TY  - JOUR
AU  - Kasalović, Marijana P.
AU  - Jelača, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Lađarević, Jelena
AU  - Radovanović, Lidija
AU  - Božić, Bojan
AU  - Mijatović, Sanja
AU  - Pantelić, Nebojša Đ.
AU  - Kaluđerović, Goran N.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6268
AB  - Three new diphenyltin(IV) complexes, bis(3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoato)diphenyltin(IV) (1), bis(2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (2), and bis(2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (3), were synthesized and characterized by elemental microanalysis, FT-IR spectroscopy, and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. Crystal structure of ligand precursor, 3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoic acid (HL1), has been determined by X-ray diffraction studies. Asymmetric bidentate coordination of the carboxylato ligands and skew trapezoidal structures are assumed for the synthesized complexes. In vitro anticancer activity of the synthesized diphenyltin(IV) complexes was evaluated against three human: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three mouse tumor cell lines: 4 T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the range from 0.1 to 3.7 μM. Flow cytometric analysis and fluorescent microscopy suggest that complex 1 induces caspase-dependent apoptosis followed with strong blockade of cell division in HCT116 cells. Since complex 1 showed ROS/RNS scavenging potential mentioned cytotoxicity was not connected with oxidative stress.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies
VL  - 250
DO  - 10.1016/j.jinorgbio.2023.112399
SP  - 112399
ER  - 

@article{
author = "Kasalović, Marijana P. and Jelača, Sanja and Maksimović-Ivanić, Danijela and Lađarević, Jelena and Radovanović, Lidija and Božić, Bojan and Mijatović, Sanja and Pantelić, Nebojša Đ. and Kaluđerović, Goran N.",
year = "2024",
abstract = "Three new diphenyltin(IV) complexes, bis(3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoato)diphenyltin(IV) (1), bis(2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (2), and bis(2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato)diphenyltin(IV) (3), were synthesized and characterized by elemental microanalysis, FT-IR spectroscopy, and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. Crystal structure of ligand precursor, 3-(4-methyl-2-oxoquinolinyl-1(2H)-yl)propanoic acid (HL1), has been determined by X-ray diffraction studies. Asymmetric bidentate coordination of the carboxylato ligands and skew trapezoidal structures are assumed for the synthesized complexes. In vitro anticancer activity of the synthesized diphenyltin(IV) complexes was evaluated against three human: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three mouse tumor cell lines: 4 T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the range from 0.1 to 3.7 μM. Flow cytometric analysis and fluorescent microscopy suggest that complex 1 induces caspase-dependent apoptosis followed with strong blockade of cell division in HCT116 cells. Since complex 1 showed ROS/RNS scavenging potential mentioned cytotoxicity was not connected with oxidative stress.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies",
volume = "250",
doi = "10.1016/j.jinorgbio.2023.112399",
pages = "112399"
}

Kasalović, M. P., Jelača, S., Maksimović-Ivanić, D., Lađarević, J., Radovanović, L., Božić, B., Mijatović, S., Pantelić, N. Đ.,& Kaluđerović, G. N.. (2024). Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies. in Journal of Inorganic Biochemistry
Elsevier., 250, 112399.
https://doi.org/10.1016/j.jinorgbio.2023.112399

Kasalović MP, Jelača S, Maksimović-Ivanić D, Lađarević J, Radovanović L, Božić B, Mijatović S, Pantelić NĐ, Kaluđerović GN. Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies. in Journal of Inorganic Biochemistry. 2024;250:112399.
doi:10.1016/j.jinorgbio.2023.112399 .

Kasalović, Marijana P., Jelača, Sanja, Maksimović-Ivanić, Danijela, Lađarević, Jelena, Radovanović, Lidija, Božić, Bojan, Mijatović, Sanja, Pantelić, Nebojša Đ., Kaluđerović, Goran N., "Novel diphenyltin(IV) complexes with carboxylato N-functionalized 2- quinolone ligands: Synthesis, characterization and in vitro anticancer studies" in Journal of Inorganic Biochemistry, 250 (2024):112399,
https://doi.org/10.1016/j.jinorgbio.2023.112399 . .

TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Lunić, Tanja
AU  - Graovac, Stefan
AU  - Mandić, Marija
AU  - Repac, Jelena
AU  - Gašić, Uroš
AU  - Božić Nedeljković, Biljana
AU  - Božić, Bojan
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022006835
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5013
AB  - Diabetes mellitus has been recognized as one of the most challenging public health problems in the 21st century. The present study aimed to investigate the antidiabetic potential of 18 selected Lamiaceae representatives on yeast α-glucosidase and further translate experimental data into predicted human α-glucosidase inhibition. Since conventional antidiabetic drugs exhibit numerous unavoidable side effects, plant extracts are being increasingly explored as safer alternatives. Plant extracts were prepared by maceration, characterized by liquid chromatography-mass spectrometry (LC-MS), and subjected to in vitro determination of yeast α-glucosidase inhibitory potential, after which the kinetic study of one of the most potent extracts (Mentha × piperita) was performed. Docking simulations were done on both yeast and human α-glucosidase to explore binding modes and energies of the secondary metabolites identified in the examined extracts. Finally, the dynamical model has been constructed (adjR2 = 0.9885) and applied to explore extracts’ inhibition potential onto the human α-glucosidase. The constructed dynamical model revealed, for the first time, that different extracts of Satureja montana represent highly promising candidates for the prevention/treatment of diabetes mellitus. Therefore, the dynamical model described in this study represents a useful tool and a significant advance in the discovery of novel therapeutics with antidiabetic potential in humans.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling
VL  - 186
DO  - 10.1016/j.indcrop.2022.115200
SP  - 115200
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Lunić, Tanja and Graovac, Stefan and Mandić, Marija and Repac, Jelena and Gašić, Uroš and Božić Nedeljković, Biljana and Božić, Bojan",
year = "2022",
abstract = "Diabetes mellitus has been recognized as one of the most challenging public health problems in the 21st century. The present study aimed to investigate the antidiabetic potential of 18 selected Lamiaceae representatives on yeast α-glucosidase and further translate experimental data into predicted human α-glucosidase inhibition. Since conventional antidiabetic drugs exhibit numerous unavoidable side effects, plant extracts are being increasingly explored as safer alternatives. Plant extracts were prepared by maceration, characterized by liquid chromatography-mass spectrometry (LC-MS), and subjected to in vitro determination of yeast α-glucosidase inhibitory potential, after which the kinetic study of one of the most potent extracts (Mentha × piperita) was performed. Docking simulations were done on both yeast and human α-glucosidase to explore binding modes and energies of the secondary metabolites identified in the examined extracts. Finally, the dynamical model has been constructed (adjR2 = 0.9885) and applied to explore extracts’ inhibition potential onto the human α-glucosidase. The constructed dynamical model revealed, for the first time, that different extracts of Satureja montana represent highly promising candidates for the prevention/treatment of diabetes mellitus. Therefore, the dynamical model described in this study represents a useful tool and a significant advance in the discovery of novel therapeutics with antidiabetic potential in humans.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling",
volume = "186",
doi = "10.1016/j.indcrop.2022.115200",
pages = "115200"
}

Oalđe Pavlović, M., Lunić, T., Graovac, S., Mandić, M., Repac, J., Gašić, U., Božić Nedeljković, B.,& Božić, B.. (2022). Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling. in Industrial Crops and Products
Elsevier B.V.., 186, 115200.
https://doi.org/10.1016/j.indcrop.2022.115200

Oalđe Pavlović M, Lunić T, Graovac S, Mandić M, Repac J, Gašić U, Božić Nedeljković B, Božić B. Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling. in Industrial Crops and Products. 2022;186:115200.
doi:10.1016/j.indcrop.2022.115200 .

Oalđe Pavlović, Mariana, Lunić, Tanja, Graovac, Stefan, Mandić, Marija, Repac, Jelena, Gašić, Uroš, Božić Nedeljković, Biljana, Božić, Bojan, "Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling" in Industrial Crops and Products, 186 (2022):115200,
https://doi.org/10.1016/j.indcrop.2022.115200 . .

