TY  - JOUR
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6553
AB  - Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.
PB  - Wiley
T2  - CNS Neuroscience and Therapeutics
T1  - The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease
IS  - 3
VL  - 30
DO  - 10.1111/cns.14188
SP  - e14188
ER  - 

@article{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2024",
abstract = "Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.",
publisher = "Wiley",
journal = "CNS Neuroscience and Therapeutics",
title = "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease",
number = "3",
volume = "30",
doi = "10.1111/cns.14188",
pages = "e14188"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2024). The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics
Wiley., 30(3), e14188.
https://doi.org/10.1111/cns.14188

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics. 2024;30(3):e14188.
doi:10.1111/cns.14188 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease" in CNS Neuroscience and Therapeutics, 30, no. 3 (2024):e14188,
https://doi.org/10.1111/cns.14188 . .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Živanović, Ana
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Major, Tamara
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6548
AB  - Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease
IS  - 18
VL  - 24
DO  - 10.3390/ijms241814092
SP  - 14092
ER  - 

@article{
author = "Jovanović Macura, Irena and Živanović, Ana and Perović, Milka and Ćirić, Jelena and Major, Tamara and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease",
number = "18",
volume = "24",
doi = "10.3390/ijms241814092",
pages = "14092"
}

Jovanović Macura, I., Živanović, A., Perović, M., Ćirić, J., Major, T., Kanazir, S.,& Ivković, S.. (2023). The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences
Basel: MDPI., 24(18), 14092.
https://doi.org/10.3390/ijms241814092

Jovanović Macura I, Živanović A, Perović M, Ćirić J, Major T, Kanazir S, Ivković S. The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences. 2023;24(18):14092.
doi:10.3390/ijms241814092 .

Jovanović Macura, Irena, Živanović, Ana, Perović, Milka, Ćirić, Jelena, Major, Tamara, Kanazir, Selma, Ivković, Sanja, "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease" in International Journal of Molecular Sciences, 24, no. 18 (2023):14092,
https://doi.org/10.3390/ijms241814092 . .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5847
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5847
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5847"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5846
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5846
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5846"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5842
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5842
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5842"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):110,
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5843
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5843
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5843"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5843

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Ivković, Sanja
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599456
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5172
AB  - Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101297
SP  - 1297
ER  - 

@article{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Ivković, Sanja and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101297",
pages = "1297"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Ivković, S., Kanazir, S.,& Perović, M.. (2022). Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences
Basel: MDPI., 12(10), 1297.
https://doi.org/10.3390/brainsci12101297

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Ivković S, Kanazir S, Perović M. Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences. 2022;12(10):1297.
doi:10.3390/brainsci12101297 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Ivković, Sanja, Kanazir, Selma, Perović, Milka, "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging" in Brain Sciences, 12, no. 10 (2022):1297,
https://doi.org/10.3390/brainsci12101297 . .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pešić, Vesna
AU  - Hofman, Katarina
AU  - Perović, Milka
AU  - Ćirić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5616
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti
T1  - Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести
SP  - 382
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5616
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pešić, Vesna and Hofman, Katarina and Perović, Milka and Ćirić, Jelena",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti, Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести",
pages = "382",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5616"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pešić, V., Hofman, K., Perović, M.,& Ćirić, J.. (2022). Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616

Milovanović N, Jovanović Macura I, Tešić V, Pešić V, Hofman K, Perović M, Ćirić J. Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pešić, Vesna, Hofman, Katarina, Perović, Milka, Ćirić, Jelena, "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):382,
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Hofman, Katarina
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5613
AB  - Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа.
AB  - Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti
T1  - Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести
SP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5613
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Hofman, Katarina and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа., Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti, Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести",
pages = "343",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5613"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Hofman, K., Kanazir, S.,& Perović, M.. (2022). Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Hofman K, Kanazir S, Perović M. Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Hofman, Katarina, Kanazir, Selma, Perović, Milka, "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):343,
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5615
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti
T1  - Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести
SP  - 317
SP  - M64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5615
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti, Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести",
pages = "317-M64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5615"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):317,
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5187
AB  - Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice
DO  - 10.1002/2211-5463.13440
SP  - 132
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice",
doi = "10.1002/2211-5463.13440",
pages = "132"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Kanazir, S.,& Perović, M.. (2022). Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 132.
https://doi.org/10.1002/2211-5463.13440

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Kanazir S, Perović M. Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:132.
doi:10.1002/2211-5463.13440 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Kanazir, Selma, Perović, Milka, "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):132,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5188
AB  - The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease
DO  - 10.1002/2211-5463.13440
SP  - 130
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease",
doi = "10.1002/2211-5463.13440",
pages = "130"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 130.
https://doi.org/10.1002/2211-5463.13440

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:130.
doi:10.1002/2211-5463.13440 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):130,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - JOUR
AU  - Tešić, Vesna
AU  - Ćirić, Jelena
AU  - Jovanović Macura, Irena
AU  - Zogović, Nevena
AU  - Milanović, Desanka
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2021
UR  - https://www.mdpi.com/2072-6643/13/12/4526
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8703853
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4760
AB  - Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.
IS  - 12
VL  - 13
DO  - 10.3390/nu13124526
SP  - 4526
ER  - 

@article{
author = "Tešić, Vesna and Ćirić, Jelena and Jovanović Macura, Irena and Zogović, Nevena and Milanović, Desanka and Kanazir, Selma and Perović, Milka",
year = "2021",
abstract = "Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.",
number = "12",
volume = "13",
doi = "10.3390/nu13124526",
pages = "4526"
}

Tešić, V., Ćirić, J., Jovanović Macura, I., Zogović, N., Milanović, D., Kanazir, S.,& Perović, M.. (2021). Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients
Basel: MDPI., 13(12), 4526.
https://doi.org/10.3390/nu13124526

Tešić V, Ćirić J, Jovanović Macura I, Zogović N, Milanović D, Kanazir S, Perović M. Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients. 2021;13(12):4526.
doi:10.3390/nu13124526 .

Tešić, Vesna, Ćirić, Jelena, Jovanović Macura, Irena, Zogović, Nevena, Milanović, Desanka, Kanazir, Selma, Perović, Milka, "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction." in Nutrients, 13, no. 12 (2021):4526,
https://doi.org/10.3390/nu13124526 . .

TY  - JOUR
AU  - Rajković, Nemanja
AU  - Ćirić, Jelena
AU  - Milošević, Nebojša
AU  - Šaponjić, Jasna
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0010482519303518?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3487
AB  - To reveal the best choice of algorithm for parvalbumin-immunostained images of the hippocampal gyrus dentatus in two distinct rat models of Parkinson's disease (PD), particularly in terms of extracting the crucial information from the image, we tested whether the impact of experimentally induced dopaminergic (hemiparkinsonism) vs. cholinergic (PD cholinopathy) innervation impairment on the parvalbumin stained GABA interneurons could be detected using two separate algorithms, the fractal box-count and the gray-level co-occurrence matrix analysis (GLCM) algorithms. For the texture and fractal analysis of the hippocampal gyrus dentatus images, we used.tif images from three experimental groups of adult male Wistar rats: control rats, rats with Parkinson disease (PD) cholinergic neuropathology (with a PPT lesion), and hemiparkinsonian rats (with a SNpc lesion). For the suprapyramidal layer of the gyrus dentatus ASM and Entropy differentiated the images of the SNpc lesion versus the images of the control and the PPT lesion subjects, with significantly higher ASM and lower Entropy, indicating the homogenization of the images and their lower gray-level complexity. The infrapyramidal images of the SNpc group were differentiated versus the images from the control and PPT groups in terms of all the GLCM parameters: they showed lower mean Entropy and Contrast and higher ASM, Correlation and IDM. These results strongly suggest a rise in the uniformity, homogeneity and orderliness in the gray-levels of images from the SNpc group. Our results indicate that GLCM analysis is a more sensitive tool than fractal analysis for the detection of increased dendritic arborization in histological images.
T2  - Computers in Biology and Medicine
T1  - Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.
VL  - 115
DO  - 10.1016/j.compbiomed.2019.103482
SP  - 103482
ER  - 

