TY  - JOUR
AU  - Sinan, Kouadio Ibrahime
AU  - Zengin, Gokhan
AU  - Fiorentino, Antonio
AU  - Vuotto, Chiara
AU  - Mahomoodally, Mohamad Fawzi
AU  - Jugreet, Sharmeen
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Etienne, Ouattara Katinan
AU  - Pereira, Catarina Guerreiro
AU  - Custódio, Luísa
AU  - Rengasamy, Kannan R.R.
AU  - D'Abrosca, Brigida
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S221242922200219X
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4985
AB  - Piliostigma thonningii (Fabaceae) is a versatile medicinal plant used as a traditional remedy, especially in African countries. In the present study, ethyl acetate, methanolic and water extracts of different parts of (fruits, leaves, stem barks) P. thonningii were evaluated for their bioactive contents, enzyme inhibitory, antioxidant and antimicrobial activities. Antioxidant potentials were also displayed in various methods like phosphomolybdenum assay (0.79–5.94 mmol TE/g), radical scavenging assays (DPPH: 20.39–471.28 mg TE/g; ABTS: 42.43–654.14 mg TE/g); reducing assays (CUPRAC: 102.98–1207.42 mg TE/g; FRAP: 45.30–656.25 mg TE/g) and metal chelating activity (2.55–22.32 mg EDTAE/g). In particular, the methanolic and water extracts showed better antioxidant potential. Except for a few, the extracts were also found to act as inhibitors of enzymes such as acetylcholinesterase (2.35–5.35 mg GALAE/g), butyrylcholinesterase (0.83–13.80 mg GALAE/g), tyrosinase (4.03–143.86 mg KAE/g), amylase (0.21–1.39 mmol ACAE/g) and glucosidase (3.52–7.13 mmol ACAE/g). Additionally, all the tested extracts possessed significant antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration: 0.11–0.61 mg/ml) and antifungal (MIC and minimum fungicidal concentration: 0.04–0.61 mg/ml) effects. Indeed, the data garnered from the present study provides a bright scope for the advanced investigation on this particular plant possessing pharmacologically active traits.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii
DO  - 10.1016/j.fbio.2022.101760
SP  - 101760
ER  - 

@article{
author = "Sinan, Kouadio Ibrahime and Zengin, Gokhan and Fiorentino, Antonio and Vuotto, Chiara and Mahomoodally, Mohamad Fawzi and Jugreet, Sharmeen and Glamočlija, Jasmina and Soković, Marina and Etienne, Ouattara Katinan and Pereira, Catarina Guerreiro and Custódio, Luísa and Rengasamy, Kannan R.R. and D'Abrosca, Brigida",
year = "2022",
abstract = "Piliostigma thonningii (Fabaceae) is a versatile medicinal plant used as a traditional remedy, especially in African countries. In the present study, ethyl acetate, methanolic and water extracts of different parts of (fruits, leaves, stem barks) P. thonningii were evaluated for their bioactive contents, enzyme inhibitory, antioxidant and antimicrobial activities. Antioxidant potentials were also displayed in various methods like phosphomolybdenum assay (0.79–5.94 mmol TE/g), radical scavenging assays (DPPH: 20.39–471.28 mg TE/g; ABTS: 42.43–654.14 mg TE/g); reducing assays (CUPRAC: 102.98–1207.42 mg TE/g; FRAP: 45.30–656.25 mg TE/g) and metal chelating activity (2.55–22.32 mg EDTAE/g). In particular, the methanolic and water extracts showed better antioxidant potential. Except for a few, the extracts were also found to act as inhibitors of enzymes such as acetylcholinesterase (2.35–5.35 mg GALAE/g), butyrylcholinesterase (0.83–13.80 mg GALAE/g), tyrosinase (4.03–143.86 mg KAE/g), amylase (0.21–1.39 mmol ACAE/g) and glucosidase (3.52–7.13 mmol ACAE/g). Additionally, all the tested extracts possessed significant antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration: 0.11–0.61 mg/ml) and antifungal (MIC and minimum fungicidal concentration: 0.04–0.61 mg/ml) effects. Indeed, the data garnered from the present study provides a bright scope for the advanced investigation on this particular plant possessing pharmacologically active traits.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii",
doi = "10.1016/j.fbio.2022.101760",
pages = "101760"
}

Sinan, K. I., Zengin, G., Fiorentino, A., Vuotto, C., Mahomoodally, M. F., Jugreet, S., Glamočlija, J., Soković, M., Etienne, O. K., Pereira, C. G., Custódio, L., Rengasamy, K. R.R.,& D'Abrosca, B.. (2022). Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii. in Food Bioscience
Elsevier Ltd., 101760.
https://doi.org/10.1016/j.fbio.2022.101760

Sinan KI, Zengin G, Fiorentino A, Vuotto C, Mahomoodally MF, Jugreet S, Glamočlija J, Soković M, Etienne OK, Pereira CG, Custódio L, Rengasamy KR, D'Abrosca B. Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii. in Food Bioscience. 2022;:101760.
doi:10.1016/j.fbio.2022.101760 .

Sinan, Kouadio Ibrahime, Zengin, Gokhan, Fiorentino, Antonio, Vuotto, Chiara, Mahomoodally, Mohamad Fawzi, Jugreet, Sharmeen, Glamočlija, Jasmina, Soković, Marina, Etienne, Ouattara Katinan, Pereira, Catarina Guerreiro, Custódio, Luísa, Rengasamy, Kannan R.R., D'Abrosca, Brigida, "Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii" in Food Bioscience (2022):101760,
https://doi.org/10.1016/j.fbio.2022.101760 . .

