TY  - JOUR
AU  - Nesić, Dejan M
AU  - Stevanović, Darko M
AU  - Stanković, Sanja Đ
AU  - Milošević, Verica
AU  - Trajković, Vladimir S
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/997
AB  - Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related metabolic disturbances. We evaluated the influence of age on the orexigenic and lipid metabolism-altering effects of ghrelin. Peripubertal, young, adult and middle-aged rats (1, 2, 7 and 12 months old, respectively) were treated with 5 daily intracerebroventricular injections of ghrelin (0.15 nmol) or saline (control). The food intake was measured daily before treatment, while white adipose tissue and serum/plasma samples for detection of lipid metabolites/hormones were collected at the end of the experiment. The values of cumulative food intake and body weight gain declined, while the white adipose tissue deposits and blood concentrations of triglycerides, cholesterol and free fatty acids all increased with age. Ghrelin significantly increased all parameters, but the stimulatory effects on body weight gain and food intake were more pronounced in peripubertal/young rats, while the increase in white adipose mass, triglyceride and low-density lipoprotein cholesterol levels was more noticeable in adult/middle-aged animals. The decrease in sensitivity to ghrelin-mediated stimulation of food intake in older animals could not be explained by alterations in ghrelin's ability to reduce anorexigenic hormones insulin and leptin. However, the higher responsiveness of aged rats to ghrelin-mediated increase in lipid metabolites was accompanied by an increase in adrenocorticotropic hormone and corticosterone levels. These results indicate that aging, while reducing sensitivity to ghrelin-mediated increase in body weight gain and food intake, might enhance the responsiveness to the stimulatory effects of ghrelin on lipid metabolites and hypothalamic-pituitary-adrenal axis activity. (C) 2013 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmacology
T1  - Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats
IS  - 1-3
VL  - 710
SP  - 153
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_997
ER  - 

@article{
author = "Nesić, Dejan M and Stevanović, Darko M and Stanković, Sanja Đ and Milošević, Verica and Trajković, Vladimir S and Starčević, Vesna P. and Severs, Walter B",
year = "2013",
abstract = "Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related metabolic disturbances. We evaluated the influence of age on the orexigenic and lipid metabolism-altering effects of ghrelin. Peripubertal, young, adult and middle-aged rats (1, 2, 7 and 12 months old, respectively) were treated with 5 daily intracerebroventricular injections of ghrelin (0.15 nmol) or saline (control). The food intake was measured daily before treatment, while white adipose tissue and serum/plasma samples for detection of lipid metabolites/hormones were collected at the end of the experiment. The values of cumulative food intake and body weight gain declined, while the white adipose tissue deposits and blood concentrations of triglycerides, cholesterol and free fatty acids all increased with age. Ghrelin significantly increased all parameters, but the stimulatory effects on body weight gain and food intake were more pronounced in peripubertal/young rats, while the increase in white adipose mass, triglyceride and low-density lipoprotein cholesterol levels was more noticeable in adult/middle-aged animals. The decrease in sensitivity to ghrelin-mediated stimulation of food intake in older animals could not be explained by alterations in ghrelin's ability to reduce anorexigenic hormones insulin and leptin. However, the higher responsiveness of aged rats to ghrelin-mediated increase in lipid metabolites was accompanied by an increase in adrenocorticotropic hormone and corticosterone levels. These results indicate that aging, while reducing sensitivity to ghrelin-mediated increase in body weight gain and food intake, might enhance the responsiveness to the stimulatory effects of ghrelin on lipid metabolites and hypothalamic-pituitary-adrenal axis activity. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmacology",
title = "Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats",
number = "1-3",
volume = "710",
pages = "153-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_997"
}

Nesić, D. M., Stevanović, D. M., Stanković, S. Đ., Milošević, V., Trajković, V. S., Starčević, V. P.,& Severs, W. B.. (2013). Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats. in European Journal of Pharmacology, 710(1-3), 153-91.
https://hdl.handle.net/21.15107/rcub_ibiss_997

Nesić DM, Stevanović DM, Stanković SĐ, Milošević V, Trajković VS, Starčević VP, Severs WB. Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats. in European Journal of Pharmacology. 2013;710(1-3):153-91.
https://hdl.handle.net/21.15107/rcub_ibiss_997 .

