TY  - JOUR
AU  - Sinan, Kouadio Ibrahime
AU  - Ferrarese, Irene
AU  - Aktumsek, Abdurrahman
AU  - Peron, Gregorio
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Nenadić, Marija
AU  - Dall'Acqua, Stefano
AU  - Zengin, Gokhan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4292
AB  - The methanolic and water extracts of Carapa procera leaves and stem barks were screened for their phytochemical content using a multi-technique approach. The extracts were also assessed for their in vitro antioxidant capacity along with their anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase, anti-elastase and anti-cholinesterase (AChE, BChE) activities. Furthermore, antibacterial and antifungal effects were determined against several bacterial and fungal strains. Data Integration Analysis for Biomarker discovery using Latent components (DIABLO) integrative analysis was conducted on collected data to determine the influence of extraction solvents and plant parts on phytochemical content, antioxidant properties and enzyme inhibitory properties of C. procera samples. Additionally, the major identified compounds were screened as modulators of multiple pathways involved in human diseases via Gene Ontology (GO) enrichment analysis. Results showed that methanolic stem bark extract exhibited the most potent ABTS scavenging, Cu2+ and Fe3+ reducing power, total antioxidant capacity and Fe2+ chelating power and displayed the highest total flavanol content. Methanolic extracts of leaves and stem barks were abounded with phenolics and had the greatest anti-AChE, anti-BChE, anti-tyrosinase and anti-elastase activities. A significant antifungal activity was observed, with the lowest minimum inhibitory concentration and minimum fungicidal values of 0.07 and 0.15 mg/mL, respectively. DIABLO integrative analysis suggested that the phytochemical content and biological activities varied significantly within the plant parts and were influenced by types of solvent used. GO enrichment analysis on the main bioactive compounds showed modulation of multiple pathways associated with cancer. Obtained results demonstrated that stem bark and leaves of C. procera can be considered as promising sources of bioactive molecules with high pharmacological values.
PB  - Elsevier
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - NMR and LC-MSn coupled with pharmacological network analysis for the assessment of phytochemical content and biopharmaceutical potential of Carapa procera extracts
VL  - 203
DO  - 10.1016/J.JPBA.2021.114184
SP  - 114184
ER  - 

@article{
author = "Sinan, Kouadio Ibrahime and Ferrarese, Irene and Aktumsek, Abdurrahman and Peron, Gregorio and Glamočlija, Jasmina and Soković, Marina and Nenadić, Marija and Dall'Acqua, Stefano and Zengin, Gokhan",
year = "2021",
abstract = "The methanolic and water extracts of Carapa procera leaves and stem barks were screened for their phytochemical content using a multi-technique approach. The extracts were also assessed for their in vitro antioxidant capacity along with their anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase, anti-elastase and anti-cholinesterase (AChE, BChE) activities. Furthermore, antibacterial and antifungal effects were determined against several bacterial and fungal strains. Data Integration Analysis for Biomarker discovery using Latent components (DIABLO) integrative analysis was conducted on collected data to determine the influence of extraction solvents and plant parts on phytochemical content, antioxidant properties and enzyme inhibitory properties of C. procera samples. Additionally, the major identified compounds were screened as modulators of multiple pathways involved in human diseases via Gene Ontology (GO) enrichment analysis. Results showed that methanolic stem bark extract exhibited the most potent ABTS scavenging, Cu2+ and Fe3+ reducing power, total antioxidant capacity and Fe2+ chelating power and displayed the highest total flavanol content. Methanolic extracts of leaves and stem barks were abounded with phenolics and had the greatest anti-AChE, anti-BChE, anti-tyrosinase and anti-elastase activities. A significant antifungal activity was observed, with the lowest minimum inhibitory concentration and minimum fungicidal values of 0.07 and 0.15 mg/mL, respectively. DIABLO integrative analysis suggested that the phytochemical content and biological activities varied significantly within the plant parts and were influenced by types of solvent used. GO enrichment analysis on the main bioactive compounds showed modulation of multiple pathways associated with cancer. Obtained results demonstrated that stem bark and leaves of C. procera can be considered as promising sources of bioactive molecules with high pharmacological values.",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "NMR and LC-MSn coupled with pharmacological network analysis for the assessment of phytochemical content and biopharmaceutical potential of Carapa procera extracts",
volume = "203",
doi = "10.1016/J.JPBA.2021.114184",
pages = "114184"
}

Sinan, K. I., Ferrarese, I., Aktumsek, A., Peron, G., Glamočlija, J., Soković, M., Nenadić, M., Dall'Acqua, S.,& Zengin, G.. (2021). NMR and LC-MSn coupled with pharmacological network analysis for the assessment of phytochemical content and biopharmaceutical potential of Carapa procera extracts. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier., 203, 114184.
https://doi.org/10.1016/J.JPBA.2021.114184

Sinan KI, Ferrarese I, Aktumsek A, Peron G, Glamočlija J, Soković M, Nenadić M, Dall'Acqua S, Zengin G. NMR and LC-MSn coupled with pharmacological network analysis for the assessment of phytochemical content and biopharmaceutical potential of Carapa procera extracts. in Journal of Pharmaceutical and Biomedical Analysis. 2021;203:114184.
doi:10.1016/J.JPBA.2021.114184 .

Sinan, Kouadio Ibrahime, Ferrarese, Irene, Aktumsek, Abdurrahman, Peron, Gregorio, Glamočlija, Jasmina, Soković, Marina, Nenadić, Marija, Dall'Acqua, Stefano, Zengin, Gokhan, "NMR and LC-MSn coupled with pharmacological network analysis for the assessment of phytochemical content and biopharmaceutical potential of Carapa procera extracts" in Journal of Pharmaceutical and Biomedical Analysis, 203 (2021):114184,
https://doi.org/10.1016/J.JPBA.2021.114184 . .

TY  - JOUR
AU  - Sinan, Kouadio Ibrahime
AU  - Aktumsek, Abdurrahman
AU  - Cádiz-Gurrea, María de la Luz
AU  - Leyva-Jiménez, Francisco Javier
AU  - Fernández-Ochoa, Álvaro
AU  - Segura-Carretero, Antonio
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Nenadic, Marija
AU  - Zengin, Gokhan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4295
AB  - The article reports the chemical composition, antioxidant, six key enzymes inhibitory and antimicrobial
activities of two solvent extracts (water and methanol) of leaves and stem bark of Uapaca togoensis. For chemical composition, methanol extract of stem bark exhibited significant higher total phenolic (129.86 mg GAE/g) and flavanol (10.44 mg CE/g) contents. Methanol extract of leaves and water extract of stem bark showed high flavonoids (20.94 mg RE/g) and phenolic acid (90.40 mg CAE/g) content, respectively. In addition, HPLC-ESI-TOFMS analysis revealed that U. togoensis was rich in procyanidins. The methanol and water extracts of stem bark had overall superior antioxidant activity; however, only methanol extract of stem bark showed higher inhibition of cholinesterase (AChE: 2.57 mg GALAE/g; BChE: 4.69 mg GALAE/g), tyrosinase (69.53 mg KAE/g) and elastase (2.73 mmol CE/g). Potent metal chelating ability was showed by water extract of leaves (18.94 mg EDTAE/g), higher inhibition of amylase was detected for water extracts of leaves (0.94 mmol ACAE/g) and stem bark (0.92 mmol ACAE/g). The tested extracts have shown wide-spectrum antibacterial properties and these effects have shown to be more effective against Aspergillus ochraceus, Penicillium funiculosum, Trichoderma viride, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The results revealed that the antioxidant, enzyme inhibitory and antimicrobial activities depended on the extraction solvents and the parts of plant. Bioinformatics analysis on the 17 major compounds showed modulation of pathway associated with cancer. In brief, U. togoensis might be valuable as potential source of natural agents for therapeutic application.
PB  - Zurich, Switzerland: Wiley-VHCA AG
T2  - Chemistry &  Biodiversity
T1  - A Prospective of Multiple Biopharmaceutical Activities of Procyanidins-Rich Uapaca togoensis Pax Extracts: HPLC-ESI-TOF-MS Coupled with Bioinformatics Analysis
IS  - 8
VL  - 18
DO  - 10.1002/cbdv.202100299
SP  - e2100299
ER  - 

@article{
author = "Sinan, Kouadio Ibrahime and Aktumsek, Abdurrahman and Cádiz-Gurrea, María de la Luz and Leyva-Jiménez, Francisco Javier and Fernández-Ochoa, Álvaro and Segura-Carretero, Antonio and Glamočlija, Jasmina and Soković, Marina and Nenadic, Marija and Zengin, Gokhan",
year = "2021",
abstract = "The article reports the chemical composition, antioxidant, six key enzymes inhibitory and antimicrobial
activities of two solvent extracts (water and methanol) of leaves and stem bark of Uapaca togoensis. For chemical composition, methanol extract of stem bark exhibited significant higher total phenolic (129.86 mg GAE/g) and flavanol (10.44 mg CE/g) contents. Methanol extract of leaves and water extract of stem bark showed high flavonoids (20.94 mg RE/g) and phenolic acid (90.40 mg CAE/g) content, respectively. In addition, HPLC-ESI-TOFMS analysis revealed that U. togoensis was rich in procyanidins. The methanol and water extracts of stem bark had overall superior antioxidant activity; however, only methanol extract of stem bark showed higher inhibition of cholinesterase (AChE: 2.57 mg GALAE/g; BChE: 4.69 mg GALAE/g), tyrosinase (69.53 mg KAE/g) and elastase (2.73 mmol CE/g). Potent metal chelating ability was showed by water extract of leaves (18.94 mg EDTAE/g), higher inhibition of amylase was detected for water extracts of leaves (0.94 mmol ACAE/g) and stem bark (0.92 mmol ACAE/g). The tested extracts have shown wide-spectrum antibacterial properties and these effects have shown to be more effective against Aspergillus ochraceus, Penicillium funiculosum, Trichoderma viride, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The results revealed that the antioxidant, enzyme inhibitory and antimicrobial activities depended on the extraction solvents and the parts of plant. Bioinformatics analysis on the 17 major compounds showed modulation of pathway associated with cancer. In brief, U. togoensis might be valuable as potential source of natural agents for therapeutic application.",
publisher = "Zurich, Switzerland: Wiley-VHCA AG",
journal = "Chemistry &  Biodiversity",
title = "A Prospective of Multiple Biopharmaceutical Activities of Procyanidins-Rich Uapaca togoensis Pax Extracts: HPLC-ESI-TOF-MS Coupled with Bioinformatics Analysis",
number = "8",
volume = "18",
doi = "10.1002/cbdv.202100299",
pages = "e2100299"
}

Sinan, K. I., Aktumsek, A., Cádiz-Gurrea, M. d. l. L., Leyva-Jiménez, F. J., Fernández-Ochoa, Á., Segura-Carretero, A., Glamočlija, J., Soković, M., Nenadic, M.,& Zengin, G.. (2021). A Prospective of Multiple Biopharmaceutical Activities of Procyanidins-Rich Uapaca togoensis Pax Extracts: HPLC-ESI-TOF-MS Coupled with Bioinformatics Analysis. in Chemistry &  Biodiversity
Zurich, Switzerland: Wiley-VHCA AG., 18(8), e2100299.
https://doi.org/10.1002/cbdv.202100299

Sinan KI, Aktumsek A, Cádiz-Gurrea MDLL, Leyva-Jiménez FJ, Fernández-Ochoa Á, Segura-Carretero A, Glamočlija J, Soković M, Nenadic M, Zengin G. A Prospective of Multiple Biopharmaceutical Activities of Procyanidins-Rich Uapaca togoensis Pax Extracts: HPLC-ESI-TOF-MS Coupled with Bioinformatics Analysis. in Chemistry &  Biodiversity. 2021;18(8):e2100299.
doi:10.1002/cbdv.202100299 .

Sinan, Kouadio Ibrahime, Aktumsek, Abdurrahman, Cádiz-Gurrea, María de la Luz, Leyva-Jiménez, Francisco Javier, Fernández-Ochoa, Álvaro, Segura-Carretero, Antonio, Glamočlija, Jasmina, Soković, Marina, Nenadic, Marija, Zengin, Gokhan, "A Prospective of Multiple Biopharmaceutical Activities of Procyanidins-Rich Uapaca togoensis Pax Extracts: HPLC-ESI-TOF-MS Coupled with Bioinformatics Analysis" in Chemistry &  Biodiversity, 18, no. 8 (2021):e2100299,
https://doi.org/10.1002/cbdv.202100299 . .

TY  - JOUR
AU  - Sinan, Kouadio Ibrahime
AU  - Dall’Acqua, Stefano
AU  - Ferrarese, Irene
AU  - Mollica, Adriano
AU  - Stefanucci, Azzurra
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Nenadić, Marija
AU  - Aktumsek, Abdurrahman
AU  - Zengin, Gokhan
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/10/1570
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4500
AB  - The impact of two extraction solvents on the phenolic composition, antioxidant, and enzymes inhibitory and antimicrobial activities of two parts (leaves and stem bark) of P. kotschyi was studied. Two different LC-DAD-MSn approaches were used to identify and quantify the bioactive compounds in the different extracts. A total of thirty-two compounds were quantified, being the procyanidin the most abundant in stem bark while catechin and flavonoids are most abundant in leaves. Overall, the stem bark extraction using methanol showed higher amounts of total phenolic (131.83 ± 1.81 mg GAE/g) and flavanol (14.14 ± 0.11 mg CE/g) while the leaves extraction using water exhibited stronger levels of total flavonoid (44.95 ± 0.38 mg RE/g) and phenolic acid (63.58 ± 2.00 mg CAE/g). As regards the antioxidant assays, methanol stem bark extracts were characterized by the highest antioxidant activities (DPPH: 1.94 ± 0.01 mmol TE/g, ABTS: 3.31 ± 0.01 mmol TE/g, FRAP: 2.86 ± 0.02 mmol TE/g, CUPRAC: 5.09 ± 0.08 mmol TE/g, phosphomolybdenum: 5.16 ± 0.23 mmol TE/g and metal chelating: 17.12 ± 0.46 mg EDTAE/g). In addition, the methanolic extracts of stem bark had highest impact on acetylcholinesterase (2.54 mg GALAE/g), butyrylcholinesterase (5.48 mg GALAE/g). In contrast, the methanolic extracts of leaves was potent against tyrosinase (77.39 ± 0.21 mg KAE/g) and α-glucosidase (0.97 ± 0.01 mmol ACAE/g), while a higher anti-α–amylase (0.97 ± 0.01 mmol ACAE/g) was observed for water extracts of the same part. All of the tested extracts showed inhibitory effects on elastase, except methanolic leaves extracts. Additionally, the extracts exhibited appreciable antifungal toward A. ochraceus, A. fumigatus, P. ochrochloron, T. viride, and P. funiculosum and promising antibacterial activity against M. flavus, S. aureus, L. monocytogenes, E. coli, P. aeruginosa, E. cloacae, and S. typhimurium. Taken together, the outcomes demonstrated P. kotschyi as a novel source of bioactive molecules of interest with an evident therapeutic value.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds
IS  - 10
VL  - 10
DO  - 10.3390/antiox10101570
SP  - 1570
ER  - 

@article{
author = "Sinan, Kouadio Ibrahime and Dall’Acqua, Stefano and Ferrarese, Irene and Mollica, Adriano and Stefanucci, Azzurra and Glamočlija, Jasmina and Soković, Marina and Nenadić, Marija and Aktumsek, Abdurrahman and Zengin, Gokhan",
year = "2021",
abstract = "The impact of two extraction solvents on the phenolic composition, antioxidant, and enzymes inhibitory and antimicrobial activities of two parts (leaves and stem bark) of P. kotschyi was studied. Two different LC-DAD-MSn approaches were used to identify and quantify the bioactive compounds in the different extracts. A total of thirty-two compounds were quantified, being the procyanidin the most abundant in stem bark while catechin and flavonoids are most abundant in leaves. Overall, the stem bark extraction using methanol showed higher amounts of total phenolic (131.83 ± 1.81 mg GAE/g) and flavanol (14.14 ± 0.11 mg CE/g) while the leaves extraction using water exhibited stronger levels of total flavonoid (44.95 ± 0.38 mg RE/g) and phenolic acid (63.58 ± 2.00 mg CAE/g). As regards the antioxidant assays, methanol stem bark extracts were characterized by the highest antioxidant activities (DPPH: 1.94 ± 0.01 mmol TE/g, ABTS: 3.31 ± 0.01 mmol TE/g, FRAP: 2.86 ± 0.02 mmol TE/g, CUPRAC: 5.09 ± 0.08 mmol TE/g, phosphomolybdenum: 5.16 ± 0.23 mmol TE/g and metal chelating: 17.12 ± 0.46 mg EDTAE/g). In addition, the methanolic extracts of stem bark had highest impact on acetylcholinesterase (2.54 mg GALAE/g), butyrylcholinesterase (5.48 mg GALAE/g). In contrast, the methanolic extracts of leaves was potent against tyrosinase (77.39 ± 0.21 mg KAE/g) and α-glucosidase (0.97 ± 0.01 mmol ACAE/g), while a higher anti-α–amylase (0.97 ± 0.01 mmol ACAE/g) was observed for water extracts of the same part. All of the tested extracts showed inhibitory effects on elastase, except methanolic leaves extracts. Additionally, the extracts exhibited appreciable antifungal toward A. ochraceus, A. fumigatus, P. ochrochloron, T. viride, and P. funiculosum and promising antibacterial activity against M. flavus, S. aureus, L. monocytogenes, E. coli, P. aeruginosa, E. cloacae, and S. typhimurium. Taken together, the outcomes demonstrated P. kotschyi as a novel source of bioactive molecules of interest with an evident therapeutic value.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds",
number = "10",
volume = "10",
doi = "10.3390/antiox10101570",
pages = "1570"
}

Sinan, K. I., Dall’Acqua, S., Ferrarese, I., Mollica, A., Stefanucci, A., Glamočlija, J., Soković, M., Nenadić, M., Aktumsek, A.,& Zengin, G.. (2021). LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds. in Antioxidants
Basel: MDPI., 10(10), 1570.
https://doi.org/10.3390/antiox10101570

Sinan KI, Dall’Acqua S, Ferrarese I, Mollica A, Stefanucci A, Glamočlija J, Soković M, Nenadić M, Aktumsek A, Zengin G. LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds. in Antioxidants. 2021;10(10):1570.
doi:10.3390/antiox10101570 .

Sinan, Kouadio Ibrahime, Dall’Acqua, Stefano, Ferrarese, Irene, Mollica, Adriano, Stefanucci, Azzurra, Glamočlija, Jasmina, Soković, Marina, Nenadić, Marija, Aktumsek, Abdurrahman, Zengin, Gokhan, "LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds" in Antioxidants, 10, no. 10 (2021):1570,
https://doi.org/10.3390/antiox10101570 . .

TY  - JOUR
AU  - Sinan, Kouadio Ibrahime
AU  - Luz Cádiz-Gurrea, María de la
AU  - Javier Leyva-Jiménez, Francisco
AU  - Fernández-Ochoa, Álvaro
AU  - Segura-Carretero, Antonio
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Nenadić, Marija
AU  - Aktumsek, Abdurrahman
AU  - Dall'Acqua, Stefano
AU  - Zengin, Gokhan
PY  - 2021
UR  - http://xlink.rsc.org/?DOI=D1NJ03621A
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4685
AB  - In this paper, the methanol and water extracts of Phyllanthus reticulatus Poir. leaves and stem bark parts were considered as the source of bioactive constituents; thus, chemical analysis and bioactivity assays were performed. UPLC-ESI-QTOF/MS analysis allowed the identification of phytoconstituents in the various extracts, including 19 phenolic acids, 27 flavonoids, 20 tannins and 4 iridoids. The methanol extract of stem bark presented a higher antioxidant activity (DPPH: 3.04 mmol TE g(-1); ABTS: 4.72 mmol TE g(-1); CUPRAC: 3.61 mmol TE g(-1) and FRAP: 1.64 mmol TE g(-1)) compared with other extracts. The highest inhibitory effects (AChE: 2.56 mg GALAE g(-1); tyrosinase: 76.25 mg KAE g(-1); amylase: 0.71 mmol ACAE g(-1); glucosidase: 0.98 mmol ACAE g(-1) and elastase: 2.64 mg CE g(-1)) were also recorded by the methanol extract of stem bark. The bacteriostatic (MIC) and bactericidal (MBC) effects of the extracts obtained from different plant parts and using different solvents were evaluated using clinically relevant microorganisms and the measured values were in the range of the activity measured for the reference compounds streptomycin and ampicillin. Bioinformatics analysis was performed to predict the potential toxicity of the identified compounds as well as potential targets of bioactivity. Five of the major compounds, erythrin, gardenoside, phlorizin, sesamoside and veracylglucan B, were identified as possible modulators of necroptosis pathways. Besides, particular attention must be paid to some identified compounds (i.e. icariin, isomyricitrin, methylmyricetin rhamnoside-gallate, rhapontin, silybin, xyloccensin R, vanilloylajugol) because of their probable immunotoxicity predicted properties. Our results could provide a great scientific starting point on the way from the bench to functional applications through the use of P. reticulatus, namely novel nutraceuticals, pharmaceuticals and cosmeceuticals.
PB  - Cambridge: Royal Society of Chemistry
T2  - New Journal of Chemistry
T1  - New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis
IS  - 45
VL  - 45
DO  - 10.1039/D1NJ03621A
SP  - 21049
EP  - 21065
ER  - 

@article{
author = "Sinan, Kouadio Ibrahime and Luz Cádiz-Gurrea, María de la and Javier Leyva-Jiménez, Francisco and Fernández-Ochoa, Álvaro and Segura-Carretero, Antonio and Glamočlija, Jasmina and Soković, Marina and Nenadić, Marija and Aktumsek, Abdurrahman and Dall'Acqua, Stefano and Zengin, Gokhan",
year = "2021",
abstract = "In this paper, the methanol and water extracts of Phyllanthus reticulatus Poir. leaves and stem bark parts were considered as the source of bioactive constituents; thus, chemical analysis and bioactivity assays were performed. UPLC-ESI-QTOF/MS analysis allowed the identification of phytoconstituents in the various extracts, including 19 phenolic acids, 27 flavonoids, 20 tannins and 4 iridoids. The methanol extract of stem bark presented a higher antioxidant activity (DPPH: 3.04 mmol TE g(-1); ABTS: 4.72 mmol TE g(-1); CUPRAC: 3.61 mmol TE g(-1) and FRAP: 1.64 mmol TE g(-1)) compared with other extracts. The highest inhibitory effects (AChE: 2.56 mg GALAE g(-1); tyrosinase: 76.25 mg KAE g(-1); amylase: 0.71 mmol ACAE g(-1); glucosidase: 0.98 mmol ACAE g(-1) and elastase: 2.64 mg CE g(-1)) were also recorded by the methanol extract of stem bark. The bacteriostatic (MIC) and bactericidal (MBC) effects of the extracts obtained from different plant parts and using different solvents were evaluated using clinically relevant microorganisms and the measured values were in the range of the activity measured for the reference compounds streptomycin and ampicillin. Bioinformatics analysis was performed to predict the potential toxicity of the identified compounds as well as potential targets of bioactivity. Five of the major compounds, erythrin, gardenoside, phlorizin, sesamoside and veracylglucan B, were identified as possible modulators of necroptosis pathways. Besides, particular attention must be paid to some identified compounds (i.e. icariin, isomyricitrin, methylmyricetin rhamnoside-gallate, rhapontin, silybin, xyloccensin R, vanilloylajugol) because of their probable immunotoxicity predicted properties. Our results could provide a great scientific starting point on the way from the bench to functional applications through the use of P. reticulatus, namely novel nutraceuticals, pharmaceuticals and cosmeceuticals.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "New Journal of Chemistry",
title = "New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis",
number = "45",
volume = "45",
doi = "10.1039/D1NJ03621A",
pages = "21049-21065"
}

Sinan, K. I., Luz Cádiz-Gurrea, M. d. l., Javier Leyva-Jiménez, F., Fernández-Ochoa, Á., Segura-Carretero, A., Glamočlija, J., Soković, M., Nenadić, M., Aktumsek, A., Dall'Acqua, S.,& Zengin, G.. (2021). New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis. in New Journal of Chemistry
Cambridge: Royal Society of Chemistry., 45(45), 21049-21065.
https://doi.org/10.1039/D1NJ03621A

Sinan KI, Luz Cádiz-Gurrea MDL, Javier Leyva-Jiménez F, Fernández-Ochoa Á, Segura-Carretero A, Glamočlija J, Soković M, Nenadić M, Aktumsek A, Dall'Acqua S, Zengin G. New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis. in New Journal of Chemistry. 2021;45(45):21049-21065.
doi:10.1039/D1NJ03621A .

Sinan, Kouadio Ibrahime, Luz Cádiz-Gurrea, María de la, Javier Leyva-Jiménez, Francisco, Fernández-Ochoa, Álvaro, Segura-Carretero, Antonio, Glamočlija, Jasmina, Soković, Marina, Nenadić, Marija, Aktumsek, Abdurrahman, Dall'Acqua, Stefano, Zengin, Gokhan, "New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis" in New Journal of Chemistry, 45, no. 45 (2021):21049-21065,
https://doi.org/10.1039/D1NJ03621A . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Cvetanović, Aleksandra
AU  - Gašić, Uroš
AU  - Stupar, Alena
AU  - Bulut, Gizem
AU  - Senkardes, Ismail
AU  - Dogan, Ahmet
AU  - Sinan, Kouadio Ibrahime
AU  - Uysal, Sengul
AU  - Aumeeruddy-Elalfi, Zaahiri
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2020
UR  - https://doi.org/10.1016/j.indcrop.2020.112202
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3609
AB  - Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.
T2  - Industrial Crops & Products
T1  - Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip
IS  - April 2020
VL  - 146
DO  - 10.1016/j.indcrop.2020.112202
SP  - 112202
ER  - 

@article{
author = "Zengin, Gokhan and Cvetanović, Aleksandra and Gašić, Uroš and Stupar, Alena and Bulut, Gizem and Senkardes, Ismail and Dogan, Ahmet and Sinan, Kouadio Ibrahime and Uysal, Sengul and Aumeeruddy-Elalfi, Zaahiri and Aktumsek, Abdurrahman and Mahomoodally, Mohamad Fawzi",
year = "2020",
abstract = "Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.",
journal = "Industrial Crops & Products",
title = "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip",
number = "April 2020",
volume = "146",
doi = "10.1016/j.indcrop.2020.112202",
pages = "112202"
}

Zengin, G., Cvetanović, A., Gašić, U., Stupar, A., Bulut, G., Senkardes, I., Dogan, A., Sinan, K. I., Uysal, S., Aumeeruddy-Elalfi, Z., Aktumsek, A.,& Mahomoodally, M. F.. (2020). Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products, 146(April 2020), 112202.
https://doi.org/10.1016/j.indcrop.2020.112202

Zengin G, Cvetanović A, Gašić U, Stupar A, Bulut G, Senkardes I, Dogan A, Sinan KI, Uysal S, Aumeeruddy-Elalfi Z, Aktumsek A, Mahomoodally MF. Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products. 2020;146(April 2020):112202.
doi:10.1016/j.indcrop.2020.112202 .

Zengin, Gokhan, Cvetanović, Aleksandra, Gašić, Uroš, Stupar, Alena, Bulut, Gizem, Senkardes, Ismail, Dogan, Ahmet, Sinan, Kouadio Ibrahime, Uysal, Sengul, Aumeeruddy-Elalfi, Zaahiri, Aktumsek, Abdurrahman, Mahomoodally, Mohamad Fawzi, "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip" in Industrial Crops & Products, 146, no. April 2020 (2020):112202,
https://doi.org/10.1016/j.indcrop.2020.112202 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Zheleva-Dimitrova, Dimitrina
AU  - Gevrenova, Reneta
AU  - Nedialkov, Paraskev
AU  - Mocan, Andrei
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, M.Fawzi
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0731708517325074
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2931
AB  - The Centaurea genus has great potential in traditional systems and has attracted much interest in the design of novel drug formulations. The present study was focused on the chemical fingerprints and biological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spectrophotometric and LC-MS techniques were used to establish the chemical profiles of the studied extracts. Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namely neurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes (α-amylase and α-glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterial and fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPH and ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metal chelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid content of the extracts were in the range of 2.88-22.39 mgRE/g extract. Methanol and water extracts showed stronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts were most efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted considerable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifolia subsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open new avenues for research and contribute towards establishing primary data on these species for designing novel phytopharmaceuticals.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia
VL  - 149
DO  - 10.1016/j.jpba.2017.11.045
SP  - 436
EP  - 441
ER  - 

@article{
author = "Zengin, Gokhan and Zheleva-Dimitrova, Dimitrina and Gevrenova, Reneta and Nedialkov, Paraskev and Mocan, Andrei and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Aktumsek, Abdurrahman and Mahomoodally, M.Fawzi",
year = "2018",
abstract = "The Centaurea genus has great potential in traditional systems and has attracted much interest in the design of novel drug formulations. The present study was focused on the chemical fingerprints and biological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spectrophotometric and LC-MS techniques were used to establish the chemical profiles of the studied extracts. Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namely neurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes (α-amylase and α-glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterial and fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPH and ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metal chelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid content of the extracts were in the range of 2.88-22.39 mgRE/g extract. Methanol and water extracts showed stronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts were most efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted considerable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifolia subsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open new avenues for research and contribute towards establishing primary data on these species for designing novel phytopharmaceuticals.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia",
volume = "149",
doi = "10.1016/j.jpba.2017.11.045",
pages = "436-441"
}

Zengin, G., Zheleva-Dimitrova, D., Gevrenova, R., Nedialkov, P., Mocan, A., Ćirić, A., Glamočlija, J., Soković, M., Aktumsek, A.,& Mahomoodally, M.Fawzi. (2018). Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis, 149, 436-441.
https://doi.org/10.1016/j.jpba.2017.11.045

Zengin G, Zheleva-Dimitrova D, Gevrenova R, Nedialkov P, Mocan A, Ćirić A, Glamočlija J, Soković M, Aktumsek A, Mahomoodally M. Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis. 2018;149:436-441.
doi:10.1016/j.jpba.2017.11.045 .

Zengin, Gokhan, Zheleva-Dimitrova, Dimitrina, Gevrenova, Reneta, Nedialkov, Paraskev, Mocan, Andrei, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Aktumsek, Abdurrahman, Mahomoodally, M.Fawzi, "Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia" in Journal of Pharmaceutical and Biomedical Analysis, 149 (2018):436-441,
https://doi.org/10.1016/j.jpba.2017.11.045 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Mahomoodally, M. Fawzi
AU  - Aktumsek, Abdurrahman
AU  - Ceylan, Ramazan
AU  - Uysal, Sengul
AU  - Mocan, Andrei
AU  - Yilmaz, Mustafa Abdullah
AU  - Picot-Allain, Carene Marie Nancy
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S2212429217305473?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3090
AB  - Six wild species (S. alba, S. conoidea, S. dichotoma, S. italica, S. supina, and S. vulgaris) from the Silene genera were tested for potential anti-enzymatic (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase), antimicrobial (16 microbial strains), and antioxidant activity. An ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method was used for phytochemical determination. Quinic acid, malic acid, protocatechuic acid, p-coumaric acid, and hesperidin were common in the six Silene species. All extracts showed higher antibacterial effects compared to streptomycin and ampicillin (except S. dichotoma). Antifungal agents, bifonazole (MIC 0.10–0.20 mg/mL and MFC 0.20–0.30 mg/mL) and ketoconazole (MIC 0.15–2.30 mg/mL and MFC 0.20–3.50 mg/mL) showed lower activity than the investigated Silene species extracts. S. alba inhibited AChE (2.00 mg GALAE/g extract) and BChE (1.0 mg GALAE/g extract). The results showed metal chelating potential ranging from 12 to 19 mg EDTAE/g extract, with S. conoidea being the most active, and S. supina the least. S. dichotoma showed the highest reducing potential against both cupric (154 mg TE/g extract for CUPRAC) and ferric (102 mg TE/g extract for FRAP) ions. Overall, Silene species could be considered as emerging interesting functional foods and sources of nutraceuticals with applications in the management of different diseases.
T2  - Food Bioscience
T1  - Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties
VL  - 23
DO  - 10.1016/J.FBIO.2018.03.010
SP  - 75
EP  - 82
ER  - 

@article{
author = "Zengin, Gokhan and Mahomoodally, M. Fawzi and Aktumsek, Abdurrahman and Ceylan, Ramazan and Uysal, Sengul and Mocan, Andrei and Yilmaz, Mustafa Abdullah and Picot-Allain, Carene Marie Nancy and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2018",
abstract = "Six wild species (S. alba, S. conoidea, S. dichotoma, S. italica, S. supina, and S. vulgaris) from the Silene genera were tested for potential anti-enzymatic (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase), antimicrobial (16 microbial strains), and antioxidant activity. An ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method was used for phytochemical determination. Quinic acid, malic acid, protocatechuic acid, p-coumaric acid, and hesperidin were common in the six Silene species. All extracts showed higher antibacterial effects compared to streptomycin and ampicillin (except S. dichotoma). Antifungal agents, bifonazole (MIC 0.10–0.20 mg/mL and MFC 0.20–0.30 mg/mL) and ketoconazole (MIC 0.15–2.30 mg/mL and MFC 0.20–3.50 mg/mL) showed lower activity than the investigated Silene species extracts. S. alba inhibited AChE (2.00 mg GALAE/g extract) and BChE (1.0 mg GALAE/g extract). The results showed metal chelating potential ranging from 12 to 19 mg EDTAE/g extract, with S. conoidea being the most active, and S. supina the least. S. dichotoma showed the highest reducing potential against both cupric (154 mg TE/g extract for CUPRAC) and ferric (102 mg TE/g extract for FRAP) ions. Overall, Silene species could be considered as emerging interesting functional foods and sources of nutraceuticals with applications in the management of different diseases.",
journal = "Food Bioscience",
title = "Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties",
volume = "23",
doi = "10.1016/J.FBIO.2018.03.010",
pages = "75-82"
}

Zengin, G., Mahomoodally, M. F., Aktumsek, A., Ceylan, R., Uysal, S., Mocan, A., Yilmaz, M. A., Picot-Allain, C. M. N., Ćirić, A., Glamočlija, J.,& Soković, M.. (2018). Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties. in Food Bioscience, 23, 75-82.
https://doi.org/10.1016/J.FBIO.2018.03.010

Zengin G, Mahomoodally MF, Aktumsek A, Ceylan R, Uysal S, Mocan A, Yilmaz MA, Picot-Allain CMN, Ćirić A, Glamočlija J, Soković M. Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties. in Food Bioscience. 2018;23:75-82.
doi:10.1016/J.FBIO.2018.03.010 .

Zengin, Gokhan, Mahomoodally, M. Fawzi, Aktumsek, Abdurrahman, Ceylan, Ramazan, Uysal, Sengul, Mocan, Andrei, Yilmaz, Mustafa Abdullah, Picot-Allain, Carene Marie Nancy, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties" in Food Bioscience, 23 (2018):75-82,
https://doi.org/10.1016/J.FBIO.2018.03.010 . .

TY  - JOUR
AU  - Uysal, Sengul
AU  - Aumeeruddy-Elalfi, Zaahira
AU  - Zengin, Gokhan
AU  - Aktumsek, Abdurrahman
AU  - Mocan, Andrei
AU  - Custodio, Luisa
AU  - Neng, Nuno R.
AU  - Nogueira, José M.F.
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Mahomoodally, M.Fawzi
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0926669018300013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2965
AB  - Ballota macrodonta Boiss. et Balansa., is a traditionally used endemic medicinal plant used to manage a plethora of diseases in Turkey. Nonetheless, few studies have endeavoured to highlight its therapeutic potential and its phytochemical profile. We hypothesized that B. macrodonta would possess multi-pharmacological propensities and bioactive compounds that would justify its use as a folk remedy. Aerial part of B. macrodonta was assessed for its antioxidant, antimicrobial, and enzymatic inhibitory potential using different extraction solvents. The total bioactive components determination and compound identification using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) method were used to correlate the biological properties. HPLC-DAD profile showed the presence of 19 components (e.g. hydroxybenzoic acid, catechin hydrate, vanillic acid, caffeic acid, syringic acid, epicatechin, coumaric acid, ferulic acid, luteolin-7-O-glucoside, rutin, and quercetin). The water and methanolic extracts showed high total phenolic and flavonoid contents, respectively. The overall antioxidant capacity of the water extract was higher than the one of the methanol extract. The extracts showed inhibitory activity against cholinesterases, tyrosinase, α-amylase and α-glucosidase. The extracts also presented antimicrobial activity against eight Gram positive/negative bacteria (MIC – 0.05–0.15 mg/mL) and eight fungi (MIC – 0.0125–0.20 mg/mL). The presence of active biomolecules with multi-pharmacological properties makes B. macrodonta a potential source of health-promoting compounds that can be exploited as a novel medicinal herbal product.
T2  - Industrial Crops and Products
T1  - Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey
VL  - 113
DO  - 10.1016/j.indcrop.2018.01.001
SP  - 422
EP  - 428
ER  - 

@article{
author = "Uysal, Sengul and Aumeeruddy-Elalfi, Zaahira and Zengin, Gokhan and Aktumsek, Abdurrahman and Mocan, Andrei and Custodio, Luisa and Neng, Nuno R. and Nogueira, José M.F. and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Mahomoodally, M.Fawzi",
year = "2018",
abstract = "Ballota macrodonta Boiss. et Balansa., is a traditionally used endemic medicinal plant used to manage a plethora of diseases in Turkey. Nonetheless, few studies have endeavoured to highlight its therapeutic potential and its phytochemical profile. We hypothesized that B. macrodonta would possess multi-pharmacological propensities and bioactive compounds that would justify its use as a folk remedy. Aerial part of B. macrodonta was assessed for its antioxidant, antimicrobial, and enzymatic inhibitory potential using different extraction solvents. The total bioactive components determination and compound identification using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) method were used to correlate the biological properties. HPLC-DAD profile showed the presence of 19 components (e.g. hydroxybenzoic acid, catechin hydrate, vanillic acid, caffeic acid, syringic acid, epicatechin, coumaric acid, ferulic acid, luteolin-7-O-glucoside, rutin, and quercetin). The water and methanolic extracts showed high total phenolic and flavonoid contents, respectively. The overall antioxidant capacity of the water extract was higher than the one of the methanol extract. The extracts showed inhibitory activity against cholinesterases, tyrosinase, α-amylase and α-glucosidase. The extracts also presented antimicrobial activity against eight Gram positive/negative bacteria (MIC – 0.05–0.15 mg/mL) and eight fungi (MIC – 0.0125–0.20 mg/mL). The presence of active biomolecules with multi-pharmacological properties makes B. macrodonta a potential source of health-promoting compounds that can be exploited as a novel medicinal herbal product.",
journal = "Industrial Crops and Products",
title = "Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey",
volume = "113",
doi = "10.1016/j.indcrop.2018.01.001",
pages = "422-428"
}

Uysal, S., Aumeeruddy-Elalfi, Z., Zengin, G., Aktumsek, A., Mocan, A., Custodio, L., Neng, N. R., Nogueira, J. M.F., Ćirić, A., Glamočlija, J., Soković, M.,& Mahomoodally, M.Fawzi. (2018). Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey. in Industrial Crops and Products, 113, 422-428.
https://doi.org/10.1016/j.indcrop.2018.01.001

Uysal S, Aumeeruddy-Elalfi Z, Zengin G, Aktumsek A, Mocan A, Custodio L, Neng NR, Nogueira JM, Ćirić A, Glamočlija J, Soković M, Mahomoodally M. Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey. in Industrial Crops and Products. 2018;113:422-428.
doi:10.1016/j.indcrop.2018.01.001 .

Uysal, Sengul, Aumeeruddy-Elalfi, Zaahira, Zengin, Gokhan, Aktumsek, Abdurrahman, Mocan, Andrei, Custodio, Luisa, Neng, Nuno R., Nogueira, José M.F., Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Mahomoodally, M.Fawzi, "Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey" in Industrial Crops and Products, 113 (2018):422-428,
https://doi.org/10.1016/j.indcrop.2018.01.001 . .

TY  - JOUR
AU  - Zengin, Gökhan
AU  - Zheleva-Dimitrova, Dimitrina
AU  - Gevrenova, Reneta
AU  - Nedialkov, Paraskev
AU  - Mocan, Andrei
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, Muhammed Fettah
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4304
AB  - The Centaurea genus has great potential in traditional systems and has attracted much interest in thedesign of novel drug formulations. The present study was focused on the chemical fingerprints andbiological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spec-trophotometric and LC–MS techniques were used to establish the chemical profiles of the studied extracts.Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namelyneurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes ( -amylaseand  -glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterialand fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPHand ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metalchelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid con-tent of the extracts were in the range of 2.88–22.39 mgRE/g extract. Methanol and water extracts showedstronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts weremost efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted con-siderable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifoliasubsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open newavenues for research and contribute towards establishing primary data on these species for designingnovel phytopharmaceuticals.
PB  - Elsevier B.V.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia
VL  - 149
DO  - 10.1016/j.jpba.2017.11.045
SP  - 436
EP  - 441
ER  - 

@article{
author = "Zengin, Gökhan and Zheleva-Dimitrova, Dimitrina and Gevrenova, Reneta and Nedialkov, Paraskev and Mocan, Andrei and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Aktumsek, Abdurrahman and Mahomoodally, Muhammed Fettah",
year = "2018",
abstract = "The Centaurea genus has great potential in traditional systems and has attracted much interest in thedesign of novel drug formulations. The present study was focused on the chemical fingerprints andbiological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spec-trophotometric and LC–MS techniques were used to establish the chemical profiles of the studied extracts.Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namelyneurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes ( -amylaseand  -glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterialand fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPHand ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metalchelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid con-tent of the extracts were in the range of 2.88–22.39 mgRE/g extract. Methanol and water extracts showedstronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts weremost efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted con-siderable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifoliasubsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open newavenues for research and contribute towards establishing primary data on these species for designingnovel phytopharmaceuticals.",
publisher = "Elsevier B.V.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia",
volume = "149",
doi = "10.1016/j.jpba.2017.11.045",
pages = "436-441"
}

Zengin, G., Zheleva-Dimitrova, D., Gevrenova, R., Nedialkov, P., Mocan, A., Ćirić, A., Glamočlija, J., Soković, M., Aktumsek, A.,& Mahomoodally, M. F.. (2018). Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier B.V.., 149, 436-441.
https://doi.org/10.1016/j.jpba.2017.11.045

Zengin G, Zheleva-Dimitrova D, Gevrenova R, Nedialkov P, Mocan A, Ćirić A, Glamočlija J, Soković M, Aktumsek A, Mahomoodally MF. Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis. 2018;149:436-441.
doi:10.1016/j.jpba.2017.11.045 .

Zengin, Gökhan, Zheleva-Dimitrova, Dimitrina, Gevrenova, Reneta, Nedialkov, Paraskev, Mocan, Andrei, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Aktumsek, Abdurrahman, Mahomoodally, Muhammed Fettah, "Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia" in Journal of Pharmaceutical and Biomedical Analysis, 149 (2018):436-441,
https://doi.org/10.1016/j.jpba.2017.11.045 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Aktumsek, Abdurrahman
AU  - Ceylan, Ramazan
AU  - Uysal, Sengul
AU  - Mocan, Andrei
AU  - Guler, Gokalp Ozmen
AU  - Mahomoodally, M. Fawzi
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2017
UR  - http://xlink.rsc.org/?DOI=C6FO01847E
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2728
AB  - Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the biological and chemical profiles of different extracts (ethyl acetate, methanol and water) obtained from three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). The antioxidant (free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum), enzyme inhibitory (anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase) and antimicrobial (antibacterial and antifungal) effects were assessed to investigate their biological profiles. Moreover, the total phenolic and flavonoid contents were determined and LC-MS analysis was performed for the chemical profile of the investigated extracts. The LC-MS analysis revealed the presence of several caffeoylquinic acids in these extracts. Generally, the methanol and water extracts exhibited stronger antioxidant abilities, which correlated with the higher levels of phenolic compounds when compared to the ethyl acetate extracts. In addition, the best antimicrobial activities were obtained for the ethyl acetate and methanolic extracts. However, the ethyl acetate extract had remarkable enzyme inhibitory potential. On the basis of our results, Achillea species may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients.
T2  - Food and Function
T1  - Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia)
IS  - 3
VL  - 8
DO  - 10.1039/C6FO01847E
SP  - 1152
EP  - 1165
ER  - 

@article{
author = "Zengin, Gokhan and Aktumsek, Abdurrahman and Ceylan, Ramazan and Uysal, Sengul and Mocan, Andrei and Guler, Gokalp Ozmen and Mahomoodally, M. Fawzi and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2017",
abstract = "Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the biological and chemical profiles of different extracts (ethyl acetate, methanol and water) obtained from three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). The antioxidant (free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum), enzyme inhibitory (anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase) and antimicrobial (antibacterial and antifungal) effects were assessed to investigate their biological profiles. Moreover, the total phenolic and flavonoid contents were determined and LC-MS analysis was performed for the chemical profile of the investigated extracts. The LC-MS analysis revealed the presence of several caffeoylquinic acids in these extracts. Generally, the methanol and water extracts exhibited stronger antioxidant abilities, which correlated with the higher levels of phenolic compounds when compared to the ethyl acetate extracts. In addition, the best antimicrobial activities were obtained for the ethyl acetate and methanolic extracts. However, the ethyl acetate extract had remarkable enzyme inhibitory potential. On the basis of our results, Achillea species may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients.",
journal = "Food and Function",
title = "Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia)",
number = "3",
volume = "8",
doi = "10.1039/C6FO01847E",
pages = "1152-1165"
}

Zengin, G., Aktumsek, A., Ceylan, R., Uysal, S., Mocan, A., Guler, G. O., Mahomoodally, M. F., Glamočlija, J., Ćirić, A.,& Soković, M.. (2017). Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). in Food and Function, 8(3), 1152-1165.
https://doi.org/10.1039/C6FO01847E

Zengin G, Aktumsek A, Ceylan R, Uysal S, Mocan A, Guler GO, Mahomoodally MF, Glamočlija J, Ćirić A, Soković M. Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). in Food and Function. 2017;8(3):1152-1165.
doi:10.1039/C6FO01847E .

Zengin, Gokhan, Aktumsek, Abdurrahman, Ceylan, Ramazan, Uysal, Sengul, Mocan, Andrei, Guler, Gokalp Ozmen, Mahomoodally, M. Fawzi, Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia)" in Food and Function, 8, no. 3 (2017):1152-1165,
https://doi.org/10.1039/C6FO01847E . .

TY  - JOUR
AU  - Savran, Ahmet
AU  - Zengin, Gokhan
AU  - Aktumsek, Abdurrahman
AU  - Mocan, Andrei
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6746
AB  - The present study outlines a chemical characterization and further effects beneficial to health of edible
Rumex scutatus and Pseudosempervivum sempervivum, in addition to presenting the antioxidant,
enzyme inhibitory effects and antimicrobial properties of different extracts. The phenolic compounds
composition of the extracts was assessed by RP-HPLC-DAD, outlining benzoic acid and rutin as major
constituents in P. sempervivum and rutin and hesperidin in R. scutatus. Moreover, further biological
effects were tested on key enzymes involved in diabetes mellitus, Alzheimer’s disease and skin melano genesis revealing an important tyrosinase inhibitory effect of Pseudosempervivum water extract. More over, both species possessed antimicrobial properties towards bacteria and fungi relevant to public
health. Accordingly, we find that R. scutatus and P. sempervivum can be considered as novel functional
foods because they are rich sources of biologically active compounds that provide health benefits.
PB  - Royal Society of Chemistry
T2  - Food and Function
T1  - Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits
IS  - 7
VL  - 7
DO  - 10.1039/c6fo00695g
SP  - 3252
EP  - 3262
ER  - 

@article{
author = "Savran, Ahmet and Zengin, Gokhan and Aktumsek, Abdurrahman and Mocan, Andrei and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2016",
abstract = "The present study outlines a chemical characterization and further effects beneficial to health of edible
Rumex scutatus and Pseudosempervivum sempervivum, in addition to presenting the antioxidant,
enzyme inhibitory effects and antimicrobial properties of different extracts. The phenolic compounds
composition of the extracts was assessed by RP-HPLC-DAD, outlining benzoic acid and rutin as major
constituents in P. sempervivum and rutin and hesperidin in R. scutatus. Moreover, further biological
effects were tested on key enzymes involved in diabetes mellitus, Alzheimer’s disease and skin melano genesis revealing an important tyrosinase inhibitory effect of Pseudosempervivum water extract. More over, both species possessed antimicrobial properties towards bacteria and fungi relevant to public
health. Accordingly, we find that R. scutatus and P. sempervivum can be considered as novel functional
foods because they are rich sources of biologically active compounds that provide health benefits.",
publisher = "Royal Society of Chemistry",
journal = "Food and Function",
title = "Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits",
number = "7",
volume = "7",
doi = "10.1039/c6fo00695g",
pages = "3252-3262"
}

Savran, A., Zengin, G., Aktumsek, A., Mocan, A., Glamočlija, J., Ćirić, A.,& Soković, M.. (2016). Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits. in Food and Function
Royal Society of Chemistry., 7(7), 3252-3262.
https://doi.org/10.1039/c6fo00695g

Savran A, Zengin G, Aktumsek A, Mocan A, Glamočlija J, Ćirić A, Soković M. Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits. in Food and Function. 2016;7(7):3252-3262.
doi:10.1039/c6fo00695g .

Savran, Ahmet, Zengin, Gokhan, Aktumsek, Abdurrahman, Mocan, Andrei, Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits" in Food and Function, 7, no. 7 (2016):3252-3262,
https://doi.org/10.1039/c6fo00695g . .