TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Batinić, Petar
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Carević, Tamara
AU  - Prijić, Željana
AU  - Marinković, Aleksandar
AU  - Marković, Tatjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6680
AB  - This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.
PB  - Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"
T2  - Lekovite sirovine
T1  - Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia
IS  - 1
VL  - 43
DO  - 10.61652/leksir2343e159B
SP  - e159
ER  - 

@article{
author = "Batinić, Petar and Čutović, Natalija and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Carević, Tamara and Prijić, Željana and Marinković, Aleksandar and Marković, Tatjana",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.",
publisher = "Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"",
journal = "Lekovite sirovine",
title = "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia",
number = "1",
volume = "43",
doi = "10.61652/leksir2343e159B",
pages = "e159"
}

Batinić, P., Čutović, N., Jovanović, A., Stojković, D., Zengin, G., Carević, T., Prijić, Ž., Marinković, A.,& Marković, T.. (2023). Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine
Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"., 43(1), e159.
https://doi.org/10.61652/leksir2343e159B

Batinić P, Čutović N, Jovanović A, Stojković D, Zengin G, Carević T, Prijić Ž, Marinković A, Marković T. Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine. 2023;43(1):e159.
doi:10.61652/leksir2343e159B .

Batinić, Petar, Čutović, Natalija, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Carević, Tamara, Prijić, Željana, Marinković, Aleksandar, Marković, Tatjana, "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia" in Lekovite sirovine, 43, no. 1 (2023):e159,
https://doi.org/10.61652/leksir2343e159B . .

TY  - JOUR
AU  - Marković, Tatjana
AU  - Čutović, Natalija
AU  - Carević, Tamara
AU  - Gašić, Uroš
AU  - Stojković, Dejan
AU  - Xue, Jingqi
AU  - Jovanović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6072
AB  - Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts
IS  - 14
VL  - 24
DO  - 10.3390/ijms241411764
SP  - 11764
ER  - 

@article{
author = "Marković, Tatjana and Čutović, Natalija and Carević, Tamara and Gašić, Uroš and Stojković, Dejan and Xue, Jingqi and Jovanović, Aleksandra",
year = "2023",
abstract = "Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts",
number = "14",
volume = "24",
doi = "10.3390/ijms241411764",
pages = "11764"
}

Marković, T., Čutović, N., Carević, T., Gašić, U., Stojković, D., Xue, J.,& Jovanović, A.. (2023). Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences
Basel: MDPI., 24(14), 11764.
https://doi.org/10.3390/ijms241411764

Marković T, Čutović N, Carević T, Gašić U, Stojković D, Xue J, Jovanović A. Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences. 2023;24(14):11764.
doi:10.3390/ijms241411764 .

Marković, Tatjana, Čutović, Natalija, Carević, Tamara, Gašić, Uroš, Stojković, Dejan, Xue, Jingqi, Jovanović, Aleksandra, "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts" in International Journal of Molecular Sciences, 24, no. 14 (2023):11764,
https://doi.org/10.3390/ijms241411764 . .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Marković, Tatjana
AU  - Sanković Babić, Snežana
AU  - Barros, Lillian
AU  - Calhelha, Ricardo
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/10412905.2022.2107103
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5101
AB  - The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.
PB  - Taylor and Francis Ltd.
T2  - Journal of Essential Oil Research
T1  - An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities
IS  - 6
VL  - 34
DO  - 10.1080/10412905.2022.2107103
SP  - 533
EP  - 543
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Marković, Tatjana and Sanković Babić, Snežana and Barros, Lillian and Calhelha, Ricardo and Soković, Marina and Ćirić, Ana",
year = "2022",
abstract = "The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Essential Oil Research",
title = "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities",
number = "6",
volume = "34",
doi = "10.1080/10412905.2022.2107103",
pages = "533-543"
}

Kostić, M., Ivanov, M., Marković, T., Sanković Babić, S., Barros, L., Calhelha, R., Soković, M.,& Ćirić, A.. (2022). An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research
Taylor and Francis Ltd.., 34(6), 533-543.
https://doi.org/10.1080/10412905.2022.2107103

Kostić M, Ivanov M, Marković T, Sanković Babić S, Barros L, Calhelha R, Soković M, Ćirić A. An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research. 2022;34(6):533-543.
doi:10.1080/10412905.2022.2107103 .

Kostić, Marina, Ivanov, Marija, Marković, Tatjana, Sanković Babić, Snežana, Barros, Lillian, Calhelha, Ricardo, Soković, Marina, Ćirić, Ana, "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities" in Journal of Essential Oil Research, 34, no. 6 (2022):533-543,
https://doi.org/10.1080/10412905.2022.2107103 . .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Kostić, Marina
AU  - Gašić, Uroš
AU  - Prijić, Željana
AU  - Ren, Xiuxia
AU  - Lukić, Milan
AU  - Bugarski, Branko
PY  - 2022
UR  - https://www.mdpi.com/1424-8247/15/12/1537
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5358
AB  - Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia
IS  - 12
VL  - 15
DO  - 10.3390/ph15121537
SP  - 1537
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Kostić, Marina and Gašić, Uroš and Prijić, Željana and Ren, Xiuxia and Lukić, Milan and Bugarski, Branko",
year = "2022",
abstract = "Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia",
number = "12",
volume = "15",
doi = "10.3390/ph15121537",
pages = "1537"
}

Čutović, N., Marković, T., Kostić, M., Gašić, U., Prijić, Ž., Ren, X., Lukić, M.,& Bugarski, B.. (2022). Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals
Basel: MDPI., 15(12), 1537.
https://doi.org/10.3390/ph15121537

Čutović N, Marković T, Kostić M, Gašić U, Prijić Ž, Ren X, Lukić M, Bugarski B. Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals. 2022;15(12):1537.
doi:10.3390/ph15121537 .

Čutović, Natalija, Marković, Tatjana, Kostić, Marina, Gašić, Uroš, Prijić, Željana, Ren, Xiuxia, Lukić, Milan, Bugarski, Branko, "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia" in Pharmaceuticals, 15, no. 12 (2022):1537,
https://doi.org/10.3390/ph15121537 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Mitić-Ćulafić, Dragana
AU  - Dukanović, Stefana
AU  - Krstić, Aleksandar
AU  - Pavlica, Dušan
AU  - Vlajić, Tamara
AU  - Marković, Dejan
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/0972060X.2022.2040386
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4944
AB  - The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.
PB  - Abingdon: Taylor & Francis Ltd.
T2  - Journal of Essential Oil-Bearing Plants
T1  - Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes
IS  - 1
VL  - 25
DO  - 10.1080/0972060X.2022.2040386
SP  - 111
EP  - 125
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Dukanović, Stefana and Krstić, Aleksandar and Pavlica, Dušan and Vlajić, Tamara and Marković, Dejan",
year = "2022",
abstract = "The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.",
publisher = "Abingdon: Taylor & Francis Ltd.",
journal = "Journal of Essential Oil-Bearing Plants",
title = "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes",
number = "1",
volume = "25",
doi = "10.1080/0972060X.2022.2040386",
pages = "111-125"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Mitić-Ćulafić, D., Dukanović, S., Krstić, A., Pavlica, D., Vlajić, T.,& Marković, D.. (2022). Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants
Abingdon: Taylor & Francis Ltd.., 25(1), 111-125.
https://doi.org/10.1080/0972060X.2022.2040386

Marinković J, Marković T, Nikolić B, Ćirić A, Mitić-Ćulafić D, Dukanović S, Krstić A, Pavlica D, Vlajić T, Marković D. Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants. 2022;25(1):111-125.
doi:10.1080/0972060X.2022.2040386 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Dukanović, Stefana, Krstić, Aleksandar, Pavlica, Dušan, Vlajić, Tamara, Marković, Dejan, "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes" in Journal of Essential Oil-Bearing Plants, 25, no. 1 (2022):111-125,
https://doi.org/10.1080/0972060X.2022.2040386 . .