Nesić, Dejan M, Stevanović, Darko M, Stanković, Sanja Đ, Milošević, Verica, Trajković, Vladimir S, Starčević, Vesna P., Severs, Walter B, "Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats" in European Journal of Pharmacology, 710, no. 1-3 (2013):153-91,
https://hdl.handle.net/21.15107/rcub_ibiss_997 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Nesić, Dejan M
AU  - Starčević, Vesna P.
AU  - Rakocević, Rastko
AU  - Stevanović, Darko M
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1048
AB  - Changes in feeding regime represent serious stress, while ghrelin is considered a key player in energy balance. We investigated the effects of intracerebroventricular (ICV) ghrelin application on pituitary adrenocorticotropic (ACTH) cells in rats fed diets differing in energy content. Before the ICV treatment, male Wistar rats were subjected to three different feeding regimes for 4 weeks: normal-fed (NF), food-restricted (FR) or high-fed (HF) (n = 3 x 14). At the age of 8 weeks, rats from each group were divided into two subgroups and given ICV, either ghrelin (G; 1 mu g ghrelin/5 mu l PBS, n = 7) or solvent alone (5 mu l PBS, n = 7) every 24 h for 5 days. The immunohistochemical appearance and quantitative morphology of pituitary ACTH cells were evaluated, as well as peripheral ACTH and corticosterone levels. Central ghrelin administration increased (p < 0.05) ACTH cell volumes in GNF, GFR and GHF rats by 8.1%, 11.8% and 9.1%, respectively, compared to the controls, while significant increases in ACTH cell volume density were observed in GNF and GHF rats. Circulating ACTH and corticosterone levels were elevated (p < 0.05) in GNF and GFR rats by 72.8% and 80.8%, respectively, when compared to the corresponding controls. Thus, central ghrelin administration stimulated the pituitary-adrenal axis under preserved and negative energy balance states. (C) 2013 Elsevier GmbH. All rights reserved.
T2  - Acta Histochemica
T1  - Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study
IS  - 8
VL  - 115
SP  - 283
EP  - 864
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1048
ER  - 

@article{
author = "Milošević, Verica and Ajdžanović, Vladimir and Filipović, Branko and Nesić, Dejan M and Starčević, Vesna P. and Rakocević, Rastko and Stevanović, Darko M",
year = "2013",
abstract = "Changes in feeding regime represent serious stress, while ghrelin is considered a key player in energy balance. We investigated the effects of intracerebroventricular (ICV) ghrelin application on pituitary adrenocorticotropic (ACTH) cells in rats fed diets differing in energy content. Before the ICV treatment, male Wistar rats were subjected to three different feeding regimes for 4 weeks: normal-fed (NF), food-restricted (FR) or high-fed (HF) (n = 3 x 14). At the age of 8 weeks, rats from each group were divided into two subgroups and given ICV, either ghrelin (G; 1 mu g ghrelin/5 mu l PBS, n = 7) or solvent alone (5 mu l PBS, n = 7) every 24 h for 5 days. The immunohistochemical appearance and quantitative morphology of pituitary ACTH cells were evaluated, as well as peripheral ACTH and corticosterone levels. Central ghrelin administration increased (p < 0.05) ACTH cell volumes in GNF, GFR and GHF rats by 8.1%, 11.8% and 9.1%, respectively, compared to the controls, while significant increases in ACTH cell volume density were observed in GNF and GHF rats. Circulating ACTH and corticosterone levels were elevated (p < 0.05) in GNF and GFR rats by 72.8% and 80.8%, respectively, when compared to the corresponding controls. Thus, central ghrelin administration stimulated the pituitary-adrenal axis under preserved and negative energy balance states. (C) 2013 Elsevier GmbH. All rights reserved.",
journal = "Acta Histochemica",
title = "Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study",
number = "8",
volume = "115",
pages = "283-864",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1048"
}

Milošević, V., Ajdžanović, V., Filipović, B., Nesić, D. M., Starčević, V. P., Rakocević, R.,& Stevanović, D. M.. (2013). Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study. in Acta Histochemica, 115(8), 283-864.
https://hdl.handle.net/21.15107/rcub_ibiss_1048

Milošević V, Ajdžanović V, Filipović B, Nesić DM, Starčević VP, Rakocević R, Stevanović DM. Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study. in Acta Histochemica. 2013;115(8):283-864.
https://hdl.handle.net/21.15107/rcub_ibiss_1048 .