@article{
author = "Rajković, Nemanja and Ćirić, Jelena and Milošević, Nebojša and Šaponjić, Jasna",
year = "2019",
abstract = "To reveal the best choice of algorithm for parvalbumin-immunostained images of the hippocampal gyrus dentatus in two distinct rat models of Parkinson's disease (PD), particularly in terms of extracting the crucial information from the image, we tested whether the impact of experimentally induced dopaminergic (hemiparkinsonism) vs. cholinergic (PD cholinopathy) innervation impairment on the parvalbumin stained GABA interneurons could be detected using two separate algorithms, the fractal box-count and the gray-level co-occurrence matrix analysis (GLCM) algorithms. For the texture and fractal analysis of the hippocampal gyrus dentatus images, we used.tif images from three experimental groups of adult male Wistar rats: control rats, rats with Parkinson disease (PD) cholinergic neuropathology (with a PPT lesion), and hemiparkinsonian rats (with a SNpc lesion). For the suprapyramidal layer of the gyrus dentatus ASM and Entropy differentiated the images of the SNpc lesion versus the images of the control and the PPT lesion subjects, with significantly higher ASM and lower Entropy, indicating the homogenization of the images and their lower gray-level complexity. The infrapyramidal images of the SNpc group were differentiated versus the images from the control and PPT groups in terms of all the GLCM parameters: they showed lower mean Entropy and Contrast and higher ASM, Correlation and IDM. These results strongly suggest a rise in the uniformity, homogeneity and orderliness in the gray-levels of images from the SNpc group. Our results indicate that GLCM analysis is a more sensitive tool than fractal analysis for the detection of increased dendritic arborization in histological images.",
journal = "Computers in Biology and Medicine",
title = "Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.",
volume = "115",
doi = "10.1016/j.compbiomed.2019.103482",
pages = "103482"
}

Rajković, N., Ćirić, J., Milošević, N.,& Šaponjić, J.. (2019). Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.. in Computers in Biology and Medicine, 115, 103482.
https://doi.org/10.1016/j.compbiomed.2019.103482

Rajković N, Ćirić J, Milošević N, Šaponjić J. Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease.. in Computers in Biology and Medicine. 2019;115:103482.
doi:10.1016/j.compbiomed.2019.103482 .

Rajković, Nemanja, Ćirić, Jelena, Milošević, Nebojša, Šaponjić, Jasna, "Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson's disease." in Computers in Biology and Medicine, 115 (2019):103482,
https://doi.org/10.1016/j.compbiomed.2019.103482 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Kapor, Slobodan
AU  - Perović, Milka
AU  - Šaponjić, Jasna
PY  - 2019
UR  - https://www.frontiersin.org/article/10.3389/fnins.2019.00148/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6401659
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3294
AB  - Our previous studies in the rat model of Parkinson's disease (PD) cholinopathy demonstrated the sleep-related alterations in electroencephalographic (EEG) oscillations at the cortical and hippocampal levels, cortical drives, and sleep spindles (SSs) as the earliest functional biomarkers preceding hypokinesia. Our aim in this study was to follow the impact of a unilateral substantia nigra pars compacta (SNpc) lesion in rat on the cortical and hippocampal sleep architectures and their EEG microstructures, as well as the cortico-hippocampal synchronizations of EEG oscillations, and the SS and high voltage sleep spindle (HVS) dynamics during NREM and REM sleep. We performed unilateral SNpc lesions using two different concentrations/volumes of 6-hydroxydopamine (6-OHDA; 12 μg/1 μl or 12 μg/2 μl). Whereas the unilateral dopaminergic neuronal loss >50% throughout the overall SNpc rostro-caudal dimension prolonged the Wake state, with no change in the NREM or REM duration, there was a long-lasting theta amplitude augmentation across all sleep states in the motor cortex (MCx), but also in the CA1 hippocampus (Hipp) during both Wake and REM sleep. We demonstrate that SS are the hallmarks of NREM sleep, but that they also occur during REM sleep in the MCx and Hipp of the control rats. Whereas SS are always longer in REM vs. NREM sleep in both structures, they are consistently slower in the Hipp. The dopaminergic neuronal loss increased the density of SS in both structures and shortened them in the MCx during NREM sleep, without changing the intrinsic frequency. Conversely, HVS are the hallmarks of REM sleep in the control rats, slower in the Hipp vs. MCx, and the dopaminergic neuronal loss increased their density in the MCx, but shortened them more consistently in the Hipp during REM sleep. In addition, there was an altered synchronization of the EEG oscillations between the MCx and Hipp in different sleep states, particularly the theta and sigma coherences during REM sleep. We provide novel evidence for the importance of the SNpc dopaminergic innervation in sleep regulation, theta rhythm generation, and SS/HVS dynamics control. We suggest the importance of the underlying REM sleep regulatory substrate to HVS generation and duration and to the cortico-hippocampal synchronizations of EEG oscillations in hemiparkinsonian rats.
T2  - Frontiers in Neuroscience
T1  - Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.
VL  - 13
DO  - 10.3389/fnins.2019.00148
SP  - 148
ER  - 

@article{
author = "Ćirić, Jelena and Kapor, Slobodan and Perović, Milka and Šaponjić, Jasna",
year = "2019",
abstract = "Our previous studies in the rat model of Parkinson's disease (PD) cholinopathy demonstrated the sleep-related alterations in electroencephalographic (EEG) oscillations at the cortical and hippocampal levels, cortical drives, and sleep spindles (SSs) as the earliest functional biomarkers preceding hypokinesia. Our aim in this study was to follow the impact of a unilateral substantia nigra pars compacta (SNpc) lesion in rat on the cortical and hippocampal sleep architectures and their EEG microstructures, as well as the cortico-hippocampal synchronizations of EEG oscillations, and the SS and high voltage sleep spindle (HVS) dynamics during NREM and REM sleep. We performed unilateral SNpc lesions using two different concentrations/volumes of 6-hydroxydopamine (6-OHDA; 12 μg/1 μl or 12 μg/2 μl). Whereas the unilateral dopaminergic neuronal loss >50% throughout the overall SNpc rostro-caudal dimension prolonged the Wake state, with no change in the NREM or REM duration, there was a long-lasting theta amplitude augmentation across all sleep states in the motor cortex (MCx), but also in the CA1 hippocampus (Hipp) during both Wake and REM sleep. We demonstrate that SS are the hallmarks of NREM sleep, but that they also occur during REM sleep in the MCx and Hipp of the control rats. Whereas SS are always longer in REM vs. NREM sleep in both structures, they are consistently slower in the Hipp. The dopaminergic neuronal loss increased the density of SS in both structures and shortened them in the MCx during NREM sleep, without changing the intrinsic frequency. Conversely, HVS are the hallmarks of REM sleep in the control rats, slower in the Hipp vs. MCx, and the dopaminergic neuronal loss increased their density in the MCx, but shortened them more consistently in the Hipp during REM sleep. In addition, there was an altered synchronization of the EEG oscillations between the MCx and Hipp in different sleep states, particularly the theta and sigma coherences during REM sleep. We provide novel evidence for the importance of the SNpc dopaminergic innervation in sleep regulation, theta rhythm generation, and SS/HVS dynamics control. We suggest the importance of the underlying REM sleep regulatory substrate to HVS generation and duration and to the cortico-hippocampal synchronizations of EEG oscillations in hemiparkinsonian rats.",
journal = "Frontiers in Neuroscience",
title = "Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.",
volume = "13",
doi = "10.3389/fnins.2019.00148",
pages = "148"
}

Ćirić, J., Kapor, S., Perović, M.,& Šaponjić, J.. (2019). Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.. in Frontiers in Neuroscience, 13, 148.
https://doi.org/10.3389/fnins.2019.00148

Ćirić J, Kapor S, Perović M, Šaponjić J. Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat.. in Frontiers in Neuroscience. 2019;13:148.
doi:10.3389/fnins.2019.00148 .