TY  - CONF
AU  - Nikolić, Biljana
AU  - Marković, Dejan
AU  - Marković, Tatjana
AU  - Mitić-Ćulafić, Dragana
AU  - Vuletić, Stefana
AU  - Cvetković, Stefana
AU  - Ćirić, Ana
AU  - Marinković, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5584
AB  - Mикробиота усне дупље обухвата више стотина/хиљада врста са примарном
функцијом заштите организма од колонизације патогенима. Међутим, ако
осетљиви екосистем усне дупље изађе из равнотеже, услед пада имунитета и/или
пренамножености микроорганизама у биофилму (денталном плаку), развијају се
болести зуба (каријес, инфекције зубне пулпе) или периодонтална обољења, што
чак може угрозити системско здравље. Имајући то у виду, лечење зубних
инфекција је од изузетног значаја, а потреба за развојем нових антимикробних
агенаса/процедура евидентна. Пошто су етарска уља (ЕУ) позната по снажном
антибактеријском/антибиофилм ефекту, фокусирали смо се на истраживање
њихове потенцијалне примене у лечењу инфекција коренских канала зуба. Из
инфицираних канала изоловани су бројни факултативни и стриктни анаероби,1 али
је истраживање усмерено на Enterococcus faecalis, као најчешћег узрочника
перзистентних/рекурентних инфекција. Иако смо антибактеријски ефекат
детектовали за бројна ЕУ, детаљна истраживања су спроведена са уљима Thymus
zygis, Cymbopogon martinii2 и C. citratus3. Адекватна испорука ЕУ у коренски канал
подразумевала је формулацију микро- и наноемулзија. Истраживање је спроведено
in vitro и на моно- (E. faecalis) и мултиспецијском биофилму у каналима
екстрахованих зуба. У случају C. martinii оптимизована је и процедура припреме
ефикасних наноемулзија. Добијени резултати су обећавајући: емулзије ЕУ се
успешно могу користити као допунска терапија инфицираних коренских канала
зуба.
AB  - Mikrobiota usne duplje obuhvata više stotina/hiljada vrsta sa primarnom funkcijom zaštite organizma od kolonizacije patogenima. Međutim, ako osetljivi ekosistem usne duplje izađe iz ravnoteže, usled pada imuniteta i/ili prenamnoženosti mikroorganizama u biofilmu (dentalnom plaku), razvijaju se bolesti zuba (karijes, infekcije zubne pulpe) ili periodontalna oboljenja, što čak može ugroziti sistemsko zdravlje. Imajući to u vidu, lečenje zubnih infekcija je od izuzetnog značaja, a potreba za razvojem novih antimikrobnih agenasa/procedura evidentna. Pošto su etarska ulja (EU) poznata po snažnom antibakterijskom/antibiofilm efektu, fokusirali smo se na istraživanje njihove potencijalne primene u lečenju infekcija korenskih kanala zuba. Iz inficiranih kanala izolovani su brojni fakultativni i striktni anaerobi,1 ali je istraživanje usmereno na Enterococcus faecalis, kao najčešćeg uzročnika perzistentnih/rekurentnih infekcija. Iako smo antibakterijski efekat detektovali za brojna EU, detaljna istraživanja su sprovedena sa uljima Thymus zygis, Cymbopogon martinii2 i C. citratus3. Adekvatna isporuka EU u korenski kanal podrazumevala je formulaciju mikro- i nanoemulzija. Istraživanje je sprovedeno in vitro i na mono- (E. faecalis) i multispecijskom biofilmu u kanalima ekstrahovanih zuba. U slučaju C. martinii optimizovana je i procedura pripreme efikasnih nanoemulzija. Dobijeni rezultati su obećavajući: emulzije EU se uspešno mogu koristiti kao dopunska terapija inficiranih korenskih kanala zuba.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba
T1  - Могућности примене антибактеријског и антибиофилмног потенцијала етарских уља у лечењу инфекција зуба
SP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5584
ER  - 

@conference{
author = "Nikolić, Biljana and Marković, Dejan and Marković, Tatjana and Mitić-Ćulafić, Dragana and Vuletić, Stefana and Cvetković, Stefana and Ćirić, Ana and Marinković, Jelena",
year = "2022",
abstract = "Mикробиота усне дупље обухвата више стотина/хиљада врста са примарном
функцијом заштите организма од колонизације патогенима. Међутим, ако
осетљиви екосистем усне дупље изађе из равнотеже, услед пада имунитета и/или
пренамножености микроорганизама у биофилму (денталном плаку), развијају се
болести зуба (каријес, инфекције зубне пулпе) или периодонтална обољења, што
чак може угрозити системско здравље. Имајући то у виду, лечење зубних
инфекција је од изузетног значаја, а потреба за развојем нових антимикробних
агенаса/процедура евидентна. Пошто су етарска уља (ЕУ) позната по снажном
антибактеријском/антибиофилм ефекту, фокусирали смо се на истраживање
њихове потенцијалне примене у лечењу инфекција коренских канала зуба. Из
инфицираних канала изоловани су бројни факултативни и стриктни анаероби,1 али
је истраживање усмерено на Enterococcus faecalis, као најчешћег узрочника
перзистентних/рекурентних инфекција. Иако смо антибактеријски ефекат
детектовали за бројна ЕУ, детаљна истраживања су спроведена са уљима Thymus
zygis, Cymbopogon martinii2 и C. citratus3. Адекватна испорука ЕУ у коренски канал
подразумевала је формулацију микро- и наноемулзија. Истраживање је спроведено
in vitro и на моно- (E. faecalis) и мултиспецијском биофилму у каналима
екстрахованих зуба. У случају C. martinii оптимизована је и процедура припреме
ефикасних наноемулзија. Добијени резултати су обећавајући: емулзије ЕУ се
успешно могу користити као допунска терапија инфицираних коренских канала
зуба., Mikrobiota usne duplje obuhvata više stotina/hiljada vrsta sa primarnom funkcijom zaštite organizma od kolonizacije patogenima. Međutim, ako osetljivi ekosistem usne duplje izađe iz ravnoteže, usled pada imuniteta i/ili prenamnoženosti mikroorganizama u biofilmu (dentalnom plaku), razvijaju se bolesti zuba (karijes, infekcije zubne pulpe) ili periodontalna oboljenja, što čak može ugroziti sistemsko zdravlje. Imajući to u vidu, lečenje zubnih infekcija je od izuzetnog značaja, a potreba za razvojem novih antimikrobnih agenasa/procedura evidentna. Pošto su etarska ulja (EU) poznata po snažnom antibakterijskom/antibiofilm efektu, fokusirali smo se na istraživanje njihove potencijalne primene u lečenju infekcija korenskih kanala zuba. Iz inficiranih kanala izolovani su brojni fakultativni i striktni anaerobi,1 ali je istraživanje usmereno na Enterococcus faecalis, kao najčešćeg uzročnika perzistentnih/rekurentnih infekcija. Iako smo antibakterijski efekat detektovali za brojna EU, detaljna istraživanja su sprovedena sa uljima Thymus zygis, Cymbopogon martinii2 i C. citratus3. Adekvatna isporuka EU u korenski kanal podrazumevala je formulaciju mikro- i nanoemulzija. Istraživanje je sprovedeno in vitro i na mono- (E. faecalis) i multispecijskom biofilmu u kanalima ekstrahovanih zuba. U slučaju C. martinii optimizovana je i procedura pripreme efikasnih nanoemulzija. Dobijeni rezultati su obećavajući: emulzije EU se uspešno mogu koristiti kao dopunska terapija inficiranih korenskih kanala zuba.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba, Могућности примене антибактеријског и антибиофилмног потенцијала етарских уља у лечењу инфекција зуба",
pages = "4",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5584"
}

Nikolić, B., Marković, D., Marković, T., Mitić-Ćulafić, D., Vuletić, S., Cvetković, S., Ćirić, A.,& Marinković, J.. (2022). Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 4.
https://hdl.handle.net/21.15107/rcub_ibiss_5584

Nikolić B, Marković D, Marković T, Mitić-Ćulafić D, Vuletić S, Cvetković S, Ćirić A, Marinković J. Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:4.
https://hdl.handle.net/21.15107/rcub_ibiss_5584 .

