TY  - JOUR
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Uskoković, Aleksandra
AU  - Stanković, Vesna
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Solujić, Slavica
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
PY  - 2014
UR  - http://www.ncbi.nlm.nih.gov/pubmed/24468630
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3185
AB  - Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.
PB  - Elsevier
T2  - European Journal of Pharmaceutical Sciences
T1  - Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.
IS  - 1
VL  - 55
DO  - 10.1016/j.ejps.2014.01.004
SP  - 20
EP  - 35
ER  - 

@article{
author = "Stanković, Nevena and Mladenović, Milan and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Uskoković, Aleksandra and Stanković, Vesna and Mihailović, Vladimir and Katanić, Jelena and Matić, Sanja and Solujić, Slavica and Vuković, Nenad and Sukdolak, Slobodan",
year = "2014",
abstract = "Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.",
publisher = "Elsevier",
journal = "European Journal of Pharmaceutical Sciences",
title = "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.",
number = "1",
volume = "55",
doi = "10.1016/j.ejps.2014.01.004",
pages = "20-35"
}

Stanković, N., Mladenović, M., Mihailović, M., Arambašić Jovanović, J., Uskoković, A., Stanković, V., Mihailović, V., Katanić, J., Matić, S., Solujić, S., Vuković, N.,& Sukdolak, S.. (2014). Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences
Elsevier., 55(1), 20-35.
https://doi.org/10.1016/j.ejps.2014.01.004

Stanković N, Mladenović M, Mihailović M, Arambašić Jovanović J, Uskoković A, Stanković V, Mihailović V, Katanić J, Matić S, Solujić S, Vuković N, Sukdolak S. Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences. 2014;55(1):20-35.
doi:10.1016/j.ejps.2014.01.004 .

Stanković, Nevena, Mladenović, Milan, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Uskoković, Aleksandra, Stanković, Vesna, Mihailović, Vladimir, Katanić, Jelena, Matić, Sanja, Solujić, Slavica, Vuković, Nenad, Sukdolak, Slobodan, "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model." in European Journal of Pharmaceutical Sciences, 55, no. 1 (2014):20-35,
https://doi.org/10.1016/j.ejps.2014.01.004 . .

TY  - CONF
AU  - Mihailović, Vladimir
AU  - Mišić, Danijela
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
AU  - Solujić, Slavica
AU  - Stanković, Vesna
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6142
AB  - Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was perform­ed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependent­ly and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower.
PB  - Belgrade: Serbian Chemical Society
C3  - Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia
T1  - Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats
SP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6142
ER  - 

@conference{
author = "Mihailović, Vladimir and Mišić, Danijela and Katanić, Jelena and Mihailović, Mirjana and Solujić, Slavica and Stanković, Vesna and Mladenović, Milan and Stanković, Nevena",
year = "2013",
abstract = "Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was perform­ed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependent­ly and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia",
title = "Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats",
pages = "220",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6142"
}

Mihailović, V., Mišić, D., Katanić, J., Mihailović, M., Solujić, S., Stanković, V., Mladenović, M.,& Stanković, N.. (2013). Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats. in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia
Belgrade: Serbian Chemical Society., 220.
https://hdl.handle.net/21.15107/rcub_ibiss_6142

Mihailović V, Mišić D, Katanić J, Mihailović M, Solujić S, Stanković V, Mladenović M, Stanković N. Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats. in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia. 2013;:220.
https://hdl.handle.net/21.15107/rcub_ibiss_6142 .

Mihailović, Vladimir, Mišić, Danijela, Katanić, Jelena, Mihailović, Mirjana, Solujić, Slavica, Stanković, Vesna, Mladenović, Milan, Stanković, Nevena, "Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats" in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia (2013):220,
https://hdl.handle.net/21.15107/rcub_ibiss_6142 .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://www.sciencedirect.com/science/article/pii/S1383571813001836
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3188
AB  - The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.
PB  - Elsevier
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury
IS  - 2
VL  - 755
DO  - 10.1016/j.mrgentox.2013.03.011
SP  - 81
EP  - 89
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Vidaković, Melita and Grdović, Nevena and Dinić, Svetlana and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Mirjana",
year = "2013",
abstract = "The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.",
publisher = "Elsevier",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury",
number = "2",
volume = "755",
doi = "10.1016/j.mrgentox.2013.03.011",
pages = "81-89"
}