Ćirić, Jelena, Kapor, Slobodan, Perović, Milka, Šaponjić, Jasna, "Alterations of Sleep and Sleep Oscillations in the Hemiparkinsonian Rat." in Frontiers in Neuroscience, 13 (2019):148,
https://doi.org/10.3389/fnins.2019.00148 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Kapor, Slobodan
AU  - Perović, Milka
AU  - Petrović, Jelena
AU  - Pešić, Vesna
AU  - Kanazir, Selma
AU  - Šaponjić, Jasna
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0166432817312391
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29170000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3361
AB  - In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.
T2  - Behavioural Brain Research
T1  - Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat
VL  - 339
DO  - 10.1016/j.bbr.2017.11.021
SP  - 79
EP  - 92
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Kapor, Slobodan and Perović, Milka and Petrović, Jelena and Pešić, Vesna and Kanazir, Selma and Šaponjić, Jasna",
year = "2018",
abstract = "In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.",
journal = "Behavioural Brain Research",
title = "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat",
volume = "339",
doi = "10.1016/j.bbr.2017.11.021",
pages = "79-92"
}

Ćirić, J., Lazić, K., Kapor, S., Perović, M., Petrović, J., Pešić, V., Kanazir, S.,& Šaponjić, J.. (2018). Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research, 339, 79-92.
https://doi.org/10.1016/j.bbr.2017.11.021

Ćirić J, Lazić K, Kapor S, Perović M, Petrović J, Pešić V, Kanazir S, Šaponjić J. Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research. 2018;339:79-92.
doi:10.1016/j.bbr.2017.11.021 .

Ćirić, Jelena, Lazić, Katarina, Kapor, Slobodan, Perović, Milka, Petrović, Jelena, Pešić, Vesna, Kanazir, Selma, Šaponjić, Jasna, "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat" in Behavioural Brain Research, 339 (2018):79-92,
https://doi.org/10.1016/j.bbr.2017.11.021 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Kapor, Slobodan
AU  - Perović, Milka
AU  - Petrović, Jelena
AU  - Pešić, Vesna
AU  - Kanazir, Selma
AU  - Šaponjić, Jasna
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0166432817312391
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29170000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2932
AB  - In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.
T2  - Behavioural Brain Research
T1  - Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat
VL  - 339
DO  - 10.1016/j.bbr.2017.11.021
SP  - 79
EP  - 92
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Kapor, Slobodan and Perović, Milka and Petrović, Jelena and Pešić, Vesna and Kanazir, Selma and Šaponjić, Jasna",
year = "2018",
abstract = "In order to find out the possible earliest biomarkers of Parkinson's disease (PD) cholinopathy, we followed the impact of bilateral pedunculopontine tegmental nucleus (PPT) lesion in rat on: the cortical and hippocampal sleep/wake states architectures, all sleep states related EEG microstructures, sleep spindles, the basal and stimulated locomotor activity. Sleep and basal locomotor activity in adult Wistar rats were followed during their inactive circadian phase, and throughout the same aging period. The bilateral PPT lesions were done by 0.1M ibotenic acid (IBO) during the surgical procedure for implantation of the electroencephalographic (EEG) and electromyographic (EMG) electrodes for chronic sleep recording. The cholinergic neuronal loss was identified by NADPH - diaphorase histochemistry. After all sleep and behavioral recording sessions, the locomotor activity was stimulated by d-amphetamine (d-AMPH) and the neuronal activity of striatum was followed by c-Fos immunolabeling. Impaired cholinergic innervation from the PPT was expressed earlier as sleep disorder then as movement disorder, and it was the earliest and long-lasting at hippocampal and thalamo-cortical level, and followed by a delayed "hypokinesia". This severe impact of a tonically impaired PPT cholinergic innervation was evidenced as the cholinergic interneuronal loss of the caudate putamen and as a suppressed c-Fos expression after stimulation by d-AMPH. In order how they occurred, the hippocampal non rapid eye movement (NREM) sleep disorder, altered high voltage sleep spindle (HVS) dynamics during rapid eye movement (REM) sleep in the hippocampus and motor cortex, and "hypokinesia" may serve as the biomarkers of PD cholinopathy onset and progression.",
journal = "Behavioural Brain Research",
title = "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat",
volume = "339",
doi = "10.1016/j.bbr.2017.11.021",
pages = "79-92"
}

Ćirić, J., Lazić, K., Kapor, S., Perović, M., Petrović, J., Pešić, V., Kanazir, S.,& Šaponjić, J.. (2018). Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research, 339, 79-92.
https://doi.org/10.1016/j.bbr.2017.11.021

Ćirić J, Lazić K, Kapor S, Perović M, Petrović J, Pešić V, Kanazir S, Šaponjić J. Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat. in Behavioural Brain Research. 2018;339:79-92.
doi:10.1016/j.bbr.2017.11.021 .

Ćirić, Jelena, Lazić, Katarina, Kapor, Slobodan, Perović, Milka, Petrović, Jelena, Pešić, Vesna, Kanazir, Selma, Šaponjić, Jasna, "Sleep disorder and altered locomotor activity as biomarkers of the Parkinson's disease cholinopathy in rat" in Behavioural Brain Research, 339 (2018):79-92,
https://doi.org/10.1016/j.bbr.2017.11.021 . .

TY  - JOUR
AU  - Lazić, Katarina
AU  - Ćirić, Jelena
AU  - Šaponjić, Jasna
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jsr.12758
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3140
AB  - On the basis of our previous studies and the important role of the thalamo-cortical network in states of unconsciousness, such as anaesthesia and sleep, and in sleep spindles generation, we investigated sleep spindles (SS) and high-voltage sleep spindle (HVS) dynamics during non-rapid eye movement (NREM) and rapid eye movement (REM) sleep following different types of general anaesthesia in both physiological controls and in a rat model of Parkinson's disease (PD) cholinopathy, to follow the impact of anaesthesia on post-anaesthesia sleep at the thalamo-cortical level through an altered sleep spindle dynamics. We recorded 6 hr of spontaneous sleep in all rats, both before and 48 hr after ketamine/diazepam or pentobarbital anaesthesia, and we used 1 hr of NREM or REM sleep from each to validate visually the automatically detected SS or HVS for their extraction and analysis. In the controls, SS occurred mainly during NREM, whereas HVS occurred only during REM sleep. Ketamine/diazepam anaesthesia promoted HVS, prolonged SS during NREM, induced HVS of increased frequency during REM, and increased SS/HVS densities during REM versus NREM sleep. Pentobarbital anaesthesia decreased the frequency of SS during NREM and the HVS density during REM sleep. Although the pedunculopontine tegmental nucleus lesion prolonged SS only during NREM sleep, in these rats, ketamine/diazepam anaesthesia suppressed HVS during both sleep states, whereas pentobarbital anaesthesia promoted HVS during REM sleep. The different impacts of two anaesthetic regimens on the thalamo-cortical regulatory network are expressed through their distinct sleep spindle generation and dynamics that are dependent on the NREM and REM state regulatory neuronal substrate.
T2  - Journal of Sleep Research
T1  - Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy
DO  - 10.1111/jsr.12758
ER  - 

