TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6466
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
IS  - 7
VL  - 12
DO  - 10.3390/antiox12071424
SP  - 1424
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš and Tešić, Živoslav and Stanojević, Sladjana and Pešić, Mirjana",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
number = "7",
volume = "12",
doi = "10.3390/antiox12071424",
pages = "1424"
}

Kostić, A., Milinčić, D., Špirović Trifunović, B., Nedić, N., Gašić, U., Tešić, Ž., Stanojević, S.,& Pešić, M.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants
Basel: MDPI., 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić A, Milinčić D, Špirović Trifunović B, Nedić N, Gašić U, Tešić Ž, Stanojević S, Pešić M. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar, Milinčić, Danijel, Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš, Tešić, Živoslav, Stanojević, Sladjana, Pešić, Mirjana, "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6467
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
SP  - 477
ER  - 

@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477",
pages = "477"
}

Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae
Basel: MDPI., 9(4), 477.
https://doi.org/10.3390/horticulturae9040477

Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4):477.
doi:10.3390/horticulturae9040477 .

Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023):477,
https://doi.org/10.3390/horticulturae9040477 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Mačukanović-Jocić, Marina
AU  - Radošević, Radenko
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6306
AB  - The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040424
SP  - 424
ER  - 

@article{
author = "Fotirić Akšić, Milica and Mačukanović-Jocić, Marina and Radošević, Radenko and Nedić, Nebojša and Gašić, Uroš and Tosti, Tomislav and Tešić, Živoslav and Meland, Mekjell",
year = "2023",
abstract = "The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040424",
pages = "424"
}

Fotirić Akšić, M., Mačukanović-Jocić, M., Radošević, R., Nedić, N., Gašić, U., Tosti, T., Tešić, Ž.,& Meland, M.. (2023). The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae
Basel: MDPI., 9(4), 424.
https://doi.org/10.3390/horticulturae9040424

Fotirić Akšić M, Mačukanović-Jocić M, Radošević R, Nedić N, Gašić U, Tosti T, Tešić Ž, Meland M. The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae. 2023;9(4):424.
doi:10.3390/horticulturae9040424 .

Fotirić Akšić, Milica, Mačukanović-Jocić, Marina, Radošević, Radenko, Nedić, Nebojša, Gašić, Uroš, Tosti, Tomislav, Tešić, Živoslav, Meland, Mekjell, "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora" in Horticulturae, 9, no. 4 (2023):424,
https://doi.org/10.3390/horticulturae9040424 . .

TY  - JOUR
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš
AU  - Đurović, Dejan
AU  - Tešić, Živoslav
AU  - Fotirić Akšić, Milica
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9653822
UR  - https://www.mdpi.com/1420-3049/27/21/7651
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5239
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - Basel: MDPI
T2  - Molecules
T1  - Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia
IS  - 21
VL  - 27
DO  - 10.3390/molecules27217651
SP  - 7651
ER  - 

@article{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš and Đurović, Dejan and Tešić, Živoslav and Fotirić Akšić, Milica and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia",
number = "21",
volume = "27",
doi = "10.3390/molecules27217651",
pages = "7651"
}

Horvacki, N., Andrić, F., Gašić, U., Đurović, D., Tešić, Ž., Fotirić Akšić, M.,& Milojković-Opsenica, D.. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules
Basel: MDPI., 27(21), 7651.
https://doi.org/10.3390/molecules27217651

Horvacki N, Andrić F, Gašić U, Đurović D, Tešić Ž, Fotirić Akšić M, Milojković-Opsenica D. Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules. 2022;27(21):7651.
doi:10.3390/molecules27217651 .

Horvacki, Nikola, Andrić, Filip, Gašić, Uroš, Đurović, Dejan, Tešić, Živoslav, Fotirić Akšić, Milica, Milojković-Opsenica, Dušanka, "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia" in Molecules, 27, no. 21 (2022):7651,
https://doi.org/10.3390/molecules27217651 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Nešović, Milica
AU  - Ćirić, Ivanka
AU  - Tešić, Živoslav
AU  - Pezo, Lato
AU  - Tosti, Tomislav
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Lončar, Biljana
AU  - Meland, Mekjell
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/9/765
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5130
AB  - Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions
IS  - 9
VL  - 8
DO  - 10.3390/horticulturae8090765
SP  - 765
ER  - 

@article{
author = "Fotirić Akšić, Milica and Nešović, Milica and Ćirić, Ivanka and Tešić, Živoslav and Pezo, Lato and Tosti, Tomislav and Gašić, Uroš and Dojčinović, Biljana and Lončar, Biljana and Meland, Mekjell",
year = "2022",
abstract = "Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions",
number = "9",
volume = "8",
doi = "10.3390/horticulturae8090765",
pages = "765"
}

Fotirić Akšić, M., Nešović, M., Ćirić, I., Tešić, Ž., Pezo, L., Tosti, T., Gašić, U., Dojčinović, B., Lončar, B.,& Meland, M.. (2022). Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae
Basel: MDPI., 8(9), 765.
https://doi.org/10.3390/horticulturae8090765

Fotirić Akšić M, Nešović M, Ćirić I, Tešić Ž, Pezo L, Tosti T, Gašić U, Dojčinović B, Lončar B, Meland M. Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae. 2022;8(9):765.
doi:10.3390/horticulturae8090765 .

Fotirić Akšić, Milica, Nešović, Milica, Ćirić, Ivanka, Tešić, Živoslav, Pezo, Lato, Tosti, Tomislav, Gašić, Uroš, Dojčinović, Biljana, Lončar, Biljana, Meland, Mekjell, "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions" in Horticulturae, 8, no. 9 (2022):765,
https://doi.org/10.3390/horticulturae8090765 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Nešović, Milica
AU  - Ćirić, Ivanka
AU  - Tešić, Živoslav
AU  - Pezo, Lato
AU  - Tosti, Tomislav
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Lončar, Biljana
AU  - Meland, Mekjell
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.941487/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5081
AB  - Using modern analytical techniques, a comprehensive study of the chemical composition of fruits from apple cultivars grown in Western Norway during 2019 and 2020 was done. Metals, sugars, organic acids, antioxidant tests, and polyphenol content have been observed. In all investigated samples, the most dominant sugars were glucose, fructose, and sucrose. Among 11 tested organic acids, the dominant was malic acid, followed by citric and maleic acid. The most common metal was potassium, followed by magnesium and zinc. The quantification of polyphenols showed that among the 11 quantified polyphenols, chlorogenic acid, quercetin 3- O- rhamnoside, quercetin 3 -O -glucoside, quercetin, and phlorizin were the most abundant. A detailed study of the polyphenolic profile of nine investigated apple samples provided 30 identified polyphenolic compounds from the class of hydroxybenzoic and hydroxycinnamic acids, flavonoids, and dihydrochalcones. In addition to the identified 3- O -caffeoylquinic acid, its two isomers of 5- O -caffeoylquinic acid and three esters were also found. Present polyphenols of the tested apples provided significant data on the quality of Norwegian apples, and they contribute to the distinguishing of these apple samples.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars
VL  - 9
DO  - 10.3389/fnut.2022.941487
SP  - 941487
ER  - 

@article{
author = "Fotirić Akšić, Milica and Nešović, Milica and Ćirić, Ivanka and Tešić, Živoslav and Pezo, Lato and Tosti, Tomislav and Gašić, Uroš and Dojčinović, Biljana and Lončar, Biljana and Meland, Mekjell",
year = "2022",
abstract = "Using modern analytical techniques, a comprehensive study of the chemical composition of fruits from apple cultivars grown in Western Norway during 2019 and 2020 was done. Metals, sugars, organic acids, antioxidant tests, and polyphenol content have been observed. In all investigated samples, the most dominant sugars were glucose, fructose, and sucrose. Among 11 tested organic acids, the dominant was malic acid, followed by citric and maleic acid. The most common metal was potassium, followed by magnesium and zinc. The quantification of polyphenols showed that among the 11 quantified polyphenols, chlorogenic acid, quercetin 3- O- rhamnoside, quercetin 3 -O -glucoside, quercetin, and phlorizin were the most abundant. A detailed study of the polyphenolic profile of nine investigated apple samples provided 30 identified polyphenolic compounds from the class of hydroxybenzoic and hydroxycinnamic acids, flavonoids, and dihydrochalcones. In addition to the identified 3- O -caffeoylquinic acid, its two isomers of 5- O -caffeoylquinic acid and three esters were also found. Present polyphenols of the tested apples provided significant data on the quality of Norwegian apples, and they contribute to the distinguishing of these apple samples.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars",
volume = "9",
doi = "10.3389/fnut.2022.941487",
pages = "941487"
}

Fotirić Akšić, M., Nešović, M., Ćirić, I., Tešić, Ž., Pezo, L., Tosti, T., Gašić, U., Dojčinović, B., Lončar, B.,& Meland, M.. (2022). Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 941487.
https://doi.org/10.3389/fnut.2022.941487

Fotirić Akšić M, Nešović M, Ćirić I, Tešić Ž, Pezo L, Tosti T, Gašić U, Dojčinović B, Lončar B, Meland M. Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars. in Frontiers in Nutrition. 2022;9:941487.
doi:10.3389/fnut.2022.941487 .

Fotirić Akšić, Milica, Nešović, Milica, Ćirić, Ivanka, Tešić, Živoslav, Pezo, Lato, Tosti, Tomislav, Gašić, Uroš, Dojčinović, Biljana, Lončar, Biljana, Meland, Mekjell, "Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars" in Frontiers in Nutrition, 9 (2022):941487,
https://doi.org/10.3389/fnut.2022.941487 . .

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav
AU  - Vovk, Irena
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.941463/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9263698
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5080
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.
VL  - 9
DO  - 10.3389/fnut.2022.941463
SP  - 941463
ER  - 

@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.",
volume = "9",
doi = "10.3389/fnut.2022.941463",
pages = "941463"
}

Nedić, N., Nešović, M., Radišić, P., Gašić, U., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463

Nedić N, Nešović M, Radišić P, Gašić U, Baošić R, Joksimović K, Pezo L, Tešić Ž, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .

Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš, Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav, Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia." in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2210803322000495
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5012
AB  - Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
SP  - 100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
doi = "10.1016/j.hermed.2022.100580",
pages = "100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

TY  - JOUR
AU  - Ćirković, Dušica
AU  - Matijašević, Saša
AU  - Ćirković, Bratislav
AU  - Laketić, Darko
AU  - Jovanović, Zoran
AU  - Kostić, Boban
AU  - Bešlić, Zoran
AU  - Sredojević, Milica
AU  - Tešić, Živoslav
AU  - Banjanac, Tijana
AU  - Gašić, Uroš
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/4/296
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4959
AB  - The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which produces quality wines of characteristic and unique tastes. In the agroecological conditions of the Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine produced from the variety Prokupac was examined. Four experimental treatments with different timings of manual defoliation were applied: early defoliation—treatment I, early defoliation—treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine was determined for the three treatments of defoliation and the control treatment. Additionally, a multivariate analysis was applied on the obtained results, together with already published data (grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained, while the treatments with early defoliation did not differ in relation to the control sample. Early defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins (TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)
IS  - 4
VL  - 8
DO  - 10.3390/horticulturae8040296
SP  - 296
ER  - 

@article{
author = "Ćirković, Dušica and Matijašević, Saša and Ćirković, Bratislav and Laketić, Darko and Jovanović, Zoran and Kostić, Boban and Bešlić, Zoran and Sredojević, Milica and Tešić, Živoslav and Banjanac, Tijana and Gašić, Uroš",
year = "2022",
abstract = "The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which produces quality wines of characteristic and unique tastes. In the agroecological conditions of the Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine produced from the variety Prokupac was examined. Four experimental treatments with different timings of manual defoliation were applied: early defoliation—treatment I, early defoliation—treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine was determined for the three treatments of defoliation and the control treatment. Additionally, a multivariate analysis was applied on the obtained results, together with already published data (grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained, while the treatments with early defoliation did not differ in relation to the control sample. Early defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins (TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)",
number = "4",
volume = "8",
doi = "10.3390/horticulturae8040296",
pages = "296"
}

Ćirković, D., Matijašević, S., Ćirković, B., Laketić, D., Jovanović, Z., Kostić, B., Bešlić, Z., Sredojević, M., Tešić, Ž., Banjanac, T.,& Gašić, U.. (2022). Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae
Basel: MDPI., 8(4), 296.
https://doi.org/10.3390/horticulturae8040296

Ćirković D, Matijašević S, Ćirković B, Laketić D, Jovanović Z, Kostić B, Bešlić Z, Sredojević M, Tešić Ž, Banjanac T, Gašić U. Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae. 2022;8(4):296.
doi:10.3390/horticulturae8040296 .

Ćirković, Dušica, Matijašević, Saša, Ćirković, Bratislav, Laketić, Darko, Jovanović, Zoran, Kostić, Boban, Bešlić, Zoran, Sredojević, Milica, Tešić, Živoslav, Banjanac, Tijana, Gašić, Uroš, "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)" in Horticulturae, 8, no. 4 (2022):296,
https://doi.org/10.3390/horticulturae8040296 . .

TY  - JOUR
AU  - Brčić Karačonji, Irena
AU  - Jurica, Karlo
AU  - Gašić, Uroš
AU  - Dramićanin, Aleksandra
AU  - Tešić, Živoslav
AU  - Milojković Opsenica, Dušanka
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/1/25
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8747429
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4758
AB  - The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial, antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was determined by Folin-Ciocalteu's assay, while RSA was investigated using DPPH reagent. A total of 64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were the major compounds found in fruits. To the authors' knowledge, 16 and 5 phenolics in leaves and fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of 67.07-104.74 and 16.78-25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA for leaves and fruits were in the ranges of 408.92-430.98 and 74.30-104.04 μmol Trolox equivalents (TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves. Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to A. unedo as a promising health-promoting plant and natural food preservative.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.
IS  - 1
VL  - 11
DO  - 10.3390/plants11010025
SP  - 25
ER  - 

@article{
author = "Brčić Karačonji, Irena and Jurica, Karlo and Gašić, Uroš and Dramićanin, Aleksandra and Tešić, Živoslav and Milojković Opsenica, Dušanka",
year = "2022",
abstract = "The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial, antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was determined by Folin-Ciocalteu's assay, while RSA was investigated using DPPH reagent. A total of 64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were the major compounds found in fruits. To the authors' knowledge, 16 and 5 phenolics in leaves and fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of 67.07-104.74 and 16.78-25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA for leaves and fruits were in the ranges of 408.92-430.98 and 74.30-104.04 μmol Trolox equivalents (TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves. Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to A. unedo as a promising health-promoting plant and natural food preservative.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.",
number = "1",
volume = "11",
doi = "10.3390/plants11010025",
pages = "25"
}

Brčić Karačonji, I., Jurica, K., Gašić, U., Dramićanin, A., Tešić, Ž.,& Milojković Opsenica, D.. (2022). Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(1), 25.
https://doi.org/10.3390/plants11010025

Brčić Karačonji I, Jurica K, Gašić U, Dramićanin A, Tešić Ž, Milojković Opsenica D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.. in Plants (Basel, Switzerland). 2022;11(1):25.
doi:10.3390/plants11010025 .

Brčić Karačonji, Irena, Jurica, Karlo, Gašić, Uroš, Dramićanin, Aleksandra, Tešić, Živoslav, Milojković Opsenica, Dušanka, "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits." in Plants (Basel, Switzerland), 11, no. 1 (2022):25,
https://doi.org/10.3390/plants11010025 . .

TY  - JOUR
AU  - Bugeja Douglas, Adrian
AU  - Nešović, Milica
AU  - Šikoparija, Branko
AU  - Radišić, Predrag
AU  - Tosti, Tomislav
AU  - Trifković, Jelena
AU  - Russi, Luigi
AU  - Attard, Everaldo
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5665
AB  - Malta, a country renowned for its honey, has not been extensively mentioned in studies based on honey. In addition to many parameters, the collection period affects honey quality, precisely due to the different floral composition that exists during a certain season. Therefore, the significance of this study refers to the provision of data on honey from Malta collected during the autumn, spring, and summer seasons. Melissopalynological analysis, determination of physicochemical parameters, and the use of analytical chromatographic methods enabled detailed analysis of this honey. Principal component analysis (PCA) provided the differentiation of Maltese honey depending on the harvest season. Lotus pollen, followed by Eucalyptus, predominated in all honey samples. Characteristic compounds for summer honey were pinocembrin, galangin, kaempferol, chrysin, p-hydroxybenzoic acid, vanillic acid and maltotriose, while quercetin 3-O-galactoside, ferulic acid, ellagic acid, protocatechuic acid, luteolin 7-O-glucoside and melibiose were specific for autumn honey. A higher amount of p-coumaric acid, genistein, catechin, as well as the content of many sugars were found in spring samples. To the best of our knowledge, this is the first scientific work dealing with a detailed chemical analysis of Maltese honey.
PB  - Belgrade: Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons
IS  - 9
VL  - 87
DO  - 10.2298/JSC211214033B
SP  - 983
EP  - 995
ER  - 

@article{
author = "Bugeja Douglas, Adrian and Nešović, Milica and Šikoparija, Branko and Radišić, Predrag and Tosti, Tomislav and Trifković, Jelena and Russi, Luigi and Attard, Everaldo and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Malta, a country renowned for its honey, has not been extensively mentioned in studies based on honey. In addition to many parameters, the collection period affects honey quality, precisely due to the different floral composition that exists during a certain season. Therefore, the significance of this study refers to the provision of data on honey from Malta collected during the autumn, spring, and summer seasons. Melissopalynological analysis, determination of physicochemical parameters, and the use of analytical chromatographic methods enabled detailed analysis of this honey. Principal component analysis (PCA) provided the differentiation of Maltese honey depending on the harvest season. Lotus pollen, followed by Eucalyptus, predominated in all honey samples. Characteristic compounds for summer honey were pinocembrin, galangin, kaempferol, chrysin, p-hydroxybenzoic acid, vanillic acid and maltotriose, while quercetin 3-O-galactoside, ferulic acid, ellagic acid, protocatechuic acid, luteolin 7-O-glucoside and melibiose were specific for autumn honey. A higher amount of p-coumaric acid, genistein, catechin, as well as the content of many sugars were found in spring samples. To the best of our knowledge, this is the first scientific work dealing with a detailed chemical analysis of Maltese honey.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons",
number = "9",
volume = "87",
doi = "10.2298/JSC211214033B",
pages = "983-995"
}

Bugeja Douglas, A., Nešović, M., Šikoparija, B., Radišić, P., Tosti, T., Trifković, J., Russi, L., Attard, E., Tešić, Ž.,& Gašić, U.. (2022). Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Society., 87(9), 983-995.
https://doi.org/10.2298/JSC211214033B

Bugeja Douglas A, Nešović M, Šikoparija B, Radišić P, Tosti T, Trifković J, Russi L, Attard E, Tešić Ž, Gašić U. Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons. in Journal of the Serbian Chemical Society. 2022;87(9):983-995.
doi:10.2298/JSC211214033B .

Bugeja Douglas, Adrian, Nešović, Milica, Šikoparija, Branko, Radišić, Predrag, Tosti, Tomislav, Trifković, Jelena, Russi, Luigi, Attard, Everaldo, Tešić, Živoslav, Gašić, Uroš, "Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons" in Journal of the Serbian Chemical Society, 87, no. 9 (2022):983-995,
https://doi.org/10.2298/JSC211214033B . .

TY  - JOUR
AU  - Fermo, Paola
AU  - Comite, Valeria
AU  - Sredojević, Milica
AU  - Ćirić, Ivanka
AU  - Gašić, Uroš
AU  - Mutić, Jelena
AU  - Baošić, Rada
AU  - Tešić, Živoslav
PY  - 2021
UR  - internal-pdf://Fermo et al. - 2021 - Elemental Analysis and Phenolic Profiles of Selected Italian Wines.pdf
UR  - https://www.mdpi.com/2304-8158/10/1/158
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4160
AB  - The study of the chemical composition of wines is nowadays a topic of great interest because of the importance of this market, especially in Italy, and also considering the numerous cases of falsification of famous and very expensive wines. The present paper focused on the analysis of metals and polyphenols in Italian wines belonging to different provenance and types. At this purpose 20 elements were quantified by inductively coupled plasma optical emission spectrometry (ICP-OES) and ICP mass spectrometry (ICP-MS). Regarding polyphenols, a total of 32 were quantified, among 6 were anthocyanins. Furthermore, in 4 samples (1 rosè and 3 red wines) 42 anthocyanins and related compounds were identified by ultra-high performance liquid chromatography (UHPLC)-Orbitrap MS technique (among these, 6 were also quantified). Non-anthocyanins were determined using UHPLC coupled with a diode array detector and triple-quadrupole mass spectrometer (UHPLC–DAD-QqQ-MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were measured using spectrophotometric methods. The results obtained by elemental techniques were submitted to principal components analysis (PCA) allowing to get information on both geographical and botanical origin of the examined wine samples. Some polyphenols have been detected in higher concentrations only in a certain type of wine, as for example in the case of Grechetto wine. Most of the identified anthocyanin derivatives (pyranoanthocyanins) are formed during the aging of wine by reaction with the other wine components.
PB  - MDPI AG
T2  - Foods
T1  - Elemental Analysis and Phenolic Profiles of Selected Italian Wines
IS  - 1
VL  - 10
DO  - 10.3390/foods10010158
SP  - 158
ER  - 

@article{
author = "Fermo, Paola and Comite, Valeria and Sredojević, Milica and Ćirić, Ivanka and Gašić, Uroš and Mutić, Jelena and Baošić, Rada and Tešić, Živoslav",
year = "2021",
abstract = "The study of the chemical composition of wines is nowadays a topic of great interest because of the importance of this market, especially in Italy, and also considering the numerous cases of falsification of famous and very expensive wines. The present paper focused on the analysis of metals and polyphenols in Italian wines belonging to different provenance and types. At this purpose 20 elements were quantified by inductively coupled plasma optical emission spectrometry (ICP-OES) and ICP mass spectrometry (ICP-MS). Regarding polyphenols, a total of 32 were quantified, among 6 were anthocyanins. Furthermore, in 4 samples (1 rosè and 3 red wines) 42 anthocyanins and related compounds were identified by ultra-high performance liquid chromatography (UHPLC)-Orbitrap MS technique (among these, 6 were also quantified). Non-anthocyanins were determined using UHPLC coupled with a diode array detector and triple-quadrupole mass spectrometer (UHPLC–DAD-QqQ-MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were measured using spectrophotometric methods. The results obtained by elemental techniques were submitted to principal components analysis (PCA) allowing to get information on both geographical and botanical origin of the examined wine samples. Some polyphenols have been detected in higher concentrations only in a certain type of wine, as for example in the case of Grechetto wine. Most of the identified anthocyanin derivatives (pyranoanthocyanins) are formed during the aging of wine by reaction with the other wine components.",
publisher = "MDPI AG",
journal = "Foods",
title = "Elemental Analysis and Phenolic Profiles of Selected Italian Wines",
number = "1",
volume = "10",
doi = "10.3390/foods10010158",
pages = "158"
}

Fermo, P., Comite, V., Sredojević, M., Ćirić, I., Gašić, U., Mutić, J., Baošić, R.,& Tešić, Ž.. (2021). Elemental Analysis and Phenolic Profiles of Selected Italian Wines. in Foods
MDPI AG., 10(1), 158.
https://doi.org/10.3390/foods10010158

Fermo P, Comite V, Sredojević M, Ćirić I, Gašić U, Mutić J, Baošić R, Tešić Ž. Elemental Analysis and Phenolic Profiles of Selected Italian Wines. in Foods. 2021;10(1):158.
doi:10.3390/foods10010158 .

Fermo, Paola, Comite, Valeria, Sredojević, Milica, Ćirić, Ivanka, Gašić, Uroš, Mutić, Jelena, Baošić, Rada, Tešić, Živoslav, "Elemental Analysis and Phenolic Profiles of Selected Italian Wines" in Foods, 10, no. 1 (2021):158,
https://doi.org/10.3390/foods10010158 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4314
AB  - The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.
PB  - Cambridge: Royal Society of Chemistry
T2  - RSC Advances
T1  - Distribution of polyphenolic and sugar compounds in different buckwheat plant parts
IS  - 42
VL  - 11
DO  - 10.1039/D1RA04250E
SP  - 25816
EP  - 25829
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2021",
abstract = "The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "RSC Advances",
title = "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts",
number = "42",
volume = "11",
doi = "10.1039/D1RA04250E",
pages = "25816-25829"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2021). Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances
Cambridge: Royal Society of Chemistry., 11(42), 25816-25829.
https://doi.org/10.1039/D1RA04250E

Nešović M, Gašić U, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić Ž. Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances. 2021;11(42):25816-25829.
doi:10.1039/D1RA04250E .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts" in RSC Advances, 11, no. 42 (2021):25816-25829,
https://doi.org/10.1039/D1RA04250E . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4315
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen
IS  - 7
VL  - 10
DO  - 10.3390/antiox10071091
SP  - 1091
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Nedić, Nebojša and Gašić, Uroš and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav and Pešić, Mirjana",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen",
number = "7",
volume = "10",
doi = "10.3390/antiox10071091",
pages = "1091"
}

Kostić, A., Milinčić, D., Nedić, N., Gašić, U., Špirović Trifunović, B., Vojt, D., Tešić, Ž.,& Pešić, M.. (2021). Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants
Basel: MDPI., 10(7), 1091.
https://doi.org/10.3390/antiox10071091

Kostić A, Milinčić D, Nedić N, Gašić U, Špirović Trifunović B, Vojt D, Tešić Ž, Pešić M. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .

Kostić, Aleksandar, Milinčić, Danijel, Nedić, Nebojša, Gašić, Uroš, Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav, Pešić, Mirjana, "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .

TY  - JOUR
AU  - Milinčić, Danijel
AU  - Kostić, Aleksandar
AU  - Gašić, Uroš
AU  - Lević, Steva
AU  - Stanojević, Sladjana
AU  - Barać, Miroljub
AU  - Tešić, Živoslav
AU  - Nedović, Viktor
AU  - Pešić, Mirjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4316
AB  - The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties
IS  - 7
VL  - 11
DO  - 10.3390/biom11070965
SP  - 965
ER  - 

@article{
author = "Milinčić, Danijel and Kostić, Aleksandar and Gašić, Uroš and Lević, Steva and Stanojević, Sladjana and Barać, Miroljub and Tešić, Živoslav and Nedović, Viktor and Pešić, Mirjana",
year = "2021",
abstract = "The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties",
number = "7",
volume = "11",
doi = "10.3390/biom11070965",
pages = "965"
}

Milinčić, D., Kostić, A., Gašić, U., Lević, S., Stanojević, S., Barać, M., Tešić, Ž., Nedović, V.,& Pešić, M.. (2021). Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules
Basel: MDPI., 11(7), 965.
https://doi.org/10.3390/biom11070965

Milinčić D, Kostić A, Gašić U, Lević S, Stanojević S, Barać M, Tešić Ž, Nedović V, Pešić M. Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules. 2021;11(7):965.
doi:10.3390/biom11070965 .

Milinčić, Danijel, Kostić, Aleksandar, Gašić, Uroš, Lević, Steva, Stanojević, Sladjana, Barać, Miroljub, Tešić, Živoslav, Nedović, Viktor, Pešić, Mirjana, "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties" in Biomolecules, 11, no. 7 (2021):965,
https://doi.org/10.3390/biom11070965 . .

TY  - JOUR
AU  - Skočajić, Dragana
AU  - Gašić, Uroš
AU  - Dabić Zagorac, Dragana
AU  - Nešić, Marija
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
AU  - Fotirić Akšić, Milica
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/12/2822
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8706243
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4759
AB  - The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.
IS  - 12
VL  - 10
DO  - 10.3390/plants10122822
SP  - 2822
ER  - 

@article{
author = "Skočajić, Dragana and Gašić, Uroš and Dabić Zagorac, Dragana and Nešić, Marija and Tešić, Živoslav and Meland, Mekjell and Fotirić Akšić, Milica",
year = "2021",
abstract = "The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.",
number = "12",
volume = "10",
doi = "10.3390/plants10122822",
pages = "2822"
}

Skočajić, D., Gašić, U., Dabić Zagorac, D., Nešić, M., Tešić, Ž., Meland, M.,& Fotirić Akšić, M.. (2021). Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.. in Plants (Basel, Switzerland)
Basel: MDPI., 10(12), 2822.
https://doi.org/10.3390/plants10122822

Skočajić D, Gašić U, Dabić Zagorac D, Nešić M, Tešić Ž, Meland M, Fotirić Akšić M. Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.. in Plants (Basel, Switzerland). 2021;10(12):2822.
doi:10.3390/plants10122822 .

Skočajić, Dragana, Gašić, Uroš, Dabić Zagorac, Dragana, Nešić, Marija, Tešić, Živoslav, Meland, Mekjell, Fotirić Akšić, Milica, "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries." in Plants (Basel, Switzerland), 10, no. 12 (2021):2822,
https://doi.org/10.3390/plants10122822 . .

TY  - JOUR
AU  - Dabić Zagorac, Dragana
AU  - Fotirić Akšić, Milica
AU  - Galvnik, Vesna
AU  - Gašić, Uroš
AU  - Vovk, Irena
AU  - Tešić, Živoslav
AU  - Natić, Maja
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4066
AB  - The profile of flavan‐3‐ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan‐3‐ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra‐high‐performance liquid chromatography with linear trap quadrupole‐Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin‐layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
PB  - Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
IS  - 8
VL  - 43
DO  - 10.1002/jssc.201901271
SP  - 1431
EP  - 1439
ER  - 

@article{
author = "Dabić Zagorac, Dragana and Fotirić Akšić, Milica and Galvnik, Vesna and Gašić, Uroš and Vovk, Irena and Tešić, Živoslav and Natić, Maja",
year = "2020",
abstract = "The profile of flavan‐3‐ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan‐3‐ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra‐high‐performance liquid chromatography with linear trap quadrupole‐Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin‐layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
publisher = "Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species",
number = "8",
volume = "43",
doi = "10.1002/jssc.201901271",
pages = "1431-1439"
}

Dabić Zagorac, D., Fotirić Akšić, M., Galvnik, V., Gašić, U., Vovk, I., Tešić, Ž.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science
Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA., 43(8), 1431-1439.
https://doi.org/10.1002/jssc.201901271

Dabić Zagorac D, Fotirić Akšić M, Galvnik V, Gašić U, Vovk I, Tešić Ž, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439.
doi:10.1002/jssc.201901271 .

Dabić Zagorac, Dragana, Fotirić Akšić, Milica, Galvnik, Vesna, Gašić, Uroš, Vovk, Irena, Tešić, Živoslav, Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439,
https://doi.org/10.1002/jssc.201901271 . .

TY  - JOUR
AU  - Filip, Snežana
AU  - Đurović, Saša
AU  - Blagojević, Stevan
AU  - Tomić, Ana
AU  - Ranitović, Aleksandra
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Zeković, Zoran
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4317
AB  - The main goal of this study was to establish the chemical profile of Osage orange (Maclura pomifera) leaf extracts, obtained by conventional maceration technique, and to examine its antimicrobial activity. The identification and quantification of the extract compounds were done using ultra-high-performance liquid chromatography, with a diode array detector coupled with triple-quadrupole mass spectrometer and gas chromatography–mass spectrometry techniques. Thirty-one polyphenolic compounds were detected and identified in the ethanolic extracts, whereby 5-O-caffeoylquinic acid was found to be the dominant compound. Among other compounds, pentacosane and palmitic acid were the most abundant compounds in the dichloromethane extract. The preliminary antimicrobial activity screening shows that Gram-positive bacteria tend to be more sensitive to the investigated extracts. The highest antimicrobial activity was determined against Enterococcus faecalis ATCC 19433 and Listeria monocytogenes ATCC 35152. From these results, Osage orange leaves can be considered as plant material with significant antimicrobial properties.
PB  - Weinheim: Wiley-VCH Verlag Gmbh
T2  - Archiv der Pharmazie
T1  - Chemical composition and antimicrobial activity of Osage orange (Maclura pomifera) leaf extracts
IS  - 2
VL  - 354
DO  - 10.1002/ardp.202000195
SP  - 2000195
ER  - 

@article{
author = "Filip, Snežana and Đurović, Saša and Blagojević, Stevan and Tomić, Ana and Ranitović, Aleksandra and Gašić, Uroš and Tešić, Živoslav and Zeković, Zoran",
year = "2020",
abstract = "The main goal of this study was to establish the chemical profile of Osage orange (Maclura pomifera) leaf extracts, obtained by conventional maceration technique, and to examine its antimicrobial activity. The identification and quantification of the extract compounds were done using ultra-high-performance liquid chromatography, with a diode array detector coupled with triple-quadrupole mass spectrometer and gas chromatography–mass spectrometry techniques. Thirty-one polyphenolic compounds were detected and identified in the ethanolic extracts, whereby 5-O-caffeoylquinic acid was found to be the dominant compound. Among other compounds, pentacosane and palmitic acid were the most abundant compounds in the dichloromethane extract. The preliminary antimicrobial activity screening shows that Gram-positive bacteria tend to be more sensitive to the investigated extracts. The highest antimicrobial activity was determined against Enterococcus faecalis ATCC 19433 and Listeria monocytogenes ATCC 35152. From these results, Osage orange leaves can be considered as plant material with significant antimicrobial properties.",
publisher = "Weinheim: Wiley-VCH Verlag Gmbh",
journal = "Archiv der Pharmazie",
title = "Chemical composition and antimicrobial activity of Osage orange (Maclura pomifera) leaf extracts",
number = "2",
volume = "354",
doi = "10.1002/ardp.202000195",
pages = "2000195"
}

Filip, S., Đurović, S., Blagojević, S., Tomić, A., Ranitović, A., Gašić, U., Tešić, Ž.,& Zeković, Z.. (2020). Chemical composition and antimicrobial activity of Osage orange (Maclura pomifera) leaf extracts. in Archiv der Pharmazie
Weinheim: Wiley-VCH Verlag Gmbh., 354(2), 2000195.
https://doi.org/10.1002/ardp.202000195

Filip S, Đurović S, Blagojević S, Tomić A, Ranitović A, Gašić U, Tešić Ž, Zeković Z. Chemical composition and antimicrobial activity of Osage orange (Maclura pomifera) leaf extracts. in Archiv der Pharmazie. 2020;354(2):2000195.
doi:10.1002/ardp.202000195 .

Filip, Snežana, Đurović, Saša, Blagojević, Stevan, Tomić, Ana, Ranitović, Aleksandra, Gašić, Uroš, Tešić, Živoslav, Zeković, Zoran, "Chemical composition and antimicrobial activity of Osage orange (Maclura pomifera) leaf extracts" in Archiv der Pharmazie, 354, no. 2 (2020):2000195,
https://doi.org/10.1002/ardp.202000195 . .