Matić, S., Stanić, S., Bogojević, D., Vidaković, M., Grdović, N., Dinić, S., Solujić, S., Mladenović, M., Stanković, N.,& Mihailović, M.. (2013). Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier., 755(2), 81-89.
https://doi.org/10.1016/j.mrgentox.2013.03.011

Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Dinić S, Solujić S, Mladenović M, Stanković N, Mihailović M. Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2013;755(2):81-89.
doi:10.1016/j.mrgentox.2013.03.011 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Vidaković, Melita, Grdović, Nevena, Dinić, Svetlana, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Mirjana, "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 755, no. 2 (2013):81-89,
https://doi.org/10.1016/j.mrgentox.2013.03.011 . .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Mihailović, Mirjana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Katanić, Jelena
AU  - Mladenović, Milan
AU  - Solujić, Slavica
AU  - Matić, Sanja
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1043
AB  - This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GM) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GM and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GM treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity. (C) 2012 Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats
VL  - 52
DO  - 10.1016/j.fct.2012.10.034
SP  - 83
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1043
ER  - 

@article{
author = "Mihailović, Vladimir and Mihailović, Mirjana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Mišić, Danijela and Stanković, Vesna and Katanić, Jelena and Mladenović, Milan and Solujić, Slavica and Matić, Sanja",
year = "2013",
abstract = "This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GM) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GM and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GM treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity. (C) 2012 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats",
volume = "52",
doi = "10.1016/j.fct.2012.10.034",
pages = "83-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1043"
}

Mihailović, V., Mihailović, M., Uskoković, A., Arambašić Jovanović, J., Mišić, D., Stanković, V., Katanić, J., Mladenović, M., Solujić, S.,& Matić, S.. (2013). Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats. in Food and Chemical Toxicology
Elsevier., 52, 83-90.
https://doi.org/10.1016/j.fct.2012.10.034
https://hdl.handle.net/21.15107/rcub_ibiss_1043

Mihailović V, Mihailović M, Uskoković A, Arambašić Jovanović J, Mišić D, Stanković V, Katanić J, Mladenović M, Solujić S, Matić S. Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats. in Food and Chemical Toxicology. 2013;52:83-90.
doi:10.1016/j.fct.2012.10.034
https://hdl.handle.net/21.15107/rcub_ibiss_1043 .

Mihailović, Vladimir, Mihailović, Mirjana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Mišić, Danijela, Stanković, Vesna, Katanić, Jelena, Mladenović, Milan, Solujić, Slavica, Matić, Sanja, "Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats" in Food and Chemical Toxicology, 52 (2013):83-90,
https://doi.org/10.1016/j.fct.2012.10.034 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1043 .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Mišić, Danijela
AU  - Solujić, Slavica R
AU  - Stanić, Snezana M
AU  - Katanić, Jelena
AU  - Mladenović, Milan P
AU  - Stanković, Nevena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1049
AB  - The aim of this study was to evaluate the antioxidant and antigenotoxic activities of chloroform, ethyl acetate and n-butanol fractions obtained from Gentiana asclepiadea L. roots methanolic extract. The main secondary metabolites sweroside, swertiamarin and gentiopicrine were quantified in G. asclepiadea root extracts using HPLC-DAD analysis. Amount of total phenols, flavonoids, flavonols and gallotannins was also determined. The antigenotoxic potential of extracts from roots of G. asclepiadea was assessed using the standard in vivo procedure for the detection of sex linked recessive lethal mutations in Drosophila melanogaster males treated with ethyl methanesulfonate (EMS). The results showed that the most abundant secoiridoid in G. asclepiadea roots was gentiopicrine and its content in the n-butanol fraction (442.89 mg/g) was the highest. Among all extracts, ethyl acetate fraction showed the highest antioxidant activity, as well as total phenolics (146.64 GAE/g), flavonoids (44.62 RUE/g), flavonols (22.71 RUE/g) and gallotannins (0.99 mg GAE/g) content. All the fractions showed antioxidant activity using in vitro model systems and the results have been correlated with total phenolics, flavonoids, flavonols and gallotannins content. In addition to antioxidant activity, G. asclepiadea root extract fractions possess an antigenotoxic effect against DNA damage induced by alkylation with EMS. The antioxidant activity exhibited by G. asclepiadea depended on the phenolic compounds content of the tested extracts, while there was no significant difference in the antigenotoxic potential between fractions.
T2  - Excli Journal
T1  - Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract
IS  - null
VL  - 12
SP  - 373
EP  - 823
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1049
ER  - 

