TY  - BOOK
AU  - Nešković, Mirjana
AU  - Ninković, Slavica
AU  - Miljuš Đukić, Jovanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5028
AB  - U ovoj publikaciji je iznet presek trenutnog znanja o genetički modifikovanim biljkama. Govoreći o biologiji genetičkih modifikacija uz povezivanje sa procesom evolucije živog sveta, autori daju potencijal za jasnije sagledavanje pitanja genetički modifikovanih biljaka i formiranje kritičkog mišljenja o toj temi, kako kod stručne zajednice tako i kod zainteresovane javnosti. Jasna i koncizna prezentacija doprinosi da knjiga bude podjednako interesantna i razumljiva svima.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive
SP  - 1
EP  - 406
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5028
ER  - 

@book{
author = "Nešković, Mirjana and Ninković, Slavica and Miljuš Đukić, Jovanka",
year = "2022",
abstract = "U ovoj publikaciji je iznet presek trenutnog znanja o genetički modifikovanim biljkama. Govoreći o biologiji genetičkih modifikacija uz povezivanje sa procesom evolucije živog sveta, autori daju potencijal za jasnije sagledavanje pitanja genetički modifikovanih biljaka i formiranje kritičkog mišljenja o toj temi, kako kod stručne zajednice tako i kod zainteresovane javnosti. Jasna i koncizna prezentacija doprinosi da knjiga bude podjednako interesantna i razumljiva svima.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive",
pages = "1-406",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5028"
}

Nešković, M., Ninković, S.,& Miljuš Đukić, J.. (2022). Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 1-406.
https://hdl.handle.net/21.15107/rcub_ibiss_5028

Nešković M, Ninković S, Miljuš Đukić J. Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive. 2022;:1-406.
https://hdl.handle.net/21.15107/rcub_ibiss_5028 .

Nešković, Mirjana, Ninković, Slavica, Miljuš Đukić, Jovanka, "Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive" (2022):1-406,
https://hdl.handle.net/21.15107/rcub_ibiss_5028 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Marin, Marija
AU  - Radović, Svetlana
AU  - Ninković, Slavica
PY  - 2018
UR  - http://link.springer.com/10.1007/s00709-018-1250-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3064
AB  - Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.
T2  - Protoplasma
T1  - Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.
DO  - 10.1007/s00709-018-1250-0
ER  - 

@article{
author = "Banjac, Nevena and Stanišić, Mariana and Savić, Jelena and Ćosić, Tatjana and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Marin, Marija and Radović, Svetlana and Ninković, Slavica",
year = "2018",
abstract = "Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.",
journal = "Protoplasma",
title = "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.",
doi = "10.1007/s00709-018-1250-0"
}

Banjac, N., Stanišić, M., Savić, J., Ćosić, T., Stanisavljević, N., Miljuš-Đukić, J., Marin, M., Radović, S.,& Ninković, S.. (2018). Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma.
https://doi.org/10.1007/s00709-018-1250-0

Banjac N, Stanišić M, Savić J, Ćosić T, Stanisavljević N, Miljuš-Đukić J, Marin M, Radović S, Ninković S. Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate.. in Protoplasma. 2018;.
doi:10.1007/s00709-018-1250-0 .

Banjac, Nevena, Stanišić, Mariana, Savić, Jelena, Ćosić, Tatjana, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Marin, Marija, Radović, Svetlana, Ninković, Slavica, "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate." in Protoplasma (2018),
https://doi.org/10.1007/s00709-018-1250-0 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Senćanski, Jelena
AU  - Simonović, Mladen
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2018
UR  - http://link.springer.com/10.1007/s11738-018-2677-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3057
AB  - This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.
T2  - Acta Physiologiae Plantarum
T1  - Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro
IS  - 6
VL  - 40
DO  - 10.1007/s11738-018-2677-9
SP  - 101
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Senćanski, Jelena and Simonović, Mladen and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2018",
abstract = "This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 × 6H2O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.",
journal = "Acta Physiologiae Plantarum",
title = "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro",
number = "6",
volume = "40",
doi = "10.1007/s11738-018-2677-9",
pages = "101"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Senćanski, J., Simonović, M., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2018). Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum, 40(6), 101.
https://doi.org/10.1007/s11738-018-2677-9

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Senćanski J, Simonović M, Radović S, Vinterhalter D, Vinterhalter B. Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum. 2018;40(6):101.
doi:10.1007/s11738-018-2677-9 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Senćanski, Jelena, Simonović, Mladen, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro" in Acta Physiologiae Plantarum, 40, no. 6 (2018):101,
https://doi.org/10.1007/s11738-018-2677-9 . .

