TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Tolinački, Maja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6084
AB  - While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.
PB  - American Society for Microbiology
T2  - Microbiology Spectrum
T1  - Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition
IS  - 5
VL  - 11
DO  - 10.1128/spectrum.01990-23
SP  - e0199023
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Malešević, Anastasija and Glamočlija, Jasmina and Brdarić, Emilija and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Tolinački, Maja",
year = "2023",
abstract = "While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.",
publisher = "American Society for Microbiology",
journal = "Microbiology Spectrum",
title = "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition",
number = "5",
volume = "11",
doi = "10.1128/spectrum.01990-23",
pages = "e0199023"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Malešević, A., Glamočlija, J., Brdarić, E., Soković Bajić, S., Golić, N., Mirkov, I.,& Tolinački, M.. (2023). Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum
American Society for Microbiology., 11(5), e0199023.
https://doi.org/10.1128/spectrum.01990-23

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Malešević A, Glamočlija J, Brdarić E, Soković Bajić S, Golić N, Mirkov I, Tolinački M. Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum. 2023;11(5):e0199023.
doi:10.1128/spectrum.01990-23 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Malešević, Anastasija, Glamočlija, Jasmina, Brdarić, Emilija, Soković Bajić, Svetlana, Golić, Nataša, Mirkov, Ivana, Tolinački, Maja, "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition" in Microbiology Spectrum, 11, no. 5 (2023):e0199023,
https://doi.org/10.1128/spectrum.01990-23 . .

TY  - CONF
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6125
AB  - Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом.
AB  - Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
T1  - Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)
T1  - Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6125
ER  - 

@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом., Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia",
title = "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II), Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6125"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
Belgrade: Serbian Academy of Sciences and Arts..
https://hdl.handle.net/21.15107/rcub_ibiss_6125

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6125 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)" in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6125 .

TY  - CONF
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Brceski, Ilija
AU  - Tolinacki, Maja
AU  - Jovanovic, Sofija
AU  - Mileusnić, Dina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4854
AB  - Our previous research showed a higher reactivity of DA (compared to AO rats) to antigens that cause skin and lung inflammatory reactions. In order to examine the effect of strains on the immune responses of other regions, the effect of oral (in drinking water, 30 days) intake of cadmium, a known food and water contaminant, on the intestinal immune response of AO and DA rats was analyzed. Despite similar amounts of cadmium deposited in the intestines of both strains, the reduction in Lactobacillus (important for maintaining immune homeostasis in the intestine) and tissue damage (histologically and according to the marker of tissue necrosis, HMGB-1) was more pronounced in DA rats.Changes, including the activity of antioxidant defense enzymes (superoxide dismutase and catalase), increased concentrations of IFN-γ and IL-17, and no changes in IL-10, which were detected in intestinal homogenates only in DA strains, indicate a more intense intestinal inflammatory reaction. compared to AO strain. The same concentrations of cadmium detected in the main draining (mesenteric) lymph nodes (MLC) led to the induction of mRNA for metal-binding redox proteins (metallothioneins, MT) only in DA rats. The presence of a proinflammatory cytokine response (protein products and mRNA) of MLC cells, detected predominantly in DA strains, indicates a more pronounced induction of cells that produce these cytokines.Increased cell proliferation and oxidative activity of MLC cells, as well as the number of CD68 +, NKG2D + and CD11b + cells only in DA rats, with a differential change in IL-10 (decrease in DA, increase in AO) emphasizes the inflammatory character of MLC rat microenvironment of this strain. The absence of similar changes in the spleen (at the same tissue load of cadmium as MLC) indicates the influence of damaged intestinal tissue on the activity of regional lymph nodes, and the more intense response of MLC DA soy reflects greater efforts to prevent systemic immune response to changes in intestinal homeostasis.
PB  - Belgrade: Immunological Society of Serbia
C3  - VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
T1  - Differential strain impact on immune reactivity: insights from regional immune responses in rats
T1  - Diferencijalni uticaj soja na imunsku reaktivnost: uvid iz regionalnih imunskih odgovora kod pacova
SP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4854
ER  - 