TY  - JOUR
AU  - Senizza, Biancamaria
AU  - Rocchetti, Gabriele
AU  - Sinan, Kouadio Ibrahime
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Etienne, Ouattara Katinan
AU  - Lucini, Luigi
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4171
AB  - Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties
VL  - 41
DO  - 10.1016/j.fbio.2021.100974
SP  - 100974
ER  - 

@article{
author = "Senizza, Biancamaria and Rocchetti, Gabriele and Sinan, Kouadio Ibrahime and Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Etienne, Ouattara Katinan and Lucini, Luigi",
year = "2021",
abstract = "Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties",
volume = "41",
doi = "10.1016/j.fbio.2021.100974",
pages = "100974"
}

Senizza, B., Rocchetti, G., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Glamočlija, J., Soković, M., Lobine, D., Etienne, O. K.,& Lucini, L.. (2021). The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience
Elsevier Ltd., 41, 100974.
https://doi.org/10.1016/j.fbio.2021.100974

Senizza B, Rocchetti G, Sinan KI, Zengin G, Mahomoodally MF, Glamočlija J, Soković M, Lobine D, Etienne OK, Lucini L. The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience. 2021;41:100974.
doi:10.1016/j.fbio.2021.100974 .

Senizza, Biancamaria, Rocchetti, Gabriele, Sinan, Kouadio Ibrahime, Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Etienne, Ouattara Katinan, Lucini, Luigi, "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties" in Food Bioscience, 41 (2021):100974,
https://doi.org/10.1016/j.fbio.2021.100974 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Stojković, Dejan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Jugreet, Bibi Sharmeen
AU  - Paksoy, Mehmet Yavuz
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Gallo, Monica
AU  - Montesano, Domenico
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/10/1510
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4487
AB  - Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl-and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Comprehensive biological and chemical evaluation of two seseli species (S. gummiferum and s. transcaucasicum)
IS  - 10
VL  - 10
DO  - 10.3390/antiox10101510
SP  - 1510
ER  - 

@article{
author = "Zengin, Gokhan and Stojković, Dejan and Mahomoodally, Mohamad Fawzi and Jugreet, Bibi Sharmeen and Paksoy, Mehmet Yavuz and Ivanov, Marija and Gašić, Uroš and Gallo, Monica and Montesano, Domenico",
year = "2021",
abstract = "Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl-and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Comprehensive biological and chemical evaluation of two seseli species (S. gummiferum and s. transcaucasicum)",
number = "10",
volume = "10",
doi = "10.3390/antiox10101510",
pages = "1510"
}

Zengin, G., Stojković, D., Mahomoodally, M. F., Jugreet, B. S., Paksoy, M. Y., Ivanov, M., Gašić, U., Gallo, M.,& Montesano, D.. (2021). Comprehensive biological and chemical evaluation of two seseli species (S. gummiferum and s. transcaucasicum). in Antioxidants
Basel: MDPI., 10(10), 1510.
https://doi.org/10.3390/antiox10101510

Zengin G, Stojković D, Mahomoodally MF, Jugreet BS, Paksoy MY, Ivanov M, Gašić U, Gallo M, Montesano D. Comprehensive biological and chemical evaluation of two seseli species (S. gummiferum and s. transcaucasicum). in Antioxidants. 2021;10(10):1510.
doi:10.3390/antiox10101510 .

Zengin, Gokhan, Stojković, Dejan, Mahomoodally, Mohamad Fawzi, Jugreet, Bibi Sharmeen, Paksoy, Mehmet Yavuz, Ivanov, Marija, Gašić, Uroš, Gallo, Monica, Montesano, Domenico, "Comprehensive biological and chemical evaluation of two seseli species (S. gummiferum and s. transcaucasicum)" in Antioxidants, 10, no. 10 (2021):1510,
https://doi.org/10.3390/antiox10101510 . .

TY  - JOUR
AU  - Rocchetti, Gabriele
AU  - Zengin, Gokhan
AU  - Cakmak, Yavuz Selim
AU  - Mahomoodally, Mohamad Fawzi
AU  - Kaya, Muhammed Fettah
AU  - Alsheikh, Sarah Mohammed
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Lucini, Luigi
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/123456789/3907
AB  - The genus Consolida is one of the most important within the Ranunculaceae family and several members of this genus contain important biologically active compounds, such as phenolics and alkaloids. The present study attempts for the first time to assess the biological properties and phytochemical constituents of six Consolida species (C. glandulosa (Boiss. & A. Huet) Bornm, C. hellospontica (Boiss.) Chater, C. raveyi (Boiss.) Schrödinger, C. regalis (Boiss.) Schrödinger, C. staminosa P.H. Davis & Sorger and C. stenocarpa (P.H. Davis & M. Hossain) P.H. Davis) growing in Turkey. A comprehensive phytochemical profiling of the different species was achieved by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry, targeting polyphenols and diterpene alkaloids. Also, the in vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), metal chelating and phosphomolybdenum) and enzyme inhibition (cholinesterases, tyrosinase, α-amylase, and α-glucosidase) potentials of the different Consolida methanolic extracts were evaluated. The UHPLC-QTOF profiling revealed 492 compounds, being 236 flavonoids, 93 phenolic acids, 78 tyrosol derivatives, 49 diterpene alkaloids, 29 lignans, and 7 stilbenes. The cumulative phenolic content ranged from 17.65 mg Eq./g for C. staminosa up to 43.04 mg Eq./g for C. glandulosa, whilst the total diterpene alkaloids content was exclusively found in C. glandulosa extracts (3.53 mg Eq./g). Consolida regalis (58. 61 mg trolox equivalent (TE)/g for DPPH and 65.38 mg TE/g for ABTS) and C. stenocarpa (46.81 mg TE/g for DPPH and 78.12 mg TE/g for ABTS) exhibited the highest anti-radical abilities, while the best reducing power values were recorded for C. glandulosa (129.46 mg TE/g for CUPRAC and 77.01 mg TE/g for FRAP) and C. raveyi (124.56 mg TE/g for CUPRAC and 78.36 mg TE/g for FRAP). Also, following the cholinesterases inhibition assays, C. hellospontica and C. glandulosa exhibited the highest inhibitory effects. Regarding tyrosinase inhibitory effects, C. raveyi showed the highest value, being 126.60 mg kojic acid equivalent (KAE)/g, followed by C. glandulosa (125.82 mg KAE/g) and C. stenocarpa (123.09 mg KAE/g). Besides, Consolida staminosa showed potential inhibition against α-amylase, but low glucosidase inhibitory properties. Therefore, the present findings could provide a starting point for further investigations to promote the industrial exploitation of these species with the aim of designing novel phyto-pharmaceuticals.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey
VL  - 158
DO  - 10.1016/j.indcrop.2020.112966
SP  - 112966
ER  - 

