TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6372
AB  - French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.
PB  - Springer Nature
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil
DO  - 10.1007/s00344-023-11131-8
ER  - 

@article{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.",
publisher = "Springer Nature",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil",
doi = "10.1007/s00344-023-11131-8"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation
Springer Nature..
https://doi.org/10.1007/s00344-023-11131-8

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation. 2023;.
doi:10.1007/s00344-023-11131-8 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil" in Journal of Plant Growth Regulation (2023),
https://doi.org/10.1007/s00344-023-11131-8 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5856
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research
IS  - 6
VL  - 9
DO  - 10.3390/horticulturae9060674
SP  - 674
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Motyka, Vaclav and Cingel, Aleksandar and Ninković, Slavica",
year = "2023",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research",
number = "6",
volume = "9",
doi = "10.3390/horticulturae9060674",
pages = "674"
}

Ćosić, T., Raspor, M., Motyka, V., Cingel, A.,& Ninković, S.. (2023). In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae
Basel: MDPI., 9(6), 674.
https://doi.org/10.3390/horticulturae9060674

Ćosić T, Raspor M, Motyka V, Cingel A, Ninković S. In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae. 2023;9(6):674.
doi:10.3390/horticulturae9060674 .

Ćosić, Tatjana, Raspor, Martin, Motyka, Vaclav, Cingel, Aleksandar, Ninković, Slavica, "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research" in Horticulturae, 9, no. 6 (2023):674,
https://doi.org/10.3390/horticulturae9060674 . .

TY  - CONF
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5049
AB  - French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
T1  - Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5049
ER  - 

@conference{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022",
title = "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5049"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2022). Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
Belgrade: Serbian Plant Physiology Society., 76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022. 2022;:76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil" in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022 (2022):76,
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

TY  - JOUR
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
AU  - Motyka, Vaclav
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/15/1982
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9370364
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5103
AB  - This study presents the hypocotyl elongation of sunflower seedlings germinated under different light conditions. Elongation was rhythmic under diurnal (LD) photoperiods but uniform (arrhythmic) under free-running conditions of white light (LL) or darkness (DD). On the sixth day after the onset of germination, seedlings were entrained in all diurnal photoperiods. Their hypocotyl elongation was dual, showing different kinetics in daytime and nighttime periods. The daytime elongation peak was around midday and 1-2 h after dusk in the nighttime. Plantlets compensated for the differences in the daytime and nighttime durations and exhibited similar overall elongation rates, centered around the uniform elongation in LL conditions. Thus, plants from diurnal photoperiods and LL could be grouped together as white-light treatments that suppressed hypocotyl elongation. Hypocotyl elongation was significantly higher under DD than under white-light photoperiods. In continuous monochromatic blue, yellow, green, or red light, hypocotyl elongation was also uniform and very high. The treatments with monochromatic light and DD had similar overall elongation rates; thus, they could be grouped together. Compared with white light, monochromatic light promoted hypocotyl elongation. Suppression of hypocotyl elongation and rhythmicity reappeared in some combination with two or more monochromatic light colors. The presence of red light was obligatory for this suppression. Plantlets entrained in diurnal photoperiods readily slipped from rhythmic into uniform elongation if they encountered any kind of free-running conditions. These transitions occurred whenever the anticipated duration of daytime or nighttime was extended more than expected, or when plantlets were exposed to constant monochromatic light. This study revealed significant differences in the development of sunflower plantlets illuminated with monochromatic or white light.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.
IS  - 15
VL  - 11
DO  - 10.3390/plants11151982
SP  - 1982
ER  - 

@article{
author = "Vinterhalter, Dragan and Vinterhalter, Branka and Motyka, Vaclav",
year = "2022",
abstract = "This study presents the hypocotyl elongation of sunflower seedlings germinated under different light conditions. Elongation was rhythmic under diurnal (LD) photoperiods but uniform (arrhythmic) under free-running conditions of white light (LL) or darkness (DD). On the sixth day after the onset of germination, seedlings were entrained in all diurnal photoperiods. Their hypocotyl elongation was dual, showing different kinetics in daytime and nighttime periods. The daytime elongation peak was around midday and 1-2 h after dusk in the nighttime. Plantlets compensated for the differences in the daytime and nighttime durations and exhibited similar overall elongation rates, centered around the uniform elongation in LL conditions. Thus, plants from diurnal photoperiods and LL could be grouped together as white-light treatments that suppressed hypocotyl elongation. Hypocotyl elongation was significantly higher under DD than under white-light photoperiods. In continuous monochromatic blue, yellow, green, or red light, hypocotyl elongation was also uniform and very high. The treatments with monochromatic light and DD had similar overall elongation rates; thus, they could be grouped together. Compared with white light, monochromatic light promoted hypocotyl elongation. Suppression of hypocotyl elongation and rhythmicity reappeared in some combination with two or more monochromatic light colors. The presence of red light was obligatory for this suppression. Plantlets entrained in diurnal photoperiods readily slipped from rhythmic into uniform elongation if they encountered any kind of free-running conditions. These transitions occurred whenever the anticipated duration of daytime or nighttime was extended more than expected, or when plantlets were exposed to constant monochromatic light. This study revealed significant differences in the development of sunflower plantlets illuminated with monochromatic or white light.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.",
number = "15",
volume = "11",
doi = "10.3390/plants11151982",
pages = "1982"
}

Vinterhalter, D., Vinterhalter, B.,& Motyka, V.. (2022). Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(15), 1982.
https://doi.org/10.3390/plants11151982

Vinterhalter D, Vinterhalter B, Motyka V. Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings.. in Plants (Basel, Switzerland). 2022;11(15):1982.
doi:10.3390/plants11151982 .

Vinterhalter, Dragan, Vinterhalter, Branka, Motyka, Vaclav, "Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings." in Plants (Basel, Switzerland), 11, no. 15 (2022):1982,
https://doi.org/10.3390/plants11151982 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5117
AB  - The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.
PB  - Basel: MDPI
T2  - Life
T1  - Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose
IS  - 10
VL  - 12
DO  - 10.3390/life12101585
SP  - 1585
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Sajid, Sumbal and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose",
number = "10",
volume = "12",
doi = "10.3390/life12101585",
pages = "1585"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life
Basel: MDPI., 12(10), 1585.
https://doi.org/10.3390/life12101585

Ćosić T, Motyka V, Raspor M, Sajid S, Devrnja N, Dobrev PI, Ninković S. Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life. 2022;12(10):1585.
doi:10.3390/life12101585 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Sajid, Sumbal, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose" in Life, 12, no. 10 (2022):1585,
https://doi.org/10.3390/life12101585 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6129
AB  - Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.
PB  - Olomouc: Europian Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration
SP  - 131
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6129
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2019",
abstract = "Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.",
publisher = "Olomouc: Europian Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6129"
}

Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2019). Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
Olomouc: Europian Federation of Biotechnology., 131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129

Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):131-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4318
AB  - Fluctuations in local phytohormone levels are considered to be important for initiation of developmental events in the process of plant organogenesis. Simultaneously, sugars as signaling molecules affect plant metabolism and growth, potentially interacting with hormonal regulation. Thus, the aim of presented study was to see whether there were any changes in cytokinin (CK) homeostasis in 4 different stages (T1-T4) during de novo shoot organogenesis of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings under the influence of exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), combined with high sucrose concentration. Explants grown on CK-free medium with 3% sucrose were used as control. Generally, analysis of variance showed statistically significant impact of CK treatment and sucrose concentration, as well as their interaction in all investigated stages, including seedling development before calli formation (T1 and T2), calli formation (T3) and when de novo shoots occurred (T4). The obtained CK profiles exposed remarkable increase in total CK levels in samples treated with transZ, particularly when 9% sucrose was used in nutrition medium, reaching up to 50 000 pmol/g FW. This trend was observed for all CK physiological and structural groups. On the other hand, application of a synthetic urea-type CK, TDZ, contributed to little or no increase in the endogenous CK levels regardless the sucrose concentration. Our results demonstrated that phytohormones metabolism may be triggered by sucrose signaling in kohlrabi shoot organogenesis.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts
T1  - Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4318
ER  - 

@conference{
editor = "Uzelac, Branka",
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2018",
abstract = "Fluctuations in local phytohormone levels are considered to be important for initiation of developmental events in the process of plant organogenesis. Simultaneously, sugars as signaling molecules affect plant metabolism and growth, potentially interacting with hormonal regulation. Thus, the aim of presented study was to see whether there were any changes in cytokinin (CK) homeostasis in 4 different stages (T1-T4) during de novo shoot organogenesis of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings under the influence of exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), combined with high sucrose concentration. Explants grown on CK-free medium with 3% sucrose were used as control. Generally, analysis of variance showed statistically significant impact of CK treatment and sucrose concentration, as well as their interaction in all investigated stages, including seedling development before calli formation (T1 and T2), calli formation (T3) and when de novo shoots occurred (T4). The obtained CK profiles exposed remarkable increase in total CK levels in samples treated with transZ, particularly when 9% sucrose was used in nutrition medium, reaching up to 50 000 pmol/g FW. This trend was observed for all CK physiological and structural groups. On the other hand, application of a synthetic urea-type CK, TDZ, contributed to little or no increase in the endogenous CK levels regardless the sucrose concentration. Our results demonstrated that phytohormones metabolism may be triggered by sucrose signaling in kohlrabi shoot organogenesis.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts",
title = "Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4318"
}

Uzelac, B., Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2018). Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_4318

Uzelac B, Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts. 2018;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_4318 .

Uzelac, Branka, Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, 9-12 June 2018, Belgrade, Serbia, Book of abstracts (2018):39,
https://hdl.handle.net/21.15107/rcub_ibiss_4318 .

TY  - CONF
AU  - Dragićević, Ivana
AU  - Kovačević, Milica
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
AU  - Raspor, Martin
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4320
AB  - Transformed potato plants, bearing genes for cytokinin oxidase/dehydrogenase (CKX), the key enzyme in cytokinin (CK) catabolism, represent a valuable model-system for investigations of CK role in the fine tuning of tuberization. CKs are known to affect tuber initiation in vitro, while their effects on other processes, such as tuber enlargement and dormancy break, are less clear. In this study we have investigated dynamics of tuber formation (observed every 5 days during 30-day long experimental period) and parameters of tuber enlargement (tuber diameter and mass measured at the end of experiment) under strong tuber-inducing conditions in vitro (continuous darkness and high sucrose concentration [8% w/v] in the medium) in two transformed potato lines, bearing AtCKX1 or AtCKX2 genes. As previously shown, selected transformed potato lines grown in vitro were characterized by slightly elevated (line AtCKX1-36a) or manifoldly increased (line AtCKX2-51) CKX activity, resulted in differently reduced bioactive CK contents. 6-benzyladenine (BA), being neither substrate nor inhibitor of CKX, was added to the medium (at the concentration of 1 μM) in order to investigate whether it could substitute for the lack of endogenous CKs and their effect on tuberization in AtCKX potato lines. Tuber initiation on single node stem cuttings showed different dynamics in two investigated AtCKX lines, although in both lines it was completed earlier than in non-transformed control. BA at 1 μM enhanced and accelerated tuber formation in control and AtCKX1-36a. In both AtCKX lines, tuber diameter and mass were decreased, which could be restored to control values by addition of BA. BA also shortened tuber dormancy.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia
T1  - Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4320
ER  - 

@conference{
editor = "Uzelac, Branka",
author = "Dragićević, Ivana and Kovačević, Milica and Motyka, Vaclav and Ninković, Slavica and Raspor, Martin",
year = "2018",
abstract = "Transformed potato plants, bearing genes for cytokinin oxidase/dehydrogenase (CKX), the key enzyme in cytokinin (CK) catabolism, represent a valuable model-system for investigations of CK role in the fine tuning of tuberization. CKs are known to affect tuber initiation in vitro, while their effects on other processes, such as tuber enlargement and dormancy break, are less clear. In this study we have investigated dynamics of tuber formation (observed every 5 days during 30-day long experimental period) and parameters of tuber enlargement (tuber diameter and mass measured at the end of experiment) under strong tuber-inducing conditions in vitro (continuous darkness and high sucrose concentration [8% w/v] in the medium) in two transformed potato lines, bearing AtCKX1 or AtCKX2 genes. As previously shown, selected transformed potato lines grown in vitro were characterized by slightly elevated (line AtCKX1-36a) or manifoldly increased (line AtCKX2-51) CKX activity, resulted in differently reduced bioactive CK contents. 6-benzyladenine (BA), being neither substrate nor inhibitor of CKX, was added to the medium (at the concentration of 1 μM) in order to investigate whether it could substitute for the lack of endogenous CKs and their effect on tuberization in AtCKX potato lines. Tuber initiation on single node stem cuttings showed different dynamics in two investigated AtCKX lines, although in both lines it was completed earlier than in non-transformed control. BA at 1 μM enhanced and accelerated tuber formation in control and AtCKX1-36a. In both AtCKX lines, tuber diameter and mass were decreased, which could be restored to control values by addition of BA. BA also shortened tuber dormancy.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia",
title = "Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4320"
}

Uzelac, B., Dragićević, I., Kovačević, M., Motyka, V., Ninković, S.,& Raspor, M.. (2018). Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro. in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 26.
https://hdl.handle.net/21.15107/rcub_ibiss_4320

Uzelac B, Dragićević I, Kovačević M, Motyka V, Ninković S, Raspor M. Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro. in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia. 2018;:26.
https://hdl.handle.net/21.15107/rcub_ibiss_4320 .

Uzelac, Branka, Dragićević, Ivana, Kovačević, Milica, Motyka, Vaclav, Ninković, Slavica, Raspor, Martin, "Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro" in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia (2018):26,
https://hdl.handle.net/21.15107/rcub_ibiss_4320 .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Vaclav
AU  - Dragićević, Ivana
AU  - Paunović, Danijela
AU  - Marković, Marija
AU  - Subotić, Angelina
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6322
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.
PB  - elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6322
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Motyka, Vaclav and Dragićević, Ivana and Paunović, Danijela and Marković, Marija and Subotić, Angelina",
year = "2018",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.",
publisher = "elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6322"
}

Trifunović-Momčilov, M., Motyka, V., Dragićević, I., Paunović, D., Marković, M.,& Subotić, A.. (2018). Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322

Trifunović-Momčilov M, Motyka V, Dragićević I, Paunović D, Marković M, Subotić A. Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .

Trifunović-Momčilov, Milana, Motyka, Vaclav, Dragićević, Ivana, Paunović, Danijela, Marković, Marija, Subotić, Angelina, "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):33,
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .

TY  - CONF
AU  - Trailović, Maja
AU  - Dragićević, Ivana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4321
AB  - Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK). SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija
T1  - Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro
SP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4321
ER  - 

@conference{
author = "Trailović, Maja and Dragićević, Ivana and Raspor, Martin and Motyka, Vaclav and Ninković, Slavica",
year = "2018",
abstract = "Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK). SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija",
title = "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro",
pages = "62",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4321"
}

Trailović, M., Dragićević, I., Raspor, M., Motyka, V.,& Ninković, S.. (2018). Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro. in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija
Belgrade: Serbian Biological Society., 62.
https://hdl.handle.net/21.15107/rcub_ibiss_4321

Trailović M, Dragićević I, Raspor M, Motyka V, Ninković S. Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro. in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija. 2018;:62.
https://hdl.handle.net/21.15107/rcub_ibiss_4321 .

