TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Živanović, Ana
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Major, Tamara
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6548
AB  - Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease
IS  - 18
VL  - 24
DO  - 10.3390/ijms241814092
SP  - 14092
ER  - 

@article{
author = "Jovanović Macura, Irena and Živanović, Ana and Perović, Milka and Ćirić, Jelena and Major, Tamara and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease",
number = "18",
volume = "24",
doi = "10.3390/ijms241814092",
pages = "14092"
}

Jovanović Macura, I., Živanović, A., Perović, M., Ćirić, J., Major, T., Kanazir, S.,& Ivković, S.. (2023). The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences
Basel: MDPI., 24(18), 14092.
https://doi.org/10.3390/ijms241814092

Jovanović Macura I, Živanović A, Perović M, Ćirić J, Major T, Kanazir S, Ivković S. The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences. 2023;24(18):14092.
doi:10.3390/ijms241814092 .

Jovanović Macura, Irena, Živanović, Ana, Perović, Milka, Ćirić, Jelena, Major, Tamara, Kanazir, Selma, Ivković, Sanja, "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease" in International Journal of Molecular Sciences, 24, no. 18 (2023):14092,
https://doi.org/10.3390/ijms241814092 . .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5847
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5847
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5847"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5846
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5846
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5846"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

TY  - CONF
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Sobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5858
AB  - Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5858
ER  - 

@conference{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Sobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5858"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Sobajić, S., Kanazir, S.,& Ivković, S.. (2023). The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Sobajić S, Kanazir S, Ivković S. The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Sobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):66,
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5842
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5842
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5842"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):110,
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5843
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5843
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5843"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5843

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1756464622003723
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5174
AB  - The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.
T2  - Journal of Functional Foods
T1  - The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice
VL  - 99
DO  - 10.1016/j.jff.2022.105302
SP  - 105302
ER  - 

@article{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.",
journal = "Journal of Functional Foods",
title = "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice",
volume = "99",
doi = "10.1016/j.jff.2022.105302",
pages = "105302"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Šobajić, S., Kanazir, S.,& Ivković, S.. (2022). The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods, 99, 105302.
https://doi.org/10.1016/j.jff.2022.105302

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Šobajić S, Kanazir S, Ivković S. The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods. 2022;99:105302.
doi:10.1016/j.jff.2022.105302 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice" in Journal of Functional Foods, 99 (2022):105302,
https://doi.org/10.1016/j.jff.2022.105302 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Ivković, Sanja
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599456
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5172
AB  - Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101297
SP  - 1297
ER  - 

@article{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Ivković, Sanja and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101297",
pages = "1297"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Ivković, S., Kanazir, S.,& Perović, M.. (2022). Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences
Basel: MDPI., 12(10), 1297.
https://doi.org/10.3390/brainsci12101297

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Ivković S, Kanazir S, Perović M. Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences. 2022;12(10):1297.
doi:10.3390/brainsci12101297 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Ivković, Sanja, Kanazir, Selma, Perović, Milka, "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging" in Brain Sciences, 12, no. 10 (2022):1297,
https://doi.org/10.3390/brainsci12101297 . .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pešić, Vesna
AU  - Hofman, Katarina
AU  - Perović, Milka
AU  - Ćirić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5616
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti
T1  - Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести
SP  - 382
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5616
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pešić, Vesna and Hofman, Katarina and Perović, Milka and Ćirić, Jelena",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti, Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести",
pages = "382",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5616"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pešić, V., Hofman, K., Perović, M.,& Ćirić, J.. (2022). Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616

Milovanović N, Jovanović Macura I, Tešić V, Pešić V, Hofman K, Perović M, Ćirić J. Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pešić, Vesna, Hofman, Katarina, Perović, Milka, Ćirić, Jelena, "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):382,
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Hofman, Katarina
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5613
AB  - Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа.
AB  - Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti
T1  - Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести
SP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5613
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Hofman, Katarina and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа., Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti, Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести",
pages = "343",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5613"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Hofman, K., Kanazir, S.,& Perović, M.. (2022). Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Hofman K, Kanazir S, Perović M. Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Hofman, Katarina, Kanazir, Selma, Perović, Milka, "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):343,
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