Nikolić, Biljana, Marković, Dejan, Marković, Tatjana, Mitić-Ćulafić, Dragana, Vuletić, Stefana, Cvetković, Stefana, Ćirić, Ana, Marinković, Jelena, "Mogućnosti primene antibakterijskog i antibiofilmnog potencijala etarskih ulja u lečenju infekcija zuba" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):4,
https://hdl.handle.net/21.15107/rcub_ibiss_5584 .

TY  - CONF
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4378
AB  - Essential oils (EOs) are potent antibacterial agents that have numerous applications in different fields, such 
as traditional medicine, pharmacy and cosmetics, food and beverage industry. However, their use in 
dentistry is limited and not adequately explored. In order to investigate the potential to expand their 
applications in dentistry, we screened for antibacterial and antibiofilm activity of selected EOs toward 
Streptococcus salivarius dental isolates. Selected EOs were originated from Pelargonium roseum,
Pelargonium graveolens, Leptospermum petersonii and Eucalyptus citriodora. In order to take insight into 
the variation in sensitivity among bacterial strains of different origin, both isolates from symptomatic and 
asymptomatic root canal infections, as well as the reference S. salivarius ATCC 9222 strain, were involved 
in this study. EOs were chemically characterized by GC/MS-FID analysis. Microdilution assay, was used 
to determine minimal inhibitory concentrations (MICs). Crystal violet (CV) assay was provided to explore 
the potential to disturb biofilm formation. Statistical analysis performed by One Way ANOVA was used to 
compare sensitivity between tested strains.
Chemical characterization revealed that all EOs were abundant in oxygenated monoterpenes (76.8 – 92.4% 
composition). Furthermore, the content of sesquiterpene hydrocarbons was relatively high in P. roseum and 
P. graveolens EOs (12.3% and 16.7%, respectively). The major constituents of Pelargonium oils were 
citronellol, geraniol, citronellyl formate and geranyl formate, contributing together with 71.3% and 57.1% 
of total P. roseum and P. graveolens EOs, respectively. EO of L. petersonii was abundant with following 
dominant constituents: geranial, neral, citronellal, citronellol and isopulegol (accounting for 81.2% of the 
total EO). Some of these constituents, namely citronellal, citronellol and isopulegol, were also dominant in 
E. citriodora oil, constituting 84.8% of its composition. MIC assay demonstrated that referent strain was 
the most sensitive, while the strains isolated from symptomatic infections were highly resistant. Considering 
all tested strains, the comparison of EOs antibacterial activity pointed out the following order, calculated in 
mg mL-1
: E. citriodora (MIC values 0.08±0.03 – 5.42±1.71) > L. petersonii (MIC values 0.53±0.00 –
4.74±0.64) > P. graveolens (MIC values 0.36±0.15 – 5.54±0.00) > P. roseum (MIC values 1.50±0.68 –
13.77±0.69). The inhibition of biofilm formation by EOs was also strain specific; clinical isolates were less 
sensitive than the reference strain (the ranges of biofilm inhibition were 24%-75% and 84%-89%, 
respectively). Similarly as in the microdilution assay, the E. citriodora EO was the most potent biofilm 
inhibitor. 
Taking into account all obtained results, Eucalyptus citriodora EO proved promising antibacterial and 
antibiofilm activity and could be recommended for further investigation as alternative antimicrobial in 
dental practice.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - New insights into anti-streptococcal potential of selected essential oils relevant to dentistry
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4378
ER  - 

@conference{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Soković, Marina and Marković, Dejan",
year = "2021",
abstract = "Essential oils (EOs) are potent antibacterial agents that have numerous applications in different fields, such 
as traditional medicine, pharmacy and cosmetics, food and beverage industry. However, their use in 
dentistry is limited and not adequately explored. In order to investigate the potential to expand their 
applications in dentistry, we screened for antibacterial and antibiofilm activity of selected EOs toward 
Streptococcus salivarius dental isolates. Selected EOs were originated from Pelargonium roseum,
Pelargonium graveolens, Leptospermum petersonii and Eucalyptus citriodora. In order to take insight into 
the variation in sensitivity among bacterial strains of different origin, both isolates from symptomatic and 
asymptomatic root canal infections, as well as the reference S. salivarius ATCC 9222 strain, were involved 
in this study. EOs were chemically characterized by GC/MS-FID analysis. Microdilution assay, was used 
to determine minimal inhibitory concentrations (MICs). Crystal violet (CV) assay was provided to explore 
the potential to disturb biofilm formation. Statistical analysis performed by One Way ANOVA was used to 
compare sensitivity between tested strains.
Chemical characterization revealed that all EOs were abundant in oxygenated monoterpenes (76.8 – 92.4% 
composition). Furthermore, the content of sesquiterpene hydrocarbons was relatively high in P. roseum and 
P. graveolens EOs (12.3% and 16.7%, respectively). The major constituents of Pelargonium oils were 
citronellol, geraniol, citronellyl formate and geranyl formate, contributing together with 71.3% and 57.1% 
of total P. roseum and P. graveolens EOs, respectively. EO of L. petersonii was abundant with following 
dominant constituents: geranial, neral, citronellal, citronellol and isopulegol (accounting for 81.2% of the 
total EO). Some of these constituents, namely citronellal, citronellol and isopulegol, were also dominant in 
E. citriodora oil, constituting 84.8% of its composition. MIC assay demonstrated that referent strain was 
the most sensitive, while the strains isolated from symptomatic infections were highly resistant. Considering 
all tested strains, the comparison of EOs antibacterial activity pointed out the following order, calculated in 
mg mL-1
: E. citriodora (MIC values 0.08±0.03 – 5.42±1.71) > L. petersonii (MIC values 0.53±0.00 –
4.74±0.64) > P. graveolens (MIC values 0.36±0.15 – 5.54±0.00) > P. roseum (MIC values 1.50±0.68 –
13.77±0.69). The inhibition of biofilm formation by EOs was also strain specific; clinical isolates were less 
sensitive than the reference strain (the ranges of biofilm inhibition were 24%-75% and 84%-89%, 
respectively). Similarly as in the microdilution assay, the E. citriodora EO was the most potent biofilm 
inhibitor. 
Taking into account all obtained results, Eucalyptus citriodora EO proved promising antibacterial and 
antibiofilm activity and could be recommended for further investigation as alternative antimicrobial in 
dental practice.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "New insights into anti-streptococcal potential of selected essential oils relevant to dentistry",
pages = "131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4378"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Soković, M.,& Marković, D.. (2021). New insights into anti-streptococcal potential of selected essential oils relevant to dentistry. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 131.
https://hdl.handle.net/21.15107/rcub_ibiss_4378

