hranljivoj podlozi bez biljnih regulatora rastenja. Biljke dobijene na oba načina su dalje uspešno aklimatizovane na uslove gajenja u stakleniku i cvetale su sledeće godine u proleće. Uočene su neke promene u morfologiji cvetova kod biljaka dobijenih procesom organogeneze. Na osnovu flow-citometrijske analize pokazano je da su biljke sa izmenjenom morfologijom cvetova imale isti nivo ploidnosti i veličinu genoma kao biljke iz prirode. U populaciji biljaka regenerisanih procesom somatske embriogeneze koja je indukovana na podlozi sa visokom koncentracijom 2,4-D (10.0 mM) jedna biljka je bila tetraploidna.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants, In vitro propagacija Iris reichenbachii Heuff. i klonalna identičnost dobijenih biljaka",
number = "2",
volume = "39",
pages = "129-136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6411"
}

Jevremović, S., Trajković, M., Jeknić, Z., Trifunović-Momčilov, M., Antonić Reljin, D., Marković, M., Subotić, A.,& Radojević, L.. (2015). In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 39(2), 129-136.
https://hdl.handle.net/21.15107/rcub_ibiss_6411

Jevremović S, Trajković M, Jeknić Z, Trifunović-Momčilov M, Antonić Reljin D, Marković M, Subotić A, Radojević L. In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants. in Botanica Serbica. 2015;39(2):129-136.
https://hdl.handle.net/21.15107/rcub_ibiss_6411 .

Jevremović, Slađana, Trajković, Milena, Jeknić, Zoran, Trifunović-Momčilov, Milana, Antonić Reljin, Dragana, Marković, Marija, Subotić, Angelina, Radojević, Ljiljana, "In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants" in Botanica Serbica, 39, no. 2 (2015):129-136,
https://hdl.handle.net/21.15107/rcub_ibiss_6411 .

TY  - JOUR
AU  - Trajković, Milena
AU  - Vinterhalter, Branka
AU  - Subotić, Angelina
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6412
AB  - The regenerative potential of in vitro-produced bulblets was investigated in three commercial Oriental lily cultivars (‘Aubade’, ‘Belcanto’ and ‘Solaia’) in relation to two synthetic cytokinins  BA and TDZ and picloram as representative of auxins. Single bulblet leaflets were excised and cultured on MS medium supplemented with either 6-benzyladenine (BA 0-2.0 mg/l), thidiazurone (TDZ 0-2.0 mg/l) or picloram (PIC 0-3.0 mg/l). In all three cultivars and medium combinations explants after 5 weeks regenerated somatic embryos, bulblets and plantlets. While bulblet production was balanced, plantlet and somatic embryogenesis (SE) production were complementary with pronounced SE production at higher plant growth regulator concentrations and plantlet production at lower concentrations. Picloram had a sharp regeneration demarcation with low plantlet production above 0.5mg/l. BA and TDZ produced SE at all concentrations including hormone-free controls. On media with TDZ and BA there was a gradual change from bulblet regeneration at lower to somatic embryogenesis at higher concentrations. For all three cultivars, details of the regeneration process were studied by histological techniques in TDZ-supplemented medium, showing early stage SE regeneration in all samples. Mature, elongated SE stages were missing, indicating early transition of SE into bulblets. The optimal propagation conditions were elaborated for all three lily cultivars.
AB  - Regenerativni potencijal in vitro razmnoženih lukovica istražen je kod tri komercijalne sorte Orijental ljiljana (‘Aubade’, ‘Belcanto’ and ‘Solaia’) u odnosu na dva sintetička citokinina BAP i  TDZ kao i na pikloram regulator rastenja sa auksinskom aktivnošću. Listići sa in vitro lukovica su izolovani i kultivisani na MS podlogama sa 6-benzil aminopurinom (BAP 0-2.0 mg/l), thidiazuronom (TDZ 0-2.0 mg/l) ili pikloramom (PIC 0-3.0 mg/l). Kod sve tri sorte i u svim kombinacijama podloga eksplantati su nakon 5 nedelja regenerisali somatske embrione, lukovičice ili biljke. Dok je produkcija lukovičica bila izbalansirana produkcija biljaka i produkcija ranih somatskih embriona bile su komplementarne sa izrazitom produkcijom SE na višim koncentracijama regulatora rastenja i produkcijom biljaka na nižim koncentracijama. Pikloram je pokazivao jasnu regeneracionu demarkaciju sa niskom produkcijom biljaka na podlogama sa ili iznad 0.5 mg/l. BAP i TDZ su produkovali somatske embrione na svim koncentracijama regulatora rastenja uključujući i podloge bez regulatora rastenja. Podloge sa  TDZ i BAP pokazivale su postepenu promenu od regeneracije lukovica na nižim prema SE na višim koncentracijama. Kod sva tri kultivara proces regeneracije praćen je histološki na podlogama sa TDZ i pokazano je prisustvo direktne SE. Stariji, izduženi stupnjevi SE su bili odsutni što ukazuje na ranu tranziciju somatskih embriona ljiljana u lukovičice. Optimalni uslovi za razmnožavanje razradjeni su i prikazani za sva tri sorte ljiljana.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars
T1  - Različiti regenerativni potencijal kultivara orjentalnog ljiljana (Lilium sp.)
IS  - 2
VL  - 39
SP  - 159
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6412
ER  - 