TY  - GEN
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
AU  - Miljuš-Đukić, Jovanka D.
AU  - Jovanović, Z.
AU  - Orbović, V.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1949
AB  - Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation
   with the blue light of plantlets at dawn resulted in moderate PT
   response regarding both attained curvature and long lag phase. The PT
   response was the strongest between 8:00 and 12:00 h. Throughout the
   afternoon and in the evening, bending rate and maximal PT curvature
   declined significantly until 23:00 h. The GT response was fastest and
   strongest for plantlets stimulated early in the morning and late in the
   evening. During the rest of the day, GT competence did not change much
   apart from a minimum at 15:00. In conditions comprising either prolonged
   day or prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in a short-term. Introduction of dark
   period prior to the tropic stimulation at 11:00 h when both PT and GT
   responses were strong resulted in the opposite effect: PT was depressed
   and GT was enhanced. There was a time threshold of 60 min for the
   duration of dark period so the plants can sense interruption in the
   daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.
T2  - Journal of Plant Growth Regulation
T1  - Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)
IS  - 2
VL  - 34
DO  - 10.1007/s00344-015-9507-8
SP  - 440
EP  - 450
ER  - 

@misc{
author = "Vinterhalter, Dragan and Vinterhalter, Branka and Miljuš-Đukić, Jovanka D. and Jovanović, Z. and Orbović, V.",
year = "2015",
abstract = "Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation
   with the blue light of plantlets at dawn resulted in moderate PT
   response regarding both attained curvature and long lag phase. The PT
   response was the strongest between 8:00 and 12:00 h. Throughout the
   afternoon and in the evening, bending rate and maximal PT curvature
   declined significantly until 23:00 h. The GT response was fastest and
   strongest for plantlets stimulated early in the morning and late in the
   evening. During the rest of the day, GT competence did not change much
   apart from a minimum at 15:00. In conditions comprising either prolonged
   day or prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in a short-term. Introduction of dark
   period prior to the tropic stimulation at 11:00 h when both PT and GT
   responses were strong resulted in the opposite effect: PT was depressed
   and GT was enhanced. There was a time threshold of 60 min for the
   duration of dark period so the plants can sense interruption in the
   daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.",
journal = "Journal of Plant Growth Regulation",
title = "Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)",
number = "2",
volume = "34",
doi = "10.1007/s00344-015-9507-8",
pages = "440-450"
}

Vinterhalter, D., Vinterhalter, B., Miljuš-Đukić, J. D., Jovanović, Z.,& Orbović, V.. (2015). Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014). in Journal of Plant Growth Regulation, 34(2), 440-450.
https://doi.org/10.1007/s00344-015-9507-8

Vinterhalter D, Vinterhalter B, Miljuš-Đukić JD, Jovanović Z, Orbović V. Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014). in Journal of Plant Growth Regulation. 2015;34(2):440-450.
doi:10.1007/s00344-015-9507-8 .

Vinterhalter, Dragan, Vinterhalter, Branka, Miljuš-Đukić, Jovanka D., Jovanović, Z., Orbović, V., "Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets (vol 33, pg 539, 2014)" in Journal of Plant Growth Regulation, 34, no. 2 (2015):440-450,
https://doi.org/10.1007/s00344-015-9507-8 . .