@article{
author = "Lazić, Katarina and Ćirić, Jelena and Šaponjić, Jasna",
year = "2018",
abstract = "On the basis of our previous studies and the important role of the thalamo-cortical network in states of unconsciousness, such as anaesthesia and sleep, and in sleep spindles generation, we investigated sleep spindles (SS) and high-voltage sleep spindle (HVS) dynamics during non-rapid eye movement (NREM) and rapid eye movement (REM) sleep following different types of general anaesthesia in both physiological controls and in a rat model of Parkinson's disease (PD) cholinopathy, to follow the impact of anaesthesia on post-anaesthesia sleep at the thalamo-cortical level through an altered sleep spindle dynamics. We recorded 6 hr of spontaneous sleep in all rats, both before and 48 hr after ketamine/diazepam or pentobarbital anaesthesia, and we used 1 hr of NREM or REM sleep from each to validate visually the automatically detected SS or HVS for their extraction and analysis. In the controls, SS occurred mainly during NREM, whereas HVS occurred only during REM sleep. Ketamine/diazepam anaesthesia promoted HVS, prolonged SS during NREM, induced HVS of increased frequency during REM, and increased SS/HVS densities during REM versus NREM sleep. Pentobarbital anaesthesia decreased the frequency of SS during NREM and the HVS density during REM sleep. Although the pedunculopontine tegmental nucleus lesion prolonged SS only during NREM sleep, in these rats, ketamine/diazepam anaesthesia suppressed HVS during both sleep states, whereas pentobarbital anaesthesia promoted HVS during REM sleep. The different impacts of two anaesthetic regimens on the thalamo-cortical regulatory network are expressed through their distinct sleep spindle generation and dynamics that are dependent on the NREM and REM state regulatory neuronal substrate.",
journal = "Journal of Sleep Research",
title = "Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy",
doi = "10.1111/jsr.12758"
}

Lazić, K., Ćirić, J.,& Šaponjić, J.. (2018). Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy. in Journal of Sleep Research.
https://doi.org/10.1111/jsr.12758

Lazić K, Ćirić J, Šaponjić J. Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy. in Journal of Sleep Research. 2018;.
doi:10.1111/jsr.12758 .

Lazić, Katarina, Ćirić, Jelena, Šaponjić, Jasna, "Sleep spindle dynamics during NREM and REM sleep following distinct general anaesthesia in control rats and in a rat model of Parkinson’s disease cholinopathy" in Journal of Sleep Research (2018),
https://doi.org/10.1111/jsr.12758 . .

TY  - THES
AU  - Ćirić, Jelena
PY  - 2017
UR  - http://uvidok.rcub.bg.ac.rs/handle/123456789/1886
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2801
AB  - The aim of the present doctoral dissertation was to evaluate the impact
of aging during sleep in the rat models of Alzheimer’s and Parkinson’s disease
cholinergic neuropathology, and to determine the possible different and earlier
onset of age-related sleep disorder during healthy aging and aging with the
neurodegenerative diseases.
We used the bilateral nucleus basalis (NB) and nucleus pedunculopontinus
tegmentalis (PPT) lesioned rats as the in vivo models of functionally distinct
cholinergic neuropathology, and we followed the impact of aging on sleep
architecture, the electroencephalographic (EEG) microstructure and motor
control across sleep/wake states.
We evidenced the topographically distinct impact of healthy aging on
sleep architecture and motor control within the sensorimotor (SMCx) vs. motor
cortex (MCx). Whereas healthy aging consistently altered only the SMCx sleep
architecture, it increased the delta and beta cortical drives from both cortices
during Wake, but only through the MCx drive during rapid eye movement
sleep (REM).
Our results have shown for the first time that the earliest signs of aging
during distinct cholinergic neuropathology were expressed through a different
and topographically specific EEG microstructure during REM. EEG delta
amplitude attenuation within the SMCx was the earliest sign of aging in the NB
lesion, whereas EEG sigma amplitude augmentation within the MCx was the
earliest sign of aging in the PPT lesion during REM. In addition, aging was
differently expressed through the SMCx drive alterations, but it was commonly
expressed through the MCx drive alterations during all sleep/wake states.
This doctoral dissertation provided for the first time an evidence of
distinct REM sleep disorders and sleep state related cortical drives as the signs
of aging onset during functionally distinct cholinergic neuropathologies.
AB  - Cilj ove doktorske disertacije bio je da ispita uticaj starenja na spavanje u
eksperimentalnim modelima holinergičke neuropatologije Alchajmerove i
Parkinsonove bolesti, i da pronađe najranije znake poremećaja spavanja u
fiziološkom starenju i u starenju sa neurodegenerativnim bolestima.
Uticaj starenja na arhitekturu spavanja, elektroencefalografsku (EEG)
mikrostrukturu i motornu kontrolu, u toku svake faze spavanja, je praćen u
eksperimentalnim modelima bilateralnih oštećenja jedara nucleus basalis (NB) i
nucleus pedunculopontinus tegmentalis (PPT) u pacova, kao eksperimentalnim in
vivo modelima funkcionalno različitih holinergičkih neuropatologija.
Fiziološko starenje dovodi do topografski različitih promena arhitekture
spavanja i motorne kontrole iz senzomotorne kore (SMCx) u odnosu na
motornu koru (MCx). Pored promena arhitekture spavanja koje su se javile
samo u SMCx, fiziološko starenje dovodi i do povećanja propagacije delta i beta
oscilacija iz obe kore za vreme budnosti, ali samo iz MCx za vreme REM faze
spavanja.
Najraniji znaci starenja u eksperimentalnim modelima funkcionalno
različitih holinergičkih neuropatologija, dokazani po prvi put, predstavljaju
topografski specifične razlike u EEG mikrostrukturi za vreme REM faze
spavanja. Smanjenje delta EEG relativne amplitude u SMCx predstavlja najraniji
znak starenja kod NB lediranih pacova, dok povećanje sigma EEG relativne
amplitude u MCx predstavlja najraniji znak starenja kod PPT lediranih pacova,
za vreme REM faze spavanja. Pored toga, starenjem izazvane promene su
različito izražene kroz mišićnu kontrolu iz SMCx, a istovremeno i istovetno
izražene iz MCx u toku svih faza spavanja.
Ova doktorska disertacija je po prvi put dokazala različite poremećaje
REM faze spavanja i motorne kontrole u toku spavanja, kao najranije znake
početka starenja u funkcionalno različitim holinergičkim neuropatologijama.
PB  - University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Rani elektrofiziološki znaci poremećaja spavanja i motorne kontrole u starenju pacova sa neurodegeneracijom holinergičkih neurona
T1  - Early electrophysiological markers of the sleep and motor control disorders during aging in rats with neurodegeneration of the cholinergic neurons
SP  - 1
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2801
ER  - 

