TY  - JOUR
AU  - Babotă, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Rocchetti, Gabriele
AU  - Zhang, Leilei
AU  - Lucini, Luigi
AU  - Crișan, Gianina
AU  - Mocan, Andrei
AU  - Vostinaru, Oliviu
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5502
AB  - Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.
PB  - Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
VL  - 14
DO  - 10.3389/fphar.2023.1115117
SP  - 1115117
ER  - 

@article{
author = "Babotă, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Stojković, Dejan and Soković, Marina and Rocchetti, Gabriele and Zhang, Leilei and Lucini, Luigi and Crișan, Gianina and Mocan, Andrei and Vostinaru, Oliviu",
year = "2023",
abstract = "Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania",
volume = "14",
doi = "10.3389/fphar.2023.1115117",
pages = "1115117"
}

Babotă, M., Frumuzachi, O., Nicolescu, A., Stojković, D., Soković, M., Rocchetti, G., Zhang, L., Lucini, L., Crișan, G., Mocan, A.,& Vostinaru, O.. (2023). Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology
Frontiers Media S.A.., 14, 1115117.
https://doi.org/10.3389/fphar.2023.1115117

Babotă M, Frumuzachi O, Nicolescu A, Stojković D, Soković M, Rocchetti G, Zhang L, Lucini L, Crișan G, Mocan A, Vostinaru O. Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology. 2023;14:1115117.
doi:10.3389/fphar.2023.1115117 .

Babotă, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Stojković, Dejan, Soković, Marina, Rocchetti, Gabriele, Zhang, Leilei, Lucini, Luigi, Crișan, Gianina, Mocan, Andrei, Vostinaru, Oliviu, "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania" in Frontiers in Pharmacology, 14 (2023):1115117,
https://doi.org/10.3389/fphar.2023.1115117 . .

TY  - JOUR
AU  - Babota, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Barros, Lillian
AU  - Añibarro-Ortega, Mikel
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Mocan, Andrei
AU  - López, Víctor
AU  - Crișan, Gianina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5475
AB  - Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020390
SP  - 390
ER  - 

@article{
author = "Babota, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Dias, Maria Inês and Pinela, José and Barros, Lillian and Añibarro-Ortega, Mikel and Stojković, Dejan and Carević, Tamara and Mocan, Andrei and López, Víctor and Crișan, Gianina",
year = "2023",
abstract = "Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits",
number = "2",
volume = "12",
doi = "10.3390/antiox12020390",
pages = "390"
}

Babota, M., Frumuzachi, O., Nicolescu, A., Dias, M. I., Pinela, J., Barros, L., Añibarro-Ortega, M., Stojković, D., Carević, T., Mocan, A., López, V.,& Crișan, G.. (2023). Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants
Basel: MDPI., 12(2), 390.
https://doi.org/10.3390/antiox12020390

Babota M, Frumuzachi O, Nicolescu A, Dias MI, Pinela J, Barros L, Añibarro-Ortega M, Stojković D, Carević T, Mocan A, López V, Crișan G. Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants. 2023;12(2):390.
doi:10.3390/antiox12020390 .

Babota, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Dias, Maria Inês, Pinela, José, Barros, Lillian, Añibarro-Ortega, Mikel, Stojković, Dejan, Carević, Tamara, Mocan, Andrei, López, Víctor, Crișan, Gianina, "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits" in Antioxidants, 12, no. 2 (2023):390,
https://doi.org/10.3390/antiox12020390 . .

TY  - JOUR
AU  - Babotă, Mihai
AU  - Frumuzachi, Oleg
AU  - Mocan, Andrei
AU  - Tămaș, Mircea
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Bădărău, Alexandru Sabin
AU  - Crișan, Gianina
AU  - Barros, Lillian
AU  - Păltinean, Ramona
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/20/2773
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5180
AB  - Hypericum perforatum L., also known as St. John’s Wort, is recognized worldwide as a valuable medicinal herb; however, other Hypericum species were intensively studied for their bioactive potential. To fill the research gap that exists in the scientific literature, a comparative evaluation between H. alpigenum Kit., H. perforatum L. and H. rochelii Griseb. & Schenk was conducted in the present study. Two types of herbal preparations obtained from the aerial parts of these species were analyzed: extracts obtained through maceration and extracts obtained through magnetic-stirring-assisted extraction. LC-DAD-ESI-MSn analysis revealed the presence of phenolic acids, flavan-3-ols and flavonoid derivatives as the main constituents of the above-mentioned species. Moreover, all extracts were tested for their antioxidant, enzyme-inhibitory and antimicrobial potential. Our work emphasizes for the first time a detailed description of H. rochelii phenolic fractions, including their phytochemical and bioactive characterization. In comparison with the other two studied species, H. rochelii was found as a rich source of phenolic acids and myricetin derivatives, showing important antioxidant, anticholinesterase and antibacterial activity. The study offers new perspectives regarding the chemical and bioactive profile of the less-studied species H. alpigenum and H. rochelii.
PB  - Basel: MDPI
T2  - Plants
T1  - Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii
IS  - 20
VL  - 11
DO  - 10.3390/plants11202773
SP  - 2773
ER  - 

@article{
author = "Babotă, Mihai and Frumuzachi, Oleg and Mocan, Andrei and Tămaș, Mircea and Dias, Maria Inês and Pinela, José and Stojković, Dejan and Soković, Marina and Bădărău, Alexandru Sabin and Crișan, Gianina and Barros, Lillian and Păltinean, Ramona",
year = "2022",
abstract = "Hypericum perforatum L., also known as St. John’s Wort, is recognized worldwide as a valuable medicinal herb; however, other Hypericum species were intensively studied for their bioactive potential. To fill the research gap that exists in the scientific literature, a comparative evaluation between H. alpigenum Kit., H. perforatum L. and H. rochelii Griseb. & Schenk was conducted in the present study. Two types of herbal preparations obtained from the aerial parts of these species were analyzed: extracts obtained through maceration and extracts obtained through magnetic-stirring-assisted extraction. LC-DAD-ESI-MSn analysis revealed the presence of phenolic acids, flavan-3-ols and flavonoid derivatives as the main constituents of the above-mentioned species. Moreover, all extracts were tested for their antioxidant, enzyme-inhibitory and antimicrobial potential. Our work emphasizes for the first time a detailed description of H. rochelii phenolic fractions, including their phytochemical and bioactive characterization. In comparison with the other two studied species, H. rochelii was found as a rich source of phenolic acids and myricetin derivatives, showing important antioxidant, anticholinesterase and antibacterial activity. The study offers new perspectives regarding the chemical and bioactive profile of the less-studied species H. alpigenum and H. rochelii.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii",
number = "20",
volume = "11",
doi = "10.3390/plants11202773",
pages = "2773"
}