TY  - CONF
AU  - Banović, Bojana
AU  - Dinić, Svetlana
AU  - Vidaković, Melita
AU  - Miljuš Đukić, Jovanka
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6317
AB  - Common buckwheat (Fagopyrum esculentum Moench) is a self-incompatible dicot species with two morphologically different flower types: thrum (shorter style, longer anthers, larger pollen grains) and pin (longer style, shorter anthers, smaller pollen grains). In this species fertilization is allowed only between flowers of different morphology (compatible pollination), while it is prevented between flowers of the same morphology (incompatible pollination) through self-incompatibility (SI). Two flower morphs have different expression site of the SI response: in thrum pistil at the junction of stigma and style, and in pin pistil at 2/3 of style’s length. It is well documented that SI response includes various protein-protein interactions in other studied plant SI species, but so far there are no such reports for buckwheat. The aim of our study was to track and compare changes in proteome profiles of thrum and pin pistils’ upon their incompatible and compatible pollination. Total proteins extracted from non-pollinated, incompatibly and compatibly pollinated thrum and pin pistils were separated by 2D-PAGE and analyzed using Image Master 2D Platinum software v6.0. In each sample, proteome profiles revealed distribution over a wide mass range (10-130 kDa), with the prevalence of acidic to neutral proteins (pI 3.5-7) and only a few basic proteins (pI 7.5-9). The most abundant proteins were those shared by two morphs, which were present independently of pollination type (incompatible/compatible) (c. 90-95% of detected spots per sample). Proteins specific for morph and pollination type were also discovered (c. 5-10% of detected spots per sample) and will be identified by MS analysis.
PB  - International Association of Sexua Plant Reproduction Research
C3  - 23rd International Congress on Sexual Plant Reproduction: Seeds for the future; 2014 Jul 13-18; Porto, Portugal
T1  - Comparative 2D-proteome analyses of non-pollinated, incompatibly and compatibly pollinated thrum and pin pistils of common buckwheat
SP  - 148
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6317
ER  - 

@conference{
author = "Banović, Bojana and Dinić, Svetlana and Vidaković, Melita and Miljuš Đukić, Jovanka",
year = "2014",
abstract = "Common buckwheat (Fagopyrum esculentum Moench) is a self-incompatible dicot species with two morphologically different flower types: thrum (shorter style, longer anthers, larger pollen grains) and pin (longer style, shorter anthers, smaller pollen grains). In this species fertilization is allowed only between flowers of different morphology (compatible pollination), while it is prevented between flowers of the same morphology (incompatible pollination) through self-incompatibility (SI). Two flower morphs have different expression site of the SI response: in thrum pistil at the junction of stigma and style, and in pin pistil at 2/3 of style’s length. It is well documented that SI response includes various protein-protein interactions in other studied plant SI species, but so far there are no such reports for buckwheat. The aim of our study was to track and compare changes in proteome profiles of thrum and pin pistils’ upon their incompatible and compatible pollination. Total proteins extracted from non-pollinated, incompatibly and compatibly pollinated thrum and pin pistils were separated by 2D-PAGE and analyzed using Image Master 2D Platinum software v6.0. In each sample, proteome profiles revealed distribution over a wide mass range (10-130 kDa), with the prevalence of acidic to neutral proteins (pI 3.5-7) and only a few basic proteins (pI 7.5-9). The most abundant proteins were those shared by two morphs, which were present independently of pollination type (incompatible/compatible) (c. 90-95% of detected spots per sample). Proteins specific for morph and pollination type were also discovered (c. 5-10% of detected spots per sample) and will be identified by MS analysis.",
publisher = "International Association of Sexua Plant Reproduction Research",
journal = "23rd International Congress on Sexual Plant Reproduction: Seeds for the future; 2014 Jul 13-18; Porto, Portugal",
title = "Comparative 2D-proteome analyses of non-pollinated, incompatibly and compatibly pollinated thrum and pin pistils of common buckwheat",
pages = "148-148",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6317"
}

Banović, B., Dinić, S., Vidaković, M.,& Miljuš Đukić, J.. (2014). Comparative 2D-proteome analyses of non-pollinated, incompatibly and compatibly pollinated thrum and pin pistils of common buckwheat. in 23rd International Congress on Sexual Plant Reproduction: Seeds for the future; 2014 Jul 13-18; Porto, Portugal
International Association of Sexua Plant Reproduction Research., 148-148.
https://hdl.handle.net/21.15107/rcub_ibiss_6317

Banović B, Dinić S, Vidaković M, Miljuš Đukić J. Comparative 2D-proteome analyses of non-pollinated, incompatibly and compatibly pollinated thrum and pin pistils of common buckwheat. in 23rd International Congress on Sexual Plant Reproduction: Seeds for the future; 2014 Jul 13-18; Porto, Portugal. 2014;:148-148.
https://hdl.handle.net/21.15107/rcub_ibiss_6317 .

Banović, Bojana, Dinić, Svetlana, Vidaković, Melita, Miljuš Đukić, Jovanka, "Comparative 2D-proteome analyses of non-pollinated, incompatibly and compatibly pollinated thrum and pin pistils of common buckwheat" in 23rd International Congress on Sexual Plant Reproduction: Seeds for the future; 2014 Jul 13-18; Porto, Portugal (2014):148-148,
https://hdl.handle.net/21.15107/rcub_ibiss_6317 .