@phdthesis{
author = "Ćirić, Jelena",
year = "2017",
abstract = "The aim of the present doctoral dissertation was to evaluate the impact
of aging during sleep in the rat models of Alzheimer’s and Parkinson’s disease
cholinergic neuropathology, and to determine the possible different and earlier
onset of age-related sleep disorder during healthy aging and aging with the
neurodegenerative diseases.
We used the bilateral nucleus basalis (NB) and nucleus pedunculopontinus
tegmentalis (PPT) lesioned rats as the in vivo models of functionally distinct
cholinergic neuropathology, and we followed the impact of aging on sleep
architecture, the electroencephalographic (EEG) microstructure and motor
control across sleep/wake states.
We evidenced the topographically distinct impact of healthy aging on
sleep architecture and motor control within the sensorimotor (SMCx) vs. motor
cortex (MCx). Whereas healthy aging consistently altered only the SMCx sleep
architecture, it increased the delta and beta cortical drives from both cortices
during Wake, but only through the MCx drive during rapid eye movement
sleep (REM).
Our results have shown for the first time that the earliest signs of aging
during distinct cholinergic neuropathology were expressed through a different
and topographically specific EEG microstructure during REM. EEG delta
amplitude attenuation within the SMCx was the earliest sign of aging in the NB
lesion, whereas EEG sigma amplitude augmentation within the MCx was the
earliest sign of aging in the PPT lesion during REM. In addition, aging was
differently expressed through the SMCx drive alterations, but it was commonly
expressed through the MCx drive alterations during all sleep/wake states.
This doctoral dissertation provided for the first time an evidence of
distinct REM sleep disorders and sleep state related cortical drives as the signs
of aging onset during functionally distinct cholinergic neuropathologies., Cilj ove doktorske disertacije bio je da ispita uticaj starenja na spavanje u
eksperimentalnim modelima holinergičke neuropatologije Alchajmerove i
Parkinsonove bolesti, i da pronađe najranije znake poremećaja spavanja u
fiziološkom starenju i u starenju sa neurodegenerativnim bolestima.
Uticaj starenja na arhitekturu spavanja, elektroencefalografsku (EEG)
mikrostrukturu i motornu kontrolu, u toku svake faze spavanja, je praćen u
eksperimentalnim modelima bilateralnih oštećenja jedara nucleus basalis (NB) i
nucleus pedunculopontinus tegmentalis (PPT) u pacova, kao eksperimentalnim in
vivo modelima funkcionalno različitih holinergičkih neuropatologija.
Fiziološko starenje dovodi do topografski različitih promena arhitekture
spavanja i motorne kontrole iz senzomotorne kore (SMCx) u odnosu na
motornu koru (MCx). Pored promena arhitekture spavanja koje su se javile
samo u SMCx, fiziološko starenje dovodi i do povećanja propagacije delta i beta
oscilacija iz obe kore za vreme budnosti, ali samo iz MCx za vreme REM faze
spavanja.
Najraniji znaci starenja u eksperimentalnim modelima funkcionalno
različitih holinergičkih neuropatologija, dokazani po prvi put, predstavljaju
topografski specifične razlike u EEG mikrostrukturi za vreme REM faze
spavanja. Smanjenje delta EEG relativne amplitude u SMCx predstavlja najraniji
znak starenja kod NB lediranih pacova, dok povećanje sigma EEG relativne
amplitude u MCx predstavlja najraniji znak starenja kod PPT lediranih pacova,
za vreme REM faze spavanja. Pored toga, starenjem izazvane promene su
različito izražene kroz mišićnu kontrolu iz SMCx, a istovremeno i istovetno
izražene iz MCx u toku svih faza spavanja.
Ova doktorska disertacija je po prvi put dokazala različite poremećaje
REM faze spavanja i motorne kontrole u toku spavanja, kao najranije znake
početka starenja u funkcionalno različitim holinergičkim neuropatologijama.",
publisher = "University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Rani elektrofiziološki znaci poremećaja spavanja i motorne kontrole u starenju pacova sa neurodegeneracijom holinergičkih neurona, Early electrophysiological markers of the sleep and motor control disorders during aging in rats with neurodegeneration of the cholinergic neurons",
pages = "1-147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2801"
}

Ćirić, J.. (2017). Rani elektrofiziološki znaci poremećaja spavanja i motorne kontrole u starenju pacova sa neurodegeneracijom holinergičkih neurona. in University of Belgrade, Faculty of Biology
University of Belgrade, Faculty of Biology., 1-147.
https://hdl.handle.net/21.15107/rcub_ibiss_2801

Ćirić J. Rani elektrofiziološki znaci poremećaja spavanja i motorne kontrole u starenju pacova sa neurodegeneracijom holinergičkih neurona. in University of Belgrade, Faculty of Biology. 2017;:1-147.
https://hdl.handle.net/21.15107/rcub_ibiss_2801 .

Ćirić, Jelena, "Rani elektrofiziološki znaci poremećaja spavanja i motorne kontrole u starenju pacova sa neurodegeneracijom holinergičkih neurona" in University of Belgrade, Faculty of Biology (2017):1-147,
https://hdl.handle.net/21.15107/rcub_ibiss_2801 .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Kalauzi, A
AU  - Šaponjić, Jasna
PY  - 2017
UR  - http://www.oatext.com/sleep-spindles-as-an-early-biomarker-of-rem-sleep-disorder-in-a-rat-model-of-parkinsons-disease-cholinopathy.php
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3296
AB  - Rhythmic oscillations of neuronal populations, generated by different mechanisms, are present at several levels of the central nervous system and serve many important physiological or reflect pathological functions. Understanding the role of brain oscillations as possible biomarkers of brain function and plasticity is still a challenge, and despite extensive research, their role is still not well established. We recently demonstrated that the hallmarks of earlier aging onset during impaired thalamo-cortical cholinergic innervation (in a rat model of Parkinson’s disease cholinopathy) were consistently expressed, from 3 and one half to 5 and one half months of age, through increased electroencephalographic (EEG) sigma activity amplitude during rapid eye movement (REM) sleep, as a unique aging induced REM sleep phenomenon. In addition, there was altered motor cortical drive during non-rapid-eye-movement (NREM) and REM sleep. In order to explain this new aging-induced REM sleep phenomenon, we analyzed possible differences between control REM sleep spindle activity and REM sleep spindle activity at the onset of REM sleep “enriched“ with sigma activity (at 4 and one half months of age), following bilateral pedunculopontine tegmental nucleus (PPT) cholinergic neuronal loss in the rat. We analzyed differences in spindle density, duration, and frequency. We demonstrated in young adult Wistar rats with the severely impaired PPT cholinergic innervation the alterations in sleep spindle dynamics and pattern during REM sleep in the motor cortex as the earliest biomarkers for the onset of their altered aging processes.
T2  - Translational Brain Rhythmicity
T1  - Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy
IS  - 1
VL  - 2
DO  - 10.15761/TBR.1000111
SP  - 1
EP  - 11
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Petrović, Jelena and Kalauzi, A and Šaponjić, Jasna",
year = "2017",
abstract = "Rhythmic oscillations of neuronal populations, generated by different mechanisms, are present at several levels of the central nervous system and serve many important physiological or reflect pathological functions. Understanding the role of brain oscillations as possible biomarkers of brain function and plasticity is still a challenge, and despite extensive research, their role is still not well established. We recently demonstrated that the hallmarks of earlier aging onset during impaired thalamo-cortical cholinergic innervation (in a rat model of Parkinson’s disease cholinopathy) were consistently expressed, from 3 and one half to 5 and one half months of age, through increased electroencephalographic (EEG) sigma activity amplitude during rapid eye movement (REM) sleep, as a unique aging induced REM sleep phenomenon. In addition, there was altered motor cortical drive during non-rapid-eye-movement (NREM) and REM sleep. In order to explain this new aging-induced REM sleep phenomenon, we analyzed possible differences between control REM sleep spindle activity and REM sleep spindle activity at the onset of REM sleep “enriched“ with sigma activity (at 4 and one half months of age), following bilateral pedunculopontine tegmental nucleus (PPT) cholinergic neuronal loss in the rat. We analzyed differences in spindle density, duration, and frequency. We demonstrated in young adult Wistar rats with the severely impaired PPT cholinergic innervation the alterations in sleep spindle dynamics and pattern during REM sleep in the motor cortex as the earliest biomarkers for the onset of their altered aging processes.",
journal = "Translational Brain Rhythmicity",
title = "Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy",
number = "1",
volume = "2",
doi = "10.15761/TBR.1000111",
pages = "1-11"
}

Ćirić, J., Lazić, K., Petrović, J., Kalauzi, A.,& Šaponjić, J.. (2017). Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy. in Translational Brain Rhythmicity, 2(1), 1-11.
https://doi.org/10.15761/TBR.1000111

Ćirić J, Lazić K, Petrović J, Kalauzi A, Šaponjić J. Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy. in Translational Brain Rhythmicity. 2017;2(1):1-11.
doi:10.15761/TBR.1000111 .

