Stevanović, Darko M

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  • Stevanović, Darko M (12)
  • Stevanović, Darko M. (1)
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Author's Bibliography

Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats

Nesić, Dejan M; Stevanović, Darko M; Stanković, Sanja Đ; Milošević, Verica; Trajković, Vladimir S; Starčević, Vesna P.; Severs, Walter B

(2013)

TY  - JOUR
AU  - Nesić, Dejan M
AU  - Stevanović, Darko M
AU  - Stanković, Sanja Đ
AU  - Milošević, Verica
AU  - Trajković, Vladimir S
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/997
AB  - Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related metabolic disturbances. We evaluated the influence of age on the orexigenic and lipid metabolism-altering effects of ghrelin. Peripubertal, young, adult and middle-aged rats (1, 2, 7 and 12 months old, respectively) were treated with 5 daily intracerebroventricular injections of ghrelin (0.15 nmol) or saline (control). The food intake was measured daily before treatment, while white adipose tissue and serum/plasma samples for detection of lipid metabolites/hormones were collected at the end of the experiment. The values of cumulative food intake and body weight gain declined, while the white adipose tissue deposits and blood concentrations of triglycerides, cholesterol and free fatty acids all increased with age. Ghrelin significantly increased all parameters, but the stimulatory effects on body weight gain and food intake were more pronounced in peripubertal/young rats, while the increase in white adipose mass, triglyceride and low-density lipoprotein cholesterol levels was more noticeable in adult/middle-aged animals. The decrease in sensitivity to ghrelin-mediated stimulation of food intake in older animals could not be explained by alterations in ghrelin's ability to reduce anorexigenic hormones insulin and leptin. However, the higher responsiveness of aged rats to ghrelin-mediated increase in lipid metabolites was accompanied by an increase in adrenocorticotropic hormone and corticosterone levels. These results indicate that aging, while reducing sensitivity to ghrelin-mediated increase in body weight gain and food intake, might enhance the responsiveness to the stimulatory effects of ghrelin on lipid metabolites and hypothalamic-pituitary-adrenal axis activity. (C) 2013 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmacology
T1  - Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats
IS  - 1-3
VL  - 710
SP  - 153
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_997
ER  - 
@article{
author = "Nesić, Dejan M and Stevanović, Darko M and Stanković, Sanja Đ and Milošević, Verica and Trajković, Vladimir S and Starčević, Vesna P. and Severs, Walter B",
year = "2013",
abstract = "Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related metabolic disturbances. We evaluated the influence of age on the orexigenic and lipid metabolism-altering effects of ghrelin. Peripubertal, young, adult and middle-aged rats (1, 2, 7 and 12 months old, respectively) were treated with 5 daily intracerebroventricular injections of ghrelin (0.15 nmol) or saline (control). The food intake was measured daily before treatment, while white adipose tissue and serum/plasma samples for detection of lipid metabolites/hormones were collected at the end of the experiment. The values of cumulative food intake and body weight gain declined, while the white adipose tissue deposits and blood concentrations of triglycerides, cholesterol and free fatty acids all increased with age. Ghrelin significantly increased all parameters, but the stimulatory effects on body weight gain and food intake were more pronounced in peripubertal/young rats, while the increase in white adipose mass, triglyceride and low-density lipoprotein cholesterol levels was more noticeable in adult/middle-aged animals. The decrease in sensitivity to ghrelin-mediated stimulation of food intake in older animals could not be explained by alterations in ghrelin's ability to reduce anorexigenic hormones insulin and leptin. However, the higher responsiveness of aged rats to ghrelin-mediated increase in lipid metabolites was accompanied by an increase in adrenocorticotropic hormone and corticosterone levels. These results indicate that aging, while reducing sensitivity to ghrelin-mediated increase in body weight gain and food intake, might enhance the responsiveness to the stimulatory effects of ghrelin on lipid metabolites and hypothalamic-pituitary-adrenal axis activity. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmacology",
title = "Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats",
number = "1-3",
volume = "710",
pages = "153-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_997"
}
Nesić, D. M., Stevanović, D. M., Stanković, S. Đ., Milošević, V., Trajković, V. S., Starčević, V. P.,& Severs, W. B.. (2013). Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats. in European Journal of Pharmacology, 710(1-3), 153-91.
https://hdl.handle.net/21.15107/rcub_ibiss_997
Nesić DM, Stevanović DM, Stanković SĐ, Milošević V, Trajković VS, Starčević VP, Severs WB. Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats. in European Journal of Pharmacology. 2013;710(1-3):153-91.
https://hdl.handle.net/21.15107/rcub_ibiss_997 .
Nesić, Dejan M, Stevanović, Darko M, Stanković, Sanja Đ, Milošević, Verica, Trajković, Vladimir S, Starčević, Vesna P., Severs, Walter B, "Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats" in European Journal of Pharmacology, 710, no. 1-3 (2013):153-91,
https://hdl.handle.net/21.15107/rcub_ibiss_997 .

Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study

Milošević, Verica; Ajdžanović, Vladimir; Filipović, Branko; Nesić, Dejan M; Starčević, Vesna P.; Rakocević, Rastko; Stevanović, Darko M

(2013)

TY  - JOUR
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Nesić, Dejan M
AU  - Starčević, Vesna P.
AU  - Rakocević, Rastko
AU  - Stevanović, Darko M
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1048
AB  - Changes in feeding regime represent serious stress, while ghrelin is considered a key player in energy balance. We investigated the effects of intracerebroventricular (ICV) ghrelin application on pituitary adrenocorticotropic (ACTH) cells in rats fed diets differing in energy content. Before the ICV treatment, male Wistar rats were subjected to three different feeding regimes for 4 weeks: normal-fed (NF), food-restricted (FR) or high-fed (HF) (n = 3 x 14). At the age of 8 weeks, rats from each group were divided into two subgroups and given ICV, either ghrelin (G; 1 mu g ghrelin/5 mu l PBS, n = 7) or solvent alone (5 mu l PBS, n = 7) every 24 h for 5 days. The immunohistochemical appearance and quantitative morphology of pituitary ACTH cells were evaluated, as well as peripheral ACTH and corticosterone levels. Central ghrelin administration increased (p < 0.05) ACTH cell volumes in GNF, GFR and GHF rats by 8.1%, 11.8% and 9.1%, respectively, compared to the controls, while significant increases in ACTH cell volume density were observed in GNF and GHF rats. Circulating ACTH and corticosterone levels were elevated (p < 0.05) in GNF and GFR rats by 72.8% and 80.8%, respectively, when compared to the corresponding controls. Thus, central ghrelin administration stimulated the pituitary-adrenal axis under preserved and negative energy balance states. (C) 2013 Elsevier GmbH. All rights reserved.
T2  - Acta Histochemica
T1  - Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study
IS  - 8
VL  - 115
SP  - 283
EP  - 864
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1048
ER  - 
@article{
author = "Milošević, Verica and Ajdžanović, Vladimir and Filipović, Branko and Nesić, Dejan M and Starčević, Vesna P. and Rakocević, Rastko and Stevanović, Darko M",
year = "2013",
abstract = "Changes in feeding regime represent serious stress, while ghrelin is considered a key player in energy balance. We investigated the effects of intracerebroventricular (ICV) ghrelin application on pituitary adrenocorticotropic (ACTH) cells in rats fed diets differing in energy content. Before the ICV treatment, male Wistar rats were subjected to three different feeding regimes for 4 weeks: normal-fed (NF), food-restricted (FR) or high-fed (HF) (n = 3 x 14). At the age of 8 weeks, rats from each group were divided into two subgroups and given ICV, either ghrelin (G; 1 mu g ghrelin/5 mu l PBS, n = 7) or solvent alone (5 mu l PBS, n = 7) every 24 h for 5 days. The immunohistochemical appearance and quantitative morphology of pituitary ACTH cells were evaluated, as well as peripheral ACTH and corticosterone levels. Central ghrelin administration increased (p < 0.05) ACTH cell volumes in GNF, GFR and GHF rats by 8.1%, 11.8% and 9.1%, respectively, compared to the controls, while significant increases in ACTH cell volume density were observed in GNF and GHF rats. Circulating ACTH and corticosterone levels were elevated (p < 0.05) in GNF and GFR rats by 72.8% and 80.8%, respectively, when compared to the corresponding controls. Thus, central ghrelin administration stimulated the pituitary-adrenal axis under preserved and negative energy balance states. (C) 2013 Elsevier GmbH. All rights reserved.",
journal = "Acta Histochemica",
title = "Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study",
number = "8",
volume = "115",
pages = "283-864",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1048"
}
Milošević, V., Ajdžanović, V., Filipović, B., Nesić, D. M., Starčević, V. P., Rakocević, R.,& Stevanović, D. M.. (2013). Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study. in Acta Histochemica, 115(8), 283-864.
https://hdl.handle.net/21.15107/rcub_ibiss_1048
Milošević V, Ajdžanović V, Filipović B, Nesić DM, Starčević VP, Rakocević R, Stevanović DM. Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study. in Acta Histochemica. 2013;115(8):283-864.
https://hdl.handle.net/21.15107/rcub_ibiss_1048 .
Milošević, Verica, Ajdžanović, Vladimir, Filipović, Branko, Nesić, Dejan M, Starčević, Vesna P., Rakocević, Rastko, Stevanović, Darko M, "Central ghrelin treatment stimulates ACTH cells in normal-fed, food-restricted and high-fed rats: An immunohistomorphometric and hormonal study" in Acta Histochemica, 115, no. 8 (2013):283-864,
https://hdl.handle.net/21.15107/rcub_ibiss_1048 .