@article{
author = "Rocchetti, Gabriele and Zengin, Gokhan and Cakmak, Yavuz Selim and Mahomoodally, Mohamad Fawzi and Kaya, Muhammed Fettah and Alsheikh, Sarah Mohammed and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Lucini, Luigi",
year = "2020",
abstract = "The genus Consolida is one of the most important within the Ranunculaceae family and several members of this genus contain important biologically active compounds, such as phenolics and alkaloids. The present study attempts for the first time to assess the biological properties and phytochemical constituents of six Consolida species (C. glandulosa (Boiss. & A. Huet) Bornm, C. hellospontica (Boiss.) Chater, C. raveyi (Boiss.) Schrödinger, C. regalis (Boiss.) Schrödinger, C. staminosa P.H. Davis & Sorger and C. stenocarpa (P.H. Davis & M. Hossain) P.H. Davis) growing in Turkey. A comprehensive phytochemical profiling of the different species was achieved by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry, targeting polyphenols and diterpene alkaloids. Also, the in vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), metal chelating and phosphomolybdenum) and enzyme inhibition (cholinesterases, tyrosinase, α-amylase, and α-glucosidase) potentials of the different Consolida methanolic extracts were evaluated. The UHPLC-QTOF profiling revealed 492 compounds, being 236 flavonoids, 93 phenolic acids, 78 tyrosol derivatives, 49 diterpene alkaloids, 29 lignans, and 7 stilbenes. The cumulative phenolic content ranged from 17.65 mg Eq./g for C. staminosa up to 43.04 mg Eq./g for C. glandulosa, whilst the total diterpene alkaloids content was exclusively found in C. glandulosa extracts (3.53 mg Eq./g). Consolida regalis (58. 61 mg trolox equivalent (TE)/g for DPPH and 65.38 mg TE/g for ABTS) and C. stenocarpa (46.81 mg TE/g for DPPH and 78.12 mg TE/g for ABTS) exhibited the highest anti-radical abilities, while the best reducing power values were recorded for C. glandulosa (129.46 mg TE/g for CUPRAC and 77.01 mg TE/g for FRAP) and C. raveyi (124.56 mg TE/g for CUPRAC and 78.36 mg TE/g for FRAP). Also, following the cholinesterases inhibition assays, C. hellospontica and C. glandulosa exhibited the highest inhibitory effects. Regarding tyrosinase inhibitory effects, C. raveyi showed the highest value, being 126.60 mg kojic acid equivalent (KAE)/g, followed by C. glandulosa (125.82 mg KAE/g) and C. stenocarpa (123.09 mg KAE/g). Besides, Consolida staminosa showed potential inhibition against α-amylase, but low glucosidase inhibitory properties. Therefore, the present findings could provide a starting point for further investigations to promote the industrial exploitation of these species with the aim of designing novel phyto-pharmaceuticals.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey",
volume = "158",
doi = "10.1016/j.indcrop.2020.112966",
pages = "112966"
}

Rocchetti, G., Zengin, G., Cakmak, Y. S., Mahomoodally, M. F., Kaya, M. F., Alsheikh, S. M., Glamočlija, J., Soković, M., Lobine, D.,& Lucini, L.. (2020). A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey. in Industrial Crops and Products
Elsevier B.V.., 158, 112966.
https://doi.org/10.1016/j.indcrop.2020.112966

Rocchetti G, Zengin G, Cakmak YS, Mahomoodally MF, Kaya MF, Alsheikh SM, Glamočlija J, Soković M, Lobine D, Lucini L. A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey. in Industrial Crops and Products. 2020;158:112966.
doi:10.1016/j.indcrop.2020.112966 .

Rocchetti, Gabriele, Zengin, Gokhan, Cakmak, Yavuz Selim, Mahomoodally, Mohamad Fawzi, Kaya, Muhammed Fettah, Alsheikh, Sarah Mohammed, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Lucini, Luigi, "A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey" in Industrial Crops and Products, 158 (2020):112966,
https://doi.org/10.1016/j.indcrop.2020.112966 . .

TY  - JOUR
AU  - Chiavaroli, Annalisa
AU  - Sinan, Koaudio Ibrahime
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Bibi Sadeer, Nabeelah
AU  - Etienne, Ouattara Katinan
AU  - Cziáky, Zoltán
AU  - Jekő, József
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Recinella, Lucia
AU  - Brunetti, Luigi
AU  - Leone, Sheila
AU  - Abdallah, Hassan H.
AU  - Angelini, Paola
AU  - Angeles Flores, Giancarlo
AU  - Venanzoni, Roberto
AU  - Menghini, Luigi
AU  - Orlando, Giustino
AU  - Ferrante, Claudio
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/6/533
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3719
AB  - Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.
PB  - MDPI AG
T2  - Antioxidants
T1  - Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents
IS  - 6
VL  - 9
DO  - 10.3390/antiox9060533
SP  - 533
ER  - 

@article{
author = "Chiavaroli, Annalisa and Sinan, Koaudio Ibrahime and Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Bibi Sadeer, Nabeelah and Etienne, Ouattara Katinan and Cziáky, Zoltán and Jekő, József and Glamočlija, Jasmina and Soković, Marina and Recinella, Lucia and Brunetti, Luigi and Leone, Sheila and Abdallah, Hassan H. and Angelini, Paola and Angeles Flores, Giancarlo and Venanzoni, Roberto and Menghini, Luigi and Orlando, Giustino and Ferrante, Claudio",
year = "2020",
abstract = "Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents",
number = "6",
volume = "9",
doi = "10.3390/antiox9060533",
pages = "533"
}

Chiavaroli, A., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Bibi Sadeer, N., Etienne, O. K., Cziáky, Z., Jekő, J., Glamočlija, J., Soković, M., Recinella, L., Brunetti, L., Leone, S., Abdallah, H. H., Angelini, P., Angeles Flores, G., Venanzoni, R., Menghini, L., Orlando, G.,& Ferrante, C.. (2020). Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. in Antioxidants
MDPI AG., 9(6), 533.
https://doi.org/10.3390/antiox9060533

Chiavaroli A, Sinan KI, Zengin G, Mahomoodally MF, Bibi Sadeer N, Etienne OK, Cziáky Z, Jekő J, Glamočlija J, Soković M, Recinella L, Brunetti L, Leone S, Abdallah HH, Angelini P, Angeles Flores G, Venanzoni R, Menghini L, Orlando G, Ferrante C. Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. in Antioxidants. 2020;9(6):533.
doi:10.3390/antiox9060533 .