Babotă, M., Frumuzachi, O., Mocan, A., Tămaș, M., Dias, M. I., Pinela, J., Stojković, D., Soković, M., Bădărău, A. S., Crișan, G., Barros, L.,& Păltinean, R.. (2022). Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii. in Plants
Basel: MDPI., 11(20), 2773.
https://doi.org/10.3390/plants11202773

Babotă M, Frumuzachi O, Mocan A, Tămaș M, Dias MI, Pinela J, Stojković D, Soković M, Bădărău AS, Crișan G, Barros L, Păltinean R. Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii. in Plants. 2022;11(20):2773.
doi:10.3390/plants11202773 .

Babotă, Mihai, Frumuzachi, Oleg, Mocan, Andrei, Tămaș, Mircea, Dias, Maria Inês, Pinela, José, Stojković, Dejan, Soković, Marina, Bădărău, Alexandru Sabin, Crișan, Gianina, Barros, Lillian, Păltinean, Ramona, "Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H. perforatum and H. rochelii" in Plants, 11, no. 20 (2022):2773,
https://doi.org/10.3390/plants11202773 . .

TY  - JOUR
AU  - Mocan, Andrei
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Gavrilaş, Laura
AU  - Ferreira, Isabel C. F. R.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Babotă, Mihai
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.881210/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9168797
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5006
AB  - The members of Cystus genus are perenial shrubs with a well-established use in traditional medicine. Among these, C. creticus is the most popular, herbal preparations obtained from its aerial parts being recognized as antimicrobial, antitumor and anti-inflammatory agents. The present study aimed to evaluate phytochemical profile and bioactive potential of aqueous and hydroethanolic extracts of C. creticus aerial parts harvested from two different areas of Rhodes island. LC-DAD-ESI/MSn analysis revealed the presence of myricetin and quercetin glycosides as main compounds, especially in aqueous extracts, being probably responsible for their enhanced antioxidant and antimicrobial potential. On the other side, hydroethanolic preparations exerted a strong anti-inflammatory and anti-biofilm activity. Our findings suggest that the use of solvents with intermediate polarity can assure the best recovery of bioactive compounds from C. creticus, increasing the extraction yield for other non-phenolic compounds which can enhance therapeutic potential of the extract through a synergistic action.
T2  - Frontiers in Nutrition
T1  - Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.
VL  - 9
DO  - 10.3389/fnut.2022.881210
SP  - 881210
ER  - 

@article{
author = "Mocan, Andrei and Fernandes, Ângela and Calhelha, Ricardo C. and Gavrilaş, Laura and Ferreira, Isabel C. F. R. and Ivanov, Marija and Soković, Marina and Barros, Lillian and Babotă, Mihai",
year = "2022",
abstract = "The members of Cystus genus are perenial shrubs with a well-established use in traditional medicine. Among these, C. creticus is the most popular, herbal preparations obtained from its aerial parts being recognized as antimicrobial, antitumor and anti-inflammatory agents. The present study aimed to evaluate phytochemical profile and bioactive potential of aqueous and hydroethanolic extracts of C. creticus aerial parts harvested from two different areas of Rhodes island. LC-DAD-ESI/MSn analysis revealed the presence of myricetin and quercetin glycosides as main compounds, especially in aqueous extracts, being probably responsible for their enhanced antioxidant and antimicrobial potential. On the other side, hydroethanolic preparations exerted a strong anti-inflammatory and anti-biofilm activity. Our findings suggest that the use of solvents with intermediate polarity can assure the best recovery of bioactive compounds from C. creticus, increasing the extraction yield for other non-phenolic compounds which can enhance therapeutic potential of the extract through a synergistic action.",
journal = "Frontiers in Nutrition",
title = "Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.",
volume = "9",
doi = "10.3389/fnut.2022.881210",
pages = "881210"
}

Mocan, A., Fernandes, Â., Calhelha, R. C., Gavrilaş, L., Ferreira, I. C. F. R., Ivanov, M., Soković, M., Barros, L.,& Babotă, M.. (2022). Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.. in Frontiers in Nutrition, 9, 881210.
https://doi.org/10.3389/fnut.2022.881210

Mocan A, Fernandes Â, Calhelha RC, Gavrilaş L, Ferreira ICFR, Ivanov M, Soković M, Barros L, Babotă M. Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.. in Frontiers in Nutrition. 2022;9:881210.
doi:10.3389/fnut.2022.881210 .

Mocan, Andrei, Fernandes, Ângela, Calhelha, Ricardo C., Gavrilaş, Laura, Ferreira, Isabel C. F. R., Ivanov, Marija, Soković, Marina, Barros, Lillian, Babotă, Mihai, "Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island." in Frontiers in Nutrition, 9 (2022):881210,
https://doi.org/10.3389/fnut.2022.881210 . .