Immunomodulatory actions of central ghrelin in diet-induced energy imbalance

Stevanović, Darko M; Starčević, Vesna P.; Vilimanović, Uros; Nesić, Dejan M; Vučićević, Ljubica; Misirkić Marjanović, Maja; Janjetović, Kristina; Savić, Emina; Popadić, Dusan M; Sudar, Emina M; Micić, Dragan D; Sumarac-Dumanović, Mirjana S; Trajković, Vladimir S

(2012)

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Starčević, Vesna P.
AU  - Vilimanović, Uros
AU  - Nesić, Dejan M
AU  - Vučićević, Ljubica
AU  - Misirkić Marjanović, Maja
AU  - Janjetović, Kristina
AU  - Savić, Emina
AU  - Popadić, Dusan M
AU  - Sudar, Emina M
AU  - Micić, Dragan D
AU  - Sumarac-Dumanović, Mirjana S
AU  - Trajković, Vladimir S
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1238
AB  - We investigated the effects of centrally administered orexigenic hormone ghrelin on energy imbalance-induced inflammation. Rats were subjected for four weeks to three different dietary regimes: normal (standard food), high-fat (standard food with 30% lard) or food-restricted (70%, 50%, 40% and 40% of the expected food intake in 1st, 2nd, 3rd and 4th week, respectively). Compared to normal-weight controls, starved, but not obese rats had significantly higher levels of proinflammatory cytokines (TNF, IL-1 beta, IFN-gamma) in the blood. When compared to normally fed animals, the hearts of starved and obese animals expressed higher levels of mRNAs encoding proinflammatory mediators (TNF, IL-1 beta, IL-6, IFN-gamma, IL-17, IL-12, iNOS), while mRNA levels of the anti-inflammatory TGF-beta remained unchanged. Intracerebroventricular (ICV) injection of ghrelin (1 mu g/day) for five consecutive days significantly reduced TNF, IL-1 beta and IFN-gamma levels in the blood of starved rats, as well as TNF, IL-17 and IL-12p40 mRNA expression in the hearts of obese rats. Conversely, ICV ghrelin increased the levels of 1FN-gamma, IL-17,1L-12p35 and IL-12p40 mRNA in the heart tissue of food-restricted animals. This was associated with an increase of immunosuppressive ACTH/corticosterone production in starved animals and a decrease of the immunostimulatory adipokine leptin both in food-restricted and high-fat groups. Ghrelin activated the energy sensor AMP-activated protein kinase (AMPK) in the hypothalamus and inhibited extracellular signal-regulated kinase (ERK) in the hearts of obese, but not starved rats. Therefore, central ghrelin may play a complex role in energy imbalance-induced inflammation by modulating HPA axis, leptin and AMPK/ERK signaling pathways. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Brain Behavior and Immunity
T1  - Immunomodulatory actions of central ghrelin in diet-induced energy imbalance
IS  - 1
VL  - 26
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1238
ER  - 
@article{
author = "Stevanović, Darko M and Starčević, Vesna P. and Vilimanović, Uros and Nesić, Dejan M and Vučićević, Ljubica and Misirkić Marjanović, Maja and Janjetović, Kristina and Savić, Emina and Popadić, Dusan M and Sudar, Emina M and Micić, Dragan D and Sumarac-Dumanović, Mirjana S and Trajković, Vladimir S",
year = "2012",
abstract = "We investigated the effects of centrally administered orexigenic hormone ghrelin on energy imbalance-induced inflammation. Rats were subjected for four weeks to three different dietary regimes: normal (standard food), high-fat (standard food with 30% lard) or food-restricted (70%, 50%, 40% and 40% of the expected food intake in 1st, 2nd, 3rd and 4th week, respectively). Compared to normal-weight controls, starved, but not obese rats had significantly higher levels of proinflammatory cytokines (TNF, IL-1 beta, IFN-gamma) in the blood. When compared to normally fed animals, the hearts of starved and obese animals expressed higher levels of mRNAs encoding proinflammatory mediators (TNF, IL-1 beta, IL-6, IFN-gamma, IL-17, IL-12, iNOS), while mRNA levels of the anti-inflammatory TGF-beta remained unchanged. Intracerebroventricular (ICV) injection of ghrelin (1 mu g/day) for five consecutive days significantly reduced TNF, IL-1 beta and IFN-gamma levels in the blood of starved rats, as well as TNF, IL-17 and IL-12p40 mRNA expression in the hearts of obese rats. Conversely, ICV ghrelin increased the levels of 1FN-gamma, IL-17,1L-12p35 and IL-12p40 mRNA in the heart tissue of food-restricted animals. This was associated with an increase of immunosuppressive ACTH/corticosterone production in starved animals and a decrease of the immunostimulatory adipokine leptin both in food-restricted and high-fat groups. Ghrelin activated the energy sensor AMP-activated protein kinase (AMPK) in the hypothalamus and inhibited extracellular signal-regulated kinase (ERK) in the hearts of obese, but not starved rats. Therefore, central ghrelin may play a complex role in energy imbalance-induced inflammation by modulating HPA axis, leptin and AMPK/ERK signaling pathways. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Brain Behavior and Immunity",
title = "Immunomodulatory actions of central ghrelin in diet-induced energy imbalance",
number = "1",
volume = "26",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1238"
}
Stevanović, D. M., Starčević, V. P., Vilimanović, U., Nesić, D. M., Vučićević, L., Misirkić Marjanović, M., Janjetović, K., Savić, E., Popadić, D. M., Sudar, E. M., Micić, D. D., Sumarac-Dumanović, M. S.,& Trajković, V. S.. (2012). Immunomodulatory actions of central ghrelin in diet-induced energy imbalance. in Brain Behavior and Immunity, 26(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1238
Stevanović DM, Starčević VP, Vilimanović U, Nesić DM, Vučićević L, Misirkić Marjanović M, Janjetović K, Savić E, Popadić DM, Sudar EM, Micić DD, Sumarac-Dumanović MS, Trajković VS. Immunomodulatory actions of central ghrelin in diet-induced energy imbalance. in Brain Behavior and Immunity. 2012;26(1):null-158.
https://hdl.handle.net/21.15107/rcub_ibiss_1238 .
Stevanović, Darko M, Starčević, Vesna P., Vilimanović, Uros, Nesić, Dejan M, Vučićević, Ljubica, Misirkić Marjanović, Maja, Janjetović, Kristina, Savić, Emina, Popadić, Dusan M, Sudar, Emina M, Micić, Dragan D, Sumarac-Dumanović, Mirjana S, Trajković, Vladimir S, "Immunomodulatory actions of central ghrelin in diet-induced energy imbalance" in Brain Behavior and Immunity, 26, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1238 .

