TY  - JOUR
AU  - Cavichi, Lucas Vinicius
AU  - Liberal, Ângela
AU  - Dias, Maria Inês
AU  - Mandim, Filipa
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Kalschne, Daneysa Lahis
AU  - Fernandes, Ângela
AU  - Canan, Cristiane
AU  - Barros, Lillian
AU  - Amaral, Joana S.
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/1/192
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36613411
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9818490
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5400
AB  - In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.
PB  - Basel: MDPI
T2  - Foods
T1  - Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil
IS  - 1
VL  - 12
DO  - 10.3390/foods12010192
SP  - 192
ER  - 

@article{
author = "Cavichi, Lucas Vinicius and Liberal, Ângela and Dias, Maria Inês and Mandim, Filipa and Pinela, José and Kostić, Marina and Soković, Marina and Kalschne, Daneysa Lahis and Fernandes, Ângela and Canan, Cristiane and Barros, Lillian and Amaral, Joana S.",
year = "2023",
abstract = "In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.",
publisher = "Basel: MDPI",
journal = "Foods",
title = "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil",
number = "1",
volume = "12",
doi = "10.3390/foods12010192",
pages = "192"
}

Cavichi, L. V., Liberal, Â., Dias, M. I., Mandim, F., Pinela, J., Kostić, M., Soković, M., Kalschne, D. L., Fernandes, Â., Canan, C., Barros, L.,& Amaral, J. S.. (2023). Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods
Basel: MDPI., 12(1), 192.
https://doi.org/10.3390/foods12010192

Cavichi LV, Liberal Â, Dias MI, Mandim F, Pinela J, Kostić M, Soković M, Kalschne DL, Fernandes Â, Canan C, Barros L, Amaral JS. Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods. 2023;12(1):192.
doi:10.3390/foods12010192 .

Cavichi, Lucas Vinicius, Liberal, Ângela, Dias, Maria Inês, Mandim, Filipa, Pinela, José, Kostić, Marina, Soković, Marina, Kalschne, Daneysa Lahis, Fernandes, Ângela, Canan, Cristiane, Barros, Lillian, Amaral, Joana S., "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil" in Foods, 12, no. 1 (2023):192,
https://doi.org/10.3390/foods12010192 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Chaski, Christina
AU  - Pereira, Carla
AU  - Kostić, Marina
AU  - Rouphael, Youssef
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Petropoulos, Spyridon A.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/645
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5090
AB  - The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070645
SP  - 645
ER  - 

@article{
author = "Fernandes, Ângela and Chaski, Christina and Pereira, Carla and Kostić, Marina and Rouphael, Youssef and Soković, Marina and Barros, Lillian and Petropoulos, Spyridon A.",
year = "2022",
abstract = "The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070645",
pages = "645"
}

Fernandes, Â., Chaski, C., Pereira, C., Kostić, M., Rouphael, Y., Soković, M., Barros, L.,& Petropoulos, S. A.. (2022). Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae
Basel: MDPI., 8(7), 645.
https://doi.org/10.3390/horticulturae8070645

Fernandes Â, Chaski C, Pereira C, Kostić M, Rouphael Y, Soković M, Barros L, Petropoulos SA. Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae. 2022;8(7):645.
doi:10.3390/horticulturae8070645 .

Fernandes, Ângela, Chaski, Christina, Pereira, Carla, Kostić, Marina, Rouphael, Youssef, Soković, Marina, Barros, Lillian, Petropoulos, Spyridon A., "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit" in Horticulturae, 8, no. 7 (2022):645,
https://doi.org/10.3390/horticulturae8070645 . .

TY  - JOUR
AU  - Halabura, Marisangela Isabel Wietzikoski
AU  - Avelino, Katielle Vieira
AU  - Araújo, Nelma Lopes
AU  - Kassem, Adma Soraia Serea
AU  - Seixas, Flávio Augusto Vicente
AU  - Barros, Lillian
AU  - Fernandes, Ângela
AU  - Liberal, Ângela
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Linde, Giani Andrea
AU  - Colauto, Nelson Barros
AU  - do Valle, Juliana Silveira
PY  - 2022
UR  - https://link.springer.com/10.1007/s43630-022-00344-7
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5338
AB  - The mycelial biomass of basidiomycetes is a promising source of compounds and represents an alternative for industrial and biotechnological applications. Fungi use light as information and hold photoresponse mechanisms, in which sensors respond to light wavelengths and regulate various biological processes. Therefore, this study aimed to investigate the effects of blue, green, and red lights on the growth, chemical composition, and antioxidant and antimicrobial activity of Lentinus crinitus mycelial biomass. The chemical composition of the mycelial biomass was determined by chromatographic methods, antioxidant activity was analyzed by in vitro assays, and antimicrobial activity was investigated by the microdilution assay. The highest mycelial biomass yield was observed under blue-light cultivation. Many primordia arose under blue or green light, whereas the stroma was formed under red light. The presence of light altered the primary fungal metabolism, increasing the carbohydrate, tocopherol, fatty acid, and soluble sugar contents, mostly mannitol, and reducing the protein and organic acid concentrations. Cultivation under red light increased the phenol concentration. In contrast, cultivation under blue and green lights decreased phenol concentration. Benzoic and gallic acids were the main phenolic acids in the hydroalcoholic extracts, and the latter acids increased in all cultures under light, especially red light. Mycelial biomass cultivated under red light showed the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ferric reducing antioxidant power (FRAP) method showed that all light wavelengths increased the antioxidant activity of mycelial biomass, with the highest value under red light. Moreover, the beta-carotene/linoleic acid co-oxidation (BCLA) assay demonstrated that the antioxidant activity was affected by light cultivation. Mycelial biomass grown under all conditions exhibited antibacterial and antifungal activities. Thus, mycelial biomass cultivation of L. crinitus under light conditions may be a promising strategy for controlling the mycelial chemical composition and biomass yield.
PB  - London: Springer Nature
T2  - Photochemical & Photobiological Sciences
T1  - Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass
DO  - 10.1007/s43630-022-00344-7
ER  - 

@article{
author = "Halabura, Marisangela Isabel Wietzikoski and Avelino, Katielle Vieira and Araújo, Nelma Lopes and Kassem, Adma Soraia Serea and Seixas, Flávio Augusto Vicente and Barros, Lillian and Fernandes, Ângela and Liberal, Ângela and Ivanov, Marija and Soković, Marina and Linde, Giani Andrea and Colauto, Nelson Barros and do Valle, Juliana Silveira",
year = "2022",
abstract = "The mycelial biomass of basidiomycetes is a promising source of compounds and represents an alternative for industrial and biotechnological applications. Fungi use light as information and hold photoresponse mechanisms, in which sensors respond to light wavelengths and regulate various biological processes. Therefore, this study aimed to investigate the effects of blue, green, and red lights on the growth, chemical composition, and antioxidant and antimicrobial activity of Lentinus crinitus mycelial biomass. The chemical composition of the mycelial biomass was determined by chromatographic methods, antioxidant activity was analyzed by in vitro assays, and antimicrobial activity was investigated by the microdilution assay. The highest mycelial biomass yield was observed under blue-light cultivation. Many primordia arose under blue or green light, whereas the stroma was formed under red light. The presence of light altered the primary fungal metabolism, increasing the carbohydrate, tocopherol, fatty acid, and soluble sugar contents, mostly mannitol, and reducing the protein and organic acid concentrations. Cultivation under red light increased the phenol concentration. In contrast, cultivation under blue and green lights decreased phenol concentration. Benzoic and gallic acids were the main phenolic acids in the hydroalcoholic extracts, and the latter acids increased in all cultures under light, especially red light. Mycelial biomass cultivated under red light showed the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ferric reducing antioxidant power (FRAP) method showed that all light wavelengths increased the antioxidant activity of mycelial biomass, with the highest value under red light. Moreover, the beta-carotene/linoleic acid co-oxidation (BCLA) assay demonstrated that the antioxidant activity was affected by light cultivation. Mycelial biomass grown under all conditions exhibited antibacterial and antifungal activities. Thus, mycelial biomass cultivation of L. crinitus under light conditions may be a promising strategy for controlling the mycelial chemical composition and biomass yield.",
publisher = "London: Springer Nature",
journal = "Photochemical & Photobiological Sciences",
title = "Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass",
doi = "10.1007/s43630-022-00344-7"
}

Halabura, M. I. W., Avelino, K. V., Araújo, N. L., Kassem, A. S. S., Seixas, F. A. V., Barros, L., Fernandes, Â., Liberal, Â., Ivanov, M., Soković, M., Linde, G. A., Colauto, N. B.,& do Valle, J. S.. (2022). Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass. in Photochemical & Photobiological Sciences
London: Springer Nature..
https://doi.org/10.1007/s43630-022-00344-7

Halabura MIW, Avelino KV, Araújo NL, Kassem ASS, Seixas FAV, Barros L, Fernandes Â, Liberal Â, Ivanov M, Soković M, Linde GA, Colauto NB, do Valle JS. Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass. in Photochemical & Photobiological Sciences. 2022;.
doi:10.1007/s43630-022-00344-7 .

Halabura, Marisangela Isabel Wietzikoski, Avelino, Katielle Vieira, Araújo, Nelma Lopes, Kassem, Adma Soraia Serea, Seixas, Flávio Augusto Vicente, Barros, Lillian, Fernandes, Ângela, Liberal, Ângela, Ivanov, Marija, Soković, Marina, Linde, Giani Andrea, Colauto, Nelson Barros, do Valle, Juliana Silveira, "Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass" in Photochemical & Photobiological Sciences (2022),
https://doi.org/10.1007/s43630-022-00344-7 . .