Chiavaroli, Annalisa, Sinan, Koaudio Ibrahime, Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Bibi Sadeer, Nabeelah, Etienne, Ouattara Katinan, Cziáky, Zoltán, Jekő, József, Glamočlija, Jasmina, Soković, Marina, Recinella, Lucia, Brunetti, Luigi, Leone, Sheila, Abdallah, Hassan H., Angelini, Paola, Angeles Flores, Giancarlo, Venanzoni, Roberto, Menghini, Luigi, Orlando, Giustino, Ferrante, Claudio, "Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents" in Antioxidants, 9, no. 6 (2020):533,
https://doi.org/10.3390/antiox9060533 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Sinan, Kouadio Ibrahime
AU  - Ak, Gunes
AU  - Mahomoodally, Mohamad Fawzi
AU  - Paksoy, Mehmet Yavuz
AU  - Picot-Allain, Carene
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Jekő, József
AU  - Cziáky, Zoltán
AU  - Rodrigues, Maria João
AU  - Pereira, Catarina G.
AU  - Custodio, Luisa
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3700
AB  - Several Apiaceae species, used as both food and in complementary and alternative medicine, represents a rich source of potential valuable phytopharmaceuticals which necessitates scientific contemplation. In the present study, the antioxidant, enzyme inhibitory, antimicrobial, and cytotoxic properties of methanol extracts of seven Apiaceae species, (Chaerophyllum macrospermum (Willd. ex Spreng.) Fisch. & C.A.Mey. ex Hohen, Ferula rigidula Fisch. ex DC., Ferula orientalis L., Prangos ferulacea Lindl., Prangos peucedanifolia Fenzl., Ferulago setifolia K. Koch, and Pimpinella anthriscoides Boiss.) were evaluated. Species belonging to the Prangos genus exhibited the highest total phenolic content, namely P. peucedanifolia and P. ferulacea, with values of 47.90 and 44.44 mg gallic acid equivalent/g extract, respectively. P. peucedanifolia also displayed the highest radical scavenging capacity (81.53 and 102.70 mg Trolox equivalent [TE]/g extract for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and reducing power (165.87 and 100.09 mg TE/g extract for cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). C. macrospermum showed the most potent inhibition against Alzheimer's disease related enzymes, namely acetylcholinesterase (4.53 mg galantamine equivalent [GALAE]/g extract) and butyrylcholinesterase (3.22 mg GALAE/g extract). P. ferulacea (131.94 mg kojic acid (KAE) equivalent/g extract) and P. peucedanifolia (4.97 mmol acarbose equivalent (ACAE)/g extract) were potent inhibitors of tyrosinase and α-glucosidase, respectively. In general, studied species were able to reduce cellular viabilities. P. peucedanifolia possessed promising antibacterial potential against Bacillus cereus (Minimum inhibition concentration (MIC): 0.37 mg/mL), L. monocytogenes (MIC: 0.56 mg/mL), P. aeruginosa and Escherichia coli (MIC: 0.27 mg/mL), Salmonella typhimurium and Enterobacter cloacae (MIC: 0.75 mg/mL). F. rigidula showed the highest antifungal effect against Aspergillus ochraceus and Trichoderma viride (MIC: 0.10 mg/mL). The present findings could be the scientific starting point towards the pharmaceutical and/or commercial utilization of these Apiaceae species.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study
VL  - 153
DO  - 10.1016/j.indcrop.2020.112572
SP  - 112572
ER  - 

@article{
author = "Zengin, Gokhan and Sinan, Kouadio Ibrahime and Ak, Gunes and Mahomoodally, Mohamad Fawzi and Paksoy, Mehmet Yavuz and Picot-Allain, Carene and Glamočlija, Jasmina and Soković, Marina and Jekő, József and Cziáky, Zoltán and Rodrigues, Maria João and Pereira, Catarina G. and Custodio, Luisa",
year = "2020",
abstract = "Several Apiaceae species, used as both food and in complementary and alternative medicine, represents a rich source of potential valuable phytopharmaceuticals which necessitates scientific contemplation. In the present study, the antioxidant, enzyme inhibitory, antimicrobial, and cytotoxic properties of methanol extracts of seven Apiaceae species, (Chaerophyllum macrospermum (Willd. ex Spreng.) Fisch. & C.A.Mey. ex Hohen, Ferula rigidula Fisch. ex DC., Ferula orientalis L., Prangos ferulacea Lindl., Prangos peucedanifolia Fenzl., Ferulago setifolia K. Koch, and Pimpinella anthriscoides Boiss.) were evaluated. Species belonging to the Prangos genus exhibited the highest total phenolic content, namely P. peucedanifolia and P. ferulacea, with values of 47.90 and 44.44 mg gallic acid equivalent/g extract, respectively. P. peucedanifolia also displayed the highest radical scavenging capacity (81.53 and 102.70 mg Trolox equivalent [TE]/g extract for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and reducing power (165.87 and 100.09 mg TE/g extract for cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). C. macrospermum showed the most potent inhibition against Alzheimer's disease related enzymes, namely acetylcholinesterase (4.53 mg galantamine equivalent [GALAE]/g extract) and butyrylcholinesterase (3.22 mg GALAE/g extract). P. ferulacea (131.94 mg kojic acid (KAE) equivalent/g extract) and P. peucedanifolia (4.97 mmol acarbose equivalent (ACAE)/g extract) were potent inhibitors of tyrosinase and α-glucosidase, respectively. In general, studied species were able to reduce cellular viabilities. P. peucedanifolia possessed promising antibacterial potential against Bacillus cereus (Minimum inhibition concentration (MIC): 0.37 mg/mL), L. monocytogenes (MIC: 0.56 mg/mL), P. aeruginosa and Escherichia coli (MIC: 0.27 mg/mL), Salmonella typhimurium and Enterobacter cloacae (MIC: 0.75 mg/mL). F. rigidula showed the highest antifungal effect against Aspergillus ochraceus and Trichoderma viride (MIC: 0.10 mg/mL). The present findings could be the scientific starting point towards the pharmaceutical and/or commercial utilization of these Apiaceae species.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study",
volume = "153",
doi = "10.1016/j.indcrop.2020.112572",
pages = "112572"
}