Marinković J, Marković T, Nikolić B, Ćirić A, Soković M, Marković D. New insights into anti-streptococcal potential of selected essential oils relevant to dentistry. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:131.
https://hdl.handle.net/21.15107/rcub_ibiss_4378 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Soković, Marina, Marković, Dejan, "New insights into anti-streptococcal potential of selected essential oils relevant to dentistry" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):131,
https://hdl.handle.net/21.15107/rcub_ibiss_4378 .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Soldatović, Ivan
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Marković, Dejan
PY  - 2021
UR  - https://www.tandfonline.com/doi/abs/10.1080/0972060X.2021.1899059
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4245
AB  - The study aimed to investigate the potency of Leptospermum petersonii, Eucalyptus citriodora, Pelargonium graveolens, and Pelargonium roseum essential oils (EOs) against Streptococcus sanguinis and Streptococcus salivarius dental isolates. To examine the variation in sensitivity among strains of different origin, isolates from symptomatic and asymptomatic root canal infections, as well as the reference, strains S. sanguinis ATCC 10556 and S. salivarius ATCC 9222 were used in the study. EOs were chemically characterised (GC/MS). Their antibacterial and antibiofilm properties were detected by microdilution and CV assay respectively. Statistical analysis was performed by One way ANOVA. Examined bacterial strains, regardless clinical or ATCC, exhibited the highest sensitivity to L. petersonii and E. citriodora EOs (MIC ranged 0.53±0.00 - 5.88±1.27 mg/ml and 0.08±0.03 - 9.37±1.25 mg/ml respectively), while two other EOs were less efficient. Comparison of MIC values showed that sensitivity of the isolates recovered from symptomatic infections was the least, while the susceptibility of asymptomatic isolates and especially of reference strains were higher. EO inhibition of biofilm formation differed between clinical isolates of S. salivarius and their reference strain. Inhibition was higher in the case of S. salivarius ATCC 9222 (84 %-89 %) in comparison to that of clinical isolates (24 %-75 %). Regardless of the strains tested, E. citriodora exhibited the highest antibiofilm potential. To conclude: L. petersonii induced notable antibacterial potential, while E. citriodora EO proved promising antibacterial and antibiofilm activity. Due to the variable sensitivity of the isolates to EOs, it is suggested to screen activity on a large number of strains, especially from symptomatic infections.
PB  - Informa UK Limited
T2  - Journal of Essential Oil Bearing Plants
T1  - Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates
IS  - 2
VL  - 24
DO  - 10.1080/0972060x.2021.1899059
SP  - 304
EP  - 316
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Soldatović, Ivan and Ivanov, Marija and Ćirić, Ana and Soković, Marina and Marković, Dejan",
year = "2021",
abstract = "The study aimed to investigate the potency of Leptospermum petersonii, Eucalyptus citriodora, Pelargonium graveolens, and Pelargonium roseum essential oils (EOs) against Streptococcus sanguinis and Streptococcus salivarius dental isolates. To examine the variation in sensitivity among strains of different origin, isolates from symptomatic and asymptomatic root canal infections, as well as the reference, strains S. sanguinis ATCC 10556 and S. salivarius ATCC 9222 were used in the study. EOs were chemically characterised (GC/MS). Their antibacterial and antibiofilm properties were detected by microdilution and CV assay respectively. Statistical analysis was performed by One way ANOVA. Examined bacterial strains, regardless clinical or ATCC, exhibited the highest sensitivity to L. petersonii and E. citriodora EOs (MIC ranged 0.53±0.00 - 5.88±1.27 mg/ml and 0.08±0.03 - 9.37±1.25 mg/ml respectively), while two other EOs were less efficient. Comparison of MIC values showed that sensitivity of the isolates recovered from symptomatic infections was the least, while the susceptibility of asymptomatic isolates and especially of reference strains were higher. EO inhibition of biofilm formation differed between clinical isolates of S. salivarius and their reference strain. Inhibition was higher in the case of S. salivarius ATCC 9222 (84 %-89 %) in comparison to that of clinical isolates (24 %-75 %). Regardless of the strains tested, E. citriodora exhibited the highest antibiofilm potential. To conclude: L. petersonii induced notable antibacterial potential, while E. citriodora EO proved promising antibacterial and antibiofilm activity. Due to the variable sensitivity of the isolates to EOs, it is suggested to screen activity on a large number of strains, especially from symptomatic infections.",
publisher = "Informa UK Limited",
journal = "Journal of Essential Oil Bearing Plants",
title = "Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates",
number = "2",
volume = "24",
doi = "10.1080/0972060x.2021.1899059",
pages = "304-316"
}

Marinković, J., Marković, T., Nikolić, B., Soldatović, I., Ivanov, M., Ćirić, A., Soković, M.,& Marković, D.. (2021). Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates. in Journal of Essential Oil Bearing Plants
Informa UK Limited., 24(2), 304-316.
https://doi.org/10.1080/0972060x.2021.1899059

Marinković J, Marković T, Nikolić B, Soldatović I, Ivanov M, Ćirić A, Soković M, Marković D. Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates. in Journal of Essential Oil Bearing Plants. 2021;24(2):304-316.
doi:10.1080/0972060x.2021.1899059 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Soldatović, Ivan, Ivanov, Marija, Ćirić, Ana, Soković, Marina, Marković, Dejan, "Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates" in Journal of Essential Oil Bearing Plants, 24, no. 2 (2021):304-316,
https://doi.org/10.1080/0972060x.2021.1899059 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Nikolić, Biljana
AU  - Marković, Tatjana
AU  - Radulović, Milena
AU  - Ilić, Jugoslav
AU  - Bošković, Marko
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4360
AB  - The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus
oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and an tibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization
of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination
of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by
2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm
efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU count ing assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed
biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential.
Both substances induced some antioxidant activity. Conclusion: Results encourage further research with
the aim of application of the nanoemulsion in dental practice.
PB  - London: Future Medicine Ltd.
T2  - Future Microbiology
T1  - Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm
IS  - 12
VL  - 16
DO  - 10.2217/fmb-2021-0081
SP  - 907
EP  - 918
ER  - 

@article{
author = "Marinković, Jelena and Nikolić, Biljana and Marković, Tatjana and Radulović, Milena and Ilić, Jugoslav and Bošković, Marko and Ćirić, Ana and Marković, Dejan",
year = "2021",
abstract = "The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus
oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and an tibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization
of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination
of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by
2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm
efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU count ing assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed
biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential.
Both substances induced some antioxidant activity. Conclusion: Results encourage further research with
the aim of application of the nanoemulsion in dental practice.",
publisher = "London: Future Medicine Ltd.",
journal = "Future Microbiology",
title = "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm",
number = "12",
volume = "16",
doi = "10.2217/fmb-2021-0081",
pages = "907-918"
}

Marinković, J., Nikolić, B., Marković, T., Radulović, M., Ilić, J., Bošković, M., Ćirić, A.,& Marković, D.. (2021). Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology
London: Future Medicine Ltd.., 16(12), 907-918.
https://doi.org/10.2217/fmb-2021-0081

Marinković J, Nikolić B, Marković T, Radulović M, Ilić J, Bošković M, Ćirić A, Marković D. Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology. 2021;16(12):907-918.
doi:10.2217/fmb-2021-0081 .

Marinković, Jelena, Nikolić, Biljana, Marković, Tatjana, Radulović, Milena, Ilić, Jugoslav, Bošković, Marko, Ćirić, Ana, Marković, Dejan, "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm" in Future Microbiology, 16, no. 12 (2021):907-918,
https://doi.org/10.2217/fmb-2021-0081 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Šešlija Jovanović, Darka
AU  - Kostić, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4504
AB  - The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood
forests. In the search for an environmentally safe means of its control, we assessed the impact
of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise
Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality,
molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with
commercial insecticide NeemAzal®-T/S (neem). The main compounds in the Eos were trans-anethole
in anise; carvone, limonene, and  -phellandrene in dill; and trans-anethole and fenchone in fennel
seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more
toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%,
anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs
were more effective in reducing growth rate, approximate digestibility and efficiency of conversion
of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and
metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from
both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used
for gypsy moth control.
PB  - Basel: MDPI
T2  - Plants
T1  - Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control
IS  - 10
VL  - 10
DO  - 10.3390/plants10102194
SP  - 2194
ER  - 

@article{
author = "Kostić, Igor and Lazarević, Jelica and Šešlija Jovanović, Darka and Kostić, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood
forests. In the search for an environmentally safe means of its control, we assessed the impact
of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise
Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality,
molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with
commercial insecticide NeemAzal®-T/S (neem). The main compounds in the Eos were trans-anethole
in anise; carvone, limonene, and  -phellandrene in dill; and trans-anethole and fenchone in fennel
seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more
toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%,
anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs
were more effective in reducing growth rate, approximate digestibility and efficiency of conversion
of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and
metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from
both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used
for gypsy moth control.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control",
number = "10",
volume = "10",
doi = "10.3390/plants10102194",
pages = "2194"
}

Kostić, I., Lazarević, J., Šešlija Jovanović, D., Kostić, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants
Basel: MDPI., 10(10), 2194.
https://doi.org/10.3390/plants10102194

Kostić I, Lazarević J, Šešlija Jovanović D, Kostić M, Marković T, Milanović S. Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants. 2021;10(10):2194.
doi:10.3390/plants10102194 .