TY  - JOUR
AU  - Kissanga, Raquel
AU  - Liberal, Ângela
AU  - Diniz, Inês
AU  - Rodrigues, Ana S. B.
AU  - Baptista-Ferreira, João L.
AU  - Batista, Dora
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Fernandes, Ângela
AU  - Barros, Lillian
AU  - Catarino, Luís
PY  - 2022
UR  - https://www.mdpi.com/2304-8158/11/20/3240
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5179
AB  - The harvesting, processing, and sale of wild edible mushrooms (WEM) is a relevant economic activity in Angola and a good example of the use of non-wood forest products for food. Although there is deep traditional knowledge about the general properties of WEMs, a huge gap remains in detailed scientific knowledge. Thus, this study aimed to investigate the socio-economic importance of the species sold at local markets in Huila, Angola, from their molecular identification to the assessment of their nutritional, chemical, and bioactive profiles. From the eight WEM morphotypes studied, five were identified based on phenotypical and molecular approaches (four Russula spp., and Amanita loosei). The studied mushrooms proved to be a rich source of carbohydrates, proteins, and ashes, also presenting low amounts of fat. Chemical analyses further revealed mannitol as the main free sugar in all samples, and organic acids, namely, oxalic, quinic, malic, citric, and fumaric acids in low amounts. Additionally, the α-tocopherol isoform and monounsaturated fatty acids were predominant. Regarding phenolic acids, protocatechuic, p-hydroxybenzoic, p-coumaric, and cinnamic acids were detected in all mushroom hydroethanolic extracts, being responsible for their antioxidant, antibacterial, and antifungal activities. Our investigation contributes to the identification and knowledge of WEMs as important complementary food sources in Angola, some of which were reported for the first time, promoting their utilization as a basis of nutritional and functional ingredients, as being able to be part of a balanced diet and to be used in new bio-based formulations.
PB  - Basel: MDPI
T2  - Foods
T1  - Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola
IS  - 20
VL  - 11
DO  - 10.3390/foods11203240
SP  - 3240
ER  - 

@article{
author = "Kissanga, Raquel and Liberal, Ângela and Diniz, Inês and Rodrigues, Ana S. B. and Baptista-Ferreira, João L. and Batista, Dora and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Fernandes, Ângela and Barros, Lillian and Catarino, Luís",
year = "2022",
abstract = "The harvesting, processing, and sale of wild edible mushrooms (WEM) is a relevant economic activity in Angola and a good example of the use of non-wood forest products for food. Although there is deep traditional knowledge about the general properties of WEMs, a huge gap remains in detailed scientific knowledge. Thus, this study aimed to investigate the socio-economic importance of the species sold at local markets in Huila, Angola, from their molecular identification to the assessment of their nutritional, chemical, and bioactive profiles. From the eight WEM morphotypes studied, five were identified based on phenotypical and molecular approaches (four Russula spp., and Amanita loosei). The studied mushrooms proved to be a rich source of carbohydrates, proteins, and ashes, also presenting low amounts of fat. Chemical analyses further revealed mannitol as the main free sugar in all samples, and organic acids, namely, oxalic, quinic, malic, citric, and fumaric acids in low amounts. Additionally, the α-tocopherol isoform and monounsaturated fatty acids were predominant. Regarding phenolic acids, protocatechuic, p-hydroxybenzoic, p-coumaric, and cinnamic acids were detected in all mushroom hydroethanolic extracts, being responsible for their antioxidant, antibacterial, and antifungal activities. Our investigation contributes to the identification and knowledge of WEMs as important complementary food sources in Angola, some of which were reported for the first time, promoting their utilization as a basis of nutritional and functional ingredients, as being able to be part of a balanced diet and to be used in new bio-based formulations.",
publisher = "Basel: MDPI",
journal = "Foods",
title = "Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola",
number = "20",
volume = "11",
doi = "10.3390/foods11203240",
pages = "3240"
}

Kissanga, R., Liberal, Â., Diniz, I., Rodrigues, A. S. B., Baptista-Ferreira, J. L., Batista, D., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Fernandes, Â., Barros, L.,& Catarino, L.. (2022). Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola. in Foods
Basel: MDPI., 11(20), 3240.
https://doi.org/10.3390/foods11203240

Kissanga R, Liberal Â, Diniz I, Rodrigues ASB, Baptista-Ferreira JL, Batista D, Ivanov M, Soković M, Ferreira ICFR, Fernandes Â, Barros L, Catarino L. Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola. in Foods. 2022;11(20):3240.
doi:10.3390/foods11203240 .

Kissanga, Raquel, Liberal, Ângela, Diniz, Inês, Rodrigues, Ana S. B., Baptista-Ferreira, João L., Batista, Dora, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Fernandes, Ângela, Barros, Lillian, Catarino, Luís, "Biochemical and Molecular Profiling of Wild Edible Mushrooms from Huila, Angola" in Foods, 11, no. 20 (2022):3240,
https://doi.org/10.3390/foods11203240 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Finimundy, Tiane C.
AU  - Polyzos, Nikolaos
AU  - Pinela, José
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/639
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5119
AB  - In the present study, the chemical profile and bioactive properties of the roots of turnip-rooted parsley (Petroselinum crispum spp. tuberosum) germplasm were evaluated. For this purpose, plants from seventeen parsley cultivars were grown in 6 L pots, and the obtained roots were analyzed in terms of nutritional value, chemical composition (tocopherols, sugars and organic and fatty acids) and bioactive content (antioxidant activity, phenolic compound composition and antimicrobial properties). Our results showed great variability in terms of the chemical composition and bioactive properties of root parsley germplasm. A higher fresh root yield was recorded for the common “Root parsley” common variety (164 g/pot), followed by the varieties “Osborne” (109 g/pot), “Sonata” (104 g/pot), “Kaśka” (104 g/pot) and “Halblange Berlinska” (103 g/pot), whereas the lowest yield was recorded for the “Hanacka” variety (69 g/pot). A significant variation was also observed in the nutritional value parameters: the roots of the “Sonata” genotype showed the highest fat content; “Arat”, “Osborne” and “Olomuńcka” had the highest ash content; the “Alba” cultivar contained significantly higher amounts of carbohydrates; and the “Vistula” cultivar showed the highest energetic value. The only detected isoforms of vitamin E were α- and δ-tocopherols; content varied depending on the cultivar, although α-tocopherol was the most abundant compound in most cultivars, especially in the “Arat” cultivar. Sucrose was the most abundant free sugar detected, especially in the “Sonata” cultivar (16.96 g/100 g dw), followed by apiose (2.93–5.55 g/100 g dw), glucose (1.3–3.47 g/100 g dw) and fructose (1.37–3.03 g/100 g dw). Moreover, malic acid was the most abundant organic acid in most of the tested cultivars. Twenty-one individual fatty acids were identified in all the studied cultivars, with linoleic (47.9–57.1%) and palmitic acid (20.66–20.5%) being the most abundant. Nineteen individual phenolic compounds were tentatively identified, including three phenolic acids, fourteen flavonoids and two hydrolyzable tannins, while apigenin-O-pentoside-O-hexoside was the most abundant. The antioxidant activity differed between the tested assays (TBARS and OxHLIA), and the most effective cultivars for the TBARS assay (“Root parsley (common variety)” and “Berlinski Halblange Springer”) were those with the lowest antioxidant activity for the OxHLIA assay after 120 min. Finally, in most cases, the root extracts were more efficient or similarly effective compared to the positive controls against the tested bacteria and fungi. In conclusion, our results provide information regarding the chemical characterization and the bioactivities of the roots of turnip-rooted parsley germplasm that could be further exploited in sustainable and diversified agro-ecosystems through the introduction of this species as a novel/complementary crop in the traditional farming systems of the Mediterranean basin.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070639
SP  - 639
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Finimundy, Tiane C. and Polyzos, Nikolaos and Pinela, José and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2022",
abstract = "In the present study, the chemical profile and bioactive properties of the roots of turnip-rooted parsley (Petroselinum crispum spp. tuberosum) germplasm were evaluated. For this purpose, plants from seventeen parsley cultivars were grown in 6 L pots, and the obtained roots were analyzed in terms of nutritional value, chemical composition (tocopherols, sugars and organic and fatty acids) and bioactive content (antioxidant activity, phenolic compound composition and antimicrobial properties). Our results showed great variability in terms of the chemical composition and bioactive properties of root parsley germplasm. A higher fresh root yield was recorded for the common “Root parsley” common variety (164 g/pot), followed by the varieties “Osborne” (109 g/pot), “Sonata” (104 g/pot), “Kaśka” (104 g/pot) and “Halblange Berlinska” (103 g/pot), whereas the lowest yield was recorded for the “Hanacka” variety (69 g/pot). A significant variation was also observed in the nutritional value parameters: the roots of the “Sonata” genotype showed the highest fat content; “Arat”, “Osborne” and “Olomuńcka” had the highest ash content; the “Alba” cultivar contained significantly higher amounts of carbohydrates; and the “Vistula” cultivar showed the highest energetic value. The only detected isoforms of vitamin E were α- and δ-tocopherols; content varied depending on the cultivar, although α-tocopherol was the most abundant compound in most cultivars, especially in the “Arat” cultivar. Sucrose was the most abundant free sugar detected, especially in the “Sonata” cultivar (16.96 g/100 g dw), followed by apiose (2.93–5.55 g/100 g dw), glucose (1.3–3.47 g/100 g dw) and fructose (1.37–3.03 g/100 g dw). Moreover, malic acid was the most abundant organic acid in most of the tested cultivars. Twenty-one individual fatty acids were identified in all the studied cultivars, with linoleic (47.9–57.1%) and palmitic acid (20.66–20.5%) being the most abundant. Nineteen individual phenolic compounds were tentatively identified, including three phenolic acids, fourteen flavonoids and two hydrolyzable tannins, while apigenin-O-pentoside-O-hexoside was the most abundant. The antioxidant activity differed between the tested assays (TBARS and OxHLIA), and the most effective cultivars for the TBARS assay (“Root parsley (common variety)” and “Berlinski Halblange Springer”) were those with the lowest antioxidant activity for the OxHLIA assay after 120 min. Finally, in most cases, the root extracts were more efficient or similarly effective compared to the positive controls against the tested bacteria and fungi. In conclusion, our results provide information regarding the chemical characterization and the bioactivities of the roots of turnip-rooted parsley germplasm that could be further exploited in sustainable and diversified agro-ecosystems through the introduction of this species as a novel/complementary crop in the traditional farming systems of the Mediterranean basin.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070639",
pages = "639"
}

Petropoulos, S. A., Fernandes, Â., Finimundy, T. C., Polyzos, N., Pinela, J., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2022). The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm. in Horticulturae
Basel: MDPI., 8(7), 639.
https://doi.org/10.3390/horticulturae8070639

Petropoulos SA, Fernandes Â, Finimundy TC, Polyzos N, Pinela J, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm. in Horticulturae. 2022;8(7):639.
doi:10.3390/horticulturae8070639 .

Petropoulos, Spyridon A., Fernandes, Ângela, Finimundy, Tiane C., Polyzos, Nikolaos, Pinela, José, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm" in Horticulturae, 8, no. 7 (2022):639,
https://doi.org/10.3390/horticulturae8070639 . .