TY  - CONF
AU  - Vukojević, Anđela
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
AU  - Jović, Milena
AU  - Jovanović Macura, Irena
AU  - Perović, Milka
AU  - Milanović, Desanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5325
AB  - Non­transmittable chronic diseases are largely driven by chronic inflammation, which can be connected to poor diet and toxic products of commensal gut microbiome.
Diet intervention can influence gut microbiota function and composition. Fermented foods are specifically known to have anti­inflammatory and immunomodulatory
properties, attributed to their high antioxidant content and lactic acid­producing bacteria (LAB) 
In this study we examined the effects of sauerkraut brine (SB) on physiological and behavioral responses to systemic inflammation induced by lipopolysaccharides
(LPS) in a mouse model.  C57BL/6 mice 90 postnatal days old were used in this study. They were divided into 2 groups and treated with either 150 ml of sauerkraut
brine and pasteurized sauerkraut brine (PSB) for 5 weeks (via oral gavage). Control animals (CON) were receiving an equivalent amount of water. During the final
week of treatment, animals received 5 injections of LPS (0.5 mg/kg, i.p.). Behavior of animals was assessed before and after LPS administration, using the open field
test, light­dark box, Y­maze, tail­suspension and rota­rod test. Analysis of pro­inflammatory brain cytokines has been performed subsequently via Western blot and
PCR. Food consumption and body weight were measured throughout the experiment.
SB and PSB treatments did not influence body weights and behavior compared to CON mice. LPS treatment led to the weight loss and decreased food intake in all
experimental groups. The fastest recovery and a reduced inflammatory response was detected in the SB group. Behavioral analysis revealed differences between three
groups in responses to the LPS challenge.
PB  - Federation of European Biochemical Societies
C3  - Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece
T1  - Protective role of fermented food in LPS-­induced inflammation in C57BL/6 mice
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5325
ER  - 

@conference{
author = "Vukojević, Anđela and Prvulović, Milica and Todorović, Smilja and Mladenović, Aleksandra and Jović, Milena and Jovanović Macura, Irena and Perović, Milka and Milanović, Desanka",
year = "2022",
abstract = "Non­transmittable chronic diseases are largely driven by chronic inflammation, which can be connected to poor diet and toxic products of commensal gut microbiome.
Diet intervention can influence gut microbiota function and composition. Fermented foods are specifically known to have anti­inflammatory and immunomodulatory
properties, attributed to their high antioxidant content and lactic acid­producing bacteria (LAB) 
In this study we examined the effects of sauerkraut brine (SB) on physiological and behavioral responses to systemic inflammation induced by lipopolysaccharides
(LPS) in a mouse model.  C57BL/6 mice 90 postnatal days old were used in this study. They were divided into 2 groups and treated with either 150 ml of sauerkraut
brine and pasteurized sauerkraut brine (PSB) for 5 weeks (via oral gavage). Control animals (CON) were receiving an equivalent amount of water. During the final
week of treatment, animals received 5 injections of LPS (0.5 mg/kg, i.p.). Behavior of animals was assessed before and after LPS administration, using the open field
test, light­dark box, Y­maze, tail­suspension and rota­rod test. Analysis of pro­inflammatory brain cytokines has been performed subsequently via Western blot and
PCR. Food consumption and body weight were measured throughout the experiment.
SB and PSB treatments did not influence body weights and behavior compared to CON mice. LPS treatment led to the weight loss and decreased food intake in all
experimental groups. The fastest recovery and a reduced inflammatory response was detected in the SB group. Behavioral analysis revealed differences between three
groups in responses to the LPS challenge.",
publisher = "Federation of European Biochemical Societies",
journal = "Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece",
title = "Protective role of fermented food in LPS-­induced inflammation in C57BL/6 mice",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5325"
}

Vukojević, A., Prvulović, M., Todorović, S., Mladenović, A., Jović, M., Jovanović Macura, I., Perović, M.,& Milanović, D.. (2022). Protective role of fermented food in LPS-­induced inflammation in C57BL/6 mice. in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece
Federation of European Biochemical Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5325

Vukojević A, Prvulović M, Todorović S, Mladenović A, Jović M, Jovanović Macura I, Perović M, Milanović D. Protective role of fermented food in LPS-­induced inflammation in C57BL/6 mice. in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5325 .