@conference{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Demenesku, Jelena and Brceski, Ilija and Tolinacki, Maja and Jovanovic, Sofija and Mileusnić, Dina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Our previous research showed a higher reactivity of DA (compared to AO rats) to antigens that cause skin and lung inflammatory reactions. In order to examine the effect of strains on the immune responses of other regions, the effect of oral (in drinking water, 30 days) intake of cadmium, a known food and water contaminant, on the intestinal immune response of AO and DA rats was analyzed. Despite similar amounts of cadmium deposited in the intestines of both strains, the reduction in Lactobacillus (important for maintaining immune homeostasis in the intestine) and tissue damage (histologically and according to the marker of tissue necrosis, HMGB-1) was more pronounced in DA rats.Changes, including the activity of antioxidant defense enzymes (superoxide dismutase and catalase), increased concentrations of IFN-γ and IL-17, and no changes in IL-10, which were detected in intestinal homogenates only in DA strains, indicate a more intense intestinal inflammatory reaction. compared to AO strain. The same concentrations of cadmium detected in the main draining (mesenteric) lymph nodes (MLC) led to the induction of mRNA for metal-binding redox proteins (metallothioneins, MT) only in DA rats. The presence of a proinflammatory cytokine response (protein products and mRNA) of MLC cells, detected predominantly in DA strains, indicates a more pronounced induction of cells that produce these cytokines.Increased cell proliferation and oxidative activity of MLC cells, as well as the number of CD68 +, NKG2D + and CD11b + cells only in DA rats, with a differential change in IL-10 (decrease in DA, increase in AO) emphasizes the inflammatory character of MLC rat microenvironment of this strain. The absence of similar changes in the spleen (at the same tissue load of cadmium as MLC) indicates the influence of damaged intestinal tissue on the activity of regional lymph nodes, and the more intense response of MLC DA soy reflects greater efforts to prevent systemic immune response to changes in intestinal homeostasis.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata",
title = "Differential strain impact on immune reactivity: insights from regional immune responses in rats, Diferencijalni uticaj soja na imunsku reaktivnost: uvid iz regionalnih imunskih odgovora kod pacova",
pages = "10",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4854"
}

Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Demenesku, J., Brceski, I., Tolinacki, M., Jovanovic, S., Mileusnić, D., Kataranovski, D.,& Kataranovski, M.. (2016). Differential strain impact on immune reactivity: insights from regional immune responses in rats. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata
Belgrade: Immunological Society of Serbia., 10.
https://hdl.handle.net/21.15107/rcub_ibiss_4854

Ninkov M, Popov Aleksandrov A, Mirkov I, Demenesku J, Brceski I, Tolinacki M, Jovanovic S, Mileusnić D, Kataranovski D, Kataranovski M. Differential strain impact on immune reactivity: insights from regional immune responses in rats. in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata. 2016;:10.
https://hdl.handle.net/21.15107/rcub_ibiss_4854 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Demenesku, Jelena, Brceski, Ilija, Tolinacki, Maja, Jovanovic, Sofija, Mileusnić, Dina, Kataranovski, Dragan, Kataranovski, Milena, "Differential strain impact on immune reactivity: insights from regional immune responses in rats" in VII Naučni sastanak Društva imunologa Srbije, Belgrade, Serbia, 27-28 april 2016, Knjiga apstrakata (2016):10,
https://hdl.handle.net/21.15107/rcub_ibiss_4854 .

TY  - JOUR
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Tucović, Dina
AU  - Jovanović Stojanov, Sofija
AU  - Golic, Natasa
AU  - Tolinacki, Maja
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2016
UR  - https://www.sciencedirect.com/science/article/pii/S0278691516302423?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3378
AB  - Influence of genetic background on toxicity of oral cadmium (Cd) administration (30 days, in drinking
water; 5 ppm and 50 ppm of cadmium) was examined in Albino Oxford (AO) and Dark Agouti (DA) rats.
Similar cadmium deposition was noted in gut and draining mesenteric lymph nodes (MLN) of both
strains but intensity and/or the pattern of responses to cadmium in these tissues differ. Less intense
intestinal damage and leukocyte infiltration was observed in gut of cadmium-exposed AO rats. While
gut-associated lymph node cells of DA rats responded to cadmium with an increase of cell proliferation,
oxidative activity, IFN-g, IL-17 production and expression, no changes of these activities of MLN cells of
cadmium-treated AO rats were observed. Spleen, which accumulated cadmium comparable to MLN,
responded to metal by drop in cell viability and by reduced responsiveness of proliferation and cytokine
production to stimulation in DA rats solely, which suggest tissue dependence of cadmium effects. More
pronounced cadmium effects on MLN and spleen cells of DA rats (which accumulated similar cadmium
doses as AO rats), showed greater susceptibility of this strain to cadmium. The results presented, for the
first time, depict the influence of genetic background to effects of oral cadmium administration.
T2  - Food and Chemical Toxicology
T1  - Strain differences in toxicity of oral cadmium intake in rats
VL  - 96
DO  - 10.1016/j.fct.2016.07.021
SP  - 11
EP  - 23
ER  - 