TY  - JOUR
AU  - Mocan, Andrei
AU  - Babota, Mihai
AU  - Pop, Anca
AU  - Fizeșan, Ionel
AU  - Diuzheva, Alina
AU  - Locatelli, Marcello
AU  - Carradori, Simone
AU  - Campestre, Cristina
AU  - Menghini, Luigi
AU  - Sisea, Cristian R.
AU  - Soković, Marina
AU  - Zengin, Gokhan
AU  - Păltinean, Ramona
AU  - Badarau, Sabin
AU  - C Vodnar, Dan C
AU  - Crișan, Gianina
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/6/480
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32498441
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3703
AB  - Even though Salvia genus is one of the most known and studied taxa of Lamiaceae family, the knowledge regarding the chemical composition and health-related benefits of some locally used Salvia species (mostly endemic) is still scarce. In this regard, the present work aims to evaluate the chemical profile and potential bioactivities of 70% (v/v) ethanolic extracts obtained from the less-studied S. transsylvanica and S. glutinosa in comparison with S. officinalis. HPLC-PDA analysis revealed the presence of rutin and catechin as the main compounds in the extracts of the three studied species (using the employed HPLC method), whereas the presence of naringenin was highlighted only in S. glutinosa extract. Chlorogenic acid, rutin and quercetin were identified and quantified for the first time in S. transsylvanica extracts. The in vitro antioxidant capacity of each extract was tested through complementary methods (phosphomolybdenum assay, DPPH, ABTS, CUPRAC and FRAP assays), and correlated with the presence of phenolics (especially flavonoids) in high amounts. The neuroprotective and antidiabetic abilities of S. officinalis (the most active as AChE, BChE and α-glucosidase inhibitor), S. glutinosa (the most active as α-amylase inhibitor) and S. transsylvanica were also studied. For each extract it was determined the antimicrobial, antifungal and cytotoxic effects using in vitro assays. The obtained results confirm the potential of S. transsylvanica and S. glutinosa as promising sources of bioactive compounds and as a starting point for further analyses.
PB  - MDPI AG
T2  - Antioxidants (Basel, Switzerland)
T1  - Chemical Constituents and Biologic Activities of Sage Species: A Comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur.
IS  - 6
VL  - 9
DO  - 10.3390/antiox9060480
SP  - 480
ER  - 

@article{
author = "Mocan, Andrei and Babota, Mihai and Pop, Anca and Fizeșan, Ionel and Diuzheva, Alina and Locatelli, Marcello and Carradori, Simone and Campestre, Cristina and Menghini, Luigi and Sisea, Cristian R. and Soković, Marina and Zengin, Gokhan and Păltinean, Ramona and Badarau, Sabin and C Vodnar, Dan C and Crișan, Gianina",
year = "2020",
abstract = "Even though Salvia genus is one of the most known and studied taxa of Lamiaceae family, the knowledge regarding the chemical composition and health-related benefits of some locally used Salvia species (mostly endemic) is still scarce. In this regard, the present work aims to evaluate the chemical profile and potential bioactivities of 70% (v/v) ethanolic extracts obtained from the less-studied S. transsylvanica and S. glutinosa in comparison with S. officinalis. HPLC-PDA analysis revealed the presence of rutin and catechin as the main compounds in the extracts of the three studied species (using the employed HPLC method), whereas the presence of naringenin was highlighted only in S. glutinosa extract. Chlorogenic acid, rutin and quercetin were identified and quantified for the first time in S. transsylvanica extracts. The in vitro antioxidant capacity of each extract was tested through complementary methods (phosphomolybdenum assay, DPPH, ABTS, CUPRAC and FRAP assays), and correlated with the presence of phenolics (especially flavonoids) in high amounts. The neuroprotective and antidiabetic abilities of S. officinalis (the most active as AChE, BChE and α-glucosidase inhibitor), S. glutinosa (the most active as α-amylase inhibitor) and S. transsylvanica were also studied. For each extract it was determined the antimicrobial, antifungal and cytotoxic effects using in vitro assays. The obtained results confirm the potential of S. transsylvanica and S. glutinosa as promising sources of bioactive compounds and as a starting point for further analyses.",
publisher = "MDPI AG",
journal = "Antioxidants (Basel, Switzerland)",
title = "Chemical Constituents and Biologic Activities of Sage Species: A Comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur.",
number = "6",
volume = "9",
doi = "10.3390/antiox9060480",
pages = "480"
}

Mocan, A., Babota, M., Pop, A., Fizeșan, I., Diuzheva, A., Locatelli, M., Carradori, S., Campestre, C., Menghini, L., Sisea, C. R., Soković, M., Zengin, G., Păltinean, R., Badarau, S., C Vodnar, D. C.,& Crișan, G.. (2020). Chemical Constituents and Biologic Activities of Sage Species: A Comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur.. in Antioxidants (Basel, Switzerland)
MDPI AG., 9(6), 480.
https://doi.org/10.3390/antiox9060480

Mocan A, Babota M, Pop A, Fizeșan I, Diuzheva A, Locatelli M, Carradori S, Campestre C, Menghini L, Sisea CR, Soković M, Zengin G, Păltinean R, Badarau S, C Vodnar DC, Crișan G. Chemical Constituents and Biologic Activities of Sage Species: A Comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur.. in Antioxidants (Basel, Switzerland). 2020;9(6):480.
doi:10.3390/antiox9060480 .

Mocan, Andrei, Babota, Mihai, Pop, Anca, Fizeșan, Ionel, Diuzheva, Alina, Locatelli, Marcello, Carradori, Simone, Campestre, Cristina, Menghini, Luigi, Sisea, Cristian R., Soković, Marina, Zengin, Gokhan, Păltinean, Ramona, Badarau, Sabin, C Vodnar, Dan C, Crișan, Gianina, "Chemical Constituents and Biologic Activities of Sage Species: A Comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur." in Antioxidants (Basel, Switzerland), 9, no. 6 (2020):480,
https://doi.org/10.3390/antiox9060480 . .