Vukojević, Anđela, Prvulović, Milica, Todorović, Smilja, Mladenović, Aleksandra, Jović, Milena, Jovanović Macura, Irena, Perović, Milka, Milanović, Desanka, "Protective role of fermented food in LPS-­induced inflammation in C57BL/6 mice" in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5325 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5187
AB  - Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice
DO  - 10.1002/2211-5463.13440
SP  - 132
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice",
doi = "10.1002/2211-5463.13440",
pages = "132"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Kanazir, S.,& Perović, M.. (2022). Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 132.
https://doi.org/10.1002/2211-5463.13440

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Kanazir S, Perović M. Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:132.
doi:10.1002/2211-5463.13440 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Kanazir, Selma, Perović, Milka, "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):132,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - CONF
AU  - Vukojević, Anđela
AU  - Prvulović, Milica
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
AU  - Jović, Milena
AU  - Jovanović-Macura, Irena
AU  - Perović, Milka
AU  - Milanović, Desanka
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5562
AB  - Non-communicable chronic diseases are largely driven by chronic inflammation, which can be in relation with poor diet and toxic products of commensal bacteria in guts. Diet intervention can change gut microbiota function and composition (Wastyk et al., 2021). The fermented foods containing microorganisms able to remodel host microbiota, can improve inflammatory status of the organism, including brain. Sauerkraut, produced by spontaneous fermentation of cabbage with lactic acid bacteria (LAB), is an important dietary ingredient, but studies of its effects are rather scarce (Zubaidah et al., 2020). Here, we aim to examine whether sauerkraut brine is able to change physiological and behavioral response to systemic inflammation in mice induced by lipopolysaccharides (LPS), a constituent of the Gram-negative bacteria cell wall (Kubera et al., 2016). 
Three-month-old C57BL/6 mice were given 150 l of sauerkraut brine (SB) or pasteurized sauerkraut brine (PSB) for 5 weeks by oral gavage. Control animals (CON) received the equivalent amount of water. During last week, animals were challenged by 5 injections of LPS (0.5 mg/kg, i.p.) Before and after LPS, behavior of animals was tested  by open field, light-dark box, Y-maze, tail-suspension and rota rod tests. Food consumption and body weight were measured throughout the experiment.
Quality analysis of in-home made sauerkraut produced by tradiotional spontaneous fermentation show that sauerkraut brine counts 2x105 colony forming units (CFU/ml) of LAB. SB and PSB treatments did not influence body weights and behavior compared to CON mice. LPS induced sick behavior characterized by weight loss and decreased food intake, where the fastest recovery was observed in the SB group. Behavioral analysis revealed similar response to LPS challenge between groups. However, further intra-group analysis and molecular screening is required to assess the possible subtle impact of sauerkraut on the mice behavior and immune status
PB  - Belgrade: Faculty of Chemistry: Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference: with international participation: Biochemical Insights into Molecular Mechanisms; 2021 Sep 24; Kragujevac, Serbia
T1  - Effects of fermented food on the body weight and behavior after repeated LPS administration in mice
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5562
ER  - 

@conference{
author = "Vukojević, Anđela and Prvulović, Milica and Todorović, Smilja and Mladenović, Aleksandra and Jović, Milena and Jovanović-Macura, Irena and Perović, Milka and Milanović, Desanka",
year = "2021",
abstract = "Non-communicable chronic diseases are largely driven by chronic inflammation, which can be in relation with poor diet and toxic products of commensal bacteria in guts. Diet intervention can change gut microbiota function and composition (Wastyk et al., 2021). The fermented foods containing microorganisms able to remodel host microbiota, can improve inflammatory status of the organism, including brain. Sauerkraut, produced by spontaneous fermentation of cabbage with lactic acid bacteria (LAB), is an important dietary ingredient, but studies of its effects are rather scarce (Zubaidah et al., 2020). Here, we aim to examine whether sauerkraut brine is able to change physiological and behavioral response to systemic inflammation in mice induced by lipopolysaccharides (LPS), a constituent of the Gram-negative bacteria cell wall (Kubera et al., 2016). 
Three-month-old C57BL/6 mice were given 150 l of sauerkraut brine (SB) or pasteurized sauerkraut brine (PSB) for 5 weeks by oral gavage. Control animals (CON) received the equivalent amount of water. During last week, animals were challenged by 5 injections of LPS (0.5 mg/kg, i.p.) Before and after LPS, behavior of animals was tested  by open field, light-dark box, Y-maze, tail-suspension and rota rod tests. Food consumption and body weight were measured throughout the experiment.
Quality analysis of in-home made sauerkraut produced by tradiotional spontaneous fermentation show that sauerkraut brine counts 2x105 colony forming units (CFU/ml) of LAB. SB and PSB treatments did not influence body weights and behavior compared to CON mice. LPS induced sick behavior characterized by weight loss and decreased food intake, where the fastest recovery was observed in the SB group. Behavioral analysis revealed similar response to LPS challenge between groups. However, further intra-group analysis and molecular screening is required to assess the possible subtle impact of sauerkraut on the mice behavior and immune status",
publisher = "Belgrade: Faculty of Chemistry: Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference: with international participation: Biochemical Insights into Molecular Mechanisms; 2021 Sep 24; Kragujevac, Serbia",
title = "Effects of fermented food on the body weight and behavior after repeated LPS administration in mice",
pages = "179",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5562"
}

Vukojević, A., Prvulović, M., Todorović, S., Mladenović, A., Jović, M., Jovanović-Macura, I., Perović, M.,& Milanović, D.. (2021). Effects of fermented food on the body weight and behavior after repeated LPS administration in mice. in Serbian Biochemical Society Tenth Conference: with international participation: Biochemical Insights into Molecular Mechanisms; 2021 Sep 24; Kragujevac, Serbia
Belgrade: Faculty of Chemistry: Serbian Biochemical Society., 179.
https://hdl.handle.net/21.15107/rcub_ibiss_5562

Vukojević A, Prvulović M, Todorović S, Mladenović A, Jović M, Jovanović-Macura I, Perović M, Milanović D. Effects of fermented food on the body weight and behavior after repeated LPS administration in mice. in Serbian Biochemical Society Tenth Conference: with international participation: Biochemical Insights into Molecular Mechanisms; 2021 Sep 24; Kragujevac, Serbia. 2021;:179.
https://hdl.handle.net/21.15107/rcub_ibiss_5562 .

Vukojević, Anđela, Prvulović, Milica, Todorović, Smilja, Mladenović, Aleksandra, Jović, Milena, Jovanović-Macura, Irena, Perović, Milka, Milanović, Desanka, "Effects of fermented food on the body weight and behavior after repeated LPS administration in mice" in Serbian Biochemical Society Tenth Conference: with international participation: Biochemical Insights into Molecular Mechanisms; 2021 Sep 24; Kragujevac, Serbia (2021):179,
https://hdl.handle.net/21.15107/rcub_ibiss_5562 .

TY  - JOUR
AU  - Tešić, Vesna
AU  - Ćirić, Jelena
AU  - Jovanović Macura, Irena
AU  - Zogović, Nevena
AU  - Milanović, Desanka
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2021
UR  - https://www.mdpi.com/2072-6643/13/12/4526
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8703853
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4760
AB  - Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.
IS  - 12
VL  - 13
DO  - 10.3390/nu13124526
SP  - 4526
ER  - 

@article{
author = "Tešić, Vesna and Ćirić, Jelena and Jovanović Macura, Irena and Zogović, Nevena and Milanović, Desanka and Kanazir, Selma and Perović, Milka",
year = "2021",
abstract = "Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.",
number = "12",
volume = "13",
doi = "10.3390/nu13124526",
pages = "4526"
}

Tešić, V., Ćirić, J., Jovanović Macura, I., Zogović, N., Milanović, D., Kanazir, S.,& Perović, M.. (2021). Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients
Basel: MDPI., 13(12), 4526.
https://doi.org/10.3390/nu13124526

Tešić V, Ćirić J, Jovanović Macura I, Zogović N, Milanović D, Kanazir S, Perović M. Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction.. in Nutrients. 2021;13(12):4526.
doi:10.3390/nu13124526 .

Tešić, Vesna, Ćirić, Jelena, Jovanović Macura, Irena, Zogović, Nevena, Milanović, Desanka, Kanazir, Selma, Perović, Milka, "Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction." in Nutrients, 13, no. 12 (2021):4526,
https://doi.org/10.3390/nu13124526 . .

TY  - JOUR
AU  - Ivković, Sanja
AU  - Jovanović Macura, Irena
AU  - Antonijević, Tijana
AU  - Kanazir, Selma
AU  - Henrique, Domingos
PY  - 2019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4389
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3648
AB  - Epidermal growth factor (EGF) signaling has been implicated in the regulation of the differentiation and proliferation of retinal progenitors. We assessed how different levels of EGF signaling, achieved either by increasing receptor expression or via addition of the exogenous ligand, or an increase in both, can affect the differentiation of progenitors in the first week of postnatal retinal development in the model system of retinal explants (REs). Proliferating progenitor cells in REs were infected with either the control CLV3/ESR-related peptide family (CLE)-green fluorescent protein (GFP)-or with EGF receptor (EGFR)-GFP-expressing retrovirus, and grown in the control medium or in the presence of exogenous EGF (10 ng/mL). The differentiation of infected cells into Muller glia (Sox9+), rod photoreceptors (rhodopsin+) and horizontal cells (calbindin+) was analyzed. In all the examined conditions, infected cells differentiated into Muller glia and rod photoreceptors that normally develop postnatally. Horizontal cells finished their development during the embryonic stages and progenitors infected with control-GFP virus did not differentiate into GFP+/calbindin-in either control or EGFsupplemented medium, however, cells infected with EGFR-GFP differentiated into horizontal cells (GFP+/calbindin+) in both culture conditions. These results imply that altering the levels of EGFR and/or the amount of the EGF ligand can overcome progenitor competence restriction.
T2  - Archives of Biological Sciences
T1  - Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina
IS  - 4
VL  - 71
DO  - 10.2298/abs190617054i
SP  - 711
EP  - 719
ER  - 

@article{
author = "Ivković, Sanja and Jovanović Macura, Irena and Antonijević, Tijana and Kanazir, Selma and Henrique, Domingos",
year = "2019",
abstract = "Epidermal growth factor (EGF) signaling has been implicated in the regulation of the differentiation and proliferation of retinal progenitors. We assessed how different levels of EGF signaling, achieved either by increasing receptor expression or via addition of the exogenous ligand, or an increase in both, can affect the differentiation of progenitors in the first week of postnatal retinal development in the model system of retinal explants (REs). Proliferating progenitor cells in REs were infected with either the control CLV3/ESR-related peptide family (CLE)-green fluorescent protein (GFP)-or with EGF receptor (EGFR)-GFP-expressing retrovirus, and grown in the control medium or in the presence of exogenous EGF (10 ng/mL). The differentiation of infected cells into Muller glia (Sox9+), rod photoreceptors (rhodopsin+) and horizontal cells (calbindin+) was analyzed. In all the examined conditions, infected cells differentiated into Muller glia and rod photoreceptors that normally develop postnatally. Horizontal cells finished their development during the embryonic stages and progenitors infected with control-GFP virus did not differentiate into GFP+/calbindin-in either control or EGFsupplemented medium, however, cells infected with EGFR-GFP differentiated into horizontal cells (GFP+/calbindin+) in both culture conditions. These results imply that altering the levels of EGFR and/or the amount of the EGF ligand can overcome progenitor competence restriction.",
journal = "Archives of Biological Sciences",
title = "Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina",
number = "4",
volume = "71",
doi = "10.2298/abs190617054i",
pages = "711-719"
}

Ivković, S., Jovanović Macura, I., Antonijević, T., Kanazir, S.,& Henrique, D.. (2019). Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina. in Archives of Biological Sciences, 71(4), 711-719.
https://doi.org/10.2298/abs190617054i

Ivković S, Jovanović Macura I, Antonijević T, Kanazir S, Henrique D. Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina. in Archives of Biological Sciences. 2019;71(4):711-719.
doi:10.2298/abs190617054i .

Ivković, Sanja, Jovanović Macura, Irena, Antonijević, Tijana, Kanazir, Selma, Henrique, Domingos, "Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina" in Archives of Biological Sciences, 71, no. 4 (2019):711-719,
https://doi.org/10.2298/abs190617054i . .