TY  - JOUR
AU  - Gašić, Uroš
AU  - Ćirić, Ivanka
AU  - Pejčić, Tomislav
AU  - Radenković, Dejan
AU  - Đorđević, Vladimir
AU  - Radulović, Siniša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/6/547
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32585831
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7346180
UR  - https://radar.ibiss.bg.ac.rs/123456789/3891
AB  - Pancreatic cancer (PC) is very aggressive and it is estimated that it kills nearly 50% of patients within the first six months. The lack of symptoms specific to this disease prevents early diagnosis and treatment. Today, gemcitabine alone or in combination with other cytostatic agents such as cisplatin (Cis), 5-fluorouracil (5-FU), irinotecan, capecitabine, or oxaliplatin (Oxa) is used in conventional therapy. Outgoing literature provides data on the use of polyphenols, biologically active compounds, in the treatment of pancreatic cancer and the prevention of acute pancreatitis. Therefore, the first part of this review gives a brief overview of the state of pancreatic disease as well as the procedures for its treatment. The second part provides a detailed overview of the research regarding the anticancer effects of both pure polyphenols and their plant extracts. The results regarding the antiproliferative, antimetastatic, as well as inhibitory effects of polyphenols against PC cell lines as well as the prevention of acute pancreatitis are presented in detail. Finally, particular emphasis is given to the polyphenolic profiles of apples, berries, cherries, sour cherries, and grapes, given the fact that these fruits are rich in polyphenols and anthocyanins. Polyphenolic profiles, the content of individual polyphenols, and their relationships are discussed. Based on this, significant data can be obtained regarding the amount of fruit that should be consumed daily to achieve a therapeutic effect.
PB  - MDPI AG
T2  - Antioxidants (Basel, Switzerland)
T1  - Polyphenols as Possible Agents for Pancreatic Diseases.
IS  - 6
VL  - 9
DO  - 10.3390/antiox9060547
SP  - 547
ER  - 

@article{
author = "Gašić, Uroš and Ćirić, Ivanka and Pejčić, Tomislav and Radenković, Dejan and Đorđević, Vladimir and Radulović, Siniša and Tešić, Živoslav",
year = "2020",
abstract = "Pancreatic cancer (PC) is very aggressive and it is estimated that it kills nearly 50% of patients within the first six months. The lack of symptoms specific to this disease prevents early diagnosis and treatment. Today, gemcitabine alone or in combination with other cytostatic agents such as cisplatin (Cis), 5-fluorouracil (5-FU), irinotecan, capecitabine, or oxaliplatin (Oxa) is used in conventional therapy. Outgoing literature provides data on the use of polyphenols, biologically active compounds, in the treatment of pancreatic cancer and the prevention of acute pancreatitis. Therefore, the first part of this review gives a brief overview of the state of pancreatic disease as well as the procedures for its treatment. The second part provides a detailed overview of the research regarding the anticancer effects of both pure polyphenols and their plant extracts. The results regarding the antiproliferative, antimetastatic, as well as inhibitory effects of polyphenols against PC cell lines as well as the prevention of acute pancreatitis are presented in detail. Finally, particular emphasis is given to the polyphenolic profiles of apples, berries, cherries, sour cherries, and grapes, given the fact that these fruits are rich in polyphenols and anthocyanins. Polyphenolic profiles, the content of individual polyphenols, and their relationships are discussed. Based on this, significant data can be obtained regarding the amount of fruit that should be consumed daily to achieve a therapeutic effect.",
publisher = "MDPI AG",
journal = "Antioxidants (Basel, Switzerland)",
title = "Polyphenols as Possible Agents for Pancreatic Diseases.",
number = "6",
volume = "9",
doi = "10.3390/antiox9060547",
pages = "547"
}

Gašić, U., Ćirić, I., Pejčić, T., Radenković, D., Đorđević, V., Radulović, S.,& Tešić, Ž.. (2020). Polyphenols as Possible Agents for Pancreatic Diseases.. in Antioxidants (Basel, Switzerland)
MDPI AG., 9(6), 547.
https://doi.org/10.3390/antiox9060547

Gašić U, Ćirić I, Pejčić T, Radenković D, Đorđević V, Radulović S, Tešić Ž. Polyphenols as Possible Agents for Pancreatic Diseases.. in Antioxidants (Basel, Switzerland). 2020;9(6):547.
doi:10.3390/antiox9060547 .

Gašić, Uroš, Ćirić, Ivanka, Pejčić, Tomislav, Radenković, Dejan, Đorđević, Vladimir, Radulović, Siniša, Tešić, Živoslav, "Polyphenols as Possible Agents for Pancreatic Diseases." in Antioxidants (Basel, Switzerland), 9, no. 6 (2020):547,
https://doi.org/10.3390/antiox9060547 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Šikoparija, Branko
AU  - Nedić, Nebojša
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4058
AB  - A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.
PB  - Royal Society of Chemistry
T2  - Royal Society Open Science
T1  - Polyphenolic profile of buckwheat honey, nectar and pollen
IS  - 12
VL  - 7
DO  - 10.1098/rsos.201576
SP  - 201576
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Šikoparija, Branko and Nedić, Nebojša and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2020",
abstract = "A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.",
publisher = "Royal Society of Chemistry",
journal = "Royal Society Open Science",
title = "Polyphenolic profile of buckwheat honey, nectar and pollen",
number = "12",
volume = "7",
doi = "10.1098/rsos.201576",
pages = "201576"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Šikoparija, B., Nedić, N., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2020). Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science
Royal Society of Chemistry., 7(12), 201576.
https://doi.org/10.1098/rsos.201576

Nešović M, Gašić U, Tosti T, Horvacki N, Šikoparija B, Nedić N, Blagojević S, Ignjatović L, Tešić Ž. Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science. 2020;7(12):201576.
doi:10.1098/rsos.201576 .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Šikoparija, Branko, Nedić, Nebojša, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Polyphenolic profile of buckwheat honey, nectar and pollen" in Royal Society Open Science, 7, no. 12 (2020):201576,
https://doi.org/10.1098/rsos.201576 . .

TY  - JOUR
AU  - Đurović, Saša
AU  - Vujanović, Milena
AU  - Radojković, Marija
AU  - Filipović, Jelena
AU  - Filipović, Vladimir
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Mašković, Pavle
AU  - Zeković, Zoran
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4067
AB  - Stinging nettle is an annual plant. This plant is known for applications in folk medicine, and as the human diet. The stinging nettle leaves has been used to obtain extracts, which are expected to be with a high content of biologically active compounds. Prepared microwave extracts were applied in the formulation of the functional products. The article completes preparation of the bread with addition of the stinging nettle leaves and its extract, and bread’s composition in phenolic acids, flavonoids, micro elements, and macro elements. The biological activity of the prepared bread samples showed significant antioxidant activity. This was especially true against DPPH radicals. It has been shown that it holds a high cytotoxic activity. Leaves themselves decreased the quality of the bread, while the extract improved the quality. It was sensorially confirmed. The article concluded that the extract substitutes leaves in bread as a product of a high benefit.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread
VL  - 312
DO  - 10.1016/j.foodchem.2019.126091
SP  - 126091
ER  - 