Kostić, Igor, Lazarević, Jelica, Šešlija Jovanović, Darka, Kostić, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control" in Plants, 10, no. 10 (2021):2194,
https://doi.org/10.3390/plants10102194 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Calhelha, Ricardo
AU  - Fernandes, Ângela
AU  - Ferreira, Isabel
AU  - Stojković, Dejan
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51392100096N
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4761
AB  - Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.
PB  - Belgrade: Serbian Chemical Socitey
T2  - Journal of the Serbian Chemical Society
T1  - Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute
IS  - 12
VL  - 86
DO  - 10.2298/JSC210729096N
SP  - 1291
EP  - 1303
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Marković, Dejan and Calhelha, Ricardo and Fernandes, Ângela and Ferreira, Isabel and Stojković, Dejan and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2021",
abstract = "Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.",
publisher = "Belgrade: Serbian Chemical Socitey",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute",
number = "12",
volume = "86",
doi = "10.2298/JSC210729096N",
pages = "1291-1303"
}

Nikolić, M., Marković, T., Marković, D., Calhelha, R., Fernandes, Â., Ferreira, I., Stojković, D., Ćirić, A., Glamočlija, J.,& Soković, M.. (2021). Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Socitey., 86(12), 1291-1303.
https://doi.org/10.2298/JSC210729096N

Nikolić M, Marković T, Marković D, Calhelha R, Fernandes Â, Ferreira I, Stojković D, Ćirić A, Glamočlija J, Soković M. Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society. 2021;86(12):1291-1303.
doi:10.2298/JSC210729096N .

Nikolić, Miloš, Marković, Tatjana, Marković, Dejan, Calhelha, Ricardo, Fernandes, Ângela, Ferreira, Isabel, Stojković, Dejan, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute" in Journal of the Serbian Chemical Society, 86, no. 12 (2021):1291-1303,
https://doi.org/10.2298/JSC210729096N . .

TY  - DATA
AU  - Kostić, igor
AU  - Lazarević, Jelica
AU  - Šešlija Jovanović, Darka
AU  - Kostić, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4465
AB  - We provide one excel (with four sheets) and 4 csv files. The data in the excel is the same as the one provided in the 4 csv files. 

Data file named 'data plants 1393603.xls' is consisted of 4 sheets containing raw data on all analyzed traits in the manuscript. 

The first sheet named 'antifeeding' contains data on absolute and relative deterrence coefficients of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The second sheet named 'mortality' contains data on the percentage of mortality of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The third sheet named 'molting' contains data on the percentage of 2nd to 3rd instar molting and percentage of molting reduction of gypsy moth larvae exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The forth sheet named 'growth and nutritional indices' contains data on the initial mass, amount of consumed, assimilated and metabolized food, and indices RGR, RCR, RMR, ECI, AD, ECD, and metabolic cost of gypsy moth larvae (4th instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard.
PB  - Basel: MDPI
T2  - Plants
T1  - Potential of Essential Oils for the Gypsy Moth Control
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4465
ER  - 

@misc{
author = "Kostić, igor and Lazarević, Jelica and Šešlija Jovanović, Darka and Kostić, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "We provide one excel (with four sheets) and 4 csv files. The data in the excel is the same as the one provided in the 4 csv files. 

Data file named 'data plants 1393603.xls' is consisted of 4 sheets containing raw data on all analyzed traits in the manuscript. 

The first sheet named 'antifeeding' contains data on absolute and relative deterrence coefficients of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The second sheet named 'mortality' contains data on the percentage of mortality of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The third sheet named 'molting' contains data on the percentage of 2nd to 3rd instar molting and percentage of molting reduction of gypsy moth larvae exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The forth sheet named 'growth and nutritional indices' contains data on the initial mass, amount of consumed, assimilated and metabolized food, and indices RGR, RCR, RMR, ECI, AD, ECD, and metabolic cost of gypsy moth larvae (4th instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Potential of Essential Oils for the Gypsy Moth Control",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4465"
}

Kostić, i., Lazarević, J., Šešlija Jovanović, D., Kostić, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils for the Gypsy Moth Control. in Plants
Basel: MDPI..
https://hdl.handle.net/21.15107/rcub_ibiss_4465

Kostić I, Lazarević J, Šešlija Jovanović D, Kostić M, Marković T, Milanović S. Potential of Essential Oils for the Gypsy Moth Control. in Plants. 2021;.
https://hdl.handle.net/21.15107/rcub_ibiss_4465 .

Kostić, igor, Lazarević, Jelica, Šešlija Jovanović, Darka, Kostić, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils for the Gypsy Moth Control" in Plants (2021),
https://hdl.handle.net/21.15107/rcub_ibiss_4465 .

TY  - CONF
AU  - Nikolić, Biljana
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Radulović, Milena
AU  - Mitić Ćulafić, Dragana
AU  - Đukanović, Stefana
AU  - Ćirić, Ana
AU  - Tasić, Gvozden
AU  - Bošković, Marko
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4376
AB  - The interest in natural products applications as antimicrobial agents is rapidly growing, mainly due to 
increasing microbial resistance to conventional therapeutics. Essential oils (EOs) seem to be especially 
attractive, since many of them possess high antimicrobial potential and none revealed resistance issue. EOs 
have already found applications in endodontic practice, mainly as sealers, but their antimicrobial potential 
has not been explored in this field yet. Taking this into account, the objective of this study was to investigate 
the antibacterial and antibiofilm efficacy of Cymbopogon martinii EO against bacterial strains relevant for 
endodontic therapy. Chemical composition of EO was determined by GC-MS/FID analysis. Antibacterial 
potential was screened in microdilution assay, detecting minimal inhibitory and bactericidal concentrations 
(MICs and MBCs) and performed on Enterococcus faecalis, Streptococcus mitis and Streptococcus 
sanguinis. Eradicating potential against biofilms preformed of each bacterial species as well as of all three 
bacteria, was determined in vitro in crystal violet (CV) assay. For screening of in situ effect against biofilm 
preformed in root canals of extracted teeth, the EO-based irrigant was formulated. Its antibiofilm potential 
was evaluated by plate counting (PC) assay, performed with single E. faecalis biofilm and multispecies 
biofilm composed of all three strains. Single E. faecalis biofilm was selected since it is the most relevant 
intracanal pathogen, while multispecies biofilm was screened to better mimic clinical conditions.
Chemical analysis revealed that geraniol was the most abundant constituent (83.5%), followed by geranyl 
acetate (8.4%). Determined MIC and MBC values were the lowest for E. faecalis (0.12 and 0.25 mg mL-1
, 
respectively), and the highest for S. sanguinis (2.95 and 5.90 mg mL-1
, respectively). CV assay pointed out 
that EO successfully eradicated in vitro preformed biofilms of E. faecalis and S. mitis (approximately 30% 
reduction), but had no effect on S. sanguinis and multispecies biofilms. EO-based irrigant used for 
determination of in situ antibiofilm potential was pre-screened for its physicochemical characteristics: a 
mean particle diameter and polydispersity index was 101 ± 3 nm and 0.36 ± 0.06, respectively. PC assay 
showed that eradicating potential of applied irrigant was weak against both E. faecalis and multispecies 
intracanal biofilm (reduction of logCFU was 0.76 and 0.52, respectively). However, successive irrigation 
with conventional dental irrigants (sodium hypochlorite and sterile saline), followed by C. martini EO-based 
irrigant, notably increased the biofilm reduction (1.89 and 1.69, respectively).
In conclusion, C. martini EO possesses notable antibacterial potential that could be further investigated for 
different dental applications.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4376
ER  - 