TY  - JOUR
AU  - Vinterhalter, D.
AU  - Vinterhalter, Branka
AU  - Miljuš-Đukić, Jovanka D.
AU  - Jovanovic, Z.
AU  - Orbovic, V.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2163
AB  - Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation of
   plantlets with blue light at dawn resulted in moderate PT response
   regarding both attained curvature and long lag phase. The PT response
   was the strongest between 8:00 and 12:00 h. Throughout the afternoon and
   in the evening, bending rate and maximal PT curvature declined
   significantly until 23:00 h. The GT response was fastest and strongest
   for plantlets stimulated early in the morning and late in the evening.
   During the rest of the day, GT competence did not change much apart from
   a minimum at 15:00. In conditions comprising either prolonged day or
   prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in the short-term. Introduction of a
   dark period prior to the tropic stimulation at 11:00 h when both PT and
   GT responses were strong resulted in the opposite effect: PT was
   depressed, and GT was enhanced. There was a time threshold of 60 min for
   the duration of the dark period so the plants can sense interruption in
   the daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. The PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached a daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.
T2  - Journal of Plant Growth Regulation
T1  - Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets
IS  - 3
VL  - 33
DO  - 10.1007/s00344-013-9403-z
SP  - 539
EP  - 550
ER  - 

@article{
author = "Vinterhalter, D. and Vinterhalter, Branka and Miljuš-Đukić, Jovanka D. and Jovanovic, Z. and Orbovic, V.",
year = "2014",
abstract = "Competence for phototropic (PT) and gravitropic (GT) bending by potato
   plantlets grown in vitro manifests regular daily changes indicating
   possible involvement of circadian regulation. Unilateral stimulation of
   plantlets with blue light at dawn resulted in moderate PT response
   regarding both attained curvature and long lag phase. The PT response
   was the strongest between 8:00 and 12:00 h. Throughout the afternoon and
   in the evening, bending rate and maximal PT curvature declined
   significantly until 23:00 h. The GT response was fastest and strongest
   for plantlets stimulated early in the morning and late in the evening.
   During the rest of the day, GT competence did not change much apart from
   a minimum at 15:00. In conditions comprising either prolonged day or
   prolonged night, plantlets appeared to maintain rhythmicity of
   competence for PT and GT at least in the short-term. Introduction of a
   dark period prior to the tropic stimulation at 11:00 h when both PT and
   GT responses were strong resulted in the opposite effect: PT was
   depressed, and GT was enhanced. There was a time threshold of 60 min for
   the duration of the dark period so the plants can sense interruption in
   the daylight. Levels of relative expression of a PHOT2 gene indicate
   rhythmic daily changes. The PHOT2 gene was present at high levels during
   morning hours and late in the evening. As the mid-day and the afternoon
   hours approached, PHOT2 expression decreased and reached a daily minimum
   at 17:00 h. We believe that our data offer strong support for the
   conclusion that there is an involvement of circadian rhythms in control
   of both PT and GT.",
journal = "Journal of Plant Growth Regulation",
title = "Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets",
number = "3",
volume = "33",
doi = "10.1007/s00344-013-9403-z",
pages = "539-550"
}

Vinterhalter, D., Vinterhalter, B., Miljuš-Đukić, J. D., Jovanovic, Z.,& Orbovic, V.. (2014). Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets. in Journal of Plant Growth Regulation, 33(3), 539-550.
https://doi.org/10.1007/s00344-013-9403-z

Vinterhalter D, Vinterhalter B, Miljuš-Đukić JD, Jovanovic Z, Orbovic V. Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets. in Journal of Plant Growth Regulation. 2014;33(3):539-550.
doi:10.1007/s00344-013-9403-z .

Vinterhalter, D., Vinterhalter, Branka, Miljuš-Đukić, Jovanka D., Jovanovic, Z., Orbovic, V., "Daily Changes in the Competence for Photo- and Gravitropic Response by
 Potato Plantlets" in Journal of Plant Growth Regulation, 33, no. 3 (2014):539-550,
https://doi.org/10.1007/s00344-013-9403-z . .