Milošević, Verica, Ajdžanović, Vladimir, Filipović, Branko, Nesić, Dejan M, Starčević, Vesna P., Rakocević, Rastko, Stevanović, Darko M, "Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study" in Acta Histochemica, 115, no. 8 (2013):283-864,
https://hdl.handle.net/21.15107/rcub_ibiss_1048 .

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Starčević, Vesna P.
AU  - Vilimanović, Uros
AU  - Nesić, Dejan M
AU  - Vučićević, Ljubica
AU  - Misirkić Marjanović, Maja
AU  - Janjetović, Kristina
AU  - Savić, Emina
AU  - Popadić, Dusan M
AU  - Sudar, Emina M
AU  - Micić, Dragan D
AU  - Sumarac-Dumanović, Mirjana S
AU  - Trajković, Vladimir S
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1238
AB  - We investigated the effects of centrally administered orexigenic hormone ghrelin on energy imbalance-induced inflammation. Rats were subjected for four weeks to three different dietary regimes: normal (standard food), high-fat (standard food with 30% lard) or food-restricted (70%, 50%, 40% and 40% of the expected food intake in 1st, 2nd, 3rd and 4th week, respectively). Compared to normal-weight controls, starved, but not obese rats had significantly higher levels of proinflammatory cytokines (TNF, IL-1 beta, IFN-gamma) in the blood. When compared to normally fed animals, the hearts of starved and obese animals expressed higher levels of mRNAs encoding proinflammatory mediators (TNF, IL-1 beta, IL-6, IFN-gamma, IL-17, IL-12, iNOS), while mRNA levels of the anti-inflammatory TGF-beta remained unchanged. Intracerebroventricular (ICV) injection of ghrelin (1 mu g/day) for five consecutive days significantly reduced TNF, IL-1 beta and IFN-gamma levels in the blood of starved rats, as well as TNF, IL-17 and IL-12p40 mRNA expression in the hearts of obese rats. Conversely, ICV ghrelin increased the levels of 1FN-gamma, IL-17,1L-12p35 and IL-12p40 mRNA in the heart tissue of food-restricted animals. This was associated with an increase of immunosuppressive ACTH/corticosterone production in starved animals and a decrease of the immunostimulatory adipokine leptin both in food-restricted and high-fat groups. Ghrelin activated the energy sensor AMP-activated protein kinase (AMPK) in the hypothalamus and inhibited extracellular signal-regulated kinase (ERK) in the hearts of obese, but not starved rats. Therefore, central ghrelin may play a complex role in energy imbalance-induced inflammation by modulating HPA axis, leptin and AMPK/ERK signaling pathways. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Brain Behavior and Immunity
T1  - Immunomodulatory actions of central ghrelin in diet-induced energy imbalance
IS  - 1
VL  - 26
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1238
ER  - 

@article{
author = "Stevanović, Darko M and Starčević, Vesna P. and Vilimanović, Uros and Nesić, Dejan M and Vučićević, Ljubica and Misirkić Marjanović, Maja and Janjetović, Kristina and Savić, Emina and Popadić, Dusan M and Sudar, Emina M and Micić, Dragan D and Sumarac-Dumanović, Mirjana S and Trajković, Vladimir S",
year = "2012",
abstract = "We investigated the effects of centrally administered orexigenic hormone ghrelin on energy imbalance-induced inflammation. Rats were subjected for four weeks to three different dietary regimes: normal (standard food), high-fat (standard food with 30% lard) or food-restricted (70%, 50%, 40% and 40% of the expected food intake in 1st, 2nd, 3rd and 4th week, respectively). Compared to normal-weight controls, starved, but not obese rats had significantly higher levels of proinflammatory cytokines (TNF, IL-1 beta, IFN-gamma) in the blood. When compared to normally fed animals, the hearts of starved and obese animals expressed higher levels of mRNAs encoding proinflammatory mediators (TNF, IL-1 beta, IL-6, IFN-gamma, IL-17, IL-12, iNOS), while mRNA levels of the anti-inflammatory TGF-beta remained unchanged. Intracerebroventricular (ICV) injection of ghrelin (1 mu g/day) for five consecutive days significantly reduced TNF, IL-1 beta and IFN-gamma levels in the blood of starved rats, as well as TNF, IL-17 and IL-12p40 mRNA expression in the hearts of obese rats. Conversely, ICV ghrelin increased the levels of 1FN-gamma, IL-17,1L-12p35 and IL-12p40 mRNA in the heart tissue of food-restricted animals. This was associated with an increase of immunosuppressive ACTH/corticosterone production in starved animals and a decrease of the immunostimulatory adipokine leptin both in food-restricted and high-fat groups. Ghrelin activated the energy sensor AMP-activated protein kinase (AMPK) in the hypothalamus and inhibited extracellular signal-regulated kinase (ERK) in the hearts of obese, but not starved rats. Therefore, central ghrelin may play a complex role in energy imbalance-induced inflammation by modulating HPA axis, leptin and AMPK/ERK signaling pathways. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Brain Behavior and Immunity",
title = "Immunomodulatory actions of central ghrelin in diet-induced energy imbalance",
number = "1",
volume = "26",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1238"
}