@conference{
author = "Nikolić, Biljana and Marinković, Jelena and Marković, Tatjana and Radulović, Milena and Mitić Ćulafić, Dragana and Đukanović, Stefana and Ćirić, Ana and Tasić, Gvozden and Bošković, Marko and Marković, Dejan",
year = "2021",
abstract = "The interest in natural products applications as antimicrobial agents is rapidly growing, mainly due to 
increasing microbial resistance to conventional therapeutics. Essential oils (EOs) seem to be especially 
attractive, since many of them possess high antimicrobial potential and none revealed resistance issue. EOs 
have already found applications in endodontic practice, mainly as sealers, but their antimicrobial potential 
has not been explored in this field yet. Taking this into account, the objective of this study was to investigate 
the antibacterial and antibiofilm efficacy of Cymbopogon martinii EO against bacterial strains relevant for 
endodontic therapy. Chemical composition of EO was determined by GC-MS/FID analysis. Antibacterial 
potential was screened in microdilution assay, detecting minimal inhibitory and bactericidal concentrations 
(MICs and MBCs) and performed on Enterococcus faecalis, Streptococcus mitis and Streptococcus 
sanguinis. Eradicating potential against biofilms preformed of each bacterial species as well as of all three 
bacteria, was determined in vitro in crystal violet (CV) assay. For screening of in situ effect against biofilm 
preformed in root canals of extracted teeth, the EO-based irrigant was formulated. Its antibiofilm potential 
was evaluated by plate counting (PC) assay, performed with single E. faecalis biofilm and multispecies 
biofilm composed of all three strains. Single E. faecalis biofilm was selected since it is the most relevant 
intracanal pathogen, while multispecies biofilm was screened to better mimic clinical conditions.
Chemical analysis revealed that geraniol was the most abundant constituent (83.5%), followed by geranyl 
acetate (8.4%). Determined MIC and MBC values were the lowest for E. faecalis (0.12 and 0.25 mg mL-1
, 
respectively), and the highest for S. sanguinis (2.95 and 5.90 mg mL-1
, respectively). CV assay pointed out 
that EO successfully eradicated in vitro preformed biofilms of E. faecalis and S. mitis (approximately 30% 
reduction), but had no effect on S. sanguinis and multispecies biofilms. EO-based irrigant used for 
determination of in situ antibiofilm potential was pre-screened for its physicochemical characteristics: a 
mean particle diameter and polydispersity index was 101 ± 3 nm and 0.36 ± 0.06, respectively. PC assay 
showed that eradicating potential of applied irrigant was weak against both E. faecalis and multispecies 
intracanal biofilm (reduction of logCFU was 0.76 and 0.52, respectively). However, successive irrigation 
with conventional dental irrigants (sodium hypochlorite and sterile saline), followed by C. martini EO-based 
irrigant, notably increased the biofilm reduction (1.89 and 1.69, respectively).
In conclusion, C. martini EO possesses notable antibacterial potential that could be further investigated for 
different dental applications.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4376"
}

Nikolić, B., Marinković, J., Marković, T., Radulović, M., Mitić Ćulafić, D., Đukanović, S., Ćirić, A., Tasić, G., Bošković, M.,& Marković, D.. (2021). Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_4376

Nikolić B, Marinković J, Marković T, Radulović M, Mitić Ćulafić D, Đukanović S, Ćirić A, Tasić G, Bošković M, Marković D. Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_4376 .

Nikolić, Biljana, Marinković, Jelena, Marković, Tatjana, Radulović, Milena, Mitić Ćulafić, Dragana, Đukanović, Stefana, Ćirić, Ana, Tasić, Gvozden, Bošković, Marko, Marković, Dejan, "Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):109,
https://hdl.handle.net/21.15107/rcub_ibiss_4376 .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Mitić Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Đukanović, Stefana
AU  - Marković, Tatjana
AU  - Tasić, Gvozden
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32707220
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3831
AB  - OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.
PB  - Elsevier Ltd
T2  - Archives of Oral Biology
T1  - Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.
VL  - 117
DO  - 10.1016/j.archoralbio.2020.104842
SP  - 104842
ER  - 

@article{
author = "Marinković, Jelena and Mitić Ćulafić, Dragana and Nikolić, Biljana and Đukanović, Stefana and Marković, Tatjana and Tasić, Gvozden and Ćirić, Ana and Marković, Dejan",
year = "2020",
abstract = "OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.",
publisher = "Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.",
volume = "117",
doi = "10.1016/j.archoralbio.2020.104842",
pages = "104842"
}

Marinković, J., Mitić Ćulafić, D., Nikolić, B., Đukanović, S., Marković, T., Tasić, G., Ćirić, A.,& Marković, D.. (2020). Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology
Elsevier Ltd., 117, 104842.
https://doi.org/10.1016/j.archoralbio.2020.104842

Marinković J, Mitić Ćulafić D, Nikolić B, Đukanović S, Marković T, Tasić G, Ćirić A, Marković D. Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology. 2020;117:104842.
doi:10.1016/j.archoralbio.2020.104842 .

Marinković, Jelena, Mitić Ćulafić, Dragana, Nikolić, Biljana, Đukanović, Stefana, Marković, Tatjana, Tasić, Gvozden, Ćirić, Ana, Marković, Dejan, "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals." in Archives of Oral Biology, 117 (2020):104842,
https://doi.org/10.1016/j.archoralbio.2020.104842 . .

TY  - CHAP
AU  - Marković, Tatjana
AU  - Nikolić, Miloš
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Emerald, Mila
AU  - Radanović, Dragoja
AU  - Zheljazkov, Valtcho
AU  - Soković, Marina
AU  - Valtcho D., Jeliazkov (Zheljazkov)
AU  - Charles L., Cantrell
PY  - 2016
UR  - http://pubs.acs.org/doi/abs/10.1021/bk-2016-1218.ch015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2501
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84987638092&origin=resultslist&sort=plf-f&src=s&st1=%22Essential+Oils+for+the+Prevention+and+Treatment+of+Human+Opportunistic+Fungal+Diseases%22&st2=&sid=BC239BCE0A831C04144486E63BFE49F7.wsnAw8kcdt7IPYLO0V48gA%3a1610&sot=b&sdt=b&sl=103&s=TITLE-ABS-KEY%28%22Essential+Oils+for+the+Prevention+and+Treatment+of+Human+Opportunistic+Fungal+Diseases%22%29&relpos=0&citeCnt=0&searchTerm=
AB  - Human infectious diseases have significantly increased during the past decade, especially among immunocompromised patients. As high as 10% of hospital acquired systemic infections are caused by fungi. Among animal and human pathogens, the dermatomycetes group is the main cause of dermatomycoses, which are chronic but not life threatening infections that bring about considerable morbidity. Under certain circumstances Candida spp. commensals (ie, C. albicans and some non-albicans species) can become pathogenic, which causes amucous membrane infection to turn into a life-threatening systemic disease, particularly in patients with a weakened immune system. In addition to increased resistance of human pathogens to current commercial drugs, conventional treatments have many adverse side effects, thus drugs may become insufficient for treatment and this presents a serious medical problem. Hence, the development of more effective and less toxic antifungal agents, including natural products, is vitally important. Due to their biologically active secondary metabolites, many plants have been traditionally used in ethnomedicine for their therapeutic antifungal potential, with some recent experimental compounds proven to be promising. Essential oils and/or their individual constituents play an important role as potential therapeutic agents. The main advantage is their lipophilic nature, which allows the compounds to easily penetrate the plasma membrane, in addition to the ability to cure opportunistic fungal diseases without harmful effects on human and animal tissues. With growing interest in the use of essential oils in the pharmaceutical industry, systematic examination of their preventive and therapeutic properties has become increasingly important. This paper reviews the antifungal efficacy of essential oils belonging to various plant families for the prevention and treatment of common opportunistic diseases in humans (i.e., dermatomycosis and candidosis), specifically those infections caused by fungi with primary entry routes through skin and mucosa.
PB  - American Chemical Society, Washington, D.C.
T2  - Medicinal and Aromatic Crops: Production, Phytochemistry, and Utilization
T1  - Essential Oils for the Prevention and Treatment of Human Opportunistic Fungal Diseases
DO  - 10.1021/bk-2016-1218.ch015
SP  - 247
EP  - 277
ER  - 