Intracerebroventricular Administration of Metformin Inhibits Ghrelin-Induced Hypothalamic AMP-Kinase Signalling and Food Intake

Stevanović, Darko M; Janjetović, Kristina; Misirkić Marjanović, Maja; Vučićević, Ljubica; Sumarac-Dumanović, Mirjana S; Micić, Dragan D; Starčević, Vesna P.; Trajković, Vladimir S

(2012)

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Janjetović, Kristina
AU  - Misirkić Marjanović, Maja
AU  - Vučićević, Ljubica
AU  - Sumarac-Dumanović, Mirjana S
AU  - Micić, Dragan D
AU  - Starčević, Vesna P.
AU  - Trajković, Vladimir S
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1226
AB  - Background/Aims: The antihyperglycaemic drug metformin reduces food consumption through mechanisms that are not fully elucidated. The present study investigated the effects of intracerebroventricular administration of metformin on food intake and hypothalamic appetite-regulating signalling pathways induced by the orexigenic peptide ghrelin. Methods: Rats were injected intracerebroventricularly with ghrelin (5 mu g), metformin (50, 100 or 200 mu g), 5-amino-imidazole-4-carboxamide 1-beta-D-ribofuranoside (AICAR, 25 mu g) and L-Ieucine (1 mu g) in different combinations. Food intake was monitored during the next 4 h. Hypothalamic activation of AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), regulatory-associated protein of mTOR (Raptor), mammalian target of rapamycin (mTOR) and p70 S6 kinase 1 (S6K) after 1 h of treatment was analysed by immunoblotting. Results: Metformin suppressed the increase in food consumption induced by intracerebroventricular ghrelin in a dose-dependent manner. Ghrelin increased phosphorylation of hypothalamic AMPK and its targets ACC and Raptor, which was associated with the reduced phosphorylation of mTOR. The mTOR substrate, 56K, was activated by intracerebroventricular ghrelin despite the inhibition of mTOR. Metformin treatment blocked ghrelin-induced activation of hypothalamic AMPK/ACC/Raptor and restored mTOR activity without affecting 56K phosphorylation. Metformin also reduced food consumption induced by the AMPK activator AICAR while the ghrelin-triggered food intake was inhibited by the mTOR activator L-leucine. Conclusion: Metformin could reduce food intake by preventing ghrelin-induced AMPK signalling and mTOR inhibition in the hypotalamus. Copyright (c) 2012 S. Karger AG, Basel
T2  - Neuroendocrinology
T1  - Intracerebroventricular Administration of Metformin Inhibits Ghrelin-Induced Hypothalamic AMP-Kinase Signalling and Food Intake
IS  - 1
VL  - 96
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1226
ER  - 
@article{
author = "Stevanović, Darko M and Janjetović, Kristina and Misirkić Marjanović, Maja and Vučićević, Ljubica and Sumarac-Dumanović, Mirjana S and Micić, Dragan D and Starčević, Vesna P. and Trajković, Vladimir S",
year = "2012",
abstract = "Background/Aims: The antihyperglycaemic drug metformin reduces food consumption through mechanisms that are not fully elucidated. The present study investigated the effects of intracerebroventricular administration of metformin on food intake and hypothalamic appetite-regulating signalling pathways induced by the orexigenic peptide ghrelin. Methods: Rats were injected intracerebroventricularly with ghrelin (5 mu g), metformin (50, 100 or 200 mu g), 5-amino-imidazole-4-carboxamide 1-beta-D-ribofuranoside (AICAR, 25 mu g) and L-Ieucine (1 mu g) in different combinations. Food intake was monitored during the next 4 h. Hypothalamic activation of AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), regulatory-associated protein of mTOR (Raptor), mammalian target of rapamycin (mTOR) and p70 S6 kinase 1 (S6K) after 1 h of treatment was analysed by immunoblotting. Results: Metformin suppressed the increase in food consumption induced by intracerebroventricular ghrelin in a dose-dependent manner. Ghrelin increased phosphorylation of hypothalamic AMPK and its targets ACC and Raptor, which was associated with the reduced phosphorylation of mTOR. The mTOR substrate, 56K, was activated by intracerebroventricular ghrelin despite the inhibition of mTOR. Metformin treatment blocked ghrelin-induced activation of hypothalamic AMPK/ACC/Raptor and restored mTOR activity without affecting 56K phosphorylation. Metformin also reduced food consumption induced by the AMPK activator AICAR while the ghrelin-triggered food intake was inhibited by the mTOR activator L-leucine. Conclusion: Metformin could reduce food intake by preventing ghrelin-induced AMPK signalling and mTOR inhibition in the hypotalamus. Copyright (c) 2012 S. Karger AG, Basel",
journal = "Neuroendocrinology",
title = "Intracerebroventricular Administration of Metformin Inhibits Ghrelin-Induced Hypothalamic AMP-Kinase Signalling and Food Intake",
number = "1",
volume = "96",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1226"
}
Stevanović, D. M., Janjetović, K., Misirkić Marjanović, M., Vučićević, L., Sumarac-Dumanović, M. S., Micić, D. D., Starčević, V. P.,& Trajković, V. S.. (2012). Intracerebroventricular Administration of Metformin Inhibits Ghrelin-Induced Hypothalamic AMP-Kinase Signalling and Food Intake. in Neuroendocrinology, 96(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1226
Stevanović DM, Janjetović K, Misirkić Marjanović M, Vučićević L, Sumarac-Dumanović MS, Micić DD, Starčević VP, Trajković VS. Intracerebroventricular Administration of Metformin Inhibits Ghrelin-Induced Hypothalamic AMP-Kinase Signalling and Food Intake. in Neuroendocrinology. 2012;96(1):null-31.
https://hdl.handle.net/21.15107/rcub_ibiss_1226 .
Stevanović, Darko M, Janjetović, Kristina, Misirkić Marjanović, Maja, Vučićević, Ljubica, Sumarac-Dumanović, Mirjana S, Micić, Dragan D, Starčević, Vesna P., Trajković, Vladimir S, "Intracerebroventricular Administration of Metformin Inhibits Ghrelin-Induced Hypothalamic AMP-Kinase Signalling and Food Intake" in Neuroendocrinology, 96, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1226 .

Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment

Plecas-Solarović, Bosiljka A; Nesić, Dejan M; Stevanović, Darko M; Obradović, Aleksandar Lj; Đelić, Marina N; Milošević, Verica; Starčević, Vesna P.