Trailović, Maja, Dragićević, Ivana, Raspor, Martin, Motyka, Vaclav, Ninković, Slavica, "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro" in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija (2018):62,
https://hdl.handle.net/21.15107/rcub_ibiss_4321 .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Vaclav
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Petrić, Marija
AU  - Holik, Josef
AU  - Malbeck, Jiri
AU  - Dobrev, Petre I.
AU  - Dragicevic, Ivana C.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2012
AB  - The plant hormones cytokinins (CKs) regulate a number of physiological
   processes. Their homeostasis is controlled by the rate of de novo
   synthesis and the rate of catabolism. The aim of this work was to
   analyze the content of total as well as individual groups of endogenous
   CKs in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea
   Rafn.) plants grown in vitro. Transgenic CKX plants represent a suitable
   model system for studying physiological and morphological processes
   controlled by CKs. In this work we clearly demonstrate a significant
   effect of AtCKX transgenes on CK metabolism in transgenic centaury
   plants. However, shoots and roots of only one AtCKX1 line and three
   AtCKX2 lines with a significant reduction of bioactive CKs were
   obtained. We also show that changes in the CKs metabolism considerably
   affected endogenous indole-3-acetic acid (IAA) levels in plant tissues.
   All analyzed transgenic AtCKX centaury lines exhibited decreased amount
   of endogenous IAA in shoots as well as in roots. Consequently, the
   IAA/bioactive CK forms ratios showed a significant variation in the
   shoots and roots of all analyzed AtCKX centaury transformants.
T2  - Plant Cell Tissue and Organ Culture
T1  - Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro
IS  - 2
VL  - 120
DO  - 10.1007/s11240-014-0646-6
SP  - 767
EP  - 777
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Vaclav and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana and Petrić, Marija and Holik, Josef and Malbeck, Jiri and Dobrev, Petre I. and Dragicevic, Ivana C.",
year = "2015",
abstract = "The plant hormones cytokinins (CKs) regulate a number of physiological
   processes. Their homeostasis is controlled by the rate of de novo
   synthesis and the rate of catabolism. The aim of this work was to
   analyze the content of total as well as individual groups of endogenous
   CKs in AtCKX1 and AtCKX2-overexpressing centaury (Centaurium erythraea
   Rafn.) plants grown in vitro. Transgenic CKX plants represent a suitable
   model system for studying physiological and morphological processes
   controlled by CKs. In this work we clearly demonstrate a significant
   effect of AtCKX transgenes on CK metabolism in transgenic centaury
   plants. However, shoots and roots of only one AtCKX1 line and three
   AtCKX2 lines with a significant reduction of bioactive CKs were
   obtained. We also show that changes in the CKs metabolism considerably
   affected endogenous indole-3-acetic acid (IAA) levels in plant tissues.
   All analyzed transgenic AtCKX centaury lines exhibited decreased amount
   of endogenous IAA in shoots as well as in roots. Consequently, the
   IAA/bioactive CK forms ratios showed a significant variation in the
   shoots and roots of all analyzed AtCKX centaury transformants.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro",
number = "2",
volume = "120",
doi = "10.1007/s11240-014-0646-6",
pages = "767-777"
}

Trifunović-Momčilov, M., Motyka, V., Cingel, A., Subotić, A., Jevremović, S., Petrić, M., Holik, J., Malbeck, J., Dobrev, P. I.,& Dragicevic, I. C.. (2015). Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro. in Plant Cell Tissue and Organ Culture, 120(2), 767-777.
https://doi.org/10.1007/s11240-014-0646-6

Trifunović-Momčilov M, Motyka V, Cingel A, Subotić A, Jevremović S, Petrić M, Holik J, Malbeck J, Dobrev PI, Dragicevic IC. Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro. in Plant Cell Tissue and Organ Culture. 2015;120(2):767-777.
doi:10.1007/s11240-014-0646-6 .

Trifunović-Momčilov, Milana, Motyka, Vaclav, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, Petrić, Marija, Holik, Josef, Malbeck, Jiri, Dobrev, Petre I., Dragicevic, Ivana C., "Changes in cytokinin content and altered cytokinin homeostasis in AtCKX1
 and AtCKX2-overexpressing centaury (Centaurium erythraea Rafn.) plants
 grown in vitro" in Plant Cell Tissue and Organ Culture, 120, no. 2 (2015):767-777,
https://doi.org/10.1007/s11240-014-0646-6 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Savic, Jelena
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Travnickova, Alena
AU  - Dobrev, Petre I.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1956
AB  - Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.
T2  - Plant Cell Tissue and Organ Culture
T1  - In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0742-2
SP  - 741
EP  - 760
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Savic, Jelena and Cingel, Aleksandar and Vinterhalter, Branka and Vinterhalter, Dragan and Travnickova, Alena and Dobrev, Petre I. and Bohanec, Borut and Ninković, Slavica",
year = "2015",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.",
journal = "Plant Cell Tissue and Organ Culture",
title = "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0742-2",
pages = "741-760"
}

Ćosić, T., Motyka, V., Raspor, M., Savic, J., Cingel, A., Vinterhalter, B., Vinterhalter, D., Travnickova, A., Dobrev, P. I., Bohanec, B.,& Ninković, S.. (2015). In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture, 121(3), 741-760.
https://doi.org/10.1007/s11240-015-0742-2

Ćosić T, Motyka V, Raspor M, Savic J, Cingel A, Vinterhalter B, Vinterhalter D, Travnickova A, Dobrev PI, Bohanec B, Ninković S. In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture. 2015;121(3):741-760.
doi:10.1007/s11240-015-0742-2 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Savic, Jelena, Cingel, Aleksandar, Vinterhalter, Branka, Vinterhalter, Dragan, Travnickova, Alena, Dobrev, Petre I., Bohanec, Borut, Ninković, Slavica, "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):741-760,
https://doi.org/10.1007/s11240-015-0742-2 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Petrić, Marija P
AU  - Dragicević, Ivana C
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I
AU  - Zahajska, Lenka
AU  - Subotić, Angelina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/954
AB  - Cytokinin oxidase/dehydrogenase (CKX) is the only known enzyme involved in cytokinin catabolism. Genes coding for two Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were introduced separately into a binary cloning vector, immobilized into Agrobacterium tumefaciens strain GV3101, and introduced into root explants of centaury (Centaurium erythraea Rafn.). The integration of each transgene was confirmed by genomic PCR. Of the total transformed explants, 30 and 28.2 % of the transformants carried AtCKX1 and AtCKX2 transgenes, respectively. Of these transformants, 50 % exhibited expression of the AtCKX1 transgene, while 64 % of transformants exhibited expression of the AtCKX2 transgene. For all analysed AtCKX transgenic centaury lines, as well as for untransformed control plants, CKX activity was higher in roots than in shoots. Expression of AtCKX in most transgenic lines contributed to enhanced levels of CKX activity in root tissues; whereas, only a few lines demonstrated increased CKX activity in shoot tissues compared to those of control plants. Moreover, overexpression of AtCKX resulted in reduced morphogenetic potential in transgenic plants, but did not significantly affect biomass production in comparison to untransformed control plants.
T2  - Plant Cell Tissue and Organ Culture
T1  - Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.
IS  - 2
VL  - 115
SP  - 27
EP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_954
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Cingel, Aleksandar and Simonović, Ana and Jevremović, Slađana and Petrić, Marija P and Dragicević, Ivana C and Motyka, Vaclav and Dobrev, Petre I and Zahajska, Lenka and Subotić, Angelina",
year = "2013",
abstract = "Cytokinin oxidase/dehydrogenase (CKX) is the only known enzyme involved in cytokinin catabolism. Genes coding for two Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were introduced separately into a binary cloning vector, immobilized into Agrobacterium tumefaciens strain GV3101, and introduced into root explants of centaury (Centaurium erythraea Rafn.). The integration of each transgene was confirmed by genomic PCR. Of the total transformed explants, 30 and 28.2 % of the transformants carried AtCKX1 and AtCKX2 transgenes, respectively. Of these transformants, 50 % exhibited expression of the AtCKX1 transgene, while 64 % of transformants exhibited expression of the AtCKX2 transgene. For all analysed AtCKX transgenic centaury lines, as well as for untransformed control plants, CKX activity was higher in roots than in shoots. Expression of AtCKX in most transgenic lines contributed to enhanced levels of CKX activity in root tissues; whereas, only a few lines demonstrated increased CKX activity in shoot tissues compared to those of control plants. Moreover, overexpression of AtCKX resulted in reduced morphogenetic potential in transgenic plants, but did not significantly affect biomass production in comparison to untransformed control plants.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.",
number = "2",
volume = "115",
pages = "27-150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_954"
}

Trifunović-Momčilov, M., Cingel, A., Simonović, A., Jevremović, S., Petrić, M. P., Dragicević, I. C., Motyka, V., Dobrev, P. I., Zahajska, L.,& Subotić, A.. (2013). Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.. in Plant Cell Tissue and Organ Culture, 115(2), 27-150.
https://hdl.handle.net/21.15107/rcub_ibiss_954

Trifunović-Momčilov M, Cingel A, Simonović A, Jevremović S, Petrić MP, Dragicević IC, Motyka V, Dobrev PI, Zahajska L, Subotić A. Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn.. in Plant Cell Tissue and Organ Culture. 2013;115(2):27-150.
https://hdl.handle.net/21.15107/rcub_ibiss_954 .

Trifunović-Momčilov, Milana, Cingel, Aleksandar, Simonović, Ana, Jevremović, Slađana, Petrić, Marija P, Dragicević, Ivana C, Motyka, Vaclav, Dobrev, Petre I, Zahajska, Lenka, Subotić, Angelina, "Overexpression of Arabidopsis cytokinin oxidase/dehydrogenase genes AtCKX1 and AtCKX2 in transgenic Centaurium erythraea Rafn." in Plant Cell Tissue and Organ Culture, 115, no. 2 (2013):27-150,
https://hdl.handle.net/21.15107/rcub_ibiss_954 .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Zizkova, Eva
AU  - Dobrev, Petre I
AU  - Travnickova, Alena
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ninković, Slavica
AU  - Dragicević, Ivana C
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1147
AB  - Genes encoding cytokinin oxidase/dehydrogenase (CKX) enzymes have been used lately to study cytokinin homeostasis in a variety of plant species. In this study AtCKX2-overexpressing potato plants were engineered and grown in vitro as a model system to investigate the effects of altered cytokinin levels on tuber formation and tuber size. Protein extracts from shoots and roots of transformed potato plants exhibited higher CKX activity compared to control plants. Total endogenous cytokinin levels were generally not decreased in AtCKX2 overexpressors. However, levels of bioactive cytokinins were markedly lowered, which was accompanied by increased levels of O- and N-glucosides in some transgenic lines. The AtCKX2-overexpressing plants displayed reduced shoot growth but other symptoms of the "cytokinin deficiency syndrome" were not recorded. The transgenic plants were able to produce tubers in noninducing conditions. In inducing conditions they developed larger tubers than control. Tubers were also formed on a greater portion of the analyzed AtCKX2 plants, but with a lower number of tubers per plant compared to control. Taken together, our data suggest that cytokinins cannot be regarded simply as positive or negative regulators of tuberization, at least in vitro. Interactions with other plant hormones that play an important role in control of tuberization, such as gibberellins, should be further studied in detail.
T2  - Journal of Plant Growth Regulation
T1  - Cytokinin Profiles of AtCKX2-Overexpressing Potato Plants and the Impact of Altered Cytokinin Homeostasis on Tuberization In Vitro
IS  - 3
VL  - 31
SP  - 85
EP  - 470
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1147
ER  - 

@article{
author = "Raspor, Martin and Motyka, Vaclav and Zizkova, Eva and Dobrev, Petre I and Travnickova, Alena and Zdravković-Korać, Snežana and Simonović, Ana and Ninković, Slavica and Dragicević, Ivana C",
year = "2012",
abstract = "Genes encoding cytokinin oxidase/dehydrogenase (CKX) enzymes have been used lately to study cytokinin homeostasis in a variety of plant species. In this study AtCKX2-overexpressing potato plants were engineered and grown in vitro as a model system to investigate the effects of altered cytokinin levels on tuber formation and tuber size. Protein extracts from shoots and roots of transformed potato plants exhibited higher CKX activity compared to control plants. Total endogenous cytokinin levels were generally not decreased in AtCKX2 overexpressors. However, levels of bioactive cytokinins were markedly lowered, which was accompanied by increased levels of O- and N-glucosides in some transgenic lines. The AtCKX2-overexpressing plants displayed reduced shoot growth but other symptoms of the "cytokinin deficiency syndrome" were not recorded. The transgenic plants were able to produce tubers in noninducing conditions. In inducing conditions they developed larger tubers than control. Tubers were also formed on a greater portion of the analyzed AtCKX2 plants, but with a lower number of tubers per plant compared to control. Taken together, our data suggest that cytokinins cannot be regarded simply as positive or negative regulators of tuberization, at least in vitro. Interactions with other plant hormones that play an important role in control of tuberization, such as gibberellins, should be further studied in detail.",
journal = "Journal of Plant Growth Regulation",
title = "Cytokinin Profiles of AtCKX2-Overexpressing Potato Plants and the Impact of Altered Cytokinin Homeostasis on Tuberization In Vitro",
number = "3",
volume = "31",
pages = "85-470",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1147"
}

Raspor, M., Motyka, V., Zizkova, E., Dobrev, P. I., Travnickova, A., Zdravković-Korać, S., Simonović, A., Ninković, S.,& Dragicević, I. C.. (2012). Cytokinin Profiles of AtCKX2-Overexpressing Potato Plants and the Impact of Altered Cytokinin Homeostasis on Tuberization In Vitro. in Journal of Plant Growth Regulation, 31(3), 85-470.
https://hdl.handle.net/21.15107/rcub_ibiss_1147

Raspor M, Motyka V, Zizkova E, Dobrev PI, Travnickova A, Zdravković-Korać S, Simonović A, Ninković S, Dragicević IC. Cytokinin Profiles of AtCKX2-Overexpressing Potato Plants and the Impact of Altered Cytokinin Homeostasis on Tuberization In Vitro. in Journal of Plant Growth Regulation. 2012;31(3):85-470.
https://hdl.handle.net/21.15107/rcub_ibiss_1147 .

Raspor, Martin, Motyka, Vaclav, Zizkova, Eva, Dobrev, Petre I, Travnickova, Alena, Zdravković-Korać, Snežana, Simonović, Ana, Ninković, Slavica, Dragicević, Ivana C, "Cytokinin Profiles of AtCKX2-Overexpressing Potato Plants and the Impact of Altered Cytokinin Homeostasis on Tuberization In Vitro" in Journal of Plant Growth Regulation, 31, no. 3 (2012):85-470,
https://hdl.handle.net/21.15107/rcub_ibiss_1147 .

TY  - CONF
AU  - Đorđević, Tatjana
AU  - Dragicević, Ivana C
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1396
C3  - In Vitro Cellular & Developmental Biology-Animal
T1  - Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration
IS  - null
VL  - 46
EP  - S109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1396
ER  - 

@conference{
author = "Đorđević, Tatjana and Dragicević, Ivana C and Vinterhalter, Branka and Vinterhalter, Dragan V and Motyka, Vaclav and Ninković, Slavica",
year = "2010",
journal = "In Vitro Cellular & Developmental Biology-Animal",
title = "Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration",
number = "null",
volume = "46",
pages = "S109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1396"
}

Đorđević, T., Dragicević, I. C., Vinterhalter, B., Vinterhalter, D. V., Motyka, V.,& Ninković, S.. (2010). Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration. in In Vitro Cellular & Developmental Biology-Animal, 46(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1396

Đorđević T, Dragicević IC, Vinterhalter B, Vinterhalter DV, Motyka V, Ninković S. Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration. in In Vitro Cellular & Developmental Biology-Animal. 2010;46(null):null-S109.
https://hdl.handle.net/21.15107/rcub_ibiss_1396 .

Đorđević, Tatjana, Dragicević, Ivana C, Vinterhalter, Branka, Vinterhalter, Dragan V, Motyka, Vaclav, Ninković, Slavica, "Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration" in In Vitro Cellular & Developmental Biology-Animal, 46, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1396 .