TY  - JOUR
AU  - Mocan, Andrei
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Gavrilaş, Laura
AU  - Ferreira, Isabel C. F. R.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Babotă, Mihai
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.881210/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9168797
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5006
AB  - The members of Cystus genus are perenial shrubs with a well-established use in traditional medicine. Among these, C. creticus is the most popular, herbal preparations obtained from its aerial parts being recognized as antimicrobial, antitumor and anti-inflammatory agents. The present study aimed to evaluate phytochemical profile and bioactive potential of aqueous and hydroethanolic extracts of C. creticus aerial parts harvested from two different areas of Rhodes island. LC-DAD-ESI/MSn analysis revealed the presence of myricetin and quercetin glycosides as main compounds, especially in aqueous extracts, being probably responsible for their enhanced antioxidant and antimicrobial potential. On the other side, hydroethanolic preparations exerted a strong anti-inflammatory and anti-biofilm activity. Our findings suggest that the use of solvents with intermediate polarity can assure the best recovery of bioactive compounds from C. creticus, increasing the extraction yield for other non-phenolic compounds which can enhance therapeutic potential of the extract through a synergistic action.
T2  - Frontiers in Nutrition
T1  - Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.
VL  - 9
DO  - 10.3389/fnut.2022.881210
SP  - 881210
ER  - 

@article{
author = "Mocan, Andrei and Fernandes, Ângela and Calhelha, Ricardo C. and Gavrilaş, Laura and Ferreira, Isabel C. F. R. and Ivanov, Marija and Soković, Marina and Barros, Lillian and Babotă, Mihai",
year = "2022",
abstract = "The members of Cystus genus are perenial shrubs with a well-established use in traditional medicine. Among these, C. creticus is the most popular, herbal preparations obtained from its aerial parts being recognized as antimicrobial, antitumor and anti-inflammatory agents. The present study aimed to evaluate phytochemical profile and bioactive potential of aqueous and hydroethanolic extracts of C. creticus aerial parts harvested from two different areas of Rhodes island. LC-DAD-ESI/MSn analysis revealed the presence of myricetin and quercetin glycosides as main compounds, especially in aqueous extracts, being probably responsible for their enhanced antioxidant and antimicrobial potential. On the other side, hydroethanolic preparations exerted a strong anti-inflammatory and anti-biofilm activity. Our findings suggest that the use of solvents with intermediate polarity can assure the best recovery of bioactive compounds from C. creticus, increasing the extraction yield for other non-phenolic compounds which can enhance therapeutic potential of the extract through a synergistic action.",
journal = "Frontiers in Nutrition",
title = "Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.",
volume = "9",
doi = "10.3389/fnut.2022.881210",
pages = "881210"
}

Mocan, A., Fernandes, Â., Calhelha, R. C., Gavrilaş, L., Ferreira, I. C. F. R., Ivanov, M., Soković, M., Barros, L.,& Babotă, M.. (2022). Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.. in Frontiers in Nutrition, 9, 881210.
https://doi.org/10.3389/fnut.2022.881210

Mocan A, Fernandes Â, Calhelha RC, Gavrilaş L, Ferreira ICFR, Ivanov M, Soković M, Barros L, Babotă M. Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island.. in Frontiers in Nutrition. 2022;9:881210.
doi:10.3389/fnut.2022.881210 .

Mocan, Andrei, Fernandes, Ângela, Calhelha, Ricardo C., Gavrilaş, Laura, Ferreira, Isabel C. F. R., Ivanov, Marija, Soković, Marina, Barros, Lillian, Babotă, Mihai, "Bioactive Compounds and Functional Properties of Herbal Preparations of Cystus creticus L. Collected From Rhodes Island." in Frontiers in Nutrition, 9 (2022):881210,
https://doi.org/10.3389/fnut.2022.881210 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2073-4395/11/3/576
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4419
AB  - The Mediterranean basin is abundant in wild edible species with numerous health beneficial effects due to the presence of various bioactive phytochemicals. In the present work, the effect of nitrogen fertilization rates (0 ppm, (N0), 200 ppm (N1), 400 ppm (N2), and 600 ppm (N3) of total N) and growth substrate composition (soil or peat/perlite (2/1; v/v)) on the chemical composition and bioactive properties of Centaurea raphanina ssp. mixta plants was evaluated. The results of the study showed that both the tested factors affected nutritional value of the edible leaves, with the soil × N1 treatment being the most beneficial for fat, protein, and carbohydrate content and energetic value. On the other hand, the peat/perlite-grown plants that received 200 ppm of N had the highest content in α-, γ-, and total tocopherols, while the control treatment of soil-grown plants was the richest in individual and total sugars. Oxalic, citric, and total organic acids were the highest in the N2 × soil treatment, while malic acid was the highest in control treatment of the same substrate. The main fatty acids were palmitic, α-linolenic, and linoleic acids, with the highest contents being observed in the N0 × soil, N3 × soil, and N3 × peat/perlite treatments, respectively. The major phenolic compounds were pinocembrim neohesperidoside and pinocembrim acetyl neohesperidoside isomer II, with the highest content being observed in the N1 × soil treatment. The highest antihemolytic activity was observed in the N3 × peat/perlite treatment, while the most effective treatments against lipid peroxidation were N0 (in both soil and peat/perlite combinations) and N1 × peat/perlite. Lastly, all the tested extracts (except for N1 × soil) showed promising cytotoxic effects against HeLa (cervical carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), and NCI-H460 (non-small-cell lung cancer), while all the tested extracts exhibited better antifungal activities (lower minimal inhibition concentration (MIC) values) against Trichoderma viride than the positive controls. Overall, the present results suggest that the application of cost-effective practices such as the nitrogen application and the selection of growth substrate may regulate the chemical composition and the bioactive properties of C. raphanina ssp. mixta species and increase its added value under commercial cultivation conditions.
T2  - Agronomy
T1  - Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark
IS  - 3
VL  - 11
DO  - 10.3390/agronomy11030576
SP  - 576
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "The Mediterranean basin is abundant in wild edible species with numerous health beneficial effects due to the presence of various bioactive phytochemicals. In the present work, the effect of nitrogen fertilization rates (0 ppm, (N0), 200 ppm (N1), 400 ppm (N2), and 600 ppm (N3) of total N) and growth substrate composition (soil or peat/perlite (2/1; v/v)) on the chemical composition and bioactive properties of Centaurea raphanina ssp. mixta plants was evaluated. The results of the study showed that both the tested factors affected nutritional value of the edible leaves, with the soil × N1 treatment being the most beneficial for fat, protein, and carbohydrate content and energetic value. On the other hand, the peat/perlite-grown plants that received 200 ppm of N had the highest content in α-, γ-, and total tocopherols, while the control treatment of soil-grown plants was the richest in individual and total sugars. Oxalic, citric, and total organic acids were the highest in the N2 × soil treatment, while malic acid was the highest in control treatment of the same substrate. The main fatty acids were palmitic, α-linolenic, and linoleic acids, with the highest contents being observed in the N0 × soil, N3 × soil, and N3 × peat/perlite treatments, respectively. The major phenolic compounds were pinocembrim neohesperidoside and pinocembrim acetyl neohesperidoside isomer II, with the highest content being observed in the N1 × soil treatment. The highest antihemolytic activity was observed in the N3 × peat/perlite treatment, while the most effective treatments against lipid peroxidation were N0 (in both soil and peat/perlite combinations) and N1 × peat/perlite. Lastly, all the tested extracts (except for N1 × soil) showed promising cytotoxic effects against HeLa (cervical carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), and NCI-H460 (non-small-cell lung cancer), while all the tested extracts exhibited better antifungal activities (lower minimal inhibition concentration (MIC) values) against Trichoderma viride than the positive controls. Overall, the present results suggest that the application of cost-effective practices such as the nitrogen application and the selection of growth substrate may regulate the chemical composition and the bioactive properties of C. raphanina ssp. mixta species and increase its added value under commercial cultivation conditions.",
journal = "Agronomy",
title = "Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark",
number = "3",
volume = "11",
doi = "10.3390/agronomy11030576",
pages = "576"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark. in Agronomy, 11(3), 576.
https://doi.org/10.3390/agronomy11030576

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark. in Agronomy. 2021;11(3):576.
doi:10.3390/agronomy11030576 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark" in Agronomy, 11, no. 3 (2021):576,
https://doi.org/10.3390/agronomy11030576 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Bancessi, Aducabe
AU  - Pinela, José
AU  - Inês Dias, Maria
AU  - Liberal, Ângela
AU  - Calhelha, Ricardo C.
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - CATARINO, Luís
AU  - C.F.R. Ferreira, Isabel
AU  - Barros, Lillian
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33038772
UR  - https://radar.ibiss.bg.ac.rs/123456789/3913
AB  - Moringa oleifera is an edible medicinal plant used to fight malnutrition in Africa. In this study, M. oleifera flowers, fruits and seeds from Guinea-Bissau were characterized for their nutritional composition and hydroethanolic and aqueous extracts were prepared to investigate the phenolic profiles and bioactivities. Seeds presented higher levels of proteins (~31 g/100 g dw), fat (~26 g/100 g dw) and flavan-3-ol derivatives, while carbohydrates, proteins, citric acid, and glycosylated flavonoids were abundant in fruits and flowers, these last samples also being rich in α-tocopherol (~18 mg/100 g dw). Some of the identified polyphenols had never been described in M. oleifera. In general, hydroethanolic extracts contained more polyphenols and were more active against lipid peroxidation, NO production, and tumour cells growth. Significant antimicrobial effects against the tested bacteria and fungi strains were displayed by both hydroethanolic and aqueous extracts. The M. oleifera potential to fight malnutrition and health issues was highlighted.
PB  - Elsevier BV
T2  - Food Chemistry
T1  - Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa)
IS  - Pt 1
VL  - 341
DO  - 10.1016/j.foodchem.2020.128229
SP  - 128229
ER  - 

@article{
author = "Fernandes, Ângela and Bancessi, Aducabe and Pinela, José and Inês Dias, Maria and Liberal, Ângela and Calhelha, Ricardo C. and Ćirić, Ana and Soković, Marina and CATARINO, Luís and C.F.R. Ferreira, Isabel and Barros, Lillian",
year = "2021",
abstract = "Moringa oleifera is an edible medicinal plant used to fight malnutrition in Africa. In this study, M. oleifera flowers, fruits and seeds from Guinea-Bissau were characterized for their nutritional composition and hydroethanolic and aqueous extracts were prepared to investigate the phenolic profiles and bioactivities. Seeds presented higher levels of proteins (~31 g/100 g dw), fat (~26 g/100 g dw) and flavan-3-ol derivatives, while carbohydrates, proteins, citric acid, and glycosylated flavonoids were abundant in fruits and flowers, these last samples also being rich in α-tocopherol (~18 mg/100 g dw). Some of the identified polyphenols had never been described in M. oleifera. In general, hydroethanolic extracts contained more polyphenols and were more active against lipid peroxidation, NO production, and tumour cells growth. Significant antimicrobial effects against the tested bacteria and fungi strains were displayed by both hydroethanolic and aqueous extracts. The M. oleifera potential to fight malnutrition and health issues was highlighted.",
publisher = "Elsevier BV",
journal = "Food Chemistry",
title = "Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa)",
number = "Pt 1",
volume = "341",
doi = "10.1016/j.foodchem.2020.128229",
pages = "128229"
}

Fernandes, Â., Bancessi, A., Pinela, J., Inês Dias, M., Liberal, Â., Calhelha, R. C., Ćirić, A., Soković, M., CATARINO, L., C.F.R. Ferreira, I.,& Barros, L.. (2021). Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa). in Food Chemistry
Elsevier BV., 341(Pt 1), 128229.
https://doi.org/10.1016/j.foodchem.2020.128229

Fernandes Â, Bancessi A, Pinela J, Inês Dias M, Liberal Â, Calhelha RC, Ćirić A, Soković M, CATARINO L, C.F.R. Ferreira I, Barros L. Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa). in Food Chemistry. 2021;341(Pt 1):128229.
doi:10.1016/j.foodchem.2020.128229 .