(2012)

TY  - JOUR
AU  - Plecas-Solarović, Bosiljka A
AU  - Nesić, Dejan M
AU  - Stevanović, Darko M
AU  - Obradović, Aleksandar Lj
AU  - Đelić, Marina N
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1176
AB  - Ghrelin, the endogenous ligand of growth hormone secretagogue receptor type la (GHS-R1a), has emerged as pleiotropic modulator of diverse biological functions, including energy homeostasis and recently, reproduction. The influence of intracerebroventricularly (ICV) administered ghrelin (1 mu g/day/rat for 5 days) to rats of different ages, i.e., peripubertal (38 days), adult (60 days) and middle-aged (180 days) on the ventral prostate size and morphology, serum testosterone levels and testis weight was examined. Ghrelin treatment significantly increased (p < 0.05) absolute ventral prostate weight in peripubertal and middle-aged rats, by 27% and 37% respectively, due to enhancement of epithelial and/or luminal compartment of the gland. In adult rats, both absolute and relative volumes of the acinar lumen were significantly decreased (p < 0.05), by 38% and 44% respectively, which was associated with significant increases (p < 0.05) in relative and absolute volumes of interacinar stroma, whereas ventral prostate weigh was unchanged. Irrespective of animal age, ghrelin did not affect serum testosterone levels. These are the first results of ghrelin treatment effects on healthy prostate appearance, which allow us to conclude that the rat ventral prostate response to ghrelin depends on the developmental stage of animals. Our results merit further investigations and may have clinical implications, especially in the light of data on possible role of ghrelin in prostate hypertrophy and adenomas.
T2  - General Physiology and Biophysics
T1  - Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment
IS  - 2
VL  - 31
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1176
ER  - 
@article{
author = "Plecas-Solarović, Bosiljka A and Nesić, Dejan M and Stevanović, Darko M and Obradović, Aleksandar Lj and Đelić, Marina N and Milošević, Verica and Starčević, Vesna P.",
year = "2012",
abstract = "Ghrelin, the endogenous ligand of growth hormone secretagogue receptor type la (GHS-R1a), has emerged as pleiotropic modulator of diverse biological functions, including energy homeostasis and recently, reproduction. The influence of intracerebroventricularly (ICV) administered ghrelin (1 mu g/day/rat for 5 days) to rats of different ages, i.e., peripubertal (38 days), adult (60 days) and middle-aged (180 days) on the ventral prostate size and morphology, serum testosterone levels and testis weight was examined. Ghrelin treatment significantly increased (p < 0.05) absolute ventral prostate weight in peripubertal and middle-aged rats, by 27% and 37% respectively, due to enhancement of epithelial and/or luminal compartment of the gland. In adult rats, both absolute and relative volumes of the acinar lumen were significantly decreased (p < 0.05), by 38% and 44% respectively, which was associated with significant increases (p < 0.05) in relative and absolute volumes of interacinar stroma, whereas ventral prostate weigh was unchanged. Irrespective of animal age, ghrelin did not affect serum testosterone levels. These are the first results of ghrelin treatment effects on healthy prostate appearance, which allow us to conclude that the rat ventral prostate response to ghrelin depends on the developmental stage of animals. Our results merit further investigations and may have clinical implications, especially in the light of data on possible role of ghrelin in prostate hypertrophy and adenomas.",
journal = "General Physiology and Biophysics",
title = "Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment",
number = "2",
volume = "31",
pages = "210",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1176"
}
Plecas-Solarović, B. A., Nesić, D. M., Stevanović, D. M., Obradović, A. L., Đelić, M. N., Milošević, V.,& Starčević, V. P.. (2012). Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment. in General Physiology and Biophysics, 31(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1176
Plecas-Solarović BA, Nesić DM, Stevanović DM, Obradović AL, Đelić MN, Milošević V, Starčević VP. Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment. in General Physiology and Biophysics. 2012;31(2):null-210.
https://hdl.handle.net/21.15107/rcub_ibiss_1176 .
Plecas-Solarović, Bosiljka A, Nesić, Dejan M, Stevanović, Darko M, Obradović, Aleksandar Lj, Đelić, Marina N, Milošević, Verica, Starčević, Vesna P., "Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment" in General Physiology and Biophysics, 31, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1176 .

Adiposity Effects of Centrally Administered Ghrelin in Aged Rats

Brkić, Branislava; Nesić, Dejan M; Stevanović, Darko M; Đelić, Marina N; Milošević, Verica; Starčević, Vesna P.

(2011)

TY  - CONF
AU  - Brkić, Branislava
AU  - Nesić, Dejan M
AU  - Stevanović, Darko M
AU  - Đelić, Marina N
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1292
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Adiposity Effects of Centrally Administered Ghrelin in Aged Rats
IS  - null
VL  - 49
EP  - S631
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1292
ER  - 
@conference{
author = "Brkić, Branislava and Nesić, Dejan M and Stevanović, Darko M and Đelić, Marina N and Milošević, Verica and Starčević, Vesna P.",
year = "2011",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Adiposity Effects of Centrally Administered Ghrelin in Aged Rats",
number = "null",
volume = "49",
pages = "S631",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1292"
}
Brkić, B., Nesić, D. M., Stevanović, D. M., Đelić, M. N., Milošević, V.,& Starčević, V. P.. (2011). Adiposity Effects of Centrally Administered Ghrelin in Aged Rats. in Clinical Chemistry and Laboratory Medicine, 49(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1292
Brkić B, Nesić DM, Stevanović DM, Đelić MN, Milošević V, Starčević VP. Adiposity Effects of Centrally Administered Ghrelin in Aged Rats. in Clinical Chemistry and Laboratory Medicine. 2011;49(null):null-S631.
https://hdl.handle.net/21.15107/rcub_ibiss_1292 .
Brkić, Branislava, Nesić, Dejan M, Stevanović, Darko M, Đelić, Marina N, Milošević, Verica, Starčević, Vesna P., "Adiposity Effects of Centrally Administered Ghrelin in Aged Rats" in Clinical Chemistry and Laboratory Medicine, 49, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1292 .

Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study

Milošević, Verica; Stevanović, Darko M.; Nešić, Dejan M.; Šošić-Jurjević, Branka ; Ajdžanović, Vladimir; Starčević, Vesna P.; Severs, Walter B

(Slovak Academy of Sciences, 2010)