@inbook{
author = "Marković, Tatjana and Nikolić, Miloš and Glamočlija, Jasmina and Ćirić, Ana and Emerald, Mila and Radanović, Dragoja and Zheljazkov, Valtcho and Soković, Marina and Valtcho D., Jeliazkov (Zheljazkov) and Charles L., Cantrell",
year = "2016",
abstract = "Human infectious diseases have significantly increased during the past decade, especially among immunocompromised patients. As high as 10% of hospital acquired systemic infections are caused by fungi. Among animal and human pathogens, the dermatomycetes group is the main cause of dermatomycoses, which are chronic but not life threatening infections that bring about considerable morbidity. Under certain circumstances Candida spp. commensals (ie, C. albicans and some non-albicans species) can become pathogenic, which causes amucous membrane infection to turn into a life-threatening systemic disease, particularly in patients with a weakened immune system. In addition to increased resistance of human pathogens to current commercial drugs, conventional treatments have many adverse side effects, thus drugs may become insufficient for treatment and this presents a serious medical problem. Hence, the development of more effective and less toxic antifungal agents, including natural products, is vitally important. Due to their biologically active secondary metabolites, many plants have been traditionally used in ethnomedicine for their therapeutic antifungal potential, with some recent experimental compounds proven to be promising. Essential oils and/or their individual constituents play an important role as potential therapeutic agents. The main advantage is their lipophilic nature, which allows the compounds to easily penetrate the plasma membrane, in addition to the ability to cure opportunistic fungal diseases without harmful effects on human and animal tissues. With growing interest in the use of essential oils in the pharmaceutical industry, systematic examination of their preventive and therapeutic properties has become increasingly important. This paper reviews the antifungal efficacy of essential oils belonging to various plant families for the prevention and treatment of common opportunistic diseases in humans (i.e., dermatomycosis and candidosis), specifically those infections caused by fungi with primary entry routes through skin and mucosa.",
publisher = "American Chemical Society, Washington, D.C.",
journal = "Medicinal and Aromatic Crops: Production, Phytochemistry, and Utilization",
booktitle = "Essential Oils for the Prevention and Treatment of Human Opportunistic Fungal Diseases",
doi = "10.1021/bk-2016-1218.ch015",
pages = "247-277"
}

Marković, T., Nikolić, M., Glamočlija, J., Ćirić, A., Emerald, M., Radanović, D., Zheljazkov, V., Soković, M., Valtcho D., J. (.,& Charles L., C.. (2016). Essential Oils for the Prevention and Treatment of Human Opportunistic Fungal Diseases. in Medicinal and Aromatic Crops: Production, Phytochemistry, and Utilization
American Chemical Society, Washington, D.C.., 247-277.
https://doi.org/10.1021/bk-2016-1218.ch015

Marković T, Nikolić M, Glamočlija J, Ćirić A, Emerald M, Radanović D, Zheljazkov V, Soković M, Valtcho D. J(, Charles L. C. Essential Oils for the Prevention and Treatment of Human Opportunistic Fungal Diseases. in Medicinal and Aromatic Crops: Production, Phytochemistry, and Utilization. 2016;:247-277.
doi:10.1021/bk-2016-1218.ch015 .

Marković, Tatjana, Nikolić, Miloš, Glamočlija, Jasmina, Ćirić, Ana, Emerald, Mila, Radanović, Dragoja, Zheljazkov, Valtcho, Soković, Marina, Valtcho D., Jeliazkov (Zheljazkov), Charles L., Cantrell, "Essential Oils for the Prevention and Treatment of Human Opportunistic Fungal Diseases" in Medicinal and Aromatic Crops: Production, Phytochemistry, and Utilization (2016):247-277,
https://doi.org/10.1021/bk-2016-1218.ch015 . .

TY  - JOUR
AU  - Nikolić, Milos MN
AU  - Marković, Tatjana
AU  - Mojović, Milos D
AU  - Pejin, Boris
AU  - Savić, Aleksandar G
AU  - Perić, Tamara O
AU  - Marković, Dejan Lj
AU  - Stević, Tatjana R
AU  - Soković, Marina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/982
AB  - The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Industrial Crops and Products
T1  - Chemical composition and biological activity of Gaultheria procumbens L. essential oil
IS  - null
VL  - 49
SP  - 344
EP  - 567
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_982
ER  - 

@article{
author = "Nikolić, Milos MN and Marković, Tatjana and Mojović, Milos D and Pejin, Boris and Savić, Aleksandar G and Perić, Tamara O and Marković, Dejan Lj and Stević, Tatjana R and Soković, Marina",
year = "2013",
abstract = "The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Industrial Crops and Products",
title = "Chemical composition and biological activity of Gaultheria procumbens L. essential oil",
number = "null",
volume = "49",
pages = "344-567",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_982"
}

Nikolić, M. M., Marković, T., Mojović, M. D., Pejin, B., Savić, A. G., Perić, T. O., Marković, D. L., Stević, T. R.,& Soković, M.. (2013). Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops and Products, 49(null), 344-567.
https://hdl.handle.net/21.15107/rcub_ibiss_982

Nikolić MM, Marković T, Mojović MD, Pejin B, Savić AG, Perić TO, Marković DL, Stević TR, Soković M. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops and Products. 2013;49(null):344-567.
https://hdl.handle.net/21.15107/rcub_ibiss_982 .

Nikolić, Milos MN, Marković, Tatjana, Mojović, Milos D, Pejin, Boris, Savić, Aleksandar G, Perić, Tamara O, Marković, Dejan Lj, Stević, Tatjana R, Soković, Marina, "Chemical composition and biological activity of Gaultheria procumbens L. essential oil" in Industrial Crops and Products, 49, no. null (2013):344-567,
https://hdl.handle.net/21.15107/rcub_ibiss_982 .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Perić, Tamara
AU  - Soković, Marina
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/409
AB  - The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils of peppermint Mentha piperita L. GC / MS techniques confirmed the presence of the 48 components of which menthol (35.57 %) and menthone (22.50 %) were the most abundant. Antimicrobial activity, ie the minimum inhibitory and minimum bactericidal / fungicidal (MIC, MBC/MFC) concentrations were obtained using the mikordilution method against pathogenic microorganism isolated from the oral cavity (8 bacteria and 58 Candida sp.) and referent strains. The essential oil of peppermint showed good antimicrobial activity with MIC from 0.63 to 5 mg / ml and 0.13 to 0.5 for bacteria and fungi and MBC 1.25 to 10 mg / ml , ie. MFC 0.5-1 mg / ml. Compared to Hexoral®, peppermint essential oil has a very strong antimicrobial potential. The results of these tests give a new value, which will enrich the knowledge of peppermint oil od presented chemical composition, opens the door for further research towards its use in dentistry and as well in prevention and treatment of various diseases caused by tested pathogens.
AB  - Cilj ovog rada je bio da se ispita hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane Mentha piperita L. GC/MS analizom utvrđeno je prisustvo ukupno 48 komponenata od koji su mentol (35.57 %) i menton (22.50 %) bile najzastupljenije. Antimikobna aktivnost tj minimalne inhibitorne i minimalne baktericidne/fungicidne (MIC, MBC/MFC) koncentracije dobijene su mikordilucionom metodom na patogene mikoorganizme izolovane iz usne duplje (8 bakterija i 58 Candida spp.) i referentne sojeve. Etarsko ulje pitome nane je pokazalo dobru antimikrobnu aktivnost, sa MIC 0.63-5 mg/ml i 0.13-0.5 za bakterije i gljive i MBC 1.25-10.0 mg/ml tj. MFC 0.5-1.0 mg/ml. U poređenju sa Hexoralom®, etarsko ulje pitome nane je posedovalo veoma jak antimikrobni potencijal. Rezultati ovih ispitivanja daju novu vrednost, koja će obogatiti saznanja o etarskom ulju pitome nane poznatog hemijskog sastava i otvarati vrata za dalja istraživanja ka korišćenju njenog etarskog ulja kao prirodnog terapeutskog preparata u stomatologiji, kako u preventivi tako i u tretmanima raznih oboljenja biljaka, životinja i ljudi.
T2  - Lekovite sirovine
T1  - Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.)
T1  - Chemical composition and antimicrobial activity of peppermint essential oil (Mentha piperita L.)
IS  - 33
VL  - null
SP  - 63
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_409
ER  - 

@article{
author = "Nikolić, Miloš and Glamočlija, Jasmina and Ćirić, Ana and Marković, Tatjana and Marković, Dejan and Perić, Tamara and Soković, Marina",
year = "2013, 2013",
abstract = "The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils of peppermint Mentha piperita L. GC / MS techniques confirmed the presence of the 48 components of which menthol (35.57 %) and menthone (22.50 %) were the most abundant. Antimicrobial activity, ie the minimum inhibitory and minimum bactericidal / fungicidal (MIC, MBC/MFC) concentrations were obtained using the mikordilution method against pathogenic microorganism isolated from the oral cavity (8 bacteria and 58 Candida sp.) and referent strains. The essential oil of peppermint showed good antimicrobial activity with MIC from 0.63 to 5 mg / ml and 0.13 to 0.5 for bacteria and fungi and MBC 1.25 to 10 mg / ml , ie. MFC 0.5-1 mg / ml. Compared to Hexoral®, peppermint essential oil has a very strong antimicrobial potential. The results of these tests give a new value, which will enrich the knowledge of peppermint oil od presented chemical composition, opens the door for further research towards its use in dentistry and as well in prevention and treatment of various diseases caused by tested pathogens., Cilj ovog rada je bio da se ispita hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane Mentha piperita L. GC/MS analizom utvrđeno je prisustvo ukupno 48 komponenata od koji su mentol (35.57 %) i menton (22.50 %) bile najzastupljenije. Antimikobna aktivnost tj minimalne inhibitorne i minimalne baktericidne/fungicidne (MIC, MBC/MFC) koncentracije dobijene su mikordilucionom metodom na patogene mikoorganizme izolovane iz usne duplje (8 bakterija i 58 Candida spp.) i referentne sojeve. Etarsko ulje pitome nane je pokazalo dobru antimikrobnu aktivnost, sa MIC 0.63-5 mg/ml i 0.13-0.5 za bakterije i gljive i MBC 1.25-10.0 mg/ml tj. MFC 0.5-1.0 mg/ml. U poređenju sa Hexoralom®, etarsko ulje pitome nane je posedovalo veoma jak antimikrobni potencijal. Rezultati ovih ispitivanja daju novu vrednost, koja će obogatiti saznanja o etarskom ulju pitome nane poznatog hemijskog sastava i otvarati vrata za dalja istraživanja ka korišćenju njenog etarskog ulja kao prirodnog terapeutskog preparata u stomatologiji, kako u preventivi tako i u tretmanima raznih oboljenja biljaka, životinja i ljudi.",
journal = "Lekovite sirovine",
title = "Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.), Chemical composition and antimicrobial activity of peppermint essential oil (Mentha piperita L.)",
number = "33",
volume = "null",
pages = "63-72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_409"
}

Nikolić, M., Glamočlija, J., Ćirić, A., Marković, T., Marković, D., Perić, T.,& Soković, M.. (2013). Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.). in Lekovite sirovine, null(33), 63-72.
https://hdl.handle.net/21.15107/rcub_ibiss_409

Nikolić M, Glamočlija J, Ćirić A, Marković T, Marković D, Perić T, Soković M. Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.). in Lekovite sirovine. 2013;null(33):63-72.
https://hdl.handle.net/21.15107/rcub_ibiss_409 .

Nikolić, Miloš, Glamočlija, Jasmina, Ćirić, Ana, Marković, Tatjana, Marković, Dejan, Perić, Tamara, Soković, Marina, "Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.)" in Lekovite sirovine, null, no. 33 (2013):63-72,
https://hdl.handle.net/21.15107/rcub_ibiss_409 .

TY  - JOUR
AU  - Marković, Tatjana
AU  - Chatzopoulou, Paschalina
AU  - Petrović, Jovana
AU  - Nikolić, M.
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/279
AB  - This work covers the chemical composition and antimicrobial activities of the essential oils isolated from Satureja thymbra and Thymbra spicata analyzed using GC/MS. The main components of S. thymbra oil were thymol (33.8 %), γ-terpinene (30.8 %) and p-cymene (11.8 %). The main components in T. spicata oil were carvacrol (74.5 %) and γ-terpinene (11.2 %). The oils and their main components, thymol and carvacrol, were assayed by applying the microdilution method for antibacterial and antifungal activity against food poisoning, plant, animal and human pathogenic microorganisms. The oil of T. spicata and carvacrol showed the highest antimicrobial activity.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components
IS  - 2
VL  - 63
DO  - 10.2298/ABS1102457M
SP  - 457
EP  - 464
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_279
ER  - 

@article{
author = "Marković, Tatjana and Chatzopoulou, Paschalina and Petrović, Jovana and Nikolić, M. and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2011, 2011",
abstract = "This work covers the chemical composition and antimicrobial activities of the essential oils isolated from Satureja thymbra and Thymbra spicata analyzed using GC/MS. The main components of S. thymbra oil were thymol (33.8 %), γ-terpinene (30.8 %) and p-cymene (11.8 %). The main components in T. spicata oil were carvacrol (74.5 %) and γ-terpinene (11.2 %). The oils and their main components, thymol and carvacrol, were assayed by applying the microdilution method for antibacterial and antifungal activity against food poisoning, plant, animal and human pathogenic microorganisms. The oil of T. spicata and carvacrol showed the highest antimicrobial activity.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components",
number = "2",
volume = "63",
doi = "10.2298/ABS1102457M",
pages = "457-464",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_279"
}

Marković, T., Chatzopoulou, P., Petrović, J., Nikolić, M., Glamočlija, J., Ćirić, A.,& Soković, M.. (2011). Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 63(2), 457-464.
https://doi.org/10.2298/ABS1102457M
https://hdl.handle.net/21.15107/rcub_ibiss_279

Marković T, Chatzopoulou P, Petrović J, Nikolić M, Glamočlija J, Ćirić A, Soković M. Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components. in Archives of Biological Sciences. 2011;63(2):457-464.
doi:10.2298/ABS1102457M
https://hdl.handle.net/21.15107/rcub_ibiss_279 .

Marković, Tatjana, Chatzopoulou, Paschalina, Petrović, Jovana, Nikolić, M., Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components" in Archives of Biological Sciences, 63, no. 2 (2011):457-464,
https://doi.org/10.2298/ABS1102457M .,
https://hdl.handle.net/21.15107/rcub_ibiss_279 .