Zengin, G., Sinan, K. I., Ak, G., Mahomoodally, M. F., Paksoy, M. Y., Picot-Allain, C., Glamočlija, J., Soković, M., Jekő, J., Cziáky, Z., Rodrigues, M. J., Pereira, C. G.,& Custodio, L.. (2020). Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study. in Industrial Crops and Products
Elsevier B.V.., 153, 112572.
https://doi.org/10.1016/j.indcrop.2020.112572

Zengin G, Sinan KI, Ak G, Mahomoodally MF, Paksoy MY, Picot-Allain C, Glamočlija J, Soković M, Jekő J, Cziáky Z, Rodrigues MJ, Pereira CG, Custodio L. Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study. in Industrial Crops and Products. 2020;153:112572.
doi:10.1016/j.indcrop.2020.112572 .

Zengin, Gokhan, Sinan, Kouadio Ibrahime, Ak, Gunes, Mahomoodally, Mohamad Fawzi, Paksoy, Mehmet Yavuz, Picot-Allain, Carene, Glamočlija, Jasmina, Soković, Marina, Jekő, József, Cziáky, Zoltán, Rodrigues, Maria João, Pereira, Catarina G., Custodio, Luisa, "Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study" in Industrial Crops and Products, 153 (2020):112572,
https://doi.org/10.1016/j.indcrop.2020.112572 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Cvetanović, Aleksandra
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Stupar, Alena
AU  - Uysal, Ahmet
AU  - Şenkardes, Ismail
AU  - Sinan, Kouadio Ibrahime
AU  - Picot-Allain, Marie Carene Nancy
AU  - Ak, Gunes
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3769
AB  - The growing awareness on implications related to the use of harsh chemicals and non-sustainable extraction
methods of bioactive compounds from plants is causing a paradigm shift towards a more eco-friendly approach.
The present study sets out to investigate the possible variation in the antioxidant, mutagenic/antimutagenic,
antimicrobial, and enzyme inhibitory properties of Origanum vulgare subsp. viridulum extracts obtained using
conventional, namely, maceration and Soxhlet extraction, and non-conventional, namely, ultrasound-assisted
(UAE), microwave-assisted (MAE), and accelerated solvent extraction (ASE), techniques. Also, chemical char-
acterization of these extracts were performed via ultrahigh-performance liquid chromatography and mass
spectrometry (UHPLC-LTQ OrbiTrap MS) technique. A total of 58 polyphenols were identified in the tested
extracts. Highest phenolic content was obtained from ASE (186.63 mg gallic acid equivalent [GAE]/g) and MAE
(170.90 mg GAE/g) extracts. Maceration extract (18.89 mg rutin equivalent [RE]/g) showed highest flavonoid
content. ASE extract showed highest activity in the DPPH (359.45 mg Trolox equivalent [TE]/g), ABTS
(679.63 mg TE/g), FRAP (1433.94 mg TE/g), and CUPRAC (1685.72 mg TE/g) assays. In terms of enzyme in-
hibition, the UAE extract showed highest activity against acetyl cholinesterase (4.55 mg galantamine equivalent
[GALAE]/g), butyryl cholinesterase (6.12 mg GALAE/g), and tyrosinase (173.87 mg kojic acid equivalent
[KAE]/g) compared with the other extracts. The extracts exhibited significant antimutagenic and antimicrobial
properties. Data collected from this study support the use of ASE and UAE in the extraction of bioactive com-
pounds from Origanum vulgare subsp. viridulum aerial parts.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods
VL  - 154
DO  - 10.1016/j.indcrop.2020.112747
SP  - 112747
ER  - 

@article{
author = "Zengin, Gokhan and Cvetanović, Aleksandra and Gašić, Uroš and Dragićević, Milan and Stupar, Alena and Uysal, Ahmet and Şenkardes, Ismail and Sinan, Kouadio Ibrahime and Picot-Allain, Marie Carene Nancy and Ak, Gunes and Mahomoodally, Mohamad Fawzi",
year = "2020",
abstract = "The growing awareness on implications related to the use of harsh chemicals and non-sustainable extraction
methods of bioactive compounds from plants is causing a paradigm shift towards a more eco-friendly approach.
The present study sets out to investigate the possible variation in the antioxidant, mutagenic/antimutagenic,
antimicrobial, and enzyme inhibitory properties of Origanum vulgare subsp. viridulum extracts obtained using
conventional, namely, maceration and Soxhlet extraction, and non-conventional, namely, ultrasound-assisted
(UAE), microwave-assisted (MAE), and accelerated solvent extraction (ASE), techniques. Also, chemical char-
acterization of these extracts were performed via ultrahigh-performance liquid chromatography and mass
spectrometry (UHPLC-LTQ OrbiTrap MS) technique. A total of 58 polyphenols were identified in the tested
extracts. Highest phenolic content was obtained from ASE (186.63 mg gallic acid equivalent [GAE]/g) and MAE
(170.90 mg GAE/g) extracts. Maceration extract (18.89 mg rutin equivalent [RE]/g) showed highest flavonoid
content. ASE extract showed highest activity in the DPPH (359.45 mg Trolox equivalent [TE]/g), ABTS
(679.63 mg TE/g), FRAP (1433.94 mg TE/g), and CUPRAC (1685.72 mg TE/g) assays. In terms of enzyme in-
hibition, the UAE extract showed highest activity against acetyl cholinesterase (4.55 mg galantamine equivalent
[GALAE]/g), butyryl cholinesterase (6.12 mg GALAE/g), and tyrosinase (173.87 mg kojic acid equivalent
[KAE]/g) compared with the other extracts. The extracts exhibited significant antimutagenic and antimicrobial
properties. Data collected from this study support the use of ASE and UAE in the extraction of bioactive com-
pounds from Origanum vulgare subsp. viridulum aerial parts.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods",
volume = "154",
doi = "10.1016/j.indcrop.2020.112747",
pages = "112747"
}

