TY  - JOUR
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ristić, Mihailo
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Perišić, Mirjana
AU  - Puač, Nevena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4394
AB  - When exposed to stressful conditions, plants produce numerous volatile organic compounds (VOCs) that have different biological and environmental functions. VOCs emitted during the rehydration process by the fronds of desiccation tolerant fern Asplenium ceterach L. were investigated. Headspace GC–MS analysis revealed that the volatiles profile of rustyback fern is mainly composed of fatty acid derivatives: isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols, and related compounds. Aerial parts of the rustyback fern do not contain monoterpene-type, sesquiterpene-type, and diterpene-type hydrocarbons or corresponding terpenoids. Online detection of VOCs using proton-transfer reaction mass spectrometry (PTR–MS) showed a significant increase in emission intensity of dominant volatiles during the first hours of the rehydration process. Twelve hours after re-watering, emission of detected volatiles had returned to the basal levels that corresponded to hydrated plants. During the early phase of rehydration malondialdehyde (MDA) content in fronds, as an indicator of membrane damage, decreased rapidly which implies that lipoxygenase activity is not stimulated during the recovery process of rustyback fern.
PB  - Basel: MDPI
T2  - Biology
T1  - Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds
IS  - 7
VL  - 10
DO  - 10.3390/biology10070574
SP  - 574
ER  - 

@article{
author = "Živković, Suzana and Skorić, Marijana and Ristić, Mihailo and Filipović, Biljana and Milutinović, Milica and Perišić, Mirjana and Puač, Nevena",
year = "2021",
abstract = "When exposed to stressful conditions, plants produce numerous volatile organic compounds (VOCs) that have different biological and environmental functions. VOCs emitted during the rehydration process by the fronds of desiccation tolerant fern Asplenium ceterach L. were investigated. Headspace GC–MS analysis revealed that the volatiles profile of rustyback fern is mainly composed of fatty acid derivatives: isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols, and related compounds. Aerial parts of the rustyback fern do not contain monoterpene-type, sesquiterpene-type, and diterpene-type hydrocarbons or corresponding terpenoids. Online detection of VOCs using proton-transfer reaction mass spectrometry (PTR–MS) showed a significant increase in emission intensity of dominant volatiles during the first hours of the rehydration process. Twelve hours after re-watering, emission of detected volatiles had returned to the basal levels that corresponded to hydrated plants. During the early phase of rehydration malondialdehyde (MDA) content in fronds, as an indicator of membrane damage, decreased rapidly which implies that lipoxygenase activity is not stimulated during the recovery process of rustyback fern.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds",
number = "7",
volume = "10",
doi = "10.3390/biology10070574",
pages = "574"
}

Živković, S., Skorić, M., Ristić, M., Filipović, B., Milutinović, M., Perišić, M.,& Puač, N.. (2021). Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds. in Biology
Basel: MDPI., 10(7), 574.
https://doi.org/10.3390/biology10070574

Živković S, Skorić M, Ristić M, Filipović B, Milutinović M, Perišić M, Puač N. Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds. in Biology. 2021;10(7):574.
doi:10.3390/biology10070574 .

Živković, Suzana, Skorić, Marijana, Ristić, Mihailo, Filipović, Biljana, Milutinović, Milica, Perišić, Mirjana, Puač, Nevena, "Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds" in Biology, 10, no. 7 (2021):574,
https://doi.org/10.3390/biology10070574 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Anđelković, Boban
AU  - Aranđelović, Sandra
AU  - Radulović, Siniša
AU  - Soković, Marina
AU  - Krstić Milošević, Dijana
AU  - Ristić, Mihailo
AU  - Ćalić, Dušica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0254629916339928
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2721
AB  - Chemical composition of essential oil (EO) and methanol extracts (MEs) from different parts of Tanacetum vulgare L. plant was analyzed and investigated for potential biological activities and correlated with the main constituents detected in EO and MEs. The EO was characterized by a high content of oxygenated monoterpenes with trans-chrysanthenyl acetate as major compound. All MEs were characterized by neochlorogenic, 3,5-O-dicaffeoylquinic and caffeoylquinic acids. High phenolic content in MEs correlated to high antioxidant capacity, especially for roots. Tansy EO showed strong activity against the most of tested fungi and was efficient as bifonazole and ketoconazole, while the most sensitive bacteria were Gram-negative E. coli and E. cloacae. All MEs showed fungistatic and fungicidal effects against all of the eight tested fungi, but the antimicrobial activity was higher against Gram-positive bacteria. Additionally, shown for the first time, MEs of leaves and flowers exhibited a strong antiproliferative effect on human cervical adenocarcinoma (HeLa) cells, causing cell shrinkage and detachment. In the present study, the Tansy extracts and essential oil with low thujone content can provide a very promising and effective alternative in the field of antimicrobial applications and food preservation. The Tansy MEs possess a high antioxidant potential with phenolic acids being a major radical scavenging contributor with the proved antiproliferative activity to HeLa cells.
T2  - South African Journal of Botany
T1  - Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts
VL  - 111
DO  - 10.1016/j.sajb.2017.03.028
SP  - 212
EP  - 221
ER  - 

@article{
author = "Devrnja, Nina and Anđelković, Boban and Aranđelović, Sandra and Radulović, Siniša and Soković, Marina and Krstić Milošević, Dijana and Ristić, Mihailo and Ćalić, Dušica",
year = "2017",
abstract = "Chemical composition of essential oil (EO) and methanol extracts (MEs) from different parts of Tanacetum vulgare L. plant was analyzed and investigated for potential biological activities and correlated with the main constituents detected in EO and MEs. The EO was characterized by a high content of oxygenated monoterpenes with trans-chrysanthenyl acetate as major compound. All MEs were characterized by neochlorogenic, 3,5-O-dicaffeoylquinic and caffeoylquinic acids. High phenolic content in MEs correlated to high antioxidant capacity, especially for roots. Tansy EO showed strong activity against the most of tested fungi and was efficient as bifonazole and ketoconazole, while the most sensitive bacteria were Gram-negative E. coli and E. cloacae. All MEs showed fungistatic and fungicidal effects against all of the eight tested fungi, but the antimicrobial activity was higher against Gram-positive bacteria. Additionally, shown for the first time, MEs of leaves and flowers exhibited a strong antiproliferative effect on human cervical adenocarcinoma (HeLa) cells, causing cell shrinkage and detachment. In the present study, the Tansy extracts and essential oil with low thujone content can provide a very promising and effective alternative in the field of antimicrobial applications and food preservation. The Tansy MEs possess a high antioxidant potential with phenolic acids being a major radical scavenging contributor with the proved antiproliferative activity to HeLa cells.",
journal = "South African Journal of Botany",
title = "Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts",
volume = "111",
doi = "10.1016/j.sajb.2017.03.028",
pages = "212-221"
}

Devrnja, N., Anđelković, B., Aranđelović, S., Radulović, S., Soković, M., Krstić Milošević, D., Ristić, M.,& Ćalić, D.. (2017). Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts. in South African Journal of Botany, 111, 212-221.
https://doi.org/10.1016/j.sajb.2017.03.028

Devrnja N, Anđelković B, Aranđelović S, Radulović S, Soković M, Krstić Milošević D, Ristić M, Ćalić D. Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts. in South African Journal of Botany. 2017;111:212-221.
doi:10.1016/j.sajb.2017.03.028 .