Ćirić, Jelena, Lazić, Katarina, Petrović, Jelena, Kalauzi, A, Šaponjić, Jasna, "Sleep spindles as an early biomarker of REM sleep disorder in a rat model of Parkinson’s disease cholinopathy" in Translational Brain Rhythmicity, 2, no. 1 (2017):1-11,
https://doi.org/10.15761/TBR.1000111 . .

TY  - JOUR
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Ćirić, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0031938416306308
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3297
AB  - Postoperative sleep disorders, particularly the REM sleep disorder, may have a significant deleterious impact on postoperative outcomes and may contribute to the genesis of certain delayed postoperative complications. We have followed the effect of distinct anesthesia regimens (ketamine/diazepam vs. pentobarbital) over 6days following the induction of a stable anesthetized state in adult male Wistar rats, chronically instrumented for sleep recording. In order to compare the effect of both anesthetics in the physiological controls vs. the rats with impaired pedunculopontine tegmental nucleus (PPT) cholinergic innervation, during the operative procedure for the implantation of EEG and EMG electrodes, the bilateral PPT lesion was conducted using ibotenic acid (IBO). We have followed in particular post-anesthesia REM sleep. Our results show the distinct EEG microstructure of the motor cortex during the different stable anesthetized states, and their distinct impact on post-anesthesia REM sleep. In contrast to pentobarbital anesthesia, the ketamine/diazepam anesthesia potentiated the long-lasting post-anesthesia REM statewith higher muscle tone (REM1) vs. REM state with atonia (REM2). Whereas both anesthesias prolonged the post-anesthesia REM sleep duration, the long-term prolongation of the REM1 state was demonstrated only after the ketamine/diazepam anesthesia, first due to the increased number of REM1 episodes, and then due to the prolonged REM1 episodes duration. On the other hand, whereas both anesthetic regimens abolished the prolonged post-anesthesia REM/REM1 sleep and the EEG microstructure disorder during REM sleep, only the pentobarbital abolished the increased NREM/REM/NREM transitions, caused by the PPT lesion. In addition, in the PPT lesioned rats, the ketamine/diazepam anesthesia decreased the Wake/NREM/Wake transitions while the pentobarbital anesthesia decreased the Wake/REM/Wake transitions. Our present study suggests pentobarbital anesthesia as being highly beneficial for post-anesthesia REM sleep in the physiological condition as well as during PPT cholinergic neuropathology.
T2  - Physiology & Behavior
T1  - REM sleep disorder following general anesthesia in rats.
VL  - 168
DO  - 10.1016/j.physbeh.2016.10.013
SP  - 41
EP  - 54
ER  - 

@article{
author = "Lazić, Katarina and Petrović, Jelena and Ćirić, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2017",
abstract = "Postoperative sleep disorders, particularly the REM sleep disorder, may have a significant deleterious impact on postoperative outcomes and may contribute to the genesis of certain delayed postoperative complications. We have followed the effect of distinct anesthesia regimens (ketamine/diazepam vs. pentobarbital) over 6days following the induction of a stable anesthetized state in adult male Wistar rats, chronically instrumented for sleep recording. In order to compare the effect of both anesthetics in the physiological controls vs. the rats with impaired pedunculopontine tegmental nucleus (PPT) cholinergic innervation, during the operative procedure for the implantation of EEG and EMG electrodes, the bilateral PPT lesion was conducted using ibotenic acid (IBO). We have followed in particular post-anesthesia REM sleep. Our results show the distinct EEG microstructure of the motor cortex during the different stable anesthetized states, and their distinct impact on post-anesthesia REM sleep. In contrast to pentobarbital anesthesia, the ketamine/diazepam anesthesia potentiated the long-lasting post-anesthesia REM statewith higher muscle tone (REM1) vs. REM state with atonia (REM2). Whereas both anesthesias prolonged the post-anesthesia REM sleep duration, the long-term prolongation of the REM1 state was demonstrated only after the ketamine/diazepam anesthesia, first due to the increased number of REM1 episodes, and then due to the prolonged REM1 episodes duration. On the other hand, whereas both anesthetic regimens abolished the prolonged post-anesthesia REM/REM1 sleep and the EEG microstructure disorder during REM sleep, only the pentobarbital abolished the increased NREM/REM/NREM transitions, caused by the PPT lesion. In addition, in the PPT lesioned rats, the ketamine/diazepam anesthesia decreased the Wake/NREM/Wake transitions while the pentobarbital anesthesia decreased the Wake/REM/Wake transitions. Our present study suggests pentobarbital anesthesia as being highly beneficial for post-anesthesia REM sleep in the physiological condition as well as during PPT cholinergic neuropathology.",
journal = "Physiology & Behavior",
title = "REM sleep disorder following general anesthesia in rats.",
volume = "168",
doi = "10.1016/j.physbeh.2016.10.013",
pages = "41-54"
}

Lazić, K., Petrović, J., Ćirić, J., Kalauzi, A.,& Šaponjić, J.. (2017). REM sleep disorder following general anesthesia in rats.. in Physiology & Behavior, 168, 41-54.
https://doi.org/10.1016/j.physbeh.2016.10.013

Lazić K, Petrović J, Ćirić J, Kalauzi A, Šaponjić J. REM sleep disorder following general anesthesia in rats.. in Physiology & Behavior. 2017;168:41-54.
doi:10.1016/j.physbeh.2016.10.013 .

Lazić, Katarina, Petrović, Jelena, Ćirić, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "REM sleep disorder following general anesthesia in rats." in Physiology & Behavior, 168 (2017):41-54,
https://doi.org/10.1016/j.physbeh.2016.10.013 . .