TY  - JOUR
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Barros, Lillian
AU  - Ćirić, Ana
AU  - Silva, Soraia P.
AU  - Coelho, Elisabete
AU  - Mocan, Andrei
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Coimbra, Manuel A.
AU  - Ferreira, Isabel C. F. R.
PY  - 2019
UR  - https://www.mdpi.com/2076-3921/8/10/444
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3491
AB  - This work aimed to characterize compositional and bioactive features of Aloe vera leaf (fillet, mucilage, and rind) and flower. The edible fillet was analysed for its nutritional value, and all samples were studied for phenolic composition and antioxidant, anti-inflammatory, antimicrobial, tyrosinase inhibition, and cytotoxic activities. Dietary fibre (mainly mannan) and available carbohydrates (mainly free glucose and fructose) were abundant macronutrients in fillet, which also contained high amounts of malic acid (5.75 g/100 g dw) and α-tocopherol (4.8 mg/100 g dw). The leaf samples presented similar phenolic profiles, with predominance of chromones and anthrones, and the highest contents were found in mucilage (131 mg/g) and rind (105 mg/g) extracts, which also revealed interesting antioxidant properties. On the other hand, the flower extract was rich in apigenin glycoside derivatives (4.48 mg/g), effective against Pseudomonas aeruginosa (MIC = 0.025 mg/mL and MBC = 0.05 mg/mL) and capable of inhibiting the tyrosinase activity (IC50 = 4.85 mg/mL). The fillet, rind, and flower extracts also showed a powerful antifungal activity against Aspergillus flavus, A. niger, Penicillium funiculosum, and Candida albicans, higher than that of ketoconazole. Thus, the studied Aloe vera samples displayed high potential to be exploited by the food or cosmetic industries, among others.
T2  - Antioxidants (Basel, Switzerland)
T1  - Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower
IS  - 10
VL  - 8
DO  - 10.3390/antiox8100444
SP  - 444
ER  - 

@article{
author = "Añibarro-Ortega, Mikel and Pinela, José and Barros, Lillian and Ćirić, Ana and Silva, Soraia P. and Coelho, Elisabete and Mocan, Andrei and Calhelha, Ricardo C. and Soković, Marina and Coimbra, Manuel A. and Ferreira, Isabel C. F. R.",
year = "2019",
abstract = "This work aimed to characterize compositional and bioactive features of Aloe vera leaf (fillet, mucilage, and rind) and flower. The edible fillet was analysed for its nutritional value, and all samples were studied for phenolic composition and antioxidant, anti-inflammatory, antimicrobial, tyrosinase inhibition, and cytotoxic activities. Dietary fibre (mainly mannan) and available carbohydrates (mainly free glucose and fructose) were abundant macronutrients in fillet, which also contained high amounts of malic acid (5.75 g/100 g dw) and α-tocopherol (4.8 mg/100 g dw). The leaf samples presented similar phenolic profiles, with predominance of chromones and anthrones, and the highest contents were found in mucilage (131 mg/g) and rind (105 mg/g) extracts, which also revealed interesting antioxidant properties. On the other hand, the flower extract was rich in apigenin glycoside derivatives (4.48 mg/g), effective against Pseudomonas aeruginosa (MIC = 0.025 mg/mL and MBC = 0.05 mg/mL) and capable of inhibiting the tyrosinase activity (IC50 = 4.85 mg/mL). The fillet, rind, and flower extracts also showed a powerful antifungal activity against Aspergillus flavus, A. niger, Penicillium funiculosum, and Candida albicans, higher than that of ketoconazole. Thus, the studied Aloe vera samples displayed high potential to be exploited by the food or cosmetic industries, among others.",
journal = "Antioxidants (Basel, Switzerland)",
title = "Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower",
number = "10",
volume = "8",
doi = "10.3390/antiox8100444",
pages = "444"
}

Añibarro-Ortega, M., Pinela, J., Barros, L., Ćirić, A., Silva, S. P., Coelho, E., Mocan, A., Calhelha, R. C., Soković, M., Coimbra, M. A.,& Ferreira, I. C. F. R.. (2019). Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower. in Antioxidants (Basel, Switzerland), 8(10), 444.
https://doi.org/10.3390/antiox8100444

Añibarro-Ortega M, Pinela J, Barros L, Ćirić A, Silva SP, Coelho E, Mocan A, Calhelha RC, Soković M, Coimbra MA, Ferreira ICFR. Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower. in Antioxidants (Basel, Switzerland). 2019;8(10):444.
doi:10.3390/antiox8100444 .

Añibarro-Ortega, Mikel, Pinela, José, Barros, Lillian, Ćirić, Ana, Silva, Soraia P., Coelho, Elisabete, Mocan, Andrei, Calhelha, Ricardo C., Soković, Marina, Coimbra, Manuel A., Ferreira, Isabel C. F. R., "Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower" in Antioxidants (Basel, Switzerland), 8, no. 10 (2019):444,
https://doi.org/10.3390/antiox8100444 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Zheleva-Dimitrova, Dimitrina
AU  - Gevrenova, Reneta
AU  - Nedialkov, Paraskev
AU  - Mocan, Andrei
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, M.Fawzi
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0731708517325074
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2931
AB  - The Centaurea genus has great potential in traditional systems and has attracted much interest in the design of novel drug formulations. The present study was focused on the chemical fingerprints and biological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spectrophotometric and LC-MS techniques were used to establish the chemical profiles of the studied extracts. Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namely neurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes (α-amylase and α-glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterial and fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPH and ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metal chelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid content of the extracts were in the range of 2.88-22.39 mgRE/g extract. Methanol and water extracts showed stronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts were most efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted considerable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifolia subsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open new avenues for research and contribute towards establishing primary data on these species for designing novel phytopharmaceuticals.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia
VL  - 149
DO  - 10.1016/j.jpba.2017.11.045
SP  - 436
EP  - 441
ER  - 

@article{
author = "Zengin, Gokhan and Zheleva-Dimitrova, Dimitrina and Gevrenova, Reneta and Nedialkov, Paraskev and Mocan, Andrei and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Aktumsek, Abdurrahman and Mahomoodally, M.Fawzi",
year = "2018",
abstract = "The Centaurea genus has great potential in traditional systems and has attracted much interest in the design of novel drug formulations. The present study was focused on the chemical fingerprints and biological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spectrophotometric and LC-MS techniques were used to establish the chemical profiles of the studied extracts. Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namely neurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes (α-amylase and α-glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterial and fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPH and ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metal chelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid content of the extracts were in the range of 2.88-22.39 mgRE/g extract. Methanol and water extracts showed stronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts were most efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted considerable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifolia subsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open new avenues for research and contribute towards establishing primary data on these species for designing novel phytopharmaceuticals.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia",
volume = "149",
doi = "10.1016/j.jpba.2017.11.045",
pages = "436-441"
}

Zengin, G., Zheleva-Dimitrova, D., Gevrenova, R., Nedialkov, P., Mocan, A., Ćirić, A., Glamočlija, J., Soković, M., Aktumsek, A.,& Mahomoodally, M.Fawzi. (2018). Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis, 149, 436-441.
https://doi.org/10.1016/j.jpba.2017.11.045

Zengin G, Zheleva-Dimitrova D, Gevrenova R, Nedialkov P, Mocan A, Ćirić A, Glamočlija J, Soković M, Aktumsek A, Mahomoodally M. Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis. 2018;149:436-441.
doi:10.1016/j.jpba.2017.11.045 .