Devrnja, Nina, Anđelković, Boban, Aranđelović, Sandra, Radulović, Siniša, Soković, Marina, Krstić Milošević, Dijana, Ristić, Mihailo, Ćalić, Dušica, "Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts" in South African Journal of Botany, 111 (2017):212-221,
https://doi.org/10.1016/j.sajb.2017.03.028 . .

TY  - JOUR
AU  - Mileski, Ksenija Slave
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Ristić, Mihailo S
AU  - Matevski, Vlado S
AU  - Marin, Petar D
AU  - Džamić, Ana M.
PY  - 2017
UR  - https://ojs.openagrar.de/index.php/JABFQ/article/view/6507
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3031
AB  - This study shows Laserpitium ochridanum essential oil composition, its antifungal potency, and possible antioxidant, antimicrobial and anti-quorum sensing activities of different extracts. Monoterpene hydrocarbons (40.9%) found to be the most abundant group of constituents in the oil. Sabinene (22.8%), viridiflorol (14.7%) and α-pinene (11.40%) were the main compounds of the oil. Ethanolic extract had the highest antioxidant capacity in DPPH and ABTS assays and was the richest in polyphenols. Microdilution method revealed the strongest antibacterial activity of ethanolic extracts in comparison to other tested extracts and streptomycin. L. ochridanum oil evidenced the best antifungal potential against used micromycetes. Results of anti-quorum sensing activity assay indicated high affection of aqueous extract in reduction of PAO1 pyocianin production (18.07%).Used samples possessed slight reduction of twitching and swimming mobility. Excellent antifungal oil activity, good antioxidant, antimicrobial and anti-quorum activity of L. ochridanum extracts was found. This study shows for the first time anti-guorum sensing activity against Pseudomonas aeruginosa of L. ochridanum, as well as its significant antioxidant potential. This study was conducted due to adventives of usage of natural products as efficient substitution for synthetic agents in treatment of various medical conditions.
T2  - Journal of Applied Botany and Food Quality
T1  - Laserpitium ochridanum: antioxidant, antimicrobial and anti-quorum sensing activities against Pseudomonas aeruginosa
VL  - 90
DO  - 10.5073/jabfq.2017.090.041
SP  - 330
EP  - 338
ER  - 

@article{
author = "Mileski, Ksenija Slave and Ćirić, Ana and Petrović, Jovana and Ristić, Mihailo S and Matevski, Vlado S and Marin, Petar D and Džamić, Ana M.",
year = "2017",
abstract = "This study shows Laserpitium ochridanum essential oil composition, its antifungal potency, and possible antioxidant, antimicrobial and anti-quorum sensing activities of different extracts. Monoterpene hydrocarbons (40.9%) found to be the most abundant group of constituents in the oil. Sabinene (22.8%), viridiflorol (14.7%) and α-pinene (11.40%) were the main compounds of the oil. Ethanolic extract had the highest antioxidant capacity in DPPH and ABTS assays and was the richest in polyphenols. Microdilution method revealed the strongest antibacterial activity of ethanolic extracts in comparison to other tested extracts and streptomycin. L. ochridanum oil evidenced the best antifungal potential against used micromycetes. Results of anti-quorum sensing activity assay indicated high affection of aqueous extract in reduction of PAO1 pyocianin production (18.07%).Used samples possessed slight reduction of twitching and swimming mobility. Excellent antifungal oil activity, good antioxidant, antimicrobial and anti-quorum activity of L. ochridanum extracts was found. This study shows for the first time anti-guorum sensing activity against Pseudomonas aeruginosa of L. ochridanum, as well as its significant antioxidant potential. This study was conducted due to adventives of usage of natural products as efficient substitution for synthetic agents in treatment of various medical conditions.",
journal = "Journal of Applied Botany and Food Quality",
title = "Laserpitium ochridanum: antioxidant, antimicrobial and anti-quorum sensing activities against Pseudomonas aeruginosa",
volume = "90",
doi = "10.5073/jabfq.2017.090.041",
pages = "330-338"
}

Mileski, K. S., Ćirić, A., Petrović, J., Ristić, M. S., Matevski, V. S., Marin, P. D.,& Džamić, A. M.. (2017). Laserpitium ochridanum: antioxidant, antimicrobial and anti-quorum sensing activities against Pseudomonas aeruginosa. in Journal of Applied Botany and Food Quality, 90, 330-338.
https://doi.org/10.5073/jabfq.2017.090.041

Mileski KS, Ćirić A, Petrović J, Ristić MS, Matevski VS, Marin PD, Džamić AM. Laserpitium ochridanum: antioxidant, antimicrobial and anti-quorum sensing activities against Pseudomonas aeruginosa. in Journal of Applied Botany and Food Quality. 2017;90:330-338.
doi:10.5073/jabfq.2017.090.041 .

Mileski, Ksenija Slave, Ćirić, Ana, Petrović, Jovana, Ristić, Mihailo S, Matevski, Vlado S, Marin, Petar D, Džamić, Ana M., "Laserpitium ochridanum: antioxidant, antimicrobial and anti-quorum sensing activities against Pseudomonas aeruginosa" in Journal of Applied Botany and Food Quality, 90 (2017):330-338,
https://doi.org/10.5073/jabfq.2017.090.041 . .

TY  - JOUR
AU  - Sparks, Jackson T.
AU  - Bohbot, Jonathan D.
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
AU  - Skorić, Marijana
AU  - Mattoo, Autar
AU  - Dickens, Joseph C.
PY  - 2017
UR  - https://academic.oup.com/jme/article-lookup/doi/10.1093/jme/tjx059
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2808
AB  - Nepeta essential oil (Neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. However, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito Aedes aegypti (L.), an important vector of Zika virus, were poorly understood. Here we show that Neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female Ae. aegypti. A gustatory receptor neuron sensitive to the feeding deterrent quinine and housed within sensilla on the labella of females was activated by both Neo and nepetalactone. Activity of a second gustatory receptor neuron sensitive to the feeding stimulant sucrose was suppressed by both repellents. Our results provide neural pathways for the reported spatial repellency and feeding deterrence of these repell ents. A better understanding of the neural input through which female mosquitoes make decisions to feed will facilitate design of new repellents and management strategies involving their use.
T2  - Journal of Medical Entomology
T1  - Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus
DO  - 10.1093/jme/tjx059
ER  - 

@article{
author = "Sparks, Jackson T. and Bohbot, Jonathan D. and Ristić, Mihailo and Mišić, Danijela and Skorić, Marijana and Mattoo, Autar and Dickens, Joseph C.",
year = "2017",
abstract = "Nepeta essential oil (Neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. However, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito Aedes aegypti (L.), an important vector of Zika virus, were poorly understood. Here we show that Neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female Ae. aegypti. A gustatory receptor neuron sensitive to the feeding deterrent quinine and housed within sensilla on the labella of females was activated by both Neo and nepetalactone. Activity of a second gustatory receptor neuron sensitive to the feeding stimulant sucrose was suppressed by both repellents. Our results provide neural pathways for the reported spatial repellency and feeding deterrence of these repell ents. A better understanding of the neural input through which female mosquitoes make decisions to feed will facilitate design of new repellents and management strategies involving their use.",
journal = "Journal of Medical Entomology",
title = "Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus",
doi = "10.1093/jme/tjx059"
}

Sparks, J. T., Bohbot, J. D., Ristić, M., Mišić, D., Skorić, M., Mattoo, A.,& Dickens, J. C.. (2017). Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus. in Journal of Medical Entomology.
https://doi.org/10.1093/jme/tjx059

Sparks JT, Bohbot JD, Ristić M, Mišić D, Skorić M, Mattoo A, Dickens JC. Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus. in Journal of Medical Entomology. 2017;.
doi:10.1093/jme/tjx059 .

Sparks, Jackson T., Bohbot, Jonathan D., Ristić, Mihailo, Mišić, Danijela, Skorić, Marijana, Mattoo, Autar, Dickens, Joseph C., "Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus" in Journal of Medical Entomology (2017),
https://doi.org/10.1093/jme/tjx059 . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Gligorijević, Nevenka
AU  - Čavić, Milena
AU  - Todorović, Slađana
AU  - Janković, Radmila
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
AU  - Radulović, Siniša
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0926669017301309
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2609
AB  - Nepeta rtanjensis Diklić & Milojević (fam. Lamiaceae) is an endemic, critically endangered plant, protected by the law in Serbia. Various biological activities have been ascribed to its major constituents, nepetalactones. In this study we describe for the first time cytotoxic activity of N. rtanjensis essential oil (EO), obtained from field cultivated plants, which was found to be especially rich in trans,cis-nepetalactone. MTT assays indicated that after 72 h of treatment the EO exhibited cytotoxic activity on investigated cancer cell lines: HeLa, K562, A549, LS-174 and MDA-MB-231. Normal cell line (MRC-5) was the least sensitive to the treatment and IC50 value for this cell line was not reached within the tested range of EO concentrations (up to 0.1 μL/mL). Analysis of morphological changes of treated cells confirmed the higher sensitivity of tumor cells than normal cells to the tested EO. Application of N. rtanjensis EO resulted in the appearance of morphological changes in tested cancer cell lines characteristic for apoptotic cell death, and induced perturbations of the cell cycle of HeLa cells. In addition, upregulation of Bax and p53, and downregulation of Bcl-2, and Skp2 genes, involved in apoptotic signalling cascades, confirmed an apoptosis-inducing effect of N. rtanjensis EO on HeLa cells. Presented results highlighted the potential of N. rtanjensis EO in anticancer therapy.
T2  - Industrial Crops and Products
T1  - Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action
VL  - 100
DO  - 10.1016/j.indcrop.2017.02.027
SP  - 163
EP  - 170
ER  - 

@article{
author = "Skorić, Marijana and Gligorijević, Nevenka and Čavić, Milena and Todorović, Slađana and Janković, Radmila and Ristić, Mihailo and Mišić, Danijela and Radulović, Siniša",
year = "2017",
abstract = "Nepeta rtanjensis Diklić & Milojević (fam. Lamiaceae) is an endemic, critically endangered plant, protected by the law in Serbia. Various biological activities have been ascribed to its major constituents, nepetalactones. In this study we describe for the first time cytotoxic activity of N. rtanjensis essential oil (EO), obtained from field cultivated plants, which was found to be especially rich in trans,cis-nepetalactone. MTT assays indicated that after 72 h of treatment the EO exhibited cytotoxic activity on investigated cancer cell lines: HeLa, K562, A549, LS-174 and MDA-MB-231. Normal cell line (MRC-5) was the least sensitive to the treatment and IC50 value for this cell line was not reached within the tested range of EO concentrations (up to 0.1 μL/mL). Analysis of morphological changes of treated cells confirmed the higher sensitivity of tumor cells than normal cells to the tested EO. Application of N. rtanjensis EO resulted in the appearance of morphological changes in tested cancer cell lines characteristic for apoptotic cell death, and induced perturbations of the cell cycle of HeLa cells. In addition, upregulation of Bax and p53, and downregulation of Bcl-2, and Skp2 genes, involved in apoptotic signalling cascades, confirmed an apoptosis-inducing effect of N. rtanjensis EO on HeLa cells. Presented results highlighted the potential of N. rtanjensis EO in anticancer therapy.",
journal = "Industrial Crops and Products",
title = "Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action",
volume = "100",
doi = "10.1016/j.indcrop.2017.02.027",
pages = "163-170"
}

Skorić, M., Gligorijević, N., Čavić, M., Todorović, S., Janković, R., Ristić, M., Mišić, D.,& Radulović, S.. (2017). Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action. in Industrial Crops and Products, 100, 163-170.
https://doi.org/10.1016/j.indcrop.2017.02.027

Skorić M, Gligorijević N, Čavić M, Todorović S, Janković R, Ristić M, Mišić D, Radulović S. Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action. in Industrial Crops and Products. 2017;100:163-170.
doi:10.1016/j.indcrop.2017.02.027 .

Skorić, Marijana, Gligorijević, Nevenka, Čavić, Milena, Todorović, Slađana, Janković, Radmila, Ristić, Mihailo, Mišić, Danijela, Radulović, Siniša, "Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action" in Industrial Crops and Products, 100 (2017):163-170,
https://doi.org/10.1016/j.indcrop.2017.02.027 . .

TY  - JOUR
AU  - Sparks, Jackson T.
AU  - Bohbot, Jonathan D.
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
AU  - Skorić, Marijana
AU  - Mattoo, Autar
AU  - Dickens, Joseph C.
PY  - 2017
UR  - https://academic.oup.com/jme/article-lookup/doi/10.1093/jme/tjx059
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2805
AB  - Nepeta essential oil (Neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. However, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito Aedes aegypti (L.), an important vector of Zika virus, were poorly understood. Here we show that Neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female Ae. aegypti. A gustatory receptor neuron sensitive to the feeding deterrent quinine and housed within sensilla on the labella of females was activated by both Neo and nepetalactone. Activity of a second gustatory receptor neuron sensitive to the feeding stimulant sucrose was suppressed by both repellents. Our results provide neural pathways for the reported spatial repellency and feeding deterrence of these repell ents. A better understanding of the neural input through which female mosquitoes make decisions to feed will facilitate design of new repellents and management strategies involving their use.
T2  - Journal of Medical Entomology
T1  - Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus
IS  - 4
VL  - 54
DO  - 10.1093/jme/tjx059
SP  - 957
EP  - 963
ER  - 

@article{
author = "Sparks, Jackson T. and Bohbot, Jonathan D. and Ristić, Mihailo and Mišić, Danijela and Skorić, Marijana and Mattoo, Autar and Dickens, Joseph C.",
year = "2017",
abstract = "Nepeta essential oil (Neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. However, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito Aedes aegypti (L.), an important vector of Zika virus, were poorly understood. Here we show that Neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female Ae. aegypti. A gustatory receptor neuron sensitive to the feeding deterrent quinine and housed within sensilla on the labella of females was activated by both Neo and nepetalactone. Activity of a second gustatory receptor neuron sensitive to the feeding stimulant sucrose was suppressed by both repellents. Our results provide neural pathways for the reported spatial repellency and feeding deterrence of these repell ents. A better understanding of the neural input through which female mosquitoes make decisions to feed will facilitate design of new repellents and management strategies involving their use.",
journal = "Journal of Medical Entomology",
title = "Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus",
number = "4",
volume = "54",
doi = "10.1093/jme/tjx059",
pages = "957-963"
}

Sparks, J. T., Bohbot, J. D., Ristić, M., Mišić, D., Skorić, M., Mattoo, A.,& Dickens, J. C.. (2017). Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus. in Journal of Medical Entomology, 54(4), 957-963.
https://doi.org/10.1093/jme/tjx059

Sparks JT, Bohbot JD, Ristić M, Mišić D, Skorić M, Mattoo A, Dickens JC. Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus. in Journal of Medical Entomology. 2017;54(4):957-963.
doi:10.1093/jme/tjx059 .

Sparks, Jackson T., Bohbot, Jonathan D., Ristić, Mihailo, Mišić, Danijela, Skorić, Marijana, Mattoo, Autar, Dickens, Joseph C., "Chemosensory Responses to the Repellent Nepeta Essential Oil and Its Major Component Nepetalactone by Aedes aegypti (Diptera: Culicidae), a Vector of Zika Virus" in Journal of Medical Entomology, 54, no. 4 (2017):957-963,
https://doi.org/10.1093/jme/tjx059 . .

TY  - JOUR
AU  - Marković, Milena
AU  - Ristić, Mihailo
AU  - Popović, Zorica
AU  - Matić, Rada
AU  - Nikolić, Biljana
AU  - Vidaković, Vera
AU  - Obratov-Petković, Dragica
AU  - Bojović, Srđan
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3502
AB  - The composition of the essential oil of the twigs and needles of Taxus baccata L. from three natural populations in Serbia has been determined by GC/MS analysis. Of the 91 detected compounds, 87 were identified. The most abundant compound classes were aliphatic alcohols, terpenes, aliphatic hydrocarbons, and aliphatic aldehydes, which together comprised ca. 86.92% of the total oil composition. The dominant constituents were oct‐1‐en‐3‐ol (23.48%), (3Z)‐hex‐3‐en‐1‐ol (11.46%; aliphatic alcohols), and myrtenol (11.38%; oxygenated monoterpene). The PCA of 22 selected compounds revealed differentiations of populations based on geographic distribution. The CA showed that Populations I and II from the Dinaric Alps were similar, and that Population III from the Balkan mountain system was distinct. This was the first investigation of interpopulation variability of T. baccata populations based on essential oil composition. The results of this study were compared with those of previous studies concerning volatile compounds produced by Taxus species. The results indicate that the essential oil content of T. baccata populations from this study is unique, mostly resembling the population from southeast Serbia.
PB  - Zürich: Wiley‐VHCA AG
T2  - Chemistry and Biodiversity
T1  - Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia
IS  - 7
VL  - 13
DO  - 10.1002/cbdv.201500326
SP  - 943
EP  - 953
ER  - 

@article{
author = "Marković, Milena and Ristić, Mihailo and Popović, Zorica and Matić, Rada and Nikolić, Biljana and Vidaković, Vera and Obratov-Petković, Dragica and Bojović, Srđan",
year = "2016",
abstract = "The composition of the essential oil of the twigs and needles of Taxus baccata L. from three natural populations in Serbia has been determined by GC/MS analysis. Of the 91 detected compounds, 87 were identified. The most abundant compound classes were aliphatic alcohols, terpenes, aliphatic hydrocarbons, and aliphatic aldehydes, which together comprised ca. 86.92% of the total oil composition. The dominant constituents were oct‐1‐en‐3‐ol (23.48%), (3Z)‐hex‐3‐en‐1‐ol (11.46%; aliphatic alcohols), and myrtenol (11.38%; oxygenated monoterpene). The PCA of 22 selected compounds revealed differentiations of populations based on geographic distribution. The CA showed that Populations I and II from the Dinaric Alps were similar, and that Population III from the Balkan mountain system was distinct. This was the first investigation of interpopulation variability of T. baccata populations based on essential oil composition. The results of this study were compared with those of previous studies concerning volatile compounds produced by Taxus species. The results indicate that the essential oil content of T. baccata populations from this study is unique, mostly resembling the population from southeast Serbia.",
publisher = "Zürich: Wiley‐VHCA AG",
journal = "Chemistry and Biodiversity",
title = "Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia",
number = "7",
volume = "13",
doi = "10.1002/cbdv.201500326",
pages = "943-953"
}

Marković, M., Ristić, M., Popović, Z., Matić, R., Nikolić, B., Vidaković, V., Obratov-Petković, D.,& Bojović, S.. (2016). Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia. in Chemistry and Biodiversity
Zürich: Wiley‐VHCA AG., 13(7), 943-953.
https://doi.org/10.1002/cbdv.201500326

Marković M, Ristić M, Popović Z, Matić R, Nikolić B, Vidaković V, Obratov-Petković D, Bojović S. Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia. in Chemistry and Biodiversity. 2016;13(7):943-953.
doi:10.1002/cbdv.201500326 .

Marković, Milena, Ristić, Mihailo, Popović, Zorica, Matić, Rada, Nikolić, Biljana, Vidaković, Vera, Obratov-Petković, Dragica, Bojović, Srđan, "Chemical Composition and Interpopulation Variability of Essential Oils of Taxus baccata L. from Serbia" in Chemistry and Biodiversity, 13, no. 7 (2016):943-953,
https://doi.org/10.1002/cbdv.201500326 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ristić, Mihailo
AU  - Perišić, Mirjana
AU  - Puač, Nevena
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6190
AB  - Volatile organic compounds (VOC) emitted by fronds of desiccation tolerant fern Asplenium ceterach L. during the rehydration process were investigated. Head-space GC-MS analysis was used in order to determine the volatile compounds in dry and rehydrated sample of golden fern. It was found that the VOC profile of golden fern is based mainly on isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols and related compounds. Aerial parts of fresh and dry fronds do not contain monoterpene-, sesquiterpene- and diterpene-type hydrocarbons or corresponding terpenoids. In order to determine the composition of the VOC during the process of rehydration, we have applied proton-transfer reaction mass spectrometry (PTR-MS). PTR-MS is a sensitive technique that allows real-time detection of VOCs emitted from plants. We have used dry plants to establish a base line and then distilled water was added to induce hydration process of the golden fern plant. Masses in the range from m/z 21 to m/z 300 were measured with dwell time of 200 ms and for the time period of 24 hours. The experiment was repeated for five plants. PTR-MS measurements revealed that the amounts of compounds, determined by head-space GC-MS, generally exhibit a significant increase after addition of water. After about 20 hours the values measured by PTR-MS for these masses are reduced to a new base line that corresponds to the rehydrated plant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6190
ER  - 

@conference{
author = "Živković, Suzana and Skorić, Marijana and Ristić, Mihailo and Perišić, Mirjana and Puač, Nevena and Malović, Gordana and Petrović, Zoran Lj",
year = "2015",
abstract = "Volatile organic compounds (VOC) emitted by fronds of desiccation tolerant fern Asplenium ceterach L. during the rehydration process were investigated. Head-space GC-MS analysis was used in order to determine the volatile compounds in dry and rehydrated sample of golden fern. It was found that the VOC profile of golden fern is based mainly on isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols and related compounds. Aerial parts of fresh and dry fronds do not contain monoterpene-, sesquiterpene- and diterpene-type hydrocarbons or corresponding terpenoids. In order to determine the composition of the VOC during the process of rehydration, we have applied proton-transfer reaction mass spectrometry (PTR-MS). PTR-MS is a sensitive technique that allows real-time detection of VOCs emitted from plants. We have used dry plants to establish a base line and then distilled water was added to induce hydration process of the golden fern plant. Masses in the range from m/z 21 to m/z 300 were measured with dwell time of 200 ms and for the time period of 24 hours. The experiment was repeated for five plants. PTR-MS measurements revealed that the amounts of compounds, determined by head-space GC-MS, generally exhibit a significant increase after addition of water. After about 20 hours the values measured by PTR-MS for these masses are reduced to a new base line that corresponds to the rehydrated plant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration",
pages = "187",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6190"
}

Živković, S., Skorić, M., Ristić, M., Perišić, M., Puač, N., Malović, G.,& Petrović, Z. L.. (2015). Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 187.
https://hdl.handle.net/21.15107/rcub_ibiss_6190

Živković S, Skorić M, Ristić M, Perišić M, Puač N, Malović G, Petrović ZL. Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:187.
https://hdl.handle.net/21.15107/rcub_ibiss_6190 .

Živković, Suzana, Skorić, Marijana, Ristić, Mihailo, Perišić, Mirjana, Puač, Nevena, Malović, Gordana, Petrović, Zoran Lj, "Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):187,
https://hdl.handle.net/21.15107/rcub_ibiss_6190 .

TY  - CONF
AU  - Dmitrović, Slavica
AU  - Perišić, Mirjana
AU  - Stojić, Andreja
AU  - Živković, Suzana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Aničić, Neda
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6185
AB  - The possibility of using essential oil (EO) of Nepeta rtanjensis and N. cataria as bioherbicides against Ambrosia artemisiifolia L., an important weed and allergen species, was explored. In vitro culture experimental system of A. artemisiifolia has been established and ragweed shoots were exposed to the atmosphere enriched with volatile compounds (VOCs) emitted from EOs. N. rtanjensis EO was characterized by high amounts of 4aα,7α,7aβ-nepetalactone (trans,cis-nepetalactone), while N. cataria EO possessed high amounts of 4aα,7α,7aα-nepetalactone (cis,trans-nepetalactone). Reduction in A. artemisiifolia shoots growth and their discoloration, in the presence of N. rtanjensis and N. cataria EOs (2% and 4%, final nepetalactone concentrations) was observed after 2 weeks of exposure. An antioxidative defense system of A. artemisiifolia was activated by increasing peroxidases activity or by inactivating catalases and superoxide dismutases activity. Overall, stronger inhibitory effect on shoot growth, CAT activity, and stimulating POX activity was observed for N. cataria EO. N. rtanjensis EO was more efficient in inhibiting rooting and root growth, and in suppressing SOD activity. Therefore, EOs of N. rtanjensis and N. cataria might be recommended as potential bioherbicides against highly allergenic
and weed species A. artemisiifolia. Further exploration of EOs’ mode of action, confirmation of phytotoxicity in field conditions, and feasibility assessment for the commercial production and application in agricultural practice, is the course of our further work.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils
SP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6185
ER  - 

@conference{
author = "Dmitrović, Slavica and Perišić, Mirjana and Stojić, Andreja and Živković, Suzana and Božunović, Jelena and Nestorović Živković, Jasmina and Aničić, Neda and Ristić, Mihailo and Mišić, Danijela",
year = "2015",
abstract = "The possibility of using essential oil (EO) of Nepeta rtanjensis and N. cataria as bioherbicides against Ambrosia artemisiifolia L., an important weed and allergen species, was explored. In vitro culture experimental system of A. artemisiifolia has been established and ragweed shoots were exposed to the atmosphere enriched with volatile compounds (VOCs) emitted from EOs. N. rtanjensis EO was characterized by high amounts of 4aα,7α,7aβ-nepetalactone (trans,cis-nepetalactone), while N. cataria EO possessed high amounts of 4aα,7α,7aα-nepetalactone (cis,trans-nepetalactone). Reduction in A. artemisiifolia shoots growth and their discoloration, in the presence of N. rtanjensis and N. cataria EOs (2% and 4%, final nepetalactone concentrations) was observed after 2 weeks of exposure. An antioxidative defense system of A. artemisiifolia was activated by increasing peroxidases activity or by inactivating catalases and superoxide dismutases activity. Overall, stronger inhibitory effect on shoot growth, CAT activity, and stimulating POX activity was observed for N. cataria EO. N. rtanjensis EO was more efficient in inhibiting rooting and root growth, and in suppressing SOD activity. Therefore, EOs of N. rtanjensis and N. cataria might be recommended as potential bioherbicides against highly allergenic
and weed species A. artemisiifolia. Further exploration of EOs’ mode of action, confirmation of phytotoxicity in field conditions, and feasibility assessment for the commercial production and application in agricultural practice, is the course of our further work.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils",
pages = "159",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6185"
}

Dmitrović, S., Perišić, M., Stojić, A., Živković, S., Božunović, J., Nestorović Živković, J., Aničić, N., Ristić, M.,& Mišić, D.. (2015). The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 159.
https://hdl.handle.net/21.15107/rcub_ibiss_6185

Dmitrović S, Perišić M, Stojić A, Živković S, Božunović J, Nestorović Živković J, Aničić N, Ristić M, Mišić D. The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:159.
https://hdl.handle.net/21.15107/rcub_ibiss_6185 .

Dmitrović, Slavica, Perišić, Mirjana, Stojić, Andreja, Živković, Suzana, Božunović, Jelena, Nestorović Živković, Jasmina, Aničić, Neda, Ristić, Mihailo, Mišić, Danijela, "The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):159,
https://hdl.handle.net/21.15107/rcub_ibiss_6185 .

TY  - CONF
AU  - Skorić, Marijana
AU  - Todorović, Slađana
AU  - Ristić, Mihailo
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Živković, Suzana
AU  - Stojić, Andreja
AU  - Puač, Nevena
AU  - Kanellis, Angelos K
PY  - 2013
UR  - http://terpnet2013.pharm.auth.gr/site/TERPNET_BOOK_OF_ABSTRACTS.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6320
AB  - Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.
PB  - Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki
C3  - TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
T1  - In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6320
ER  - 

@conference{
author = "Skorić, Marijana and Todorović, Slađana and Ristić, Mihailo and Soković, Marina and Glamočlija, Jasmina and Živković, Suzana and Stojić, Andreja and Puač, Nevena and Kanellis, Angelos K",
year = "2013",
abstract = "Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.",
publisher = "Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki",
journal = "TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece",
title = "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds",
pages = "132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6320"
}

Skorić, M., Todorović, S., Ristić, M., Soković, M., Glamočlija, J., Živković, S., Stojić, A., Puač, N.,& Kanellis, A. K.. (2013). In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki., 132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320

Skorić M, Todorović S, Ristić M, Soković M, Glamočlija J, Živković S, Stojić A, Puač N, Kanellis AK. In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece. 2013;:132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .

Skorić, Marijana, Todorović, Slađana, Ristić, Mihailo, Soković, Marina, Glamočlija, Jasmina, Živković, Suzana, Stojić, Andreja, Puač, Nevena, Kanellis, Angelos K, "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds" in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece (2013):132,
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .

TY  - CONF
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Jovanović, Vladan
AU  - Šiler, Branislav
AU  - Ristić, Mihailo
AU  - Cvetković, Jelena
AU  - Živković, Suzana
AU  - Mišić, Danijela
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6397
AB  - Allelopathy, the chemical mechanism of plant interference, was detected in many plants from fam. Lamiaceae, including Nepeta species. The majority of Nepeta species are characterized by the presence of ne­petalactone as the dominant component in essential oils. This monoterpenoid lactone exists in the form of one or more stereoisomers, and corresponding enantiomers. The present study investigates the allelopathic potential of Nepeta rtanjensís Diklić & Milojević and Nepeta ca­taria L. essential oils which differ in their qualitative nepetalactone content, on the seed germination of four selected crop species (Lepidium sativum L., Lactuca sativa L. cv. Majska Kraljica, Lotus corniculatus L., cv. Bokor and Brassica napus L. cv. napus). GC-MS analysis revealed that trans,cis- stereoisomer of nepetalactone is dom­inant in essential oil of N. rtanjensis (72,03%). Essential oil of N. cataria used in the present study is character­ized by the high content of cís, trans-nepetalactone (90%). Both essential oils exhibited significant allelopath­ic potential against all model species, with N. rtanjensís essential oil being more efficient. These results sug­gest that nepetalactone stereochemistry significantly determines its allelopathic potential, and that the ef­fect of both nepetalactone stereoisomers is dose-dependent. Essential oils reduced seed germination by in­fluencing germination dynamics, or by irreversibly inhibiting the germination of seeds. Among tested crops, L. sativa was shown to be the most sensitive to allelopathic potential of N. rtanjensis and N. cataria essential oils, while B. napus was the most tolerant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6397
ER  - 

@conference{
author = "Nestorović Živković, Jasmina and Dmitrović, Slavica and Jovanović, Vladan and Šiler, Branislav and Ristić, Mihailo and Cvetković, Jelena and Živković, Suzana and Mišić, Danijela",
year = "2013",
abstract = "Allelopathy, the chemical mechanism of plant interference, was detected in many plants from fam. Lamiaceae, including Nepeta species. The majority of Nepeta species are characterized by the presence of ne­petalactone as the dominant component in essential oils. This monoterpenoid lactone exists in the form of one or more stereoisomers, and corresponding enantiomers. The present study investigates the allelopathic potential of Nepeta rtanjensís Diklić & Milojević and Nepeta ca­taria L. essential oils which differ in their qualitative nepetalactone content, on the seed germination of four selected crop species (Lepidium sativum L., Lactuca sativa L. cv. Majska Kraljica, Lotus corniculatus L., cv. Bokor and Brassica napus L. cv. napus). GC-MS analysis revealed that trans,cis- stereoisomer of nepetalactone is dom­inant in essential oil of N. rtanjensis (72,03%). Essential oil of N. cataria used in the present study is character­ized by the high content of cís, trans-nepetalactone (90%). Both essential oils exhibited significant allelopath­ic potential against all model species, with N. rtanjensís essential oil being more efficient. These results sug­gest that nepetalactone stereochemistry significantly determines its allelopathic potential, and that the ef­fect of both nepetalactone stereoisomers is dose-dependent. Essential oils reduced seed germination by in­fluencing germination dynamics, or by irreversibly inhibiting the germination of seeds. Among tested crops, L. sativa was shown to be the most sensitive to allelopathic potential of N. rtanjensis and N. cataria essential oils, while B. napus was the most tolerant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6397"
}

Nestorović Živković, J., Dmitrović, S., Jovanović, V., Šiler, B., Ristić, M., Cvetković, J., Živković, S.,& Mišić, D.. (2013). Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_6397

Nestorović Živković J, Dmitrović S, Jovanović V, Šiler B, Ristić M, Cvetković J, Živković S, Mišić D. Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_6397 .

Nestorović Živković, Jasmina, Dmitrović, Slavica, Jovanović, Vladan, Šiler, Branislav, Ristić, Mihailo, Cvetković, Jelena, Živković, Suzana, Mišić, Danijela, "Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):81,
https://hdl.handle.net/21.15107/rcub_ibiss_6397 .

TY  - JOUR
AU  - Grbić-Ljaljević, Milica
AU  - Soković, Marina
AU  - Stupar, Miloš
AU  - Mišić, Danijela
AU  - Vukojević, Jelena
AU  - Grubišić, Dragoljub
AU  - Ristić, Mihailo
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/556
AB  - The chemical composition and antifungal activity of the essential oil of an endemic Serbian plant Nepeta rtanjensis Diklić & Milojević was studied. The essential oil was isolated from cultivated plants. Inhibition of mycelia growth of five micromycetes, two Alternaria species originally isolated from N. rtanjensis, Cladosporium cladosporoides, Trichoderma viride and Bipolaris spicifera, were tested using the agar dilution method. The essential oil of N. rtanjensis, the main component of which was 4aα,7α,7αβ-nepetalactone, showed strong antifungal activity against all the tested micromycetes. The minimum inhibitory concentration of N. rtanjensis essential oil ranged from 0.6 to 1.4 μg mL-1. The fungi most sensitive to the tested oil were Alternaria species, while Trichoderma viride was the most resistant.
AB  - U ovom radu prezentovan je hemijski sastav i antifungalna aktivnost etarskog ulja endemične biljke Nepeta rtanjensis Diklić & Milojević. Etarsko ulje je izolovano iz kultivisanih biljaka. Inhibicija micelijalnog rasta pet mikromiceta, dve vrste roda Alternaria, izolovane sa N. rtanjensis, Cladosporium cladosporioides, Trichoderma viride i Bipolaris spicifera, testirana je makrodilucionom metodom. Etarsko ulje N. rtanjensis, čija je glavna komponenta 4aα,7α,7aβ-nepetalakton pokazuje jaku antifungalnu aktivnost u odnosu na sve testirane mikromicete. Minimalna inhibitorna koncentracija (MIC) etaskog ulja bila je u rasponu od 0,6 μg mL-1 do 1,4 μg mL-1. Najveću osetljivost na testirano ulje pokazale su vrste roda Alternaria dok je Trichoderma viride bila najotpornija.
T2  - Journal of the Serbian Chemical Society
T1  - Antifungalna aktivnost etarskog ulja
T1  - Antifungal activity of Nepeta rtanjensis essential oil
IS  - 10
VL  - 73
SP  - 961
EP  - 965
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_556
ER  - 

@article{
author = "Grbić-Ljaljević, Milica and Soković, Marina and Stupar, Miloš and Mišić, Danijela and Vukojević, Jelena and Grubišić, Dragoljub and Ristić, Mihailo",
year = "2008, 2008",
abstract = "The chemical composition and antifungal activity of the essential oil of an endemic Serbian plant Nepeta rtanjensis Diklić & Milojević was studied. The essential oil was isolated from cultivated plants. Inhibition of mycelia growth of five micromycetes, two Alternaria species originally isolated from N. rtanjensis, Cladosporium cladosporoides, Trichoderma viride and Bipolaris spicifera, were tested using the agar dilution method. The essential oil of N. rtanjensis, the main component of which was 4aα,7α,7αβ-nepetalactone, showed strong antifungal activity against all the tested micromycetes. The minimum inhibitory concentration of N. rtanjensis essential oil ranged from 0.6 to 1.4 μg mL-1. The fungi most sensitive to the tested oil were Alternaria species, while Trichoderma viride was the most resistant., U ovom radu prezentovan je hemijski sastav i antifungalna aktivnost etarskog ulja endemične biljke Nepeta rtanjensis Diklić & Milojević. Etarsko ulje je izolovano iz kultivisanih biljaka. Inhibicija micelijalnog rasta pet mikromiceta, dve vrste roda Alternaria, izolovane sa N. rtanjensis, Cladosporium cladosporioides, Trichoderma viride i Bipolaris spicifera, testirana je makrodilucionom metodom. Etarsko ulje N. rtanjensis, čija je glavna komponenta 4aα,7α,7aβ-nepetalakton pokazuje jaku antifungalnu aktivnost u odnosu na sve testirane mikromicete. Minimalna inhibitorna koncentracija (MIC) etaskog ulja bila je u rasponu od 0,6 μg mL-1 do 1,4 μg mL-1. Najveću osetljivost na testirano ulje pokazale su vrste roda Alternaria dok je Trichoderma viride bila najotpornija.",
journal = "Journal of the Serbian Chemical Society",
title = "Antifungalna aktivnost etarskog ulja, Antifungal activity of Nepeta rtanjensis essential oil",
number = "10",
volume = "73",
pages = "961-965",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_556"
}

Grbić-Ljaljević, M., Soković, M., Stupar, M., Mišić, D., Vukojević, J., Grubišić, D.,& Ristić, M.. (2008). Antifungalna aktivnost etarskog ulja. in Journal of the Serbian Chemical Society, 73(10), 961-965.
https://hdl.handle.net/21.15107/rcub_ibiss_556

Grbić-Ljaljević M, Soković M, Stupar M, Mišić D, Vukojević J, Grubišić D, Ristić M. Antifungalna aktivnost etarskog ulja. in Journal of the Serbian Chemical Society. 2008;73(10):961-965.
https://hdl.handle.net/21.15107/rcub_ibiss_556 .

Grbić-Ljaljević, Milica, Soković, Marina, Stupar, Miloš, Mišić, Danijela, Vukojević, Jelena, Grubišić, Dragoljub, Ristić, Mihailo, "Antifungalna aktivnost etarskog ulja" in Journal of the Serbian Chemical Society, 73, no. 10 (2008):961-965,
https://hdl.handle.net/21.15107/rcub_ibiss_556 .

TY  - JOUR
AU  - Ljaljević-Grbić, Milica V.
AU  - Soković, Marina
AU  - Vukojević, Jelena B.
AU  - Grubišić, Dragoljub
AU  - Ristić, Mihailo
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/537
AB  - The antifungal activity of Nepeta rtanjensis Diklić et Milojević essential oil on mycelia growth has been performed by macrodilution method. The most efficient impact of N. rtanjensis essential oil on mycelia growth in vitro was found in Alternaria species with the same value of minimal inhibitory quantity (MIQ) of 0.6 μl/ml. Bipolaris spicifera and Cladosporium cladosporoioides had MIQ values of 1.0 μl/ml whereas Trichoderma viride with MIQ value of 1.6 μl/ml showed the most efficient defense against the essential oil examined. The values of minimal fungicidal quantity (MFQ) in Alternaria sp. 2, B. spicifera and C. cladosporioides match the MIQ values whereas MFQ values in Alternaria sp. 1 is 0.8 μl/ml, and in T. viride 1.8 μl/ml.
AB  - Makrodilucionom metodom testiran je uticaj etarskog ulja endemične biljke Nepeta rtanjensis (Lamiaceae) na micelijalni rast mikromiceta: Alternaria sp. 1, Alternaria sp. 2, Bipolaris spicifera, Cladosporium cladosporioides i Trichoderma viride. Hemijska analiza etarskog ulja N. rtanjensis pokazala je apsolutnu dominaciju 4aα, 7α, 7aβ nepetalaktona (79.89%). Najefikasniji uticaj na rast micelije in vitro zabeležen je kod vrsta roda Alternaria sa istom vrednošću minimalne inhibitorne količine (MIK) od 0.6 μl/ml. Bipolaris spicifera i Cladosporium cladosporioides su imali MIK 1.0 μl/ml, dok je Trichoderma viride, sa vrednošću MIK od 1.6 μl/ml, pokazala najveću otpornost na dejstvo ispitivanog ulja. Vrednosti minimalne fungicidne količine (MFK) se kod Alternaria sp. 2 (0.6 μl/ml), B. spicifera (1.0 μl/ml) i C. cladosporioides (1.0 μl/ml) poklapaju sa vrednostima MIK, dok je kod Alternaria sp. 1 MFK 0.8 μl/ml, a kod T. viride 1.8 μl/ml. Etarsko ulje N. rtanjensis pokazalo je jako antifungalno dejstvo na rast micelije testiranih mikromiceta.
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta
T1  - The effect of Nepeta rtanjensis essential oil on test micromycetes mycelia growth
IS  - 113
VL  - null
SP  - 173
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_537
ER  - 

@article{
author = "Ljaljević-Grbić, Milica V. and Soković, Marina and Vukojević, Jelena B. and Grubišić, Dragoljub and Ristić, Mihailo",
year = "2007, 2007",
abstract = "The antifungal activity of Nepeta rtanjensis Diklić et Milojević essential oil on mycelia growth has been performed by macrodilution method. The most efficient impact of N. rtanjensis essential oil on mycelia growth in vitro was found in Alternaria species with the same value of minimal inhibitory quantity (MIQ) of 0.6 μl/ml. Bipolaris spicifera and Cladosporium cladosporoioides had MIQ values of 1.0 μl/ml whereas Trichoderma viride with MIQ value of 1.6 μl/ml showed the most efficient defense against the essential oil examined. The values of minimal fungicidal quantity (MFQ) in Alternaria sp. 2, B. spicifera and C. cladosporioides match the MIQ values whereas MFQ values in Alternaria sp. 1 is 0.8 μl/ml, and in T. viride 1.8 μl/ml., Makrodilucionom metodom testiran je uticaj etarskog ulja endemične biljke Nepeta rtanjensis (Lamiaceae) na micelijalni rast mikromiceta: Alternaria sp. 1, Alternaria sp. 2, Bipolaris spicifera, Cladosporium cladosporioides i Trichoderma viride. Hemijska analiza etarskog ulja N. rtanjensis pokazala je apsolutnu dominaciju 4aα, 7α, 7aβ nepetalaktona (79.89%). Najefikasniji uticaj na rast micelije in vitro zabeležen je kod vrsta roda Alternaria sa istom vrednošću minimalne inhibitorne količine (MIK) od 0.6 μl/ml. Bipolaris spicifera i Cladosporium cladosporioides su imali MIK 1.0 μl/ml, dok je Trichoderma viride, sa vrednošću MIK od 1.6 μl/ml, pokazala najveću otpornost na dejstvo ispitivanog ulja. Vrednosti minimalne fungicidne količine (MFK) se kod Alternaria sp. 2 (0.6 μl/ml), B. spicifera (1.0 μl/ml) i C. cladosporioides (1.0 μl/ml) poklapaju sa vrednostima MIK, dok je kod Alternaria sp. 1 MFK 0.8 μl/ml, a kod T. viride 1.8 μl/ml. Etarsko ulje N. rtanjensis pokazalo je jako antifungalno dejstvo na rast micelije testiranih mikromiceta.",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta, The effect of Nepeta rtanjensis essential oil on test micromycetes mycelia growth",
number = "113",
volume = "null",
pages = "173-177",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_537"
}

Ljaljević-Grbić, M. V., Soković, M., Vukojević, J. B., Grubišić, D.,& Ristić, M.. (2007). Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta. in Zbornik Matice srpske za prirodne nauke, null(113), 173-177.
https://hdl.handle.net/21.15107/rcub_ibiss_537

Ljaljević-Grbić MV, Soković M, Vukojević JB, Grubišić D, Ristić M. Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta. in Zbornik Matice srpske za prirodne nauke. 2007;null(113):173-177.
https://hdl.handle.net/21.15107/rcub_ibiss_537 .