TY  - JOUR
AU  - Nikolić, Radomirka R
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
AU  - Dragićević, Milan
AU  - Miljuš-Đukić, Jovanka D.
AU  - Banović, Bojana
AU  - Bohanec, Borut
AU  - Savić, Jelena
AU  - Banjac, Nevena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/957
AB  - Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.
T2  - Annals of Applied Biology
T1  - Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin
IS  - 3
VL  - 163
DO  - 10.1111/aab.12071
SP  - 247
EP  - 493
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_957
ER  - 

@article{
author = "Nikolić, Radomirka R and Zdravković-Korać, Snežana and Ninković, Slavica and Dragićević, Milan and Miljuš-Đukić, Jovanka D. and Banović, Bojana and Bohanec, Borut and Savić, Jelena and Banjac, Nevena",
year = "2013",
abstract = "Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.",
journal = "Annals of Applied Biology",
title = "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin",
number = "3",
volume = "163",
doi = "10.1111/aab.12071",
pages = "247-493",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_957"
}

Nikolić, R. R., Zdravković-Korać, S., Ninković, S., Dragićević, M., Miljuš-Đukić, J. D., Banović, B., Bohanec, B., Savić, J.,& Banjac, N.. (2013). Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology, 163(3), 247-493.
https://doi.org/10.1111/aab.12071
https://hdl.handle.net/21.15107/rcub_ibiss_957

Nikolić RR, Zdravković-Korać S, Ninković S, Dragićević M, Miljuš-Đukić JD, Banović B, Bohanec B, Savić J, Banjac N. Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology. 2013;163(3):247-493.
doi:10.1111/aab.12071
https://hdl.handle.net/21.15107/rcub_ibiss_957 .

Nikolić, Radomirka R, Zdravković-Korać, Snežana, Ninković, Slavica, Dragićević, Milan, Miljuš-Đukić, Jovanka D., Banović, Bojana, Bohanec, Borut, Savić, Jelena, Banjac, Nevena, "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin" in Annals of Applied Biology, 163, no. 3 (2013):247-493,
https://doi.org/10.1111/aab.12071 .,
https://hdl.handle.net/21.15107/rcub_ibiss_957 .

TY  - JOUR
AU  - Stanisavljević, Nemanja S
AU  - Savić, Jelena M.
AU  - Jovanović, Zivko S
AU  - Miljuš-Đukić, Jovanka D.
AU  - Radović, Svetlana R
AU  - Vinterhalter, Dragan V
AU  - Vinterhalter, Branka
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1142
AB  - Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.
T2  - Acta Physiologiae Plantarum
T1  - Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level
IS  - 5
VL  - 34
SP  - 339
EP  - 2006
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1142
ER  - 

@article{
author = "Stanisavljević, Nemanja S and Savić, Jelena M. and Jovanović, Zivko S and Miljuš-Đukić, Jovanka D. and Radović, Svetlana R and Vinterhalter, Dragan V and Vinterhalter, Branka",
year = "2012",
abstract = "Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.",
journal = "Acta Physiologiae Plantarum",
title = "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level",
number = "5",
volume = "34",
pages = "339-2006",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1142"
}

Stanisavljević, N. S., Savić, J. M., Jovanović, Z. S., Miljuš-Đukić, J. D., Radović, S. R., Vinterhalter, D. V.,& Vinterhalter, B.. (2012). Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum, 34(5), 339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142

Stanisavljević NS, Savić JM, Jovanović ZS, Miljuš-Đukić JD, Radović SR, Vinterhalter DV, Vinterhalter B. Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum. 2012;34(5):339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

Stanisavljević, Nemanja S, Savić, Jelena M., Jovanović, Zivko S, Miljuš-Đukić, Jovanka D., Radović, Svetlana R, Vinterhalter, Dragan V, Vinterhalter, Branka, "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level" in Acta Physiologiae Plantarum, 34, no. 5 (2012):339-2006,
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Miljuš-Đukić, Jovanka
AU  - Vinterhalter, Dragan
PY  - 2011
UR  - https://bp.ueb.cas.cz/pdfs/bpl/2011/03/02.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6167
AB  - Differences among three clones of Gentiana punctata L. hairy root shoot regenerants were investigated in relation to their growth patterns, production of secondary metabolites and 2D protein profiles. Prominent differences in growth parameters were stable thus qualifying regenerant clones as true somaclones. Marked differences in protein spots were registered among the regenerant clones but not in comparison with the non-transformed control. Southern blot hybridization of regenerants showed the absence of rolA, B and C genes, initially present in the main hairy root lines. Orf13 and rolD were present and orf8 was missing in all three regenerant clones whereas orf3 was missing only in clone 2. Although lacking the three major rol genes, plants of regenerant clones retained characteristics of the hairy root phenotype.
PB  - Dordrecht: Springer-Verlag
T2  - Biologia Plantarum
T1  - Variability in shoot cultures of hairy root regenerated Gentiana punctata L.
IS  - 3
VL  - 55
DO  - 10.1007/s10535-011-0105-8
SP  - 414
EP  - 422
ER  - 