Zengin, Gokhan, Zheleva-Dimitrova, Dimitrina, Gevrenova, Reneta, Nedialkov, Paraskev, Mocan, Andrei, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Aktumsek, Abdurrahman, Mahomoodally, M.Fawzi, "Identification of phenolic components via LC-MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia" in Journal of Pharmaceutical and Biomedical Analysis, 149 (2018):436-441,
https://doi.org/10.1016/j.jpba.2017.11.045 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Mahomoodally, M. Fawzi
AU  - Aktumsek, Abdurrahman
AU  - Ceylan, Ramazan
AU  - Uysal, Sengul
AU  - Mocan, Andrei
AU  - Yilmaz, Mustafa Abdullah
AU  - Picot-Allain, Carene Marie Nancy
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S2212429217305473?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3090
AB  - Six wild species (S. alba, S. conoidea, S. dichotoma, S. italica, S. supina, and S. vulgaris) from the Silene genera were tested for potential anti-enzymatic (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase), antimicrobial (16 microbial strains), and antioxidant activity. An ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method was used for phytochemical determination. Quinic acid, malic acid, protocatechuic acid, p-coumaric acid, and hesperidin were common in the six Silene species. All extracts showed higher antibacterial effects compared to streptomycin and ampicillin (except S. dichotoma). Antifungal agents, bifonazole (MIC 0.10–0.20 mg/mL and MFC 0.20–0.30 mg/mL) and ketoconazole (MIC 0.15–2.30 mg/mL and MFC 0.20–3.50 mg/mL) showed lower activity than the investigated Silene species extracts. S. alba inhibited AChE (2.00 mg GALAE/g extract) and BChE (1.0 mg GALAE/g extract). The results showed metal chelating potential ranging from 12 to 19 mg EDTAE/g extract, with S. conoidea being the most active, and S. supina the least. S. dichotoma showed the highest reducing potential against both cupric (154 mg TE/g extract for CUPRAC) and ferric (102 mg TE/g extract for FRAP) ions. Overall, Silene species could be considered as emerging interesting functional foods and sources of nutraceuticals with applications in the management of different diseases.
T2  - Food Bioscience
T1  - Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties
VL  - 23
DO  - 10.1016/J.FBIO.2018.03.010
SP  - 75
EP  - 82
ER  - 

@article{
author = "Zengin, Gokhan and Mahomoodally, M. Fawzi and Aktumsek, Abdurrahman and Ceylan, Ramazan and Uysal, Sengul and Mocan, Andrei and Yilmaz, Mustafa Abdullah and Picot-Allain, Carene Marie Nancy and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2018",
abstract = "Six wild species (S. alba, S. conoidea, S. dichotoma, S. italica, S. supina, and S. vulgaris) from the Silene genera were tested for potential anti-enzymatic (acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase), antimicrobial (16 microbial strains), and antioxidant activity. An ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method was used for phytochemical determination. Quinic acid, malic acid, protocatechuic acid, p-coumaric acid, and hesperidin were common in the six Silene species. All extracts showed higher antibacterial effects compared to streptomycin and ampicillin (except S. dichotoma). Antifungal agents, bifonazole (MIC 0.10–0.20 mg/mL and MFC 0.20–0.30 mg/mL) and ketoconazole (MIC 0.15–2.30 mg/mL and MFC 0.20–3.50 mg/mL) showed lower activity than the investigated Silene species extracts. S. alba inhibited AChE (2.00 mg GALAE/g extract) and BChE (1.0 mg GALAE/g extract). The results showed metal chelating potential ranging from 12 to 19 mg EDTAE/g extract, with S. conoidea being the most active, and S. supina the least. S. dichotoma showed the highest reducing potential against both cupric (154 mg TE/g extract for CUPRAC) and ferric (102 mg TE/g extract for FRAP) ions. Overall, Silene species could be considered as emerging interesting functional foods and sources of nutraceuticals with applications in the management of different diseases.",
journal = "Food Bioscience",
title = "Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties",
volume = "23",
doi = "10.1016/J.FBIO.2018.03.010",
pages = "75-82"
}

Zengin, G., Mahomoodally, M. F., Aktumsek, A., Ceylan, R., Uysal, S., Mocan, A., Yilmaz, M. A., Picot-Allain, C. M. N., Ćirić, A., Glamočlija, J.,& Soković, M.. (2018). Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties. in Food Bioscience, 23, 75-82.
https://doi.org/10.1016/J.FBIO.2018.03.010

Zengin G, Mahomoodally MF, Aktumsek A, Ceylan R, Uysal S, Mocan A, Yilmaz MA, Picot-Allain CMN, Ćirić A, Glamočlija J, Soković M. Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties. in Food Bioscience. 2018;23:75-82.
doi:10.1016/J.FBIO.2018.03.010 .

Zengin, Gokhan, Mahomoodally, M. Fawzi, Aktumsek, Abdurrahman, Ceylan, Ramazan, Uysal, Sengul, Mocan, Andrei, Yilmaz, Mustafa Abdullah, Picot-Allain, Carene Marie Nancy, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Functional constituents of six wild edible Silene species: A focus on their phytochemical profiles and bioactive properties" in Food Bioscience, 23 (2018):75-82,
https://doi.org/10.1016/J.FBIO.2018.03.010 . .