Zengin, G., Cvetanović, A., Gašić, U., Dragićević, M., Stupar, A., Uysal, A., Şenkardes, I., Sinan, K. I., Picot-Allain, M. C. N., Ak, G.,& Mahomoodally, M. F.. (2020). UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods. in Industrial Crops & Products
Elsevier., 154, 112747.
https://doi.org/10.1016/j.indcrop.2020.112747

Zengin G, Cvetanović A, Gašić U, Dragićević M, Stupar A, Uysal A, Şenkardes I, Sinan KI, Picot-Allain MCN, Ak G, Mahomoodally MF. UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods. in Industrial Crops & Products. 2020;154:112747.
doi:10.1016/j.indcrop.2020.112747 .

Zengin, Gokhan, Cvetanović, Aleksandra, Gašić, Uroš, Dragićević, Milan, Stupar, Alena, Uysal, Ahmet, Şenkardes, Ismail, Sinan, Kouadio Ibrahime, Picot-Allain, Marie Carene Nancy, Ak, Gunes, Mahomoodally, Mohamad Fawzi, "UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods" in Industrial Crops & Products, 154 (2020):112747,
https://doi.org/10.1016/j.indcrop.2020.112747 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Cvetanović, Aleksandra
AU  - Gašić, Uroš
AU  - Stupar, Alena
AU  - Bulut, Gizem
AU  - Senkardes, Ismail
AU  - Dogan, Ahmet
AU  - Sinan, Kouadio Ibrahime
AU  - Uysal, Sengul
AU  - Aumeeruddy-Elalfi, Zaahiri
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2020
UR  - https://doi.org/10.1016/j.indcrop.2020.112202
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3609
AB  - Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.
T2  - Industrial Crops & Products
T1  - Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip
IS  - April 2020
VL  - 146
DO  - 10.1016/j.indcrop.2020.112202
SP  - 112202
ER  - 

@article{
author = "Zengin, Gokhan and Cvetanović, Aleksandra and Gašić, Uroš and Stupar, Alena and Bulut, Gizem and Senkardes, Ismail and Dogan, Ahmet and Sinan, Kouadio Ibrahime and Uysal, Sengul and Aumeeruddy-Elalfi, Zaahiri and Aktumsek, Abdurrahman and Mahomoodally, Mohamad Fawzi",
year = "2020",
abstract = "Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.",
journal = "Industrial Crops & Products",
title = "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip",
number = "April 2020",
volume = "146",
doi = "10.1016/j.indcrop.2020.112202",
pages = "112202"
}

Zengin, G., Cvetanović, A., Gašić, U., Stupar, A., Bulut, G., Senkardes, I., Dogan, A., Sinan, K. I., Uysal, S., Aumeeruddy-Elalfi, Z., Aktumsek, A.,& Mahomoodally, M. F.. (2020). Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products, 146(April 2020), 112202.
https://doi.org/10.1016/j.indcrop.2020.112202

Zengin G, Cvetanović A, Gašić U, Stupar A, Bulut G, Senkardes I, Dogan A, Sinan KI, Uysal S, Aumeeruddy-Elalfi Z, Aktumsek A, Mahomoodally MF. Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products. 2020;146(April 2020):112202.
doi:10.1016/j.indcrop.2020.112202 .

Zengin, Gokhan, Cvetanović, Aleksandra, Gašić, Uroš, Stupar, Alena, Bulut, Gizem, Senkardes, Ismail, Dogan, Ahmet, Sinan, Kouadio Ibrahime, Uysal, Sengul, Aumeeruddy-Elalfi, Zaahiri, Aktumsek, Abdurrahman, Mahomoodally, Mohamad Fawzi, "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip" in Industrial Crops & Products, 146, no. April 2020 (2020):112202,
https://doi.org/10.1016/j.indcrop.2020.112202 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Paksoy, Mehmet Yavuz
AU  - Aumeeruddy, Muhammad Zakariyyah
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Diuzheva, Alina
AU  - Jekő, József
AU  - Cziáky, Zoltán
AU  - Rodrigues, Maria João
AU  - Custodio, Luisa
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0963996919303163?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3407
AB  - Bunium species have been reported to be used both as food and in traditional medicines. The scientific community has attempted to probe into the pharmacological and chemical profiles of this genus. Nonetheless, many species have not been investigated fully to date. In this study, we determined the phenolic components, antimicrobial, antioxidant, and enzyme inhibitory activities of aerial parts of four Bunium species (B. sayai, B. pinnatifolium, B. brachyactis and B. macrocarpum). Results showed that B. microcarpum and B. pinnatifolium were strong antioxidants as evidenced in the DPPH, ABTS, CUPRAC, and FRAP assays. B. brachyactis was the most effective metal chelator, and displayed high enzyme inhibition against cholinesterase, tyrosinase, amylase, glucosidase, and lipase. The four species showed varied antimicrobial activity against each microorganism. Overall, they showed high activity against P. mirabilis and E. coli (MIC and MBC <1 mg mL−1). B. brachyactis was more effective against Aspergillus versicolor compared to the standard drug ketoconazole. B. brachyactis was also more effective than both ketoconazole and bifonazole against Trichoderma viride. B. sayai was more effective than ketoconazole in inhibiting A. fumigatus. B. sayai was most non-toxic to HEK 293 (cellular viability = 117%) and HepG2 (cellular viability = 104%). The highest level of TPC was observed in B. pinnatifolium (35.94 mg GAE g−1) while B. microcarpum possessed the highest TFC (39.21 mg RE g−1). Seventy four compounds were detected in B. microcarpum, 70 in B. brachyactis, 66 in B. sayai, and 51 in B. pinnatifolium. Quinic acid, chlorogenic acid, pantothenic acid, esculin, isoquercitrin, rutin, apigenin, and scopoletin were present in all the four species. This study showed that the four Bunium species are good sources of biologically active compounds with pharmaceutical and nutraceutical potential.
T2  - Food Research International
T1  - New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species
VL  - 123
DO  - 10.1016/J.FOODRES.2019.05.013
SP  - 414
EP  - 424
ER  - 