TY  - JOUR
AU  - Milošević, Verica
AU  - Stevanović, Darko M.
AU  - Nešić, Dejan M.
AU  - Šošić-Jurjević, Branka 
AU  - Ajdžanović, Vladimir
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1371
AB  - Ghrelin, a growth hormone secretagogue that exerts an important role in appetite and weight regulation, participates in the activation of the hypothalamo-pituitary-adrenal (HPA) axis. Male Wistar rats (5/group) received daily for 5 days, via an ICV (intracerebroventricular) cannula, 5 ill phosphate buffered saline with or without 1 mu g of rat ghrelin. Two hours after the last injection, blood and adrenal glands were collected from decapitated rats for blood hormone analyses and histologic and morphometric processing. Ghrelin treatment resulted in increased (p < 0.05) body weight (13%), absolute whole adrenal gland weight (18%) and whole adrenal gland volume (20%). The absolute volumes of the entire adrenal cortex, ZG, ZF, and ZR also increased (p < 0.05) after ghrelin by 20%, 21%, 21% and 11%, respectively. Ghrelin-treated rats had elevated (p < 0.05) blood concentrations of ACTH, aldosterone and corticosterone (68%, 32% and 67%, respectively). The data clearly provide both morphological and hormonal status that ghrelin acts centrally to exert a global stimulatory effect on the adrenal cortex. Clarifying of the ghrelin precise role in the multiple networks affecting the stress hormone release, besides its well known energy and metabolic disbalance effects, remains a very important research goal.
PB  - Slovak Academy of Sciences
T2  - General Physiology and Biophysics
T1  - Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study
IS  - 2
VL  - 29
DO  - 10.4149/gpb_2010_02_194
SP  - 194
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1371
ER  - 
@article{
author = "Milošević, Verica and Stevanović, Darko M. and Nešić, Dejan M. and Šošić-Jurjević, Branka  and Ajdžanović, Vladimir and Starčević, Vesna P. and Severs, Walter B",
year = "2010",
abstract = "Ghrelin, a growth hormone secretagogue that exerts an important role in appetite and weight regulation, participates in the activation of the hypothalamo-pituitary-adrenal (HPA) axis. Male Wistar rats (5/group) received daily for 5 days, via an ICV (intracerebroventricular) cannula, 5 ill phosphate buffered saline with or without 1 mu g of rat ghrelin. Two hours after the last injection, blood and adrenal glands were collected from decapitated rats for blood hormone analyses and histologic and morphometric processing. Ghrelin treatment resulted in increased (p < 0.05) body weight (13%), absolute whole adrenal gland weight (18%) and whole adrenal gland volume (20%). The absolute volumes of the entire adrenal cortex, ZG, ZF, and ZR also increased (p < 0.05) after ghrelin by 20%, 21%, 21% and 11%, respectively. Ghrelin-treated rats had elevated (p < 0.05) blood concentrations of ACTH, aldosterone and corticosterone (68%, 32% and 67%, respectively). The data clearly provide both morphological and hormonal status that ghrelin acts centrally to exert a global stimulatory effect on the adrenal cortex. Clarifying of the ghrelin precise role in the multiple networks affecting the stress hormone release, besides its well known energy and metabolic disbalance effects, remains a very important research goal.",
publisher = "Slovak Academy of Sciences",
journal = "General Physiology and Biophysics",
title = "Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study",
number = "2",
volume = "29",
doi = "10.4149/gpb_2010_02_194",
pages = "194-202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1371"
}
Milošević, V., Stevanović, D. M., Nešić, D. M., Šošić-Jurjević, B., Ajdžanović, V., Starčević, V. P.,& Severs, W. B.. (2010). Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study. in General Physiology and Biophysics
Slovak Academy of Sciences., 29(2), 194-202.
https://doi.org/10.4149/gpb_2010_02_194
https://hdl.handle.net/21.15107/rcub_ibiss_1371
Milošević V, Stevanović DM, Nešić DM, Šošić-Jurjević B, Ajdžanović V, Starčević VP, Severs WB. Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study. in General Physiology and Biophysics. 2010;29(2):194-202.
doi:10.4149/gpb_2010_02_194
https://hdl.handle.net/21.15107/rcub_ibiss_1371 .
Milošević, Verica, Stevanović, Darko M., Nešić, Dejan M., Šošić-Jurjević, Branka , Ajdžanović, Vladimir, Starčević, Vesna P., Severs, Walter B, "Central effects of ghrelin on the adrenal cortex: a morphological and hormonal study" in General Physiology and Biophysics, 29, no. 2 (2010):194-202,
https://doi.org/10.4149/gpb_2010_02_194 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1371 .
11
6
10

Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats

Šošić-Jurjević, Branka ; Stevanović, Darko M; Milošević, Verica; Sekulić, Milka I.; Starčević, Vesna P.

(Karger Publishers, 2009)

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Stevanović, Darko M
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
AU  - Starčević, Vesna P.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1486
AB  - Body weight depends on the balance between energy intake and consumption. An interaction between ghrelin and thyroid function has been reported only in pathophysiological states. We examined whether intracerebroventricular (ICV) administration of ghrelin affects the structure and function of the pituitary-thyroid axis in young adult male rats. Ghrelin (0.3 nmol/5 mu l PBS) or an equal volume of PBS were injected every 24 h into the lateral cerebral ventricle for 5 days. Two hours after the last treatment the animals were killed, their pituitaries and thyroids excised and prepared for further histological, immunohistochemical and morphometric investigation. Serum TSH levels were measured by RIA, while the total T(4) and T(3) levels were examined by ECLIA. Ghrelin treatment increased pituitary weight (p < 0.05) when compared to the controls, with no effect on the thyroid weight. Smaller, degranulated TSH-immunopositive cells were noticed within the pituitaries of ghrelin-treated animals; their cellular and nuclear volume as well as the relative volume density of thyrotrophs decreased (p < 0.05) in comparison to the control values. The level of serum TSH was reduced (p < 0.05). In the thyroid parenchyma of ghrelin-treated rats, an increased number of hypofunctioning follicles was noticed, characterized by flattened, weakly Tg-immunoreactive epithelium and colloid distension. The relative volume densities of the follicles and colloid increased (p < 0.05), while the thyroid index of activation rate and the serum level of total T(4) decreased (p < 0.05). In conclusion, centrally applied ghrelin modulated the immunohistomorphometric features of pituitary TSH cells and decreased the level of serum TSH, consequently changing thyroid morphology and function, by reducing the T(4) hormone level in the serum. Copyright (C) 2008 S. Karger AG, Basel
PB  - Karger Publishers
T2  - Neuroendocrinology
T1  - Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats
IS  - 3
VL  - 89
DO  - 10.1159/000188603
SP  - 327
EP  - 336
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1486
ER  - 
@article{
author = "Šošić-Jurjević, Branka  and Stevanović, Darko M and Milošević, Verica and Sekulić, Milka I. and Starčević, Vesna P.",
year = "2009",
abstract = "Body weight depends on the balance between energy intake and consumption. An interaction between ghrelin and thyroid function has been reported only in pathophysiological states. We examined whether intracerebroventricular (ICV) administration of ghrelin affects the structure and function of the pituitary-thyroid axis in young adult male rats. Ghrelin (0.3 nmol/5 mu l PBS) or an equal volume of PBS were injected every 24 h into the lateral cerebral ventricle for 5 days. Two hours after the last treatment the animals were killed, their pituitaries and thyroids excised and prepared for further histological, immunohistochemical and morphometric investigation. Serum TSH levels were measured by RIA, while the total T(4) and T(3) levels were examined by ECLIA. Ghrelin treatment increased pituitary weight (p < 0.05) when compared to the controls, with no effect on the thyroid weight. Smaller, degranulated TSH-immunopositive cells were noticed within the pituitaries of ghrelin-treated animals; their cellular and nuclear volume as well as the relative volume density of thyrotrophs decreased (p < 0.05) in comparison to the control values. The level of serum TSH was reduced (p < 0.05). In the thyroid parenchyma of ghrelin-treated rats, an increased number of hypofunctioning follicles was noticed, characterized by flattened, weakly Tg-immunoreactive epithelium and colloid distension. The relative volume densities of the follicles and colloid increased (p < 0.05), while the thyroid index of activation rate and the serum level of total T(4) decreased (p < 0.05). In conclusion, centrally applied ghrelin modulated the immunohistomorphometric features of pituitary TSH cells and decreased the level of serum TSH, consequently changing thyroid morphology and function, by reducing the T(4) hormone level in the serum. Copyright (C) 2008 S. Karger AG, Basel",
publisher = "Karger Publishers",
journal = "Neuroendocrinology",
title = "Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats",
number = "3",
volume = "89",
doi = "10.1159/000188603",
pages = "327-336",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1486"
}
Šošić-Jurjević, B., Stevanović, D. M., Milošević, V., Sekulić, M. I.,& Starčević, V. P.. (2009). Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats. in Neuroendocrinology
Karger Publishers., 89(3), 327-336.
https://doi.org/10.1159/000188603
https://hdl.handle.net/21.15107/rcub_ibiss_1486
Šošić-Jurjević B, Stevanović DM, Milošević V, Sekulić MI, Starčević VP. Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats. in Neuroendocrinology. 2009;89(3):327-336.
doi:10.1159/000188603
https://hdl.handle.net/21.15107/rcub_ibiss_1486 .
Šošić-Jurjević, Branka , Stevanović, Darko M, Milošević, Verica, Sekulić, Milka I., Starčević, Vesna P., "Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats" in Neuroendocrinology, 89, no. 3 (2009):327-336,
https://doi.org/10.1159/000188603 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1486 .
19
14
18

AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C

Vučićević, Ljubica; Misirkić Marjanović, Maja; Janjetović, Kristina; Harhaji-Trajković, Ljubica; Prica, Marko; Stevanović, Darko M; Isenović, Esma R; Sudar, Emina M; Sumarac-Dumanović, Mirjana S; Micić, Dragan D; Trajković, Vladimir S

(2009)

TY  - JOUR
AU  - Vučićević, Ljubica
AU  - Misirkić Marjanović, Maja
AU  - Janjetović, Kristina
AU  - Harhaji-Trajković, Ljubica
AU  - Prica, Marko
AU  - Stevanović, Darko M
AU  - Isenović, Esma R
AU  - Sudar, Emina M
AU  - Sumarac-Dumanović, Mirjana S
AU  - Micić, Dragan D
AU  - Trajković, Vladimir S
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1446
AB  - We investigated the effect of compound C, a well-known inhibitor of the intracellular energy sensor AMP-activated protein kinase (AMPK), on proliferation and viability of human U251 and rat C6 glioma cell lines. Compound C caused G(2)/M cell cycle block, accompanied by apoptotic glioma cell death characterized by caspase activation, phosphatidylserine exposure and DNA fragmentation. The mechanisms underlying the pro-apoptotic action of compound C involved induction of oxidative stress and downregulation of antiapoptotic molecule Bcl-2, while no alteration of pro-apoptotic Bax was observed. Compound C diminished AMPK phosphorylation and enzymatic activity, resulting in reduced phosphorylation of its target acetyl CoA carboxylase. AMPK activators metformin and AICAR partly prevented the cell cycle block, oxidative stress and apoptosis induced by compound C. The small interfering RNA (siRNA) targeting of human AMPK mimicked compound C-induced G(2)/M cell cycle arrest, but failed to induce oxidative stress and apoptosis in U251 glioma cells. In conclusion, our data indicate that AMPK inhibition is required, but not sufficient for compound C-mediated apoptotic death of glioma cells. (c) 2009 Elsevier Inc. All rights reserved.
T2  - Biochemical Pharmacology
T1  - AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C
IS  - 11
VL  - 77
EP  - 1693
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1446
ER  - 
@article{
author = "Vučićević, Ljubica and Misirkić Marjanović, Maja and Janjetović, Kristina and Harhaji-Trajković, Ljubica and Prica, Marko and Stevanović, Darko M and Isenović, Esma R and Sudar, Emina M and Sumarac-Dumanović, Mirjana S and Micić, Dragan D and Trajković, Vladimir S",
year = "2009",
abstract = "We investigated the effect of compound C, a well-known inhibitor of the intracellular energy sensor AMP-activated protein kinase (AMPK), on proliferation and viability of human U251 and rat C6 glioma cell lines. Compound C caused G(2)/M cell cycle block, accompanied by apoptotic glioma cell death characterized by caspase activation, phosphatidylserine exposure and DNA fragmentation. The mechanisms underlying the pro-apoptotic action of compound C involved induction of oxidative stress and downregulation of antiapoptotic molecule Bcl-2, while no alteration of pro-apoptotic Bax was observed. Compound C diminished AMPK phosphorylation and enzymatic activity, resulting in reduced phosphorylation of its target acetyl CoA carboxylase. AMPK activators metformin and AICAR partly prevented the cell cycle block, oxidative stress and apoptosis induced by compound C. The small interfering RNA (siRNA) targeting of human AMPK mimicked compound C-induced G(2)/M cell cycle arrest, but failed to induce oxidative stress and apoptosis in U251 glioma cells. In conclusion, our data indicate that AMPK inhibition is required, but not sufficient for compound C-mediated apoptotic death of glioma cells. (c) 2009 Elsevier Inc. All rights reserved.",
journal = "Biochemical Pharmacology",
title = "AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C",
number = "11",
volume = "77",
pages = "1693",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1446"
}
Vučićević, L., Misirkić Marjanović, M., Janjetović, K., Harhaji-Trajković, L., Prica, M., Stevanović, D. M., Isenović, E. R., Sudar, E. M., Sumarac-Dumanović, M. S., Micić, D. D.,& Trajković, V. S.. (2009). AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C. in Biochemical Pharmacology, 77(11).
https://hdl.handle.net/21.15107/rcub_ibiss_1446
Vučićević L, Misirkić Marjanović M, Janjetović K, Harhaji-Trajković L, Prica M, Stevanović DM, Isenović ER, Sudar EM, Sumarac-Dumanović MS, Micić DD, Trajković VS. AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C. in Biochemical Pharmacology. 2009;77(11):null-1693.
https://hdl.handle.net/21.15107/rcub_ibiss_1446 .
Vučićević, Ljubica, Misirkić Marjanović, Maja, Janjetović, Kristina, Harhaji-Trajković, Ljubica, Prica, Marko, Stevanović, Darko M, Isenović, Esma R, Sudar, Emina M, Sumarac-Dumanović, Mirjana S, Micić, Dragan D, Trajković, Vladimir S, "AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C" in Biochemical Pharmacology, 77, no. 11 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1446 .

Effects of ghrelin on the feeding behavior of Lymantria dispar L. (Lymantriidae) caterpillars

Perić Mataruga, Vesna; Mirčić, Dejan; Vlahović, Milena; Mrdaković, Marija; Todorović, Dajana; Stevanović, Darko M; Milošević, Verica

(Academic Press Ltd - Elsevier Science Ltd, 2009)

TY  - JOUR
AU  - Perić Mataruga, Vesna
AU  - Mirčić, Dejan
AU  - Vlahović, Milena
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Stevanović, Darko M
AU  - Milošević, Verica
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1437
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0195666309005194?via%3Dihub
AB  - Ghrelin is a 28-amino acid peptide which has significant effects on animal appetite, thus influencing body mass. The aim of our study was to examine the effects of ghrelin on the feeding behavior and physiology 4th instar caterpillars of the pest insect, Lymantria dispar L. Treatment of 4th instar caterpillars with four subpicomolar amounts of ghrelin had a positive influence on daily food intake, frass elimination, body mass. Also, locomotor activity increased, while stadium duration decreased in treated caterpillars. The similarity between the effects of ghrelin on caterpillar physiology and those in mammals suggests that using this model system for further studies of neuroendocrinological processes underlying feeding could lead to essential information about more complex organisms. (C) 2009 Elsevier Ltd. All rights reserved.
PB  - Academic Press Ltd - Elsevier Science Ltd
T2  - Appetite
T1  - Effects of ghrelin on the feeding behavior of Lymantria dispar L. (Lymantriidae) caterpillars
IS  - 1
VL  - 53
DO  - 10.1016/j.appet.2009.05.009
SP  - 147
EP  - 150
ER  - 
@article{
author = "Perić Mataruga, Vesna and Mirčić, Dejan and Vlahović, Milena and Mrdaković, Marija and Todorović, Dajana and Stevanović, Darko M and Milošević, Verica",
year = "2009",
abstract = "Ghrelin is a 28-amino acid peptide which has significant effects on animal appetite, thus influencing body mass. The aim of our study was to examine the effects of ghrelin on the feeding behavior and physiology 4th instar caterpillars of the pest insect, Lymantria dispar L. Treatment of 4th instar caterpillars with four subpicomolar amounts of ghrelin had a positive influence on daily food intake, frass elimination, body mass. Also, locomotor activity increased, while stadium duration decreased in treated caterpillars. The similarity between the effects of ghrelin on caterpillar physiology and those in mammals suggests that using this model system for further studies of neuroendocrinological processes underlying feeding could lead to essential information about more complex organisms. (C) 2009 Elsevier Ltd. All rights reserved.",
publisher = "Academic Press Ltd - Elsevier Science Ltd",
journal = "Appetite",
title = "Effects of ghrelin on the feeding behavior of Lymantria dispar L. (Lymantriidae) caterpillars",
number = "1",
volume = "53",
doi = "10.1016/j.appet.2009.05.009",
pages = "147-150"
}
Perić Mataruga, V., Mirčić, D., Vlahović, M., Mrdaković, M., Todorović, D., Stevanović, D. M.,& Milošević, V.. (2009). Effects of ghrelin on the feeding behavior of Lymantria dispar L. (Lymantriidae) caterpillars. in Appetite
Academic Press Ltd - Elsevier Science Ltd., 53(1), 147-150.
https://doi.org/10.1016/j.appet.2009.05.009
Perić Mataruga V, Mirčić D, Vlahović M, Mrdaković M, Todorović D, Stevanović DM, Milošević V. Effects of ghrelin on the feeding behavior of Lymantria dispar L. (Lymantriidae) caterpillars. in Appetite. 2009;53(1):147-150.
doi:10.1016/j.appet.2009.05.009 .
Perić Mataruga, Vesna, Mirčić, Dejan, Vlahović, Milena, Mrdaković, Marija, Todorović, Dajana, Stevanović, Darko M, Milošević, Verica, "Effects of ghrelin on the feeding behavior of Lymantria dispar L. (Lymantriidae) caterpillars" in Appetite, 53, no. 1 (2009):147-150,
https://doi.org/10.1016/j.appet.2009.05.009 . .
9
9
10