Fernandes, Ângela, Bancessi, Aducabe, Pinela, José, Inês Dias, Maria, Liberal, Ângela, Calhelha, Ricardo C., Ćirić, Ana, Soković, Marina, CATARINO, Luís, C.F.R. Ferreira, Isabel, Barros, Lillian, "Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa)" in Food Chemistry, 341, no. Pt 1 (2021):128229,
https://doi.org/10.1016/j.foodchem.2020.128229 . .

TY  - JOUR
AU  - Cardoso, Rossana V. C.
AU  - Carocho, Márcio
AU  - Fernandes, Ângela
AU  - Pinela, José
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Zied, Diego Cunha
AU  - Cobos, Juan Diego Valenzuela
AU  - González-Paramás, Ana M.
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2071-1050/13/9/5019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4241
AB  - Supplementation of the substrate of mushrooms with calcium silicate and other minerals is usually used as a preventive measure against pests and other contaminants during the production of oyster mushrooms. Little is known of the effects of this supplementation on the quality of the mushrooms produced. In the work described herein, the supplementation of oyster mushrooms was performed with 5 supplementation levels (0%, 0.5%, 1%, 2% and 4%) on mushrooms from two different locations in Brazil, the two flushes of mushrooms produced were analysed in terms of phenolic compounds, organic acids, and the antioxidant, antibacterial and antifungal activities, and finally the data was subjected to a linear discriminant analysis to understand the discrimination of the supplementation percentages. Overall, intermediate supplementation until 1% seemed to have a positive effect on the mushrooms from Mogi-das-Cruzes region, while high supplementation favoured the mushrooms from the region of Presidente Prudente. Supplementation showed positive effects on the mushrooms by increasing the production of some secondary metabolites while not showing any negative cytotoxic effects.
PB  - MDPI AG
T2  - Sustainability
T1  - Antioxidant and Antimicrobial Influence on Oyster Mushrooms (Pleurotus ostreatus) from Substrate Supplementation of Calcium Silicate
IS  - 9
VL  - 13
DO  - 10.3390/su13095019
SP  - 5019
ER  - 

@article{
author = "Cardoso, Rossana V. C. and Carocho, Márcio and Fernandes, Ângela and Pinela, José and Stojković, Dejan and Soković, Marina and Zied, Diego Cunha and Cobos, Juan Diego Valenzuela and González-Paramás, Ana M. and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Supplementation of the substrate of mushrooms with calcium silicate and other minerals is usually used as a preventive measure against pests and other contaminants during the production of oyster mushrooms. Little is known of the effects of this supplementation on the quality of the mushrooms produced. In the work described herein, the supplementation of oyster mushrooms was performed with 5 supplementation levels (0%, 0.5%, 1%, 2% and 4%) on mushrooms from two different locations in Brazil, the two flushes of mushrooms produced were analysed in terms of phenolic compounds, organic acids, and the antioxidant, antibacterial and antifungal activities, and finally the data was subjected to a linear discriminant analysis to understand the discrimination of the supplementation percentages. Overall, intermediate supplementation until 1% seemed to have a positive effect on the mushrooms from Mogi-das-Cruzes region, while high supplementation favoured the mushrooms from the region of Presidente Prudente. Supplementation showed positive effects on the mushrooms by increasing the production of some secondary metabolites while not showing any negative cytotoxic effects.",
publisher = "MDPI AG",
journal = "Sustainability",
title = "Antioxidant and Antimicrobial Influence on Oyster Mushrooms (Pleurotus ostreatus) from Substrate Supplementation of Calcium Silicate",
number = "9",
volume = "13",
doi = "10.3390/su13095019",
pages = "5019"
}

Cardoso, R. V. C., Carocho, M., Fernandes, Â., Pinela, J., Stojković, D., Soković, M., Zied, D. C., Cobos, J. D. V., González-Paramás, A. M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Antioxidant and Antimicrobial Influence on Oyster Mushrooms (Pleurotus ostreatus) from Substrate Supplementation of Calcium Silicate. in Sustainability
MDPI AG., 13(9), 5019.
https://doi.org/10.3390/su13095019

Cardoso RVC, Carocho M, Fernandes Â, Pinela J, Stojković D, Soković M, Zied DC, Cobos JDV, González-Paramás AM, Ferreira ICFR, Barros L. Antioxidant and Antimicrobial Influence on Oyster Mushrooms (Pleurotus ostreatus) from Substrate Supplementation of Calcium Silicate. in Sustainability. 2021;13(9):5019.
doi:10.3390/su13095019 .

Cardoso, Rossana V. C., Carocho, Márcio, Fernandes, Ângela, Pinela, José, Stojković, Dejan, Soković, Marina, Zied, Diego Cunha, Cobos, Juan Diego Valenzuela, González-Paramás, Ana M., Ferreira, Isabel C. F. R., Barros, Lillian, "Antioxidant and Antimicrobial Influence on Oyster Mushrooms (Pleurotus ostreatus) from Substrate Supplementation of Calcium Silicate" in Sustainability, 13, no. 9 (2021):5019,
https://doi.org/10.3390/su13095019 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Figueiredo, Sara
AU  - Finimundy, Tiane C.
AU  - Pinela, José
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Petropoulos, Spyridon A.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2071-1050/13/12/6869
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4418
AB  - Biostimulants are a novel and eco-friendly agronomic tool with practical applications in alleviating negative effects of environmental stressors. The present work studied the effects of three biostimulant products (Nomoren (N), Twin-Antistress (TW), and X-Stress (XS)) under normal irrigation (W+) and water deficit irrigation conditions (W−) on the nutritional, chemical composition and bioactive properties of common bean fresh pods. A variable effect of biostimulants and water deficit irrigation was observed on nutritional value parameters, while fructose and sucrose were the main detected sugars, especially in NW+ and CW− treatments. Oxalic, malic, and citric acid were the main detected organic acids, while γ- and total tocopherol content was the highest in TWW+. (+)-Catechin and (−)-epicatechin were the most abundant phenolic compounds, especially in the NW− treatment. A variable antioxidant capacity was observed for the Thiobarbituric Acid Reactive Substances (TBARS) and Oxidative Haemolysis assays (OxHLIA), while TWW+ extracts showed the best overall results against the tested fungi. In conclusion, the tested biostimulants had a positive effect on chemical composition and bioactivities of purple bean depending on the irrigation regime.
T2  - Sustainability
T1  - Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application
IS  - 12
VL  - 13
DO  - 10.3390/su13126869
SP  - 6869
ER  - 

@article{
author = "Fernandes, Ângela and Figueiredo, Sara and Finimundy, Tiane C. and Pinela, José and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Petropoulos, Spyridon A. and Barros, Lillian",
year = "2021",
abstract = "Biostimulants are a novel and eco-friendly agronomic tool with practical applications in alleviating negative effects of environmental stressors. The present work studied the effects of three biostimulant products (Nomoren (N), Twin-Antistress (TW), and X-Stress (XS)) under normal irrigation (W+) and water deficit irrigation conditions (W−) on the nutritional, chemical composition and bioactive properties of common bean fresh pods. A variable effect of biostimulants and water deficit irrigation was observed on nutritional value parameters, while fructose and sucrose were the main detected sugars, especially in NW+ and CW− treatments. Oxalic, malic, and citric acid were the main detected organic acids, while γ- and total tocopherol content was the highest in TWW+. (+)-Catechin and (−)-epicatechin were the most abundant phenolic compounds, especially in the NW− treatment. A variable antioxidant capacity was observed for the Thiobarbituric Acid Reactive Substances (TBARS) and Oxidative Haemolysis assays (OxHLIA), while TWW+ extracts showed the best overall results against the tested fungi. In conclusion, the tested biostimulants had a positive effect on chemical composition and bioactivities of purple bean depending on the irrigation regime.",
journal = "Sustainability",
title = "Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application",
number = "12",
volume = "13",
doi = "10.3390/su13126869",
pages = "6869"
}

Fernandes, Â., Figueiredo, S., Finimundy, T. C., Pinela, J., Tzortzakis, N., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Petropoulos, S. A.,& Barros, L.. (2021). Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application. in Sustainability, 13(12), 6869.
https://doi.org/10.3390/su13126869

Fernandes Â, Figueiredo S, Finimundy TC, Pinela J, Tzortzakis N, Ivanov M, Soković M, Ferreira ICFR, Petropoulos SA, Barros L. Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application. in Sustainability. 2021;13(12):6869.
doi:10.3390/su13126869 .