Stevanović, D. M., Starčević, V. P., Vilimanović, U., Nesić, D. M., Vučićević, L., Misirkić Marjanović, M., Janjetović, K., Savić, E., Popadić, D. M., Sudar, E. M., Micić, D. D., Sumarac-Dumanović, M. S.,& Trajković, V. S.. (2012). Immunomodulatory actions of central ghrelin in diet-induced energy imbalance. in Brain Behavior and Immunity, 26(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1238

Stevanović DM, Starčević VP, Vilimanović U, Nesić DM, Vučićević L, Misirkić Marjanović M, Janjetović K, Savić E, Popadić DM, Sudar EM, Micić DD, Sumarac-Dumanović MS, Trajković VS. Immunomodulatory actions of central ghrelin in diet-induced energy imbalance. in Brain Behavior and Immunity. 2012;26(1):null-158.
https://hdl.handle.net/21.15107/rcub_ibiss_1238 .

Stevanović, Darko M, Starčević, Vesna P., Vilimanović, Uros, Nesić, Dejan M, Vučićević, Ljubica, Misirkić Marjanović, Maja, Janjetović, Kristina, Savić, Emina, Popadić, Dusan M, Sudar, Emina M, Micić, Dragan D, Sumarac-Dumanović, Mirjana S, Trajković, Vladimir S, "Immunomodulatory actions of central ghrelin in diet-induced energy imbalance" in Brain Behavior and Immunity, 26, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1238 .

TY  - JOUR
AU  - Plecas-Solarović, Bosiljka A
AU  - Nesić, Dejan M
AU  - Stevanović, Darko M
AU  - Obradović, Aleksandar Lj
AU  - Đelić, Marina N
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1176
AB  - Ghrelin, the endogenous ligand of growth hormone secretagogue receptor type la (GHS-R1a), has emerged as pleiotropic modulator of diverse biological functions, including energy homeostasis and recently, reproduction. The influence of intracerebroventricularly (ICV) administered ghrelin (1 mu g/day/rat for 5 days) to rats of different ages, i.e., peripubertal (38 days), adult (60 days) and middle-aged (180 days) on the ventral prostate size and morphology, serum testosterone levels and testis weight was examined. Ghrelin treatment significantly increased (p < 0.05) absolute ventral prostate weight in peripubertal and middle-aged rats, by 27% and 37% respectively, due to enhancement of epithelial and/or luminal compartment of the gland. In adult rats, both absolute and relative volumes of the acinar lumen were significantly decreased (p < 0.05), by 38% and 44% respectively, which was associated with significant increases (p < 0.05) in relative and absolute volumes of interacinar stroma, whereas ventral prostate weigh was unchanged. Irrespective of animal age, ghrelin did not affect serum testosterone levels. These are the first results of ghrelin treatment effects on healthy prostate appearance, which allow us to conclude that the rat ventral prostate response to ghrelin depends on the developmental stage of animals. Our results merit further investigations and may have clinical implications, especially in the light of data on possible role of ghrelin in prostate hypertrophy and adenomas.
T2  - General Physiology and Biophysics
T1  - Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment
IS  - 2
VL  - 31
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1176
ER  - 