TY  - JOUR
AU  - Mocan, Andrei
AU  - Fernandes, Ângela
AU  - Barros, Lillian
AU  - Crişan, Gianina
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C7FO01514C
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29168866
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2996
AB  - In Eastern Europe, wild mushrooms are widely collected in mountain areas and used for their medicinal properties or as healthy foods. This study aimed at determining the chemical composition (nutritional value, free sugars, organic acids, phenolic compounds, fatty acids and tocopherols) and bioactive properties (antioxidant, antimicrobial and antiquorum sensing) of wild Polyporus squamosus (Huds.) Fr from Romania. The results indicate that the fruiting bodies of P. squamosus are rich in carbohydrates (74.22 g per 100 g dw) and proteins (18.7 g per 100 g dw). Trehalose was the main free sugar, while malic acid was the organic acid detected in the highest amount (2.21 g per 100 g dw), and p-hydroxybenzoic acid was the main phenolic compound. Among tocopherols, β-tocopherol was the most abundant form (114.7 μg per 100 g dw). Additionally, regarding the fatty acids' pattern, polyunsaturated acids represent more than 57% of all fatty acids, followed by monounsaturated fatty acids (24.96%). The highest measured antioxidant effect of P. squamosus extract was found using the TBARS inhibition assay (EC50= 0.22 mg mL-1), followed by the β-carotene/linoleate assay (EC50= 1.41 mg mL-1). A minimal inhibitory concentration of the tested extracts was obtained between 0.61-20.4 mg mL-1, while the bactericidal effect was achieved between 1.2-40.8 mg mL-1. Antibiofilm potential was obtained at all tested concentrations, and subinhibitory concentrations of the extract exhibited an antiquorum effect and reduced the formation of P. aeruginosa pili, which all together influenced the virulence of this bacterium. Due to the investigated bioactivities and compounds of P. squamosus and its well-balanced nutritional profile, this mushroom can be further used as a medicinal ingredient based on its antioxidative and antimicrobial potential.
T2  - Food & Function
T1  - Chemical composition and bioactive properties of the wild mushroom Polyporus squamosus (Huds.) Fr: a study with samples from Romania.
IS  - 1
VL  - 9
DO  - 10.1039/c7fo01514c
SP  - 160
EP  - 170
ER  - 

@article{
author = "Mocan, Andrei and Fernandes, Ângela and Barros, Lillian and Crişan, Gianina and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R.",
year = "2018",
abstract = "In Eastern Europe, wild mushrooms are widely collected in mountain areas and used for their medicinal properties or as healthy foods. This study aimed at determining the chemical composition (nutritional value, free sugars, organic acids, phenolic compounds, fatty acids and tocopherols) and bioactive properties (antioxidant, antimicrobial and antiquorum sensing) of wild Polyporus squamosus (Huds.) Fr from Romania. The results indicate that the fruiting bodies of P. squamosus are rich in carbohydrates (74.22 g per 100 g dw) and proteins (18.7 g per 100 g dw). Trehalose was the main free sugar, while malic acid was the organic acid detected in the highest amount (2.21 g per 100 g dw), and p-hydroxybenzoic acid was the main phenolic compound. Among tocopherols, β-tocopherol was the most abundant form (114.7 μg per 100 g dw). Additionally, regarding the fatty acids' pattern, polyunsaturated acids represent more than 57% of all fatty acids, followed by monounsaturated fatty acids (24.96%). The highest measured antioxidant effect of P. squamosus extract was found using the TBARS inhibition assay (EC50= 0.22 mg mL-1), followed by the β-carotene/linoleate assay (EC50= 1.41 mg mL-1). A minimal inhibitory concentration of the tested extracts was obtained between 0.61-20.4 mg mL-1, while the bactericidal effect was achieved between 1.2-40.8 mg mL-1. Antibiofilm potential was obtained at all tested concentrations, and subinhibitory concentrations of the extract exhibited an antiquorum effect and reduced the formation of P. aeruginosa pili, which all together influenced the virulence of this bacterium. Due to the investigated bioactivities and compounds of P. squamosus and its well-balanced nutritional profile, this mushroom can be further used as a medicinal ingredient based on its antioxidative and antimicrobial potential.",
journal = "Food & Function",
title = "Chemical composition and bioactive properties of the wild mushroom Polyporus squamosus (Huds.) Fr: a study with samples from Romania.",
number = "1",
volume = "9",
doi = "10.1039/c7fo01514c",
pages = "160-170"
}

Mocan, A., Fernandes, Â., Barros, L., Crişan, G., Ivanov, M., Soković, M.,& Ferreira, I. C. F. R.. (2018). Chemical composition and bioactive properties of the wild mushroom Polyporus squamosus (Huds.) Fr: a study with samples from Romania.. in Food & Function, 9(1), 160-170.
https://doi.org/10.1039/c7fo01514c

Mocan A, Fernandes Â, Barros L, Crişan G, Ivanov M, Soković M, Ferreira ICFR. Chemical composition and bioactive properties of the wild mushroom Polyporus squamosus (Huds.) Fr: a study with samples from Romania.. in Food & Function. 2018;9(1):160-170.
doi:10.1039/c7fo01514c .

Mocan, Andrei, Fernandes, Ângela, Barros, Lillian, Crişan, Gianina, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., "Chemical composition and bioactive properties of the wild mushroom Polyporus squamosus (Huds.) Fr: a study with samples from Romania." in Food & Function, 9, no. 1 (2018):160-170,
https://doi.org/10.1039/c7fo01514c . .