Fernandes, Ângela, Figueiredo, Sara, Finimundy, Tiane C., Pinela, José, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Petropoulos, Spyridon A., Barros, Lillian, "Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application" in Sustainability, 13, no. 12 (2021):6869,
https://doi.org/10.3390/su13126869 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Liberal, Ângela
AU  - Pinela, José
AU  - Finimundy, Tiane C.
AU  - Bancessi, Aducabe
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Catarino, Luís
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2212429221004259
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4462
AB  - Moringa oleifera Lam. is a nutraceutical edible plant used to fight malnutrition in developing countries. This investigation describes the nutritional, chemical and bioactive assets of wild and commercially acquired M. oleifera leaves from Guinea-Bissau. Both samples presented significant differences in the contents of almost all the analysed parameters. Carbohydrates and proteins were the major constituents of the leaves, which also showed an interesting profile of tocopherols, organic acids, unsaturated fatty acids, and phenolic compounds. The infused extract of the commercial leaf sample was more effective than other preparations against lipid peroxidation and oxidative haemolysis. In turn, higher antibacterial activity was achieved with hydroethanolic and infused extracts of the wild sample, which extracts also displayed superior antifungal activity. Overall, the commercially acquired M. oleifera leaves stand out with better quality profiles than the wild collected, which aroused interest in studying the processing methods used locally to process this functional food.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau
VL  - 43
DO  - 10.1016/j.fbio.2021.101300
SP  - 101300
ER  - 

@article{
author = "Fernandes, Ângela and Liberal, Ângela and Pinela, José and Finimundy, Tiane C. and Bancessi, Aducabe and Ćirić, Ana and Soković, Marina and Catarino, Luís and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2021",
abstract = "Moringa oleifera Lam. is a nutraceutical edible plant used to fight malnutrition in developing countries. This investigation describes the nutritional, chemical and bioactive assets of wild and commercially acquired M. oleifera leaves from Guinea-Bissau. Both samples presented significant differences in the contents of almost all the analysed parameters. Carbohydrates and proteins were the major constituents of the leaves, which also showed an interesting profile of tocopherols, organic acids, unsaturated fatty acids, and phenolic compounds. The infused extract of the commercial leaf sample was more effective than other preparations against lipid peroxidation and oxidative haemolysis. In turn, higher antibacterial activity was achieved with hydroethanolic and infused extracts of the wild sample, which extracts also displayed superior antifungal activity. Overall, the commercially acquired M. oleifera leaves stand out with better quality profiles than the wild collected, which aroused interest in studying the processing methods used locally to process this functional food.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau",
volume = "43",
doi = "10.1016/j.fbio.2021.101300",
pages = "101300"
}

Fernandes, Â., Liberal, Â., Pinela, J., Finimundy, T. C., Bancessi, A., Ćirić, A., Soković, M., Catarino, L., Ferreira, I. C.F.R.,& Barros, L.. (2021). Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau. in Food Bioscience
Elsevier Ltd., 43, 101300.
https://doi.org/10.1016/j.fbio.2021.101300

Fernandes Â, Liberal Â, Pinela J, Finimundy TC, Bancessi A, Ćirić A, Soković M, Catarino L, Ferreira IC, Barros L. Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau. in Food Bioscience. 2021;43:101300.
doi:10.1016/j.fbio.2021.101300 .

Fernandes, Ângela, Liberal, Ângela, Pinela, José, Finimundy, Tiane C., Bancessi, Aducabe, Ćirić, Ana, Soković, Marina, Catarino, Luís, Ferreira, Isabel C.F.R., Barros, Lillian, "Compositional features and biological activities of wild and commercial Moringa oleifera leaves from Guinea-Bissau" in Food Bioscience, 43 (2021):101300,
https://doi.org/10.1016/j.fbio.2021.101300 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Calhelha, Ricardo
AU  - Fernandes, Ângela
AU  - Ferreira, Isabel
AU  - Stojković, Dejan
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51392100096N
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4761
AB  - Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.
PB  - Belgrade: Serbian Chemical Socitey
T2  - Journal of the Serbian Chemical Society
T1  - Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute
IS  - 12
VL  - 86
DO  - 10.2298/JSC210729096N
SP  - 1291
EP  - 1303
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Marković, Dejan and Calhelha, Ricardo and Fernandes, Ângela and Ferreira, Isabel and Stojković, Dejan and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2021",
abstract = "Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.",
publisher = "Belgrade: Serbian Chemical Socitey",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute",
number = "12",
volume = "86",
doi = "10.2298/JSC210729096N",
pages = "1291-1303"
}

Nikolić, M., Marković, T., Marković, D., Calhelha, R., Fernandes, Â., Ferreira, I., Stojković, D., Ćirić, A., Glamočlija, J.,& Soković, M.. (2021). Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Socitey., 86(12), 1291-1303.
https://doi.org/10.2298/JSC210729096N

Nikolić M, Marković T, Marković D, Calhelha R, Fernandes Â, Ferreira I, Stojković D, Ćirić A, Glamočlija J, Soković M. Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society. 2021;86(12):1291-1303.
doi:10.2298/JSC210729096N .

Nikolić, Miloš, Marković, Tatjana, Marković, Dejan, Calhelha, Ricardo, Fernandes, Ângela, Ferreira, Isabel, Stojković, Dejan, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute" in Journal of the Serbian Chemical Society, 86, no. 12 (2021):1291-1303,
https://doi.org/10.2298/JSC210729096N . .

TY  - JOUR
AU  - Cruz, Luís R. O.
AU  - Fernandes, Ângela
AU  - Di Gioia, Francesco
AU  - Petropoulos, Spyridon A.
AU  - Polyzos, Nikolaos
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/11/1036
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3967
AB  - In the present study, three red-colored (Dark Opal, Basilico Rosso, and Red Basil) and one green-colored landrace (Mitikas) of basil (Ocimum basilicum L.) were grown under four nitrogen regimes, namely Control (no fertilizer added), 200 ppm, 400 ppm, and 600 ppm of nitrogen (N). Fresh yield varied depending on N input following a quadratic function in all four genotypes, and green basil performed better compared to the red cultivars. A significant interaction of genotype × N input was recorded for most of the chemical parameters measured. Tocopherols contents of leaves were consistently higher in plants that received 200 ppm of N and lower in those receiving 600 ppm of N, especially in Dark Opal and Red Basil cultivars. Polyunsaturated fatty acids (PUFA) were the major category of fatty acids and Red Basil had the lowest ratio of omega-6/omega 3 (0.29) and thus the best fatty acid profile. Polyphenols content was the highest in Red Basil and Dark Opal (25 mg/g of extract on average) and the lowest in Mitikas and decreased with increasing N input. Similarly, antioxidant activity was the highest in Dark Opal and Red Basil fertigated with 200 ppm of N, whereas all the leaf extracts tested had good antibacterial and antifungal activity. In conclusion, basil chemical and bioactive profile was significantly influenced by both genotype and N input. Red-colored basil, although less productive, had the best chemical profile, and moderate levels of N input may provide the best compromise between yield, nutritional value, and bioactivity for the species.
PB  - MDPI AG
T2  - Antioxidants
T1  - The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars
IS  - 11
VL  - 9
DO  - 10.3390/antiox9111036
SP  - 1036
ER  - 

@article{
author = "Cruz, Luís R. O. and Fernandes, Ângela and Di Gioia, Francesco and Petropoulos, Spyridon A. and Polyzos, Nikolaos and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "In the present study, three red-colored (Dark Opal, Basilico Rosso, and Red Basil) and one green-colored landrace (Mitikas) of basil (Ocimum basilicum L.) were grown under four nitrogen regimes, namely Control (no fertilizer added), 200 ppm, 400 ppm, and 600 ppm of nitrogen (N). Fresh yield varied depending on N input following a quadratic function in all four genotypes, and green basil performed better compared to the red cultivars. A significant interaction of genotype × N input was recorded for most of the chemical parameters measured. Tocopherols contents of leaves were consistently higher in plants that received 200 ppm of N and lower in those receiving 600 ppm of N, especially in Dark Opal and Red Basil cultivars. Polyunsaturated fatty acids (PUFA) were the major category of fatty acids and Red Basil had the lowest ratio of omega-6/omega 3 (0.29) and thus the best fatty acid profile. Polyphenols content was the highest in Red Basil and Dark Opal (25 mg/g of extract on average) and the lowest in Mitikas and decreased with increasing N input. Similarly, antioxidant activity was the highest in Dark Opal and Red Basil fertigated with 200 ppm of N, whereas all the leaf extracts tested had good antibacterial and antifungal activity. In conclusion, basil chemical and bioactive profile was significantly influenced by both genotype and N input. Red-colored basil, although less productive, had the best chemical profile, and moderate levels of N input may provide the best compromise between yield, nutritional value, and bioactivity for the species.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars",
number = "11",
volume = "9",
doi = "10.3390/antiox9111036",
pages = "1036"
}

Cruz, L. R. O., Fernandes, Â., Di Gioia, F., Petropoulos, S. A., Polyzos, N., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars. in Antioxidants
MDPI AG., 9(11), 1036.
https://doi.org/10.3390/antiox9111036

Cruz LRO, Fernandes Â, Di Gioia F, Petropoulos SA, Polyzos N, Dias MI, Pinela J, Kostić M, Soković M, Ferreira ICFR, Barros L. The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars. in Antioxidants. 2020;9(11):1036.
doi:10.3390/antiox9111036 .

Cruz, Luís R. O., Fernandes, Ângela, Di Gioia, Francesco, Petropoulos, Spyridon A., Polyzos, Nikolaos, Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars" in Antioxidants, 9, no. 11 (2020):1036,
https://doi.org/10.3390/antiox9111036 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Plexida, Sofia
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2071-1050/12/20/8511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3943
AB  - The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.
PB  - MDPI AG
T2  - Sustainability
T1  - The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production
IS  - 20
VL  - 12
DO  - 10.3390/su12208511
SP  - 8511
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Plexida, Sofia and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Petrović, Jovana and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.",
publisher = "MDPI AG",
journal = "Sustainability",
title = "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production",
number = "20",
volume = "12",
doi = "10.3390/su12208511",
pages = "8511"
}

Petropoulos, S. A., Fernandes, Â., Plexida, S., Pereira, C., Dias, M. I., Calhelha, R., Chrysargyris, A., Tzortzakis, N., Petrović, J., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability
MDPI AG., 12(20), 8511.
https://doi.org/10.3390/su12208511

Petropoulos SA, Fernandes Â, Plexida S, Pereira C, Dias MI, Calhelha R, Chrysargyris A, Tzortzakis N, Petrović J, Soković M, Ferreira ICFR, Barros L. The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability. 2020;12(20):8511.
doi:10.3390/su12208511 .