@article{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Demenesku, Jelena and Tucović, Dina and Jovanović Stojanov, Sofija and Golic, Natasa and Tolinacki, Maja and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2016",
abstract = "Influence of genetic background on toxicity of oral cadmium (Cd) administration (30 days, in drinking
water; 5 ppm and 50 ppm of cadmium) was examined in Albino Oxford (AO) and Dark Agouti (DA) rats.
Similar cadmium deposition was noted in gut and draining mesenteric lymph nodes (MLN) of both
strains but intensity and/or the pattern of responses to cadmium in these tissues differ. Less intense
intestinal damage and leukocyte infiltration was observed in gut of cadmium-exposed AO rats. While
gut-associated lymph node cells of DA rats responded to cadmium with an increase of cell proliferation,
oxidative activity, IFN-g, IL-17 production and expression, no changes of these activities of MLN cells of
cadmium-treated AO rats were observed. Spleen, which accumulated cadmium comparable to MLN,
responded to metal by drop in cell viability and by reduced responsiveness of proliferation and cytokine
production to stimulation in DA rats solely, which suggest tissue dependence of cadmium effects. More
pronounced cadmium effects on MLN and spleen cells of DA rats (which accumulated similar cadmium
doses as AO rats), showed greater susceptibility of this strain to cadmium. The results presented, for the
first time, depict the influence of genetic background to effects of oral cadmium administration.",
journal = "Food and Chemical Toxicology",
title = "Strain differences in toxicity of oral cadmium intake in rats",
volume = "96",
doi = "10.1016/j.fct.2016.07.021",
pages = "11-23"
}

Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Demenesku, J., Tucović, D., Jovanović Stojanov, S., Golic, N., Tolinacki, M., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2016). Strain differences in toxicity of oral cadmium intake in rats. in Food and Chemical Toxicology, 96, 11-23.
https://doi.org/10.1016/j.fct.2016.07.021

Ninkov M, Popov Aleksandrov A, Mirkov I, Demenesku J, Tucović D, Jovanović Stojanov S, Golic N, Tolinacki M, Kataranovski D, Brceski I, Kataranovski M. Strain differences in toxicity of oral cadmium intake in rats. in Food and Chemical Toxicology. 2016;96:11-23.
doi:10.1016/j.fct.2016.07.021 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Demenesku, Jelena, Tucović, Dina, Jovanović Stojanov, Sofija, Golic, Natasa, Tolinacki, Maja, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Strain differences in toxicity of oral cadmium intake in rats" in Food and Chemical Toxicology, 96 (2016):11-23,
https://doi.org/10.1016/j.fct.2016.07.021 . .

TY  - JOUR
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Petrovic, Anja
AU  - Grigorov, Ilijana
AU  - Zolotarevski, Lidija
AU  - Tolinacki, Maja
AU  - Kataranovski, Dragan S.
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2364
AB  - Gastrointestinal tract is one of the main targets of cadmium (Cd), an
   important food and drinking water contaminant. In the present study, the
   effect of subchronic (30 days) oral (in water) intake of 5ppm and 50ppm
   of cadmium on immune responses in the gut was examined in rats. Cadmium
   consumption resulted in reduction of bacteria corresponding to
   Lactobacillus strain, tissue damage and intestinal inflammation
   {[}increases in high mobility group box 1 (HMGB1 molecules), superoxide
   dismutase (SOD) and catalase (CAT) activity and proinflammatory cytokine
   (TNF, IL-1 beta, IFN-gamma, IL-17) content]. Draining (mesenteric) lymph
   node (MLN) stress response was observed {[}elevation of MLN glutathione
   (GSH) and metallothionein (MT) mRNA levels] and stimulation of both
   adaptive {[}cellularity, proliferation, proinflammatory (IFN-gamma and
   IL-17) MLN cell cytokine responses] as well as innate immune activity
   (increases in numbers of NK and CD68(+) cells, oxidative activities,
   IL-1 beta). In contrast to proinflammatory milieu in MLN, decreased or
   unchanged antiinflammatory IL-10 response was observed. Stimulation of
   immune activities of MLN cells have, most probably, resulted from
   sensing of cadmium-induced tissue injury, but also from bacterial
   antigens that breached compromised intestinal barrier. These effects of
   cadmium should be taken into account when assessing dietary cadmium as
   health risk factor. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - Toxicity of oral cadmium intake: Impact on gut immunity
IS  - 2
VL  - 237
DO  - 10.1016/j.toxlet.2015.06.002
SP  - 89
EP  - 99
ER  - 

@article{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Tucović, Dina and Petrovic, Anja and Grigorov, Ilijana and Zolotarevski, Lidija and Tolinacki, Maja and Kataranovski, Dragan S. and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Gastrointestinal tract is one of the main targets of cadmium (Cd), an
   important food and drinking water contaminant. In the present study, the
   effect of subchronic (30 days) oral (in water) intake of 5ppm and 50ppm
   of cadmium on immune responses in the gut was examined in rats. Cadmium
   consumption resulted in reduction of bacteria corresponding to
   Lactobacillus strain, tissue damage and intestinal inflammation
   {[}increases in high mobility group box 1 (HMGB1 molecules), superoxide
   dismutase (SOD) and catalase (CAT) activity and proinflammatory cytokine
   (TNF, IL-1 beta, IFN-gamma, IL-17) content]. Draining (mesenteric) lymph
   node (MLN) stress response was observed {[}elevation of MLN glutathione
   (GSH) and metallothionein (MT) mRNA levels] and stimulation of both
   adaptive {[}cellularity, proliferation, proinflammatory (IFN-gamma and
   IL-17) MLN cell cytokine responses] as well as innate immune activity
   (increases in numbers of NK and CD68(+) cells, oxidative activities,
   IL-1 beta). In contrast to proinflammatory milieu in MLN, decreased or
   unchanged antiinflammatory IL-10 response was observed. Stimulation of
   immune activities of MLN cells have, most probably, resulted from
   sensing of cadmium-induced tissue injury, but also from bacterial
   antigens that breached compromised intestinal barrier. These effects of
   cadmium should be taken into account when assessing dietary cadmium as
   health risk factor. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "Toxicity of oral cadmium intake: Impact on gut immunity",
number = "2",
volume = "237",
doi = "10.1016/j.toxlet.2015.06.002",
pages = "89-99"
}

Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Tucović, D., Petrovic, A., Grigorov, I., Zolotarevski, L., Tolinacki, M., Kataranovski, D. S., Brceski, I.,& Kataranovski, M.. (2015). Toxicity of oral cadmium intake: Impact on gut immunity. in Toxicology Letters, 237(2), 89-99.
https://doi.org/10.1016/j.toxlet.2015.06.002

Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Tucović D, Petrovic A, Grigorov I, Zolotarevski L, Tolinacki M, Kataranovski DS, Brceski I, Kataranovski M. Toxicity of oral cadmium intake: Impact on gut immunity. in Toxicology Letters. 2015;237(2):89-99.
doi:10.1016/j.toxlet.2015.06.002 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Tucović, Dina, Petrovic, Anja, Grigorov, Ilijana, Zolotarevski, Lidija, Tolinacki, Maja, Kataranovski, Dragan S., Brceski, Ilija, Kataranovski, Milena, "Toxicity of oral cadmium intake: Impact on gut immunity" in Toxicology Letters, 237, no. 2 (2015):89-99,
https://doi.org/10.1016/j.toxlet.2015.06.002 . .

TY  - CONF
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Tolinacki, Maja
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4809
AB  - Introduction: Toxic metal cadmium (Cd) is important food and drinking water contaminant. The majority of ingested cadmium retained in the gastrointestinal tract (GIT) mucosa
which allocates GIT as its main target. Mechanisms of induction of intestinal inflammatory response are largely unknown.
Materials and Methods: Effect of subchronic (30 days) oral (in water) cadmium administration (5 ppm and 50 ppm) was examined in Dark Agouti (DA) and Albino Oxford (AO) rats.
Beside intestinal immune response, activity of draining mesenteric lymph node (MLN) cells, central place for induction of intestinal immune tolerance and local protective responses,
was evaluated.
Results: In both rat strains cadmium consumption resulted in reduction of bacteria (Lactobacillus strain), intestinal tissue damage, modulated antioxidant enzymes activity and
inflammation [increased proinflammatory cytokine (TNF, IL-1β, IFN-γ, IL-17) content in DA rats; increased TNF and IL-10 content in AO rats] in duodenal homogenates. Accumulation
of cadmium in MLN was followed by stress response [elevation of MLN glutathione and metallothionein mRNA levels] only in DA rats. Stimulation of both adaptive (proliferation, Th1
and Th17 cytokine response) and innate immune activities (NKG2D+, CD68+ cells, selected oxidative activities, IL-1β) in MLN was observed, more pronounced in DA compared to AO
rats.
Conclusions: Oral intake of cadmium resulted in intestinal damage, inflammation, and induction of proinflammatory milleu and innate effector cell activities in MLN. Cadmiuminduced proinflammatory responses in DA rats but discrete immune responses of AO rats imply strain-dependent effects of oral cadmium administration.
PB  - European Federation of Immunological Societies
C3  - 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.
T1  - Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes
SP  - 486
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4809
ER  - 

@conference{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Mileusnić, Dina and Tolinacki, Maja and Zolotarevski, Lidija and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Introduction: Toxic metal cadmium (Cd) is important food and drinking water contaminant. The majority of ingested cadmium retained in the gastrointestinal tract (GIT) mucosa
which allocates GIT as its main target. Mechanisms of induction of intestinal inflammatory response are largely unknown.
Materials and Methods: Effect of subchronic (30 days) oral (in water) cadmium administration (5 ppm and 50 ppm) was examined in Dark Agouti (DA) and Albino Oxford (AO) rats.
Beside intestinal immune response, activity of draining mesenteric lymph node (MLN) cells, central place for induction of intestinal immune tolerance and local protective responses,
was evaluated.
Results: In both rat strains cadmium consumption resulted in reduction of bacteria (Lactobacillus strain), intestinal tissue damage, modulated antioxidant enzymes activity and
inflammation [increased proinflammatory cytokine (TNF, IL-1β, IFN-γ, IL-17) content in DA rats; increased TNF and IL-10 content in AO rats] in duodenal homogenates. Accumulation
of cadmium in MLN was followed by stress response [elevation of MLN glutathione and metallothionein mRNA levels] only in DA rats. Stimulation of both adaptive (proliferation, Th1
and Th17 cytokine response) and innate immune activities (NKG2D+, CD68+ cells, selected oxidative activities, IL-1β) in MLN was observed, more pronounced in DA compared to AO
rats.
Conclusions: Oral intake of cadmium resulted in intestinal damage, inflammation, and induction of proinflammatory milleu and innate effector cell activities in MLN. Cadmiuminduced proinflammatory responses in DA rats but discrete immune responses of AO rats imply strain-dependent effects of oral cadmium administration.",
publisher = "European Federation of Immunological Societies",
journal = "4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.",
title = "Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes",
pages = "486",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4809"
}

Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Mileusnić, D., Tolinacki, M., Zolotarevski, L., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2015). Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.
European Federation of Immunological Societies., 486.
https://hdl.handle.net/21.15107/rcub_ibiss_4809

Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Mileusnić D, Tolinacki M, Zolotarevski L, Kataranovski D, Brceski I, Kataranovski M. Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes. in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486.. 2015;:486.
https://hdl.handle.net/21.15107/rcub_ibiss_4809 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Mileusnić, Dina, Tolinacki, Maja, Zolotarevski, Lidija, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes" in 4th European Congress of Immunology, September 6-9, 2015, Vienna, Austria, p 486. (2015):486,
https://hdl.handle.net/21.15107/rcub_ibiss_4809 .