TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6693
AB  - Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.
PB  - Belgrade: Serbian Society for Microbiology
C3  - Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
T1  - Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6693
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2024",
abstract = "Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia",
title = "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6693"
}

Kolarević, S., Micsinai, A., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2024). Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
Belgrade: Serbian Society for Microbiology., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693

Kolarević S, Micsinai A, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia. 2024;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

Kolarević, Stoimir, Micsinai, Adrienn, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia" in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia (2024):65,
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

TY  - JOUR
AU  - Schachner-Gröhs, Iris
AU  - Koller, Michael
AU  - Leopold, Melanie
AU  - Kolm, Claudia
AU  - Linke, Rita
AU  - Jakwerth, Stefan
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kandler, Wolfgang
AU  - Sulyok, Michael
AU  - Vierheilig, Julia
AU  - Toumi, Marwene
AU  - Farkas, Rózsa
AU  - Toth, Erika
AU  - Kittinger, Clemens
AU  - Zarfel, Gernot
AU  - Farnleitner, Andreas
AU  - Kirschner, Alexander
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6581
AB  - The global spread of antimicrobial resistance (AMR) in the environment is a growing health threat. Large rivers are of particular concern as they are highly impacted by wastewater discharge while being vital lifelines serving various human needs. A comprehensive understanding of occurrence, spread and key drivers of AMR along whole river courses is largely lacking. We provide a holistic approach by studying spatiotemporal patterns and hotspots of antibiotic resistance genes (ARGs) along 2311 km of the navigable Danube River, combining a longitudinal and temporal monitoring campaign. The integration of advanced faecal pollution diagnostics and environmental and chemical key parameters allowed linking ARG concentrations to the major pollution sources and explaining the observed patterns. Nine AMR markers, including genes conferring resistance to five different antibiotic classes of clinical and environmental relevance, and one integrase gene were determined by probe-based qPCR. All AMR targets could be quantified in Danube River water, with intI1 and sul1 being ubiquitously abundant, qnrS, tetM, blaTEM with intermediate abundance and blaOXA-48like, blaCTX−M-1 group, blaCTX−M-9 group and blaKPC genes with rare occurrence. Human faecal pollution from municipal wastewater discharges was the dominant factor shaping ARG patterns along the Danube River. Other significant correlations of specific ARGs were observed with discharge, certain metals and pesticides. In contrast, intI1 was not associated with wastewater but was already established in the water microbiome. Animal contamination was detected only sporadically and was correlated with ARGs only in the temporal sampling set. During temporal monitoring, an extraordinary hotspot was identified emphasizing the variability within natural waters. This study provides the first comprehensive baseline concentrations of ARGs in the Danube River and lays the foundation for monitoring future trends and evaluating potential reduction measures. The applided holistic approach proved to be a valuable methodological contribution towards a better understanding of the environmental occurrence of AMR.
PB  - Elsevier
T2  - Water Research
T1  - Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River
VL  - 252
DO  - 10.1016/j.watres.2024.121244
SP  - 121244
ER  - 

@article{
author = "Schachner-Gröhs, Iris and Koller, Michael and Leopold, Melanie and Kolm, Claudia and Linke, Rita and Jakwerth, Stefan and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kandler, Wolfgang and Sulyok, Michael and Vierheilig, Julia and Toumi, Marwene and Farkas, Rózsa and Toth, Erika and Kittinger, Clemens and Zarfel, Gernot and Farnleitner, Andreas and Kirschner, Alexander",
year = "2024",
abstract = "The global spread of antimicrobial resistance (AMR) in the environment is a growing health threat. Large rivers are of particular concern as they are highly impacted by wastewater discharge while being vital lifelines serving various human needs. A comprehensive understanding of occurrence, spread and key drivers of AMR along whole river courses is largely lacking. We provide a holistic approach by studying spatiotemporal patterns and hotspots of antibiotic resistance genes (ARGs) along 2311 km of the navigable Danube River, combining a longitudinal and temporal monitoring campaign. The integration of advanced faecal pollution diagnostics and environmental and chemical key parameters allowed linking ARG concentrations to the major pollution sources and explaining the observed patterns. Nine AMR markers, including genes conferring resistance to five different antibiotic classes of clinical and environmental relevance, and one integrase gene were determined by probe-based qPCR. All AMR targets could be quantified in Danube River water, with intI1 and sul1 being ubiquitously abundant, qnrS, tetM, blaTEM with intermediate abundance and blaOXA-48like, blaCTX−M-1 group, blaCTX−M-9 group and blaKPC genes with rare occurrence. Human faecal pollution from municipal wastewater discharges was the dominant factor shaping ARG patterns along the Danube River. Other significant correlations of specific ARGs were observed with discharge, certain metals and pesticides. In contrast, intI1 was not associated with wastewater but was already established in the water microbiome. Animal contamination was detected only sporadically and was correlated with ARGs only in the temporal sampling set. During temporal monitoring, an extraordinary hotspot was identified emphasizing the variability within natural waters. This study provides the first comprehensive baseline concentrations of ARGs in the Danube River and lays the foundation for monitoring future trends and evaluating potential reduction measures. The applided holistic approach proved to be a valuable methodological contribution towards a better understanding of the environmental occurrence of AMR.",
publisher = "Elsevier",
journal = "Water Research",
title = "Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River",
volume = "252",
doi = "10.1016/j.watres.2024.121244",
pages = "121244"
}