Petropoulos, Spyridon A., Fernandes, Ângela, Plexida, Sofia, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo, Chrysargyris, Antonios, Tzortzakis, Nikolaos, Petrović, Jovana, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production" in Sustainability, 12, no. 20 (2020):8511,
https://doi.org/10.3390/su12208511 . .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Pinela, José
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/18/4336
UR  - https://radar.ibiss.bg.ac.rs/123456789/3905
AB  - This study explored the biological properties of three wild growing Russula species (R. integra, R. rosea, R. nigricans) from Serbia. Compositional features and antioxidant, antibacterial, antibiofilm, and cytotoxic activities were analyzed. The studied mushroom species were identified as being rich sources of carbohydrates and of low caloric value. Mannitol was the most abundant free sugar and quinic and malic acids the major organic acids detected. The four tocopherol isoforms were found, and polyunsaturated fatty acids were the predominant fat constituents. Regarding phenolic compounds, P-hydroxybenzoic and cinnamic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation and oxidative hemolysis; the highest activity was attributed to the R. nigricans ethanolic extract. This is the first report on the antibacterial and antibiofilm potential of the studied species, with the most promising activity observed towards Streptococcus spp. (0.20–0.78 mg/mL as the minimal inhibitory concentration, MIC). The most promising cytotoxic effect was caused by the R. integra methanolic extract on non-small cell lung cancer cells (NCI-H460). Therefore, due to the observed in vitro bioactive properties, the studied mushrooms arise as a source of functional ingredients with potential to be used in novel nutraceutical and drug formulations, which can be used in the treatment of various diseases and health conditions.
PB  - NLM (Medline)
T2  - Molecules
T1  - Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity
IS  - 18
VL  - 25
DO  - 10.3390/molecules25184336
SP  - 4336
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Pinela, José and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Ferreira, Isabel C. F. R. and Soković, Marina and Ćirić, Ana",
year = "2020",
abstract = "This study explored the biological properties of three wild growing Russula species (R. integra, R. rosea, R. nigricans) from Serbia. Compositional features and antioxidant, antibacterial, antibiofilm, and cytotoxic activities were analyzed. The studied mushroom species were identified as being rich sources of carbohydrates and of low caloric value. Mannitol was the most abundant free sugar and quinic and malic acids the major organic acids detected. The four tocopherol isoforms were found, and polyunsaturated fatty acids were the predominant fat constituents. Regarding phenolic compounds, P-hydroxybenzoic and cinnamic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation and oxidative hemolysis; the highest activity was attributed to the R. nigricans ethanolic extract. This is the first report on the antibacterial and antibiofilm potential of the studied species, with the most promising activity observed towards Streptococcus spp. (0.20–0.78 mg/mL as the minimal inhibitory concentration, MIC). The most promising cytotoxic effect was caused by the R. integra methanolic extract on non-small cell lung cancer cells (NCI-H460). Therefore, due to the observed in vitro bioactive properties, the studied mushrooms arise as a source of functional ingredients with potential to be used in novel nutraceutical and drug formulations, which can be used in the treatment of various diseases and health conditions.",
publisher = "NLM (Medline)",
journal = "Molecules",
title = "Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity",
number = "18",
volume = "25",
doi = "10.3390/molecules25184336",
pages = "4336"
}

Kostić, M., Ivanov, M., Fernandes, Â., Pinela, J., Calhelha, R. C., Glamočlija, J., Barros, L., Ferreira, I. C. F. R., Soković, M.,& Ćirić, A.. (2020). Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity. in Molecules
NLM (Medline)., 25(18), 4336.
https://doi.org/10.3390/molecules25184336

Kostić M, Ivanov M, Fernandes Â, Pinela J, Calhelha RC, Glamočlija J, Barros L, Ferreira ICFR, Soković M, Ćirić A. Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity. in Molecules. 2020;25(18):4336.
doi:10.3390/molecules25184336 .

Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Pinela, José, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Ferreira, Isabel C. F. R., Soković, Marina, Ćirić, Ana, "Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity" in Molecules, 25, no. 18 (2020):4336,
https://doi.org/10.3390/molecules25184336 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3175
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32664565
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3829
AB  - The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143175
SP  - 3175
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C F R and Barros, Lillian",
year = "2020",
abstract = "The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143175",
pages = "3175"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3175.
https://doi.org/10.3390/molecules25143175

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland). 2020;25(14):3175.
doi:10.3390/molecules25143175 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C F R, Barros, Lillian, "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3175,
https://doi.org/10.3390/molecules25143175 . .

TY  - JOUR
AU  - Finimundy, Tiane C.
AU  - Karkanis, Anestis
AU  - Fernandes, Ângela
AU  - Petropoulos, Spyridon A.
AU  - Calhelha, Ricardo
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Rosa, Eduardo
AU  - Barros, Lillian
AU  - Ferreira, Isabel C F R
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32470801
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3694
AB  - In this study, the chemical characterization and bioactive properties of S. minor cultivated under different fertilization rates (control, half rate and full rate) were evaluated. Twenty-two phenolic compounds were identified, including five phenolic acids, seven flavonoids and ten tannins. Hydrolysable tannins were prevalent, namely Sanguiin H-10, especially in leaves without fertilization (control). Roots of full-rate fertilizer (660 Kg/ha) presented the highest flavonoid content, mainly catechin and its isomers, whereas half-rate fertilizer (330 Kg/ha), presented the highest content of total phenolic compounds, due to the higher amount of ellagitannins (lambertianin C: 84 ± 1 mg/g of dry extract). Antimicrobial activities were also promising, especially against Salmonella typhimurium (MBC = 0.44 mg/mL). Moreover, root samples revealed activity against all tested cell lines regardless of fertilization rate, whereas leaves were effective only against HeLa cell line. In conclusion, S. minor could be a source of natural bioactive compounds, while fertilization could increase phenolic compounds content.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.
VL  - 327
DO  - 10.1016/j.foodchem.2020.127043
SP  - 127043
ER  - 

@article{
author = "Finimundy, Tiane C. and Karkanis, Anestis and Fernandes, Ângela and Petropoulos, Spyridon A. and Calhelha, Ricardo and Petrović, Jovana and Soković, Marina and Rosa, Eduardo and Barros, Lillian and Ferreira, Isabel C F R",
year = "2020",
abstract = "In this study, the chemical characterization and bioactive properties of S. minor cultivated under different fertilization rates (control, half rate and full rate) were evaluated. Twenty-two phenolic compounds were identified, including five phenolic acids, seven flavonoids and ten tannins. Hydrolysable tannins were prevalent, namely Sanguiin H-10, especially in leaves without fertilization (control). Roots of full-rate fertilizer (660 Kg/ha) presented the highest flavonoid content, mainly catechin and its isomers, whereas half-rate fertilizer (330 Kg/ha), presented the highest content of total phenolic compounds, due to the higher amount of ellagitannins (lambertianin C: 84 ± 1 mg/g of dry extract). Antimicrobial activities were also promising, especially against Salmonella typhimurium (MBC = 0.44 mg/mL). Moreover, root samples revealed activity against all tested cell lines regardless of fertilization rate, whereas leaves were effective only against HeLa cell line. In conclusion, S. minor could be a source of natural bioactive compounds, while fertilization could increase phenolic compounds content.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.",
volume = "327",
doi = "10.1016/j.foodchem.2020.127043",
pages = "127043"
}

Finimundy, T. C., Karkanis, A., Fernandes, Â., Petropoulos, S. A., Calhelha, R., Petrović, J., Soković, M., Rosa, E., Barros, L.,& Ferreira, I. C. F. R.. (2020). Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.. in Food Chemistry
Elsevier Ltd., 327, 127043.
https://doi.org/10.1016/j.foodchem.2020.127043

Finimundy TC, Karkanis A, Fernandes Â, Petropoulos SA, Calhelha R, Petrović J, Soković M, Rosa E, Barros L, Ferreira ICFR. Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.. in Food Chemistry. 2020;327:127043.
doi:10.1016/j.foodchem.2020.127043 .

Finimundy, Tiane C., Karkanis, Anestis, Fernandes, Ângela, Petropoulos, Spyridon A., Calhelha, Ricardo, Petrović, Jovana, Soković, Marina, Rosa, Eduardo, Barros, Lillian, Ferreira, Isabel C F R, "Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes." in Food Chemistry, 327 (2020):127043,
https://doi.org/10.1016/j.foodchem.2020.127043 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Ines
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/9/2204
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3675
AB  - The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.
PB  - MDPI AG
T2  - Molecules
T1  - Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions
IS  - 9
VL  - 25
DO  - 10.3390/molecules25092204
SP  - 2204
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Ines and Pereira, Carla and Calhelha, Ricardo C. and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.",
publisher = "MDPI AG",
journal = "Molecules",
title = "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions",
number = "9",
volume = "25",
doi = "10.3390/molecules25092204",
pages = "2204"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Chrysargyris, A., Tzortzakis, N., Ivanov, M., Soković, M., Barros, L.,& Ferreira, I. C. F. R.. (2020). Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules
MDPI AG., 25(9), 2204.
https://doi.org/10.3390/molecules25092204

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Chrysargyris A, Tzortzakis N, Ivanov M, Soković M, Barros L, Ferreira ICFR. Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules. 2020;25(9):2204.
doi:10.3390/molecules25092204 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Ines, Pereira, Carla, Calhelha, Ricardo C., Chrysargyris, Antonios, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C. F. R., "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions" in Molecules, 25, no. 9 (2020):2204,
https://doi.org/10.3390/molecules25092204 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Giannoulis, Kyriakos D.
AU  - Dias, Maria Inês
AU  - Fernandes, Ângela
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996920303069
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3674
AB  - Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.
PB  - Elsevier Ltd
T2  - Food Research International
T1  - Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis
VL  - 134
DO  - 10.1016/j.foodres.2020.109281
SP  - 109281
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Giannoulis, Kyriakos D. and Dias, Maria Inês and Fernandes, Ângela and Pinela, José and Kostić, Marina and Soković, Marina and Barros, Lillian and Santos-Buelga, Celestino and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.",
publisher = "Elsevier Ltd",
journal = "Food Research International",
title = "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis",
volume = "134",
doi = "10.1016/j.foodres.2020.109281",
pages = "109281"
}

Mandim, F., Petropoulos, S. A., Giannoulis, K. D., Dias, M. I., Fernandes, Â., Pinela, J., Kostić, M., Soković, M., Barros, L., Santos-Buelga, C.,& Ferreira, I. C.F.R.. (2020). Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International
Elsevier Ltd., 134, 109281.
https://doi.org/10.1016/j.foodres.2020.109281

Mandim F, Petropoulos SA, Giannoulis KD, Dias MI, Fernandes Â, Pinela J, Kostić M, Soković M, Barros L, Santos-Buelga C, Ferreira IC. Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International. 2020;134:109281.
doi:10.1016/j.foodres.2020.109281 .