@article{
author = "Vinterhalter, Branka and Zdravković-Korać, Snežana and Ninković, Slavica and Banjac, Nevena and Janković, Teodora and Miljuš-Đukić, Jovanka and Vinterhalter, Dragan",
year = "2011",
abstract = "Differences among three clones of Gentiana punctata L. hairy root shoot regenerants were investigated in relation to their growth patterns, production of secondary metabolites and 2D protein profiles. Prominent differences in growth parameters were stable thus qualifying regenerant clones as true somaclones. Marked differences in protein spots were registered among the regenerant clones but not in comparison with the non-transformed control. Southern blot hybridization of regenerants showed the absence of rolA, B and C genes, initially present in the main hairy root lines. Orf13 and rolD were present and orf8 was missing in all three regenerant clones whereas orf3 was missing only in clone 2. Although lacking the three major rol genes, plants of regenerant clones retained characteristics of the hairy root phenotype.",
publisher = "Dordrecht: Springer-Verlag",
journal = "Biologia Plantarum",
title = "Variability in shoot cultures of hairy root regenerated Gentiana punctata L.",
number = "3",
volume = "55",
doi = "10.1007/s10535-011-0105-8",
pages = "414-422"
}

Vinterhalter, B., Zdravković-Korać, S., Ninković, S., Banjac, N., Janković, T., Miljuš-Đukić, J.,& Vinterhalter, D.. (2011). Variability in shoot cultures of hairy root regenerated Gentiana punctata L.. in Biologia Plantarum
Dordrecht: Springer-Verlag., 55(3), 414-422.
https://doi.org/10.1007/s10535-011-0105-8

Vinterhalter B, Zdravković-Korać S, Ninković S, Banjac N, Janković T, Miljuš-Đukić J, Vinterhalter D. Variability in shoot cultures of hairy root regenerated Gentiana punctata L.. in Biologia Plantarum. 2011;55(3):414-422.
doi:10.1007/s10535-011-0105-8 .

Vinterhalter, Branka, Zdravković-Korać, Snežana, Ninković, Slavica, Banjac, Nevena, Janković, Teodora, Miljuš-Đukić, Jovanka, Vinterhalter, Dragan, "Variability in shoot cultures of hairy root regenerated Gentiana punctata L." in Biologia Plantarum, 55, no. 3 (2011):414-422,
https://doi.org/10.1007/s10535-011-0105-8 . .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana D.
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1624
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.
T2  - Biologia Plantarum
T1  - Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata
IS  - 4
VL  - 50
EP  - 530
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1624
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana D. and Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Vinterhalter, Branka and Vinterhalter, Dragan V",
year = "2006",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.",
journal = "Biologia Plantarum",
title = "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata",
number = "4",
volume = "50",
pages = "530",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1624"
}

Sretenović-Rajičić, T. D., Ninković, S., Miljuš-Đukić, J. D., Vinterhalter, B.,& Vinterhalter, D. V.. (2006). Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1624

Sretenović-Rajičić TD, Ninković S, Miljuš-Đukić JD, Vinterhalter B, Vinterhalter DV. Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum. 2006;50(4):null-530.
https://hdl.handle.net/21.15107/rcub_ibiss_1624 .

Sretenović-Rajičić, Tatjana D., Ninković, Slavica, Miljuš-Đukić, Jovanka D., Vinterhalter, Branka, Vinterhalter, Dragan V, "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1624 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Nikolić, Radomirka R
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Nešković, Mirjana
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1747
AB  - The use of two Agrobacterium tumefaciens strains for transformation of Triticum aestivum L. cv. Vesna was studied. Immature embryos, isolated 15 d after pollination, were co-cultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T-0 plants, as well as the T-1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible.
T2  - Biologia Plantarum
T1  - Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.
IS  - 2
VL  - 48
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1747
ER  - 