@article{
author = "Đurović, Saša and Vujanović, Milena and Radojković, Marija and Filipović, Jelena and Filipović, Vladimir and Gašić, Uroš and Tešić, Živoslav and Mašković, Pavle and Zeković, Zoran",
year = "2020",
abstract = "Stinging nettle is an annual plant. This plant is known for applications in folk medicine, and as the human diet. The stinging nettle leaves has been used to obtain extracts, which are expected to be with a high content of biologically active compounds. Prepared microwave extracts were applied in the formulation of the functional products. The article completes preparation of the bread with addition of the stinging nettle leaves and its extract, and bread’s composition in phenolic acids, flavonoids, micro elements, and macro elements. The biological activity of the prepared bread samples showed significant antioxidant activity. This was especially true against DPPH radicals. It has been shown that it holds a high cytotoxic activity. Leaves themselves decreased the quality of the bread, while the extract improved the quality. It was sensorially confirmed. The article concluded that the extract substitutes leaves in bread as a product of a high benefit.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread",
volume = "312",
doi = "10.1016/j.foodchem.2019.126091",
pages = "126091"
}

Đurović, S., Vujanović, M., Radojković, M., Filipović, J., Filipović, V., Gašić, U., Tešić, Ž., Mašković, P.,& Zeković, Z.. (2020). The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread. in Food Chemistry
Elsevier Ltd., 312, 126091.
https://doi.org/10.1016/j.foodchem.2019.126091

Đurović S, Vujanović M, Radojković M, Filipović J, Filipović V, Gašić U, Tešić Ž, Mašković P, Zeković Z. The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread. in Food Chemistry. 2020;312:126091.
doi:10.1016/j.foodchem.2019.126091 .

Đurović, Saša, Vujanović, Milena, Radojković, Marija, Filipović, Jelena, Filipović, Vladimir, Gašić, Uroš, Tešić, Živoslav, Mašković, Pavle, Zeković, Zoran, "The functional food production: Application of stinging nettle leaves and its extracts in the baking of a bread" in Food Chemistry, 312 (2020):126091,
https://doi.org/10.1016/j.foodchem.2019.126091 . .

TY  - JOUR
AU  - Radović, Mirjana
AU  - Milatović, Dragan
AU  - Tešić, Živoslav
AU  - Tosti, Tomislav
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Dabić Zagorac, Dragana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3705
AB  - This paper presents the chemical composition of the fruits of three European plum cultivars (‘Cacanska Rana’, ‘Cacanska Lepotica’, and ‘Cacanska Najbolja’) grafted on four rootstocks (Myrobalan, ‘Pixy’, ‘Fereley’, and ‘St. Julien A’). The rootstock/cultivar combinations significantly influenced the content of most of the phenolic compounds in the plum skin and flesh. The total anthocyanin content in the skin ranged from 1.87 to 7.33 mg cyanidin-3-glucoside g−1 fresh weight. The major phenolic compound in the skin was rutin, which ranged from 95 to 238 mg kg−1, while in the flesh, cinnamic acid and catechin were dominant. The total sugar content in the fruit ranged from 66.2–147 mg g−1. The highest glucose and fructose contents were in ‘Cacanska Lepotica’, then in ‘Cacanska Najbolja’, and the lowest were in ‘Cacanska Rana’ cultivar. The highest contents of glucose and sucrose were found in fruits from trees grafted on Myrobalan rootstock, and the lowest in fruits from trees grafted on ‘St. Julien A’ rootstock. The rootstocks significantly influenced the potassium, calcium, and copper contents in the plum fruit. The results of this study demonstrate the significant effect of rootstocks on the phenolic and sugar profiles, and on the mineral content of the plum fruits.
PB  - Academic Press Inc.
T2  - Journal of Food Composition and Analysis
T1  - Influence of rootstocks on the chemical composition of the fruits of plum cultivars
VL  - 92
DO  - 10.1016/j.jfca.2020.103480
SP  - 103480
ER  - 

@article{
author = "Radović, Mirjana and Milatović, Dragan and Tešić, Živoslav and Tosti, Tomislav and Gašić, Uroš and Dojčinović, Biljana and Dabić Zagorac, Dragana",
year = "2020",
abstract = "This paper presents the chemical composition of the fruits of three European plum cultivars (‘Cacanska Rana’, ‘Cacanska Lepotica’, and ‘Cacanska Najbolja’) grafted on four rootstocks (Myrobalan, ‘Pixy’, ‘Fereley’, and ‘St. Julien A’). The rootstock/cultivar combinations significantly influenced the content of most of the phenolic compounds in the plum skin and flesh. The total anthocyanin content in the skin ranged from 1.87 to 7.33 mg cyanidin-3-glucoside g−1 fresh weight. The major phenolic compound in the skin was rutin, which ranged from 95 to 238 mg kg−1, while in the flesh, cinnamic acid and catechin were dominant. The total sugar content in the fruit ranged from 66.2–147 mg g−1. The highest glucose and fructose contents were in ‘Cacanska Lepotica’, then in ‘Cacanska Najbolja’, and the lowest were in ‘Cacanska Rana’ cultivar. The highest contents of glucose and sucrose were found in fruits from trees grafted on Myrobalan rootstock, and the lowest in fruits from trees grafted on ‘St. Julien A’ rootstock. The rootstocks significantly influenced the potassium, calcium, and copper contents in the plum fruit. The results of this study demonstrate the significant effect of rootstocks on the phenolic and sugar profiles, and on the mineral content of the plum fruits.",
publisher = "Academic Press Inc.",
journal = "Journal of Food Composition and Analysis",
title = "Influence of rootstocks on the chemical composition of the fruits of plum cultivars",
volume = "92",
doi = "10.1016/j.jfca.2020.103480",
pages = "103480"
}

Radović, M., Milatović, D., Tešić, Ž., Tosti, T., Gašić, U., Dojčinović, B.,& Dabić Zagorac, D.. (2020). Influence of rootstocks on the chemical composition of the fruits of plum cultivars. in Journal of Food Composition and Analysis
Academic Press Inc.., 92, 103480.
https://doi.org/10.1016/j.jfca.2020.103480

Radović M, Milatović D, Tešić Ž, Tosti T, Gašić U, Dojčinović B, Dabić Zagorac D. Influence of rootstocks on the chemical composition of the fruits of plum cultivars. in Journal of Food Composition and Analysis. 2020;92:103480.
doi:10.1016/j.jfca.2020.103480 .