TY  - JOUR
AU  - Mandić, Marija
AU  - Mitić, Katarina
AU  - Nedeljković, Predrag
AU  - Perić, Mina
AU  - Božić, Bojan
AU  - Lunić, Tanja
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2022
UR  - https://www.mdpi.com/2072-6643/14/6/1273
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35334928
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8955508
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4940
AB  - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.
IS  - 6
VL  - 14
DO  - 10.3390/nu14061273
SP  - 1273
ER  - 

@article{
author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Božić, Bojan and Lunić, Tanja and Bačić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2022",
abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.",
number = "6",
volume = "14",
doi = "10.3390/nu14061273",
pages = "1273"
}

Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Božić, B., Lunić, T., Bačić, A., Rajilić-Stojanović, M., Peković, S.,& Božić Nedeljković, B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients
Basel: MDPI., 14(6), 1273.
https://doi.org/10.3390/nu14061273

Mandić M, Mitić K, Nedeljković P, Perić M, Božić B, Lunić T, Bačić A, Rajilić-Stojanović M, Peković S, Božić Nedeljković B. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients. 2022;14(6):1273.
doi:10.3390/nu14061273 .

Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Božić, Bojan, Lunić, Tanja, Bačić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis." in Nutrients, 14, no. 6 (2022):1273,
https://doi.org/10.3390/nu14061273 . .

TY  - CONF
AU  - Lunić, Tanja
AU  - Oalđe, Mariana
AU  - Mandić, Marija
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4106
AB  - Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
T1  - Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts
DO  - 10.3390/ECMC2020-07282
ER  - 

@conference{
author = "Lunić, Tanja and Oalđe, Mariana and Mandić, Marija and Sabovljević, Aneta and Sabovljević, Marko and Gašić, Uroš and Duletić-Laušević, Sonja and Božić, Bojan and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.",
title = "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts",
doi = "10.3390/ECMC2020-07282"
}

Lunić, T., Oalđe, M., Mandić, M., Sabovljević, A., Sabovljević, M., Gašić, U., Duletić-Laušević, S., Božić, B.,& Božić Nedeljković, B.. (2020). Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07282

Lunić T, Oalđe M, Mandić M, Sabovljević A, Sabovljević M, Gašić U, Duletić-Laušević S, Božić B, Božić Nedeljković B. Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.. 2020;.
doi:10.3390/ECMC2020-07282 .

Lunić, Tanja, Oalđe, Mariana, Mandić, Marija, Sabovljević, Aneta, Sabovljević, Marko, Gašić, Uroš, Duletić-Laušević, Sonja, Božić, Bojan, Božić Nedeljković, Biljana, "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts" in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online. (2020),
https://doi.org/10.3390/ECMC2020-07282 . .

TY  - CONF
AU  - Mandić, Marija
AU  - Oalđe, Mariana
AU  - Lunić, Tanja
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4105
AB  - Owing to structural diversity and potental pharmacological activity, bioactive compounds derived from natural sources are in the focus of intensive exploration. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. was examined, and further tested for antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative activity in vitro. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical analysis revealed the presence of phenolics, flavonoids, and triterpenoids as secondary metabolites of high biological activity. Antioxidant activity of extracts was assessed using three different methods, DPPH (2,2-diphenyl-1-picrylhydrazyl), total reducing power assay, and β-carotene/linoleic acid assay. To evaluate the enzyme-inhibitory activity, extracts were screened for α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase inhibitory effects. The biocompatibility of moss extracts was confirmed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts displayed significant antiproliferative activity (~ 50% inhibition) against the MDA-MB-231 (human breast adenocarcinoma) cells, which had not previously been reported elsewhere. The potential anti-neuroinflammatory activity was observed due to significant reduction of the NO production by LPS-stimulated BV2 (normal murine microglia cell line), being in line with previously recognized antineurodegenerative potential, as measured by the inhibition of acetylcholinesterase and tyrosinase activity. Furthermore, the H. ciliata extracts displayed significant antidiabetic effect via α-glucosidase inhibition. In general, the obtained results suggest the potential immunomodulatory activity of the moss H. ciliata extracts, remaining to be characterized in more detail in search for mechanisms underlying the immunomodulation processes.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online
T1  - Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential
DO  - 10.3390/ECMC2020-07281
ER  - 