@article{
author = "Mihailović, Vladimir and Matić, Sanja and Mišić, Danijela and Solujić, Slavica R and Stanić, Snezana M and Katanić, Jelena and Mladenović, Milan P and Stanković, Nevena",
year = "2013",
abstract = "The aim of this study was to evaluate the antioxidant and antigenotoxic activities of chloroform, ethyl acetate and n-butanol fractions obtained from Gentiana asclepiadea L. roots methanolic extract. The main secondary metabolites sweroside, swertiamarin and gentiopicrine were quantified in G. asclepiadea root extracts using HPLC-DAD analysis. Amount of total phenols, flavonoids, flavonols and gallotannins was also determined. The antigenotoxic potential of extracts from roots of G. asclepiadea was assessed using the standard in vivo procedure for the detection of sex linked recessive lethal mutations in Drosophila melanogaster males treated with ethyl methanesulfonate (EMS). The results showed that the most abundant secoiridoid in G. asclepiadea roots was gentiopicrine and its content in the n-butanol fraction (442.89 mg/g) was the highest. Among all extracts, ethyl acetate fraction showed the highest antioxidant activity, as well as total phenolics (146.64 GAE/g), flavonoids (44.62 RUE/g), flavonols (22.71 RUE/g) and gallotannins (0.99 mg GAE/g) content. All the fractions showed antioxidant activity using in vitro model systems and the results have been correlated with total phenolics, flavonoids, flavonols and gallotannins content. In addition to antioxidant activity, G. asclepiadea root extract fractions possess an antigenotoxic effect against DNA damage induced by alkylation with EMS. The antioxidant activity exhibited by G. asclepiadea depended on the phenolic compounds content of the tested extracts, while there was no significant difference in the antigenotoxic potential between fractions.",
journal = "Excli Journal",
title = "Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract",
number = "null",
volume = "12",
pages = "373-823",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1049"
}

Mihailović, V., Matić, S., Mišić, D., Solujić, S. R., Stanić, S. M., Katanić, J., Mladenović, M. P.,& Stanković, N.. (2013). Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract. in Excli Journal, 12(null), 373-823.
https://hdl.handle.net/21.15107/rcub_ibiss_1049

Mihailović V, Matić S, Mišić D, Solujić SR, Stanić SM, Katanić J, Mladenović MP, Stanković N. Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract. in Excli Journal. 2013;12(null):373-823.
https://hdl.handle.net/21.15107/rcub_ibiss_1049 .

Mihailović, Vladimir, Matić, Sanja, Mišić, Danijela, Solujić, Slavica R, Stanić, Snezana M, Katanić, Jelena, Mladenović, Milan P, Stanković, Nevena, "Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract" in Excli Journal, 12, no. null (2013):373-823,
https://hdl.handle.net/21.15107/rcub_ibiss_1049 .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Mašković, Pavle
AU  - Vrvić, Miroslav M.
AU  - Solujić, Slavica
PY  - 2012
UR  - https://www.sciencedirect.com/science/article/pii/S0223523412002887?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3201
AB  - The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.
T2  - European Journal of Medicinal Chemistry
T1  - Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants
VL  - 54
DO  - 10.1016/J.EJMECH.2012.04.036
SP  - 144
EP  - 158
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Vuković, Nenad and Sukdolak, Slobodan and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Mašković, Pavle and Vrvić, Miroslav M. and Solujić, Slavica",
year = "2012",
abstract = "The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.",
journal = "European Journal of Medicinal Chemistry",
title = "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants",
volume = "54",
doi = "10.1016/J.EJMECH.2012.04.036",
pages = "144-158"
}

Mladenović, M., Mihailović, M., Bogojević, D., Vuković, N., Sukdolak, S., Matić, S., Nićiforović, N., Mihailović, V., Mašković, P., Vrvić, M. M.,& Solujić, S.. (2012). Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry, 54, 144-158.
https://doi.org/10.1016/J.EJMECH.2012.04.036