TY  - CHAP
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
PY  - 2016
UR  - http://dx.doi.org/10.5772/62949
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2474
UR  - http://www.intechopen.com/books/challenges-in-parkinson-s-disease/disorders-of-sleep-and-motor-control-during-the-impaired-cholinergic-innervation-in-rat-relevance-to
AB  - The medical profession has been generally very slow to acknowledge the importance of sleep medicine and sleep research. Disorders of sleep are related to anxiety, many mental and neurodegenerative diseases, cardiovascular and respiratory disorders, and obesity. Our knowledge of the neural substrates of sleep/wake states and sleep-related behavior disorders regulation in health and the diseases, over more than 50 years of sleep research, is based on the experiments in animal models, pharmacotherapy, and the neuropathological studies in humans. But, we still need further work in fundamental multidisciplinary and clinical research between sleep and neurodegenerative disease investigators to understand normal and abnormal sleep, and to provide new insights into preventive or disease-altering approaches for therapy. Our aim is to give an overview of our recent results related to the importance of thalamo-cortical cholinergic brain system in the disorders of sleep and motor control during sleep, with particular relevance to Parkinson’s disease.
PB  - Rijeka: InTech
T2  - Challenges in Parkinson’s Disease
T1  - Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease
DO  - 10.5772/62949
SP  - 136
EP  - 153
ER  - 

@inbook{
author = "Šaponjić, Jasna and Petrović, Jelena and Ćirić, Jelena and Lazić, Katarina",
year = "2016",
abstract = "The medical profession has been generally very slow to acknowledge the importance of sleep medicine and sleep research. Disorders of sleep are related to anxiety, many mental and neurodegenerative diseases, cardiovascular and respiratory disorders, and obesity. Our knowledge of the neural substrates of sleep/wake states and sleep-related behavior disorders regulation in health and the diseases, over more than 50 years of sleep research, is based on the experiments in animal models, pharmacotherapy, and the neuropathological studies in humans. But, we still need further work in fundamental multidisciplinary and clinical research between sleep and neurodegenerative disease investigators to understand normal and abnormal sleep, and to provide new insights into preventive or disease-altering approaches for therapy. Our aim is to give an overview of our recent results related to the importance of thalamo-cortical cholinergic brain system in the disorders of sleep and motor control during sleep, with particular relevance to Parkinson’s disease.",
publisher = "Rijeka: InTech",
journal = "Challenges in Parkinson’s Disease",
booktitle = "Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease",
doi = "10.5772/62949",
pages = "136-153"
}

Šaponjić, J., Petrović, J., Ćirić, J.,& Lazić, K.. (2016). Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease. in Challenges in Parkinson’s Disease
Rijeka: InTech., 136-153.
https://doi.org/10.5772/62949

Šaponjić J, Petrović J, Ćirić J, Lazić K. Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease. in Challenges in Parkinson’s Disease. 2016;:136-153.
doi:10.5772/62949 .

Šaponjić, Jasna, Petrović, Jelena, Ćirić, Jelena, Lazić, Katarina, "Disorders of Sleep and Motor Control During the Impaired Cholinergic Innervation in Rat – Relevance to Parkinson’s Disease" in Challenges in Parkinson’s Disease (2016):136-153,
https://doi.org/10.5772/62949 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1909
UR  - http://www.sciencedirect.com/science/article/pii/S0166432815303454
AB  - We studied the impact of aging during sleep in the rat models of
   Alzheimer's (AD) and Parkinson's (PD) disease cholinergic neuropathology
   to determine the possible different and earlier onset of age-related
   sleep disorder during the neurodegenerative diseases vs. healthy aging.
   We used the bilateral nucleus basalis (NB) and pedunculopontine
   tegmental nucleus (PPT) lesioned rats as the in vivo models of
   functionally distinct cholinergic neuropathology, and we followed the
   impact of aging on sleep architecture, the electroencephalographic (EEG)
   microstructure and motor control across sleep/wake states.
   Our results have shown for the first time that the earliest signs of
   aging during distinct cholinergic neuropathology were expressed through
   a different and topographically specific EEG microstructure during rapid
   eye movement sleep (REM). EEG delta amplitude attenuation within the
   sensorimotor cortex (SMCx) during REM was the earliest sign of aging in
   the NB lesion. EEG sigma amplitude augmentation within the motor cortex
   (MCx) during REM was the earliest sign of aging in the PPT lesion. In
   addition, aging was differently expressed through the SMCx drive
   alterations, but it was commonly expressed through the MCx drive
   alterations during all sleep/wake states.
   Our study provided evidence of distinct REM sleep disorders and sleep
   state related cortical drives as the signs of aging onset during
   functionally distinct cholinergic neuropathologies (NB lesion vs. PPT
   lesion). (C) 2015 Elsevier B.V. All rights reserved.
T2  - Behavioural Brain Research
T1  - Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology
VL  - 301
DO  - 10.1016/j.bbr.2015.12.046
SP  - 273
EP  - 286
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Petrović, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2016",
abstract = "We studied the impact of aging during sleep in the rat models of
   Alzheimer's (AD) and Parkinson's (PD) disease cholinergic neuropathology
   to determine the possible different and earlier onset of age-related
   sleep disorder during the neurodegenerative diseases vs. healthy aging.
   We used the bilateral nucleus basalis (NB) and pedunculopontine
   tegmental nucleus (PPT) lesioned rats as the in vivo models of
   functionally distinct cholinergic neuropathology, and we followed the
   impact of aging on sleep architecture, the electroencephalographic (EEG)
   microstructure and motor control across sleep/wake states.
   Our results have shown for the first time that the earliest signs of
   aging during distinct cholinergic neuropathology were expressed through
   a different and topographically specific EEG microstructure during rapid
   eye movement sleep (REM). EEG delta amplitude attenuation within the
   sensorimotor cortex (SMCx) during REM was the earliest sign of aging in
   the NB lesion. EEG sigma amplitude augmentation within the motor cortex
   (MCx) during REM was the earliest sign of aging in the PPT lesion. In
   addition, aging was differently expressed through the SMCx drive
   alterations, but it was commonly expressed through the MCx drive
   alterations during all sleep/wake states.
   Our study provided evidence of distinct REM sleep disorders and sleep
   state related cortical drives as the signs of aging onset during
   functionally distinct cholinergic neuropathologies (NB lesion vs. PPT
   lesion). (C) 2015 Elsevier B.V. All rights reserved.",
journal = "Behavioural Brain Research",
title = "Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology",
volume = "301",
doi = "10.1016/j.bbr.2015.12.046",
pages = "273-286"
}

Ćirić, J., Lazić, K., Petrović, J., Kalauzi, A.,& Šaponjić, J.. (2016). Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology. in Behavioural Brain Research, 301, 273-286.
https://doi.org/10.1016/j.bbr.2015.12.046

Ćirić J, Lazić K, Petrović J, Kalauzi A, Šaponjić J. Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology. in Behavioural Brain Research. 2016;301:273-286.
doi:10.1016/j.bbr.2015.12.046 .

Ćirić, Jelena, Lazić, Katarina, Petrović, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology" in Behavioural Brain Research, 301 (2016):273-286,
https://doi.org/10.1016/j.bbr.2015.12.046 . .

TY  - JOUR
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
AU  - Kalauzi, Aleksandar
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Radulovacki, Miodrag
AU  - Carley, David W
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1027
AB  - We examined the effects of unilateral and bilateral nucleus basalis (NB) lesion in rat on sleep/wake states, and sleep/wake state-related electroencephalographic (EEG) frequency relative amplitude distributions. We aimed this study to identify the possible EEG markers for the onset and progression of cortical cholinergic neurodegeneration in rats. NB lesion was performed by ibotenic acid (IBO) microinfusion, and identified by NADPH-diaphorase histochemistry. Sleep/wake states related EEG relative amplitude analysis was done using the Probability Density Estimate (PDE) routine supplied with MATLAB 6.5. Bilateral NB lesion transiently altered gross sleep/wake states topography 14 days following lesion. While control rats exhibited equivalent durations of Wake, NREM and REM, as determined by sensorimotor versus motor cortex EEG, bilateral NB lesion decreased Wake duration in both cortices, with NREM duration increased within sensorimotor cortex, and REM duration increased within motor cortex. Also, Wake, NREM and REM theta relative amplitude was lower in motor versus sensorimotor cortex in all groups of animals. In sensorimotor cortex bilateral NB lesion increased only REM theta relative amplitude from 1421 days following lesion, and returned to control value 28 days following lesion. In motor cortex both Wake and REM theta relative amplitude transiently increased 14 days following unilateral and bilateral NB lesion, and returned to control values 21 days after lesions. We demonstrated at functional level, for the first time, the topographically specific impact of NB cholinergic cortical afferent system dysregulation on sleep/wake states, REM and Wake EEG theta relative amplitude.
T2  - Sleep and Biological Rhythms
T1  - Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder
IS  - 2
VL  - 11
SP  - 243
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1027
ER  - 