Radović, Mirjana, Milatović, Dragan, Tešić, Živoslav, Tosti, Tomislav, Gašić, Uroš, Dojčinović, Biljana, Dabić Zagorac, Dragana, "Influence of rootstocks on the chemical composition of the fruits of plum cultivars" in Journal of Food Composition and Analysis, 92 (2020):103480,
https://doi.org/10.1016/j.jfca.2020.103480 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Trifković, Jelena
AU  - Baošić, Rada
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3600
AB  - The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.
T2  - RSC Advances
T1  - Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro
IS  - 5
VL  - 10
DO  - 10.1039/c9ra08783d
SP  - 2462
EP  - 2471
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Trifković, Jelena and Baošić, Rada and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2020",
abstract = "The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.",
journal = "RSC Advances",
title = "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro",
number = "5",
volume = "10",
doi = "10.1039/c9ra08783d",
pages = "2462-2471"
}

Nešović, M., Gašić, U., Tosti, T., Trifković, J., Baošić, R., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2020). Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances, 10(5), 2462-2471.
https://doi.org/10.1039/c9ra08783d

Nešović M, Gašić U, Tosti T, Trifković J, Baošić R, Blagojević S, Ignjatović L, Tešić Ž. Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances. 2020;10(5):2462-2471.
doi:10.1039/c9ra08783d .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Trifković, Jelena, Baošić, Rada, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro" in RSC Advances, 10, no. 5 (2020):2462-2471,
https://doi.org/10.1039/c9ra08783d . .

TY  - JOUR
AU  - Šuković, Danijela
AU  - Knežević, Bojana
AU  - Gašić, Uroš
AU  - Sredojević, Milica
AU  - Ćirić, Ivanka
AU  - Todić, Slavica
AU  - Mutić, Jelena
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://www.mdpi.com/2304-8158/9/2/138
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3607
AB  - Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.
T2  - Foods (Basel, Switzerland)
T1  - Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.
IS  - 2
VL  - 9
DO  - 10.3390/foods9020138
SP  - 138
ER  - 

@article{
author = "Šuković, Danijela and Knežević, Bojana and Gašić, Uroš and Sredojević, Milica and Ćirić, Ivanka and Todić, Slavica and Mutić, Jelena and Tešić, Živoslav",
year = "2020",
abstract = "Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.",
journal = "Foods (Basel, Switzerland)",
title = "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.",
number = "2",
volume = "9",
doi = "10.3390/foods9020138",
pages = "138"
}

Šuković, D., Knežević, B., Gašić, U., Sredojević, M., Ćirić, I., Todić, S., Mutić, J.,& Tešić, Ž.. (2020). Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.. in Foods (Basel, Switzerland), 9(2), 138.
https://doi.org/10.3390/foods9020138

Šuković D, Knežević B, Gašić U, Sredojević M, Ćirić I, Todić S, Mutić J, Tešić Ž. Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro.. in Foods (Basel, Switzerland). 2020;9(2):138.
doi:10.3390/foods9020138 .

Šuković, Danijela, Knežević, Bojana, Gašić, Uroš, Sredojević, Milica, Ćirić, Ivanka, Todić, Slavica, Mutić, Jelena, Tešić, Živoslav, "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitisvinifera L.) from Montenegro." in Foods (Basel, Switzerland), 9, no. 2 (2020):138,
https://doi.org/10.3390/foods9020138 . .

TY  - CONF
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5907
AB  - Introduction: Honey is a high energetic sweet food composed, among other components, of
bioactive compounds that attribute therapeutic properties of this product. As antioxidants,
polyphenols are recognized as the major constituents in honey responsible for its health
promoting properties. Bees produce a floral honey from the collected nectar of flowers, and
honeydew honey from collected excrement of insects or plants. Considering that Montenegro
is a country with traditional beekeeping, this is the first time to publish the results of its
honeydew and polyfloral honey samples.
Experimental: Analyzed honey samples, honeydew and polyfloral, were collected from three
locations in norther part of Montenegro, Berane, Bijelo Polje and Pljevlja. Polyphenol profile
of six honey samples was determinated by identifying polyphenols on UHPLC-LTQ OrbiTrap
MS, and by their quantifications on UHPLC-DAD MS/MS.
Results: Of the 32 identified polyphenol compounds, 24 were present in all samples. Appearing
the other eight polyphenol compounds has no constant diversity between the samples. The
similarity between them was confirmed by their quantifications. The highest proportion in total
polyphenols has a quercetin with a mean value of 3.82 mg/kg, followed by p-hydrohybenzoic
acid and p-coumaric acid. Slightly higher content of chrysin, pinocembrin, p-protocatechuic
and vanillic acid can be noticed. The quantifications of the target compounds include 33
polyphenols, and only 16 of them were previously identified. This indicates the occurrence of
certain dissimilarities between two used techniques, which are the consequences of similar
spectra of certain polyphenols and their derivatives.
Conclusion: This is the detailed study of polyphenols in Montenegrin honeys. Based on the
obtained results, it can be seen that the investigated honey samples are very rich in polyphenols,
with no significant differences between honeydew and polyfloral samples. Considering small
areas that cover these three regions of norther part of Montenegro, it can be assumed that small
diversity in plant species causes overlapping in honeybee’s pastures and similarity of samples
PB  - Novi Sad: Faculty of Technology
C3  - Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia
T1  - Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5907
ER  - 

@conference{
author = "Nešović, Milica and Gašić, Uroš and Tešić, Živoslav",
year = "2019",
abstract = "Introduction: Honey is a high energetic sweet food composed, among other components, of
bioactive compounds that attribute therapeutic properties of this product. As antioxidants,
polyphenols are recognized as the major constituents in honey responsible for its health
promoting properties. Bees produce a floral honey from the collected nectar of flowers, and
honeydew honey from collected excrement of insects or plants. Considering that Montenegro
is a country with traditional beekeeping, this is the first time to publish the results of its
honeydew and polyfloral honey samples.
Experimental: Analyzed honey samples, honeydew and polyfloral, were collected from three
locations in norther part of Montenegro, Berane, Bijelo Polje and Pljevlja. Polyphenol profile
of six honey samples was determinated by identifying polyphenols on UHPLC-LTQ OrbiTrap
MS, and by their quantifications on UHPLC-DAD MS/MS.
Results: Of the 32 identified polyphenol compounds, 24 were present in all samples. Appearing
the other eight polyphenol compounds has no constant diversity between the samples. The
similarity between them was confirmed by their quantifications. The highest proportion in total
polyphenols has a quercetin with a mean value of 3.82 mg/kg, followed by p-hydrohybenzoic
acid and p-coumaric acid. Slightly higher content of chrysin, pinocembrin, p-protocatechuic
and vanillic acid can be noticed. The quantifications of the target compounds include 33
polyphenols, and only 16 of them were previously identified. This indicates the occurrence of
certain dissimilarities between two used techniques, which are the consequences of similar
spectra of certain polyphenols and their derivatives.
Conclusion: This is the detailed study of polyphenols in Montenegrin honeys. Based on the
obtained results, it can be seen that the investigated honey samples are very rich in polyphenols,
with no significant differences between honeydew and polyfloral samples. Considering small
areas that cover these three regions of norther part of Montenegro, it can be assumed that small
diversity in plant species causes overlapping in honeybee’s pastures and similarity of samples",
publisher = "Novi Sad: Faculty of Technology",
journal = "Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia",
title = "Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5907"
}

Nešović, M., Gašić, U.,& Tešić, Ž.. (2019). Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro. in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia
Novi Sad: Faculty of Technology., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_5907

Nešović M, Gašić U, Tešić Ž. Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro. in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia. 2019;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_5907 .

Nešović, Milica, Gašić, Uroš, Tešić, Živoslav, "Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro" in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia (2019):39,
https://hdl.handle.net/21.15107/rcub_ibiss_5907 .