@article{
author = "Plecas-Solarović, Bosiljka A and Nesić, Dejan M and Stevanović, Darko M and Obradović, Aleksandar Lj and Đelić, Marina N and Milošević, Verica and Starčević, Vesna P.",
year = "2012",
abstract = "Ghrelin, the endogenous ligand of growth hormone secretagogue receptor type la (GHS-R1a), has emerged as pleiotropic modulator of diverse biological functions, including energy homeostasis and recently, reproduction. The influence of intracerebroventricularly (ICV) administered ghrelin (1 mu g/day/rat for 5 days) to rats of different ages, i.e., peripubertal (38 days), adult (60 days) and middle-aged (180 days) on the ventral prostate size and morphology, serum testosterone levels and testis weight was examined. Ghrelin treatment significantly increased (p < 0.05) absolute ventral prostate weight in peripubertal and middle-aged rats, by 27% and 37% respectively, due to enhancement of epithelial and/or luminal compartment of the gland. In adult rats, both absolute and relative volumes of the acinar lumen were significantly decreased (p < 0.05), by 38% and 44% respectively, which was associated with significant increases (p < 0.05) in relative and absolute volumes of interacinar stroma, whereas ventral prostate weigh was unchanged. Irrespective of animal age, ghrelin did not affect serum testosterone levels. These are the first results of ghrelin treatment effects on healthy prostate appearance, which allow us to conclude that the rat ventral prostate response to ghrelin depends on the developmental stage of animals. Our results merit further investigations and may have clinical implications, especially in the light of data on possible role of ghrelin in prostate hypertrophy and adenomas.",
journal = "General Physiology and Biophysics",
title = "Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment",
number = "2",
volume = "31",
pages = "210",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1176"
}

Plecas-Solarović, B. A., Nesić, D. M., Stevanović, D. M., Obradović, A. L., Đelić, M. N., Milošević, V.,& Starčević, V. P.. (2012). Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment. in General Physiology and Biophysics, 31(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1176

Plecas-Solarović BA, Nesić DM, Stevanović DM, Obradović AL, Đelić MN, Milošević V, Starčević VP. Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment. in General Physiology and Biophysics. 2012;31(2):null-210.
https://hdl.handle.net/21.15107/rcub_ibiss_1176 .

Plecas-Solarović, Bosiljka A, Nesić, Dejan M, Stevanović, Darko M, Obradović, Aleksandar Lj, Đelić, Marina N, Milošević, Verica, Starčević, Vesna P., "Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment" in General Physiology and Biophysics, 31, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1176 .

TY  - CONF
AU  - Brkić, Branislava
AU  - Nesić, Dejan M
AU  - Stevanović, Darko M
AU  - Đelić, Marina N
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1292
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Adiposity Effects of Centrally Administered Ghrelin in Aged Rats
IS  - null
VL  - 49
EP  - S631
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1292
ER  - 

@conference{
author = "Brkić, Branislava and Nesić, Dejan M and Stevanović, Darko M and Đelić, Marina N and Milošević, Verica and Starčević, Vesna P.",
year = "2011",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Adiposity Effects of Centrally Administered Ghrelin in Aged Rats",
number = "null",
volume = "49",
pages = "S631",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1292"
}

Brkić, B., Nesić, D. M., Stevanović, D. M., Đelić, M. N., Milošević, V.,& Starčević, V. P.. (2011). Adiposity Effects of Centrally Administered Ghrelin in Aged Rats. in Clinical Chemistry and Laboratory Medicine, 49(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1292

Brkić B, Nesić DM, Stevanović DM, Đelić MN, Milošević V, Starčević VP. Adiposity Effects of Centrally Administered Ghrelin in Aged Rats. in Clinical Chemistry and Laboratory Medicine. 2011;49(null):null-S631.
https://hdl.handle.net/21.15107/rcub_ibiss_1292 .