Mladenović M, Mihailović M, Bogojević D, Vuković N, Sukdolak S, Matić S, Nićiforović N, Mihailović V, Mašković P, Vrvić MM, Solujić S. Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry. 2012;54:144-158.
doi:10.1016/J.EJMECH.2012.04.036 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Vuković, Nenad, Sukdolak, Slobodan, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Mašković, Pavle, Vrvić, Miroslav M., Solujić, Slavica, "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants" in European Journal of Medicinal Chemistry, 54 (2012):144-158,
https://doi.org/10.1016/J.EJMECH.2012.04.036 . .

TY  - JOUR
AU  - Mašković, Pavle
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Manojlović, Nedeljko T.
AU  - Radojković, Marija
AU  - Cvijović, Milica
AU  - Solujić, Slavica
PY  - 2012
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4675
AB  - The objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H. sendtneri was   exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H.  sendtneri.
PB  - Warsaw: Versita
T2  - Central European Journal of Biology
T1  - Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler
IS  - 2
VL  - 7
DO  - 10.2478/s11535-012-0021-8
SP  - 327
EP  - 333
ER  - 

@article{
author = "Mašković, Pavle and Dragišić Maksimović, Jelena and Maksimović, Vuk and Blagojević, Jelena and Vujošević, Mladen and Manojlović, Nedeljko T. and Radojković, Marija and Cvijović, Milica and Solujić, Slavica",
year = "2012",
abstract = "The objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H. sendtneri was   exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H.  sendtneri.",
publisher = "Warsaw: Versita",
journal = "Central European Journal of Biology",
title = "Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler",
number = "2",
volume = "7",
doi = "10.2478/s11535-012-0021-8",
pages = "327-333"
}

Mašković, P., Dragišić Maksimović, J., Maksimović, V., Blagojević, J., Vujošević, M., Manojlović, N. T., Radojković, M., Cvijović, M.,& Solujić, S.. (2012). Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler. in Central European Journal of Biology
Warsaw: Versita., 7(2), 327-333.
https://doi.org/10.2478/s11535-012-0021-8

Mašković P, Dragišić Maksimović J, Maksimović V, Blagojević J, Vujošević M, Manojlović NT, Radojković M, Cvijović M, Solujić S. Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler. in Central European Journal of Biology. 2012;7(2):327-333.
doi:10.2478/s11535-012-0021-8 .

Mašković, Pavle, Dragišić Maksimović, Jelena, Maksimović, Vuk, Blagojević, Jelena, Vujošević, Mladen, Manojlović, Nedeljko T., Radojković, Marija, Cvijović, Milica, Solujić, Slavica, "Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri(Boiss) Dőrfler" in Central European Journal of Biology, 7, no. 2 (2012):327-333,
https://doi.org/10.2478/s11535-012-0021-8 . .

TY  - JOUR
AU  - Stanić, Snežana
AU  - Matić, Sanja
AU  - Đelić, Gorica
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Solujić, Slavica
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3208
AB  - The genotoxic and antigenotoxic effects of Cotinus coggygria Scop. methanol extract was investigated using the Drosophila sex-linked recessive lethal (or SLRL) test. The results presented here show that the methanol extract of Cotinus coggygria in a concentration of 5% and artificial chemical agent ethyl methanesulfonate EMS (0.75 ppm) induce recessive lethal mutations on X-chromosome on Drosophila melanogaster in all broods (I, II and III). Post-treatment with lower concentration of the methanol extract of Cotinus coggygria (2%) was effective in reducing genotoxicity ofmutagen.
PB  - Springer Nature
T2  - Russian Journal of Genetics
T1  - Study of genotoxicity and antigenotoxicity of the Cotinus coggygria Scop. methanol extract by Drosophila melanogaster sex-linked recessive lethal test.
IS  - 7
VL  - 47
DO  - 10.1134/S1022795411070167
SP  - 770
EP  - 774
ER  - 