Mandim, Filipa, Petropoulos, Spyridon A., Giannoulis, Kyriakos D., Dias, Maria Inês, Fernandes, Ângela, Pinela, José, Kostić, Marina, Soković, Marina, Barros, Lillian, Santos-Buelga, Celestino, Ferreira, Isabel C.F.R., "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis" in Food Research International, 134 (2020):109281,
https://doi.org/10.1016/j.foodres.2020.109281 . .

TY  - JOUR
AU  - Sampaio, Shirley L.
AU  - Fernandes, Ângela
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3671
AB  - Quinoa is a very interesting food due to its nutritional and chemical composition, as well as its bioactive properties, such as antioxidant and antimicrobial activity. Chenopodium quinoa Willd. is an ancient food crop that has provided nutrition to Andean populations for thousands of years. BRS Piabiru is a quinoa genotype developed and adapted to tropical climate by the Brazilian Agricultural Research Corporation. In this work, BRS Piabiru quinoa was evaluated concerning its nutritional, physicochemical and phenolic composition and also its bioactive properties. This variety showed high carbohydrate and protein contents and a low-fat level, composed of 86% of unsaturated fatty acids, 60% of which are polyunsaturated fatty acids. Four organic acids were detected, with quinic acid as the most abundant, while α- and γ-tocopherols were the vitamin E isoforms found. Quercetin and kaempferol glycosides were the main phenolic compounds in the quinoa extract, which also revealed relevant antioxidant and antimicrobial activities, with no toxic effect. These results support the potential of BRS Piabiru quinoa as a nutritious food crop and a source of bioactive compounds.
PB  - Royal Society of Chemistry (RSC)
T2  - Food & Function
T1  - Nutritional value, physicochemical characterization and bioactive properties of the Brazilian quinoa BRS Piabiru
IS  - 4
VL  - 11
DO  - 10.1039/d0fo00055h
SP  - 2969
EP  - 2977
ER  - 

@article{
author = "Sampaio, Shirley L. and Fernandes, Ângela and Pereira, Carla and Calhelha, Ricardo C. and Soković, Marina and Santos-Buelga, Celestino and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "Quinoa is a very interesting food due to its nutritional and chemical composition, as well as its bioactive properties, such as antioxidant and antimicrobial activity. Chenopodium quinoa Willd. is an ancient food crop that has provided nutrition to Andean populations for thousands of years. BRS Piabiru is a quinoa genotype developed and adapted to tropical climate by the Brazilian Agricultural Research Corporation. In this work, BRS Piabiru quinoa was evaluated concerning its nutritional, physicochemical and phenolic composition and also its bioactive properties. This variety showed high carbohydrate and protein contents and a low-fat level, composed of 86% of unsaturated fatty acids, 60% of which are polyunsaturated fatty acids. Four organic acids were detected, with quinic acid as the most abundant, while α- and γ-tocopherols were the vitamin E isoforms found. Quercetin and kaempferol glycosides were the main phenolic compounds in the quinoa extract, which also revealed relevant antioxidant and antimicrobial activities, with no toxic effect. These results support the potential of BRS Piabiru quinoa as a nutritious food crop and a source of bioactive compounds.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Food & Function",
title = "Nutritional value, physicochemical characterization and bioactive properties of the Brazilian quinoa BRS Piabiru",
number = "4",
volume = "11",
doi = "10.1039/d0fo00055h",
pages = "2969-2977"
}

Sampaio, S. L., Fernandes, Â., Pereira, C., Calhelha, R. C., Soković, M., Santos-Buelga, C., Barros, L.,& Ferreira, I. C. F. R.. (2020). Nutritional value, physicochemical characterization and bioactive properties of the Brazilian quinoa BRS Piabiru. in Food & Function
Royal Society of Chemistry (RSC)., 11(4), 2969-2977.
https://doi.org/10.1039/d0fo00055h

Sampaio SL, Fernandes Â, Pereira C, Calhelha RC, Soković M, Santos-Buelga C, Barros L, Ferreira ICFR. Nutritional value, physicochemical characterization and bioactive properties of the Brazilian quinoa BRS Piabiru. in Food & Function. 2020;11(4):2969-2977.
doi:10.1039/d0fo00055h .

Sampaio, Shirley L., Fernandes, Ângela, Pereira, Carla, Calhelha, Ricardo C., Soković, Marina, Santos-Buelga, Celestino, Barros, Lillian, Ferreira, Isabel C. F. R., "Nutritional value, physicochemical characterization and bioactive properties of the Brazilian quinoa BRS Piabiru" in Food & Function, 11, no. 4 (2020):2969-2977,
https://doi.org/10.1039/d0fo00055h . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Ines
AU  - Pereira, Carla
AU  - Calhelha, Ricardo
AU  - Di Gioia, Francesco
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/4/314
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3664
AB  - Centaurea raphanina subsp. mixta (DC.) Runemark is a wild edible species endemic to Greece. This study evaluated the chemical composition and bioactive properties of wild and cultivated C. raphanina subsp. mixta plants. Wild plants had higher nutritional value than cultivated ones, whereas cultivated plants contained more tocopherols. Glucose and sucrose were higher in cultivated plants and trehalose in wild ones. Oxalic and total organic acids were detected in higher amounts in cultivated samples. The main fatty acids were α-linolenic, linoleic and palmitic acid, while wild plants were richer in polyunsaturated fatty acids. Two pinocembrin derivatives were the main phenolic compounds being detected in higher amounts in wild plants. Regarding the antioxidant activity, wild and cultivated plants were more effective in the oxidative haemolysis (OxHLIA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Moreover, both extracts showed moderate cytotoxicity in non-tumor cell lines (PLP2), while cultivated plants were more effective against cervical carcinoma (HeLa), breast carcinoma (MCF-7) and non-small lung cancer (NCI-H460) cell lines. Finally, wild plants showed higher antimicrobial activity than cultivated plants against specific pathogens. In conclusion, the cultivation of C. raphanina subsp. mixta showed promising results in terms of tocopherols content and antiproliferative effects, however further research is needed to decrease oxalic acid content.
PB  - MDPI AG
T2  - Antioxidants
T1  - Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds
IS  - 4
VL  - 9
DO  - 10.3390/antiox9040314
SP  - 314
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Ines and Pereira, Carla and Calhelha, Ricardo and Di Gioia, Francesco and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "Centaurea raphanina subsp. mixta (DC.) Runemark is a wild edible species endemic to Greece. This study evaluated the chemical composition and bioactive properties of wild and cultivated C. raphanina subsp. mixta plants. Wild plants had higher nutritional value than cultivated ones, whereas cultivated plants contained more tocopherols. Glucose and sucrose were higher in cultivated plants and trehalose in wild ones. Oxalic and total organic acids were detected in higher amounts in cultivated samples. The main fatty acids were α-linolenic, linoleic and palmitic acid, while wild plants were richer in polyunsaturated fatty acids. Two pinocembrin derivatives were the main phenolic compounds being detected in higher amounts in wild plants. Regarding the antioxidant activity, wild and cultivated plants were more effective in the oxidative haemolysis (OxHLIA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Moreover, both extracts showed moderate cytotoxicity in non-tumor cell lines (PLP2), while cultivated plants were more effective against cervical carcinoma (HeLa), breast carcinoma (MCF-7) and non-small lung cancer (NCI-H460) cell lines. Finally, wild plants showed higher antimicrobial activity than cultivated plants against specific pathogens. In conclusion, the cultivation of C. raphanina subsp. mixta showed promising results in terms of tocopherols content and antiproliferative effects, however further research is needed to decrease oxalic acid content.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds",
number = "4",
volume = "9",
doi = "10.3390/antiox9040314",
pages = "314"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R., Di Gioia, F., Tzortzakis, N., Ivanov, M., Soković, M., Barros, L.,& Ferreira, I. C. F. R.. (2020). Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds. in Antioxidants
MDPI AG., 9(4), 314.
https://doi.org/10.3390/antiox9040314

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha R, Di Gioia F, Tzortzakis N, Ivanov M, Soković M, Barros L, Ferreira ICFR. Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds. in Antioxidants. 2020;9(4):314.
doi:10.3390/antiox9040314 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Ines, Pereira, Carla, Calhelha, Ricardo, Di Gioia, Francesco, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C. F. R., "Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds" in Antioxidants, 9, no. 4 (2020):314,
https://doi.org/10.3390/antiox9040314 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Arampatzis, Dimitrios A.
AU  - Tsiropoulos, Nikolaos G.
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - internal-pdf://Petropoulos et al. - 2020 - Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.) A new insight to plant-based sou.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3602
AB  - In the present study, nutritional value, chemical composition and bioactive properties of purslane seeds, seed oils and seedcakes were examined. Data were analyzed by a one-way ANOVA, while means were compared with Tukey's HSD test. For seed oil extraction mechanical and ultrasound assisted methods were tested. Cold extraction methods (CE1 and CE2) resulted in higher oil yield (increased by 33.7% and 38.1%, respectively) comparing to hot extraction (HE) method. Seeds contained the highest amount of fats and energy (15.03 ± 0.06 g/100 g dry basis (db) and 459 ± 1 kcal/100 g db, respectively), while seedcakes from CE2 had the highest content in proteins and ash (31.20 ± 0.03 and 4.27 ± 0.06 g/100 g db, respectively). Seeds and seedcakes contained a balanced content of linoleic and α-linolenic acids (33.80–34.74% and 32.83–34.64%, respectively). HE and CE1 oils had slightly higher amounts of α-linolenic (39.67% and 39.57%, respectively) than linoleic acid (35.44% and 35.13%, respectively), whereas CE2 oils contained twice as much linoleic as α-linolenic acid (49.77% and 24.18%, respectively). In conclusion, the tested materials are good sources of omega-6 and omega-3 fatty acids and proteins, while extraction method affected oil yield and fatty acids composition of seed oils.
T2  - LWT
T1  - Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids
VL  - 123
DO  - 10.1016/j.lwt.2020.109099
SP  - 109099
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Arampatzis, Dimitrios A. and Tsiropoulos, Nikolaos G. and Petrović, Jovana and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "In the present study, nutritional value, chemical composition and bioactive properties of purslane seeds, seed oils and seedcakes were examined. Data were analyzed by a one-way ANOVA, while means were compared with Tukey's HSD test. For seed oil extraction mechanical and ultrasound assisted methods were tested. Cold extraction methods (CE1 and CE2) resulted in higher oil yield (increased by 33.7% and 38.1%, respectively) comparing to hot extraction (HE) method. Seeds contained the highest amount of fats and energy (15.03 ± 0.06 g/100 g dry basis (db) and 459 ± 1 kcal/100 g db, respectively), while seedcakes from CE2 had the highest content in proteins and ash (31.20 ± 0.03 and 4.27 ± 0.06 g/100 g db, respectively). Seeds and seedcakes contained a balanced content of linoleic and α-linolenic acids (33.80–34.74% and 32.83–34.64%, respectively). HE and CE1 oils had slightly higher amounts of α-linolenic (39.67% and 39.57%, respectively) than linoleic acid (35.44% and 35.13%, respectively), whereas CE2 oils contained twice as much linoleic as α-linolenic acid (49.77% and 24.18%, respectively). In conclusion, the tested materials are good sources of omega-6 and omega-3 fatty acids and proteins, while extraction method affected oil yield and fatty acids composition of seed oils.",
journal = "LWT",
title = "Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids",
volume = "123",
doi = "10.1016/j.lwt.2020.109099",
pages = "109099"
}