Schachner-Gröhs, I., Koller, M., Leopold, M., Kolm, C., Linke, R., Jakwerth, S., Kolarević, S., Kračun-Kolarević, M., Kandler, W., Sulyok, M., Vierheilig, J., Toumi, M., Farkas, R., Toth, E., Kittinger, C., Zarfel, G., Farnleitner, A.,& Kirschner, A.. (2024). Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River. in Water Research
Elsevier., 252, 121244.
https://doi.org/10.1016/j.watres.2024.121244

Schachner-Gröhs I, Koller M, Leopold M, Kolm C, Linke R, Jakwerth S, Kolarević S, Kračun-Kolarević M, Kandler W, Sulyok M, Vierheilig J, Toumi M, Farkas R, Toth E, Kittinger C, Zarfel G, Farnleitner A, Kirschner A. Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River. in Water Research. 2024;252:121244.
doi:10.1016/j.watres.2024.121244 .

Schachner-Gröhs, Iris, Koller, Michael, Leopold, Melanie, Kolm, Claudia, Linke, Rita, Jakwerth, Stefan, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kandler, Wolfgang, Sulyok, Michael, Vierheilig, Julia, Toumi, Marwene, Farkas, Rózsa, Toth, Erika, Kittinger, Clemens, Zarfel, Gernot, Farnleitner, Andreas, Kirschner, Alexander, "Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River" in Water Research, 252 (2024):121244,
https://doi.org/10.1016/j.watres.2024.121244 . .

TY  - CONF
AU  - Farnleitner, Andreas
AU  - Schachner, Iris
AU  - Jakwerth, Stefan
AU  - Kittinger, Clemens
AU  - Zarfel, Gernot
AU  - Kavka, Gerhard
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Toth, Erika
AU  - Blaschke, Alfred
AU  - Derx, Julia
AU  - Linke, Rita
AU  - Demeter, Katalin
AU  - Savio, Domenico
AU  - Resicher, Georg
AU  - Kir, Alexander
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6596
AB  - Sustainable and target-oriented microbiological water quality management of rivers needs
information on whole river systems, especially if catchments are large and international. The
microbiological water quality of rivers is of uttermost relevance for human health as river water is
used for several purposes (recreation, drinking water production, irrigation). Besides information on
faecal pollution levels, the origin of faecal pollution and the assessment of associated infection- and
health risks, e g. from antimicrobial resistant bacteria (ARB) are of increasing importance. This
contribution summarizes the concepts and main results from the Joint-Danube-Surveys (JDS) and
discusses future challenges and perspectives (pollution-scenario modelling, infection-risk
assessment) for the Danube River concerning its faecal and AMR pollution status.
Between 2001 and 2019, the whole Danube was sampled four times during the JDS 1 to 4. Beside
standard faecal indicator analysis, cutting-edge molecular detection concepts were applied, including
microbial source tracking (MST) markers by quantitative PCR (qPCR) and high-throughput ampliconsequencing of bacterial communities. For AMR-profiling, clinically relevant bacterial species were
isolated and tested for resistances and resistance genes were determined via qPCR.
With this, we could impressively demonstrate that the JDS create the required multi-national “big
picture” of the microbiological pollution status of the Danube River. Harmonised trans-border
microbiological water quality maps for the whole navigable Danube were established. MST marker
analysis elucidated that the main faecal pollution source along the whole river is human wastewater.
Combined analysis of faecal indicators, MST and AMR-profiles provided a solid basis for assessing the
potential health impacts of AMR associated with faecal pollution
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance
SP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6596
ER  - 