Brkić, Branislava, Nesić, Dejan M, Stevanović, Darko M, Đelić, Marina N, Milošević, Verica, Starčević, Vesna P., "Adiposity Effects of Centrally Administered Ghrelin in Aged Rats" in Clinical Chemistry and Laboratory Medicine, 49, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1292 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Stevanović, Darko M.
AU  - Nešić, Dejan M.
AU  - Šošić-Jurjević, Branka 
AU  - Ajdžanović, Vladimir
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1371
AB  - Ghrelin, a growth hormone secretagogue that exerts an important role in appetite and weight regulation, participates in the activation of the hypothalamo-pituitary-adrenal (HPA) axis. Male Wistar rats (5/group) received daily for 5 days, via an ICV (intracerebroventricular) cannula, 5 ill phosphate buffered saline with or without 1 mu g of rat ghrelin. Two hours after the last injection, blood and adrenal glands were collected from decapitated rats for blood hormone analyses and histologic and morphometric processing. Ghrelin treatment resulted in increased (p < 0.05) body weight (13%), absolute whole adrenal gland weight (18%) and whole adrenal gland volume (20%). The absolute volumes of the entire adrenal cortex, ZG, ZF, and ZR also increased (p < 0.05) after ghrelin by 20%, 21%, 21% and 11%, respectively. Ghrelin-treated rats had elevated (p < 0.05) blood concentrations of ACTH, aldosterone and corticosterone (68%, 32% and 67%, respectively). The data clearly provide both morphological and hormonal status that ghrelin acts centrally to exert a global stimulatory effect on the adrenal cortex. Clarifying of the ghrelin precise role in the multiple networks affecting the stress hormone release, besides its well known energy and metabolic disbalance effects, remains a very important research goal.
PB  - Slovak Academy of Sciences
T2  - General Physiology and Biophysics
T1  - Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study
IS  - 2
VL  - 29
DO  - 10.4149/gpb_2010_02_194
SP  - 194
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1371
ER  - 

@article{
author = "Milošević, Verica and Stevanović, Darko M. and Nešić, Dejan M. and Šošić-Jurjević, Branka  and Ajdžanović, Vladimir and Starčević, Vesna P. and Severs, Walter B",
year = "2010",
abstract = "Ghrelin, a growth hormone secretagogue that exerts an important role in appetite and weight regulation, participates in the activation of the hypothalamo-pituitary-adrenal (HPA) axis. Male Wistar rats (5/group) received daily for 5 days, via an ICV (intracerebroventricular) cannula, 5 ill phosphate buffered saline with or without 1 mu g of rat ghrelin. Two hours after the last injection, blood and adrenal glands were collected from decapitated rats for blood hormone analyses and histologic and morphometric processing. Ghrelin treatment resulted in increased (p < 0.05) body weight (13%), absolute whole adrenal gland weight (18%) and whole adrenal gland volume (20%). The absolute volumes of the entire adrenal cortex, ZG, ZF, and ZR also increased (p < 0.05) after ghrelin by 20%, 21%, 21% and 11%, respectively. Ghrelin-treated rats had elevated (p < 0.05) blood concentrations of ACTH, aldosterone and corticosterone (68%, 32% and 67%, respectively). The data clearly provide both morphological and hormonal status that ghrelin acts centrally to exert a global stimulatory effect on the adrenal cortex. Clarifying of the ghrelin precise role in the multiple networks affecting the stress hormone release, besides its well known energy and metabolic disbalance effects, remains a very important research goal.",
publisher = "Slovak Academy of Sciences",
journal = "General Physiology and Biophysics",
title = "Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study",
number = "2",
volume = "29",
doi = "10.4149/gpb_2010_02_194",
pages = "194-202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1371"
}

Milošević, V., Stevanović, D. M., Nešić, D. M., Šošić-Jurjević, B., Ajdžanović, V., Starčević, V. P.,& Severs, W. B.. (2010). Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study. in General Physiology and Biophysics
Slovak Academy of Sciences., 29(2), 194-202.
https://doi.org/10.4149/gpb_2010_02_194
https://hdl.handle.net/21.15107/rcub_ibiss_1371

Milošević V, Stevanović DM, Nešić DM, Šošić-Jurjević B, Ajdžanović V, Starčević VP, Severs WB. Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study. in General Physiology and Biophysics. 2010;29(2):194-202.
doi:10.4149/gpb_2010_02_194
https://hdl.handle.net/21.15107/rcub_ibiss_1371 .

Milošević, Verica, Stevanović, Darko M., Nešić, Dejan M., Šošić-Jurjević, Branka , Ajdžanović, Vladimir, Starčević, Vesna P., Severs, Walter B, "Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study" in General Physiology and Biophysics, 29, no. 2 (2010):194-202,
https://doi.org/10.4149/gpb_2010_02_194 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1371 .

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Nesić, Dejan M
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1535
AB  - Ghrelin, an endogenous ligand for the growth-hormone-secretagogue receptor, is a 28-amino acid peptide with a post-translational acyl modification necessary for its activity. It has central nervous system actions that affect appetite, body mass and energy balance. An intracerebroventricular (ICV) injection protocol of sub-nanomolar doses of ghrelin, known to alter the morphology of ACTH and GH producing pituicytes and plasma levels of these hormones, was used to provide an overview of metabolic changes linked to energy metabolism. Variables measured were: food intake (FI), water intake (WI), fecal mass, urine volume, body weight (BW), retroperitoneal (RP) and epididymal (EPI) white adipose tissue (WAT), and changes in serum leptin, insulin, triglycerides, cholesterol, and glucose. Five injections of rat ghrelin or PBS (n=8 per group) were given ICV every 24 h (1 mu g/5 mu L PBS) to adult male rats. Ghrelin had a positive and cumulative effect on FI, WI and BW (p<0.05), but not feces mass or urine volume (p>0.05). Centrally applied ghrelin clearly increased RP WAT (by 235%, p<0.001), EPI WAT (by 85%, p<0.05) and serum insulin levels (by 43%, p<0.05), and decreased serum leptin levels (by 77%, p<0.05) without (p>0.05) evoking changes in blood triglyceride cholesterol, or glucose levels. These data and the available literature clearly document that exposure of the brain of normal rats, over time, to sub-nanomolar doses of ghrelin results in metabolic dysregulation culminating in increased body mass, consummatory behavior, and lipid stores as well as changes in blood leptin/insulin levels. Thus, modulation of central ghrelin receptors may represent a pharmacological approach for controlling multiple factors involved in energy balance and obesity. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Regulatory Peptides
T1  - Consummatory behavior and metabolic indicators after central ghrelin injections in rats
IS  - 1-3
VL  - 147
EP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1535
ER  - 

@article{
author = "Stevanović, Darko M and Nesić, Dejan M and Milošević, Verica and Starčević, Vesna P. and Severs, Walter B",
year = "2008",
abstract = "Ghrelin, an endogenous ligand for the growth-hormone-secretagogue receptor, is a 28-amino acid peptide with a post-translational acyl modification necessary for its activity. It has central nervous system actions that affect appetite, body mass and energy balance. An intracerebroventricular (ICV) injection protocol of sub-nanomolar doses of ghrelin, known to alter the morphology of ACTH and GH producing pituicytes and plasma levels of these hormones, was used to provide an overview of metabolic changes linked to energy metabolism. Variables measured were: food intake (FI), water intake (WI), fecal mass, urine volume, body weight (BW), retroperitoneal (RP) and epididymal (EPI) white adipose tissue (WAT), and changes in serum leptin, insulin, triglycerides, cholesterol, and glucose. Five injections of rat ghrelin or PBS (n=8 per group) were given ICV every 24 h (1 mu g/5 mu L PBS) to adult male rats. Ghrelin had a positive and cumulative effect on FI, WI and BW (p<0.05), but not feces mass or urine volume (p>0.05). Centrally applied ghrelin clearly increased RP WAT (by 235%, p<0.001), EPI WAT (by 85%, p<0.05) and serum insulin levels (by 43%, p<0.05), and decreased serum leptin levels (by 77%, p<0.05) without (p>0.05) evoking changes in blood triglyceride cholesterol, or glucose levels. These data and the available literature clearly document that exposure of the brain of normal rats, over time, to sub-nanomolar doses of ghrelin results in metabolic dysregulation culminating in increased body mass, consummatory behavior, and lipid stores as well as changes in blood leptin/insulin levels. Thus, modulation of central ghrelin receptors may represent a pharmacological approach for controlling multiple factors involved in energy balance and obesity. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Regulatory Peptides",
title = "Consummatory behavior and metabolic indicators after central ghrelin injections in rats",
number = "1-3",
volume = "147",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1535"
}

Stevanović, D. M., Nesić, D. M., Milošević, V., Starčević, V. P.,& Severs, W. B.. (2008). Consummatory behavior and metabolic indicators after central ghrelin injections in rats. in Regulatory Peptides, 147(1-3).
https://hdl.handle.net/21.15107/rcub_ibiss_1535

Stevanović DM, Nesić DM, Milošević V, Starčević VP, Severs WB. Consummatory behavior and metabolic indicators after central ghrelin injections in rats. in Regulatory Peptides. 2008;147(1-3):null-59.
https://hdl.handle.net/21.15107/rcub_ibiss_1535 .