@conference{
author = "Mandić, Marija and Oalđe, Mariana and Lunić, Tanja and Sabovljević, Aneta and Sabovljević, Marko and Gašić, Uroš and Duletić-Laušević, Sonja and Božić, Bojan and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Owing to structural diversity and potental pharmacological activity, bioactive compounds derived from natural sources are in the focus of intensive exploration. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. was examined, and further tested for antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative activity in vitro. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical analysis revealed the presence of phenolics, flavonoids, and triterpenoids as secondary metabolites of high biological activity. Antioxidant activity of extracts was assessed using three different methods, DPPH (2,2-diphenyl-1-picrylhydrazyl), total reducing power assay, and β-carotene/linoleic acid assay. To evaluate the enzyme-inhibitory activity, extracts were screened for α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase inhibitory effects. The biocompatibility of moss extracts was confirmed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts displayed significant antiproliferative activity (~ 50% inhibition) against the MDA-MB-231 (human breast adenocarcinoma) cells, which had not previously been reported elsewhere. The potential anti-neuroinflammatory activity was observed due to significant reduction of the NO production by LPS-stimulated BV2 (normal murine microglia cell line), being in line with previously recognized antineurodegenerative potential, as measured by the inhibition of acetylcholinesterase and tyrosinase activity. Furthermore, the H. ciliata extracts displayed significant antidiabetic effect via α-glucosidase inhibition. In general, the obtained results suggest the potential immunomodulatory activity of the moss H. ciliata extracts, remaining to be characterized in more detail in search for mechanisms underlying the immunomodulation processes.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online",
title = "Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential",
doi = "10.3390/ECMC2020-07281"
}

Mandić, M., Oalđe, M., Lunić, T., Sabovljević, A., Sabovljević, M., Gašić, U., Duletić-Laušević, S., Božić, B.,& Božić Nedeljković, B.. (2020). Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07281

Mandić M, Oalđe M, Lunić T, Sabovljević A, Sabovljević M, Gašić U, Duletić-Laušević S, Božić B, Božić Nedeljković B. Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online. 2020;.
doi:10.3390/ECMC2020-07281 .

Mandić, Marija, Oalđe, Mariana, Lunić, Tanja, Sabovljević, Aneta, Sabovljević, Marko, Gašić, Uroš, Duletić-Laušević, Sonja, Božić, Bojan, Božić Nedeljković, Biljana, "Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential" in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online (2020),
https://doi.org/10.3390/ECMC2020-07281 . .

TY  - JOUR
AU  - Petković Cvetković, Jelena
AU  - Božić, Bojan Đ.
AU  - Banjac, Nebojša R.
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Vitnik, Vesna D.
AU  - Vitnik, Željko J.
AU  - Ušćumlić, Gordana S.
AU  - Valentić, Nataša V.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0022286018315163?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3239
AB  - The macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine involved in inflammation, regulation of energy metabolism and glucocorticoid action. Chronic low-grade inflammation may be caused by fructose intake, contributing to visceral adipose tissue (VAT) dysfunction. Since MIF is a known antagonist of glucocorticoid signaling, and deregulated glucocorticoid signaling can contribute to lipid metabolism disturbances, we hypothesized that altered MIF signaling might underlie fructose-induced adiposity through glucocorticoid action. We analyzed physiological and biochemical parameters, adipose tissue histology, insulin sensitivity and lipid metabolism in wild type and MIF-/- C57Bl/6J mice consuming 20% fructose solution for 9 weeks. Glucocorticoid prereceptor metabolism and glucocorticoid receptor (GR) protein level were examined in VAT, together with the expression of glucocorticoid-target genes involved in lipid metabolism. The expression of adipogenic and lipogenic transcriptional regulators peroxisome proliferator activated receptor gamma (PPARG) and sterol regulatory element-binding protein 1c (SREBP1c) was also assessed. Results showed disturbed insulin sensitivity in all MIF-/- mice, regardless of the diet. Mice on fructose diet had increased energy intake, but increased visceral adiposity and enlarged adipocytes were observed only in fructose-fed MIF-/- mice. Increased VAT corticosterone level and 11 beta-hydroxysteroid dehydrogenase type 1, hexose-6-phosphate dehydrogenase and GR protein levels were observed in the same animals, together with induced expression of examined lipogenic genes and accumulation of PPARG and SREBP1c. In conclusion, the results showed that dietary fructose was associated with increased visceral adiposity through activation of GR-regulated lipogenic genes, but only in the absence of MIF, which set the state of hyperinsulinemia and insulin resistance.
T2  - Journal of Molecular Structure
T1  - Synthesis, antimicrobial activity and quantum chemical investigation of novel succinimide derivatives
VL  - 1181
DO  - 10.1016/j.molstruc.2018.12.083
SP  - 148
EP  - 156
ER  - 