Consummatory behavior and metabolic indicators after central ghrelin injections in rats

Stevanović, Darko M; Nesić, Dejan M; Milošević, Verica; Starčević, Vesna P.; Severs, Walter B

(2008)

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Nesić, Dejan M
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1535
AB  - Ghrelin, an endogenous ligand for the growth-hormone-secretagogue receptor, is a 28-amino acid peptide with a post-translational acyl modification necessary for its activity. It has central nervous system actions that affect appetite, body mass and energy balance. An intracerebroventricular (ICV) injection protocol of sub-nanomolar doses of ghrelin, known to alter the morphology of ACTH and GH producing pituicytes and plasma levels of these hormones, was used to provide an overview of metabolic changes linked to energy metabolism. Variables measured were: food intake (FI), water intake (WI), fecal mass, urine volume, body weight (BW), retroperitoneal (RP) and epididymal (EPI) white adipose tissue (WAT), and changes in serum leptin, insulin, triglycerides, cholesterol, and glucose. Five injections of rat ghrelin or PBS (n=8 per group) were given ICV every 24 h (1 mu g/5 mu L PBS) to adult male rats. Ghrelin had a positive and cumulative effect on FI, WI and BW (p<0.05), but not feces mass or urine volume (p>0.05). Centrally applied ghrelin clearly increased RP WAT (by 235%, p<0.001), EPI WAT (by 85%, p<0.05) and serum insulin levels (by 43%, p<0.05), and decreased serum leptin levels (by 77%, p<0.05) without (p>0.05) evoking changes in blood triglyceride cholesterol, or glucose levels. These data and the available literature clearly document that exposure of the brain of normal rats, over time, to sub-nanomolar doses of ghrelin results in metabolic dysregulation culminating in increased body mass, consummatory behavior, and lipid stores as well as changes in blood leptin/insulin levels. Thus, modulation of central ghrelin receptors may represent a pharmacological approach for controlling multiple factors involved in energy balance and obesity. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Regulatory Peptides
T1  - Consummatory behavior and metabolic indicators after central ghrelin injections in rats
IS  - 1-3
VL  - 147
EP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1535
ER  - 
@article{
author = "Stevanović, Darko M and Nesić, Dejan M and Milošević, Verica and Starčević, Vesna P. and Severs, Walter B",
year = "2008",
abstract = "Ghrelin, an endogenous ligand for the growth-hormone-secretagogue receptor, is a 28-amino acid peptide with a post-translational acyl modification necessary for its activity. It has central nervous system actions that affect appetite, body mass and energy balance. An intracerebroventricular (ICV) injection protocol of sub-nanomolar doses of ghrelin, known to alter the morphology of ACTH and GH producing pituicytes and plasma levels of these hormones, was used to provide an overview of metabolic changes linked to energy metabolism. Variables measured were: food intake (FI), water intake (WI), fecal mass, urine volume, body weight (BW), retroperitoneal (RP) and epididymal (EPI) white adipose tissue (WAT), and changes in serum leptin, insulin, triglycerides, cholesterol, and glucose. Five injections of rat ghrelin or PBS (n=8 per group) were given ICV every 24 h (1 mu g/5 mu L PBS) to adult male rats. Ghrelin had a positive and cumulative effect on FI, WI and BW (p<0.05), but not feces mass or urine volume (p>0.05). Centrally applied ghrelin clearly increased RP WAT (by 235%, p<0.001), EPI WAT (by 85%, p<0.05) and serum insulin levels (by 43%, p<0.05), and decreased serum leptin levels (by 77%, p<0.05) without (p>0.05) evoking changes in blood triglyceride cholesterol, or glucose levels. These data and the available literature clearly document that exposure of the brain of normal rats, over time, to sub-nanomolar doses of ghrelin results in metabolic dysregulation culminating in increased body mass, consummatory behavior, and lipid stores as well as changes in blood leptin/insulin levels. Thus, modulation of central ghrelin receptors may represent a pharmacological approach for controlling multiple factors involved in energy balance and obesity. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Regulatory Peptides",
title = "Consummatory behavior and metabolic indicators after central ghrelin injections in rats",
number = "1-3",
volume = "147",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1535"
}
Stevanović, D. M., Nesić, D. M., Milošević, V., Starčević, V. P.,& Severs, W. B.. (2008). Consummatory behavior and metabolic indicators after central ghrelin injections in rats. in Regulatory Peptides, 147(1-3).
https://hdl.handle.net/21.15107/rcub_ibiss_1535
Stevanović DM, Nesić DM, Milošević V, Starčević VP, Severs WB. Consummatory behavior and metabolic indicators after central ghrelin injections in rats. in Regulatory Peptides. 2008;147(1-3):null-59.
https://hdl.handle.net/21.15107/rcub_ibiss_1535 .
Stevanović, Darko M, Nesić, Dejan M, Milošević, Verica, Starčević, Vesna P., Severs, Walter B, "Consummatory behavior and metabolic indicators after central ghrelin injections in rats" in Regulatory Peptides, 147, no. 1-3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1535 .

The effect of centrally administered ghrelin on pituitary ACTH cells and circulating ACTH and corticosterone in rats

Stevanović, Darko M; Milošević, Verica; Starčević, Vesna P.; Severs, Walter B

(2007)

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1610
AB  - Ghrelin is a brain-gut peptide known for its growth hormone (GH)-releasing and appetite-inducing activities. This natural GH secretagogue (GHS) was originally purified from rat stomach, but it is expressed widely in different tissues where it may have endocrine and paracrine effects. The central effects of ghrelin on adrenocorticotropic, hormone (ACTH) cells, ACTH release and subsequent corticosterone release from adrenal glands remains to be clarified. The aim of this study was to specifically determine the morphological features of ACTH-producing pituicytes and blood concentration of ACTH and corticosterone after central administration of ghrelin. Five doses of rat ghrelin or PBS (n = 10 per group) were injected every 24 h (1 mu g of ghrelin in 5 mu L PBS), into the lateral cerebral ventricle of male rats. Results showed that ghrelin increased (p < 0.05) absolute and relative pituitary weights compared to controls (58% and 41% respectively). Morphometric parameters, i.e. the volume of the ACTH cells, nuclear volume, and volume density were all increased (p < 0.05), by 17%, 6% and 13%, respectively, 2 h after the last ghrelin treatment. Ghrelin increased circulating concentrations of ACTH and corticosterone (p < 0.05) by 62% and 66%, respectively. The data provide clear documentation that intracerebroventricular ghrelin stimulates ACTH cell hypertrophy and proliferation, and promotes ACTH and corticosterone release. Determining the role of ghrelin in physiological stress responses and whether control of the peptide's activity would be useful for prevention and/or treatment of stress-induced diseases remain important research goals. (c) 2006 Elsevier Inc. All rights reserved.
T2  - Life Sciences
T1  - The effect of centrally administered ghrelin on pituitary ACTH cells and circulating ACTH and corticosterone in rats
IS  - 9
VL  - 80
EP  - 872
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1610
ER  - 
@article{
author = "Stevanović, Darko M and Milošević, Verica and Starčević, Vesna P. and Severs, Walter B",
year = "2007",
abstract = "Ghrelin is a brain-gut peptide known for its growth hormone (GH)-releasing and appetite-inducing activities. This natural GH secretagogue (GHS) was originally purified from rat stomach, but it is expressed widely in different tissues where it may have endocrine and paracrine effects. The central effects of ghrelin on adrenocorticotropic, hormone (ACTH) cells, ACTH release and subsequent corticosterone release from adrenal glands remains to be clarified. The aim of this study was to specifically determine the morphological features of ACTH-producing pituicytes and blood concentration of ACTH and corticosterone after central administration of ghrelin. Five doses of rat ghrelin or PBS (n = 10 per group) were injected every 24 h (1 mu g of ghrelin in 5 mu L PBS), into the lateral cerebral ventricle of male rats. Results showed that ghrelin increased (p < 0.05) absolute and relative pituitary weights compared to controls (58% and 41% respectively). Morphometric parameters, i.e. the volume of the ACTH cells, nuclear volume, and volume density were all increased (p < 0.05), by 17%, 6% and 13%, respectively, 2 h after the last ghrelin treatment. Ghrelin increased circulating concentrations of ACTH and corticosterone (p < 0.05) by 62% and 66%, respectively. The data provide clear documentation that intracerebroventricular ghrelin stimulates ACTH cell hypertrophy and proliferation, and promotes ACTH and corticosterone release. Determining the role of ghrelin in physiological stress responses and whether control of the peptide's activity would be useful for prevention and/or treatment of stress-induced diseases remain important research goals. (c) 2006 Elsevier Inc. All rights reserved.",
journal = "Life Sciences",
title = "The effect of centrally administered ghrelin on pituitary ACTH cells and circulating ACTH and corticosterone in rats",
number = "9",
volume = "80",
pages = "872",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1610"
}
Stevanović, D. M., Milošević, V., Starčević, V. P.,& Severs, W. B.. (2007). The effect of centrally administered ghrelin on pituitary ACTH cells and circulating ACTH and corticosterone in rats. in Life Sciences, 80(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1610
Stevanović DM, Milošević V, Starčević VP, Severs WB. The effect of centrally administered ghrelin on pituitary ACTH cells and circulating ACTH and corticosterone in rats. in Life Sciences. 2007;80(9):null-872.
https://hdl.handle.net/21.15107/rcub_ibiss_1610 .
Stevanović, Darko M, Milošević, Verica, Starčević, Vesna P., Severs, Walter B, "The effect of centrally administered ghrelin on pituitary ACTH cells and circulating ACTH and corticosterone in rats" in Life Sciences, 80, no. 9 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1610 .

Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats

Stevanović, Darko M; Milošević, Verica; Nesić, Dejan M; Ajdžanović, Vladimir; Starčević, Vesna P.; Severs, Walter B

(2006)

TY  - JOUR
AU  - Stevanović, Darko M
AU  - Milošević, Verica
AU  - Nesić, Dejan M
AU  - Ajdžanović, Vladimir
AU  - Starčević, Vesna P.
AU  - Severs, Walter B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1629
AB  - Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally purified from rat stomach; subsequently, ghrelin neurons were found in the arcuate nuclei of rats. Central effects of the peptide on GH release, however, remain to be clarified. The aim of the present study was to determine the morphologic features of GH-producing pituicytes and serum GH concentration after central administration of ghrelin. Five injections of rat ghrelin or phosphate-buffered saline (PBS; n = 10 rats/group) were given every 24 hrs (1 mu g of ghrelin in 5 mu l of PBS) into the lateral cerebral ventricle of male rats. Significant (P < 0.05) increases in absolute and relative pituitary weights occurred in ghrelin-treated rats versus controls (58% and 41%, respectively). Morphometric parameters (i.e., the volume of GH cells, volume of their nuclei, and volume density) all significantly (P < 0.05) increased by 17%, 18%, and 19%, respectively, in the ghrelin-treated group versus controls. Terminal serum concentration of GH was significantly (P < 0.05) increased by 15% with ghrelin treatment. The results clearly document that daily nanomolar doses of ghrelin into the lateral cerebral ventricle stimulate GH cell proliferation and promote GH release. Thus, achieving pharmacologic control of central ghrelin receptors is a promising modality to modulate the actions of GH.
T2  - Experimental Biology and Medicine
T1  - Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats
IS  - 10
VL  - 231
EP  - 1615
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1629
ER  - 
@article{
author = "Stevanović, Darko M and Milošević, Verica and Nesić, Dejan M and Ajdžanović, Vladimir and Starčević, Vesna P. and Severs, Walter B",
year = "2006",
abstract = "Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally purified from rat stomach; subsequently, ghrelin neurons were found in the arcuate nuclei of rats. Central effects of the peptide on GH release, however, remain to be clarified. The aim of the present study was to determine the morphologic features of GH-producing pituicytes and serum GH concentration after central administration of ghrelin. Five injections of rat ghrelin or phosphate-buffered saline (PBS; n = 10 rats/group) were given every 24 hrs (1 mu g of ghrelin in 5 mu l of PBS) into the lateral cerebral ventricle of male rats. Significant (P < 0.05) increases in absolute and relative pituitary weights occurred in ghrelin-treated rats versus controls (58% and 41%, respectively). Morphometric parameters (i.e., the volume of GH cells, volume of their nuclei, and volume density) all significantly (P < 0.05) increased by 17%, 18%, and 19%, respectively, in the ghrelin-treated group versus controls. Terminal serum concentration of GH was significantly (P < 0.05) increased by 15% with ghrelin treatment. The results clearly document that daily nanomolar doses of ghrelin into the lateral cerebral ventricle stimulate GH cell proliferation and promote GH release. Thus, achieving pharmacologic control of central ghrelin receptors is a promising modality to modulate the actions of GH.",
journal = "Experimental Biology and Medicine",
title = "Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats",
number = "10",
volume = "231",
pages = "1615",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1629"
}
Stevanović, D. M., Milošević, V., Nesić, D. M., Ajdžanović, V., Starčević, V. P.,& Severs, W. B.. (2006). Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats. in Experimental Biology and Medicine, 231(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1629
Stevanović DM, Milošević V, Nesić DM, Ajdžanović V, Starčević VP, Severs WB. Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats. in Experimental Biology and Medicine. 2006;231(10):null-1615.
https://hdl.handle.net/21.15107/rcub_ibiss_1629 .
Stevanović, Darko M, Milošević, Verica, Nesić, Dejan M, Ajdžanović, Vladimir, Starčević, Vesna P., Severs, Walter B, "Central effects of ghrelin on serum growth hormone and morphology of pituitary somatotropes in rats" in Experimental Biology and Medicine, 231, no. 10 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1629 .