TY  - JOUR
AU  - Uysal, Sengul
AU  - Aumeeruddy-Elalfi, Zaahira
AU  - Zengin, Gokhan
AU  - Aktumsek, Abdurrahman
AU  - Mocan, Andrei
AU  - Custodio, Luisa
AU  - Neng, Nuno R.
AU  - Nogueira, José M.F.
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Mahomoodally, M.Fawzi
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0926669018300013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2965
AB  - Ballota macrodonta Boiss. et Balansa., is a traditionally used endemic medicinal plant used to manage a plethora of diseases in Turkey. Nonetheless, few studies have endeavoured to highlight its therapeutic potential and its phytochemical profile. We hypothesized that B. macrodonta would possess multi-pharmacological propensities and bioactive compounds that would justify its use as a folk remedy. Aerial part of B. macrodonta was assessed for its antioxidant, antimicrobial, and enzymatic inhibitory potential using different extraction solvents. The total bioactive components determination and compound identification using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) method were used to correlate the biological properties. HPLC-DAD profile showed the presence of 19 components (e.g. hydroxybenzoic acid, catechin hydrate, vanillic acid, caffeic acid, syringic acid, epicatechin, coumaric acid, ferulic acid, luteolin-7-O-glucoside, rutin, and quercetin). The water and methanolic extracts showed high total phenolic and flavonoid contents, respectively. The overall antioxidant capacity of the water extract was higher than the one of the methanol extract. The extracts showed inhibitory activity against cholinesterases, tyrosinase, α-amylase and α-glucosidase. The extracts also presented antimicrobial activity against eight Gram positive/negative bacteria (MIC – 0.05–0.15 mg/mL) and eight fungi (MIC – 0.0125–0.20 mg/mL). The presence of active biomolecules with multi-pharmacological properties makes B. macrodonta a potential source of health-promoting compounds that can be exploited as a novel medicinal herbal product.
T2  - Industrial Crops and Products
T1  - Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey
VL  - 113
DO  - 10.1016/j.indcrop.2018.01.001
SP  - 422
EP  - 428
ER  - 

@article{
author = "Uysal, Sengul and Aumeeruddy-Elalfi, Zaahira and Zengin, Gokhan and Aktumsek, Abdurrahman and Mocan, Andrei and Custodio, Luisa and Neng, Nuno R. and Nogueira, José M.F. and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Mahomoodally, M.Fawzi",
year = "2018",
abstract = "Ballota macrodonta Boiss. et Balansa., is a traditionally used endemic medicinal plant used to manage a plethora of diseases in Turkey. Nonetheless, few studies have endeavoured to highlight its therapeutic potential and its phytochemical profile. We hypothesized that B. macrodonta would possess multi-pharmacological propensities and bioactive compounds that would justify its use as a folk remedy. Aerial part of B. macrodonta was assessed for its antioxidant, antimicrobial, and enzymatic inhibitory potential using different extraction solvents. The total bioactive components determination and compound identification using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) method were used to correlate the biological properties. HPLC-DAD profile showed the presence of 19 components (e.g. hydroxybenzoic acid, catechin hydrate, vanillic acid, caffeic acid, syringic acid, epicatechin, coumaric acid, ferulic acid, luteolin-7-O-glucoside, rutin, and quercetin). The water and methanolic extracts showed high total phenolic and flavonoid contents, respectively. The overall antioxidant capacity of the water extract was higher than the one of the methanol extract. The extracts showed inhibitory activity against cholinesterases, tyrosinase, α-amylase and α-glucosidase. The extracts also presented antimicrobial activity against eight Gram positive/negative bacteria (MIC – 0.05–0.15 mg/mL) and eight fungi (MIC – 0.0125–0.20 mg/mL). The presence of active biomolecules with multi-pharmacological properties makes B. macrodonta a potential source of health-promoting compounds that can be exploited as a novel medicinal herbal product.",
journal = "Industrial Crops and Products",
title = "Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey",
volume = "113",
doi = "10.1016/j.indcrop.2018.01.001",
pages = "422-428"
}

Uysal, S., Aumeeruddy-Elalfi, Z., Zengin, G., Aktumsek, A., Mocan, A., Custodio, L., Neng, N. R., Nogueira, J. M.F., Ćirić, A., Glamočlija, J., Soković, M.,& Mahomoodally, M.Fawzi. (2018). Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey. in Industrial Crops and Products, 113, 422-428.
https://doi.org/10.1016/j.indcrop.2018.01.001

Uysal S, Aumeeruddy-Elalfi Z, Zengin G, Aktumsek A, Mocan A, Custodio L, Neng NR, Nogueira JM, Ćirić A, Glamočlija J, Soković M, Mahomoodally M. Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey. in Industrial Crops and Products. 2018;113:422-428.
doi:10.1016/j.indcrop.2018.01.001 .

Uysal, Sengul, Aumeeruddy-Elalfi, Zaahira, Zengin, Gokhan, Aktumsek, Abdurrahman, Mocan, Andrei, Custodio, Luisa, Neng, Nuno R., Nogueira, José M.F., Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Mahomoodally, M.Fawzi, "Insight into the biological properties and phytochemical composition of Ballota macrodonta Boiss. et Balansa, — an endemic medicinal plant from Turkey" in Industrial Crops and Products, 113 (2018):422-428,
https://doi.org/10.1016/j.indcrop.2018.01.001 . .

TY  - JOUR
AU  - Zengin, Gökhan
AU  - Zheleva-Dimitrova, Dimitrina
AU  - Gevrenova, Reneta
AU  - Nedialkov, Paraskev
AU  - Mocan, Andrei
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, Muhammed Fettah
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4304
AB  - The Centaurea genus has great potential in traditional systems and has attracted much interest in thedesign of novel drug formulations. The present study was focused on the chemical fingerprints andbiological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spec-trophotometric and LC–MS techniques were used to establish the chemical profiles of the studied extracts.Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namelyneurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes ( -amylaseand  -glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterialand fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPHand ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metalchelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid con-tent of the extracts were in the range of 2.88–22.39 mgRE/g extract. Methanol and water extracts showedstronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts weremost efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted con-siderable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifoliasubsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open newavenues for research and contribute towards establishing primary data on these species for designingnovel phytopharmaceuticals.
PB  - Elsevier B.V.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia
VL  - 149
DO  - 10.1016/j.jpba.2017.11.045
SP  - 436
EP  - 441
ER  - 

@article{
author = "Zengin, Gökhan and Zheleva-Dimitrova, Dimitrina and Gevrenova, Reneta and Nedialkov, Paraskev and Mocan, Andrei and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Aktumsek, Abdurrahman and Mahomoodally, Muhammed Fettah",
year = "2018",
abstract = "The Centaurea genus has great potential in traditional systems and has attracted much interest in thedesign of novel drug formulations. The present study was focused on the chemical fingerprints andbiological properties of Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia extracts. Spec-trophotometric and LC–MS techniques were used to establish the chemical profiles of the studied extracts.Enzyme inhibitory potential was assessed against key enzymes linked to global health problems, namelyneurodegenerative diseases (acetylcholinesterase), pigmentation (tyrosinase), and diabetes ( -amylaseand  -glucosidase). The antimicrobial propensities of the extract were evaluated against 16 bacterialand fungal strains using the microdilution method. The antioxidant abilities were assessed using DPPHand ABTS radical scavenging, ferric, and cupric reducing powers, phosphomolybdenum, and ferrous metalchelation. The total phenolic compounds varied from 18.33 to 32.84 mgGAE/g extract. Total flavonoid con-tent of the extracts were in the range of 2.88–22.39 mgRE/g extract. Methanol and water extracts showedstronger antioxidant abilities compared to the ethyl acetate extracts. However, the latter extracts weremost efficient towards the target enzymes (except for tyrosinase). The water extracts also exerted con-siderable antimicrobial effects. Findings from the present work tend to support the idea that C. drabifoliasubsp. drabifolia and C. lycopifolia may be utilized as effective bio-resources for designing novel health-promoting products or ingredients. It is anticipated that results amassed from this still will open newavenues for research and contribute towards establishing primary data on these species for designingnovel phytopharmaceuticals.",
publisher = "Elsevier B.V.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia",
volume = "149",
doi = "10.1016/j.jpba.2017.11.045",
pages = "436-441"
}

Zengin, G., Zheleva-Dimitrova, D., Gevrenova, R., Nedialkov, P., Mocan, A., Ćirić, A., Glamočlija, J., Soković, M., Aktumsek, A.,& Mahomoodally, M. F.. (2018). Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier B.V.., 149, 436-441.
https://doi.org/10.1016/j.jpba.2017.11.045

Zengin G, Zheleva-Dimitrova D, Gevrenova R, Nedialkov P, Mocan A, Ćirić A, Glamočlija J, Soković M, Aktumsek A, Mahomoodally MF. Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia. in Journal of Pharmaceutical and Biomedical Analysis. 2018;149:436-441.
doi:10.1016/j.jpba.2017.11.045 .

Zengin, Gökhan, Zheleva-Dimitrova, Dimitrina, Gevrenova, Reneta, Nedialkov, Paraskev, Mocan, Andrei, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Aktumsek, Abdurrahman, Mahomoodally, Muhammed Fettah, "Identification of phenolic components via LC–MS analysis andbiological activities of two Centaurea species: C. drabifolia subsp.drabifolia and C. lycopifolia" in Journal of Pharmaceutical and Biomedical Analysis, 149 (2018):436-441,
https://doi.org/10.1016/j.jpba.2017.11.045 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Aktumsek, Abdurrahman
AU  - Ceylan, Ramazan
AU  - Uysal, Sengul
AU  - Mocan, Andrei
AU  - Guler, Gokalp Ozmen
AU  - Mahomoodally, M. Fawzi
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2017
UR  - http://xlink.rsc.org/?DOI=C6FO01847E
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2728
AB  - Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the biological and chemical profiles of different extracts (ethyl acetate, methanol and water) obtained from three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). The antioxidant (free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum), enzyme inhibitory (anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase) and antimicrobial (antibacterial and antifungal) effects were assessed to investigate their biological profiles. Moreover, the total phenolic and flavonoid contents were determined and LC-MS analysis was performed for the chemical profile of the investigated extracts. The LC-MS analysis revealed the presence of several caffeoylquinic acids in these extracts. Generally, the methanol and water extracts exhibited stronger antioxidant abilities, which correlated with the higher levels of phenolic compounds when compared to the ethyl acetate extracts. In addition, the best antimicrobial activities were obtained for the ethyl acetate and methanolic extracts. However, the ethyl acetate extract had remarkable enzyme inhibitory potential. On the basis of our results, Achillea species may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients.
T2  - Food and Function
T1  - Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia)
IS  - 3
VL  - 8
DO  - 10.1039/C6FO01847E
SP  - 1152
EP  - 1165
ER  - 

@article{
author = "Zengin, Gokhan and Aktumsek, Abdurrahman and Ceylan, Ramazan and Uysal, Sengul and Mocan, Andrei and Guler, Gokalp Ozmen and Mahomoodally, M. Fawzi and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2017",
abstract = "Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the biological and chemical profiles of different extracts (ethyl acetate, methanol and water) obtained from three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). The antioxidant (free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum), enzyme inhibitory (anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase) and antimicrobial (antibacterial and antifungal) effects were assessed to investigate their biological profiles. Moreover, the total phenolic and flavonoid contents were determined and LC-MS analysis was performed for the chemical profile of the investigated extracts. The LC-MS analysis revealed the presence of several caffeoylquinic acids in these extracts. Generally, the methanol and water extracts exhibited stronger antioxidant abilities, which correlated with the higher levels of phenolic compounds when compared to the ethyl acetate extracts. In addition, the best antimicrobial activities were obtained for the ethyl acetate and methanolic extracts. However, the ethyl acetate extract had remarkable enzyme inhibitory potential. On the basis of our results, Achillea species may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients.",
journal = "Food and Function",
title = "Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia)",
number = "3",
volume = "8",
doi = "10.1039/C6FO01847E",
pages = "1152-1165"
}

Zengin, G., Aktumsek, A., Ceylan, R., Uysal, S., Mocan, A., Guler, G. O., Mahomoodally, M. F., Glamočlija, J., Ćirić, A.,& Soković, M.. (2017). Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). in Food and Function, 8(3), 1152-1165.
https://doi.org/10.1039/C6FO01847E

Zengin G, Aktumsek A, Ceylan R, Uysal S, Mocan A, Guler GO, Mahomoodally MF, Glamočlija J, Ćirić A, Soković M. Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). in Food and Function. 2017;8(3):1152-1165.
doi:10.1039/C6FO01847E .

Zengin, Gokhan, Aktumsek, Abdurrahman, Ceylan, Ramazan, Uysal, Sengul, Mocan, Andrei, Guler, Gokalp Ozmen, Mahomoodally, M. Fawzi, Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia)" in Food and Function, 8, no. 3 (2017):1152-1165,
https://doi.org/10.1039/C6FO01847E . .