TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0168165616315735
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84992192143&origin=SingleRecordEmailAlert&txGid=694651161B2E63C5600F6DE538B0ACEC.wsnAw8kcdt7IPYLO0V48gA:3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2525
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
SP  - 14
EP  - 22
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna and Stančić, Ana and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017",
pages = "14-22"
}

Bukara, K., Drvenica, I., Ilić, V., Stančić, A., Mišić, D., Vasić, B., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology, 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić V, Stančić A, Mišić D, Vasić B, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna, Stančić, Ana, Mišić, Danijela, Vasić, Borislav, Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .

TY  - JOUR
AU  - Drvenica, Ivana T.
AU  - Bukara, Katarina M.
AU  - Ilić, Vesna Lj.
AU  - Mišić, Danijela
AU  - Vasić, Borislav Z.
AU  - Gajić, Radoš B.
AU  - Đorđević, Verica B.
AU  - Veljović, Đorđe N.
AU  - Belić, Aleksandar
AU  - Bugarski, Branko M.
PY  - 2016
UR  - http://doi.wiley.com/10.1002/btpr.2304
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2541
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P<0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
IS  - 4
VL  - 32
DO  - 10.1002/btpr.2304
SP  - 1046
EP  - 1055
ER  - 

@article{
author = "Drvenica, Ivana T. and Bukara, Katarina M. and Ilić, Vesna Lj. and Mišić, Danijela and Vasić, Borislav Z. and Gajić, Radoš B. and Đorđević, Verica B. and Veljović, Đorđe N. and Belić, Aleksandar and Bugarski, Branko M.",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P<0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304",
pages = "1046-1055"
}

Drvenica, I. T., Bukara, K. M., Ilić, V. Lj., Mišić, D., Vasić, B. Z., Gajić, R. B., Đorđević, V. B., Veljović, Đ. N., Belić, A.,& Bugarski, B. M.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress, 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica IT, Bukara KM, Ilić VL, Mišić D, Vasić BZ, Gajić RB, Đorđević VB, Veljović ĐN, Belić A, Bugarski BM. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana T., Bukara, Katarina M., Ilić, Vesna Lj., Mišić, Danijela, Vasić, Borislav Z., Gajić, Radoš B., Đorđević, Verica B., Veljović, Đorđe N., Belić, Aleksandar, Bugarski, Branko M., "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .