TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5579
AB  - Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)
VL  - 249
DO  - 10.1016/j.aanat.2023.152097
SP  - 152097
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Cvijanović, Milena and Vučić, Tijana and Ivanović, Ana",
year = "2023",
abstract = "Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)",
volume = "249",
doi = "10.1016/j.aanat.2023.152097",
pages = "152097"
}

Ajduković, M., Ukropina, M., Cvijanović, M., Vučić, T.,& Ivanović, A.. (2023). Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH.., 249, 152097.
https://doi.org/10.1016/j.aanat.2023.152097

Ajduković M, Ukropina M, Cvijanović M, Vučić T, Ivanović A. Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger. 2023;249:152097.
doi:10.1016/j.aanat.2023.152097 .

Ajduković, Maja, Ukropina, Mirela, Cvijanović, Milena, Vučić, Tijana, Ivanović, Ana, "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)" in Annals of Anatomy - Anatomischer Anzeiger, 249 (2023):152097,
https://doi.org/10.1016/j.aanat.2023.152097 . .

TY  - JOUR
AU  - Herczeg, Dávid
AU  - Palomar, Gemma
AU  - Zieliński, Piotr
AU  - van Riemsdijk, Isolde
AU  - Babik, Wiesław
AU  - Dankovics, Róbert
AU  - Halpern, Bálint
AU  - Cvijanović, Milena
AU  - Vörös, Judit
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6051
AB  - Species with wide-range distributions usually display high genetic variation. This variation can be partly explained by historical lineages that were temporally isolated from each other and are back into secondary reproductive contact, and partly by local adaptations. The smooth newt (Lissotriton vulgaris) is one of the most widely distributed amphibians species across Eurasia and forms a species complex with a partially overlapping distribution and morphology. In the present study, we explored the population genomic structure of smooth newt lineages in the Carpathian Basin (CB) relying on single-nucleotide polymorphisms. Our dataset included new and previously published data to study the secondary contact zone between lineages in the CB and also tested for the barrier effect of rivers to gene flow between these lineages. We confirmed the presence of the South L. v. vulgaris Lineage distributed in Transdanubia and we provided new distribution records of L. v. ampelensis inhabiting the eastern territories of the CB. High genetic diversity of smooth newts was observed, especially in the North Hungarian Mountains and at the interfluves of the main rivers in the South with four distinct lineages of L. v. vulgaris and one lineage of L. v. ampelensis showing a low level of admixture with the spatially closest L. v. vulgaris lineage. Moreover, admixture detected at the interfluve of the main rivers (i.e. Danube and Tisza) suggested a secondary contact zone in the area. Finally, we found that the river Danube has a very weak effect on population divergence, while the river Tisza is a geographical barrier limiting gene flow between smooth newt lineages. As the range boundaries of L. v. vulgaris and L. v. ampelensis in the CB coincide with the river Tisza, our study underpins the influence of rivers in lineage diversification.
PB  - Hoboken: Wiley
T2  - Ecology and Evolution
T1  - Genomic analysis reveals complex population structure within the smooth newt, Lissotriton vulgaris, in Central Europe
IS  - 9
VL  - 13
DO  - 10.1002/ece3.10478
SP  - e10478
ER  - 

@article{
author = "Herczeg, Dávid and Palomar, Gemma and Zieliński, Piotr and van Riemsdijk, Isolde and Babik, Wiesław and Dankovics, Róbert and Halpern, Bálint and Cvijanović, Milena and Vörös, Judit",
year = "2023",
abstract = "Species with wide-range distributions usually display high genetic variation. This variation can be partly explained by historical lineages that were temporally isolated from each other and are back into secondary reproductive contact, and partly by local adaptations. The smooth newt (Lissotriton vulgaris) is one of the most widely distributed amphibians species across Eurasia and forms a species complex with a partially overlapping distribution and morphology. In the present study, we explored the population genomic structure of smooth newt lineages in the Carpathian Basin (CB) relying on single-nucleotide polymorphisms. Our dataset included new and previously published data to study the secondary contact zone between lineages in the CB and also tested for the barrier effect of rivers to gene flow between these lineages. We confirmed the presence of the South L. v. vulgaris Lineage distributed in Transdanubia and we provided new distribution records of L. v. ampelensis inhabiting the eastern territories of the CB. High genetic diversity of smooth newts was observed, especially in the North Hungarian Mountains and at the interfluves of the main rivers in the South with four distinct lineages of L. v. vulgaris and one lineage of L. v. ampelensis showing a low level of admixture with the spatially closest L. v. vulgaris lineage. Moreover, admixture detected at the interfluve of the main rivers (i.e. Danube and Tisza) suggested a secondary contact zone in the area. Finally, we found that the river Danube has a very weak effect on population divergence, while the river Tisza is a geographical barrier limiting gene flow between smooth newt lineages. As the range boundaries of L. v. vulgaris and L. v. ampelensis in the CB coincide with the river Tisza, our study underpins the influence of rivers in lineage diversification.",
publisher = "Hoboken: Wiley",
journal = "Ecology and Evolution",
title = "Genomic analysis reveals complex population structure within the smooth newt, Lissotriton vulgaris, in Central Europe",
number = "9",
volume = "13",
doi = "10.1002/ece3.10478",
pages = "e10478"
}

Herczeg, D., Palomar, G., Zieliński, P., van Riemsdijk, I., Babik, W., Dankovics, R., Halpern, B., Cvijanović, M.,& Vörös, J.. (2023). Genomic analysis reveals complex population structure within the smooth newt, Lissotriton vulgaris, in Central Europe. in Ecology and Evolution
Hoboken: Wiley., 13(9), e10478.
https://doi.org/10.1002/ece3.10478

Herczeg D, Palomar G, Zieliński P, van Riemsdijk I, Babik W, Dankovics R, Halpern B, Cvijanović M, Vörös J. Genomic analysis reveals complex population structure within the smooth newt, Lissotriton vulgaris, in Central Europe. in Ecology and Evolution. 2023;13(9):e10478.
doi:10.1002/ece3.10478 .

Herczeg, Dávid, Palomar, Gemma, Zieliński, Piotr, van Riemsdijk, Isolde, Babik, Wiesław, Dankovics, Róbert, Halpern, Bálint, Cvijanović, Milena, Vörös, Judit, "Genomic analysis reveals complex population structure within the smooth newt, Lissotriton vulgaris, in Central Europe" in Ecology and Evolution, 13, no. 9 (2023):e10478,
https://doi.org/10.1002/ece3.10478 . .

TY  - JOUR
AU  - Robbemont, Jody
AU  - van Veldhuijzen, Sam
AU  - Allain, Steven J.R.
AU  - Ambu, Johanna
AU  - Boyle, Ryan
AU  - Canestrelli, Daniele
AU  - Cathasaigh, Éinne Ó
AU  - Cathrine, Chris
AU  - Chiocchio, Andrea
AU  - Cogalniceanu, Dan
AU  - Cvijanović, Milena
AU  - Dufresnes, Christophe
AU  - Ennis, Collie
AU  - Gandola, Rob
AU  - Jablonski, Daniel
AU  - Julian, Angela
AU  - Kranželić, Daria
AU  - Lukanov, Simeon
AU  - Martínez-Solano, Iñigo
AU  - Montgomery, Ryan
AU  - Naumov, Borislav
AU  - O’Neill, Matthew
AU  - North, Alexandra
AU  - Pabijan, Maciej
AU  - Pushendorf, Robert
AU  - Salvi, Daniele
AU  - Schmidt, Bruno
AU  - Sotiropoulos, Konstantinos
AU  - Stanescu, Florina
AU  - Stanković, David
AU  - Stapleton, Sarah
AU  - Šunje, Emina
AU  - Szabolcs, Márton
AU  - Vacheva, Emiliya
AU  - Willis, David
AU  - Zimić, Adnan
AU  - France, James
AU  - Meilink, Willem R.M.
AU  - Stark, Tariq
AU  - Struijk, Richard P.J.H.
AU  - Theodoropoulos, Anagnostis
AU  - de Visser, Manon C.
AU  - Wielstra, Ben
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6052
AB  - Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding.
PB  - Brill Academic Publishers
T2  - Amphibia-Reptilia
T1  - An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations
IS  - 3
VL  - 44
DO  - 10.1163/15685381-bja10144
SP  - 347
EP  - 361
ER  - 

@article{
author = "Robbemont, Jody and van Veldhuijzen, Sam and Allain, Steven J.R. and Ambu, Johanna and Boyle, Ryan and Canestrelli, Daniele and Cathasaigh, Éinne Ó and Cathrine, Chris and Chiocchio, Andrea and Cogalniceanu, Dan and Cvijanović, Milena and Dufresnes, Christophe and Ennis, Collie and Gandola, Rob and Jablonski, Daniel and Julian, Angela and Kranželić, Daria and Lukanov, Simeon and Martínez-Solano, Iñigo and Montgomery, Ryan and Naumov, Borislav and O’Neill, Matthew and North, Alexandra and Pabijan, Maciej and Pushendorf, Robert and Salvi, Daniele and Schmidt, Bruno and Sotiropoulos, Konstantinos and Stanescu, Florina and Stanković, David and Stapleton, Sarah and Šunje, Emina and Szabolcs, Márton and Vacheva, Emiliya and Willis, David and Zimić, Adnan and France, James and Meilink, Willem R.M. and Stark, Tariq and Struijk, Richard P.J.H. and Theodoropoulos, Anagnostis and de Visser, Manon C. and Wielstra, Ben",
year = "2023",
abstract = "Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding.",
publisher = "Brill Academic Publishers",
journal = "Amphibia-Reptilia",
title = "An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations",
number = "3",
volume = "44",
doi = "10.1163/15685381-bja10144",
pages = "347-361"
}

Robbemont, J., van Veldhuijzen, S., Allain, S. J.R., Ambu, J., Boyle, R., Canestrelli, D., Cathasaigh, É. Ó., Cathrine, C., Chiocchio, A., Cogalniceanu, D., Cvijanović, M., Dufresnes, C., Ennis, C., Gandola, R., Jablonski, D., Julian, A., Kranželić, D., Lukanov, S., Martínez-Solano, I., Montgomery, R., Naumov, B., O’Neill, M., North, A., Pabijan, M., Pushendorf, R., Salvi, D., Schmidt, B., Sotiropoulos, K., Stanescu, F., Stanković, D., Stapleton, S., Šunje, E., Szabolcs, M., Vacheva, E., Willis, D., Zimić, A., France, J., Meilink, W. R.M., Stark, T., Struijk, R. P.J.H., Theodoropoulos, A., de Visser, M. C.,& Wielstra, B.. (2023). An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations. in Amphibia-Reptilia
Brill Academic Publishers., 44(3), 347-361.
https://doi.org/10.1163/15685381-bja10144

Robbemont J, van Veldhuijzen S, Allain SJ, Ambu J, Boyle R, Canestrelli D, Cathasaigh ÉÓ, Cathrine C, Chiocchio A, Cogalniceanu D, Cvijanović M, Dufresnes C, Ennis C, Gandola R, Jablonski D, Julian A, Kranželić D, Lukanov S, Martínez-Solano I, Montgomery R, Naumov B, O’Neill M, North A, Pabijan M, Pushendorf R, Salvi D, Schmidt B, Sotiropoulos K, Stanescu F, Stanković D, Stapleton S, Šunje E, Szabolcs M, Vacheva E, Willis D, Zimić A, France J, Meilink WR, Stark T, Struijk RP, Theodoropoulos A, de Visser MC, Wielstra B. An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations. in Amphibia-Reptilia. 2023;44(3):347-361.
doi:10.1163/15685381-bja10144 .

Robbemont, Jody, van Veldhuijzen, Sam, Allain, Steven J.R., Ambu, Johanna, Boyle, Ryan, Canestrelli, Daniele, Cathasaigh, Éinne Ó, Cathrine, Chris, Chiocchio, Andrea, Cogalniceanu, Dan, Cvijanović, Milena, Dufresnes, Christophe, Ennis, Collie, Gandola, Rob, Jablonski, Daniel, Julian, Angela, Kranželić, Daria, Lukanov, Simeon, Martínez-Solano, Iñigo, Montgomery, Ryan, Naumov, Borislav, O’Neill, Matthew, North, Alexandra, Pabijan, Maciej, Pushendorf, Robert, Salvi, Daniele, Schmidt, Bruno, Sotiropoulos, Konstantinos, Stanescu, Florina, Stanković, David, Stapleton, Sarah, Šunje, Emina, Szabolcs, Márton, Vacheva, Emiliya, Willis, David, Zimić, Adnan, France, James, Meilink, Willem R.M., Stark, Tariq, Struijk, Richard P.J.H., Theodoropoulos, Anagnostis, de Visser, Manon C., Wielstra, Ben, "An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations" in Amphibia-Reptilia, 44, no. 3 (2023):347-361,
https://doi.org/10.1163/15685381-bja10144 . .

TY  - JOUR
AU  - Anđelković, Marko
AU  - Mirč, Marko
AU  - Ajduković, Maja
AU  - Cvijanović, Milena
AU  - Vukov, Tanja
AU  - Vučić, Tijana
AU  - Kijanović, Ana
AU  - Urošević, Aleksandar
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5519
AB  - Alpine habitats are exposed to increasing anthropogenic pressure and climate change. The negative impacts can lead to chronic stress that can affect the survival and reproductive success of individuals and even lead to population extinction. In this study, we analyse different morphological and ecological traits and indices of abiotic and biotic stressors (such as head size and shape, fluctuating asymmetry, body condition index, tail autotomy, and population abundance) in alpine and subalpine populations of two lacertid species (Zootoca vivipara and Lacerta agilis) from Serbia and North Macedonia. These lizards live under different conditions: allotopy/syntopy, different anthropogenic pressure, and different levels of habitat protection. We found differences between syntopic and allotopic populations in pileus size, body condition index (in both species), pileus shape, fluctuating asymmetry (in L. agilis), and abundance (in Z. vivipara). Differences between populations under anthropogenic pressure and populations without it were observed in pileus shape, body condition index (in both species), pileus size, fluctuating asymmetry, tail autotomy and abundance (in L. agilis). On the basis of our results, it is necessary to include other stress indicators in addition to fluctuating asymmetry to quickly observe and quantify the negative effects of threat factors and apply protective measures.
PB  - Basel: MDPI
T2  - Symmetry
T1  - Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?
IS  - 3
VL  - 15
DO  - 10.3390/sym15030721
SP  - 721
ER  - 

@article{
author = "Anđelković, Marko and Mirč, Marko and Ajduković, Maja and Cvijanović, Milena and Vukov, Tanja and Vučić, Tijana and Kijanović, Ana and Urošević, Aleksandar",
year = "2023",
abstract = "Alpine habitats are exposed to increasing anthropogenic pressure and climate change. The negative impacts can lead to chronic stress that can affect the survival and reproductive success of individuals and even lead to population extinction. In this study, we analyse different morphological and ecological traits and indices of abiotic and biotic stressors (such as head size and shape, fluctuating asymmetry, body condition index, tail autotomy, and population abundance) in alpine and subalpine populations of two lacertid species (Zootoca vivipara and Lacerta agilis) from Serbia and North Macedonia. These lizards live under different conditions: allotopy/syntopy, different anthropogenic pressure, and different levels of habitat protection. We found differences between syntopic and allotopic populations in pileus size, body condition index (in both species), pileus shape, fluctuating asymmetry (in L. agilis), and abundance (in Z. vivipara). Differences between populations under anthropogenic pressure and populations without it were observed in pileus shape, body condition index (in both species), pileus size, fluctuating asymmetry, tail autotomy and abundance (in L. agilis). On the basis of our results, it is necessary to include other stress indicators in addition to fluctuating asymmetry to quickly observe and quantify the negative effects of threat factors and apply protective measures.",
publisher = "Basel: MDPI",
journal = "Symmetry",
title = "Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?",
number = "3",
volume = "15",
doi = "10.3390/sym15030721",
pages = "721"
}

Anđelković, M., Mirč, M., Ajduković, M., Cvijanović, M., Vukov, T., Vučić, T., Kijanović, A.,& Urošević, A.. (2023). Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?. in Symmetry
Basel: MDPI., 15(3), 721.
https://doi.org/10.3390/sym15030721

Anđelković M, Mirč M, Ajduković M, Cvijanović M, Vukov T, Vučić T, Kijanović A, Urošević A. Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?. in Symmetry. 2023;15(3):721.
doi:10.3390/sym15030721 .

Anđelković, Marko, Mirč, Marko, Ajduković, Maja, Cvijanović, Milena, Vukov, Tanja, Vučić, Tijana, Kijanović, Ana, Urošević, Aleksandar, "Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?" in Symmetry, 15, no. 3 (2023):721,
https://doi.org/10.3390/sym15030721 . .

TY  - JOUR
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Arntzen, Jan W.
PY  - 2023
UR  - https://link.springer.com/10.1007/s13127-022-00585-5
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5167
AB  - We examined the cranial morphology and cranial kinesis of the common toads Bufo bufo and B. spinosus with micro-computed tomography and geometric morphometrics and compared the results with published data for related species in a phylogenetic context. The species significantly diverge in skull shape. The skull of B. spinosus is shorter and higher, with a ventral arm of the squamosal bone and the jaw articulation point positioned perpendicular to the braincase, in comparison with a more lateral position in B. bufo . In either species, females have a shorter snout and a higher and wider skull at the jaw articulation point that is positioned more posteriorly, in comparison with conspecific males. High variation in the amount of bone ossification was recorded in both species, ranging from scarcely ossified and loosely connected bones to highly ossified and firmly connected bones. We also found that skull shape and inferred kinetic properties of the skull are highly variable across the Bufonini tribe. However, sample sizes are mostly small and intraspecific variation is high, which might compromise the analyses. Overall, the results suggest that developmental plasticity produces high variation in ossification and cranial kinesis, affecting individuals’ feeding performances. At the population level, this variation supports an efficient exploitation of the habitat and may promote morphological adaptation in a changing environment.
PB  - Heidelberg: Springer Heidelberg
T2  - Organisms Diversity & Evolution
T1  - Differentiation of skull morphology and cranial kinesis in common toads
VL  - 23
DO  - 10.1007/s13127-022-00585-5
SP  - 209
EP  - 219
ER  - 

@article{
author = "Ivanović, Ana and Cvijanović, Milena and Vučić, Tijana and Arntzen, Jan W.",
year = "2023",
abstract = "We examined the cranial morphology and cranial kinesis of the common toads Bufo bufo and B. spinosus with micro-computed tomography and geometric morphometrics and compared the results with published data for related species in a phylogenetic context. The species significantly diverge in skull shape. The skull of B. spinosus is shorter and higher, with a ventral arm of the squamosal bone and the jaw articulation point positioned perpendicular to the braincase, in comparison with a more lateral position in B. bufo . In either species, females have a shorter snout and a higher and wider skull at the jaw articulation point that is positioned more posteriorly, in comparison with conspecific males. High variation in the amount of bone ossification was recorded in both species, ranging from scarcely ossified and loosely connected bones to highly ossified and firmly connected bones. We also found that skull shape and inferred kinetic properties of the skull are highly variable across the Bufonini tribe. However, sample sizes are mostly small and intraspecific variation is high, which might compromise the analyses. Overall, the results suggest that developmental plasticity produces high variation in ossification and cranial kinesis, affecting individuals’ feeding performances. At the population level, this variation supports an efficient exploitation of the habitat and may promote morphological adaptation in a changing environment.",
publisher = "Heidelberg: Springer Heidelberg",
journal = "Organisms Diversity & Evolution",
title = "Differentiation of skull morphology and cranial kinesis in common toads",
volume = "23",
doi = "10.1007/s13127-022-00585-5",
pages = "209-219"
}

Ivanović, A., Cvijanović, M., Vučić, T.,& Arntzen, J. W.. (2023). Differentiation of skull morphology and cranial kinesis in common toads. in Organisms Diversity & Evolution
Heidelberg: Springer Heidelberg., 23, 209-219.
https://doi.org/10.1007/s13127-022-00585-5

Ivanović A, Cvijanović M, Vučić T, Arntzen JW. Differentiation of skull morphology and cranial kinesis in common toads. in Organisms Diversity & Evolution. 2023;23:209-219.
doi:10.1007/s13127-022-00585-5 .

Ivanović, Ana, Cvijanović, Milena, Vučić, Tijana, Arntzen, Jan W., "Differentiation of skull morphology and cranial kinesis in common toads" in Organisms Diversity & Evolution, 23 (2023):209-219,
https://doi.org/10.1007/s13127-022-00585-5 . .

TY  - JOUR
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Arntzen, Jan Willem
AU  - Ivanović, Ana
AU  - Vučić, Tijana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5474
AB  - Micro-computed tomography is a powerful tool toward the detailed reconstruction of internal and external morphology, in particular for ossified and other dense tissues. Here, we document and compare the level of calcification in the skin of the head and the parotoids (the external skin glands) in males and females of common and spined toads, Bufo bufo and B. spinosus. In some anurans, including Bufo species, a specific acellular calcified tissue layer within the dermis has been documented (the Eberth-Katschenko, or EK-layer). By a combination of micro-computed tomography and classical histology, we detected additional calcium deposits located in the dermal layer stratum spongiosum, positioned above the EK-layer. We showed that the level of calcification and the presence of additional calcium deposits are size and sex related, increasing in the order B. bufo males, B. spinosus males, B. bufo females to B. spinosus females. The last of these groups is the least variable. Bufo spinosus females have dense calcium deposits in the parotoids and the dorsal and ventral skin. Three-dimensional volume renderings and cross-sectional slices obtained by micro-CT scanning indicate that this approach is a promising technique for further studies on bufonid skin anatomy and geographic variation in skin calcification.
PB  - John Wiley and Sons Inc
T2  - The Anatomical Record
T1  - Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)
DO  - 10.1002/ar.25170
ER  - 

@article{
author = "Cvijanović, Milena and Ajduković, Maja and Arntzen, Jan Willem and Ivanović, Ana and Vučić, Tijana",
year = "2023",
abstract = "Micro-computed tomography is a powerful tool toward the detailed reconstruction of internal and external morphology, in particular for ossified and other dense tissues. Here, we document and compare the level of calcification in the skin of the head and the parotoids (the external skin glands) in males and females of common and spined toads, Bufo bufo and B. spinosus. In some anurans, including Bufo species, a specific acellular calcified tissue layer within the dermis has been documented (the Eberth-Katschenko, or EK-layer). By a combination of micro-computed tomography and classical histology, we detected additional calcium deposits located in the dermal layer stratum spongiosum, positioned above the EK-layer. We showed that the level of calcification and the presence of additional calcium deposits are size and sex related, increasing in the order B. bufo males, B. spinosus males, B. bufo females to B. spinosus females. The last of these groups is the least variable. Bufo spinosus females have dense calcium deposits in the parotoids and the dorsal and ventral skin. Three-dimensional volume renderings and cross-sectional slices obtained by micro-CT scanning indicate that this approach is a promising technique for further studies on bufonid skin anatomy and geographic variation in skin calcification.",
publisher = "John Wiley and Sons Inc",
journal = "The Anatomical Record",
title = "Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)",
doi = "10.1002/ar.25170"
}

Cvijanović, M., Ajduković, M., Arntzen, J. W., Ivanović, A.,& Vučić, T.. (2023). Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo). in The Anatomical Record
John Wiley and Sons Inc..
https://doi.org/10.1002/ar.25170

Cvijanović M, Ajduković M, Arntzen JW, Ivanović A, Vučić T. Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo). in The Anatomical Record. 2023;.
doi:10.1002/ar.25170 .

Cvijanović, Milena, Ajduković, Maja, Arntzen, Jan Willem, Ivanović, Ana, Vučić, Tijana, "Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)" in The Anatomical Record (2023),
https://doi.org/10.1002/ar.25170 . .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5032
AB  - Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.
PB  - Munich: Elsevier GmbH
T2  - Zoology
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
VL  - 155
DO  - 10.1016/j.zool.2022.126050
SP  - 126050
ER  - 

@article{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.",
publisher = "Munich: Elsevier GmbH",
journal = "Zoology",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
volume = "155",
doi = "10.1016/j.zool.2022.126050",
pages = "126050"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology
Munich: Elsevier GmbH., 155, 126050.
https://doi.org/10.1016/j.zool.2022.126050

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology. 2022;155:126050.
doi:10.1016/j.zool.2022.126050 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Zoology, 155 (2022):126050,
https://doi.org/10.1016/j.zool.2022.126050 . .

TY  - JOUR
AU  - Bugarčić, Marko
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
PY  - 2022
UR  - https://brill.com/view/journals/amre/aop/article-10.1163-15685381-bja10082/article-10.1163-15685381-bja10082.xml
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4873
AB  - The Macedonian crested newt T. macedonicus forms a complex hybrid zone with the Balkan crested newt T. ivanbureschi in the central part of the Balkan Peninsula. In this study, we compared life history parameters (sex ratio, survival rate and age of sexual maturation) and growth parameters (body length and mass) of T. macedonicus and T. macedonicus × T. ivanbureschi hybrids over the first three postmetamorphic years. Survival rates were high and similar for both genotypes. Sexual dimorphism in body size (length and mass) was evident at the beginning of the third postmetamorphic year, after the first breeding year, for both T. macedonicus and hybrids. We did not find clear and consistent difference in any of the analysed traits suggesting that hybridization with T. ivabureschi does not affect postmetamorphic growth and survival of T. macedonicus .
PB  - Brill Academic Publishers
T2  - Amphibia-Reptilia
T1  - Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus
IS  - 5
VL  - 10
DO  - 10.1163/15685381-bja10082
SP  - 1
EP  - 7
ER  - 

@article{
author = "Bugarčić, Marko and Ivanović, Ana and Cvijanović, Milena and Vučić, Tijana",
year = "2022",
abstract = "The Macedonian crested newt T. macedonicus forms a complex hybrid zone with the Balkan crested newt T. ivanbureschi in the central part of the Balkan Peninsula. In this study, we compared life history parameters (sex ratio, survival rate and age of sexual maturation) and growth parameters (body length and mass) of T. macedonicus and T. macedonicus × T. ivanbureschi hybrids over the first three postmetamorphic years. Survival rates were high and similar for both genotypes. Sexual dimorphism in body size (length and mass) was evident at the beginning of the third postmetamorphic year, after the first breeding year, for both T. macedonicus and hybrids. We did not find clear and consistent difference in any of the analysed traits suggesting that hybridization with T. ivabureschi does not affect postmetamorphic growth and survival of T. macedonicus .",
publisher = "Brill Academic Publishers",
journal = "Amphibia-Reptilia",
title = "Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus",
number = "5",
volume = "10",
doi = "10.1163/15685381-bja10082",
pages = "1-7"
}

Bugarčić, M., Ivanović, A., Cvijanović, M.,& Vučić, T.. (2022). Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus. in Amphibia-Reptilia
Brill Academic Publishers., 10(5), 1-7.
https://doi.org/10.1163/15685381-bja10082

Bugarčić M, Ivanović A, Cvijanović M, Vučić T. Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus. in Amphibia-Reptilia. 2022;10(5):1-7.
doi:10.1163/15685381-bja10082 .

Bugarčić, Marko, Ivanović, Ana, Cvijanović, Milena, Vučić, Tijana, "Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus" in Amphibia-Reptilia, 10, no. 5 (2022):1-7,
https://doi.org/10.1163/15685381-bja10082 . .

TY  - CONF
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5227
AB  - Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5227
ER  - 

@conference{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5227"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):41,
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Ivanović, Ana
AU  - Ajduković, Maja
AU  - Bajler, Nikola
AU  - Cvijanović, Milena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4866
AB  - Two large-bodied newt species, Triturus ivanbureschi and T. macedonicus, hybridize in nature across the Balkan Peninsula. Consequences of hybridization upon secondary contact of two species include species displacement and asymmetrical introgression of T. ivanbureschi mtDNA. We set an experimental reciprocal cross of parental species and obtained two genotypes of F1 hybrids (with T. ivanbureschi or T. macedonicus mtDNA). When hybrids attained sexual maturity, they were engaged in mutual crossings and backcrossing with parental species. We followed reproductive traits over two successive years. Our main aim was to explore the reproductive success of F1 females carrying different parental mtDNA. Additionally, we tested for differences in reproductive success within female genotypes depending on the crossing with various male genotypes (hybrids or parental species). Both female genotypes had similar oviposition periods, number of laid eggs and hatched larvae but different body and egg sizes. Overall reproductive success (percentage of egg-laying females and viability of embryos) was similar for both genotypes. The type of crossing led to some differences in reproductive success within female genotypes. The obtained results suggest that processes that led to exclusion of T. macedonicus mtDNA in natural populations may be related to the survival at postembryonic stages of F2 generation or reproductive barriers that emerged in subsequent hybrid generations.
PB  - Basel: MDPI
T2  - Animals
T1  - The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)
IS  - 4
VL  - 12
DO  - 10.3390/ani12040443
SP  - 443
ER  - 

@article{
author = "Vučić, Tijana and Ivanović, Ana and Ajduković, Maja and Bajler, Nikola and Cvijanović, Milena",
year = "2022",
abstract = "Two large-bodied newt species, Triturus ivanbureschi and T. macedonicus, hybridize in nature across the Balkan Peninsula. Consequences of hybridization upon secondary contact of two species include species displacement and asymmetrical introgression of T. ivanbureschi mtDNA. We set an experimental reciprocal cross of parental species and obtained two genotypes of F1 hybrids (with T. ivanbureschi or T. macedonicus mtDNA). When hybrids attained sexual maturity, they were engaged in mutual crossings and backcrossing with parental species. We followed reproductive traits over two successive years. Our main aim was to explore the reproductive success of F1 females carrying different parental mtDNA. Additionally, we tested for differences in reproductive success within female genotypes depending on the crossing with various male genotypes (hybrids or parental species). Both female genotypes had similar oviposition periods, number of laid eggs and hatched larvae but different body and egg sizes. Overall reproductive success (percentage of egg-laying females and viability of embryos) was similar for both genotypes. The type of crossing led to some differences in reproductive success within female genotypes. The obtained results suggest that processes that led to exclusion of T. macedonicus mtDNA in natural populations may be related to the survival at postembryonic stages of F2 generation or reproductive barriers that emerged in subsequent hybrid generations.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)",
number = "4",
volume = "12",
doi = "10.3390/ani12040443",
pages = "443"
}

Vučić, T., Ivanović, A., Ajduković, M., Bajler, N.,& Cvijanović, M.. (2022). The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae). in Animals
Basel: MDPI., 12(4), 443.
https://doi.org/10.3390/ani12040443

Vučić T, Ivanović A, Ajduković M, Bajler N, Cvijanović M. The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae). in Animals. 2022;12(4):443.
doi:10.3390/ani12040443 .

Vučić, Tijana, Ivanović, Ana, Ajduković, Maja, Bajler, Nikola, Cvijanović, Milena, "The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)" in Animals, 12, no. 4 (2022):443,
https://doi.org/10.3390/ani12040443 . .

TY  - CONF
AU  - Vučić, Tijana
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Wielstra, Ben
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5588
AB  - Large-bodied newts (Triturus sp.) represent a good model system for evo-devo studies. Females lay relatively large eggs, which are protected by transparent jelly layer, enabling easily observation of external anatomical features throughout embryonic development – from fertilized egg to fully formed larvae. MicroCT scanning aids another, more in depth insight into the development. Virtual cross sections enable visualization of internal anatomy, while volume renderings and reconstructions of 3D models enable exploration of development of internal organs and external morphology. Throughout Triturus embryonic development, homozygous embryos die due to a balanced lethal system known as chromosome 1 syndrome, which involves two non-recombining types of chromosome 1 (1A and 1B). It is hypothesized that the two potential homozygous genotypes (1A1A and 1B1B) arrest at different embryonic stages and express different phenotypes. Hence, knowledge of the embryo’s genotype is crucial for understanding normal embryonic development of heterozygous embryos and malformations which lead to lethality in homozygous embryos. In a pilot project, we tested and optimized a protocol for Triturus embryo scanning which allowed exploration of external and internal morphology while preserving the DNA. Our protocol provides an opportunity to further explore embryonic development in Triturus in diverse research lines: 1) normal embryonic development as a base line for further studies; 2) arrested development to uncover the basis of lethality; and 3) testing various evo-devo hypotheses.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Insights from the inside: 3D embryonic development of Triturus newts
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5588
ER  - 

@conference{
author = "Vučić, Tijana and Ivanović, Ana and Cvijanović, Milena and Wielstra, Ben",
year = "2022",
abstract = "Large-bodied newts (Triturus sp.) represent a good model system for evo-devo studies. Females lay relatively large eggs, which are protected by transparent jelly layer, enabling easily observation of external anatomical features throughout embryonic development – from fertilized egg to fully formed larvae. MicroCT scanning aids another, more in depth insight into the development. Virtual cross sections enable visualization of internal anatomy, while volume renderings and reconstructions of 3D models enable exploration of development of internal organs and external morphology. Throughout Triturus embryonic development, homozygous embryos die due to a balanced lethal system known as chromosome 1 syndrome, which involves two non-recombining types of chromosome 1 (1A and 1B). It is hypothesized that the two potential homozygous genotypes (1A1A and 1B1B) arrest at different embryonic stages and express different phenotypes. Hence, knowledge of the embryo’s genotype is crucial for understanding normal embryonic development of heterozygous embryos and malformations which lead to lethality in homozygous embryos. In a pilot project, we tested and optimized a protocol for Triturus embryo scanning which allowed exploration of external and internal morphology while preserving the DNA. Our protocol provides an opportunity to further explore embryonic development in Triturus in diverse research lines: 1) normal embryonic development as a base line for further studies; 2) arrested development to uncover the basis of lethality; and 3) testing various evo-devo hypotheses.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Insights from the inside: 3D embryonic development of Triturus newts",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5588"
}

Vučić, T., Ivanović, A., Cvijanović, M.,& Wielstra, B.. (2022). Insights from the inside: 3D embryonic development of Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 40.
https://hdl.handle.net/21.15107/rcub_ibiss_5588

Vučić T, Ivanović A, Cvijanović M, Wielstra B. Insights from the inside: 3D embryonic development of Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:40.
https://hdl.handle.net/21.15107/rcub_ibiss_5588 .

Vučić, Tijana, Ivanović, Ana, Cvijanović, Milena, Wielstra, Ben, "Insights from the inside: 3D embryonic development of Triturus newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):40,
https://hdl.handle.net/21.15107/rcub_ibiss_5588 .

TY  - CONF
AU  - Drobnjaković, Marija
AU  - Ivanović, Ana
AU  - Bugarčić, Marko
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Vučić, Tijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5230
AB  - Staging tables based on the external morphological characteristics are important 
for the needs of comparative embryological studies as they represent a baseline. Large bodied newts (Triturus spp.) have a transparent mucoid capsule that enables insight 
into the external morphology of the embryo during development. Triturus newts are 
characterized by the presence of chromosome 1 syndrome where about 50% of the 
offspring stops developing and dies during the mid embryonic period on the tail bud 
stage. In this study, we focused on the normal development of Balkan crested newt 
(Triturus ivanbureschi). During the breeding season animals were kept in the yard of 
the institute, in plastic tubs (from 200 to 400 liters), covered with a mosquito net that 
allowed natural day-night regime. Plastic strips were provided as an imitation of 
underwater vegetation, on which the females laid their eggs. Eggs were collected every 
morning and moved to Petri dishes at the controlled experimental conditions. The 
embryos were kept in the laboratory at a temperature of 20°C. Photographs and time lapse recordings of developing embryos were taken every day at the same time. 
Compared to already published staging tables for newts and based on the appearance 
of certain morphological structures, such as gills, extremities, balancers, etc., we
distinguished 37 different stages of normal embryonic development of T. ivanbureshi. 
Still, differences in external morphology due to the balanced lethal chromosome 1 
syndrome need further research
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Developmental stages of Triturus ivanbureschi
VL  - 260
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5230
ER  - 

@conference{
author = "Drobnjaković, Marija and Ivanović, Ana and Bugarčić, Marko and Cvijanović, Milena and Ajduković, Maja and Vučić, Tijana",
year = "2022",
abstract = "Staging tables based on the external morphological characteristics are important 
for the needs of comparative embryological studies as they represent a baseline. Large bodied newts (Triturus spp.) have a transparent mucoid capsule that enables insight 
into the external morphology of the embryo during development. Triturus newts are 
characterized by the presence of chromosome 1 syndrome where about 50% of the 
offspring stops developing and dies during the mid embryonic period on the tail bud 
stage. In this study, we focused on the normal development of Balkan crested newt 
(Triturus ivanbureschi). During the breeding season animals were kept in the yard of 
the institute, in plastic tubs (from 200 to 400 liters), covered with a mosquito net that 
allowed natural day-night regime. Plastic strips were provided as an imitation of 
underwater vegetation, on which the females laid their eggs. Eggs were collected every 
morning and moved to Petri dishes at the controlled experimental conditions. The 
embryos were kept in the laboratory at a temperature of 20°C. Photographs and time lapse recordings of developing embryos were taken every day at the same time. 
Compared to already published staging tables for newts and based on the appearance 
of certain morphological structures, such as gills, extremities, balancers, etc., we
distinguished 37 different stages of normal embryonic development of T. ivanbureshi. 
Still, differences in external morphology due to the balanced lethal chromosome 1 
syndrome need further research",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Developmental stages of Triturus ivanbureschi",
volume = "260",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5230"
}

Drobnjaković, M., Ivanović, A., Bugarčić, M., Cvijanović, M., Ajduković, M.,& Vučić, T.. (2022). Developmental stages of Triturus ivanbureschi. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 260.
https://hdl.handle.net/21.15107/rcub_ibiss_5230

Drobnjaković M, Ivanović A, Bugarčić M, Cvijanović M, Ajduković M, Vučić T. Developmental stages of Triturus ivanbureschi. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;260.
https://hdl.handle.net/21.15107/rcub_ibiss_5230 .

Drobnjaković, Marija, Ivanović, Ana, Bugarčić, Marko, Cvijanović, Milena, Ajduković, Maja, Vučić, Tijana, "Developmental stages of Triturus ivanbureschi" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia, 260 (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5230 .

TY  - CONF
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Urošević, Aleksandar
AU  - Cvijanović, Milena
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
AU  - Kijanović, Ana
AU  - Vučić, Tijana
AU  - Vukov, Tanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5229
AB  - National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5229
ER  - 

@conference{
author = "Mirč, Marko and Anđelković, Marko and Urošević, Aleksandar and Cvijanović, Milena and Tomašević Kolarov, Nataša and Ajduković, Maja and Kijanović, Ana and Vučić, Tijana and Vukov, Tanja",
year = "2022",
abstract = "National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5229"
}

Mirč, M., Anđelković, M., Urošević, A., Cvijanović, M., Tomašević Kolarov, N., Ajduković, M., Kijanović, A., Vučić, T.,& Vukov, T.. (2022). Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229

Mirč M, Anđelković M, Urošević A, Cvijanović M, Tomašević Kolarov N, Ajduković M, Kijanović A, Vučić T, Vukov T. Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

Mirč, Marko, Anđelković, Marko, Urošević, Aleksandar, Cvijanović, Milena, Tomašević Kolarov, Nataša, Ajduković, Maja, Kijanović, Ana, Vučić, Tijana, Vukov, Tanja, "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):79,
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

TY  - CONF
AU  - Bugarčić, Marko
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Wielstra, Ben
AU  - Vučić, Tijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5228
AB  - In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5228
ER  - 

@conference{
author = "Bugarčić, Marko and Ivanović, Ana and Cvijanović, Milena and Ajduković, Maja and Wielstra, Ben and Vučić, Tijana",
year = "2022",
abstract = "In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5228"
}

Bugarčić, M., Ivanović, A., Cvijanović, M., Ajduković, M., Wielstra, B.,& Vučić, T.. (2022). Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228

Bugarčić M, Ivanović A, Cvijanović M, Ajduković M, Wielstra B, Vučić T. Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

Bugarčić, Marko, Ivanović, Ana, Cvijanović, Milena, Ajduković, Maja, Wielstra, Ben, Vučić, Tijana, "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

TY  - CONF
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Ivanović, Ana
AU  - Arntzen, Jan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5134
AB  - In the skin of many terrestrial anuran taxa, a specific acellular mineralized tissue
layer (the Eberth-Katschenko layer) has been documented. This layer is generally
positioned in between the stratum spongiosum and the stratum compactum of the
dermis and has a role in reducing water loss. Here, we document and compare the
amount of calcium deposition in the skin of the head and the parotoids (the external
skin glands) in males and females of the common toad Bufo bufo and the spined toad B.
spinosus. Bufo bufo and B. spinosus are morphologically similar and both show a
conspicuous sexual dimorphism. Using standard histological techniques, we detected
calcium as an amorphous ‘ground substance’ located in the stratum spongiosum, just
above the Eberth-Katschenko layer. We observed large variability in the number of
calcium deposits between the species and the sexes. Using micro-computed
tomography (micro-CT) we were able to quantify the level of the toad skin calcification.
Bufo spinosus females stand out compared to conspecific males and B. bufo on account
of a strong calcification of the dorsal and ventral skin and the parotoid glands. Species
and sexes significantly differed in the amount of calcium deposits (Fisher’s exact test,
p < 0.001) and pairwise comparisons showed that groups differed from one another (p
< 0.05), suggesting size and sexual dimorphism in these traits. We conclude that microCT scanning is useful for the quantification of calcified structures in the anuran skin,
and keeps a promise for further studies on taxonomic and geographic variation.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5134
ER  - 

@conference{
author = "Cvijanović, Milena and Ajduković, Maja and Ivanović, Ana and Arntzen, Jan",
year = "2022",
abstract = "In the skin of many terrestrial anuran taxa, a specific acellular mineralized tissue
layer (the Eberth-Katschenko layer) has been documented. This layer is generally
positioned in between the stratum spongiosum and the stratum compactum of the
dermis and has a role in reducing water loss. Here, we document and compare the
amount of calcium deposition in the skin of the head and the parotoids (the external
skin glands) in males and females of the common toad Bufo bufo and the spined toad B.
spinosus. Bufo bufo and B. spinosus are morphologically similar and both show a
conspicuous sexual dimorphism. Using standard histological techniques, we detected
calcium as an amorphous ‘ground substance’ located in the stratum spongiosum, just
above the Eberth-Katschenko layer. We observed large variability in the number of
calcium deposits between the species and the sexes. Using micro-computed
tomography (micro-CT) we were able to quantify the level of the toad skin calcification.
Bufo spinosus females stand out compared to conspecific males and B. bufo on account
of a strong calcification of the dorsal and ventral skin and the parotoid glands. Species
and sexes significantly differed in the amount of calcium deposits (Fisher’s exact test,
p < 0.001) and pairwise comparisons showed that groups differed from one another (p
< 0.05), suggesting size and sexual dimorphism in these traits. We conclude that microCT scanning is useful for the quantification of calcified structures in the anuran skin,
and keeps a promise for further studies on taxonomic and geographic variation.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5134"
}

Cvijanović, M., Ajduković, M., Ivanović, A.,& Arntzen, J.. (2022). What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_5134

Cvijanović M, Ajduković M, Ivanović A, Arntzen J. What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_5134 .

Cvijanović, Milena, Ajduković, Maja, Ivanović, Ana, Arntzen, Jan, "What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):32,
https://hdl.handle.net/21.15107/rcub_ibiss_5134 .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4251
AB  - Background
In amphibians, thyroid hormone (TH) has a profound role in cranial development, especially in ossification of the late-appearing bones and remodeling of the skull. In the present study, we explored the influence of TH deficiency on bone ossification and resulting skull shape during the ontogeny of Triturus newt hybrid larvae obtained from interspecific crosses between T. ivanbureschi and T. macedonicus.

Methods
Larvae were treated with two concentrations of thiourea (an endocrine disruptor that chemically inhibits synthesis of TH) during the midlarval and late larval periods. Morphological differences of the cranium were assessed at the end of the midlarval period (ontogenetic stage 62) and the metamorphic stage after treatment during the late larval period.

Results
There was no difference in the ossification level and shape of the skull between the experimental groups (control and two treatment concentrations) at stage 62. During the late larval period and metamorphosis, TH deficit had a significant impact on the level of bone ossification and skull shape with no differences between the two treatment concentrations of thiourea. The most pronounced differences in bone development were: the palatopterygoid failed to disintegrate into the palatal and pterygoid portions, retardation was observed in development of the maxilla, nasal and prefrontal bones and larval organization of the vomer was retained in thiourea-treated larvae.

Conclusions
This implies that deficiency of TH caused retardation in development and arrested metamorphic cranium skeletal reorganization, which resulted in divergent cranial shape compared to the control group. Our results confirmed that skull remodeling and ossification of late-appearing bones is TH–dependent, as in other studied Urodela species. Also, our results indicate that TH plays an important role in the establishment of skull shape during the ontogeny of Triturus newts, especially during the late larval period and metamorphosis, when TH concentrations reach their maximum.
PB  - London: PEERJ INC
T2  - PeerJ
T1  - Effects of thiourea on the skull of Triturus newts during ontogeny
VL  - 9
DO  - 10.7717/peerj.11535
SP  - e11535
ER  - 

@article{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena",
year = "2021",
abstract = "Background
In amphibians, thyroid hormone (TH) has a profound role in cranial development, especially in ossification of the late-appearing bones and remodeling of the skull. In the present study, we explored the influence of TH deficiency on bone ossification and resulting skull shape during the ontogeny of Triturus newt hybrid larvae obtained from interspecific crosses between T. ivanbureschi and T. macedonicus.

Methods
Larvae were treated with two concentrations of thiourea (an endocrine disruptor that chemically inhibits synthesis of TH) during the midlarval and late larval periods. Morphological differences of the cranium were assessed at the end of the midlarval period (ontogenetic stage 62) and the metamorphic stage after treatment during the late larval period.

Results
There was no difference in the ossification level and shape of the skull between the experimental groups (control and two treatment concentrations) at stage 62. During the late larval period and metamorphosis, TH deficit had a significant impact on the level of bone ossification and skull shape with no differences between the two treatment concentrations of thiourea. The most pronounced differences in bone development were: the palatopterygoid failed to disintegrate into the palatal and pterygoid portions, retardation was observed in development of the maxilla, nasal and prefrontal bones and larval organization of the vomer was retained in thiourea-treated larvae.

Conclusions
This implies that deficiency of TH caused retardation in development and arrested metamorphic cranium skeletal reorganization, which resulted in divergent cranial shape compared to the control group. Our results confirmed that skull remodeling and ossification of late-appearing bones is TH–dependent, as in other studied Urodela species. Also, our results indicate that TH plays an important role in the establishment of skull shape during the ontogeny of Triturus newts, especially during the late larval period and metamorphosis, when TH concentrations reach their maximum.",
publisher = "London: PEERJ INC",
journal = "PeerJ",
title = "Effects of thiourea on the skull of Triturus newts during ontogeny",
volume = "9",
doi = "10.7717/peerj.11535",
pages = "e11535"
}

Ajduković, M., Vučić, T.,& Cvijanović, M.. (2021). Effects of thiourea on the skull of Triturus newts during ontogeny. in PeerJ
London: PEERJ INC., 9, e11535.
https://doi.org/10.7717/peerj.11535

Ajduković M, Vučić T, Cvijanović M. Effects of thiourea on the skull of Triturus newts during ontogeny. in PeerJ. 2021;9:e11535.
doi:10.7717/peerj.11535 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, "Effects of thiourea on the skull of Triturus newts during ontogeny" in PeerJ, 9 (2021):e11535,
https://doi.org/10.7717/peerj.11535 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/1660-4601/18/18/9653
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8464833
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4483
AB  - Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.
IS  - 18
VL  - 18
DO  - 10.3390/ijerph18189653
SP  - 9653
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Petrović, Tamara and Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.",
number = "18",
volume = "18",
doi = "10.3390/ijerph18189653",
pages = "9653"
}

Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Petrović, T., Ajduković, M., Vučić, T., Cvijanović, M., Faggio, C.,& Prokić, M.. (2021). Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(18), 9653.
https://doi.org/10.3390/ijerph18189653

Gavrić J, Despotović S, Gavrilović B, Radovanović T, Petrović T, Ajduković M, Vučić T, Cvijanović M, Faggio C, Prokić M. Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health. 2021;18(18):9653.
doi:10.3390/ijerph18189653 .

Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Petrović, Tamara, Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Faggio, Caterina, Prokić, Marko, "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis." in International Journal of Environmental Research and Public Health, 18, no. 18 (2021):9653,
https://doi.org/10.3390/ijerph18189653 . .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Ivanović, Ana
AU  - Nikolić, Sonja
AU  - Jovanović, Jovana
AU  - Cvijanović, Milena
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000026V
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4036
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5220
AB  - During three consecutive years, we compared the reproductive characteristics and oviposition dynamics of two crested newt species, Triturus ivanbureschi and T. macedonicus. These two well-defined species are of special interest because of complex interactions at their contact zone, which include hybridization, species replacement and asymmetric mitochondrial DNA introgression. In common garden experiments, females were introduced to conspecific males, to males of other species and to hybrid males. We monitored the total number and size of the deposited eggs, as well as the dynamics and duration of oviposition during three consecutive years. The number of deposited eggs of Triturus species was much higher than previously reported. Triturus macedonicus lay more eggs that were of larger size in comparison to T. ivanbureschi. Also, the onset of T. macedonicus oviposition was considerably delayed compared to T. ivanbureschi. These are fundamental data, important for understanding complex species interactions in their contact zones.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Reproductive characteristics of two Triturus species (Amphibia: Caudata)
IS  - 3
VL  - 72
DO  - 10.2298/ABS200328026V
SP  - 321
EP  - 328
ER  - 

@article{
author = "Vučić, Tijana and Ivanović, Ana and Nikolić, Sonja and Jovanović, Jovana and Cvijanović, Milena",
year = "2020",
abstract = "During three consecutive years, we compared the reproductive characteristics and oviposition dynamics of two crested newt species, Triturus ivanbureschi and T. macedonicus. These two well-defined species are of special interest because of complex interactions at their contact zone, which include hybridization, species replacement and asymmetric mitochondrial DNA introgression. In common garden experiments, females were introduced to conspecific males, to males of other species and to hybrid males. We monitored the total number and size of the deposited eggs, as well as the dynamics and duration of oviposition during three consecutive years. The number of deposited eggs of Triturus species was much higher than previously reported. Triturus macedonicus lay more eggs that were of larger size in comparison to T. ivanbureschi. Also, the onset of T. macedonicus oviposition was considerably delayed compared to T. ivanbureschi. These are fundamental data, important for understanding complex species interactions in their contact zones.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Reproductive characteristics of two Triturus species (Amphibia: Caudata)",
number = "3",
volume = "72",
doi = "10.2298/ABS200328026V",
pages = "321-328"
}

Vučić, T., Ivanović, A., Nikolić, S., Jovanović, J.,& Cvijanović, M.. (2020). Reproductive characteristics of two Triturus species (Amphibia: Caudata). in Archives of Biological Sciences
Serbian Biological Society., 72(3), 321-328.
https://doi.org/10.2298/ABS200328026V

Vučić T, Ivanović A, Nikolić S, Jovanović J, Cvijanović M. Reproductive characteristics of two Triturus species (Amphibia: Caudata). in Archives of Biological Sciences. 2020;72(3):321-328.
doi:10.2298/ABS200328026V .

Vučić, Tijana, Ivanović, Ana, Nikolić, Sonja, Jovanović, Jovana, Cvijanović, Milena, "Reproductive characteristics of two Triturus species (Amphibia: Caudata)" in Archives of Biological Sciences, 72, no. 3 (2020):321-328,
https://doi.org/10.2298/ABS200328026V . .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Sibinović, Maša
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Ivanović, Ana
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/evo.13743
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3348
AB  - In vertebrates with complex, biphasic, life cycles, larvae have a distinct morphology and ecological preferences compared to metamorphosed juveniles and adults. In amphibians, abrupt and rapid metamorphic changes transform aquatic larvae to terrestrial juveniles. The main aim of this study is to test whether, relative to larval stages, metamorphosis (1) resets the pattern of variation between ontogenetic stages and species, (2) constrains intraspecific morphological variability, and (3) similar to the "hour-glass" model reduces morphological disparity. We explore postembryonic ontogenetic trajectories of head shape (from hatching to completed metamorphosis) of two well-defined, morphologically distinct Triturus newts species and their F1 hybrids. Variation in head shape is quantified and compared on two levels: dynamic (across ontogenetic stages) and static (at a particular stage). Our results show that the ontogenetic trajectories diverge early during development and continue to diverge throughout larval stages and metamorphosis. The high within-group variance and the largest disparity level (between-group variance) characterize the metamorphosed stage. Hence, our results indicate that metamorphosis does not canalize head shape variation generated during larval development and that metamorphosed phenotype is not more constrained relative to larval ones. Therefore, metamorphosis cannot be regarded as a developmental constraint, at least not for salamander head shape.
T2  - Evolution; International Journal of Organic Evolution
T1  - Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.
DO  - 10.1111/evo.13743
ER  - 

@article{
author = "Vučić, Tijana and Sibinović, Maša and Vukov, Tanja and Tomašević Kolarov, Nataša and Cvijanović, Milena and Ivanović, Ana",
year = "2019",
abstract = "In vertebrates with complex, biphasic, life cycles, larvae have a distinct morphology and ecological preferences compared to metamorphosed juveniles and adults. In amphibians, abrupt and rapid metamorphic changes transform aquatic larvae to terrestrial juveniles. The main aim of this study is to test whether, relative to larval stages, metamorphosis (1) resets the pattern of variation between ontogenetic stages and species, (2) constrains intraspecific morphological variability, and (3) similar to the "hour-glass" model reduces morphological disparity. We explore postembryonic ontogenetic trajectories of head shape (from hatching to completed metamorphosis) of two well-defined, morphologically distinct Triturus newts species and their F1 hybrids. Variation in head shape is quantified and compared on two levels: dynamic (across ontogenetic stages) and static (at a particular stage). Our results show that the ontogenetic trajectories diverge early during development and continue to diverge throughout larval stages and metamorphosis. The high within-group variance and the largest disparity level (between-group variance) characterize the metamorphosed stage. Hence, our results indicate that metamorphosis does not canalize head shape variation generated during larval development and that metamorphosed phenotype is not more constrained relative to larval ones. Therefore, metamorphosis cannot be regarded as a developmental constraint, at least not for salamander head shape.",
journal = "Evolution; International Journal of Organic Evolution",
title = "Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.",
doi = "10.1111/evo.13743"
}

Vučić, T., Sibinović, M., Vukov, T., Tomašević Kolarov, N., Cvijanović, M.,& Ivanović, A.. (2019). Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.. in Evolution; International Journal of Organic Evolution.
https://doi.org/10.1111/evo.13743

Vučić T, Sibinović M, Vukov T, Tomašević Kolarov N, Cvijanović M, Ivanović A. Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.. in Evolution; International Journal of Organic Evolution. 2019;.
doi:10.1111/evo.13743 .

Vučić, Tijana, Sibinović, Maša, Vukov, Tanja, Tomašević Kolarov, Nataša, Cvijanović, Milena, Ivanović, Ana, "Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts." in Evolution; International Journal of Organic Evolution (2019),
https://doi.org/10.1111/evo.13743 . .

TY  - CONF
AU  - Vučić, Tijana
AU  - Nikolić, Sonja
AU  - Jovanović, Jovana
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5589
AB  - We explored some of the life history traits of Triturus ivanbureschi and T. macedonicus. These two species
hybridize and form a large introgressive zone in central Balkan Peninsula which follows the specific species
displacement scenario: Triturus macedonicus widens its range and transects the range of T. ivanbureschi. One of
the important questions is whether, in the hybrid zone, one species has advantage over the other in reproduction
or survival. During three consecutive years, we compared reproductive potentials of the two species in common
garden experiments. The females of both species were introduced both to conspecific males and males of the other
species. We monitored their reproductive success: proportions of egg-laying females, the total number of deposited
eggs, and dynamics and duration of oviposition. Our results indicate that fecundity is largely influenced by
environmental factors as well as females’ affinities towards males, but specific differences in reproductive
potential between the species were not found.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Reproductive potentials of two Triturus species from their hybrid zone
SP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5589
ER  - 

@conference{
author = "Vučić, Tijana and Nikolić, Sonja and Jovanović, Jovana and Ivanović, Ana and Cvijanović, Milena",
year = "2019",
abstract = "We explored some of the life history traits of Triturus ivanbureschi and T. macedonicus. These two species
hybridize and form a large introgressive zone in central Balkan Peninsula which follows the specific species
displacement scenario: Triturus macedonicus widens its range and transects the range of T. ivanbureschi. One of
the important questions is whether, in the hybrid zone, one species has advantage over the other in reproduction
or survival. During three consecutive years, we compared reproductive potentials of the two species in common
garden experiments. The females of both species were introduced both to conspecific males and males of the other
species. We monitored their reproductive success: proportions of egg-laying females, the total number of deposited
eggs, and dynamics and duration of oviposition. Our results indicate that fecundity is largely influenced by
environmental factors as well as females’ affinities towards males, but specific differences in reproductive
potential between the species were not found.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Reproductive potentials of two Triturus species from their hybrid zone",
pages = "303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5589"
}

Vučić, T., Nikolić, S., Jovanović, J., Ivanović, A.,& Cvijanović, M.. (2019). Reproductive potentials of two Triturus species from their hybrid zone. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 303.
https://hdl.handle.net/21.15107/rcub_ibiss_5589

Vučić T, Nikolić S, Jovanović J, Ivanović A, Cvijanović M. Reproductive potentials of two Triturus species from their hybrid zone. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:303.
https://hdl.handle.net/21.15107/rcub_ibiss_5589 .

Vučić, Tijana, Nikolić, Sonja, Jovanović, Jovana, Ivanović, Ana, Cvijanović, Milena, "Reproductive potentials of two Triturus species from their hybrid zone" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):303,
https://hdl.handle.net/21.15107/rcub_ibiss_5589 .

TY  - CONF
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Ivanović, Ana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5590
AB  - The natural hybrid zone of Triturus ivanbureschi and T. macedonicus in the central Balkan Peninsula with
a specific species displacement scenario provides an excellent background for evolutionary studies. We set up a
common garden experiment for breeding and reciprocal crossing of these two species from populations out of the
hybrid zone. We collected data on life-history and morphological diversification throughout ontogeny. Our results
showed that there are no pre- or postzygotic reproductive barriers, with proven fecundity of F1 generation in all
crossings. Reproductive characteristics and survival rates were similar for both species and their hybrids.
Hybridization significantly affects morphological variation, with hybrids showing distinct tail and head
morphology compared to parental species. The head shape ontogeny from hatchling to metamorphosed stage was
used to explore postembryonic ontogenetic trajectories and to test whether metamorphosis acts as developmental
constraint. Differences in the developmental rate of the two species were found. Hybrids had intermediate values
relative to parental species. Also, obtained results revealed that metamorphosis cannot be regarded as a
developmental constraint for salamander head shape. Overall, results obtained from the common garden
experiment provided an insight into evolutionary mechanisms that lead to divergence from the common ancestral
developmental program and evolution of ontogenies in the hybrid zone.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - What Triturus newts can tell us about diversification and evolution within hybrid zone?
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5590
ER  - 

@conference{
author = "Vučić, Tijana and Cvijanović, Milena and Ivanović, Ana",
year = "2019",
abstract = "The natural hybrid zone of Triturus ivanbureschi and T. macedonicus in the central Balkan Peninsula with
a specific species displacement scenario provides an excellent background for evolutionary studies. We set up a
common garden experiment for breeding and reciprocal crossing of these two species from populations out of the
hybrid zone. We collected data on life-history and morphological diversification throughout ontogeny. Our results
showed that there are no pre- or postzygotic reproductive barriers, with proven fecundity of F1 generation in all
crossings. Reproductive characteristics and survival rates were similar for both species and their hybrids.
Hybridization significantly affects morphological variation, with hybrids showing distinct tail and head
morphology compared to parental species. The head shape ontogeny from hatchling to metamorphosed stage was
used to explore postembryonic ontogenetic trajectories and to test whether metamorphosis acts as developmental
constraint. Differences in the developmental rate of the two species were found. Hybrids had intermediate values
relative to parental species. Also, obtained results revealed that metamorphosis cannot be regarded as a
developmental constraint for salamander head shape. Overall, results obtained from the common garden
experiment provided an insight into evolutionary mechanisms that lead to divergence from the common ancestral
developmental program and evolution of ontogenies in the hybrid zone.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "What Triturus newts can tell us about diversification and evolution within hybrid zone?",
pages = "166",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5590"
}

Vučić, T., Cvijanović, M.,& Ivanović, A.. (2019). What Triturus newts can tell us about diversification and evolution within hybrid zone?. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 166.
https://hdl.handle.net/21.15107/rcub_ibiss_5590

Vučić T, Cvijanović M, Ivanović A. What Triturus newts can tell us about diversification and evolution within hybrid zone?. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:166.
https://hdl.handle.net/21.15107/rcub_ibiss_5590 .

Vučić, Tijana, Cvijanović, Milena, Ivanović, Ana, "What Triturus newts can tell us about diversification and evolution within hybrid zone?" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):166,
https://hdl.handle.net/21.15107/rcub_ibiss_5590 .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Tomović, Ljiljana
AU  - Krizmanić, Imre
AU  - Anđelković, Marko
AU  - Golubović, Ana
AU  - Maričić, Marko
AU  - Ajtić, Rastko
AU  - Ćorović, Jelena
AU  - Čubrić, Tijana
AU  - Tomašević-Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Vukov, Tanja
AU  - Jovanović, Bogdan
AU  - Vučić, Tijana
AU  - Ajduković, Maja
AU  - Tot, Ivan
AU  - Nadaždin, Bojana
AU  - Labus, Nenad
AU  - Džukić, Georg
PY  - 2018
UR  - http://www.nhmbeo.rs/издања/bulletin.65.html
UR  - http://www.nhmbeo.rs/upload/images/Glasnik/2018_a/Bulletin 2018-09.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3292
AB  - In this study, we present updated distribution data for all three brown frog species (Rana spp.) inhabiting Serbia. The data provided consists of newly collected field records and compiled data previously published in literature or via Internet. Of the three species found in Serbia, Rana dalmatina is the most widespread, present in all three altitudinal regions and all biogeographical regions. Rana graeca is confined to Mountain-valley altitudinal region in permanent fastflowing rivers or streams and it reaches its northern range boundaries in Serbia. Rana temporaria is the rarest of the three, with fragmented range restricted to the high mountains or few isolated lowland populations, and in the north-eastern Serbia, it is vicariant with R. graeca in canyons and gorges of montane rivers. The presence of Rana arvalis in Serbia is mentioned in literature but was not confirmed during the subsequent field surveys, so we consider it only as a potential species for the Serbian batrachofauna. The brown frog faunal composition of Serbia is identical to that of Albania, Bosnia and Herzegovina, Bulgaria, Greece, Macedonia and Montenegro, while it is somewhat different from that of Hungary and Romania and the most different from Croatia and Slovenia. Serbian brown frogs belong to European (R. temporaria) and South-European (R. dalmatina, R. graeca) chorotypes. All species of brown frogs present or potentially present in Serbia and their habitats are identified as great conservation priorities.
T2  - Bulletin of the Natural History Museum
T1  - Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia
VL  - 11
DO  - 10.5937/bnhmb1306090V
SP  - 227
EP  - 245
ER  - 

@article{
author = "Urošević, Aleksandar and Tomović, Ljiljana and Krizmanić, Imre and Anđelković, Marko and Golubović, Ana and Maričić, Marko and Ajtić, Rastko and Ćorović, Jelena and Čubrić, Tijana and Tomašević-Kolarov, Nataša and Cvijanović, Milena and Vukov, Tanja and Jovanović, Bogdan and Vučić, Tijana and Ajduković, Maja and Tot, Ivan and Nadaždin, Bojana and Labus, Nenad and Džukić, Georg",
year = "2018",
abstract = "In this study, we present updated distribution data for all three brown frog species (Rana spp.) inhabiting Serbia. The data provided consists of newly collected field records and compiled data previously published in literature or via Internet. Of the three species found in Serbia, Rana dalmatina is the most widespread, present in all three altitudinal regions and all biogeographical regions. Rana graeca is confined to Mountain-valley altitudinal region in permanent fastflowing rivers or streams and it reaches its northern range boundaries in Serbia. Rana temporaria is the rarest of the three, with fragmented range restricted to the high mountains or few isolated lowland populations, and in the north-eastern Serbia, it is vicariant with R. graeca in canyons and gorges of montane rivers. The presence of Rana arvalis in Serbia is mentioned in literature but was not confirmed during the subsequent field surveys, so we consider it only as a potential species for the Serbian batrachofauna. The brown frog faunal composition of Serbia is identical to that of Albania, Bosnia and Herzegovina, Bulgaria, Greece, Macedonia and Montenegro, while it is somewhat different from that of Hungary and Romania and the most different from Croatia and Slovenia. Serbian brown frogs belong to European (R. temporaria) and South-European (R. dalmatina, R. graeca) chorotypes. All species of brown frogs present or potentially present in Serbia and their habitats are identified as great conservation priorities.",
journal = "Bulletin of the Natural History Museum",
title = "Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia",
volume = "11",
doi = "10.5937/bnhmb1306090V",
pages = "227-245"
}

Urošević, A., Tomović, L., Krizmanić, I., Anđelković, M., Golubović, A., Maričić, M., Ajtić, R., Ćorović, J., Čubrić, T., Tomašević-Kolarov, N., Cvijanović, M., Vukov, T., Jovanović, B., Vučić, T., Ajduković, M., Tot, I., Nadaždin, B., Labus, N.,& Džukić, G.. (2018). Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia. in Bulletin of the Natural History Museum, 11, 227-245.
https://doi.org/10.5937/bnhmb1306090V

Urošević A, Tomović L, Krizmanić I, Anđelković M, Golubović A, Maričić M, Ajtić R, Ćorović J, Čubrić T, Tomašević-Kolarov N, Cvijanović M, Vukov T, Jovanović B, Vučić T, Ajduković M, Tot I, Nadaždin B, Labus N, Džukić G. Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia. in Bulletin of the Natural History Museum. 2018;11:227-245.
doi:10.5937/bnhmb1306090V .

Urošević, Aleksandar, Tomović, Ljiljana, Krizmanić, Imre, Anđelković, Marko, Golubović, Ana, Maričić, Marko, Ajtić, Rastko, Ćorović, Jelena, Čubrić, Tijana, Tomašević-Kolarov, Nataša, Cvijanović, Milena, Vukov, Tanja, Jovanović, Bogdan, Vučić, Tijana, Ajduković, Maja, Tot, Ivan, Nadaždin, Bojana, Labus, Nenad, Džukić, Georg, "Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia" in Bulletin of the Natural History Museum, 11 (2018):227-245,
https://doi.org/10.5937/bnhmb1306090V . .

TY  - JOUR
AU  - Wielstra, Ben
AU  - Canestrelli, Daniele
AU  - Cvijanović, Milena
AU  - Denoël, Mathieu
AU  - Fijarczyk, Anna
AU  - Jablonski, Daniel
AU  - Liana, Marcin
AU  - Naumov, Borislav
AU  - Olgun, Kurtuluş
AU  - Pabijan, Maciej
AU  - Pezzarossa, Alice
AU  - Popgeorgiev, Georgi
AU  - Salvi, Daniele
AU  - Si, Yali
AU  - Sillero, Neftalí
AU  - Sotiropoulos, Konstantinos
AU  - Zieliński, Piotr
AU  - Babik, Wiesław
PY  - 2018
UR  - http://booksandjournals.brillonline.com/content/journals/10.1163/15685381-17000128
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3041
AB  - The ‘smooth newt’, the taxon traditionally referred to as Lissotriton vulgaris, consists of multiple morphologically distinct taxa. Given the uncertainty concerning the validity and rank of these taxa, L. vulgaris sensu lato has often been treated as a single, polytypic species. A recent study, driven by genetic data, proposed to recognize five species, L. graecus, L. kosswigi, L. lantzi, L. schmidtleri and a more restricted L. vulgaris. The Carpathian newt L. montandoni was confirmed to be a closely related sister species. We propose to refer to this collective of six Lissotriton species as the smooth newt or Lissotriton vulgaris species complex. Guided by comprehensive genomic data from throughout the range of the smooth newt species complex we 1) delineate the distribution ranges, 2) provide a distribution database, and 3) produce distribution maps according to the format of the New Atlas of Amphibians and Reptiles of Europe, for the six constituent species. This allows us to 4) highlight regions where more research is needed to determine the position of contact zones.
T2  - Amphibia-Reptilia
T1  - The distributions of the six species constituting the smooth newt species complex (Lissotriton vulgaris sensu lato and L. montandoni) – an addition to the New Atlas of Amphibians and Reptiles of Europe
IS  - 2
VL  - 39
DO  - 10.1163/15685381-17000128
SP  - 252
EP  - 259
ER  - 

@article{
author = "Wielstra, Ben and Canestrelli, Daniele and Cvijanović, Milena and Denoël, Mathieu and Fijarczyk, Anna and Jablonski, Daniel and Liana, Marcin and Naumov, Borislav and Olgun, Kurtuluş and Pabijan, Maciej and Pezzarossa, Alice and Popgeorgiev, Georgi and Salvi, Daniele and Si, Yali and Sillero, Neftalí and Sotiropoulos, Konstantinos and Zieliński, Piotr and Babik, Wiesław",
year = "2018",
abstract = "The ‘smooth newt’, the taxon traditionally referred to as Lissotriton vulgaris, consists of multiple morphologically distinct taxa. Given the uncertainty concerning the validity and rank of these taxa, L. vulgaris sensu lato has often been treated as a single, polytypic species. A recent study, driven by genetic data, proposed to recognize five species, L. graecus, L. kosswigi, L. lantzi, L. schmidtleri and a more restricted L. vulgaris. The Carpathian newt L. montandoni was confirmed to be a closely related sister species. We propose to refer to this collective of six Lissotriton species as the smooth newt or Lissotriton vulgaris species complex. Guided by comprehensive genomic data from throughout the range of the smooth newt species complex we 1) delineate the distribution ranges, 2) provide a distribution database, and 3) produce distribution maps according to the format of the New Atlas of Amphibians and Reptiles of Europe, for the six constituent species. This allows us to 4) highlight regions where more research is needed to determine the position of contact zones.",
journal = "Amphibia-Reptilia",
title = "The distributions of the six species constituting the smooth newt species complex (Lissotriton vulgaris sensu lato and L. montandoni) – an addition to the New Atlas of Amphibians and Reptiles of Europe",
number = "2",
volume = "39",
doi = "10.1163/15685381-17000128",
pages = "252-259"
}

Wielstra, B., Canestrelli, D., Cvijanović, M., Denoël, M., Fijarczyk, A., Jablonski, D., Liana, M., Naumov, B., Olgun, K., Pabijan, M., Pezzarossa, A., Popgeorgiev, G., Salvi, D., Si, Y., Sillero, N., Sotiropoulos, K., Zieliński, P.,& Babik, W.. (2018). The distributions of the six species constituting the smooth newt species complex (Lissotriton vulgaris sensu lato and L. montandoni) – an addition to the New Atlas of Amphibians and Reptiles of Europe. in Amphibia-Reptilia, 39(2), 252-259.
https://doi.org/10.1163/15685381-17000128

Wielstra B, Canestrelli D, Cvijanović M, Denoël M, Fijarczyk A, Jablonski D, Liana M, Naumov B, Olgun K, Pabijan M, Pezzarossa A, Popgeorgiev G, Salvi D, Si Y, Sillero N, Sotiropoulos K, Zieliński P, Babik W. The distributions of the six species constituting the smooth newt species complex (Lissotriton vulgaris sensu lato and L. montandoni) – an addition to the New Atlas of Amphibians and Reptiles of Europe. in Amphibia-Reptilia. 2018;39(2):252-259.
doi:10.1163/15685381-17000128 .

Wielstra, Ben, Canestrelli, Daniele, Cvijanović, Milena, Denoël, Mathieu, Fijarczyk, Anna, Jablonski, Daniel, Liana, Marcin, Naumov, Borislav, Olgun, Kurtuluş, Pabijan, Maciej, Pezzarossa, Alice, Popgeorgiev, Georgi, Salvi, Daniele, Si, Yali, Sillero, Neftalí, Sotiropoulos, Konstantinos, Zieliński, Piotr, Babik, Wiesław, "The distributions of the six species constituting the smooth newt species complex (Lissotriton vulgaris sensu lato and L. montandoni) – an addition to the New Atlas of Amphibians and Reptiles of Europe" in Amphibia-Reptilia, 39, no. 2 (2018):252-259,
https://doi.org/10.1163/15685381-17000128 . .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Ivanović, Ana
PY  - 2018
UR  - http://booksandjournals.brillonline.com/content/journals/10.1163/15685381-17000190
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3013
AB  - In amphibians, morphological differentiation and disparity at the larval and post-metamorphic ontogenetic stages can diverge, owing to various contrasting environments and different selective pressures. In the monophyletic clade of nine Triturus newt species, five different morphotypes can be recognized, but information on larval morphology is limited. Here we explore divergence of larval morphology in Triturus ivanbureschi, T. macedonicus, and their F1 hybrids. These two genetically and morphologically distinct crested newt species hybridize in nature and form a relatively wide hybrid zone in the central part of the Balkan Peninsula. Using a geometric morphometric approach and multivariate statistics, we evaluated differences of tail size and shape, colouration pattern, and the presence of a tail filament at the mid-larval stage in larvae reared under controlled laboratory conditions. We chose the tail as the main propulsive organ crucial for locomotion, feeding, and escaping predators. We found that Triturus ivanbureschi and T. macedonicus larvae differ in tail shape, but not in tail size. Two groups of F1 hybrid larvae (obtained from reciprocal crossing) were similar to each other, but differed from the parental species in size and shape of the tail, colouration pattern, and the presence of a tail filament. Our results indicate that, like adults, larvae diverge morphologically and hybrid larvae do not exhibit intermediate morphology of the parental species.
T2  - Amphibia-Reptilia
T1  - The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids
IS  - 1
VL  - 39
DO  - 10.1163/15685381-17000190
SP  - 87
EP  - 97
ER  - 

@article{
author = "Vučić, Tijana and Vukov, Tanja and Tomašević Kolarov, Nataša and Cvijanović, Milena and Ivanović, Ana",
year = "2018",
abstract = "In amphibians, morphological differentiation and disparity at the larval and post-metamorphic ontogenetic stages can diverge, owing to various contrasting environments and different selective pressures. In the monophyletic clade of nine Triturus newt species, five different morphotypes can be recognized, but information on larval morphology is limited. Here we explore divergence of larval morphology in Triturus ivanbureschi, T. macedonicus, and their F1 hybrids. These two genetically and morphologically distinct crested newt species hybridize in nature and form a relatively wide hybrid zone in the central part of the Balkan Peninsula. Using a geometric morphometric approach and multivariate statistics, we evaluated differences of tail size and shape, colouration pattern, and the presence of a tail filament at the mid-larval stage in larvae reared under controlled laboratory conditions. We chose the tail as the main propulsive organ crucial for locomotion, feeding, and escaping predators. We found that Triturus ivanbureschi and T. macedonicus larvae differ in tail shape, but not in tail size. Two groups of F1 hybrid larvae (obtained from reciprocal crossing) were similar to each other, but differed from the parental species in size and shape of the tail, colouration pattern, and the presence of a tail filament. Our results indicate that, like adults, larvae diverge morphologically and hybrid larvae do not exhibit intermediate morphology of the parental species.",
journal = "Amphibia-Reptilia",
title = "The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids",
number = "1",
volume = "39",
doi = "10.1163/15685381-17000190",
pages = "87-97"
}

Vučić, T., Vukov, T., Tomašević Kolarov, N., Cvijanović, M.,& Ivanović, A.. (2018). The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids. in Amphibia-Reptilia, 39(1), 87-97.
https://doi.org/10.1163/15685381-17000190

Vučić T, Vukov T, Tomašević Kolarov N, Cvijanović M, Ivanović A. The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids. in Amphibia-Reptilia. 2018;39(1):87-97.
doi:10.1163/15685381-17000190 .

Vučić, Tijana, Vukov, Tanja, Tomašević Kolarov, Nataša, Cvijanović, Milena, Ivanović, Ana, "The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids" in Amphibia-Reptilia, 39, no. 1 (2018):87-97,
https://doi.org/10.1163/15685381-17000190 . .

TY  - CONF
AU  - Danon, Gorana
AU  - Anđelković, Marko
AU  - Cvijanović, Milena
AU  - Urošević, Aleksandar
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5416
AB  - To investigate host-parasite relations in different habitats and its influence on host fitness a
total of 132 lizards belonging to three lacertid species (Lacerta agilis, Podarcis muralis,
Podarcis tauricus) were collected at three localities in Serbia from March to June of 2017. First
locality (Hatarice, 90 m a.s.l.) is a rather preserved steppe habitat of P.tauricus, 66 km northeast
of Belgrade. Second is a montane meadow at Divčibare (980 m a.s.l.), a habitat of L. agilis,
115 km south-west from the capital. Both of them have low anthropogenic disturbance. Third
location (Reva, 70m a.s.l.) is a suburban area, just outside Belgrade, inhabited by L. agilis and
P. muralis, with a high level of anthropogenic disturbance.
Lizards were collected by noose or hand and examined for external parasites. After their
body mass and SVL were measured, they were marked and released. A total number of 117
ticks were removed and preserved. The percentage of tick infestation in every population was
determined. Lizard’s body condition index, parasite load and percentage of infestation were
calculated.
Lacerta agilis from Reva had the highest tick infestation overall (57.14%) and P. muralis from
Reva were also highly infested (42.31%). Tick infestation was low in habitats with minor
anthropogenic disturbance (Hatarice 2.38%; Divčibare 2.94%). Data analysis showed that body
condition index was not significantly correlated with parasite load. No significant differences in
infestation percentage were detected between two species found in the same habitat (Reva).
However, habitat type had a statistically significant influence on tick infestation intensity
among populations.
C3  - Programme and Abstracts: 19th European Congress of Herpetology: SEH 2017; 2017 Sep 18-23; Salzburg, Austria
T1  - Tick infestation of three lacertid lizard species from different localities in Serbia – preliminary results
SP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5416
ER  - 

@conference{
author = "Danon, Gorana and Anđelković, Marko and Cvijanović, Milena and Urošević, Aleksandar",
year = "2017",
abstract = "To investigate host-parasite relations in different habitats and its influence on host fitness a
total of 132 lizards belonging to three lacertid species (Lacerta agilis, Podarcis muralis,
Podarcis tauricus) were collected at three localities in Serbia from March to June of 2017. First
locality (Hatarice, 90 m a.s.l.) is a rather preserved steppe habitat of P.tauricus, 66 km northeast
of Belgrade. Second is a montane meadow at Divčibare (980 m a.s.l.), a habitat of L. agilis,
115 km south-west from the capital. Both of them have low anthropogenic disturbance. Third
location (Reva, 70m a.s.l.) is a suburban area, just outside Belgrade, inhabited by L. agilis and
P. muralis, with a high level of anthropogenic disturbance.
Lizards were collected by noose or hand and examined for external parasites. After their
body mass and SVL were measured, they were marked and released. A total number of 117
ticks were removed and preserved. The percentage of tick infestation in every population was
determined. Lizard’s body condition index, parasite load and percentage of infestation were
calculated.
Lacerta agilis from Reva had the highest tick infestation overall (57.14%) and P. muralis from
Reva were also highly infested (42.31%). Tick infestation was low in habitats with minor
anthropogenic disturbance (Hatarice 2.38%; Divčibare 2.94%). Data analysis showed that body
condition index was not significantly correlated with parasite load. No significant differences in
infestation percentage were detected between two species found in the same habitat (Reva).
However, habitat type had a statistically significant influence on tick infestation intensity
among populations.",
journal = "Programme and Abstracts: 19th European Congress of Herpetology: SEH 2017; 2017 Sep 18-23; Salzburg, Austria",
title = "Tick infestation of three lacertid lizard species from different localities in Serbia – preliminary results",
pages = "196",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5416"
}

Danon, G., Anđelković, M., Cvijanović, M.,& Urošević, A.. (2017). Tick infestation of three lacertid lizard species from different localities in Serbia – preliminary results. in Programme and Abstracts: 19th European Congress of Herpetology: SEH 2017; 2017 Sep 18-23; Salzburg, Austria, 196.
https://hdl.handle.net/21.15107/rcub_ibiss_5416

Danon G, Anđelković M, Cvijanović M, Urošević A. Tick infestation of three lacertid lizard species from different localities in Serbia – preliminary results. in Programme and Abstracts: 19th European Congress of Herpetology: SEH 2017; 2017 Sep 18-23; Salzburg, Austria. 2017;:196.
https://hdl.handle.net/21.15107/rcub_ibiss_5416 .

Danon, Gorana, Anđelković, Marko, Cvijanović, Milena, Urošević, Aleksandar, "Tick infestation of three lacertid lizard species from different localities in Serbia – preliminary results" in Programme and Abstracts: 19th European Congress of Herpetology: SEH 2017; 2017 Sep 18-23; Salzburg, Austria (2017):196,
https://hdl.handle.net/21.15107/rcub_ibiss_5416 .