Ljaljević-Grbić, Milica V., Soković, Marina, Vukojević, Jelena B., Grubišić, Dragoljub, Ristić, Mihailo, "Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta" in Zbornik Matice srpske za prirodne nauke, null, no. 113 (2007):173-177,
https://hdl.handle.net/21.15107/rcub_ibiss_537 .

TY  - JOUR
AU  - Soković, Marina
AU  - Marin, Petar
AU  - Vukojević, Jelena
AU  - Ristić, Mihailo
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
PY  - 2006
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/408
AB  - Chemical composition and antifungal activities of essential oil of Hyssopus officinalis L. and its component β-pinene were done in this paper using macrodilution and microdilution methods. Minimum inhibitory and minimum fungicidal concentrations (MIC and MFC) in ethanol and Tween were determined. Oil and component were investigated against 12 phyto-, animal- and human pathogens; Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Alternaria alternata, Penicillium ochrochloron, P. funiculosum, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum, Phomopsis helianthi. In essential oil of H. officinalis 27 components were identified (91.70% of total oil). The major compounds were isopinocamphone (43.29%), trans-pinocamphone (16.79%) and β-pinene (16.31%). The essential oil showed strong antifungal activity, with MIC of 2.5 to 3.5 ml/ml in ethanol and Tween. MIC were almost the same in micro- and macrodilution methods, except for Penicillium species where oil dissolved in Tween showed lower MIC. MFC were the same as in macrodilution method. In comparation with bifonazole, essential oil showed much better antifungal potential. .
AB  - U radu je anliziran hemijski sastav etarskog ulja vrste Hyssopus officinalis i antifungalna aktivnost ukupnog ulja i dominantne komponente β-pinena korišćenjem mikro- i makrodilucione metode. Određivane su minimalne inhibitorne i minimalne fungicidne koncentracije (MIC i MFC) u etanolu i Tween-u. Ispitivana jedinjenja testirana su na 12 vrsta biljnih, životinjskih i humanih patogena: Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Alternaria alternata, Penicillium ochrochloron, P. funiculosum, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum, Phomopsis helianthi. U etarskom ulju izopa identifikovano je 27 komponenti što predstavlja 91.70% ulja. Dominantne komponente su izopinokamfon (43,29%), trans-pinokamfon (16,79%) i β-pinen (16,31%). Etarsko ulje izopa je pokazalo dobru antifungalnu aktivnost, sa MIC od 2,5 do 3,5 ml/ml u etanolu i Tween-u. U mikrodilucionoj metodi MIC su uglavnom iste kao i u makrodilucionoj, sem za vrste roda Penicillium, kada je ulje rastvoreno u Tween-u (MIC su manje za 0,5 ml/ml). MFC su iste kao MIC u makrodilucionoj metodi. U odnosu na bifonazol, etarsko ulje izopa poseduje veoma jak antifungalni potencijal. .
T2  - Lekovite sirovine
T1  - Hemijski sastav etarskog ulja Hyssopus officinalis L. i antifungalna aktivnost
T1  - Chemical composition of Hyssopus officinalis L. essential oil and its antifungal activity
IS  - 26-27
VL  - null
SP  - 41
EP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_408
ER  - 

@article{
author = "Soković, Marina and Marin, Petar and Vukojević, Jelena and Ristić, Mihailo and Glamočlija, Jasmina and Ćirić, Ana",
year = "2006, 2006",
abstract = "Chemical composition and antifungal activities of essential oil of Hyssopus officinalis L. and its component β-pinene were done in this paper using macrodilution and microdilution methods. Minimum inhibitory and minimum fungicidal concentrations (MIC and MFC) in ethanol and Tween were determined. Oil and component were investigated against 12 phyto-, animal- and human pathogens; Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Alternaria alternata, Penicillium ochrochloron, P. funiculosum, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum, Phomopsis helianthi. In essential oil of H. officinalis 27 components were identified (91.70% of total oil). The major compounds were isopinocamphone (43.29%), trans-pinocamphone (16.79%) and β-pinene (16.31%). The essential oil showed strong antifungal activity, with MIC of 2.5 to 3.5 ml/ml in ethanol and Tween. MIC were almost the same in micro- and macrodilution methods, except for Penicillium species where oil dissolved in Tween showed lower MIC. MFC were the same as in macrodilution method. In comparation with bifonazole, essential oil showed much better antifungal potential. ., U radu je anliziran hemijski sastav etarskog ulja vrste Hyssopus officinalis i antifungalna aktivnost ukupnog ulja i dominantne komponente β-pinena korišćenjem mikro- i makrodilucione metode. Određivane su minimalne inhibitorne i minimalne fungicidne koncentracije (MIC i MFC) u etanolu i Tween-u. Ispitivana jedinjenja testirana su na 12 vrsta biljnih, životinjskih i humanih patogena: Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Alternaria alternata, Penicillium ochrochloron, P. funiculosum, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum, Phomopsis helianthi. U etarskom ulju izopa identifikovano je 27 komponenti što predstavlja 91.70% ulja. Dominantne komponente su izopinokamfon (43,29%), trans-pinokamfon (16,79%) i β-pinen (16,31%). Etarsko ulje izopa je pokazalo dobru antifungalnu aktivnost, sa MIC od 2,5 do 3,5 ml/ml u etanolu i Tween-u. U mikrodilucionoj metodi MIC su uglavnom iste kao i u makrodilucionoj, sem za vrste roda Penicillium, kada je ulje rastvoreno u Tween-u (MIC su manje za 0,5 ml/ml). MFC su iste kao MIC u makrodilucionoj metodi. U odnosu na bifonazol, etarsko ulje izopa poseduje veoma jak antifungalni potencijal. .",
journal = "Lekovite sirovine",
title = "Hemijski sastav etarskog ulja Hyssopus officinalis L. i antifungalna aktivnost, Chemical composition of Hyssopus officinalis L. essential oil and its antifungal activity",
number = "26-27",
volume = "null",
pages = "41-50",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_408"
}

Soković, M., Marin, P., Vukojević, J., Ristić, M., Glamočlija, J.,& Ćirić, A.. (2006). Hemijski sastav etarskog ulja Hyssopus officinalis L. i antifungalna aktivnost. in Lekovite sirovine, null(26-27), 41-50.
https://hdl.handle.net/21.15107/rcub_ibiss_408

Soković M, Marin P, Vukojević J, Ristić M, Glamočlija J, Ćirić A. Hemijski sastav etarskog ulja Hyssopus officinalis L. i antifungalna aktivnost. in Lekovite sirovine. 2006;null(26-27):41-50.
https://hdl.handle.net/21.15107/rcub_ibiss_408 .

Soković, Marina, Marin, Petar, Vukojević, Jelena, Ristić, Mihailo, Glamočlija, Jasmina, Ćirić, Ana, "Hemijski sastav etarskog ulja Hyssopus officinalis L. i antifungalna aktivnost" in Lekovite sirovine, null, no. 26-27 (2006):41-50,
https://hdl.handle.net/21.15107/rcub_ibiss_408 .