@article{
author = "Trajković, Milena and Vinterhalter, Branka and Subotić, Angelina and Vinterhalter, Dragan",
year = "2015",
abstract = "The regenerative potential of in vitro-produced bulblets was investigated in three commercial Oriental lily cultivars (‘Aubade’, ‘Belcanto’ and ‘Solaia’) in relation to two synthetic cytokinins  BA and TDZ and picloram as representative of auxins. Single bulblet leaflets were excised and cultured on MS medium supplemented with either 6-benzyladenine (BA 0-2.0 mg/l), thidiazurone (TDZ 0-2.0 mg/l) or picloram (PIC 0-3.0 mg/l). In all three cultivars and medium combinations explants after 5 weeks regenerated somatic embryos, bulblets and plantlets. While bulblet production was balanced, plantlet and somatic embryogenesis (SE) production were complementary with pronounced SE production at higher plant growth regulator concentrations and plantlet production at lower concentrations. Picloram had a sharp regeneration demarcation with low plantlet production above 0.5mg/l. BA and TDZ produced SE at all concentrations including hormone-free controls. On media with TDZ and BA there was a gradual change from bulblet regeneration at lower to somatic embryogenesis at higher concentrations. For all three cultivars, details of the regeneration process were studied by histological techniques in TDZ-supplemented medium, showing early stage SE regeneration in all samples. Mature, elongated SE stages were missing, indicating early transition of SE into bulblets. The optimal propagation conditions were elaborated for all three lily cultivars., Regenerativni potencijal in vitro razmnoženih lukovica istražen je kod tri komercijalne sorte Orijental ljiljana (‘Aubade’, ‘Belcanto’ and ‘Solaia’) u odnosu na dva sintetička citokinina BAP i  TDZ kao i na pikloram regulator rastenja sa auksinskom aktivnošću. Listići sa in vitro lukovica su izolovani i kultivisani na MS podlogama sa 6-benzil aminopurinom (BAP 0-2.0 mg/l), thidiazuronom (TDZ 0-2.0 mg/l) ili pikloramom (PIC 0-3.0 mg/l). Kod sve tri sorte i u svim kombinacijama podloga eksplantati su nakon 5 nedelja regenerisali somatske embrione, lukovičice ili biljke. Dok je produkcija lukovičica bila izbalansirana produkcija biljaka i produkcija ranih somatskih embriona bile su komplementarne sa izrazitom produkcijom SE na višim koncentracijama regulatora rastenja i produkcijom biljaka na nižim koncentracijama. Pikloram je pokazivao jasnu regeneracionu demarkaciju sa niskom produkcijom biljaka na podlogama sa ili iznad 0.5 mg/l. BAP i TDZ su produkovali somatske embrione na svim koncentracijama regulatora rastenja uključujući i podloge bez regulatora rastenja. Podloge sa  TDZ i BAP pokazivale su postepenu promenu od regeneracije lukovica na nižim prema SE na višim koncentracijama. Kod sva tri kultivara proces regeneracije praćen je histološki na podlogama sa TDZ i pokazano je prisustvo direktne SE. Stariji, izduženi stupnjevi SE su bili odsutni što ukazuje na ranu tranziciju somatskih embriona ljiljana u lukovičice. Optimalni uslovi za razmnožavanje razradjeni su i prikazani za sva tri sorte ljiljana.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars, Različiti regenerativni potencijal kultivara orjentalnog ljiljana (Lilium sp.)",
number = "2",
volume = "39",
pages = "159-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6412"
}

Trajković, M., Vinterhalter, B., Subotić, A.,& Vinterhalter, D.. (2015). Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 39(2), 159-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6412

Trajković M, Vinterhalter B, Subotić A, Vinterhalter D. Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars. in Botanica Serbica. 2015;39(2):159-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6412 .

Trajković, Milena, Vinterhalter, Branka, Subotić, Angelina, Vinterhalter, Dragan, "Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars" in Botanica Serbica, 39, no. 2 (2015):159-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6412 .