TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - CONF
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Lukić, Tamara
AU  - Gašić, Uroš
AU  - Petrović, Luka
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6481
AB  - Nepeta L. is the largest genus of the Lamiaceae family. The species of this genus are famous
for their biopesticidal potential and medicinal properties. Within the genus, we found
considerable variability in the qualitative and quantitative content of iridoid and phenolic
compounds. Using state-of-the-art metabolomics techniques (UHPLC/LTQ-Orbitrap MS,
GC/MS, UHPLC/DAD/(+/-)HESI-MS2), we comprehensively assessed the overall diversity of iridoids and phenolics in the selected chemodiverse Nepeta taxa at the intra-species level.
To reconstruct phylogenetic relations among the selected chemodiverse taxa from across the
Nepeta genus, genomic ITS molecular marker and two maternally inherited plastid DNA
regions (trnL-trnF and rbcL) were analyzed. A consensus phylogenetic tree was constructed
to interpret the distribution of the most abundant iridoid and phenolic compounds. It was
not possible to uncover clear regularities between phylogenetic relationship and chemotype
affiliation.
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta
SP  - 78
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6481
ER  - 

@conference{
author = "Banjanac, Tijana and Šiler, Branislav and Lukić, Tamara and Gašić, Uroš and Petrović, Luka and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Milutinović, Milica and Aničić, Neda and Božunović, Jelena and Filipović, Biljana and Todorović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "Nepeta L. is the largest genus of the Lamiaceae family. The species of this genus are famous
for their biopesticidal potential and medicinal properties. Within the genus, we found
considerable variability in the qualitative and quantitative content of iridoid and phenolic
compounds. Using state-of-the-art metabolomics techniques (UHPLC/LTQ-Orbitrap MS,
GC/MS, UHPLC/DAD/(+/-)HESI-MS2), we comprehensively assessed the overall diversity of iridoids and phenolics in the selected chemodiverse Nepeta taxa at the intra-species level.
To reconstruct phylogenetic relations among the selected chemodiverse taxa from across the
Nepeta genus, genomic ITS molecular marker and two maternally inherited plastid DNA
regions (trnL-trnF and rbcL) were analyzed. A consensus phylogenetic tree was constructed
to interpret the distribution of the most abundant iridoid and phenolic compounds. It was
not possible to uncover clear regularities between phylogenetic relationship and chemotype
affiliation.",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta",
pages = "78-79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6481"
}

Banjanac, T., Šiler, B., Lukić, T., Gašić, U., Petrović, L., Matekalo, D., Skorić, M., Nestorović Živković, J., Dmitrović, S., Milutinović, M., Aničić, N., Božunović, J., Filipović, B., Todorović, M.,& Mišić, D.. (2023). Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6481

Banjanac T, Šiler B, Lukić T, Gašić U, Petrović L, Matekalo D, Skorić M, Nestorović Živković J, Dmitrović S, Milutinović M, Aničić N, Božunović J, Filipović B, Todorović M, Mišić D. Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_6481 .

Banjanac, Tijana, Šiler, Branislav, Lukić, Tamara, Gašić, Uroš, Petrović, Luka, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Milutinović, Milica, Aničić, Neda, Božunović, Jelena, Filipović, Biljana, Todorović, Miloš, Mišić, Danijela, "Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):78-79,
https://hdl.handle.net/21.15107/rcub_ibiss_6481 .

TY  - GEN
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Šiler, Branislav
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Lukić, Tamara
AU  - Todorović, Miloš
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6560
AB  - Introduction: Recent intensive research on the genus Nepeta has resulted in increasingly accumulated
information regarding the biosynthetic pathways and chemical evolution of iridoids; however, several in-
triguing aspects remain yet to be resolved. Our objective was to deeply investigate the molecular back-
ground of the diversity of iridoid compounds within the genus Nepeta, as well as regulative mechanisms
of their biosynthesis.
Methods: Leaves of greenhouse-grown plants were analysed for the composition of iridoid aglycones
(IAs) and iridoid glucosides (IGs) adopting non-targeted (UHPLC/LTQ Orbitrap MS n
, GC/MS) and targeted
(UHPLC/DAD/(±)HESI-MS 2 ) metabolomics approaches. Following RNA-Seq, transcriptomes of phylodi-
verse Nepeta taxa were searched for the presence/absence of iridoid-related biosynthetic genes. Co-ex-
pression patterns (qPCR) of biosynthetic genes and transcription factors were determined following
plants’ exposure to various environmental stresses.
Results: The comparison of metabolite composition among phylodiverse Nepeta taxa provided a sys-
tematic understanding of qualitative and quantitative composition of iridoids in leaves of the selected
Nepeta taxa. Mining of RNA-Seq data in search for iridoid biosynthesis-related genes pointed to signifi-
cant differences between taxa producing both IAs and IGs and those producing only IGs. Comparative
metabolomics and gene co-expression analysis provided new information about mechanisms of regu-
lation of iridoid biosynthesis.
Conclusion: Integration of data from several techniques analysed by different methods resulted in iden-
tifying key genes involved in the regulation of metabolic flux through the iridoid biosynthetic pathway
and offered an explanation why some Nepeta taxa produce only IGs, while others produce both IAs and
IGs. The obtained results pointed to new gene targets for improving iridoid production through biotech-
nology-based approaches.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6560
ER  - 

@misc{
author = "Mišić, Danijela and Gašić, Uroš and Šiler, Branislav and Dmitrović, Slavica and Skorić, Marijana and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Lukić, Tamara and Todorović, Miloš",
year = "2023",
abstract = "Introduction: Recent intensive research on the genus Nepeta has resulted in increasingly accumulated
information regarding the biosynthetic pathways and chemical evolution of iridoids; however, several in-
triguing aspects remain yet to be resolved. Our objective was to deeply investigate the molecular back-
ground of the diversity of iridoid compounds within the genus Nepeta, as well as regulative mechanisms
of their biosynthesis.
Methods: Leaves of greenhouse-grown plants were analysed for the composition of iridoid aglycones
(IAs) and iridoid glucosides (IGs) adopting non-targeted (UHPLC/LTQ Orbitrap MS n
, GC/MS) and targeted
(UHPLC/DAD/(±)HESI-MS 2 ) metabolomics approaches. Following RNA-Seq, transcriptomes of phylodi-
verse Nepeta taxa were searched for the presence/absence of iridoid-related biosynthetic genes. Co-ex-
pression patterns (qPCR) of biosynthetic genes and transcription factors were determined following
plants’ exposure to various environmental stresses.
Results: The comparison of metabolite composition among phylodiverse Nepeta taxa provided a sys-
tematic understanding of qualitative and quantitative composition of iridoids in leaves of the selected
Nepeta taxa. Mining of RNA-Seq data in search for iridoid biosynthesis-related genes pointed to signifi-
cant differences between taxa producing both IAs and IGs and those producing only IGs. Comparative
metabolomics and gene co-expression analysis provided new information about mechanisms of regu-
lation of iridoid biosynthesis.
Conclusion: Integration of data from several techniques analysed by different methods resulted in iden-
tifying key genes involved in the regulation of metabolic flux through the iridoid biosynthetic pathway
and offered an explanation why some Nepeta taxa produce only IGs, while others produce both IAs and
IGs. The obtained results pointed to new gene targets for improving iridoid production through biotech-
nology-based approaches.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6560"
}

Mišić, D., Gašić, U., Šiler, B., Dmitrović, S., Skorić, M., Matekalo, D., Nestorović Živković, J., Banjanac, T., Filipović, B., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Lukić, T.,& Todorović, M.. (2023). Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae). in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 133.
https://hdl.handle.net/21.15107/rcub_ibiss_6560

Mišić D, Gašić U, Šiler B, Dmitrović S, Skorić M, Matekalo D, Nestorović Živković J, Banjanac T, Filipović B, Milutinović M, Božunović J, Aničić N, Petrović L, Lukić T, Todorović M. Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae). in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:133.
https://hdl.handle.net/21.15107/rcub_ibiss_6560 .

Mišić, Danijela, Gašić, Uroš, Šiler, Branislav, Dmitrović, Slavica, Skorić, Marijana, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Lukić, Tamara, Todorović, Miloš, "Integration of metabolomics and transcriptomics data reveals the molecular background of the iridoid diversity within the genus Nepeta (fam. Lamiaceae)" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):133,
https://hdl.handle.net/21.15107/rcub_ibiss_6560 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6083
AB  - Data on the distribution and diversity of iridoid glycosides (IGs) and iridoid aglycones (IAs) across the genus Nepeta (fam. Lamiaceae) are fragmentary; only rarely these two subgroups of iridoids are simultaneously analysed. Our ambition was to fulfil these knowledge gaps by adopting state-of-the-art metabolomics techniques (untargeted LC-HRMS and GC/MS) to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phyllo-diverse Nepeta taxa, and thus facilitate further reconstruction of the molecular background of this diversity. Analyzed taxa were robustly grouped into three major chemotypes: (A) producers of both IGs and IAs; (B) taxa exclusively producing IGs; and (C) iridoid-lacking taxa. Furthermore, Nepeta species belonging to the chemotype A displayed significant heterogeneity in their IAs composition, and were roughly classified into several sub-chemotypes based on the stereochemistry of the major nepetalactone. No clear regularities between chemotype affiliation and phylogenetic relatedness, reconstructed adopting DNA barcoding tools, was revealed. Transcriptomes of leaves of distinguished Nepeta chemotypes were mined for the transcripts of iridoid-related biosynthetic genes, to facilitate the assessment of the factors responsible for the presence/absence of iridoids, or specific subgroups of iridoids, in these plants. Integration of metabolomics and transcriptomics data pointed to several key genes in the biosynthetic pathway as significant determinants of the iridoid diversity within the genus: geraniol synthase (GES), iridoid synthase (ISY), NAD-dependent nepetalactol-related short-chain-dehydrogenases/reductases (NEPSs), and latex protein-like genes (MLPLs). This study provided a framework to revisit some of the iridoid-metabolism-related ecological and chemical evolution theories, which is the course of our further work.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)
SP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6083
ER  - 

@conference{
author = "Mišić, Danijela and Aničić, Neda and Matekalo, Dragana and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "Data on the distribution and diversity of iridoid glycosides (IGs) and iridoid aglycones (IAs) across the genus Nepeta (fam. Lamiaceae) are fragmentary; only rarely these two subgroups of iridoids are simultaneously analysed. Our ambition was to fulfil these knowledge gaps by adopting state-of-the-art metabolomics techniques (untargeted LC-HRMS and GC/MS) to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phyllo-diverse Nepeta taxa, and thus facilitate further reconstruction of the molecular background of this diversity. Analyzed taxa were robustly grouped into three major chemotypes: (A) producers of both IGs and IAs; (B) taxa exclusively producing IGs; and (C) iridoid-lacking taxa. Furthermore, Nepeta species belonging to the chemotype A displayed significant heterogeneity in their IAs composition, and were roughly classified into several sub-chemotypes based on the stereochemistry of the major nepetalactone. No clear regularities between chemotype affiliation and phylogenetic relatedness, reconstructed adopting DNA barcoding tools, was revealed. Transcriptomes of leaves of distinguished Nepeta chemotypes were mined for the transcripts of iridoid-related biosynthetic genes, to facilitate the assessment of the factors responsible for the presence/absence of iridoids, or specific subgroups of iridoids, in these plants. Integration of metabolomics and transcriptomics data pointed to several key genes in the biosynthetic pathway as significant determinants of the iridoid diversity within the genus: geraniol synthase (GES), iridoid synthase (ISY), NAD-dependent nepetalactol-related short-chain-dehydrogenases/reductases (NEPSs), and latex protein-like genes (MLPLs). This study provided a framework to revisit some of the iridoid-metabolism-related ecological and chemical evolution theories, which is the course of our further work.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6083"
}

Mišić, D., Aničić, N., Matekalo, D., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae). in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 100.
https://hdl.handle.net/21.15107/rcub_ibiss_6083

Mišić D, Aničić N, Matekalo D, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T. Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae). in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:100.
https://hdl.handle.net/21.15107/rcub_ibiss_6083 .

Mišić, Danijela, Aničić, Neda, Matekalo, Dragana, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Combining metabolomic and transcriptomic approaches to decipher the  diversity of iridoids within the genus Nepeta (fam. Lamiaceae)" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):100,
https://hdl.handle.net/21.15107/rcub_ibiss_6083 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6480
AB  - Aim: The chemical richness of iridoids in the genus Nepeta, the exclusive representative of the subfamily Nepetoidae (Lamiaceae) that produces iridoids, has complex roles in nature, facilitating interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavour additives, fragrances, as well as toys for cats, and their bioactivities are usually assigned to nepetalactones. Data on the distribution and diversity of iridoids across the genus Nepeta are quite fragmental and only rarely, the two subgroups, iridoid glycosides (IGs) and iridoid aglycones (IAs), are simultaneously analysed. Our aim was to develop and adopt non-targeted metabolomics methods to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phylodiverse Nepeta taxa, and thus facilitate reconstruction of this diversity at the molecular level.
Material and methods: Metabolomics data were acquired adopting UHPLC/Orbitrap MS, UHPLC/QToF MS, and GC/MS. Leaves of phylodiverse/chemodiverse Nepeta taxa were subjected to RNA-Seq. DNA barcoding analysis was performed using chloroplast markers (matK, rbcL, trnL-trnF) and nuclear ribosomal ITS marker.
Main results: Nepeta taxa were robustly grouped into three major chemotypes: (1) producers of both IGs and IAs; (2) taxa exclusively producing IGs; and (3) taxa lacking iridoids. Chemotype A clade was further roughly classified into several sub-chemotypes based on the stereochemistry of the major compound  nepetalactone. Phylogenetic relations between the analysed taxa were reconstructed adopting a selected set of DNA barcoding markers. Transcriptomes of leaves of the distinguished Nepeta chemotypes were searched for transcripts of iridoid-related biosynthetic genes to facilitate the assessment of factors responsible for the presence/absence of IAs and IGs in these plants.
Conclusion: Integration of metabolomics, transcriptomics and DNA barcoding data revealed no clear regularities between chemotype affiliation and phylogenetic relatedness in analysed Nepeta taxa and pointed to several key genes in the iridoid’s biosynthetic pathway as significant determinants of the their diversity within the genus.
PB  - Farrago
C3  - Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria
T1  - Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6480
ER  - 

@conference{
author = "Mišić, Danijela and Aničić, Neda and Matekalo, Dragana and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "Aim: The chemical richness of iridoids in the genus Nepeta, the exclusive representative of the subfamily Nepetoidae (Lamiaceae) that produces iridoids, has complex roles in nature, facilitating interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavour additives, fragrances, as well as toys for cats, and their bioactivities are usually assigned to nepetalactones. Data on the distribution and diversity of iridoids across the genus Nepeta are quite fragmental and only rarely, the two subgroups, iridoid glycosides (IGs) and iridoid aglycones (IAs), are simultaneously analysed. Our aim was to develop and adopt non-targeted metabolomics methods to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phylodiverse Nepeta taxa, and thus facilitate reconstruction of this diversity at the molecular level.
Material and methods: Metabolomics data were acquired adopting UHPLC/Orbitrap MS, UHPLC/QToF MS, and GC/MS. Leaves of phylodiverse/chemodiverse Nepeta taxa were subjected to RNA-Seq. DNA barcoding analysis was performed using chloroplast markers (matK, rbcL, trnL-trnF) and nuclear ribosomal ITS marker.
Main results: Nepeta taxa were robustly grouped into three major chemotypes: (1) producers of both IGs and IAs; (2) taxa exclusively producing IGs; and (3) taxa lacking iridoids. Chemotype A clade was further roughly classified into several sub-chemotypes based on the stereochemistry of the major compound  nepetalactone. Phylogenetic relations between the analysed taxa were reconstructed adopting a selected set of DNA barcoding markers. Transcriptomes of leaves of the distinguished Nepeta chemotypes were searched for transcripts of iridoid-related biosynthetic genes to facilitate the assessment of factors responsible for the presence/absence of IAs and IGs in these plants.
Conclusion: Integration of metabolomics, transcriptomics and DNA barcoding data revealed no clear regularities between chemotype affiliation and phylogenetic relatedness in analysed Nepeta taxa and pointed to several key genes in the iridoid’s biosynthetic pathway as significant determinants of the their diversity within the genus.",
publisher = "Farrago",
journal = "Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria",
title = "Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6480"
}

Mišić, D., Aničić, N., Matekalo, D., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae). in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria
Farrago., 77.
https://hdl.handle.net/21.15107/rcub_ibiss_6480

Mišić D, Aničić N, Matekalo D, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T. Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae). in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria. 2023;:77.
https://hdl.handle.net/21.15107/rcub_ibiss_6480 .

Mišić, Danijela, Aničić, Neda, Matekalo, Dragana, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Integration of metabolomics, transcriptomica and DNA barcoding data to describe the diversity of iridoids within the genus Nepeta (Nepetoidae, fam. Lamiaceae)" in Program and Abstracts: International Seminar of Ecology - 2023: Cutting Edge Research of Ecology; 2023 Sep 28-29; Sofia, Bulgaria (2023):77,
https://hdl.handle.net/21.15107/rcub_ibiss_6480 .

TY  - CONF
AU  - Šiler, Branislav
AU  - Petrović, Luka
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Božunović, Jelena
AU  - Filipović, Biljana
AU  - Todorović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6443
AB  - Numerous species belonging to the genus Nepeta are appreciated producers of various
phenolic and iridoid compounds that have tremendously vast applicative value in
pharmacology and agriculture but are also highly esteemed as chemotaxonomical markers.
Nepeta nuda is a widespread Eurasian species, found in forest edges and clearings. We have
collected leaf samples from 12 populations across the central Balkan Peninsula and
investigated their genetic and phytochemical variations. We used 3 genomic and 7 transcriptome-derived microsatellite markers and revealed relatively low variation within the
populations. Both qualitative metabolite profiling of methanol extracts (Q-TOFF LC/MS) and
essential oils (GC/MS) analysis pointed out to high inter- and intra-population variations,
especially regarding the content of iridoids. A UHPLC-QQQ-MS/MS approach targeted towards
quantification of the most abundant iridoids and phenolics was highly efficient in the
diversification of N. nuda populations.
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans
SP  - 186
EP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6443
ER  - 

@conference{
author = "Šiler, Branislav and Petrović, Luka and Banjanac, Tijana and Lukić, Tamara and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Milutinović, Milica and Aničić, Neda and Božunović, Jelena and Filipović, Biljana and Todorović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "Numerous species belonging to the genus Nepeta are appreciated producers of various
phenolic and iridoid compounds that have tremendously vast applicative value in
pharmacology and agriculture but are also highly esteemed as chemotaxonomical markers.
Nepeta nuda is a widespread Eurasian species, found in forest edges and clearings. We have
collected leaf samples from 12 populations across the central Balkan Peninsula and
investigated their genetic and phytochemical variations. We used 3 genomic and 7 transcriptome-derived microsatellite markers and revealed relatively low variation within the
populations. Both qualitative metabolite profiling of methanol extracts (Q-TOFF LC/MS) and
essential oils (GC/MS) analysis pointed out to high inter- and intra-population variations,
especially regarding the content of iridoids. A UHPLC-QQQ-MS/MS approach targeted towards
quantification of the most abundant iridoids and phenolics was highly efficient in the
diversification of N. nuda populations.",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans",
pages = "186-187",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6443"
}

Šiler, B., Petrović, L., Banjanac, T., Lukić, T., Matekalo, D., Skorić, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Milutinović, M., Aničić, N., Božunović, J., Filipović, B., Todorović, M.,& Mišić, D.. (2023). Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 186-187.
https://hdl.handle.net/21.15107/rcub_ibiss_6443

Šiler B, Petrović L, Banjanac T, Lukić T, Matekalo D, Skorić M, Gašić U, Nestorović Živković J, Dmitrović S, Milutinović M, Aničić N, Božunović J, Filipović B, Todorović M, Mišić D. Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:186-187.
https://hdl.handle.net/21.15107/rcub_ibiss_6443 .

Šiler, Branislav, Petrović, Luka, Banjanac, Tijana, Lukić, Tamara, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Milutinović, Milica, Aničić, Neda, Božunović, Jelena, Filipović, Biljana, Todorović, Miloš, Mišić, Danijela, "Patterns of genetic and metabolic variations of Nepeta nuda L. in the central Balkans" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):186-187,
https://hdl.handle.net/21.15107/rcub_ibiss_6443 .

TY  - GEN
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Dmitrović, Slavica
AU  - Petrović, Luka
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Anđelković, Boban
AU  - Dimitrijević, Milena
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6196
AB  - Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.
PB  - Belgrade: Faculty of Chemistry
T2  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6196
ER  - 

@misc{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Božunović, Jelena and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Dmitrović, Slavica and Petrović, Luka and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Anđelković, Boban and Dimitrijević, Milena and Mišić, Danijela",
year = "2023",
abstract = "Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6196"
}

Aničić, N., Matekalo, D., Skorić, M., Božunović, J., Nestorović Živković, J., Gašić, U., Milutinović, M., Dmitrović, S., Petrović, L., Filipović, B., Banjanac, T., Šiler, B., Anđelković, B., Dimitrijević, M.,& Mišić, D.. (2023). Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry..
https://hdl.handle.net/21.15107/rcub_ibiss_6196

Aničić N, Matekalo D, Skorić M, Božunović J, Nestorović Živković J, Gašić U, Milutinović M, Dmitrović S, Petrović L, Filipović B, Banjanac T, Šiler B, Anđelković B, Dimitrijević M, Mišić D. Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:null-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Božunović, Jelena, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Dmitrović, Slavica, Petrović, Luka, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Anđelković, Boban, Dimitrijević, Milena, Mišić, Danijela, "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

TY  - CONF
AU  - Matekalo, Dragana
AU  - Aničić, Neda
AU  - Gašić, Uroš
AU  - Skorić, Marijana
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6082
AB  - The genus Nepeta is characterized by a fascinating diversity of iridoid compounds. Some species within the genus are characterized by the presence of both iridoid glycosides (IGs) and iridoid aglycones (IAs), while others produce exclusively IGs, or completely lack iridoids. The absence of certain iridoids does not necessarily imply the absence of gene transcripts dedicated to the particular steps of their biosynthetic pathway. Studying the biosynthetic pathways of iridoids in chemodiverse Nepeta species, while taking into account tissue-specific profiles of metabolites and relevant genes expression, may give us further insight into the mechanisms of regulation of the biosynthesis of these compounds. In this work we performed a comparative transcriptomic analysis of glandular trichomes and abraded leaves of Nepeta rtanjensis, an IAs- and IGs –rich species, and of IGs-producing N. grandiflora. The results of this analysis provided us with very informative data that can be used for parallel study of iridoid-related biosynthetic genes (BGs) and transcription factors (TFs) of the two chemodiverse taxa. On the other hand, differentially expressed genes in the investigated tissues, enabled us to generate a list of candidate genes for yet unelucidated steps of the iridoid metabolic pathway. These results combined with state-of-the-art metabolomics data bring us closer to understanding the molecular background of iridoid diversity within the genus Nepeta, and point to the factors involved in the regulation of the two distinct branches of the iridoid biosynthetic pathway, leading to either IGs or IAs.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6082
ER  - 

@conference{
author = "Matekalo, Dragana and Aničić, Neda and Gašić, Uroš and Skorić, Marijana and Šiler, Branislav and Banjanac, Tijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Petrović, Luka and Todorović, Miloš and Lukić, Tamara and Mišić, Danijela",
year = "2023",
abstract = "The genus Nepeta is characterized by a fascinating diversity of iridoid compounds. Some species within the genus are characterized by the presence of both iridoid glycosides (IGs) and iridoid aglycones (IAs), while others produce exclusively IGs, or completely lack iridoids. The absence of certain iridoids does not necessarily imply the absence of gene transcripts dedicated to the particular steps of their biosynthetic pathway. Studying the biosynthetic pathways of iridoids in chemodiverse Nepeta species, while taking into account tissue-specific profiles of metabolites and relevant genes expression, may give us further insight into the mechanisms of regulation of the biosynthesis of these compounds. In this work we performed a comparative transcriptomic analysis of glandular trichomes and abraded leaves of Nepeta rtanjensis, an IAs- and IGs –rich species, and of IGs-producing N. grandiflora. The results of this analysis provided us with very informative data that can be used for parallel study of iridoid-related biosynthetic genes (BGs) and transcription factors (TFs) of the two chemodiverse taxa. On the other hand, differentially expressed genes in the investigated tissues, enabled us to generate a list of candidate genes for yet unelucidated steps of the iridoid metabolic pathway. These results combined with state-of-the-art metabolomics data bring us closer to understanding the molecular background of iridoid diversity within the genus Nepeta, and point to the factors involved in the regulation of the two distinct branches of the iridoid biosynthetic pathway, leading to either IGs or IAs.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6082"
}

Matekalo, D., Aničić, N., Gašić, U., Skorić, M., Šiler, B., Banjanac, T., Nestorović Živković, J., Dmitrović, S., Filipović, B., Milutinović, M., Božunović, J., Petrović, L., Todorović, M., Lukić, T.,& Mišić, D.. (2023). Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 82.
https://hdl.handle.net/21.15107/rcub_ibiss_6082

Matekalo D, Aničić N, Gašić U, Skorić M, Šiler B, Banjanac T, Nestorović Živković J, Dmitrović S, Filipović B, Milutinović M, Božunović J, Petrović L, Todorović M, Lukić T, Mišić D. Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:82.
https://hdl.handle.net/21.15107/rcub_ibiss_6082 .

Matekalo, Dragana, Aničić, Neda, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela, "Uncovering the tissue-specific regulation of iridoid biosynthesis in two  chemodiverse Nepeta species: integration of transcriptomics and metabolomics data" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):82,
https://hdl.handle.net/21.15107/rcub_ibiss_6082 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Gašić, Uroš
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6081
AB  - Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Functional iridoid synthases from three Nepeta species with differing iridoid  profiles
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6081
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Gašić, Uroš and Dimitrijević, Milena and Anđelković, Boban and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Mišić, Danijela",
year = "2023",
abstract = "Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6081"
}

Aničić, N., Matekalo, D., Skorić, M., Nestorović Živković, J., Dmitrović, S., Gašić, U., Dimitrijević, M., Anđelković, B., Božunović, J., Milutinović, M., Petrović, L.,& Mišić, D.. (2023). Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081

Aničić N, Matekalo D, Skorić M, Nestorović Živković J, Dmitrović S, Gašić U, Dimitrijević M, Anđelković B, Božunović J, Milutinović M, Petrović L, Mišić D. Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Gašić, Uroš, Dimitrijević, Milena, Anđelković, Boban, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Mišić, Danijela, "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):80,
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

TY  - CONF
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Šiler, Branislav
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Lukić, Tamara
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5561
AB  - The genus Nepeta, one of the largest genera of the Lamiaceae family (subfamily Nepetoidae, tribe Nepeteae) comprises around 250 herbaceous, perennial, rarely annual species, native to temperate Europe, Asia, North Africa, and the mountainous region of tropical Africa. Phytochemical investigations have revealed monoterpenes (iridoids), diterpenes, triterpenes, as well as phenolics, including flavonoids, to be the major specialized metabolites in these remarkable plants. The chemical richness of terpenoids and phenolics in catmints plays functional roles in nature, facilitating complex interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavor additives, fragrances, and toys for cats, and their extensively studied bioactivities are mainly assigned to iridoid monoterpenoids nepetalactones. These compounds are proven repellents against insects (e.g. mosquitos, cockroaches, ticks, flies, mites, termites), and promising bioherbicides against a vast array of weeds (e.g. amaranth, ragweed, goosefoot, dandelion).
In spite of the plenitude of fundamental research that has documented the vast bioactive potential of Nepeta species, their commercial exploitation is restrained. There are several important factors that make the commercialization of Nepeta sp. most challenging, including limited bioresources, limited availability of bioactive compounds in purified forms, challenges to optimize the cultivation and standardize the quality of plant material, low number of studies focusing on the effects on non-target organisms and on the mutual relationships of individual compounds, instability and relatively low retainability of volatile nepetalactones, and others.
The challenge ahead is to provide sustainable sources of bioactive compounds of Nepeta sp. and thus establish a background for their commercialization. One of the solutions might be the creation of alternative sources of these biomolecules through synthetic biology approaches, thereby developing microbial or plant systems producing bioactive compounds of interest. Essential prerequisites to achieve this is the comprehensive and simultaneous acquiring, analyzing and interpreting the overall diversity of specialized metabolites within the genus Nepeta, reconstruction of the molecular background of the chemical diversity and elucidation of the biosynthetic pathways, as well as adopting the biotechnology tools to scale-up the production of the compounds of interest.
PB  - Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences
C3  - Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria
T1  - Catmints (Nepeta sp.): Biological activities and applicative potential
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5561
ER  - 

@conference{
author = "Mišić, Danijela and Gašić, Uroš and Šiler, Branislav and Dmitrović, Slavica and Skorić, Marijana and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Filipović, Biljana and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Todorović, Miloš and Lukić, Tamara",
year = "2023",
abstract = "The genus Nepeta, one of the largest genera of the Lamiaceae family (subfamily Nepetoidae, tribe Nepeteae) comprises around 250 herbaceous, perennial, rarely annual species, native to temperate Europe, Asia, North Africa, and the mountainous region of tropical Africa. Phytochemical investigations have revealed monoterpenes (iridoids), diterpenes, triterpenes, as well as phenolics, including flavonoids, to be the major specialized metabolites in these remarkable plants. The chemical richness of terpenoids and phenolics in catmints plays functional roles in nature, facilitating complex interactions with insects, phytopathogens, and other co-occurring plants. On the other hand, Nepeta species are used widely by humans as medicines, flavor additives, fragrances, and toys for cats, and their extensively studied bioactivities are mainly assigned to iridoid monoterpenoids nepetalactones. These compounds are proven repellents against insects (e.g. mosquitos, cockroaches, ticks, flies, mites, termites), and promising bioherbicides against a vast array of weeds (e.g. amaranth, ragweed, goosefoot, dandelion).
In spite of the plenitude of fundamental research that has documented the vast bioactive potential of Nepeta species, their commercial exploitation is restrained. There are several important factors that make the commercialization of Nepeta sp. most challenging, including limited bioresources, limited availability of bioactive compounds in purified forms, challenges to optimize the cultivation and standardize the quality of plant material, low number of studies focusing on the effects on non-target organisms and on the mutual relationships of individual compounds, instability and relatively low retainability of volatile nepetalactones, and others.
The challenge ahead is to provide sustainable sources of bioactive compounds of Nepeta sp. and thus establish a background for their commercialization. One of the solutions might be the creation of alternative sources of these biomolecules through synthetic biology approaches, thereby developing microbial or plant systems producing bioactive compounds of interest. Essential prerequisites to achieve this is the comprehensive and simultaneous acquiring, analyzing and interpreting the overall diversity of specialized metabolites within the genus Nepeta, reconstruction of the molecular background of the chemical diversity and elucidation of the biosynthetic pathways, as well as adopting the biotechnology tools to scale-up the production of the compounds of interest.",
publisher = "Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences",
journal = "Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria",
title = "Catmints (Nepeta sp.): Biological activities and applicative potential",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5561"
}

Mišić, D., Gašić, U., Šiler, B., Dmitrović, S., Skorić, M., Matekalo, D., Nestorović Živković, J., Banjanac, T., Filipović, B., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Todorović, M.,& Lukić, T.. (2023). Catmints (Nepeta sp.): Biological activities and applicative potential. in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria
Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_5561

Mišić D, Gašić U, Šiler B, Dmitrović S, Skorić M, Matekalo D, Nestorović Živković J, Banjanac T, Filipović B, Milutinović M, Božunović J, Aničić N, Petrović L, Todorović M, Lukić T. Catmints (Nepeta sp.): Biological activities and applicative potential. in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_5561 .

Mišić, Danijela, Gašić, Uroš, Šiler, Branislav, Dmitrović, Slavica, Skorić, Marijana, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, "Catmints (Nepeta sp.): Biological activities and applicative potential" in Program and Book of Abstracts: Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products - ICSUMAP’23; 2023 March 27-28; Sofia, Bulgaria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_5561 .

TY  - CONF
AU  - Rajaković, Mladen
AU  - Gašić, Uroš
AU  - Bukvički, Danka
AU  - Pešić, Mirjana
AU  - Ćirić, Ana
AU  - Milinčić, Danijel
AU  - Stojković, Dejan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6437
AB  - According to recent research, a variety of inedible portions of fruits and vegetables, as well as food waste, are excellent sources of phytochemicals that may be isolated and reintroduced as natural food additives into the food chain [1]. Besides the food industry, natural product from non-edible parts of the fruits of Cucurbitaceae family can be used in other industries such as cosmetic and pharmaceutical and the fact that the plants in this family are also used medicinally makes them excellent research subjects for the study of plants with high therapeutic potential [2]. The focus of our study was the solid/liquid extraction of lyophilized fruit peels of Cucumis metuliferus E. Mey. (Cucurbitaceae) under different extraction conditions such as extraction time (min), ethanol/water ratio (%) and power of ultrasonic bath (%). It was aimed as well to determine the phytochemical composition of 25 different ethanolic and hydro-ethanolic extracts by performing UHPLC-QToF-MS analysis. The Figure 1 shows the fruit of Horned Melon (C. metuliferus) and the exocarp as part of fruit used for extraction. Under different extraction conditions percentage yields of the dry extracts varied between 26.37% - 43.98%, obtaining the optimal extraction conditions as follows: EtOH (%): 50; Amplitude (%): 40; Time (min): 30. Furthermore, chemical profiling of the extracts revealed the presence of the following compounds: p-hydroxybenzoic acid, dihydroxybenzoic acid, galloyl pentoside, dihydroxybenzoyl pentoside, hydroxybenzoyl hexoside, vanilloyl hexoside and hydroxybenzoyl rhamnosyl hexoside. This is the first study that highlights the optimization of extraction conditions of the C. metuliferus peels, as well as chemical identification of bioactive compounds present in the extracts. Our future observations will be oriented towards exploring bioactive properties of the obtained extracts and further incorporations in food matrices.
PB  - Belgrade: Serbian Chemical Society
C3  - Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia
T1  - Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels
SP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6437
ER  - 

@conference{
author = "Rajaković, Mladen and Gašić, Uroš and Bukvički, Danka and Pešić, Mirjana and Ćirić, Ana and Milinčić, Danijel and Stojković, Dejan",
year = "2023",
abstract = "According to recent research, a variety of inedible portions of fruits and vegetables, as well as food waste, are excellent sources of phytochemicals that may be isolated and reintroduced as natural food additives into the food chain [1]. Besides the food industry, natural product from non-edible parts of the fruits of Cucurbitaceae family can be used in other industries such as cosmetic and pharmaceutical and the fact that the plants in this family are also used medicinally makes them excellent research subjects for the study of plants with high therapeutic potential [2]. The focus of our study was the solid/liquid extraction of lyophilized fruit peels of Cucumis metuliferus E. Mey. (Cucurbitaceae) under different extraction conditions such as extraction time (min), ethanol/water ratio (%) and power of ultrasonic bath (%). It was aimed as well to determine the phytochemical composition of 25 different ethanolic and hydro-ethanolic extracts by performing UHPLC-QToF-MS analysis. The Figure 1 shows the fruit of Horned Melon (C. metuliferus) and the exocarp as part of fruit used for extraction. Under different extraction conditions percentage yields of the dry extracts varied between 26.37% - 43.98%, obtaining the optimal extraction conditions as follows: EtOH (%): 50; Amplitude (%): 40; Time (min): 30. Furthermore, chemical profiling of the extracts revealed the presence of the following compounds: p-hydroxybenzoic acid, dihydroxybenzoic acid, galloyl pentoside, dihydroxybenzoyl pentoside, hydroxybenzoyl hexoside, vanilloyl hexoside and hydroxybenzoyl rhamnosyl hexoside. This is the first study that highlights the optimization of extraction conditions of the C. metuliferus peels, as well as chemical identification of bioactive compounds present in the extracts. Our future observations will be oriented towards exploring bioactive properties of the obtained extracts and further incorporations in food matrices.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia",
title = "Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels",
pages = "171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6437"
}

Rajaković, M., Gašić, U., Bukvički, D., Pešić, M., Ćirić, A., Milinčić, D.,& Stojković, D.. (2023). Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels. in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia
Belgrade: Serbian Chemical Society., 171.
https://hdl.handle.net/21.15107/rcub_ibiss_6437

Rajaković M, Gašić U, Bukvički D, Pešić M, Ćirić A, Milinčić D, Stojković D. Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels. in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia. 2023;:171.
https://hdl.handle.net/21.15107/rcub_ibiss_6437 .

Rajaković, Mladen, Gašić, Uroš, Bukvički, Danka, Pešić, Mirjana, Ćirić, Ana, Milinčić, Danijel, Stojković, Dejan, "Phytochemical composition of hydro-ethanolic extracts from  Cucumis metuliferus E. Mey. fruit peels" in Abstract book: 22nd EuroFoodChem Congress; 2023 Jun 14-16; Belgrade, Serbia (2023):171,
https://hdl.handle.net/21.15107/rcub_ibiss_6437 .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6456
AB  - Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.
PB  - Lausanne, Switzerland: Frontiers
T2  - Frontiers in Plant Science
T1  - Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)
VL  - 14
DO  - 10.3389/fpls.2023.1211453
SP  - 1211453
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Dimitrijević, Milena and Anđelković, Boban and Mišić, Danijela",
year = "2023",
abstract = "Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.",
publisher = "Lausanne, Switzerland: Frontiers",
journal = "Frontiers in Plant Science",
title = "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)",
volume = "14",
doi = "10.3389/fpls.2023.1211453",
pages = "1211453"
}

Aničić, N., Matekalo, D., Skorić, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Božunović, J., Milutinović, M., Petrović, L., Dimitrijević, M., Anđelković, B.,& Mišić, D.. (2023). Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science
Lausanne, Switzerland: Frontiers., 14, 1211453.
https://doi.org/10.3389/fpls.2023.1211453

Aničić N, Matekalo D, Skorić M, Gašić U, Nestorović Živković J, Dmitrović S, Božunović J, Milutinović M, Petrović L, Dimitrijević M, Anđelković B, Mišić D. Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science. 2023;14:1211453.
doi:10.3389/fpls.2023.1211453 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Dimitrijević, Milena, Anđelković, Boban, Mišić, Danijela, "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)" in Frontiers in Plant Science, 14 (2023):1211453,
https://doi.org/10.3389/fpls.2023.1211453 . .

TY  - JOUR
AU  - Mattoo, Autar
AU  - Cavigelli, Michel A.
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Maksimović, Vuk
AU  - Kramer, Matthew
AU  - Kaur, Bhavneet
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Roberts, Daniel
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6255
AB  - Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.
PB  - Frontiers
T2  - Frontiers in Sustainable Food Systems
T1  - Maize metabolomics in relation to cropping system and growing year
VL  - 7
DO  - 10.3389/fsufs.2023.1130089
SP  - 1130089
ER  - 

@article{
author = "Mattoo, Autar and Cavigelli, Michel A. and Mišić, Danijela and Gašić, Uroš and Maksimović, Vuk and Kramer, Matthew and Kaur, Bhavneet and Matekalo, Dragana and Nestorović Živković, Jasmina and Roberts, Daniel",
year = "2023",
abstract = "Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.",
publisher = "Frontiers",
journal = "Frontiers in Sustainable Food Systems",
title = "Maize metabolomics in relation to cropping system and growing year",
volume = "7",
doi = "10.3389/fsufs.2023.1130089",
pages = "1130089"
}

Mattoo, A., Cavigelli, M. A., Mišić, D., Gašić, U., Maksimović, V., Kramer, M., Kaur, B., Matekalo, D., Nestorović Živković, J.,& Roberts, D.. (2023). Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems
Frontiers., 7, 1130089.
https://doi.org/10.3389/fsufs.2023.1130089

Mattoo A, Cavigelli MA, Mišić D, Gašić U, Maksimović V, Kramer M, Kaur B, Matekalo D, Nestorović Živković J, Roberts D. Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems. 2023;7:1130089.
doi:10.3389/fsufs.2023.1130089 .

Mattoo, Autar, Cavigelli, Michel A., Mišić, Danijela, Gašić, Uroš, Maksimović, Vuk, Kramer, Matthew, Kaur, Bhavneet, Matekalo, Dragana, Nestorović Živković, Jasmina, Roberts, Daniel, "Maize metabolomics in relation to cropping system and growing year" in Frontiers in Sustainable Food Systems, 7 (2023):1130089,
https://doi.org/10.3389/fsufs.2023.1130089 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6466
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
IS  - 7
VL  - 12
DO  - 10.3390/antiox12071424
SP  - 1424
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš and Tešić, Živoslav and Stanojević, Sladjana and Pešić, Mirjana",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
number = "7",
volume = "12",
doi = "10.3390/antiox12071424",
pages = "1424"
}

Kostić, A., Milinčić, D., Špirović Trifunović, B., Nedić, N., Gašić, U., Tešić, Ž., Stanojević, S.,& Pešić, M.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants
Basel: MDPI., 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić A, Milinčić D, Špirović Trifunović B, Nedić N, Gašić U, Tešić Ž, Stanojević S, Pešić M. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar, Milinčić, Danijel, Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš, Tešić, Živoslav, Stanojević, Sladjana, Pešić, Mirjana, "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6463
AB  - Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.
PB  - Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
T1  - Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6463
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Petrović, Jovana and Stojković, Dejan and Gašić, Uroš and Milinčić, Danijel and Pešić, Mirjana",
year = "2023",
abstract = "Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.",
publisher = "Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia",
title = "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6463"
}

Ćirić, A., Ivanov, M., Petrović, J., Stojković, D., Gašić, U., Milinčić, D.,& Pešić, M.. (2023). Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463

Ćirić A, Ivanov M, Petrović J, Stojković D, Gašić U, Milinčić D, Pešić M. Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia. 2023;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

Ćirić, Ana, Ivanov, Marija, Petrović, Jovana, Stojković, Dejan, Gašić, Uroš, Milinčić, Danijel, Pešić, Mirjana, "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications" in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia (2023):41,
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

TY  - JOUR
AU  - Brkljača, Nemanja
AU  - Đurović, Saša
AU  - Milošević, Sanja
AU  - Gašić, Uroš
AU  - Panković, Dejana
AU  - Zeković, Zoran
AU  - Pavlić, Branimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6469
AB  - Cannabis sativa L. known as industrial hemp manifests high capability in the manufacturing of valuable nutraceuticals with health benefits. This study was focused on investigation of chemical profile and bioactive potential of extracts isolated from three hemp varieties (Helena, Marina and Fedora 17). In addition, great attention was directed towards the use of green extraction techniques and maximum utilization of raw material. In that regard conventional (hydrodistillation – HD) and novel extraction techniques (microwave-assisted hydrodistillation – MWHD, supercritical fluid extraction – SFE and microwave-assisted extraction – MAE) were applied for isolating various fractions (essential oil, lipid extracts and liquid extract) with promising bioactive potential. Essential oils and lipid extracts were characterized regarding yield, terpenoid profile determined by GC-MS and in vitro antioxidant activity towards DPPH and ABTS + radicals. Furthermore, the content of two major cannabinoids was determined in plant material and in extract obtained by SFE. Sequential SFE technique allows obtaining lipid extracts in the first step of process as well as raffinates after SFE in the second step. MAE was applied for isolation of polyphenolic fraction from raw materials and SFE raffinates. Polyphenolic content was determined by spectrophotometric assays and UHPLC-Triple-quadrupole-MS. The results suggested that hemp is an excellent source of valuable terpenoid, cannabinoid and polyphenolic antioxidant agents, and further research should be focused on their utilization in dietary supplements and functional foods.
PB  - Elsevier
T2  - Sustainable Chemistry and Pharmacy
T1  - Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds
VL  - 35
DO  - 10.1016/j.scp.2023.101213
SP  - 101213
ER  - 

@article{
author = "Brkljača, Nemanja and Đurović, Saša and Milošević, Sanja and Gašić, Uroš and Panković, Dejana and Zeković, Zoran and Pavlić, Branimir",
year = "2023",
abstract = "Cannabis sativa L. known as industrial hemp manifests high capability in the manufacturing of valuable nutraceuticals with health benefits. This study was focused on investigation of chemical profile and bioactive potential of extracts isolated from three hemp varieties (Helena, Marina and Fedora 17). In addition, great attention was directed towards the use of green extraction techniques and maximum utilization of raw material. In that regard conventional (hydrodistillation – HD) and novel extraction techniques (microwave-assisted hydrodistillation – MWHD, supercritical fluid extraction – SFE and microwave-assisted extraction – MAE) were applied for isolating various fractions (essential oil, lipid extracts and liquid extract) with promising bioactive potential. Essential oils and lipid extracts were characterized regarding yield, terpenoid profile determined by GC-MS and in vitro antioxidant activity towards DPPH and ABTS + radicals. Furthermore, the content of two major cannabinoids was determined in plant material and in extract obtained by SFE. Sequential SFE technique allows obtaining lipid extracts in the first step of process as well as raffinates after SFE in the second step. MAE was applied for isolation of polyphenolic fraction from raw materials and SFE raffinates. Polyphenolic content was determined by spectrophotometric assays and UHPLC-Triple-quadrupole-MS. The results suggested that hemp is an excellent source of valuable terpenoid, cannabinoid and polyphenolic antioxidant agents, and further research should be focused on their utilization in dietary supplements and functional foods.",
publisher = "Elsevier",
journal = "Sustainable Chemistry and Pharmacy",
title = "Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds",
volume = "35",
doi = "10.1016/j.scp.2023.101213",
pages = "101213"
}

Brkljača, N., Đurović, S., Milošević, S., Gašić, U., Panković, D., Zeković, Z.,& Pavlić, B.. (2023). Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds. in Sustainable Chemistry and Pharmacy
Elsevier., 35, 101213.
https://doi.org/10.1016/j.scp.2023.101213

Brkljača N, Đurović S, Milošević S, Gašić U, Panković D, Zeković Z, Pavlić B. Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds. in Sustainable Chemistry and Pharmacy. 2023;35:101213.
doi:10.1016/j.scp.2023.101213 .

Brkljača, Nemanja, Đurović, Saša, Milošević, Sanja, Gašić, Uroš, Panković, Dejana, Zeković, Zoran, Pavlić, Branimir, "Sequential extraction approach for sustainable recovery of various hemp (Cannabis sativa L.) bioactive compounds" in Sustainable Chemistry and Pharmacy, 35 (2023):101213,
https://doi.org/10.1016/j.scp.2023.101213 . .

TY  - JOUR
AU  - Cvetanović Kljakić, Aleksandra
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Seebaluck-Sandoram, Roumita
AU  - Ibrahim Uba, Abdullahi
AU  - Fawzi Mahomoodally, Mohamad
AU  - Yildiztugay, Evren
AU  - Zengin, Gokhan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6470
AB  - Glaucosciadium cordifolium (Apiaceae) is traditionally used against stomach ailments, as aphrodisiac and appetizer. This study endeavored to characterize several extracts of G. cordifolium aerial parts (prepared by various isolation procedures (ASE: Accelerated solvent extraction; HAE: homogenizer-assisted extract; MAE: Microwave-assisted extraction; MAC: Maceration; SFE: supercritical CO2 extraction; SOX: Soxhlet; UAE: Ultrasound-assisted extraction) and evaluate the antioxidant activity and inhibitory activity against clinical enzymes. In order to describe the composition of the extracts liquid chromatography-mass spectrometry (LC-MS) analysis was employed. Of the total number of confirmed polyphenol components (15 in total), four were dominant in their content: 5-O-caffeoylquinic acid, p-coumaric acid, quercetin-3-O-glucoside and quercetin-3-O-rhamnoside. Spectrophotometric analysis showed that the total phenolic and flavonoid contents differed among the extracts, which was influenced by extraction technique used for their preparation. The phenolic content of the different extracts varied in the order HAE > UAE > MAC > SOX > MAE > ASE > SFE. HAE extract yielded the highest phenolic content (49.97 mg gallic acid equivalent (GAE)/g) while SFE demonstrated the lowest phenolic content (21.65 mg GAE/g). MAC extracts contained the highest flavonoids (50.35 mg rutin equivalent (RE)/g) while SFE extract showed the lowest flavonoid content (10.87 mg RE/g). UAE, MAC, MAE, HAE, SOX and ASE showed significant DPPH antioxidant activities and the values of radical inhibitory capacity varied in the range of 55.79–54.99 mg TE/g. SFE demonstrated weak DPPH (1,1-diphenyl-2-picrylhydrazyl) radical inhibitory activity with a value 7.0 mg TE/g. UAE showed the highest ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) cation radical inhibitory potential (122.41 mg trolox equivalent (TE)/g). The CUPRAC (cupric reducing antioxidant capacity) activity of UAE was remarkable with a value of 193.58 mg TE/g. A gradual decrease in activity was observed with extracts obtained from other extraction techniques with the lowest antioxidant activity (77.0 mg TE/g) obtained from SFE extract. The highest antioxidant recorded by Phosphomolybdenum was found in HAE extract (1.62 mmol TE/g). SFE showed the highest metal chelating activity (27.30 mg ethylenediamine tetraacetic acid (EDTAE)/g). Regarding the acetylcholinesterase (AChE) inhibitory activity, MAE and ASE extracts showed the highest potential with a value of 4.06 and 4.05 mg galanthamine equivalent (GALAE)/g respectively. All tested extracts showed considerable whitening activity and SFE revealed the highest activity against tyrosinase (93.82 mg kojic acid equivalent (KAE)/g). In addition, SFE showed the best inhibitory ability against amylase (0.71 mmol acarbose equivalent (ACAE)/g). However, the extracts had similar glucosidase inhibitory effects (1.64–1.71 mmol ACAE/g). The rich chemical composition of the extracts and joint action of different classes of polyphenolic compounds in them may be responsible for their high biological activity. Extracts with the highest activity could serve in the future as an important tool for controlling the activity of clinically important enzymes and take their place in the official formulation after additional pharmacological tests.
PB  - Elsevier
T2  - Process Biochemistry
T1  - Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects
IS  - Part 1
VL  - 134
DO  - 10.1016/j.procbio.2023.10.007
SP  - 141
EP  - 150
ER  - 

@article{
author = "Cvetanović Kljakić, Aleksandra and Božunović, Jelena and Gašić, Uroš and Seebaluck-Sandoram, Roumita and Ibrahim Uba, Abdullahi and Fawzi Mahomoodally, Mohamad and Yildiztugay, Evren and Zengin, Gokhan",
year = "2023",
abstract = "Glaucosciadium cordifolium (Apiaceae) is traditionally used against stomach ailments, as aphrodisiac and appetizer. This study endeavored to characterize several extracts of G. cordifolium aerial parts (prepared by various isolation procedures (ASE: Accelerated solvent extraction; HAE: homogenizer-assisted extract; MAE: Microwave-assisted extraction; MAC: Maceration; SFE: supercritical CO2 extraction; SOX: Soxhlet; UAE: Ultrasound-assisted extraction) and evaluate the antioxidant activity and inhibitory activity against clinical enzymes. In order to describe the composition of the extracts liquid chromatography-mass spectrometry (LC-MS) analysis was employed. Of the total number of confirmed polyphenol components (15 in total), four were dominant in their content: 5-O-caffeoylquinic acid, p-coumaric acid, quercetin-3-O-glucoside and quercetin-3-O-rhamnoside. Spectrophotometric analysis showed that the total phenolic and flavonoid contents differed among the extracts, which was influenced by extraction technique used for their preparation. The phenolic content of the different extracts varied in the order HAE > UAE > MAC > SOX > MAE > ASE > SFE. HAE extract yielded the highest phenolic content (49.97 mg gallic acid equivalent (GAE)/g) while SFE demonstrated the lowest phenolic content (21.65 mg GAE/g). MAC extracts contained the highest flavonoids (50.35 mg rutin equivalent (RE)/g) while SFE extract showed the lowest flavonoid content (10.87 mg RE/g). UAE, MAC, MAE, HAE, SOX and ASE showed significant DPPH antioxidant activities and the values of radical inhibitory capacity varied in the range of 55.79–54.99 mg TE/g. SFE demonstrated weak DPPH (1,1-diphenyl-2-picrylhydrazyl) radical inhibitory activity with a value 7.0 mg TE/g. UAE showed the highest ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) cation radical inhibitory potential (122.41 mg trolox equivalent (TE)/g). The CUPRAC (cupric reducing antioxidant capacity) activity of UAE was remarkable with a value of 193.58 mg TE/g. A gradual decrease in activity was observed with extracts obtained from other extraction techniques with the lowest antioxidant activity (77.0 mg TE/g) obtained from SFE extract. The highest antioxidant recorded by Phosphomolybdenum was found in HAE extract (1.62 mmol TE/g). SFE showed the highest metal chelating activity (27.30 mg ethylenediamine tetraacetic acid (EDTAE)/g). Regarding the acetylcholinesterase (AChE) inhibitory activity, MAE and ASE extracts showed the highest potential with a value of 4.06 and 4.05 mg galanthamine equivalent (GALAE)/g respectively. All tested extracts showed considerable whitening activity and SFE revealed the highest activity against tyrosinase (93.82 mg kojic acid equivalent (KAE)/g). In addition, SFE showed the best inhibitory ability against amylase (0.71 mmol acarbose equivalent (ACAE)/g). However, the extracts had similar glucosidase inhibitory effects (1.64–1.71 mmol ACAE/g). The rich chemical composition of the extracts and joint action of different classes of polyphenolic compounds in them may be responsible for their high biological activity. Extracts with the highest activity could serve in the future as an important tool for controlling the activity of clinically important enzymes and take their place in the official formulation after additional pharmacological tests.",
publisher = "Elsevier",
journal = "Process Biochemistry",
title = "Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects",
number = "Part 1",
volume = "134",
doi = "10.1016/j.procbio.2023.10.007",
pages = "141-150"
}

Cvetanović Kljakić, A., Božunović, J., Gašić, U., Seebaluck-Sandoram, R., Ibrahim Uba, A., Fawzi Mahomoodally, M., Yildiztugay, E.,& Zengin, G.. (2023). Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects. in Process Biochemistry
Elsevier., 134(Part 1), 141-150.
https://doi.org/10.1016/j.procbio.2023.10.007

Cvetanović Kljakić A, Božunović J, Gašić U, Seebaluck-Sandoram R, Ibrahim Uba A, Fawzi Mahomoodally M, Yildiztugay E, Zengin G. Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects. in Process Biochemistry. 2023;134(Part 1):141-150.
doi:10.1016/j.procbio.2023.10.007 .

Cvetanović Kljakić, Aleksandra, Božunović, Jelena, Gašić, Uroš, Seebaluck-Sandoram, Roumita, Ibrahim Uba, Abdullahi, Fawzi Mahomoodally, Mohamad, Yildiztugay, Evren, Zengin, Gokhan, "Chemical characterization of Glaucosciadum cordifolium extracts obtained by different extraction techniques and their biopharmaceutical effects" in Process Biochemistry, 134, no. Part 1 (2023):141-150,
https://doi.org/10.1016/j.procbio.2023.10.007 . .

TY  - JOUR
AU  - Cvetanović Kljakić, Aleksandra
AU  - Stupar, Alena
AU  - Terzić, Milena
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Yildiztugay, Evren
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6468
AB  - The plant species Opopanax hispidus is known in traditional Turkish medicine and nutrition. In addition to all the advantages that are known by the traditions of old generations, O. hispidus has been insufficiently examined, and its potential could be used in treatment in modern medicine and diet. In this paper, O. hispidus extracts obtained by conventional (maceration (MAC), and Soxhlet (SOX)) and contemporary (accelerated solvent extraction (ASE), homogenizer assisted extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), supercritical fluid extraction (SFE)) methods were examined in terms of their chemical composition and biological potential. The extract obtained by HAE extraction had the highest content of total phenolics and flavonoids (36.98 mg GAE/g and 15.04 mg RE/g, respectively). The highest antioxidant activity was recorded for HAE and ASE extracts. O. hispidus extracts achieved inhibitory activity against acetylcholinesterase (4.12–6.44 mg GALAE/g), tyrosinase (44.78–76.72 mg KAE/g), α-amylase (0.35–0.46 mmol ACAE/g), and α-glucosidase (1.67–1.77 mmol ACAE/g) enzymes. LC-MS technique was used for the quantitative analysis of the extracts, allowing the quantification of chlorogenic acid, neochlorogenic acid, and p-coumaric acid, rutin, luteolin, and kaempferol 3-O-glucoside as the most abundant phenolic components. Results suggested that the green extraction techniques for obtaining extracts that are rich in bioactive compounds have advantages compared to conventional extraction. Therefore, these extracts can be used as antioxidants, neuroprotective, antityrosinase, and antidiabetic agents while respecting the principles of sustainable development and green chemistry.
PB  - Elsevier
T2  - Sustainable Chemistry and Pharmacy
T1  - Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies
VL  - 33
DO  - 10.1016/j.scp.2023.101122
SP  - 101122
ER  - 

@article{
author = "Cvetanović Kljakić, Aleksandra and Stupar, Alena and Terzić, Milena and Božunović, Jelena and Gašić, Uroš and Zengin, Gokhan and Yildiztugay, Evren",
year = "2023",
abstract = "The plant species Opopanax hispidus is known in traditional Turkish medicine and nutrition. In addition to all the advantages that are known by the traditions of old generations, O. hispidus has been insufficiently examined, and its potential could be used in treatment in modern medicine and diet. In this paper, O. hispidus extracts obtained by conventional (maceration (MAC), and Soxhlet (SOX)) and contemporary (accelerated solvent extraction (ASE), homogenizer assisted extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), supercritical fluid extraction (SFE)) methods were examined in terms of their chemical composition and biological potential. The extract obtained by HAE extraction had the highest content of total phenolics and flavonoids (36.98 mg GAE/g and 15.04 mg RE/g, respectively). The highest antioxidant activity was recorded for HAE and ASE extracts. O. hispidus extracts achieved inhibitory activity against acetylcholinesterase (4.12–6.44 mg GALAE/g), tyrosinase (44.78–76.72 mg KAE/g), α-amylase (0.35–0.46 mmol ACAE/g), and α-glucosidase (1.67–1.77 mmol ACAE/g) enzymes. LC-MS technique was used for the quantitative analysis of the extracts, allowing the quantification of chlorogenic acid, neochlorogenic acid, and p-coumaric acid, rutin, luteolin, and kaempferol 3-O-glucoside as the most abundant phenolic components. Results suggested that the green extraction techniques for obtaining extracts that are rich in bioactive compounds have advantages compared to conventional extraction. Therefore, these extracts can be used as antioxidants, neuroprotective, antityrosinase, and antidiabetic agents while respecting the principles of sustainable development and green chemistry.",
publisher = "Elsevier",
journal = "Sustainable Chemistry and Pharmacy",
title = "Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies",
volume = "33",
doi = "10.1016/j.scp.2023.101122",
pages = "101122"
}

Cvetanović Kljakić, A., Stupar, A., Terzić, M., Božunović, J., Gašić, U., Zengin, G.,& Yildiztugay, E.. (2023). Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies. in Sustainable Chemistry and Pharmacy
Elsevier., 33, 101122.
https://doi.org/10.1016/j.scp.2023.101122

Cvetanović Kljakić A, Stupar A, Terzić M, Božunović J, Gašić U, Zengin G, Yildiztugay E. Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies. in Sustainable Chemistry and Pharmacy. 2023;33:101122.
doi:10.1016/j.scp.2023.101122 .

Cvetanović Kljakić, Aleksandra, Stupar, Alena, Terzić, Milena, Božunović, Jelena, Gašić, Uroš, Zengin, Gokhan, Yildiztugay, Evren, "Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies" in Sustainable Chemistry and Pharmacy, 33 (2023):101122,
https://doi.org/10.1016/j.scp.2023.101122 . .

TY  - JOUR
AU  - Miletić, Milica
AU  - Ivanov, Marija
AU  - Topalović, Aleksandra
AU  - Gavrilović, Milan
AU  - Gašić, Uroš
AU  - Janaćković, Peđa
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6465
AB  - Xanthium spinosum is a cosmopolitan annual herb used in traditional medicine worldwide. Although known from ethnobotanical studies, the species is scarcely investigated from the aspects of phytochemistry and biological activity. Therefore, the phenolic composition and biological activity of X. spinosum were examined. Plant specialised metabolites (phenolics) extracted from the roots, leaves and fruits with dichloromethane:methanol (1:1) were analysed by liquid chromatography mass spectrometry (LC-MS). In total 10 phenolic compounds were identified and quantified. Six compounds were common to all the extracts. Chlorogenic acid was the most abundant constituent in all the extracts (4.262 mg/g in the fruit extract, 0.820 mg/g in the leaf extract, and 0.540 mg/g in the root extract). The biological activity (antimicrobial and antibiofilm) of the extracts was tested against 12 microfungi and 12 bacterial strains by the microdilution method. All the extracts exhibited moderate antimicrobial and antibiofilm activity and inhibited the growth of most of the examined microorganisms. The obtained results indicate the potential role of the tested extracts in pharmacy and medicine.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac"
T2  - Botanica Serbica
T1  - The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts
IS  - 1
VL  - 47
DO  - 10.2298/BOTSERB2301093M
SP  - 93
EP  - 101
ER  - 

@article{
author = "Miletić, Milica and Ivanov, Marija and Topalović, Aleksandra and Gavrilović, Milan and Gašić, Uroš and Janaćković, Peđa",
year = "2023",
abstract = "Xanthium spinosum is a cosmopolitan annual herb used in traditional medicine worldwide. Although known from ethnobotanical studies, the species is scarcely investigated from the aspects of phytochemistry and biological activity. Therefore, the phenolic composition and biological activity of X. spinosum were examined. Plant specialised metabolites (phenolics) extracted from the roots, leaves and fruits with dichloromethane:methanol (1:1) were analysed by liquid chromatography mass spectrometry (LC-MS). In total 10 phenolic compounds were identified and quantified. Six compounds were common to all the extracts. Chlorogenic acid was the most abundant constituent in all the extracts (4.262 mg/g in the fruit extract, 0.820 mg/g in the leaf extract, and 0.540 mg/g in the root extract). The biological activity (antimicrobial and antibiofilm) of the extracts was tested against 12 microfungi and 12 bacterial strains by the microdilution method. All the extracts exhibited moderate antimicrobial and antibiofilm activity and inhibited the growth of most of the examined microorganisms. The obtained results indicate the potential role of the tested extracts in pharmacy and medicine.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac"",
journal = "Botanica Serbica",
title = "The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts",
number = "1",
volume = "47",
doi = "10.2298/BOTSERB2301093M",
pages = "93-101"
}

Miletić, M., Ivanov, M., Topalović, A., Gavrilović, M., Gašić, U.,& Janaćković, P.. (2023). The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden "Jevremovac"., 47(1), 93-101.
https://doi.org/10.2298/BOTSERB2301093M

Miletić M, Ivanov M, Topalović A, Gavrilović M, Gašić U, Janaćković P. The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts. in Botanica Serbica. 2023;47(1):93-101.
doi:10.2298/BOTSERB2301093M .

Miletić, Milica, Ivanov, Marija, Topalović, Aleksandra, Gavrilović, Milan, Gašić, Uroš, Janaćković, Peđa, "The phenolic constituents and antimicrobial activity of Xanthium spinosum (asteraceae) extracts" in Botanica Serbica, 47, no. 1 (2023):93-101,
https://doi.org/10.2298/BOTSERB2301093M . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6467
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
SP  - 477
ER  - 

@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477",
pages = "477"
}

Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae
Basel: MDPI., 9(4), 477.
https://doi.org/10.3390/horticulturae9040477

Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4):477.
doi:10.3390/horticulturae9040477 .

Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023):477,
https://doi.org/10.3390/horticulturae9040477 . .

TY  - JOUR
AU  - Alimpić-Aradski, Ana
AU  - Olađe Pavlović, Mariana
AU  - Janošević, Dušica
AU  - Todorović, Slađana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Marin, Petar D.
AU  - Giweli, Abdulhmid
AU  - Duletić-Laušević, Sonja
PY  - 2023
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/5152
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6445
AB  - Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
PB  - Hoboken: John Wiley and Sons
T2  - Phytochemical Analysis
T1  - Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
IS  - 6
VL  - 34
DO  - 10.1002/pca.3257
SP  - 661
EP  - 679
ER  - 

@article{
author = "Alimpić-Aradski, Ana and Olađe Pavlović, Mariana and Janošević, Dušica and Todorović, Slađana and Gašić, Uroš and Mišić, Danijela and Pljevljakušić, Dejan and Šavikin, Katarina and Marin, Petar D. and Giweli, Abdulhmid and Duletić-Laušević, Sonja",
year = "2023",
abstract = "Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Phytochemical Analysis",
title = "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)",
number = "6",
volume = "34",
doi = "10.1002/pca.3257",
pages = "661-679"
}

Alimpić-Aradski, A., Olađe Pavlović, M., Janošević, D., Todorović, S., Gašić, U., Mišić, D., Pljevljakušić, D., Šavikin, K., Marin, P. D., Giweli, A.,& Duletić-Laušević, S.. (2023). Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis
Hoboken: John Wiley and Sons., 34(6), 661-679.
https://doi.org/10.1002/pca.3257

Alimpić-Aradski A, Olađe Pavlović M, Janošević D, Todorović S, Gašić U, Mišić D, Pljevljakušić D, Šavikin K, Marin PD, Giweli A, Duletić-Laušević S. Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis. 2023;34(6):661-679.
doi:10.1002/pca.3257 .

Alimpić-Aradski, Ana, Olađe Pavlović, Mariana, Janošević, Dušica, Todorović, Slađana, Gašić, Uroš, Mišić, Danijela, Pljevljakušić, Dejan, Šavikin, Katarina, Marin, Petar D., Giweli, Abdulhmid, Duletić-Laušević, Sonja, "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)" in Phytochemical Analysis, 34, no. 6 (2023):661-679,
https://doi.org/10.1002/pca.3257 . .

TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Ćirić, Ana
AU  - Gašić, Uroš
AU  - Žarković, Lazar D.
AU  - Krivošej, Zoran Đ.
AU  - Džamić, Ana M.
PY  - 2023
UR  - https://www.acgpubs.org/doc/20221125121922364-RNP-2207-2528.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5345
AB  - The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.
T2  - Records of Natural Products
T1  - Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia
IS  - 3
VL  - 17
DO  - 10.25135/rnp.364.2207.2528
SP  - 453
EP  - 475
ER  - 

@article{
author = "Mileski, Ksenija S. and Ćirić, Ana and Gašić, Uroš and Žarković, Lazar D. and Krivošej, Zoran Đ. and Džamić, Ana M.",
year = "2023",
abstract = "The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.",
journal = "Records of Natural Products",
title = "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia",
number = "3",
volume = "17",
doi = "10.25135/rnp.364.2207.2528",
pages = "453-475"
}

Mileski, K. S., Ćirić, A., Gašić, U., Žarković, L. D., Krivošej, Z. Đ.,& Džamić, A. M.. (2023). Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products, 17(3), 453-475.
https://doi.org/10.25135/rnp.364.2207.2528

Mileski KS, Ćirić A, Gašić U, Žarković LD, Krivošej ZĐ, Džamić AM. Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products. 2023;17(3):453-475.
doi:10.25135/rnp.364.2207.2528 .

Mileski, Ksenija S., Ćirić, Ana, Gašić, Uroš, Žarković, Lazar D., Krivošej, Zoran Đ., Džamić, Ana M., "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia" in Records of Natural Products, 17, no. 3 (2023):453-475,
https://doi.org/10.25135/rnp.364.2207.2528 . .

TY  - JOUR
AU  - Korpayev, Serdar
AU  - Hamrayev, Hemra
AU  - Aničić, Neda
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Agamyradov, Mirap
AU  - Agamyradova, Guljan
AU  - Rozyyev, Hangeldi
AU  - Amanov, Gadam
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6371
AB  - The green synthesis of nanoparticles using various plant materials expands the horizons of phytochemists while discouraging the use of toxic chemicals. In this paper, we present a low-cost and environmentally friendly method for producing silver nanoparticles (AgNPs) by using Alhagi persarum flowers (APF) extracts as both a reducing and capping agent for the first time. The bioactive compounds in the extract were characterized using UHPLC-DAD-QqQ-MS/MS, leading to the identification of 18 compounds, including flavonoids, phenolic acids, and alkaloids, with a total phenolic content of 12.17±0.16 mg GAE/g and a total flavonoid content of 23.09±0.33 mg RE/g. The surface plasmon resonance band in UV-vis spectroscopy was used to determine the formation of silver nanoparticles (AgNPs) in the reaction mixture. Fourier transmission infrared (FT-IR) spectroscopy was used to identify the active biomolecules responsible for the reduction and the stabilization of AgNPs synthesized in APF extract. The crystalline structure of metallic silver ions and the presence of pure elemental silver ions were revealed by X-ray diffraction (XRD) and EDX analysis, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to determine the morphology and topography of the AgNPs. Additionally, the synthesized AgNPs were found to have antimicrobial activity against multidrug-resistant Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli and Pseudomonas aeruginosa), and fungus (Candida albicans) clinically isolated human pathogens. Furthermore, the AgNPs were tested for antioxidant activity (DPPH and SO) and cytotoxicity on human breast adenosarcoma cells (MCF-7). Taken together, these results suggest that biosynthesized AgNPs could be used as a potential therapeutic formulation for bacterial infections and breast cancer.
PB  - Heidelberg: Springer Nature
T2  - Biomass Conversion and Biorefinery
T1  - Green synthesis of silver nanoparticles with Alhagi persarum flowers extract and its antioxidant, antimicrobial, and cytotoxic activities
DO  - 10.1007/s13399-023-04648-1
SP  - 1-15
ER  - 

@article{
author = "Korpayev, Serdar and Hamrayev, Hemra and Aničić, Neda and Gašić, Uroš and Zengin, Gokhan and Agamyradov, Mirap and Agamyradova, Guljan and Rozyyev, Hangeldi and Amanov, Gadam",
year = "2023",
abstract = "The green synthesis of nanoparticles using various plant materials expands the horizons of phytochemists while discouraging the use of toxic chemicals. In this paper, we present a low-cost and environmentally friendly method for producing silver nanoparticles (AgNPs) by using Alhagi persarum flowers (APF) extracts as both a reducing and capping agent for the first time. The bioactive compounds in the extract were characterized using UHPLC-DAD-QqQ-MS/MS, leading to the identification of 18 compounds, including flavonoids, phenolic acids, and alkaloids, with a total phenolic content of 12.17±0.16 mg GAE/g and a total flavonoid content of 23.09±0.33 mg RE/g. The surface plasmon resonance band in UV-vis spectroscopy was used to determine the formation of silver nanoparticles (AgNPs) in the reaction mixture. Fourier transmission infrared (FT-IR) spectroscopy was used to identify the active biomolecules responsible for the reduction and the stabilization of AgNPs synthesized in APF extract. The crystalline structure of metallic silver ions and the presence of pure elemental silver ions were revealed by X-ray diffraction (XRD) and EDX analysis, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to determine the morphology and topography of the AgNPs. Additionally, the synthesized AgNPs were found to have antimicrobial activity against multidrug-resistant Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli and Pseudomonas aeruginosa), and fungus (Candida albicans) clinically isolated human pathogens. Furthermore, the AgNPs were tested for antioxidant activity (DPPH and SO) and cytotoxicity on human breast adenosarcoma cells (MCF-7). Taken together, these results suggest that biosynthesized AgNPs could be used as a potential therapeutic formulation for bacterial infections and breast cancer.",
publisher = "Heidelberg: Springer Nature",
journal = "Biomass Conversion and Biorefinery",
title = "Green synthesis of silver nanoparticles with Alhagi persarum flowers extract and its antioxidant, antimicrobial, and cytotoxic activities",
doi = "10.1007/s13399-023-04648-1",
pages = "1-15"
}

Korpayev, S., Hamrayev, H., Aničić, N., Gašić, U., Zengin, G., Agamyradov, M., Agamyradova, G., Rozyyev, H.,& Amanov, G.. (2023). Green synthesis of silver nanoparticles with Alhagi persarum flowers extract and its antioxidant, antimicrobial, and cytotoxic activities. in Biomass Conversion and Biorefinery
Heidelberg: Springer Nature., 1-15.
https://doi.org/10.1007/s13399-023-04648-1

Korpayev S, Hamrayev H, Aničić N, Gašić U, Zengin G, Agamyradov M, Agamyradova G, Rozyyev H, Amanov G. Green synthesis of silver nanoparticles with Alhagi persarum flowers extract and its antioxidant, antimicrobial, and cytotoxic activities. in Biomass Conversion and Biorefinery. 2023;:1-15.
doi:10.1007/s13399-023-04648-1 .

Korpayev, Serdar, Hamrayev, Hemra, Aničić, Neda, Gašić, Uroš, Zengin, Gokhan, Agamyradov, Mirap, Agamyradova, Guljan, Rozyyev, Hangeldi, Amanov, Gadam, "Green synthesis of silver nanoparticles with Alhagi persarum flowers extract and its antioxidant, antimicrobial, and cytotoxic activities" in Biomass Conversion and Biorefinery (2023):1-15,
https://doi.org/10.1007/s13399-023-04648-1 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Mačukanović-Jocić, Marina
AU  - Radošević, Radenko
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6306
AB  - The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040424
SP  - 424
ER  - 

@article{
author = "Fotirić Akšić, Milica and Mačukanović-Jocić, Marina and Radošević, Radenko and Nedić, Nebojša and Gašić, Uroš and Tosti, Tomislav and Tešić, Živoslav and Meland, Mekjell",
year = "2023",
abstract = "The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040424",
pages = "424"
}

Fotirić Akšić, M., Mačukanović-Jocić, M., Radošević, R., Nedić, N., Gašić, U., Tosti, T., Tešić, Ž.,& Meland, M.. (2023). The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae
Basel: MDPI., 9(4), 424.
https://doi.org/10.3390/horticulturae9040424

Fotirić Akšić M, Mačukanović-Jocić M, Radošević R, Nedić N, Gašić U, Tosti T, Tešić Ž, Meland M. The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae. 2023;9(4):424.
doi:10.3390/horticulturae9040424 .

Fotirić Akšić, Milica, Mačukanović-Jocić, Marina, Radošević, Radenko, Nedić, Nebojša, Gašić, Uroš, Tosti, Tomislav, Tešić, Živoslav, Meland, Mekjell, "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora" in Horticulturae, 9, no. 4 (2023):424,
https://doi.org/10.3390/horticulturae9040424 . .

TY  - JOUR
AU  - Gašić, Uroš
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1155297/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5494
AB  - The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .
T2  - Frontiers in Plant Science
T1  - Variation in the chemical profiles of three foxglove species in the central Balkans
VL  - 14
DO  - 10.3389/fpls.2023.1155297
ER  - 

@article{
author = "Gašić, Uroš and Banjanac, Tijana and Šiler, Branislav and Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Dmitrović, Slavica and Skorić, Marijana and Nestorović Živković, Jasmina and Petrović, Luka and Todorović, Miloš and Živković, Suzana and Matekalo, Dragana and Filipović, Biljana and Lukić, Tamara and Mišić, Danijela",
year = "2023",
abstract = "The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .",
journal = "Frontiers in Plant Science",
title = "Variation in the chemical profiles of three foxglove species in the central Balkans",
volume = "14",
doi = "10.3389/fpls.2023.1155297"
}

Gašić, U., Banjanac, T., Šiler, B., Božunović, J., Milutinović, M., Aničić, N., Dmitrović, S., Skorić, M., Nestorović Živković, J., Petrović, L., Todorović, M., Živković, S., Matekalo, D., Filipović, B., Lukić, T.,& Mišić, D.. (2023). Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science, 14.
https://doi.org/10.3389/fpls.2023.1155297

Gašić U, Banjanac T, Šiler B, Božunović J, Milutinović M, Aničić N, Dmitrović S, Skorić M, Nestorović Živković J, Petrović L, Todorović M, Živković S, Matekalo D, Filipović B, Lukić T, Mišić D. Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science. 2023;14.
doi:10.3389/fpls.2023.1155297 .

Gašić, Uroš, Banjanac, