TY  - JOUR
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
AU  - Jerinić-Prodanović, Dušanka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6665
AB  - Abstract
Background Carrot is an important vegetable crop grown worldwide. The major economic problem in carrot cultivation is yellow disease caused by Bactericera trigonica, which induces biotic stress and has the greatest impact on crop productivity. Comprehensive studies on the mechanism of carrot defense response to biotic stress caused by B. trigonica infestation have yet to be conducted.
Methods The changes in photosynthetic pigments, proline, TPC, H2O2 and MDA content, DPPH radical scavenging ability, and antioxidant enzyme activity of SOD, CAT, and POX in carrot leaves in response to insect sex (female and male), rapid response (during the frst six hours), and long-term response to B. trigonica infestation were evaluated.
Results The results of our study strongly suggest that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and oxidative status of carrot leaves. Photosynthetic pigment content, TPC, and DPPH and CAT activities were signifcantly reduced in carrot leaves in response to insect infestation. On the other hand, proline, H2O2 content, and the activity of the antioxidant enzymes superoxide dismutase and peroxidase were increased in carrot leaves after B. trigonica infestation. The results indicate that B. trigonica attenuates and delays the oxidative stress responses of carrot, allowing long-term feeding without visible changes in the plant. Carrot responded to longterm B. trigonica infestation with an increase in SOD and POX activity, suggesting that these enzymes may play a key
role in plant defense mechanisms.
Conclusions This is the frst comprehensive study strongly suggesting that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and an attenuated ROS defense response in carrot leaves that enables long-term insect feeding. The information provides new insights into the mechanisms of carrot protection against B. trigonica infestation.
PB  - Springer Nature
T2  - BMC Plant Biology
T1  - Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation
IS  - 1
VL  - 24
DO  - 10.1186/s12870-024-04946-4
SP  - 243
ER  - 

@article{
author = "Đurić, Marija and Jevremović, Slađana and Trifunović-Momčilov, Milana and Milošević, Snežana and Subotić, Angelina and Jerinić-Prodanović, Dušanka",
year = "2024",
abstract = "Abstract
Background Carrot is an important vegetable crop grown worldwide. The major economic problem in carrot cultivation is yellow disease caused by Bactericera trigonica, which induces biotic stress and has the greatest impact on crop productivity. Comprehensive studies on the mechanism of carrot defense response to biotic stress caused by B. trigonica infestation have yet to be conducted.
Methods The changes in photosynthetic pigments, proline, TPC, H2O2 and MDA content, DPPH radical scavenging ability, and antioxidant enzyme activity of SOD, CAT, and POX in carrot leaves in response to insect sex (female and male), rapid response (during the frst six hours), and long-term response to B. trigonica infestation were evaluated.
Results The results of our study strongly suggest that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and oxidative status of carrot leaves. Photosynthetic pigment content, TPC, and DPPH and CAT activities were signifcantly reduced in carrot leaves in response to insect infestation. On the other hand, proline, H2O2 content, and the activity of the antioxidant enzymes superoxide dismutase and peroxidase were increased in carrot leaves after B. trigonica infestation. The results indicate that B. trigonica attenuates and delays the oxidative stress responses of carrot, allowing long-term feeding without visible changes in the plant. Carrot responded to longterm B. trigonica infestation with an increase in SOD and POX activity, suggesting that these enzymes may play a key
role in plant defense mechanisms.
Conclusions This is the frst comprehensive study strongly suggesting that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and an attenuated ROS defense response in carrot leaves that enables long-term insect feeding. The information provides new insights into the mechanisms of carrot protection against B. trigonica infestation.",
publisher = "Springer Nature",
journal = "BMC Plant Biology",
title = "Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation",
number = "1",
volume = "24",
doi = "10.1186/s12870-024-04946-4",
pages = "243"
}

Đurić, M., Jevremović, S., Trifunović-Momčilov, M., Milošević, S., Subotić, A.,& Jerinić-Prodanović, D.. (2024). Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation. in BMC Plant Biology
Springer Nature., 24(1), 243.
https://doi.org/10.1186/s12870-024-04946-4

Đurić M, Jevremović S, Trifunović-Momčilov M, Milošević S, Subotić A, Jerinić-Prodanović D. Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation. in BMC Plant Biology. 2024;24(1):243.
doi:10.1186/s12870-024-04946-4 .

Đurić, Marija, Jevremović, Slađana, Trifunović-Momčilov, Milana, Milošević, Snežana, Subotić, Angelina, Jerinić-Prodanović, Dušanka, "Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation" in BMC Plant Biology, 24, no. 1 (2024):243,
https://doi.org/10.1186/s12870-024-04946-4 . .

TY  - CHAP
AU  - Đurić, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6485
AB  - Povećanje prosečnih godišnjih temperatura iz godine u godinu za posledicu ostavlja sve izraženije sušne
sezone. Suša nepovoljno utiče na rastenje i razviće biljaka, uzrokujući velike ekonomske gubitke na globalnom
nivou. Efekti suše ispoljavaju se kako na morfološkom, tako i na fiziološkom, biohemijskom i molekularnom
nivou organizacije biljaka. Izlaganje biljaka suši narušava normalan transport vode kroz ćelije
što se odražava i na zastupljenost i funkciju kanala za vodu – akvaporina, na membranama. Akvaporini su
transmembranski proteini koji formiraju kanale za prolazak vode i drugih molekula kroz ćelijske membrane.
Ispoljavaju veoma značajnu ulogu u prilagođavanju protoka vode kroz ćelije shodno fiziološkom stanju.
S obzirom na to, poslednjih godina velika pažnja posvećena je ulozi akvaporina pri odgovorima biljaka
na dejstvo suše i drugih abiotičkih stresogenih faktora koji utiču na narušavanje vodnog režima biljaka.
Istraživanja su usmerena ka praćenju promena u ekspresiji gena za akvaporine, zastupljenosti akvaporina
na membranama i uticaju na usvajanje, transport i odavanje vode u atmosferu. U ovom radu predstavljene
su analize strukture i regulacije ekspresije gena za akvaporine, kao i naučna istraživanja u proteklih
deset godina o promenama u ekspresiji gena za akvaporine kod biljaka izlaganih suši. Poseban akcenat
stavljen je na rezultate bioinformatičkih analiza akvaporina kod hortikulturne vrste Impatiens walleriana,
i njihovu ekspresiju pri suši i rehidrataciji.
AB  - The increase in average annual temperatures from year to year results in pronounced dry seasons. Drought
adversely affects the plants growth and development, causing large economic losses on a global scale.
The effects of drought are manifested both at the plant morphological, as well at the physiological, bio-
chemical and molecular levels. Exposure of plants to drought disrupts the normal water transport through
the cells, which is reflected in the presence and function of water channels - aquaporins, on the mem-
branes. Aquaporins are transmembrane proteins that form channels for the water, and other molecules
flow through cell membranes. They play a very important role in adjusting the water flow through the
cells according to the physiological state. In view of this, in recent years much attention has been paid to
the role of aquaporins in plant responses to drought and other abiotic stress factors, which affect the dis-
ruption of the water regime of plants. Researches are aimed at monitoring changes in the expression of
genes for aquaporins, the presence of aquaporins on membranes and the influence on the absorption,
transport and release of water into the atmosphere. This paper presents analyzes of the structure and reg-
ulation of aquaporin gene expression, as well as scientific research over the past ten years on changes in
aquaporin gene expression in plants exposed to drought. Special emphasis was placed on the results of
bioinformatic analyzes of aquaporins in the horticultural species Impatiens walleriana, and their expression
during drought and rehydration.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in Molecular Biology
T1  - Struktura, funkcija i regulacija ekspresije gena za akvaporine pri suši kod biljaka
T1  - Structure, function and regulation of aquaporin gene expression during drought in plants
IS  - 3
SP  - 256
EP  - 268
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6485
ER  - 

@inbook{
author = "Đurić, Marija",
year = "2023",
abstract = "Povećanje prosečnih godišnjih temperatura iz godine u godinu za posledicu ostavlja sve izraženije sušne
sezone. Suša nepovoljno utiče na rastenje i razviće biljaka, uzrokujući velike ekonomske gubitke na globalnom
nivou. Efekti suše ispoljavaju se kako na morfološkom, tako i na fiziološkom, biohemijskom i molekularnom
nivou organizacije biljaka. Izlaganje biljaka suši narušava normalan transport vode kroz ćelije
što se odražava i na zastupljenost i funkciju kanala za vodu – akvaporina, na membranama. Akvaporini su
transmembranski proteini koji formiraju kanale za prolazak vode i drugih molekula kroz ćelijske membrane.
Ispoljavaju veoma značajnu ulogu u prilagođavanju protoka vode kroz ćelije shodno fiziološkom stanju.
S obzirom na to, poslednjih godina velika pažnja posvećena je ulozi akvaporina pri odgovorima biljaka
na dejstvo suše i drugih abiotičkih stresogenih faktora koji utiču na narušavanje vodnog režima biljaka.
Istraživanja su usmerena ka praćenju promena u ekspresiji gena za akvaporine, zastupljenosti akvaporina
na membranama i uticaju na usvajanje, transport i odavanje vode u atmosferu. U ovom radu predstavljene
su analize strukture i regulacije ekspresije gena za akvaporine, kao i naučna istraživanja u proteklih
deset godina o promenama u ekspresiji gena za akvaporine kod biljaka izlaganih suši. Poseban akcenat
stavljen je na rezultate bioinformatičkih analiza akvaporina kod hortikulturne vrste Impatiens walleriana,
i njihovu ekspresiju pri suši i rehidrataciji., The increase in average annual temperatures from year to year results in pronounced dry seasons. Drought
adversely affects the plants growth and development, causing large economic losses on a global scale.
The effects of drought are manifested both at the plant morphological, as well at the physiological, bio-
chemical and molecular levels. Exposure of plants to drought disrupts the normal water transport through
the cells, which is reflected in the presence and function of water channels - aquaporins, on the mem-
branes. Aquaporins are transmembrane proteins that form channels for the water, and other molecules
flow through cell membranes. They play a very important role in adjusting the water flow through the
cells according to the physiological state. In view of this, in recent years much attention has been paid to
the role of aquaporins in plant responses to drought and other abiotic stress factors, which affect the dis-
ruption of the water regime of plants. Researches are aimed at monitoring changes in the expression of
genes for aquaporins, the presence of aquaporins on membranes and the influence on the absorption,
transport and release of water into the atmosphere. This paper presents analyzes of the structure and reg-
ulation of aquaporin gene expression, as well as scientific research over the past ten years on changes in
aquaporin gene expression in plants exposed to drought. Special emphasis was placed on the results of
bioinformatic analyzes of aquaporins in the horticultural species Impatiens walleriana, and their expression
during drought and rehydration.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in Molecular Biology",
booktitle = "Struktura, funkcija i regulacija ekspresije gena za akvaporine pri suši kod biljaka, Structure, function and regulation of aquaporin gene expression during drought in plants",
number = "3",
pages = "256-268",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6485"
}

Đurić, M.. (2023). Struktura, funkcija i regulacija ekspresije gena za akvaporine pri suši kod biljaka. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(3), 256-268.
https://hdl.handle.net/21.15107/rcub_ibiss_6485

Đurić M. Struktura, funkcija i regulacija ekspresije gena za akvaporine pri suši kod biljaka. in Trends in Molecular Biology. 2023;(3):256-268.
https://hdl.handle.net/21.15107/rcub_ibiss_6485 .

Đurić, Marija, "Struktura, funkcija i regulacija ekspresije gena za akvaporine pri suši kod biljaka" in Trends in Molecular Biology, no. 3 (2023):256-268,
https://hdl.handle.net/21.15107/rcub_ibiss_6485 .

TY  - JOUR
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Radulović, Olga
AU  - Subotić, Angelina
AU  - Uzelac, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6135
AB  - Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.
PB  - Basel: MDPI
T2  - Agriculture
T1  - Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?
IS  - 10
VL  - 13
DO  - 10.3390/agriculture13101903
SP  - 1903
ER  - 

@article{
author = "Đurić, Marija and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Radulović, Olga and Subotić, Angelina and Uzelac, Branka",
year = "2023",
abstract = "Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?",
number = "10",
volume = "13",
doi = "10.3390/agriculture13101903",
pages = "1903"
}

Đurić, M., Trifunović-Momčilov, M., Milošević, S., Marković, M., Radulović, O., Subotić, A.,& Uzelac, B.. (2023). Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture
Basel: MDPI., 13(10), 1903.
https://doi.org/10.3390/agriculture13101903

Đurić M, Trifunović-Momčilov M, Milošević S, Marković M, Radulović O, Subotić A, Uzelac B. Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture. 2023;13(10):1903.
doi:10.3390/agriculture13101903 .

Đurić, Marija, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Radulović, Olga, Subotić, Angelina, Uzelac, Branka, "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?" in Agriculture, 13, no. 10 (2023):1903,
https://doi.org/10.3390/agriculture13101903 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5827
AB  - In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.
PB  - Taylor & Francis Group
T2  - Plant Signaling & Behavior
T1  - Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress
IS  - 1
VL  - 18
DO  - 10.1080/15592324.2023.2219936
SP  - e2219936
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.",
publisher = "Taylor & Francis Group",
journal = "Plant Signaling & Behavior",
title = "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress",
number = "1",
volume = "18",
doi = "10.1080/15592324.2023.2219936",
pages = "e2219936"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior
Taylor & Francis Group., 18(1), e2219936.
https://doi.org/10.1080/15592324.2023.2219936

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior. 2023;18(1):e2219936.
doi:10.1080/15592324.2023.2219936 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress" in Plant Signaling & Behavior, 18, no. 1 (2023):e2219936,
https://doi.org/10.1080/15592324.2023.2219936 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5762
AB  - Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general.
PB  - Basel: MDPI
T2  - Genes
T1  - Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application
IS  - 5
VL  - 14
DO  - 10.3390/genes14051072
SP  - 1072
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general.",
publisher = "Basel: MDPI",
journal = "Genes",
title = "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application",
number = "5",
volume = "14",
doi = "10.3390/genes14051072",
pages = "1072"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes
Basel: MDPI., 14(5), 1072.
https://doi.org/10.3390/genes14051072

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes. 2023;14(5):1072.
doi:10.3390/genes14051072 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application" in Genes, 14, no. 5 (2023):1072,
https://doi.org/10.3390/genes14051072 . .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5763
AB  - This research evaluated the effects of foliarly applied elicitor methyl jasmonate (MeJA) on the potential improvement of drought tolerance in Impatiens walleriana. Growth and leaf physiology parameters (Stomatal conductance, Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index - NBI), as well the expression of four aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1), were evaluated. These parameters could serve as indicators of drought tolerance in I. walleriana, a worldwide popular horticultural plant, very sensitive to drought. The experiment included four treatments: control plants, drought-stressed plants foliarly sprayed with distilled water, drought-stressed plants foliarly sprayed with 5 μM MeJA, and drought-stressed plants foliarly sprayed with 50 μM MeJA. Foliar spraying was performed seven days before drought induction, and on the day of drought stress induction. The stressed plant groups were non-irrigated to reach 15 and 5% soil water content (SWC), while control plants were well-watered (35-37% SWC) during the entire experiment. Drought reduced fresh and dry shoot weight, as well total leaf area but dry matter content was not affected. Foliarly applied MeJA improveda growth parameters, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC in plants foliarly sprayed with MeJA regardless of the used concentration. Similarly, the flavonoid index was reduced at 15 and 5% SWC when 50 μM MeJA was foliarly applied, while there were no observed changes in the anthocyanin index in any treatment. The foliar application of 50 μM MeJA increased I. walleriana chlorophyll index and NBI at 5% SWC, indicating an elicitor contribution to plant drought tolerance at physiological level. Among the four analyzed aquaporin genes, expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants foliarly pre-treated with 50 μM MeJA, indicating an improvement of water flow through cells to maintain homeostasis.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina
T1  - Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5763
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "This research evaluated the effects of foliarly applied elicitor methyl jasmonate (MeJA) on the potential improvement of drought tolerance in Impatiens walleriana. Growth and leaf physiology parameters (Stomatal conductance, Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index - NBI), as well the expression of four aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1), were evaluated. These parameters could serve as indicators of drought tolerance in I. walleriana, a worldwide popular horticultural plant, very sensitive to drought. The experiment included four treatments: control plants, drought-stressed plants foliarly sprayed with distilled water, drought-stressed plants foliarly sprayed with 5 μM MeJA, and drought-stressed plants foliarly sprayed with 50 μM MeJA. Foliar spraying was performed seven days before drought induction, and on the day of drought stress induction. The stressed plant groups were non-irrigated to reach 15 and 5% soil water content (SWC), while control plants were well-watered (35-37% SWC) during the entire experiment. Drought reduced fresh and dry shoot weight, as well total leaf area but dry matter content was not affected. Foliarly applied MeJA improveda growth parameters, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC in plants foliarly sprayed with MeJA regardless of the used concentration. Similarly, the flavonoid index was reduced at 15 and 5% SWC when 50 μM MeJA was foliarly applied, while there were no observed changes in the anthocyanin index in any treatment. The foliar application of 50 μM MeJA increased I. walleriana chlorophyll index and NBI at 5% SWC, indicating an elicitor contribution to plant drought tolerance at physiological level. Among the four analyzed aquaporin genes, expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants foliarly pre-treated with 50 μM MeJA, indicating an improvement of water flow through cells to maintain homeostasis.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina",
title = "Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5763"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_5763

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina. 2023;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_5763 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana" in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina (2023):141,
https://hdl.handle.net/21.15107/rcub_ibiss_5763 .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5335
AB  - Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.
PB  - Basel: MDPI
T2  - Life
T1  - Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro
IS  - 1
VL  - 13
DO  - 10.3390/life13010154
SP  - 154
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2023",
abstract = "Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro",
number = "1",
volume = "13",
doi = "10.3390/life13010154",
pages = "154"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2023). Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life
Basel: MDPI., 13(1), 154.
https://doi.org/10.3390/life13010154

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life. 2023;13(1):154.
doi:10.3390/life13010154 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro" in Life, 13, no. 1 (2023):154,
https://doi.org/10.3390/life13010154 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5307
AB  - This study was performed to determine the response of Impatiens walleriana, an important horticultural plant, to exogenous
application of methyl jasmonate (MeJA) during in vitro PEG8000−induced water defcit. Shoots were pretreated with graded
MeJA concentrations (0, 5, 50, and 100 µM) in Murashige and Skoog (MS) medium for 7 days. After pretreatment, shoots
were exposed to diferent treatments for 20 days: MS with polyethylene glycol (PEG8000 −0 or 3%), MS with PEG8000
(3%)+MeJA (5, 50 or 100 µM), and MS with MeJA (5, 50 or 100 µM) only. MeJA pretreatment had positive efects on I.
walleriana growth (fresh weight, plant height, number of leaves per plant and proliferation rate) in non-stressed shoots,
especially after application of the lowest MeJA concentration (5 µM). In the water defcit conditions caused by PEG8000, pretreatment with 5 µM MeJA increased all analyzed growth parameters, and caused decreased photosynthetic pigment, proline
and total amino acid content, total polyphenol and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity, hydrogen peroxide and
malondialdehyde content. Additionally, changes in the activity of antioxidant enzymes (superoxide dismutase, peroxidase
and catalase) were induced. Pretreatment with higher MeJA concentrations (50 and 100 µM) mainly suppressed the I. walleriana shoots growth under water defcit, combination treatments and treatment only with MeJA. These results indicate the
potential of MeJA’s in enhancing I. walleriana ability to withstand the water stress in vitro. I. walleriana improved water
stress resistance by promoting defense-related metabolism, which was mainly induced by pretreatment with the lowest
applied MeJA concentration (5 µM).
PB  - Springer Nature B.V. 2022
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate
DO  - 10.1007/s11240-022-02432-z
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2022",
abstract = "This study was performed to determine the response of Impatiens walleriana, an important horticultural plant, to exogenous
application of methyl jasmonate (MeJA) during in vitro PEG8000−induced water defcit. Shoots were pretreated with graded
MeJA concentrations (0, 5, 50, and 100 µM) in Murashige and Skoog (MS) medium for 7 days. After pretreatment, shoots
were exposed to diferent treatments for 20 days: MS with polyethylene glycol (PEG8000 −0 or 3%), MS with PEG8000
(3%)+MeJA (5, 50 or 100 µM), and MS with MeJA (5, 50 or 100 µM) only. MeJA pretreatment had positive efects on I.
walleriana growth (fresh weight, plant height, number of leaves per plant and proliferation rate) in non-stressed shoots,
especially after application of the lowest MeJA concentration (5 µM). In the water defcit conditions caused by PEG8000, pretreatment with 5 µM MeJA increased all analyzed growth parameters, and caused decreased photosynthetic pigment, proline
and total amino acid content, total polyphenol and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity, hydrogen peroxide and
malondialdehyde content. Additionally, changes in the activity of antioxidant enzymes (superoxide dismutase, peroxidase
and catalase) were induced. Pretreatment with higher MeJA concentrations (50 and 100 µM) mainly suppressed the I. walleriana shoots growth under water defcit, combination treatments and treatment only with MeJA. These results indicate the
potential of MeJA’s in enhancing I. walleriana ability to withstand the water stress in vitro. I. walleriana improved water
stress resistance by promoting defense-related metabolism, which was mainly induced by pretreatment with the lowest
applied MeJA concentration (5 µM).",
publisher = "Springer Nature B.V. 2022",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate",
doi = "10.1007/s11240-022-02432-z"
}

Đurić, M., Subotić, A., Trifunović-Momčilov, M.,& Milošević, S.. (2022). Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate. in Plant Cell, Tissue and Organ Culture (PCTOC)
Springer Nature B.V. 2022..
https://doi.org/10.1007/s11240-022-02432-z

Đurić M, Subotić A, Trifunović-Momčilov M, Milošević S. Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate. in Plant Cell, Tissue and Organ Culture (PCTOC). 2022;.
doi:10.1007/s11240-022-02432-z .

Đurić, Marija, Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, "Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate" in Plant Cell, Tissue and Organ Culture (PCTOC) (2022),
https://doi.org/10.1007/s11240-022-02432-z . .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Trifunović-Momčilov, Milana
AU  - Đurić, Marija
AU  - Koruga, Đuro
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5145
AB  - The rapid production and numerous applications of nanomaterials warrant the necessity
and importance of examining nanoparticles in terms to their environmental and biological effects
and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified
fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and
fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment,
and aquaporin genes expression in cherry tomato plants were studied after during short- and longterm continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic
pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of
cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased
aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstanceexposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance
showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in
fruits and may be considered as a promising nanofertilizer.
PB  - Basel: MDPI
T2  - Plants
T1  - Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex
IS  - 21
VL  - 11
DO  - 10.3390/plants11212810
SP  - 2810
ER  - 

@article{
author = "Subotić, Angelina and Jevremović, Slađana and Milošević, Snežana and Trifunović-Momčilov, Milana and Đurić, Marija and Koruga, Đuro",
year = "2022",
abstract = "The rapid production and numerous applications of nanomaterials warrant the necessity
and importance of examining nanoparticles in terms to their environmental and biological effects
and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified
fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and
fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment,
and aquaporin genes expression in cherry tomato plants were studied after during short- and longterm continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic
pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of
cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased
aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstanceexposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance
showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in
fruits and may be considered as a promising nanofertilizer.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex",
number = "21",
volume = "11",
doi = "10.3390/plants11212810",
pages = "2810"
}

Subotić, A., Jevremović, S., Milošević, S., Trifunović-Momčilov, M., Đurić, M.,& Koruga, Đ.. (2022). Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex. in Plants
Basel: MDPI., 11(21), 2810.
https://doi.org/10.3390/plants11212810

Subotić A, Jevremović S, Milošević S, Trifunović-Momčilov M, Đurić M, Koruga Đ. Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex. in Plants. 2022;11(21):2810.
doi:10.3390/plants11212810 .

Subotić, Angelina, Jevremović, Slađana, Milošević, Snežana, Trifunović-Momčilov, Milana, Đurić, Marija, Koruga, Đuro, "Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex" in Plants, 11, no. 21 (2022):2810,
https://doi.org/10.3390/plants11212810 . .

TY  - THES
AU  - Đurić, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4952
AB  - Drought is one of the abiotic stress facotors and one of the most common and biggest problems today. Drought has a negative impact on the growth and development of numerous plant species, and thus directly or indirectly affects their abundance and distribution, leaving negative consequences on the biodiversity of the entire flora and fauna. This study was carried out to examine the dehydration caused by drought, and rehydration effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana, as well as the effects of the methyl jasmonate (MeJA) elicitation in order to overcome the stress caused by dehydration. I. walleriana (Balsaminaceae) is one of the three Impatiens species (beside I. hawkeri and I. balsamina) which are commercially produced in Serbia for many years. Due to its decorative traits and long flowering period, I. walleriana belongs to the most popular horticultural species all over the world. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in cell turgor and tissue dehydration which is the main problem in commercial production and market placement of these plants. In the first part of the experiment design there were three treatments: control plant groups grown under optimal watering with 35–37% of soil moisture content, stressed plant groups non-irrigated to reach 15 and 5% of soil moisture content and recovered plant groups rehydrated for four days to reach optimal soil moisture content. Dehydration reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Dehydration up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (IwNCED4) and abscisic aldehyde oxidase 2 (IwAAO2) and catabolic gene ABA 8′-hydroxylase 3 (IwABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the dehydration was not accompanied with increased amino acid proline content. Increament in total chlorophyll, carotenoid, polyphenols and flavonols content, as well as malondialdehyde (MDA), hydrogen peroxide (H2O2) and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity, was observed in plants during dehydration. Increased antioxidant enzyme activities (superoxide-dismutase – SOD, peroxidase – POX and catalase – CAT) throughout dehydration were also determined. Rehydration was significant for overcoming dehydration effect on growth parameters, shoot water potential, endogenous proline content and genes expression. Also, analysis of molecular structure and gene expression of aquaporins (AQP) in I. walleriana leaves were estimated. Aquaporins comprise a large group of transmembrane proteins responsible for water transport, which is crucial for plant survival under stress conditions. Despite the vital role of AQPs, nothing is known about this protein family in I. walleriana. In the present doctoral dissertation, attention is given to the molecular characterization of AQPs in I. walleriana and their expression during drought stress and recovery. We identified four I. walleriana AQPs: IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein. Dehydration and rehydration treatment affected the AQPs expression in I. walleriana leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was the most affected of all analyzed I. walleriana AQPs. At 15 and 5% soil moisture and recovery from 15 and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively. Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with moderate expression changes in response to dehydration and rehydration, while IwPIP2;2 expression was significantly increased only in recovered plants. Insight into the molecular structure of I. walleriana AQPs expanded knowledge about plant AQPs, while its expression during dehydration and rehydration contributed to I. walleriana dehydration tolerance mechanisms and re-acclimation. In the second part of the experiment, the effect of MeJA elicitation on overcome dehydration effects on the growth and development of I. walleriana was evaluated. The effect of MeJA elicitation in concentrations of 5 and 50 μM was manifested in both control and plants exposed to dehydration (at 15 and 5% soil moisture content) and rehydration. A positive effect of MeJA was observed on the growth parameters (fresh weight of shoots, total leaf area and dry weight of shoots) in control and plants exposed to different dehydration intensities. Methyl jasmonate induced increament in the expression of genes involved in ABA de novo synthesis during dehydration, as well as the change in the expression of gene involved in ABA catabolism. Also, reducing effect of MeJA elicitation on the stomatal conductivity was noted in control and dehydrated plants. Elicitation increased chlorophyll a/b ratio during lower and higher dehydration intensity, as well as the total chlorophyll and carotenoid content during intensive dehydration. In plant groups treated with MeJA, a decrease in the concentration of MDA and H2O2, as well as the lower content of total polyphenols and flavonols and changed activity of antioxidant enzymes SOD, POX and CAT were observed during dehydration. Elicitation with MeJA differently affected the expression of the AQPs genes IwPIP;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1 during dehydration and rehydration, indicating the importance of the water channels involved in adapting water transport processes according to physiological state of the organism.
PB  - Belgrade: Faculty of Biology, University of Belgrade
T2  - Faculty of Biology, University of Belgrade
T1  - Uticaj dehidratacije i rehidratacije na fiziološki odgovor i ekspresiju gena za akvaporine i metabolizam apscisinske kiseline kod Impatiens walleriana
T1  - Effect of dehydration and rehydration on physiological response and the expression of aquaporin genes and abscisic acid metabolism in Impatiens walleriana
SP  - 1
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4952
ER  - 

@phdthesis{
author = "Đurić, Marija",
year = "2022",
abstract = "Drought is one of the abiotic stress facotors and one of the most common and biggest problems today. Drought has a negative impact on the growth and development of numerous plant species, and thus directly or indirectly affects their abundance and distribution, leaving negative consequences on the biodiversity of the entire flora and fauna. This study was carried out to examine the dehydration caused by drought, and rehydration effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana, as well as the effects of the methyl jasmonate (MeJA) elicitation in order to overcome the stress caused by dehydration. I. walleriana (Balsaminaceae) is one of the three Impatiens species (beside I. hawkeri and I. balsamina) which are commercially produced in Serbia for many years. Due to its decorative traits and long flowering period, I. walleriana belongs to the most popular horticultural species all over the world. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in cell turgor and tissue dehydration which is the main problem in commercial production and market placement of these plants. In the first part of the experiment design there were three treatments: control plant groups grown under optimal watering with 35–37% of soil moisture content, stressed plant groups non-irrigated to reach 15 and 5% of soil moisture content and recovered plant groups rehydrated for four days to reach optimal soil moisture content. Dehydration reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Dehydration up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (IwNCED4) and abscisic aldehyde oxidase 2 (IwAAO2) and catabolic gene ABA 8′-hydroxylase 3 (IwABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the dehydration was not accompanied with increased amino acid proline content. Increament in total chlorophyll, carotenoid, polyphenols and flavonols content, as well as malondialdehyde (MDA), hydrogen peroxide (H2O2) and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity, was observed in plants during dehydration. Increased antioxidant enzyme activities (superoxide-dismutase – SOD, peroxidase – POX and catalase – CAT) throughout dehydration were also determined. Rehydration was significant for overcoming dehydration effect on growth parameters, shoot water potential, endogenous proline content and genes expression. Also, analysis of molecular structure and gene expression of aquaporins (AQP) in I. walleriana leaves were estimated. Aquaporins comprise a large group of transmembrane proteins responsible for water transport, which is crucial for plant survival under stress conditions. Despite the vital role of AQPs, nothing is known about this protein family in I. walleriana. In the present doctoral dissertation, attention is given to the molecular characterization of AQPs in I. walleriana and their expression during drought stress and recovery. We identified four I. walleriana AQPs: IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein. Dehydration and rehydration treatment affected the AQPs expression in I. walleriana leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was the most affected of all analyzed I. walleriana AQPs. At 15 and 5% soil moisture and recovery from 15 and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively. Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with moderate expression changes in response to dehydration and rehydration, while IwPIP2;2 expression was significantly increased only in recovered plants. Insight into the molecular structure of I. walleriana AQPs expanded knowledge about plant AQPs, while its expression during dehydration and rehydration contributed to I. walleriana dehydration tolerance mechanisms and re-acclimation. In the second part of the experiment, the effect of MeJA elicitation on overcome dehydration effects on the growth and development of I. walleriana was evaluated. The effect of MeJA elicitation in concentrations of 5 and 50 μM was manifested in both control and plants exposed to dehydration (at 15 and 5% soil moisture content) and rehydration. A positive effect of MeJA was observed on the growth parameters (fresh weight of shoots, total leaf area and dry weight of shoots) in control and plants exposed to different dehydration intensities. Methyl jasmonate induced increament in the expression of genes involved in ABA de novo synthesis during dehydration, as well as the change in the expression of gene involved in ABA catabolism. Also, reducing effect of MeJA elicitation on the stomatal conductivity was noted in control and dehydrated plants. Elicitation increased chlorophyll a/b ratio during lower and higher dehydration intensity, as well as the total chlorophyll and carotenoid content during intensive dehydration. In plant groups treated with MeJA, a decrease in the concentration of MDA and H2O2, as well as the lower content of total polyphenols and flavonols and changed activity of antioxidant enzymes SOD, POX and CAT were observed during dehydration. Elicitation with MeJA differently affected the expression of the AQPs genes IwPIP;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1 during dehydration and rehydration, indicating the importance of the water channels involved in adapting water transport processes according to physiological state of the organism.",
publisher = "Belgrade: Faculty of Biology, University of Belgrade",
journal = "Faculty of Biology, University of Belgrade",
title = "Uticaj dehidratacije i rehidratacije na fiziološki odgovor i ekspresiju gena za akvaporine i metabolizam apscisinske kiseline kod Impatiens walleriana, Effect of dehydration and rehydration on physiological response and the expression of aquaporin genes and abscisic acid metabolism in Impatiens walleriana",
pages = "1-163",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4952"
}

Đurić, M.. (2022). Uticaj dehidratacije i rehidratacije na fiziološki odgovor i ekspresiju gena za akvaporine i metabolizam apscisinske kiseline kod Impatiens walleriana. in Faculty of Biology, University of Belgrade
Belgrade: Faculty of Biology, University of Belgrade., 1-163.
https://hdl.handle.net/21.15107/rcub_ibiss_4952

Đurić M. Uticaj dehidratacije i rehidratacije na fiziološki odgovor i ekspresiju gena za akvaporine i metabolizam apscisinske kiseline kod Impatiens walleriana. in Faculty of Biology, University of Belgrade. 2022;:1-163.
https://hdl.handle.net/21.15107/rcub_ibiss_4952 .

Đurić, Marija, "Uticaj dehidratacije i rehidratacije na fiziološki odgovor i ekspresiju gena za akvaporine i metabolizam apscisinske kiseline kod Impatiens walleriana" in Faculty of Biology, University of Belgrade (2022):1-163,
https://hdl.handle.net/21.15107/rcub_ibiss_4952 .

TY  - CONF
AU  - Đurić, Marija
AU  - Stamenković, Nikola
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5327
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5327
ER  - 

@conference{
author = "Đurić, Marija and Stamenković, Nikola and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5327"
}

Đurić, M., Stamenković, N., Trifunović-Momčilov, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327

Đurić M, Stamenković N, Trifunović-Momčilov M, Milošević S, Marković M, Giba Z, Subotić A. The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

Đurić, Marija, Stamenković, Nikola, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):60,
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5377
AB  - Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl.
AB  - Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом
T1  - Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5377
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl., Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом, Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5377"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

TY  - CONF
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Marković, Marija
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5398
AB  - Лепи Јова (Impatiens walleriana) је веома популарна хортикултурна врста у Србији 
и свету због својих декоративних особина, дугог периода цветања и широког 
спектра боје цветова. У његовој комерцијалној производњи наводњавање је 
смањено током транспорта и пласмана биљака на тржиште. Суша има неповољан 
утицај на растење и развиће биљака, а у ћелијама доводи до успоравања 
анаболичких и убрзавања катаболичких процеса, уз појачану производњу 
реактивних форми кисеоника (Reactive oxygen species – ROS). Дисбаланс између 
настанка и уклањања ROS има за последицу нарушавање ћелијске хомеостазе и 
оксидативни стрес. Иако ROS имају важну сигналну улогу, њихова висока 
цитотоксичност и реактивност доводе до модификација DNK, RNK, протеина и 
липида мембрана. У овом истраживању испитиван је утицај натријум 
нитропрусида (Sodium nitroprusside – SNP), као донора NO, на физиолошке и 
биохемијске промене in vitro гајеног Лепог Јове у условима суше изазване 
полиетилен гликолом (PEG8000). Изданци (величине 20-25 мм) су гајени на 
хранљивим подлогама са SNP (0, 50, 100 и 250 μM) 20 дана. Након претретмана, 
изданци су пребачени на свеже MS подлоге којима су додати PEG8000 (0 или 3%) 
и/или SNP (0, 50, 100 или 250 μM) и гајени су седам дана. Утицај егзогене примене 
SNP на повећање толеранције Лепог Јове на сушу испољава се у променама 
концентрације H2O2, малондиалдехида и укупних фенолних једињења, као и 
активности актиоксидативних ензима: каталаза, пероксидаза и супероксиддисмутаза у изданцима.
AB  - Lepi Jova (Impatiens walleriana) je veoma popularna hortikulturna vrsta u Srbiji i svetu zbog svojih dekorativnih osobina, dugog perioda cvetanja i širokog spektra boje cvetova. U njegovoj komercijalnoj proizvodnji navodnjavanje je smanjeno tokom transporta i plasmana biljaka na tržište. Suša ima nepovoljan uticaj na rastenje i razviće biljaka, a u ćelijama dovodi do usporavanja anaboličkih i ubrzavanja kataboličkih procesa, uz pojačanu proizvodnju reaktivnih formi kiseonika (Reactive oxygen species – ROS). Disbalans između nastanka i uklanjanja ROS ima za posledicu narušavanje ćelijske homeostaze i oksidativni stres. Iako ROS imaju važnu signalnu ulogu, njihova visoka citotoksičnost i reaktivnost dovode do modifikacija DNK, RNK, proteina i lipida membrana. U ovom istraživanju ispitivan je uticaj natrijum nitroprusida (Sodium nitroprusside – SNP), kao donora NO, na fiziološke i biohemijske promene in vitro gajenog Lepog Jove u uslovima suše izazvane polietilen glikolom (PEG8000). Izdanci (veličine 20-25 mm) su gajeni na hranljivim podlogama sa SNP (0, 50, 100 i 250 μM) 20 dana. Nakon pretretmana, izdanci su prebačeni na sveže MS podloge kojima su dodati PEG8000 (0 ili 3%) i/ili SNP (0, 50, 100 ili 250 μM) i gajeni su sedam dana. Uticaj egzogene primene SNP na povećanje tolerancije Lepog Jove na sušu ispoljava se u promenama koncentracije H2O2, malondialdehida i ukupnih fenolnih jedinjenja, kao i aktivnosti aktioksidativnih enzima: katalaza, peroksidaza i superoksiddismutaza u izdancima.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro
T1  - Uticaj natrijum nitroprusida na fiziološki odgovor Lepog Jove (Impatiens walleriana) u uslovima suše izazvane polietilen glikolom in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5398
ER  - 

@conference{
author = "Milošević, Snežana and Đurić, Marija and Trifunović-Momčilov, Milana and Stamenković, Nikola and Marković, Marija and Subotić, Angelina",
year = "2022",
abstract = "Лепи Јова (Impatiens walleriana) је веома популарна хортикултурна врста у Србији 
и свету због својих декоративних особина, дугог периода цветања и широког 
спектра боје цветова. У његовој комерцијалној производњи наводњавање је 
смањено током транспорта и пласмана биљака на тржиште. Суша има неповољан 
утицај на растење и развиће биљака, а у ћелијама доводи до успоравања 
анаболичких и убрзавања катаболичких процеса, уз појачану производњу 
реактивних форми кисеоника (Reactive oxygen species – ROS). Дисбаланс између 
настанка и уклањања ROS има за последицу нарушавање ћелијске хомеостазе и 
оксидативни стрес. Иако ROS имају важну сигналну улогу, њихова висока 
цитотоксичност и реактивност доводе до модификација DNK, RNK, протеина и 
липида мембрана. У овом истраживању испитиван је утицај натријум 
нитропрусида (Sodium nitroprusside – SNP), као донора NO, на физиолошке и 
биохемијске промене in vitro гајеног Лепог Јове у условима суше изазване 
полиетилен гликолом (PEG8000). Изданци (величине 20-25 мм) су гајени на 
хранљивим подлогама са SNP (0, 50, 100 и 250 μM) 20 дана. Након претретмана, 
изданци су пребачени на свеже MS подлоге којима су додати PEG8000 (0 или 3%) 
и/или SNP (0, 50, 100 или 250 μM) и гајени су седам дана. Утицај егзогене примене 
SNP на повећање толеранције Лепог Јове на сушу испољава се у променама 
концентрације H2O2, малондиалдехида и укупних фенолних једињења, као и 
активности актиоксидативних ензима: каталаза, пероксидаза и супероксиддисмутаза у изданцима., Lepi Jova (Impatiens walleriana) je veoma popularna hortikulturna vrsta u Srbiji i svetu zbog svojih dekorativnih osobina, dugog perioda cvetanja i širokog spektra boje cvetova. U njegovoj komercijalnoj proizvodnji navodnjavanje je smanjeno tokom transporta i plasmana biljaka na tržište. Suša ima nepovoljan uticaj na rastenje i razviće biljaka, a u ćelijama dovodi do usporavanja anaboličkih i ubrzavanja kataboličkih procesa, uz pojačanu proizvodnju reaktivnih formi kiseonika (Reactive oxygen species – ROS). Disbalans između nastanka i uklanjanja ROS ima za posledicu narušavanje ćelijske homeostaze i oksidativni stres. Iako ROS imaju važnu signalnu ulogu, njihova visoka citotoksičnost i reaktivnost dovode do modifikacija DNK, RNK, proteina i lipida membrana. U ovom istraživanju ispitivan je uticaj natrijum nitroprusida (Sodium nitroprusside – SNP), kao donora NO, na fiziološke i biohemijske promene in vitro gajenog Lepog Jove u uslovima suše izazvane polietilen glikolom (PEG8000). Izdanci (veličine 20-25 mm) su gajeni na hranljivim podlogama sa SNP (0, 50, 100 i 250 μM) 20 dana. Nakon pretretmana, izdanci su prebačeni na sveže MS podloge kojima su dodati PEG8000 (0 ili 3%) i/ili SNP (0, 50, 100 ili 250 μM) i gajeni su sedam dana. Uticaj egzogene primene SNP na povećanje tolerancije Lepog Jove na sušu ispoljava se u promenama koncentracije H2O2, malondialdehida i ukupnih fenolnih jedinjenja, kao i aktivnosti aktioksidativnih enzima: katalaza, peroksidaza i superoksiddismutaza u izdancima.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro, Uticaj natrijum nitroprusida na fiziološki odgovor Lepog Jove (Impatiens walleriana) u uslovima suše izazvane polietilen glikolom in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5398"
}

Milošević, S., Đurić, M., Trifunović-Momčilov, M., Stamenković, N., Marković, M.,& Subotić, A.. (2022). Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5398

Milošević S, Đurić M, Trifunović-Momčilov M, Stamenković N, Marković M, Subotić A. Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5398 .

Milošević, Snežana, Đurić, Marija, Trifunović-Momčilov, Milana, Stamenković, Nikola, Marković, Marija, Subotić, Angelina, "Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5398 .

TY  - CONF
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Đurić, Marija
AU  - Koruga, Đuro
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5395
AB  - Нанотехнологија је релативно нова научна област и неопходна су темељнија 
истраживања да би се помоћу ње могло доћи до корисних сазнања за добробит 
човечанства. Многе земље у свету су свесне потенцијала који пружа примена 
нанотехнологије у пољопривреди и развијају програме који би омогућили 
повећање приноса, заштиту околине од прекомерне употребе хербицида и 
пестицида и производњу функционалне хране. Циљ ових истраживања је био да се 
испита ефекат 200 mg/l 3HWFC+
наносупстанце (патент PCT/EP2019/083307) на 
процес клијања, морфолошке и физиолошке аспекте развића плодова парадајза. У 
фази развића клијанаца успешно је стимулисан пораст биомасе у присуству 
наносупстанце. У овом раду приказан је и позитиван утицај наносупстанце на 
развиће плодова парадајза и њихов квалитет. У погледу квалитета плодова, 
најбољи резултат ефеката наносусптанце огледао се у повећању садржаја ликопена 
код зрелих плодова у односу на плодове контролних биљака. Резултати ових 
истраживања указују на нови научни допринос разумевању ефекта 3HFWC+
наносупстанце на развојне процесе парадајза и такође имају потенцијални значај у 
унапређењу технологије производње ове повртарске културе, односно добијању 
плодова са повећаним садржајем ликопена.
AB  - Nanotehnologija je relativno nova naučna oblast i neophodna su temeljnija istraživanja da bi se pomoću nje moglo doći do korisnih saznanja za dobrobit čovečanstva. Mnoge zemlje u svetu su svesne potencijala koji pruža primena nanotehnologije u poljoprivredi i razvijaju programe koji bi omogućili povećanje prinosa, zaštitu okoline od prekomerne upotrebe herbicida i pesticida i proizvodnju funkcionalne hrane. Cilj ovih istraživanja je bio da se ispita efekat 200 mg/l 3HWFC+ nanosupstance (patent PCT/EP2019/083307) na proces klijanja, morfološke i fiziološke aspekte razvića plodova paradajza. U fazi razvića klijanaca uspešno je stimulisan porast biomase u prisustvu nanosupstance. U ovom radu prikazan je i pozitivan uticaj nanosupstance na razviće plodova paradajza i njihov kvalitet. U pogledu kvaliteta plodova, najbolji rezultat efekata nanosusptance ogledao se u povećanju sadržaja likopena kod zrelih plodova u odnosu na plodove kontrolnih biljaka. Rezultati ovih istraživanja ukazuju na novi naučni doprinos razumevanju efekta 3HFWC+ nanosupstance na razvojne procese paradajza i takođe imaju potencijalni značaj u unapređenju tehnologije proizvodnje ove povrtarske kulture, odnosno dobijanju plodova sa povećanim sadržajem likopena.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.)
T1  - Efekat drugog derivata molekula S60, 3HFWC+ nanosupstance, na morfološke i fiziološke aspekte razvića paradajza (Solanum lycopersicum L.)
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5395
ER  - 

@conference{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana and Jevremović, Slađana and Đurić, Marija and Koruga, Đuro",
year = "2022",
abstract = "Нанотехнологија је релативно нова научна област и неопходна су темељнија 
истраживања да би се помоћу ње могло доћи до корисних сазнања за добробит 
човечанства. Многе земље у свету су свесне потенцијала који пружа примена 
нанотехнологије у пољопривреди и развијају програме који би омогућили 
повећање приноса, заштиту околине од прекомерне употребе хербицида и 
пестицида и производњу функционалне хране. Циљ ових истраживања је био да се 
испита ефекат 200 mg/l 3HWFC+
наносупстанце (патент PCT/EP2019/083307) на 
процес клијања, морфолошке и физиолошке аспекте развића плодова парадајза. У 
фази развића клијанаца успешно је стимулисан пораст биомасе у присуству 
наносупстанце. У овом раду приказан је и позитиван утицај наносупстанце на 
развиће плодова парадајза и њихов квалитет. У погледу квалитета плодова, 
најбољи резултат ефеката наносусптанце огледао се у повећању садржаја ликопена 
код зрелих плодова у односу на плодове контролних биљака. Резултати ових 
истраживања указују на нови научни допринос разумевању ефекта 3HFWC+
наносупстанце на развојне процесе парадајза и такође имају потенцијални значај у 
унапређењу технологије производње ове повртарске културе, односно добијању 
плодова са повећаним садржајем ликопена., Nanotehnologija je relativno nova naučna oblast i neophodna su temeljnija istraživanja da bi se pomoću nje moglo doći do korisnih saznanja za dobrobit čovečanstva. Mnoge zemlje u svetu su svesne potencijala koji pruža primena nanotehnologije u poljoprivredi i razvijaju programe koji bi omogućili povećanje prinosa, zaštitu okoline od prekomerne upotrebe herbicida i pesticida i proizvodnju funkcionalne hrane. Cilj ovih istraživanja je bio da se ispita efekat 200 mg/l 3HWFC+ nanosupstance (patent PCT/EP2019/083307) na proces klijanja, morfološke i fiziološke aspekte razvića plodova paradajza. U fazi razvića klijanaca uspešno je stimulisan porast biomase u prisustvu nanosupstance. U ovom radu prikazan je i pozitivan uticaj nanosupstance na razviće plodova paradajza i njihov kvalitet. U pogledu kvaliteta plodova, najbolji rezultat efekata nanosusptance ogledao se u povećanju sadržaja likopena kod zrelih plodova u odnosu na plodove kontrolnih biljaka. Rezultati ovih istraživanja ukazuju na novi naučni doprinos razumevanju efekta 3HFWC+ nanosupstance na razvojne procese paradajza i takođe imaju potencijalni značaj u unapređenju tehnologije proizvodnje ove povrtarske kulture, odnosno dobijanju plodova sa povećanim sadržajem likopena.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.), Efekat drugog derivata molekula S60, 3HFWC+ nanosupstance, na morfološke i fiziološke aspekte razvića paradajza (Solanum lycopersicum L.)",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5395"
}

Subotić, A., Trifunović-Momčilov, M., Milošević, S., Jevremović, S., Đurić, M.,& Koruga, Đ.. (2022). Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 49.
https://hdl.handle.net/21.15107/rcub_ibiss_5395

Subotić A, Trifunović-Momčilov M, Milošević S, Jevremović S, Đurić M, Koruga Đ. Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:49.
https://hdl.handle.net/21.15107/rcub_ibiss_5395 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, Jevremović, Slađana, Đurić, Marija, Koruga, Đuro, "Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):49,
https://hdl.handle.net/21.15107/rcub_ibiss_5395 .

TY  - CONF
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Jerinić-Prodanović, Dušanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5396
AB  - Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес.
AB  - Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica
T1  - Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5396
ER  - 

@conference{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana and Đurić, Marija and Jevremović, Slađana and Jerinić-Prodanović, Dušanka",
year = "2022",
abstract = "Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес., Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica, Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5396"
}

Subotić, A., Trifunović-Momčilov, M., Milošević, S., Đurić, M., Jevremović, S.,& Jerinić-Prodanović, D.. (2022). Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396

Subotić A, Trifunović-Momčilov M, Milošević S, Đurić M, Jevremović S, Jerinić-Prodanović D. Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, Đurić, Marija, Jevremović, Slađana, Jerinić-Prodanović, Dušanka, "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):48,
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5363
AB  - Суша представља ограничавајући фактор у производњи комерцијално значајних
биљних врста, испољавајући негативне ефекте у свим развојним стадијумима. У
овом истраживању испитиван је утицај фолијарне примене елицитора метил
јасмоната (МеЈА) на растење и физиолошки одговор Лепог Јове (Impatiens
walleriana) при суши. Лепи Јова се убраја у најпопуларније хортикултурне врсте
широм света, а ограничавајући фактор у производњи, транспорту и пласману
биљака на тржишту представља дефицит воде. Елицитација подразумева примену
једињења у циљу индуковања сигналних путева повезаних са одбраном биљака од
стреса, а у ту сврху често се користе регулатори растења попут МеЈА. Фолијарно
третирање биљака МеЈА (5 и 50 μМ) вршено је два пута током експерименталног
рада: седам дана пре индуковања суше и на дан индукције суше. Биљке су
подељене у групе према третманима, а суша је индукована тако што биљке нису
заливане до достизања жељених процената влажности супстрата (15 и 5%). Оба
третмана суше укључивала су, поред контролних биљака (35-37% влажности
супстрата) и биљке које су постепено рехидратисане – заливане четири дана након
исушивања до достизања контролних вредности влажности супстрата. Забележен
је позитиван утицај МеЈА на параметре растења: масу свежих и сувих изданака,
као и укупну површину листова Лепог Јове. Код група биљака третираних МеЈА,
забележен је пад у концентрацији показатеља оксидативног стреса –
малондиалдехида и H2О2 при суши, као и мањи садржај полифенола и флавонола.
AB  - Suša predstavlja ograničavajući faktor u proizvodnji komercijalno značajnih biljnih vrsta, ispoljavajući negativne efekte u svim razvojnim stadijumima. U ovom istraživanju ispitivan je uticaj folijarne primene elicitora metil jasmonata (MeJA) na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri suši. Lepi Jova se ubraja u najpopularnije hortikulturne vrste širom sveta, a ograničavajući faktor u proizvodnji, transportu i plasmanu biljaka na tržištu predstavlja deficit vode. Elicitacija podrazumeva primenu jedinjenja u cilju indukovanja signalnih puteva povezanih sa odbranom biljaka od stresa, a u tu svrhu često se koriste regulatori rastenja poput MeJA. Folijarno tretiranje biljaka MeJA (5 i 50 μM) vršeno je dva puta tokom eksperimentalnog rada: sedam dana pre indukovanja suše i na dan indukcije suše. Biljke su podeljene u grupe prema tretmanima, a suša je indukovana tako što biljke nisu zalivane do dostizanja željenih procenata vlažnosti supstrata (15 i 5%). Oba tretmana suše uključivala su, pored kontrolnih biljaka (35-37% vlažnosti supstrata) i biljke koje su postepeno rehidratisane – zalivane četiri dana nakon isušivanja do dostizanja kontrolnih vrednosti vlažnosti supstrata. Zabeležen je pozitivan uticaj MeJA na parametre rastenja: masu svežih i suvih izdanaka, kao i ukupnu površinu listova Lepog Jove. Kod grupa biljaka tretiranih MeJA, zabeležen je pad u koncentraciji pokazatelja oksidativnog stresa – malondialdehida i H2O2 pri suši, kao i manji sadržaj polifenola i flavonola.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши
T1  - Uticaj folijarne primene metil jasmonata na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri indukovanoj suši
IS  - 048
VL  - 57
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5363
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2022",
abstract = "Суша представља ограничавајући фактор у производњи комерцијално значајних
биљних врста, испољавајући негативне ефекте у свим развојним стадијумима. У
овом истраживању испитиван је утицај фолијарне примене елицитора метил
јасмоната (МеЈА) на растење и физиолошки одговор Лепог Јове (Impatiens
walleriana) при суши. Лепи Јова се убраја у најпопуларније хортикултурне врсте
широм света, а ограничавајући фактор у производњи, транспорту и пласману
биљака на тржишту представља дефицит воде. Елицитација подразумева примену
једињења у циљу индуковања сигналних путева повезаних са одбраном биљака од
стреса, а у ту сврху често се користе регулатори растења попут МеЈА. Фолијарно
третирање биљака МеЈА (5 и 50 μМ) вршено је два пута током експерименталног
рада: седам дана пре индуковања суше и на дан индукције суше. Биљке су
подељене у групе према третманима, а суша је индукована тако што биљке нису
заливане до достизања жељених процената влажности супстрата (15 и 5%). Оба
третмана суше укључивала су, поред контролних биљака (35-37% влажности
супстрата) и биљке које су постепено рехидратисане – заливане четири дана након
исушивања до достизања контролних вредности влажности супстрата. Забележен
је позитиван утицај МеЈА на параметре растења: масу свежих и сувих изданака,
као и укупну површину листова Лепог Јове. Код група биљака третираних МеЈА,
забележен је пад у концентрацији показатеља оксидативног стреса –
малондиалдехида и H2О2 при суши, као и мањи садржај полифенола и флавонола., Suša predstavlja ograničavajući faktor u proizvodnji komercijalno značajnih biljnih vrsta, ispoljavajući negativne efekte u svim razvojnim stadijumima. U ovom istraživanju ispitivan je uticaj folijarne primene elicitora metil jasmonata (MeJA) na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri suši. Lepi Jova se ubraja u najpopularnije hortikulturne vrste širom sveta, a ograničavajući faktor u proizvodnji, transportu i plasmanu biljaka na tržištu predstavlja deficit vode. Elicitacija podrazumeva primenu jedinjenja u cilju indukovanja signalnih puteva povezanih sa odbranom biljaka od stresa, a u tu svrhu često se koriste regulatori rastenja poput MeJA. Folijarno tretiranje biljaka MeJA (5 i 50 μM) vršeno je dva puta tokom eksperimentalnog rada: sedam dana pre indukovanja suše i na dan indukcije suše. Biljke su podeljene u grupe prema tretmanima, a suša je indukovana tako što biljke nisu zalivane do dostizanja željenih procenata vlažnosti supstrata (15 i 5%). Oba tretmana suše uključivala su, pored kontrolnih biljaka (35-37% vlažnosti supstrata) i biljke koje su postepeno rehidratisane – zalivane četiri dana nakon isušivanja do dostizanja kontrolnih vrednosti vlažnosti supstrata. Zabeležen je pozitivan uticaj MeJA na parametre rastenja: masu svežih i suvih izdanaka, kao i ukupnu površinu listova Lepog Jove. Kod grupa biljaka tretiranih MeJA, zabeležen je pad u koncentraciji pokazatelja oksidativnog stresa – malondialdehida i H2O2 pri suši, kao i manji sadržaj polifenola i flavonola.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши, Uticaj folijarne primene metil jasmonata na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri indukovanoj suši",
number = "048",
volume = "57",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5363"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2022). Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 57(048), 64.
https://hdl.handle.net/21.15107/rcub_ibiss_5363

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;57(048):64.
https://hdl.handle.net/21.15107/rcub_ibiss_5363 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia, 57, no. 048 (2022):64,
https://hdl.handle.net/21.15107/rcub_ibiss_5363 .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Milošević, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4119
AB  - Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
PB  - Basel, Switzerland: MDPI
T2  - Plants
T1  - Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery
IS  - 1
VL  - 10
DO  - 10.3390/plants10010154
SP  - 154
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Dragićević, Milan and Simonović, Ana and Milošević, Snežana",
year = "2021",
abstract = "Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.",
publisher = "Basel, Switzerland: MDPI",
journal = "Plants",
title = "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery",
number = "1",
volume = "10",
doi = "10.3390/plants10010154",
pages = "154"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Dragićević, M., Simonović, A.,& Milošević, S.. (2021). Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants
Basel, Switzerland: MDPI., 10(1), 154.
https://doi.org/10.3390/plants10010154

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Dragićević M, Simonović A, Milošević S. Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants. 2021;10(1):154.
doi:10.3390/plants10010154 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Dragićević, Milan, Simonović, Ana, Milošević, Snežana, "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery" in Plants, 10, no. 1 (2021):154,
https://doi.org/10.3390/plants10010154 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4501
AB  - The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro
IS  - 10
VL  - 11
DO  - 10.3390/agronomy11102056
SP  - 2056
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Đurić, Marija and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro",
number = "10",
volume = "11",
doi = "10.3390/agronomy11102056",
pages = "2056"
}

Trifunović-Momčilov, M., Milošević, S., Marković, M., Đurić, M., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy
Basel: MDPI., 11(10), 2056.
https://doi.org/10.3390/agronomy11102056

Trifunović-Momčilov M, Milošević S, Marković M, Đurić M, Jevremović S, Dragićević IČ, Subotić A. Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy. 2021;11(10):2056.
doi:10.3390/agronomy11102056 .

Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Đurić, Marija, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro" in Agronomy, 11, no. 10 (2021):2056,
https://doi.org/10.3390/agronomy11102056 . .

TY  - CONF
AU  - Jerinić-Prodanović, Dušanka
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4905
AB  - Bactericera trigonica je jedna od malobrojnih štetočina koje se javljaju na gajenim
vrstama iz familije Apiaceae. Godišnje može da razvije i do devet generacija, a prisustvo svih
stadijuma razvića na gajenim biljkama je potvrđeno tokom cele vegetacije. Ishranom imaga i
larve ne dovode do vidljivih simptoma oštećenja na napadnutim biljkama. Ukoliko se jave u
povećanoj brojnosti mogu prouzrokovati sušenje pojedinih delova ili čak celih biljaka. Pored
direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Ca. Liberibacter
solanacearum sa zaraženih na zdrave biljke šargarepe i celera. U prirodnim uslovima, kao i pri
uzgoju na poljoprivrednim površinama, biljke su tokom svog životnog ciklusa izložene
promjenjivim uslovima životne sredine koji mogu na različite načine delovati na njihovo
razviće i rastenje. Biotski faktori, poput napada insekata izazivaju promene na svim nivoima
organizacije biljke, od anatomskih i morfoloških osobina do ćelijskog, biohemijskog i
molekularnog nivoa. U eksperiment su bile uključene biljke šargarepe (Daucus carota),
peršuna (Pastinaca sativa) i celera (Apium graveolens), koje smo gajili u saksijama u fitotronu
u kontrolisanim uslovima. U svakoj saksiji se nalazilo po tri biljke. Imaga B. trigonica smo
sakupljali u usevu šargarepe u Futogu, a zatim smo odgajili jednu generaciju na šargarepi u
laboratoriji pri sobnoj temperaturi. Od odgajenih imaga B. trigonica smo formnirali parove po
tri mužjaka i tri ženke, koje smo nanosili na predhodno izolovane biljke sa cilidrima od
markizeta. Ovako izolovane biljke sa nanetim lisnim buvama smo gajili na sobnoj temperaturi.
Radi analize fizioloških i biohemijskih odgovora biljaka, nastalih ishranom imaga i larvi lisne
buve, uzimani su uzorci listova sa svih biljaka. Prvo uzorkovanje je bilo nakon nanošenja
imaga lisne buve, a drugo kada su na biljkama masovno bile larve. Kod biljaka su praćeni
fiziološki parametri (sadržaj fotosintetičkih pigmenata, hlorofila i karotenoida) i biohemijski
parametri (stepen lipidne peroksidacije (MDA), koncetracija vodonik-peroksida (H2O2),
aktivnost oksidativnih enzima, peroksidaza (POX), katalaza (CAT) i super-okisid dismutaza
(SOD)), kao odgovori biljaka na stres indukovan ishranom imaga i larvi lisne buve. Usled
povreda nastalih ishranom lisne buve kod svih eksperimentalnih biljaka, došlo je do
poremećaja homeostaze fotosintetičkih pigmenta. Najveće promene su uočene u sadržaju
karotenoida, koji predstavljaju važnu komponentnu u neenzimskom odgovoru biljaka na
stres. Koncentracija vodonik-peroksida i stepen lipidne peroksidacije, kod peršuna i
šargarepe, koje su inokulirane imagom, se smanjuju, dok kod celera se ispoljava suprotan
efekat. U početnoj fazi ishrane imaga lisne buve inicijalno povećanje reaktivnih formi kiseonika se neutrališe efikasnom enzimskom antioksidativnom zaštitom, što se ogleda u
povećanoj aktivnosti enzima POX, CAT i SOD, naročito kod peršuna.
PB  - Belgrade: The Plant Protection Society of Serbia
C3  - Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia
T1  - Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea)
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4905
ER  - 

@conference{
author = "Jerinić-Prodanović, Dušanka and Milošević, Snežana and Đurić, Marija and Jevremović, Slađana and Subotić, Angelina",
year = "2021",
abstract = "Bactericera trigonica je jedna od malobrojnih štetočina koje se javljaju na gajenim
vrstama iz familije Apiaceae. Godišnje može da razvije i do devet generacija, a prisustvo svih
stadijuma razvića na gajenim biljkama je potvrđeno tokom cele vegetacije. Ishranom imaga i
larve ne dovode do vidljivih simptoma oštećenja na napadnutim biljkama. Ukoliko se jave u
povećanoj brojnosti mogu prouzrokovati sušenje pojedinih delova ili čak celih biljaka. Pored
direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Ca. Liberibacter
solanacearum sa zaraženih na zdrave biljke šargarepe i celera. U prirodnim uslovima, kao i pri
uzgoju na poljoprivrednim površinama, biljke su tokom svog životnog ciklusa izložene
promjenjivim uslovima životne sredine koji mogu na različite načine delovati na njihovo
razviće i rastenje. Biotski faktori, poput napada insekata izazivaju promene na svim nivoima
organizacije biljke, od anatomskih i morfoloških osobina do ćelijskog, biohemijskog i
molekularnog nivoa. U eksperiment su bile uključene biljke šargarepe (Daucus carota),
peršuna (Pastinaca sativa) i celera (Apium graveolens), koje smo gajili u saksijama u fitotronu
u kontrolisanim uslovima. U svakoj saksiji se nalazilo po tri biljke. Imaga B. trigonica smo
sakupljali u usevu šargarepe u Futogu, a zatim smo odgajili jednu generaciju na šargarepi u
laboratoriji pri sobnoj temperaturi. Od odgajenih imaga B. trigonica smo formnirali parove po
tri mužjaka i tri ženke, koje smo nanosili na predhodno izolovane biljke sa cilidrima od
markizeta. Ovako izolovane biljke sa nanetim lisnim buvama smo gajili na sobnoj temperaturi.
Radi analize fizioloških i biohemijskih odgovora biljaka, nastalih ishranom imaga i larvi lisne
buve, uzimani su uzorci listova sa svih biljaka. Prvo uzorkovanje je bilo nakon nanošenja
imaga lisne buve, a drugo kada su na biljkama masovno bile larve. Kod biljaka su praćeni
fiziološki parametri (sadržaj fotosintetičkih pigmenata, hlorofila i karotenoida) i biohemijski
parametri (stepen lipidne peroksidacije (MDA), koncetracija vodonik-peroksida (H2O2),
aktivnost oksidativnih enzima, peroksidaza (POX), katalaza (CAT) i super-okisid dismutaza
(SOD)), kao odgovori biljaka na stres indukovan ishranom imaga i larvi lisne buve. Usled
povreda nastalih ishranom lisne buve kod svih eksperimentalnih biljaka, došlo je do
poremećaja homeostaze fotosintetičkih pigmenta. Najveće promene su uočene u sadržaju
karotenoida, koji predstavljaju važnu komponentnu u neenzimskom odgovoru biljaka na
stres. Koncentracija vodonik-peroksida i stepen lipidne peroksidacije, kod peršuna i
šargarepe, koje su inokulirane imagom, se smanjuju, dok kod celera se ispoljava suprotan
efekat. U početnoj fazi ishrane imaga lisne buve inicijalno povećanje reaktivnih formi kiseonika se neutrališe efikasnom enzimskom antioksidativnom zaštitom, što se ogleda u
povećanoj aktivnosti enzima POX, CAT i SOD, naročito kod peršuna.",
publisher = "Belgrade: The Plant Protection Society of Serbia",
journal = "Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia",
title = "Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea)",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4905"
}

Jerinić-Prodanović, D., Milošević, S., Đurić, M., Jevremović, S.,& Subotić, A.. (2021). Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea). in Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia
Belgrade: The Plant Protection Society of Serbia., 57.
https://hdl.handle.net/21.15107/rcub_ibiss_4905

Jerinić-Prodanović D, Milošević S, Đurić M, Jevremović S, Subotić A. Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea). in Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia. 2021;:57.
https://hdl.handle.net/21.15107/rcub_ibiss_4905 .

Jerinić-Prodanović, Dušanka, Milošević, Snežana, Đurić, Marija, Jevremović, Slađana, Subotić, Angelina, "Fiziološki i biohemijski odgovori šargarepe, peršuna i celera izazvani ishranom mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera: Psylloidea)" in Zbornik rezimea radova: XVI savetovanje o zaštiti bilja; 2021 Feb 22-25; Zlatibor, Serbia (2021):57,
https://hdl.handle.net/21.15107/rcub_ibiss_4905 .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Vujičić, Milorad
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4898
AB  - Impatiens walleriana (Balsaminaceae) is a worldwide popular horticultural plant and commercially produced in Serbia for many years. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in the cells turgor pressure and tissue dehydration. This is the main problem in commercial production of these plants, especially during transport and sale process when plants are not always sufficiently supplied with water. We assessed the drought and recovery effect on growth, physiological and biochemical parameters in I. walleriana (Xtreme Scarlet) grown in chamber under controlled physical conditions (ex vitro). Plants were 74 days old when the drought stress was imposed. We examined: control plants – grown under optimal watering (37%-40% of soil moisture content), drought stressed plants – (25%, 15%, 10% and 5% of soil moisture content), and recovery plants – rehydrated for four days to reach optimum soil moisture content (37%-40%). Drought reduces fresh (FW) and dry weight (DW) of shoots, as well as total leaf area and shoots water potential. Drought significantly increased abscisic acid (ABA) content in the leaves in order to reduce transpiration rate and preserve water in it᾽s tissue. The concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were increased in drought-stressed plants, as a consequence of drought induced oxidative stress in the plant cells. The activity of antioxidant enzymes (superoxide dismutase - SOD, peroxidase - POX and catalase - CAT) contributed to neutralizing negative effects of oxidative stress on plant growth, while the different SOD and POX isoforms were detected on gel. Recovery treatment neutralised negative effect of drought on growth, physiological and biochemical parameters analysed in I. walleriana to a certain extent.
PB  - Brussels: European Plant Science Organisation (EPSO)
C3  - Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy
T1  - Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro
SP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4898
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Vujičić, Milorad and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Milošević, Snežana",
year = "2021",
abstract = "Impatiens walleriana (Balsaminaceae) is a worldwide popular horticultural plant and commercially produced in Serbia for many years. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in the cells turgor pressure and tissue dehydration. This is the main problem in commercial production of these plants, especially during transport and sale process when plants are not always sufficiently supplied with water. We assessed the drought and recovery effect on growth, physiological and biochemical parameters in I. walleriana (Xtreme Scarlet) grown in chamber under controlled physical conditions (ex vitro). Plants were 74 days old when the drought stress was imposed. We examined: control plants – grown under optimal watering (37%-40% of soil moisture content), drought stressed plants – (25%, 15%, 10% and 5% of soil moisture content), and recovery plants – rehydrated for four days to reach optimum soil moisture content (37%-40%). Drought reduces fresh (FW) and dry weight (DW) of shoots, as well as total leaf area and shoots water potential. Drought significantly increased abscisic acid (ABA) content in the leaves in order to reduce transpiration rate and preserve water in it᾽s tissue. The concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were increased in drought-stressed plants, as a consequence of drought induced oxidative stress in the plant cells. The activity of antioxidant enzymes (superoxide dismutase - SOD, peroxidase - POX and catalase - CAT) contributed to neutralizing negative effects of oxidative stress on plant growth, while the different SOD and POX isoforms were detected on gel. Recovery treatment neutralised negative effect of drought on growth, physiological and biochemical parameters analysed in I. walleriana to a certain extent.",
publisher = "Brussels: European Plant Science Organisation (EPSO)",
journal = "Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy",
title = "Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro",
pages = "240",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4898"
}

Đurić, M., Subotić, A., Prokić, L., Vujičić, M., Trifunović-Momčilov, M., Cingel, A.,& Milošević, S.. (2021). Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro. in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy
Brussels: European Plant Science Organisation (EPSO)., 240.
https://hdl.handle.net/21.15107/rcub_ibiss_4898

Đurić M, Subotić A, Prokić L, Vujičić M, Trifunović-Momčilov M, Cingel A, Milošević S. Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro. in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy. 2021;:240.
https://hdl.handle.net/21.15107/rcub_ibiss_4898 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Vujičić, Milorad, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Milošević, Snežana, "Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro" in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy (2021):240,
https://hdl.handle.net/21.15107/rcub_ibiss_4898 .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Vujičić, Milorad
AU  - Milošević, Snežana
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1559
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4008
AB  - This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.
PB  - MDPI AG
T2  - Plants
T1  - Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro
IS  - 11
VL  - 9
DO  - 10.3390/plants9111559
SP  - 1559
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Vujičić, Milorad and Milošević, Snežana",
year = "2020",
abstract = "This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.",
publisher = "MDPI AG",
journal = "Plants",
title = "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro",
number = "11",
volume = "9",
doi = "10.3390/plants9111559",
pages = "1559"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Vujičić, M.,& Milošević, S.. (2020). Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. in Plants
MDPI AG., 9(11), 1559.
https://doi.org/10.3390/plants9111559

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Vujičić M, Milošević S. Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. in Plants. 2020;9(11):1559.
doi:10.3390/plants9111559 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Vujičić, Milorad, Milošević, Snežana, "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro" in Plants, 9, no. 11 (2020):1559,
https://doi.org/10.3390/plants9111559 . .