@conference{
author = "Farnleitner, Andreas and Schachner, Iris and Jakwerth, Stefan and Kittinger, Clemens and Zarfel, Gernot and Kavka, Gerhard and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Toth, Erika and Blaschke, Alfred and Derx, Julia and Linke, Rita and Demeter, Katalin and Savio, Domenico and Resicher, Georg and Kir, Alexander",
year = "2023",
abstract = "Sustainable and target-oriented microbiological water quality management of rivers needs
information on whole river systems, especially if catchments are large and international. The
microbiological water quality of rivers is of uttermost relevance for human health as river water is
used for several purposes (recreation, drinking water production, irrigation). Besides information on
faecal pollution levels, the origin of faecal pollution and the assessment of associated infection- and
health risks, e g. from antimicrobial resistant bacteria (ARB) are of increasing importance. This
contribution summarizes the concepts and main results from the Joint-Danube-Surveys (JDS) and
discusses future challenges and perspectives (pollution-scenario modelling, infection-risk
assessment) for the Danube River concerning its faecal and AMR pollution status.
Between 2001 and 2019, the whole Danube was sampled four times during the JDS 1 to 4. Beside
standard faecal indicator analysis, cutting-edge molecular detection concepts were applied, including
microbial source tracking (MST) markers by quantitative PCR (qPCR) and high-throughput ampliconsequencing of bacterial communities. For AMR-profiling, clinically relevant bacterial species were
isolated and tested for resistances and resistance genes were determined via qPCR.
With this, we could impressively demonstrate that the JDS create the required multi-national “big
picture” of the microbiological pollution status of the Danube River. Harmonised trans-border
microbiological water quality maps for the whole navigable Danube were established. MST marker
analysis elucidated that the main faecal pollution source along the whole river is human wastewater.
Combined analysis of faecal indicators, MST and AMR-profiles provided a solid basis for assessing the
potential health impacts of AMR associated with faecal pollution",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance",
pages = "9",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6596"
}

Farnleitner, A., Schachner, I., Jakwerth, S., Kittinger, C., Zarfel, G., Kavka, G., Kolarević, S., Kračun-Kolarević, M., Toth, E., Blaschke, A., Derx, J., Linke, R., Demeter, K., Savio, D., Resicher, G.,& Kir, A.. (2023). Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 9.
https://hdl.handle.net/21.15107/rcub_ibiss_6596

Farnleitner A, Schachner I, Jakwerth S, Kittinger C, Zarfel G, Kavka G, Kolarević S, Kračun-Kolarević M, Toth E, Blaschke A, Derx J, Linke R, Demeter K, Savio D, Resicher G, Kir A. Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:9.
https://hdl.handle.net/21.15107/rcub_ibiss_6596 .

Farnleitner, Andreas, Schachner, Iris, Jakwerth, Stefan, Kittinger, Clemens, Zarfel, Gernot, Kavka, Gerhard, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Toth, Erika, Blaschke, Alfred, Derx, Julia, Linke, Rita, Demeter, Katalin, Savio, Domenico, Resicher, Georg, Kir, Alexander, "Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):9,
https://hdl.handle.net/21.15107/rcub_ibiss_6596 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Adrienn, Micsinai
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6591
AB  - Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6591
ER  - 

@conference{
author = "Kolarević, Stoimir and Adrienn, Micsinai and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2023",
abstract = "Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia",
pages = "11",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6591"
}

Kolarević, S., Adrienn, M., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2023). Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591

Kolarević S, Adrienn M, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

Kolarević, Stoimir, Adrienn, Micsinai, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):11,
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