Petropoulos, S. A., Fernandes, Â., Arampatzis, D. A., Tsiropoulos, N. G., Petrović, J., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2020). Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids. in LWT, 123, 109099.
https://doi.org/10.1016/j.lwt.2020.109099

Petropoulos SA, Fernandes Â, Arampatzis DA, Tsiropoulos NG, Petrović J, Soković M, Barros L, Ferreira IC. Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids. in LWT. 2020;123:109099.
doi:10.1016/j.lwt.2020.109099 .

Petropoulos, Spyridon A., Fernandes, Ângela, Arampatzis, Dimitrios A., Tsiropoulos, Nikolaos G., Petrović, Jovana, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids" in LWT, 123 (2020):109099,
https://doi.org/10.1016/j.lwt.2020.109099 . .

TY  - JOUR
AU  - Cruz, Luís R. O.
AU  - Polyzos, Nikolaos
AU  - Fernandes, Ângela
AU  - Petropoulos, Spyridon A.
AU  - Gioia, Francesco Di
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2073-4395/10/11/1824
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4093
AB  - The present study investigated the effect of salinity (Control: 1.8 dS/m, S1: 3.0 dS/m and S2: 4.5 dS/m) on the chemical composition and bioactive properties of three basil cultivars (Red Basil, Dark Opal Red and Basilico Rosso). Crop performance was not affected by increasing salinity in DoR and BaR. Fat, ash and carbohydrates content increased by salinity in DoR, whereas energetic value was negatively affected. Free sugars (total and individual compounds) increased under saline conditions (S2) in BaR, whereas reducing trends were observed for the main organic acids and tocopherols in all the cultivars. The major fatty acids were α-linolenic, linoleic and palmitic acids with no consistent salinity effects, while the richest polyphenols were sagerinic acid and eriodictyol-O-malonylhexoside. Finally, basil extracts showed moderate antioxidant and strong antifungal activity. In conclusion, salinity showed a genotype dependent effect on the chemical profile and bioactivities of the tested cultivars.
PB  - MDPI AG
T2  - Agronomy
T1  - Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars
IS  - 11
VL  - 10
DO  - 10.3390/agronomy10111824
SP  - 1824
ER  - 

@article{
author = "Cruz, Luís R. O. and Polyzos, Nikolaos and Fernandes, Ângela and Petropoulos, Spyridon A. and Gioia, Francesco Di and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "The present study investigated the effect of salinity (Control: 1.8 dS/m, S1: 3.0 dS/m and S2: 4.5 dS/m) on the chemical composition and bioactive properties of three basil cultivars (Red Basil, Dark Opal Red and Basilico Rosso). Crop performance was not affected by increasing salinity in DoR and BaR. Fat, ash and carbohydrates content increased by salinity in DoR, whereas energetic value was negatively affected. Free sugars (total and individual compounds) increased under saline conditions (S2) in BaR, whereas reducing trends were observed for the main organic acids and tocopherols in all the cultivars. The major fatty acids were α-linolenic, linoleic and palmitic acids with no consistent salinity effects, while the richest polyphenols were sagerinic acid and eriodictyol-O-malonylhexoside. Finally, basil extracts showed moderate antioxidant and strong antifungal activity. In conclusion, salinity showed a genotype dependent effect on the chemical profile and bioactivities of the tested cultivars.",
publisher = "MDPI AG",
journal = "Agronomy",
title = "Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars",
number = "11",
volume = "10",
doi = "10.3390/agronomy10111824",
pages = "1824"
}

Cruz, L. R. O., Polyzos, N., Fernandes, Â., Petropoulos, S. A., Gioia, F. D., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars. in Agronomy
MDPI AG., 10(11), 1824.
https://doi.org/10.3390/agronomy10111824

Cruz LRO, Polyzos N, Fernandes Â, Petropoulos SA, Gioia FD, Dias MI, Pinela J, Kostić M, Soković M, Ferreira ICFR, Barros L. Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars. in Agronomy. 2020;10(11):1824.
doi:10.3390/agronomy10111824 .

Cruz, Luís R. O., Polyzos, Nikolaos, Fernandes, Ângela, Petropoulos, Spyridon A., Gioia, Francesco Di, Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars" in Agronomy, 10, no. 11 (2020):1824,
https://doi.org/10.3390/agronomy10111824 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A
AU  - Taofiq, Oludemi
AU  - Fernandes, Ângela
AU  - Tzortzakis, Nikos
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel CFR
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.9881
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3445
AB  - BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.
T2  - Journal of the Science of Food and Agriculture
T1  - Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.
DO  - 10.1002/jsfa.9881
ER  - 

@article{
author = "Petropoulos, Spyridon A and Taofiq, Oludemi and Fernandes, Ângela and Tzortzakis, Nikos and Ćirić, Ana and Soković, Marina and Barros, Lillian and Ferreira, Isabel CFR",
year = "2019",
abstract = "BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.",
journal = "Journal of the Science of Food and Agriculture",
title = "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.",
doi = "10.1002/jsfa.9881"
}

Petropoulos, S. A., Taofiq, O., Fernandes, Â., Tzortzakis, N., Ćirić, A., Soković, M., Barros, L.,& Ferreira, I. C.. (2019). Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture.
https://doi.org/10.1002/jsfa.9881

Petropoulos SA, Taofiq O, Fernandes Â, Tzortzakis N, Ćirić A, Soković M, Barros L, Ferreira IC. Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture. 2019;.
doi:10.1002/jsfa.9881 .

Petropoulos, Spyridon A, Taofiq, Oludemi, Fernandes, Ângela, Tzortzakis, Nikos, Ćirić, Ana, Soković, Marina, Barros, Lillian, Ferreira, Isabel CFR, "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect." in Journal of the Science of Food and Agriculture (2019),
https://doi.org/10.1002/jsfa.9881 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Kruljević, Ivana
AU  - Stojković, Dejan
AU  - Fernandes, Ângela
AU  - Barros, Lillian
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2019
UR  - http://xlink.rsc.org/?DOI=C9FO01900F
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3576
AB  - This study was oriented towards the investigation of the biological properties of three wild growing and edible Macrolepiota species (M. mastoidea, M. rhacodes and Macrolepiota procera) from Serbia. The results revealed that the mushrooms have a low caloric value; free sugars such as mannitol and trehalose were identified; oxalic and malic acids were predominant organic acids, while p-hydroxybenzoic and p-coumaric acids were identified as the main phenolic compounds. Also, they were a rich source of polyunsaturated fatty acids, which dominated over monounsaturated and saturated fatty acids. Three isoforms of tocopherols were identified and quantified: α-, β-, and δ-tocopherol. Regarding biological properties, all three species exhibited antioxidant potential, antimicrobial potential and cytotoxic activity within the different tumour cell lines tested. This study indicates that these species are indeed functional foods, due to the fact that they are edible, consumable and hold different pharmacological activities.
PB  - The Royal Society of Chemistry
T2  - Food & Function
T1  - Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.
IS  - 12
VL  - 10
DO  - 10.1039/c9fo01900f
SP  - 7678
EP  - 7686
ER  - 

@article{
author = "Ćirić, Ana and Kruljević, Ivana and Stojković, Dejan and Fernandes, Ângela and Barros, Lillian and Calhelha, Ricardo C. and Ferreira, Isabel C. F. R. and Soković, Marina and Glamočlija, Jasmina",
year = "2019",
abstract = "This study was oriented towards the investigation of the biological properties of three wild growing and edible Macrolepiota species (M. mastoidea, M. rhacodes and Macrolepiota procera) from Serbia. The results revealed that the mushrooms have a low caloric value; free sugars such as mannitol and trehalose were identified; oxalic and malic acids were predominant organic acids, while p-hydroxybenzoic and p-coumaric acids were identified as the main phenolic compounds. Also, they were a rich source of polyunsaturated fatty acids, which dominated over monounsaturated and saturated fatty acids. Three isoforms of tocopherols were identified and quantified: α-, β-, and δ-tocopherol. Regarding biological properties, all three species exhibited antioxidant potential, antimicrobial potential and cytotoxic activity within the different tumour cell lines tested. This study indicates that these species are indeed functional foods, due to the fact that they are edible, consumable and hold different pharmacological activities.",
publisher = "The Royal Society of Chemistry",
journal = "Food & Function",
title = "Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.",
number = "12",
volume = "10",
doi = "10.1039/c9fo01900f",
pages = "7678-7686"
}

Ćirić, A., Kruljević, I., Stojković, D., Fernandes, Â., Barros, L., Calhelha, R. C., Ferreira, I. C. F. R., Soković, M.,& Glamočlija, J.. (2019). Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.. in Food & Function
The Royal Society of Chemistry., 10(12), 7678-7686.
https://doi.org/10.1039/c9fo01900f

Ćirić A, Kruljević I, Stojković D, Fernandes Â, Barros L, Calhelha RC, Ferreira ICFR, Soković M, Glamočlija J. Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities.. in Food & Function. 2019;10(12):7678-7686.
doi:10.1039/c9fo01900f .

Ćirić, Ana, Kruljević, Ivana, Stojković, Dejan, Fernandes, Ângela, Barros, Lillian, Calhelha, Ricardo C., Ferreira, Isabel C. F. R., Soković, Marina, Glamočlija, Jasmina, "Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: functional foods with diverse biological activities." in Food & Function, 10, no. 12 (2019):7678-7686,
https://doi.org/10.1039/c9fo01900f . .