@article{
author = "Stanić, Snežana and Matić, Sanja and Đelić, Gorica and Mihailović, Mirjana and Bogojević, Desanka and Solujić, Slavica",
year = "2011",
abstract = "The genotoxic and antigenotoxic effects of Cotinus coggygria Scop. methanol extract was investigated using the Drosophila sex-linked recessive lethal (or SLRL) test. The results presented here show that the methanol extract of Cotinus coggygria in a concentration of 5% and artificial chemical agent ethyl methanesulfonate EMS (0.75 ppm) induce recessive lethal mutations on X-chromosome on Drosophila melanogaster in all broods (I, II and III). Post-treatment with lower concentration of the methanol extract of Cotinus coggygria (2%) was effective in reducing genotoxicity ofmutagen.",
publisher = "Springer Nature",
journal = "Russian Journal of Genetics",
title = "Study of genotoxicity and antigenotoxicity of the Cotinus coggygria Scop. methanol extract by Drosophila melanogaster sex-linked recessive lethal test.",
number = "7",
volume = "47",
doi = "10.1134/S1022795411070167",
pages = "770-774"
}

Stanić, S., Matić, S., Đelić, G., Mihailović, M., Bogojević, D.,& Solujić, S.. (2011). Study of genotoxicity and antigenotoxicity of the Cotinus coggygria Scop. methanol extract by Drosophila melanogaster sex-linked recessive lethal test.. in Russian Journal of Genetics
Springer Nature., 47(7), 770-774.
https://doi.org/10.1134/S1022795411070167

Stanić S, Matić S, Đelić G, Mihailović M, Bogojević D, Solujić S. Study of genotoxicity and antigenotoxicity of the Cotinus coggygria Scop. methanol extract by Drosophila melanogaster sex-linked recessive lethal test.. in Russian Journal of Genetics. 2011;47(7):770-774.
doi:10.1134/S1022795411070167 .

Stanić, Snežana, Matić, Sanja, Đelić, Gorica, Mihailović, Mirjana, Bogojević, Desanka, Solujić, Slavica, "Study of genotoxicity and antigenotoxicity of the Cotinus coggygria Scop. methanol extract by Drosophila melanogaster sex-linked recessive lethal test." in Russian Journal of Genetics, 47, no. 7 (2011):770-774,
https://doi.org/10.1134/S1022795411070167 . .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snećana
AU  - Bogojević, Desanka
AU  - Solujić, Slavica
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Mihailović, Mirjana
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3205
AB  - The intention was to evaluate the possible in vivo genotoxic potential in different cell-types, of a methanol extract obtained from the plant stem of Cotinus coggygria Scop., using the sex-linked recessive lethal (or SLRL) test and alkaline comet assay. The SLRL test, revealed the genotoxic effect of this extract in postmeiotic and premeiotic germ-cell lines. The comet assay was carried out on rat liver and bone marrow at 24 and 72 h after intraperitoneal administration. For genotoxic evaluation, three concentrations of the extract were tested, viz., 500, 1000 and 2000 mg/kg body weight (bw), based on the solubility limit of the extract in saline. Comet tail moment and total scores in the group treated with 500 mg/kg bw, 24 and 72 h after treatment, were not significantly different from the control group, whereas in the groups of animals, under the same conditions, but with 1000 and 2000 mg/kg bw of the extract, scores were statistically so. A slight decrease in the comet score and tail moment observed in all the doses in the 72 h treatment, gave to understand that DNA damage induced by Cotinus coggygria extract decreased with time. The results of both tests revealed the genotoxic effect of Cotinus coggygria under our experimental conditions.
PB  - Sociedade Brasileira de Genética
T2  - Genetics and Molecular Biology
T1  - Genotoxic potential of Cotinus coggygriascop. (Anacardiaceae) stem extract in vivo
IS  - 2
VL  - 34
DO  - 10.1590/S1415-47572011005000001
SP  - 298
EP  - 303
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snećana and Bogojević, Desanka and Solujić, Slavica and Grdović, Nevena and Vidaković, Melita and Mihailović, Mirjana",
year = "2011",
abstract = "The intention was to evaluate the possible in vivo genotoxic potential in different cell-types, of a methanol extract obtained from the plant stem of Cotinus coggygria Scop., using the sex-linked recessive lethal (or SLRL) test and alkaline comet assay. The SLRL test, revealed the genotoxic effect of this extract in postmeiotic and premeiotic germ-cell lines. The comet assay was carried out on rat liver and bone marrow at 24 and 72 h after intraperitoneal administration. For genotoxic evaluation, three concentrations of the extract were tested, viz., 500, 1000 and 2000 mg/kg body weight (bw), based on the solubility limit of the extract in saline. Comet tail moment and total scores in the group treated with 500 mg/kg bw, 24 and 72 h after treatment, were not significantly different from the control group, whereas in the groups of animals, under the same conditions, but with 1000 and 2000 mg/kg bw of the extract, scores were statistically so. A slight decrease in the comet score and tail moment observed in all the doses in the 72 h treatment, gave to understand that DNA damage induced by Cotinus coggygria extract decreased with time. The results of both tests revealed the genotoxic effect of Cotinus coggygria under our experimental conditions.",
publisher = "Sociedade Brasileira de Genética",
journal = "Genetics and Molecular Biology",
title = "Genotoxic potential of Cotinus coggygriascop. (Anacardiaceae) stem extract in vivo",
number = "2",
volume = "34",
doi = "10.1590/S1415-47572011005000001",
pages = "298-303"
}

Matić, S., Stanić, S., Bogojević, D., Solujić, S., Grdović, N., Vidaković, M.,& Mihailović, M.. (2011). Genotoxic potential of Cotinus coggygriascop. (Anacardiaceae) stem extract in vivo. in Genetics and Molecular Biology
Sociedade Brasileira de Genética., 34(2), 298-303.
https://doi.org/10.1590/S1415-47572011005000001

Matić S, Stanić S, Bogojević D, Solujić S, Grdović N, Vidaković M, Mihailović M. Genotoxic potential of Cotinus coggygriascop. (Anacardiaceae) stem extract in vivo. in Genetics and Molecular Biology. 2011;34(2):298-303.
doi:10.1590/S1415-47572011005000001 .

Matić, Sanja, Stanić, Snećana, Bogojević, Desanka, Solujić, Slavica, Grdović, Nevena, Vidaković, Melita, Mihailović, Mirjana, "Genotoxic potential of Cotinus coggygriascop. (Anacardiaceae) stem extract in vivo" in Genetics and Molecular Biology, 34, no. 2 (2011):298-303,
https://doi.org/10.1590/S1415-47572011005000001 . .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Arambašić Jovanović, Jelena
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Poznanović, Goran
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Marković, Jelena
AU  - Mihailović, Mirjana
PY  - 2011
UR  - http://www.nrcresearchpress.com/doi/10.1139/y11-043#.XBD8lM0o-Uk
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3204
AB  - To examine the protective potential of the Cotinus coggygria Scop. methanol extract, Wistar rats were treated with the hepatotoxic compound pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The ability of the extract to counteract the oxidative stress was examined in rats that were injected with the extract intraperitoneally (500 mg·(kg body weight)(-1)) either 2 or 12 h before the pyrogallol treatment. The extract possesses a reducing activity in vitro and an ability to chelate the ferrous ion both in vivo and in vitro. Application of the extract prior to pyrogallol treatment led to a decrease in the levels of thiobarbituric acid-reactive substances, aspartate aminotransferase, and alanine aminotransferase, increased activities of antioxidant enzymes and attenuation of DNA damage, as well as increased Akt activity and inhibition of NF-κB protein expression. Treatment with the extract 12 h prior to pyrogallol administration was more effective in suppressing pyrogallol-induced oxidative damage than the 2 h pretreatment. Extract administration promoted an increase in acute phase reactants haptoglobin and α(2)-macroglobulin that was short of a full-fledged acute phase response. Administration of the extract considerably improved the markers of oxidative stress, thus revealing a potential hepatoprotective activity. Our results suggest that Akt activation, NF-κB inhibition, and induction of the acute phase play important roles in mediating hepatic protection by the extract. The greater effectiveness of the 12 h pretreatment with extract points to the important role that preconditioning assumes in improving resistance to subsequent exposure to oxidative stress.
PB  - NRC Research Press; Canadian Science Publishing; National Research Council of Canada
PB  - © 2011 by NRC Research Press
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats
IS  - 6
VL  - 89
DO  - 10.1139/y11-043
SP  - 401
EP  - 411
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Vidaković, Melita and Grdović, Nevena and Arambašić Jovanović, Jelena and Dinić, Svetlana and Uskoković, Aleksandra and Poznanović, Goran and Solujić, Slavica and Mladenović, Milan and Marković, Jelena and Mihailović, Mirjana",
year = "2011",
abstract = "To examine the protective potential of the Cotinus coggygria Scop. methanol extract, Wistar rats were treated with the hepatotoxic compound pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The ability of the extract to counteract the oxidative stress was examined in rats that were injected with the extract intraperitoneally (500 mg·(kg body weight)(-1)) either 2 or 12 h before the pyrogallol treatment. The extract possesses a reducing activity in vitro and an ability to chelate the ferrous ion both in vivo and in vitro. Application of the extract prior to pyrogallol treatment led to a decrease in the levels of thiobarbituric acid-reactive substances, aspartate aminotransferase, and alanine aminotransferase, increased activities of antioxidant enzymes and attenuation of DNA damage, as well as increased Akt activity and inhibition of NF-κB protein expression. Treatment with the extract 12 h prior to pyrogallol administration was more effective in suppressing pyrogallol-induced oxidative damage than the 2 h pretreatment. Extract administration promoted an increase in acute phase reactants haptoglobin and α(2)-macroglobulin that was short of a full-fledged acute phase response. Administration of the extract considerably improved the markers of oxidative stress, thus revealing a potential hepatoprotective activity. Our results suggest that Akt activation, NF-κB inhibition, and induction of the acute phase play important roles in mediating hepatic protection by the extract. The greater effectiveness of the 12 h pretreatment with extract points to the important role that preconditioning assumes in improving resistance to subsequent exposure to oxidative stress.",
publisher = "NRC Research Press; Canadian Science Publishing; National Research Council of Canada, © 2011 by NRC Research Press",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats",
number = "6",
volume = "89",
doi = "10.1139/y11-043",
pages = "401-411"
}

Matić, S., Stanić, S., Bogojević, D., Vidaković, M., Grdović, N., Arambašić Jovanović, J., Dinić, S., Uskoković, A., Poznanović, G., Solujić, S., Mladenović, M., Marković, J.,& Mihailović, M.. (2011). Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats. in Canadian Journal of Physiology and Pharmacology
NRC Research Press; Canadian Science Publishing; National Research Council of Canada., 89(6), 401-411.
https://doi.org/10.1139/y11-043

Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Arambašić Jovanović J, Dinić S, Uskoković A, Poznanović G, Solujić S, Mladenović M, Marković J, Mihailović M. Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats. in Canadian Journal of Physiology and Pharmacology. 2011;89(6):401-411.
doi:10.1139/y11-043 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Vidaković, Melita, Grdović, Nevena, Arambašić Jovanović, Jelena, Dinić, Svetlana, Uskoković, Aleksandra, Poznanović, Goran, Solujić, Slavica, Mladenović, Milan, Marković, Jelena, Mihailović, Mirjana, "Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats" in Canadian Journal of Physiology and Pharmacology, 89, no. 6 (2011):401-411,
https://doi.org/10.1139/y11-043 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Solujić, Slavica
PY  - 2011
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-79957795403&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3207
AB  - The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.
T2  - International Journal of Molecular Sciences
T1  - In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.
IS  - 5
VL  - 12
DO  - 10.3390/ijms12052822
SP  - 2822
EP  - 2841
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Vuković, Nenad and Sukdolak, Slobodan and Solujić, Slavica",
year = "2011",
abstract = "The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.",
journal = "International Journal of Molecular Sciences",
title = "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.",
number = "5",
volume = "12",
doi = "10.3390/ijms12052822",
pages = "2822-2841"
}

Mladenović, M., Mihailović, M., Bogojević, D., Matić, S., Nićiforović, N., Mihailović, V., Vuković, N., Sukdolak, S.,& Solujić, S.. (2011). In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences, 12(5), 2822-2841.
https://doi.org/10.3390/ijms12052822

Mladenović M, Mihailović M, Bogojević D, Matić S, Nićiforović N, Mihailović V, Vuković N, Sukdolak S, Solujić S. In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences. 2011;12(5):2822-2841.
doi:10.3390/ijms12052822 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Vuković, Nenad, Sukdolak, Slobodan, Solujić, Slavica, "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies." in International Journal of Molecular Sciences, 12, no. 5 (2011):2822-2841,
https://doi.org/10.3390/ijms12052822 . .