@article{
author = "Šaponjić, Jasna and Petrović, Jelena and Kalauzi, Aleksandar and Ćirić, Jelena and Lazić, Katarina and Radulovacki, Miodrag and Carley, David W",
year = "2013",
abstract = "We examined the effects of unilateral and bilateral nucleus basalis (NB) lesion in rat on sleep/wake states, and sleep/wake state-related electroencephalographic (EEG) frequency relative amplitude distributions. We aimed this study to identify the possible EEG markers for the onset and progression of cortical cholinergic neurodegeneration in rats. NB lesion was performed by ibotenic acid (IBO) microinfusion, and identified by NADPH-diaphorase histochemistry. Sleep/wake states related EEG relative amplitude analysis was done using the Probability Density Estimate (PDE) routine supplied with MATLAB 6.5. Bilateral NB lesion transiently altered gross sleep/wake states topography 14 days following lesion. While control rats exhibited equivalent durations of Wake, NREM and REM, as determined by sensorimotor versus motor cortex EEG, bilateral NB lesion decreased Wake duration in both cortices, with NREM duration increased within sensorimotor cortex, and REM duration increased within motor cortex. Also, Wake, NREM and REM theta relative amplitude was lower in motor versus sensorimotor cortex in all groups of animals. In sensorimotor cortex bilateral NB lesion increased only REM theta relative amplitude from 1421 days following lesion, and returned to control value 28 days following lesion. In motor cortex both Wake and REM theta relative amplitude transiently increased 14 days following unilateral and bilateral NB lesion, and returned to control values 21 days after lesions. We demonstrated at functional level, for the first time, the topographically specific impact of NB cholinergic cortical afferent system dysregulation on sleep/wake states, REM and Wake EEG theta relative amplitude.",
journal = "Sleep and Biological Rhythms",
title = "Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder",
number = "2",
volume = "11",
pages = "243-115",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1027"
}

Šaponjić, J., Petrović, J., Kalauzi, A., Ćirić, J., Lazić, K., Radulovacki, M.,& Carley, D. W.. (2013). Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder. in Sleep and Biological Rhythms, 11(2), 243-115.
https://hdl.handle.net/21.15107/rcub_ibiss_1027

Šaponjić J, Petrović J, Kalauzi A, Ćirić J, Lazić K, Radulovacki M, Carley DW. Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder. in Sleep and Biological Rhythms. 2013;11(2):243-115.
https://hdl.handle.net/21.15107/rcub_ibiss_1027 .

Šaponjić, Jasna, Petrović, Jelena, Kalauzi, Aleksandar, Ćirić, Jelena, Lazić, Katarina, Radulovacki, Miodrag, Carley, David W, "Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder" in Sleep and Biological Rhythms, 11, no. 2 (2013):243-115,
https://hdl.handle.net/21.15107/rcub_ibiss_1027 .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Lazić, Katarina
AU  - Ćirić, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/949
AB  - In order to identify the differences for the onset and progression of functionally distinct cholinergic innervation disorders, we investigated the effect of bilateral nucleus basalis (NB) and pedunculopontine tegmental nucleus (PPT) lesions on sleep/wake states and electroencephalographic (EEG) microstructure in rats, chronically implanted for sleep recording. Bilateral NB lesion transiently altered Wake/NREM duration within the sensorimotor cortex, and Wake/REM duration within the motor cortex, while there was no change in the sleep/wake states distributions following the bilateral PPT lesion. Bilateral PPT lesion sustainably increased the Wake/REM and REM/Wake transitions followed by inconsistent dysregulation of the NREM/REM and REM/NREM transitions in sensorimotor cortex, but oppositely by their increment throughout four weeks in motor cortex. Bilateral NB lesion sustainably decreased the NREM/REM and REM/NREM transitions during four weeks in the sensorimotor cortex, but oppositely increased them in the motor cortex. We have shown that the sustained beta and gamma augmentation within the sensorimotor and motor cortex, and across all sleep/wake states, simultaneously with Wake delta amplitude attenuation only within the sensorimotor cortex, were the underlying EEG microstructure for the sleep/wake states-transitions structure disturbance following bilateral PPT lesion. In contrast, the bilateral NB lesion only augmented REM theta in sensorimotor cortex during three weeks. We have shown that the NB and PPT lesions induced differing, structure-related EEG microstructure and transition structure disturbances particularly expressed in motor cortex during NREM and REM sleep. We evidenced for the first time the different topographical expression of the functionally distinct cholinergic neuronal innervation impairment in rat. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Behavioural Brain Research
T1  - Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat
IS  - null
VL  - 256
SP  - 41
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_949
ER  - 

@article{
author = "Petrović, Jelena and Lazić, Katarina and Ćirić, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2013",
abstract = "In order to identify the differences for the onset and progression of functionally distinct cholinergic innervation disorders, we investigated the effect of bilateral nucleus basalis (NB) and pedunculopontine tegmental nucleus (PPT) lesions on sleep/wake states and electroencephalographic (EEG) microstructure in rats, chronically implanted for sleep recording. Bilateral NB lesion transiently altered Wake/NREM duration within the sensorimotor cortex, and Wake/REM duration within the motor cortex, while there was no change in the sleep/wake states distributions following the bilateral PPT lesion. Bilateral PPT lesion sustainably increased the Wake/REM and REM/Wake transitions followed by inconsistent dysregulation of the NREM/REM and REM/NREM transitions in sensorimotor cortex, but oppositely by their increment throughout four weeks in motor cortex. Bilateral NB lesion sustainably decreased the NREM/REM and REM/NREM transitions during four weeks in the sensorimotor cortex, but oppositely increased them in the motor cortex. We have shown that the sustained beta and gamma augmentation within the sensorimotor and motor cortex, and across all sleep/wake states, simultaneously with Wake delta amplitude attenuation only within the sensorimotor cortex, were the underlying EEG microstructure for the sleep/wake states-transitions structure disturbance following bilateral PPT lesion. In contrast, the bilateral NB lesion only augmented REM theta in sensorimotor cortex during three weeks. We have shown that the NB and PPT lesions induced differing, structure-related EEG microstructure and transition structure disturbances particularly expressed in motor cortex during NREM and REM sleep. We evidenced for the first time the different topographical expression of the functionally distinct cholinergic neuronal innervation impairment in rat. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Behavioural Brain Research",
title = "Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat",
number = "null",
volume = "256",
pages = "41-118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_949"
}

Petrović, J., Lazić, K., Ćirić, J., Kalauzi, A.,& Šaponjić, J.. (2013). Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat. in Behavioural Brain Research, 256(null), 41-118.
https://hdl.handle.net/21.15107/rcub_ibiss_949

Petrović J, Lazić K, Ćirić J, Kalauzi A, Šaponjić J. Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat. in Behavioural Brain Research. 2013;256(null):41-118.
https://hdl.handle.net/21.15107/rcub_ibiss_949 .

Petrović, Jelena, Lazić, Katarina, Ćirić, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat" in Behavioural Brain Research, 256, no. null (2013):41-118,
https://hdl.handle.net/21.15107/rcub_ibiss_949 .