TY  - CONF
AU  - Koller, Michel
AU  - Dielacher, Irina
AU  - Schachner, Iris
AU  - Leopold, Melanie
AU  - Jakwerth, Sefan
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Toth, Erika
AU  - Savio, Domenico
AU  - Farnleitner, Andreas
AU  - Kittinger, Clemens
AU  - Kirschner, Alexander
AU  - Zarfel, Gernot
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6593
AB  - Human induced antibiotic resistant bacteria (ARB) are not only found in clinical surroundings: large
rivers are of great concern as regards their spreading. This ongoing study´s aim is to analyse the
major propagation pathways and sources of ARB in the Danube, and to compare the results with
data obtained in 2013. Escherichia coli and Klebsiella spp. isolated during the 4th Joint Danube
Survey (2019) were tested for their antibiotic susceptibility. 22.24% of 1700 E. coli were resistant and
12.35% were multiresistant. 15.23 % of 696 Klebsiella spp. were resistant and 1.15% were
multiresistant. In comparison the data of 2013 and 2019 of both Enterobacteriaceae showed that the
wild type is still predominant. There were significant increases especially to fluoroquinolones and
augmentin and a significant decrease of resistances to tetracycline. However, the data of JDS4
showed a significant difference between E. coli and Klebsiella spp. regarding wild type, resistance and
multiresistance. Additionally, data of 1155 E. coli showed no seasonal trends in Austria during an
annual sampling. The comparison of E. coli from biofilms and corresponding water samples revealed
several significant differences between the compartments. In conclusion, the preliminary data of E.
coli from biofilm samples suggest that the biofilm as a stable colonization within waters reflects
especially the long term situation. Thus, the stabilizing and destabilizing effects acting on ARG within
the biofilm need to be researched in greater detail. Significant differences between the closely
related Enterobacteriaceae species seem to point out the dilemma of E. coli as a solely model
organism for environmental long term effects. Klebsiella spp. and their associated ARGs are facing a
different selective pressure when encountering the native environmental populations. Finally, the
results of the annual sampling campaign suggest that single environmental differences have a higher
impact than seasonal events.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6593
ER  - 

@conference{
author = "Koller, Michel and Dielacher, Irina and Schachner, Iris and Leopold, Melanie and Jakwerth, Sefan and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Toth, Erika and Savio, Domenico and Farnleitner, Andreas and Kittinger, Clemens and Kirschner, Alexander and Zarfel, Gernot",
year = "2023",
abstract = "Human induced antibiotic resistant bacteria (ARB) are not only found in clinical surroundings: large
rivers are of great concern as regards their spreading. This ongoing study´s aim is to analyse the
major propagation pathways and sources of ARB in the Danube, and to compare the results with
data obtained in 2013. Escherichia coli and Klebsiella spp. isolated during the 4th Joint Danube
Survey (2019) were tested for their antibiotic susceptibility. 22.24% of 1700 E. coli were resistant and
12.35% were multiresistant. 15.23 % of 696 Klebsiella spp. were resistant and 1.15% were
multiresistant. In comparison the data of 2013 and 2019 of both Enterobacteriaceae showed that the
wild type is still predominant. There were significant increases especially to fluoroquinolones and
augmentin and a significant decrease of resistances to tetracycline. However, the data of JDS4
showed a significant difference between E. coli and Klebsiella spp. regarding wild type, resistance and
multiresistance. Additionally, data of 1155 E. coli showed no seasonal trends in Austria during an
annual sampling. The comparison of E. coli from biofilms and corresponding water samples revealed
several significant differences between the compartments. In conclusion, the preliminary data of E.
coli from biofilm samples suggest that the biofilm as a stable colonization within waters reflects
especially the long term situation. Thus, the stabilizing and destabilizing effects acting on ARG within
the biofilm need to be researched in greater detail. Significant differences between the closely
related Enterobacteriaceae species seem to point out the dilemma of E. coli as a solely model
organism for environmental long term effects. Klebsiella spp. and their associated ARGs are facing a
different selective pressure when encountering the native environmental populations. Finally, the
results of the annual sampling campaign suggest that single environmental differences have a higher
impact than seasonal events.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6593"
}

Koller, M., Dielacher, I., Schachner, I., Leopold, M., Jakwerth, S., Kolarević, S., Kračun-Kolarević, M., Toth, E., Savio, D., Farnleitner, A., Kittinger, C., Kirschner, A.,& Zarfel, G.. (2023). The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_6593

Koller M, Dielacher I, Schachner I, Leopold M, Jakwerth S, Kolarević S, Kračun-Kolarević M, Toth E, Savio D, Farnleitner A, Kittinger C, Kirschner A, Zarfel G. The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_6593 .

Koller, Michel, Dielacher, Irina, Schachner, Iris, Leopold, Melanie, Jakwerth, Sefan, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Toth, Erika, Savio, Domenico, Farnleitner, Andreas, Kittinger, Clemens, Kirschner, Alexander, Zarfel, Gernot, "The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):24,
https://hdl.handle.net/21.15107/rcub_ibiss_6593 .

TY  - CONF
AU  - Schachner-Gröhs, Iris
AU  - Kolm, Claudia
AU  - Vierheilig, Julia
AU  - Leopold, Melanie
AU  - Zarfel, Gernot
AU  - Koller, Michael
AU  - Kittinger, Clemens
AU  - Jakwerth, Stefan
AU  - Linke, Rita
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Toth, Erika
AU  - Farnleitner, Andreas
AU  - Kirschner, Alexander
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6590
AB  - Antimicrobial resistance (AMR) represents one of the top ten global public health threats according
to the WHO. The spread of resistances is no longer limited to clinical settings and the natural
environment, especially aquatic ecosystems, are also strongly involved in the global spread. Holistic
as well as quantitative studies are still scarce. We here present for the first time a large scale study
on the Danube River regarding the distribution of AMR along different faecal pollution patterns.
For this study, samples from an international sampling campaign (Joint Danube Survey 4) as well as
additional seasonal samples were screened for the occurrence and origin of faecal pollution and for
the concentrations of nine representative antibiotic resistance genes (ARGs). In addition to water
samples also river biofilms were monitored to account for both, temporal and permanent river
habitats. Analyses were amended by a set of different cultivation-based and non-cultivation-based
microbiological and environmental data to explain the observed pattern in faecal pollution and ARG
concentrations.
Faecal pollution exists and varied strongly along the whole Danube river; hotspots could be identified
downstream of metropolitan areas at which also substantial concentrations of ARGs could be
detected. Quantifying concentrations of ARGs conferring resistance to different antibiotic classes
revealed strong differences between the different genes. Comparing their presence in water and
biofilm samples, allowed to assess how stably present these genes already are in the Danube River
ecosystem.
Environmental surveys are necessary to establish environmental baselines, to be able to guide future
AMR monitoring as well as to enforce useful management strategies. This integrative study therefore
provides first insights in the antimicrobial resistance situation in the most international river in the
world and second largest river in Europe
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Occurrence of antibiotic resistance genes along gradients of faecal pollution in water and biofilm samples from the whole Danube River
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6590
ER  - 

@conference{
author = "Schachner-Gröhs, Iris and Kolm, Claudia and Vierheilig, Julia and Leopold, Melanie and Zarfel, Gernot and Koller, Michael and Kittinger, Clemens and Jakwerth, Stefan and Linke, Rita and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Toth, Erika and Farnleitner, Andreas and Kirschner, Alexander",
year = "2023",
abstract = "Antimicrobial resistance (AMR) represents one of the top ten global public health threats according
to the WHO. The spread of resistances is no longer limited to clinical settings and the natural
environment, especially aquatic ecosystems, are also strongly involved in the global spread. Holistic
as well as quantitative studies are still scarce. We here present for the first time a large scale study
on the Danube River regarding the distribution of AMR along different faecal pollution patterns.
For this study, samples from an international sampling campaign (Joint Danube Survey 4) as well as
additional seasonal samples were screened for the occurrence and origin of faecal pollution and for
the concentrations of nine representative antibiotic resistance genes (ARGs). In addition to water
samples also river biofilms were monitored to account for both, temporal and permanent river
habitats. Analyses were amended by a set of different cultivation-based and non-cultivation-based
microbiological and environmental data to explain the observed pattern in faecal pollution and ARG
concentrations.
Faecal pollution exists and varied strongly along the whole Danube river; hotspots could be identified
downstream of metropolitan areas at which also substantial concentrations of ARGs could be
detected. Quantifying concentrations of ARGs conferring resistance to different antibiotic classes
revealed strong differences between the different genes. Comparing their presence in water and
biofilm samples, allowed to assess how stably present these genes already are in the Danube River
ecosystem.
Environmental surveys are necessary to establish environmental baselines, to be able to guide future
AMR monitoring as well as to enforce useful management strategies. This integrative study therefore
provides first insights in the antimicrobial resistance situation in the most international river in the
world and second largest river in Europe",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Occurrence of antibiotic resistance genes along gradients of faecal pollution in water and biofilm samples from the whole Danube River",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6590"
}

Schachner-Gröhs, I., Kolm, C., Vierheilig, J., Leopold, M., Zarfel, G., Koller, M., Kittinger, C., Jakwerth, S., Linke, R., Kolarević, S., Kračun-Kolarević, M., Toth, E., Farnleitner, A.,& Kirschner, A.. (2023). Occurrence of antibiotic resistance genes along gradients of faecal pollution in water and biofilm samples from the whole Danube River. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 25.
https://hdl.handle.net/21.15107/rcub_ibiss_6590

Schachner-Gröhs I, Kolm C, Vierheilig J, Leopold M, Zarfel G, Koller M, Kittinger C, Jakwerth S, Linke R, Kolarević S, Kračun-Kolarević M, Toth E, Farnleitner A, Kirschner A. Occurrence of antibiotic resistance genes along gradients of faecal pollution in water and biofilm samples from the whole Danube River. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:25.
https://hdl.handle.net/21.15107/rcub_ibiss_6590 .

Schachner-Gröhs, Iris, Kolm, Claudia, Vierheilig, Julia, Leopold, Melanie, Zarfel, Gernot, Koller, Michael, Kittinger, Clemens, Jakwerth, Stefan, Linke, Rita, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Toth, Erika, Farnleitner, Andreas, Kirschner, Alexander, "Occurrence of antibiotic resistance genes along gradients of faecal pollution in water and biofilm samples from the whole Danube River" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):25,
https://hdl.handle.net/21.15107/rcub_ibiss_6590 .

TY  - CHAP
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović, Jovana
AU  - Ilić, Marija
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Martinović, Rajko
AU  - Jokanović, Sandra
AU  - Joksimović, Danijela
AU  - Pešić, Vladimir
AU  - Kirschner, Alexander K. T.
AU  - Linke, Rita
AU  - Ixenmaier, Simone
AU  - Farnleitner, Andreas
AU  - Savio, Domenico
AU  - Reischer, Georg
AU  - Tomić, Nevenka
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - https://link.springer.com/chapter/10.1007/698_2019_420
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3952
AB  - The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.
PB  - Springer
T2  - The Rivers of Montenegro
T2  - The Rivers of Montenegro
T1  - Microbiological Water Quality of Rivers in Montenegro
DO  - 10.1007/698_2019_420
SP  - 135
EP  - 155
ER  - 

@inbook{
editor = "Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G.",
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović, Jovana and Ilić, Marija and Paunović, Momir and Kostić-Vuković, Jovana and Martinović, Rajko and Jokanović, Sandra and Joksimović, Danijela and Pešić, Vladimir and Kirschner, Alexander K. T. and Linke, Rita and Ixenmaier, Simone and Farnleitner, Andreas and Savio, Domenico and Reischer, Georg and Tomić, Nevenka and Vuković-Gačić, Branka",
year = "2020",
abstract = "The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.",
publisher = "Springer",
journal = "The Rivers of Montenegro, The Rivers of Montenegro",
booktitle = "Microbiological Water Quality of Rivers in Montenegro",
doi = "10.1007/698_2019_420",
pages = "135-155"
}

Pešić, V., Paunović, M., Kostianoy, A. G., Kolarević, S., Kračun-Kolarević, M., Jovanović, J., Ilić, M., Paunović, M., Kostić-Vuković, J., Martinović, R., Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A. K. T., Linke, R., Ixenmaier, S., Farnleitner, A., Savio, D., Reischer, G., Tomić, N.,& Vuković-Gačić, B.. (2020). Microbiological Water Quality of Rivers in Montenegro. in The Rivers of Montenegro
Springer., 135-155.
https://doi.org/10.1007/698_2019_420

Pešić V, Paunović M, Kostianoy AG, Kolarević S, Kračun-Kolarević M, Jovanović J, Ilić M, Paunović M, Kostić-Vuković J, Martinović R, Jokanović S, Joksimović D, Pešić V, Kirschner AKT, Linke R, Ixenmaier S, Farnleitner A, Savio D, Reischer G, Tomić N, Vuković-Gačić B. Microbiological Water Quality of Rivers in Montenegro. in The Rivers of Montenegro. 2020;:135-155.
doi:10.1007/698_2019_420 .

Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G., Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović, Jovana, Ilić, Marija, Paunović, Momir, Kostić-Vuković, Jovana, Martinović, Rajko, Jokanović, Sandra, Joksimović, Danijela, Pešić, Vladimir, Kirschner, Alexander K. T., Linke, Rita, Ixenmaier, Simone, Farnleitner, Andreas, Savio, Domenico, Reischer, Georg, Tomić, Nevenka, Vuković-Gačić, Branka, "Microbiological Water Quality of Rivers in Montenegro" in The Rivers of Montenegro (2020):135-155,
https://doi.org/10.1007/698_2019_420 . .

TY  - JOUR
AU  - Vrzel, Janja
AU  - Vuković-Gačić, Branka
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kračun-Kolarević, Margareta
AU  - Kostić, Jovana
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Ogrinc, Nives
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0048969716316667
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84994378206&origin=SingleRecordEmailAlert&txGid=6CE299281CDB840158BFAC52EC5A2E1C.wsnAw8kcdt7IPYLO0V48gA:71#
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2908
AB  - Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (δ15NNO3 and δ18ONO3) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.
T2  - Science of The Total Environment
T2  - Science of The Total Environment
T1  - Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.213
SP  - 1460
EP  - 1471
ER  - 

@article{
author = "Vrzel, Janja and Vuković-Gačić, Branka and Kolarević, Stoimir and Gačić, Zoran and Kračun-Kolarević, Margareta and Kostić, Jovana and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Ogrinc, Nives",
year = "2016",
abstract = "Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (δ15NNO3 and δ18ONO3) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.",
journal = "Science of The Total Environment, Science of The Total Environment",
title = "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.213",
pages = "1460-1471"
}

Vrzel, J., Vuković-Gačić, B., Kolarević, S., Gačić, Z., Kračun-Kolarević, M., Kostić, J., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Ogrinc, N.. (2016). Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of The Total Environment, 573, 1460-1471.
https://doi.org/10.1016/j.scitotenv.2016.07.213

Vrzel J, Vuković-Gačić B, Kolarević S, Gačić Z, Kračun-Kolarević M, Kostić J, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Ogrinc N. Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of The Total Environment. 2016;573:1460-1471.
doi:10.1016/j.scitotenv.2016.07.213 .

Vrzel, Janja, Vuković-Gačić, Branka, Kolarević, Stoimir, Gačić, Zoran, Kračun-Kolarević, Margareta, Kostić, Jovana, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Ogrinc, Nives, "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin" in Science of The Total Environment, 573 (2016):1460-1471,
https://doi.org/10.1016/j.scitotenv.2016.07.213 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kračun-Kolarević, Margareta
AU  - Kostić, Jovana
AU  - Simonović, Predrag
AU  - Simić, Vladica
AU  - Milošković, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milačič, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://dx.plos.org/10.1371/journal.pone.0162450
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84992314127&origin=SingleRecordEmailAlert&txGid=06CA159BD204EA9525A7DB2D67E8F57F.wsnAw8kcdt7IPYLO0V48gA:19
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2503
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimuriumTA1535/ pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/ Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrialwastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
T2  - Plos ONE
T1  - Evaluation of Genotoxic Pressure along the Sava River
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0162450
SP  - e0162450
EP  - e0162450
ER  - 

@article{
author = "Kolarević, Stoimir and Aborgiba, Mustafa and Kračun-Kolarević, Margareta and Kostić, Jovana and Simonović, Predrag and Simić, Vladica and Milošković, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milačič, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vuković-Gačić, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimuriumTA1535/ pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/ Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrialwastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
journal = "Plos ONE",
title = "Evaluation of Genotoxic Pressure along the Sava River",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0162450",
pages = "e0162450-e0162450"
}

Kolarević, S., Aborgiba, M., Kračun-Kolarević, M., Kostić, J., Simonović, P., Simić, V., Milošković, A., Reischer, G., Farnleitner, A., Gačić, Z., Milačič, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vuković-Gačić, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in Plos ONE, 11(9), e0162450-e0162450.
https://doi.org/10.1371/journal.pone.0162450

Kolarević S, Aborgiba M, Kračun-Kolarević M, Kostić J, Simonović P, Simić V, Milošković A, Reischer G, Farnleitner A, Gačić Z, Milačič R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vuković-Gačić B. Evaluation of Genotoxic Pressure along the Sava River. in Plos ONE. 2016;11(9):e0162450-e0162450.
doi:10.1371/journal.pone.0162450 .

Kolarević, Stoimir, Aborgiba, Mustafa, Kračun-Kolarević, Margareta, Kostić, Jovana, Simonović, Predrag, Simić, Vladica, Milošković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milačič, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vuković-Gačić, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in Plos ONE, 11, no. 9 (2016):e0162450-e0162450,
https://doi.org/10.1371/journal.pone.0162450 . .