@article{
author = "Zengin, Gokhan and Paksoy, Mehmet Yavuz and Aumeeruddy, Muhammad Zakariyyah and Glamočlija, Jasmina and Soković, Marina and Diuzheva, Alina and Jekő, József and Cziáky, Zoltán and Rodrigues, Maria João and Custodio, Luisa and Mahomoodally, Mohamad Fawzi",
year = "2019",
abstract = "Bunium species have been reported to be used both as food and in traditional medicines. The scientific community has attempted to probe into the pharmacological and chemical profiles of this genus. Nonetheless, many species have not been investigated fully to date. In this study, we determined the phenolic components, antimicrobial, antioxidant, and enzyme inhibitory activities of aerial parts of four Bunium species (B. sayai, B. pinnatifolium, B. brachyactis and B. macrocarpum). Results showed that B. microcarpum and B. pinnatifolium were strong antioxidants as evidenced in the DPPH, ABTS, CUPRAC, and FRAP assays. B. brachyactis was the most effective metal chelator, and displayed high enzyme inhibition against cholinesterase, tyrosinase, amylase, glucosidase, and lipase. The four species showed varied antimicrobial activity against each microorganism. Overall, they showed high activity against P. mirabilis and E. coli (MIC and MBC <1 mg mL−1). B. brachyactis was more effective against Aspergillus versicolor compared to the standard drug ketoconazole. B. brachyactis was also more effective than both ketoconazole and bifonazole against Trichoderma viride. B. sayai was more effective than ketoconazole in inhibiting A. fumigatus. B. sayai was most non-toxic to HEK 293 (cellular viability = 117%) and HepG2 (cellular viability = 104%). The highest level of TPC was observed in B. pinnatifolium (35.94 mg GAE g−1) while B. microcarpum possessed the highest TFC (39.21 mg RE g−1). Seventy four compounds were detected in B. microcarpum, 70 in B. brachyactis, 66 in B. sayai, and 51 in B. pinnatifolium. Quinic acid, chlorogenic acid, pantothenic acid, esculin, isoquercitrin, rutin, apigenin, and scopoletin were present in all the four species. This study showed that the four Bunium species are good sources of biologically active compounds with pharmaceutical and nutraceutical potential.",
journal = "Food Research International",
title = "New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species",
volume = "123",
doi = "10.1016/J.FOODRES.2019.05.013",
pages = "414-424"
}

Zengin, G., Paksoy, M. Y., Aumeeruddy, M. Z., Glamočlija, J., Soković, M., Diuzheva, A., Jekő, J., Cziáky, Z., Rodrigues, M. J., Custodio, L.,& Mahomoodally, M. F.. (2019). New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species. in Food Research International, 123, 414-424.
https://doi.org/10.1016/J.FOODRES.2019.05.013

Zengin G, Paksoy MY, Aumeeruddy MZ, Glamočlija J, Soković M, Diuzheva A, Jekő J, Cziáky Z, Rodrigues MJ, Custodio L, Mahomoodally MF. New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species. in Food Research International. 2019;123:414-424.
doi:10.1016/J.FOODRES.2019.05.013 .

Zengin, Gokhan, Paksoy, Mehmet Yavuz, Aumeeruddy, Muhammad Zakariyyah, Glamočlija, Jasmina, Soković, Marina, Diuzheva, Alina, Jekő, József, Cziáky, Zoltán, Rodrigues, Maria João, Custodio, Luisa, Mahomoodally, Mohamad Fawzi, "New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species" in Food Research International, 123 (2019):414-424,
https://doi.org/10.1016/J.FOODRES.2019.05.013 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Paksoy, Mehmet Yavuz
AU  - Picot-Allain, Carene
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Diuzheva, Alina
AU  - Jekő, József
AU  - Cziáky, Zoltán
AU  - Rodrigues, Maria João
AU  - Sinan, Kouadio Ibrahime
AU  - Custodio, Luisa
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0926669019302833?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3337
AB  - Several species of the Apiaceae family have been employed in traditional cultures for their curative virtues. The present study focused on five Apiaceae species, (Falcaria vulgaris (FV), Smyrniopsis aucheri (SA), Smyrniopsis munzurdagensis (SM), Smyrnium cordifolium (SC), and Actinolema macrolema (AM)). The antioxidant, enzyme inhibitory (α-amylase, α-glucosidase, acetyl- and butyrylcholinesterase, lipase, and tyrosinase), antimicrobial, phytochemical, and cytotoxicity profiles of the methanol extracts of the selected Apiaceae species were determined. SC extract (35.68 mg gallic acid equivalent/g extract) possessed the highest phenolic content while the AM extract (56.79 mg rutin equivalent/g extract) had the highest flavonoid content. HPLC-ESI-MS (High performance liquid chromatography-electrospray tandem mass spectrometry) analyses showed presence of ferulic acid in all the five species. SC extract exhibited high radical scavenging (59.28 and 94.31 mg Trolox equivalent [TE]/g extract, DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid), respectively) and reducing activity (161.44 and 113.62 mg TE/g extract, for CUPRAC (cupric reducing antioxidant capacity) and FRAP (ferric reducing antioxidant power), respectively). SM extract exhibited the highest cholinesterase’s inhibitory action (3.82 and 4.76 mg galantamine equivalent/g extract, for acetyl- and butyrylcholinesterase, respectively). The extracts showed higher inhibition against α-glucosidase (7.32-11.99 mmol acarbose equivalent [ACAE]/g extract) compared to α-amylase (0.51-0.55 mmol ACAE/g extract). SC extract was the most active (137.54 mg kojic acid equivalent/g extract) tyrosinase inhibitor and FV extract (113.75 mg Orlistat equivalent/g) the best lipase inhibitor. SM extract showed potent antibacterial effect against B. cereus (MIC (minimum inhibitory concentration) 0.180 mg/mL), P. mirabilis (MIC 0.180 mg/mL), M. flavus (MIC 0.560 mg/mL), P. aeruginosa (MIC 0.275 mg/mL), and S. typhimurium (MIC 1.500 mg/mL). FV extract (MIC 0.140 mg/mL) suppressed A. fumigatus growth. Cytotoxicity was assessed on murine macrophage (RAW 264.7), human embryonic kidney (HEK 293), and human hepatocellular carcinoma (HepG2) cell lines. FV (60.3%) and SM (57.4%) showed the highest reduction on RAW 264.7 cellular viability, whereas SM (74.1%) showed toxicity against HepG2. This study supports that the Apiaceae species could be considered as promising candidates for the development of novel pharmacophores for the management of several human ailments.
T2  - Industrial Crops and Products
T1  - Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients
VL  - 135
DO  - 10.1016/J.INDCROP.2019.04.033
SP  - 107
EP  - 121
ER  - 

@article{
author = "Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Paksoy, Mehmet Yavuz and Picot-Allain, Carene and Glamočlija, Jasmina and Soković, Marina and Diuzheva, Alina and Jekő, József and Cziáky, Zoltán and Rodrigues, Maria João and Sinan, Kouadio Ibrahime and Custodio, Luisa",
year = "2019",
abstract = "Several species of the Apiaceae family have been employed in traditional cultures for their curative virtues. The present study focused on five Apiaceae species, (Falcaria vulgaris (FV), Smyrniopsis aucheri (SA), Smyrniopsis munzurdagensis (SM), Smyrnium cordifolium (SC), and Actinolema macrolema (AM)). The antioxidant, enzyme inhibitory (α-amylase, α-glucosidase, acetyl- and butyrylcholinesterase, lipase, and tyrosinase), antimicrobial, phytochemical, and cytotoxicity profiles of the methanol extracts of the selected Apiaceae species were determined. SC extract (35.68 mg gallic acid equivalent/g extract) possessed the highest phenolic content while the AM extract (56.79 mg rutin equivalent/g extract) had the highest flavonoid content. HPLC-ESI-MS (High performance liquid chromatography-electrospray tandem mass spectrometry) analyses showed presence of ferulic acid in all the five species. SC extract exhibited high radical scavenging (59.28 and 94.31 mg Trolox equivalent [TE]/g extract, DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid), respectively) and reducing activity (161.44 and 113.62 mg TE/g extract, for CUPRAC (cupric reducing antioxidant capacity) and FRAP (ferric reducing antioxidant power), respectively). SM extract exhibited the highest cholinesterase’s inhibitory action (3.82 and 4.76 mg galantamine equivalent/g extract, for acetyl- and butyrylcholinesterase, respectively). The extracts showed higher inhibition against α-glucosidase (7.32-11.99 mmol acarbose equivalent [ACAE]/g extract) compared to α-amylase (0.51-0.55 mmol ACAE/g extract). SC extract was the most active (137.54 mg kojic acid equivalent/g extract) tyrosinase inhibitor and FV extract (113.75 mg Orlistat equivalent/g) the best lipase inhibitor. SM extract showed potent antibacterial effect against B. cereus (MIC (minimum inhibitory concentration) 0.180 mg/mL), P. mirabilis (MIC 0.180 mg/mL), M. flavus (MIC 0.560 mg/mL), P. aeruginosa (MIC 0.275 mg/mL), and S. typhimurium (MIC 1.500 mg/mL). FV extract (MIC 0.140 mg/mL) suppressed A. fumigatus growth. Cytotoxicity was assessed on murine macrophage (RAW 264.7), human embryonic kidney (HEK 293), and human hepatocellular carcinoma (HepG2) cell lines. FV (60.3%) and SM (57.4%) showed the highest reduction on RAW 264.7 cellular viability, whereas SM (74.1%) showed toxicity against HepG2. This study supports that the Apiaceae species could be considered as promising candidates for the development of novel pharmacophores for the management of several human ailments.",
journal = "Industrial Crops and Products",
title = "Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients",
volume = "135",
doi = "10.1016/J.INDCROP.2019.04.033",
pages = "107-121"
}

Zengin, G., Mahomoodally, M. F., Paksoy, M. Y., Picot-Allain, C., Glamočlija, J., Soković, M., Diuzheva, A., Jekő, J., Cziáky, Z., Rodrigues, M. J., Sinan, K. I.,& Custodio, L.. (2019). Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients. in Industrial Crops and Products, 135, 107-121.
https://doi.org/10.1016/J.INDCROP.2019.04.033

Zengin G, Mahomoodally MF, Paksoy MY, Picot-Allain C, Glamočlija J, Soković M, Diuzheva A, Jekő J, Cziáky Z, Rodrigues MJ, Sinan KI, Custodio L. Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients. in Industrial Crops and Products. 2019;135:107-121.
doi:10.1016/J.INDCROP.2019.04.033 .

Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Paksoy, Mehmet Yavuz, Picot-Allain, Carene, Glamočlija, Jasmina, Soković, Marina, Diuzheva, Alina, Jekő, József, Cziáky, Zoltán, Rodrigues, Maria João, Sinan, Kouadio Ibrahime, Custodio, Luisa, "Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients" in Industrial Crops and Products, 135 (2019):107-121,
https://doi.org/10.1016/J.INDCROP.2019.04.033 . .