@article{
author = "Petković Cvetković, Jelena and Božić, Bojan Đ. and Banjac, Nebojša R. and Petrović, Jovana and Soković, Marina and Vitnik, Vesna D. and Vitnik, Željko J. and Ušćumlić, Gordana S. and Valentić, Nataša V.",
year = "2019",
abstract = "The macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine involved in inflammation, regulation of energy metabolism and glucocorticoid action. Chronic low-grade inflammation may be caused by fructose intake, contributing to visceral adipose tissue (VAT) dysfunction. Since MIF is a known antagonist of glucocorticoid signaling, and deregulated glucocorticoid signaling can contribute to lipid metabolism disturbances, we hypothesized that altered MIF signaling might underlie fructose-induced adiposity through glucocorticoid action. We analyzed physiological and biochemical parameters, adipose tissue histology, insulin sensitivity and lipid metabolism in wild type and MIF-/- C57Bl/6J mice consuming 20% fructose solution for 9 weeks. Glucocorticoid prereceptor metabolism and glucocorticoid receptor (GR) protein level were examined in VAT, together with the expression of glucocorticoid-target genes involved in lipid metabolism. The expression of adipogenic and lipogenic transcriptional regulators peroxisome proliferator activated receptor gamma (PPARG) and sterol regulatory element-binding protein 1c (SREBP1c) was also assessed. Results showed disturbed insulin sensitivity in all MIF-/- mice, regardless of the diet. Mice on fructose diet had increased energy intake, but increased visceral adiposity and enlarged adipocytes were observed only in fructose-fed MIF-/- mice. Increased VAT corticosterone level and 11 beta-hydroxysteroid dehydrogenase type 1, hexose-6-phosphate dehydrogenase and GR protein levels were observed in the same animals, together with induced expression of examined lipogenic genes and accumulation of PPARG and SREBP1c. In conclusion, the results showed that dietary fructose was associated with increased visceral adiposity through activation of GR-regulated lipogenic genes, but only in the absence of MIF, which set the state of hyperinsulinemia and insulin resistance.",
journal = "Journal of Molecular Structure",
title = "Synthesis, antimicrobial activity and quantum chemical investigation of novel succinimide derivatives",
volume = "1181",
doi = "10.1016/j.molstruc.2018.12.083",
pages = "148-156"
}

Petković Cvetković, J., Božić, B. Đ., Banjac, N. R., Petrović, J., Soković, M., Vitnik, V. D., Vitnik, Ž. J., Ušćumlić, G. S.,& Valentić, N. V.. (2019). Synthesis, antimicrobial activity and quantum chemical investigation of novel succinimide derivatives. in Journal of Molecular Structure, 1181, 148-156.
https://doi.org/10.1016/j.molstruc.2018.12.083

Petković Cvetković J, Božić BĐ, Banjac NR, Petrović J, Soković M, Vitnik VD, Vitnik ŽJ, Ušćumlić GS, Valentić NV. Synthesis, antimicrobial activity and quantum chemical investigation of novel succinimide derivatives. in Journal of Molecular Structure. 2019;1181:148-156.
doi:10.1016/j.molstruc.2018.12.083 .

Petković Cvetković, Jelena, Božić, Bojan Đ., Banjac, Nebojša R., Petrović, Jovana, Soković, Marina, Vitnik, Vesna D., Vitnik, Željko J., Ušćumlić, Gordana S., Valentić, Nataša V., "Synthesis, antimicrobial activity and quantum chemical investigation of novel succinimide derivatives" in Journal of Molecular Structure, 1181 (2019):148-156,
https://doi.org/10.1016/j.molstruc.2018.12.083 . .