Stevanović, Darko M, Nesić, Dejan M, Milošević, Verica, Starčević, Vesna P., Severs, Walter B, "Consummatory behavior and metabolic indicators after central ghrelin injections in rats" in Regulatory Peptides, 147, no. 1-3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1535 .

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Milošević, Verica
AU  - Nesić, Dejan M
AU  - Ajdžanović, Vladimir
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1629
AB  - Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally purified from rat stomach; subsequently, ghrelin neurons were found in the arcuate nuclei of rats. Central effects of the peptide on GH release, however, remain to be clarified. The aim of the present study was to determine the morphologic features of GH-producing pituicytes and serum GH concentration after central administration of ghrelin. Five injections of rat ghrelin or phosphate-buffered saline (PBS; n = 10 rats/group) were given every 24 hrs (1 mu g of ghrelin in 5 mu l of PBS) into the lateral cerebral ventricle of male rats. Significant (P < 0.05) increases in absolute and relative pituitary weights occurred in ghrelin-treated rats versus controls (58% and 41%, respectively). Morphometric parameters (i.e., the volume of GH cells, volume of their nuclei, and volume density) all significantly (P < 0.05) increased by 17%, 18%, and 19%, respectively, in the ghrelin-treated group versus controls. Terminal serum concentration of GH was significantly (P < 0.05) increased by 15% with ghrelin treatment. The results clearly document that daily nanomolar doses of ghrelin into the lateral cerebral ventricle stimulate GH cell proliferation and promote GH release. Thus, achieving pharmacologic control of central ghrelin receptors is a promising modality to modulate the actions of GH.
T2  - Experimental Biology and Medicine
T1  - Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats
IS  - 10
VL  - 231
EP  - 1615
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1629
ER  - 

@article{
author = "Stevanović, Darko M and Milošević, Verica and Nesić, Dejan M and Ajdžanović, Vladimir and Starčević, Vesna P. and Severs, Walter B",
year = "2006",
abstract = "Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally purified from rat stomach; subsequently, ghrelin neurons were found in the arcuate nuclei of rats. Central effects of the peptide on GH release, however, remain to be clarified. The aim of the present study was to determine the morphologic features of GH-producing pituicytes and serum GH concentration after central administration of ghrelin. Five injections of rat ghrelin or phosphate-buffered saline (PBS; n = 10 rats/group) were given every 24 hrs (1 mu g of ghrelin in 5 mu l of PBS) into the lateral cerebral ventricle of male rats. Significant (P < 0.05) increases in absolute and relative pituitary weights occurred in ghrelin-treated rats versus controls (58% and 41%, respectively). Morphometric parameters (i.e., the volume of GH cells, volume of their nuclei, and volume density) all significantly (P < 0.05) increased by 17%, 18%, and 19%, respectively, in the ghrelin-treated group versus controls. Terminal serum concentration of GH was significantly (P < 0.05) increased by 15% with ghrelin treatment. The results clearly document that daily nanomolar doses of ghrelin into the lateral cerebral ventricle stimulate GH cell proliferation and promote GH release. Thus, achieving pharmacologic control of central ghrelin receptors is a promising modality to modulate the actions of GH.",
journal = "Experimental Biology and Medicine",
title = "Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats",
number = "10",
volume = "231",
pages = "1615",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1629"
}

Stevanović, D. M., Milošević, V., Nesić, D. M., Ajdžanović, V., Starčević, V. P.,& Severs, W. B.. (2006). Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats. in Experimental Biology and Medicine, 231(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1629

Stevanović DM, Milošević V, Nesić DM, Ajdžanović V, Starčević VP, Severs WB. Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats. in Experimental Biology and Medicine. 2006;231(10):null-1615.
https://hdl.handle.net/21.15107/rcub_ibiss_1629 .

Stevanović, Darko M, Milošević, Verica, Nesić, Dejan M, Ajdžanović, Vladimir, Starčević, Vesna P., Severs, Walter B, "Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats" in Experimental Biology and Medicine, 231, no. 10 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1629 .