@article{
author = "Banjac, Nevena and Nikolić, Radomirka R and Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Nešković, Mirjana",
year = "2004",
abstract = "The use of two Agrobacterium tumefaciens strains for transformation of Triticum aestivum L. cv. Vesna was studied. Immature embryos, isolated 15 d after pollination, were co-cultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T-0 plants, as well as the T-1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible.",
journal = "Biologia Plantarum",
title = "Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.",
number = "2",
volume = "48",
pages = "184",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1747"
}

Banjac, N., Nikolić, R. R., Ninković, S., Miljuš-Đukić, J. D.,& Nešković, M.. (2004). Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.. in Biologia Plantarum, 48(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1747

Banjac N, Nikolić RR, Ninković S, Miljuš-Đukić JD, Nešković M. Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.. in Biologia Plantarum. 2004;48(2):null-184.
https://hdl.handle.net/21.15107/rcub_ibiss_1747 .

Banjac, Nevena, Nikolić, Radomirka R, Ninković, Slavica, Miljuš-Đukić, Jovanka D., Nešković, Mirjana, "Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L." in Biologia Plantarum, 48, no. 2 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1747 .

TY  - JOUR
AU  - Bijelović, Aneta
AU  - Rosić, Nedeljka
AU  - Miljuš-Đukić, Jovanka D.
AU  - Ninković, Slavica
AU  - Grubišić, Dragoljub
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1749
AB  - In vitro root culture of yellow wort (Blackstonia perfoliata ( L.) Huds.) was initiated on Murashige and Skoog ( MS) medium. In the presence of benzylaminopurine ( BAP) numerous adventitious buds formed, which developed into shoots. Presence of indole-3-butyric acid ( IBA) in media significantly decreased number of buds, but increased development of lateral roots. On hormone-free medium shoots successfully rooted and developed flowers and viable seeds that formed another generation. Shoot cultures of B. perfoliata inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS developed hairy roots at 3 weeks and they were cultured on hormone-free MS medium. Spontaneous shoot regeneration occurred in 3 clones.
T2  - Biologia Plantarum
T1  - In vitro regeneration and transformation of Blackstonia perfoliata
IS  - 3
VL  - 48
EP  - 338
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1749
ER  - 

@article{
author = "Bijelović, Aneta and Rosić, Nedeljka and Miljuš-Đukić, Jovanka D. and Ninković, Slavica and Grubišić, Dragoljub",
year = "2004",
abstract = "In vitro root culture of yellow wort (Blackstonia perfoliata ( L.) Huds.) was initiated on Murashige and Skoog ( MS) medium. In the presence of benzylaminopurine ( BAP) numerous adventitious buds formed, which developed into shoots. Presence of indole-3-butyric acid ( IBA) in media significantly decreased number of buds, but increased development of lateral roots. On hormone-free medium shoots successfully rooted and developed flowers and viable seeds that formed another generation. Shoot cultures of B. perfoliata inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS developed hairy roots at 3 weeks and they were cultured on hormone-free MS medium. Spontaneous shoot regeneration occurred in 3 clones.",
journal = "Biologia Plantarum",
title = "In vitro regeneration and transformation of Blackstonia perfoliata",
number = "3",
volume = "48",
pages = "338",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1749"
}

Bijelović, A., Rosić, N., Miljuš-Đukić, J. D., Ninković, S.,& Grubišić, D.. (2004). In vitro regeneration and transformation of Blackstonia perfoliata. in Biologia Plantarum, 48(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1749

Bijelović A, Rosić N, Miljuš-Đukić JD, Ninković S, Grubišić D. In vitro regeneration and transformation of Blackstonia perfoliata. in Biologia Plantarum. 2004;48(3):null-338.
https://hdl.handle.net/21.15107/rcub_ibiss_1749 .

Bijelović, Aneta, Rosić, Nedeljka, Miljuš-Đukić, Jovanka D., Ninković, Slavica, Grubišić, Dragoljub, "In vitro regeneration and transformation of Blackstonia perfoliata" in Biologia Plantarum, 48, no. 3 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1749 .

TY  - JOUR
AU  - Miljuš-Đukić, Jovanka D.
AU  - Ninković, Slavica
AU  - Radović, Svetlana R
AU  - Maksimović, Vesna R
AU  - Brkljačić, Jelena M.
AU  - Nešković, Mirjana
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1748
AB  - Buckwheat ( Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.
T2  - Biologia Plantarum
T1  - Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat
IS  - 2
VL  - 48
EP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1748
ER  - 

@article{
author = "Miljuš-Đukić, Jovanka D. and Ninković, Slavica and Radović, Svetlana R and Maksimović, Vesna R and Brkljačić, Jelena M. and Nešković, Mirjana",
year = "2004",
abstract = "Buckwheat ( Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.",
journal = "Biologia Plantarum",
title = "Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat",
number = "2",
volume = "48",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1748"
}

Miljuš-Đukić, J. D., Ninković, S., Radović, S. R., Maksimović, V. R., Brkljačić, J. M.,& Nešković, M.. (2004). Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat. in Biologia Plantarum, 48(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1748

Miljuš-Đukić JD, Ninković S, Radović SR, Maksimović VR, Brkljačić JM, Nešković M. Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat. in Biologia Plantarum. 2004;48(2):null-296.
https://hdl.handle.net/21.15107/rcub_ibiss_1748 .

Miljuš-Đukić, Jovanka D., Ninković, Slavica, Radović, Svetlana R, Maksimović, Vesna R, Brkljačić, Jelena M., Nešković, Mirjana, "Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat" in Biologia Plantarum, 48, no. 2 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1748 .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Lazarević, Jelica
PY  - 2001
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/426
AB  - Proteinase inhibitors (PI) are defense proteins in plants. In interaction with other chemicals, they determine plant resistance to herbivorous insects. Regarding the fact that their target sites are proteinases, central control elements in insect development, producing transgenic plants with PI genes could be an efficient pest management strategy. There are numerous examples of successfully transformed plants. However, the level of control have not justified their commercial use as many insects can quickly adapt to the presence of PI in food. Factors that must be taken into consideration in selection of appropriate inhibitor are reviewed in this paper. It is important to know: (1)specificity and binding capacity of PI as well as its sensitivity to plant proteinases, (2)regulatory mechanisms of the expression of genes determining proteinases in insects and PI in plants (3)genetic variability of resistance and tolerance in plant and insect populations and (4)effects of PI on predators and parasites of herbivorous insects as well as other useful insects such as pollinators.
T2  - Acta biologica iugoslavica - serija D: Ekologija
T1  - Proteinazni inhibitori i mogućnost njihove primene u kontroli štetnih insekata
T1  - Proteinase inhibitors and the potential of their application in insect pest management
IS  - 1
VL  - 36
SP  - 1
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_426
ER  - 

@article{
author = "Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Lazarević, Jelica",
year = "2001, 2001",
abstract = "Proteinase inhibitors (PI) are defense proteins in plants. In interaction with other chemicals, they determine plant resistance to herbivorous insects. Regarding the fact that their target sites are proteinases, central control elements in insect development, producing transgenic plants with PI genes could be an efficient pest management strategy. There are numerous examples of successfully transformed plants. However, the level of control have not justified their commercial use as many insects can quickly adapt to the presence of PI in food. Factors that must be taken into consideration in selection of appropriate inhibitor are reviewed in this paper. It is important to know: (1)specificity and binding capacity of PI as well as its sensitivity to plant proteinases, (2)regulatory mechanisms of the expression of genes determining proteinases in insects and PI in plants (3)genetic variability of resistance and tolerance in plant and insect populations and (4)effects of PI on predators and parasites of herbivorous insects as well as other useful insects such as pollinators.",
journal = "Acta biologica iugoslavica - serija D: Ekologija",
title = "Proteinazni inhibitori i mogućnost njihove primene u kontroli štetnih insekata, Proteinase inhibitors and the potential of their application in insect pest management",
number = "1",
volume = "36",
pages = "1-38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_426"
}

Ninković, S., Miljuš-Đukić, J. D.,& Lazarević, J.. (2001). Proteinazni inhibitori i mogućnost njihove primene u kontroli štetnih insekata. in Acta biologica iugoslavica - serija D: Ekologija, 36(1), 1-38.
https://hdl.handle.net/21.15107/rcub_ibiss_426

Ninković S, Miljuš-Đukić JD, Lazarević J. Proteinazni inhibitori i mogućnost njihove primene u kontroli štetnih insekata. in Acta biologica iugoslavica - serija D: Ekologija. 2001;36(1):1-38.
https://hdl.handle.net/21.15107/rcub_ibiss_426 .

Ninković, Slavica, Miljuš-Đukić, Jovanka D., Lazarević, Jelica, "Proteinazni inhibitori i mogućnost njihove primene u kontroli štetnih insekata" in Acta biologica iugoslavica - serija D: Ekologija, 36, no. 1 (2001):1-38,
https://hdl.handle.net/21.15107/rcub_ibiss_426 .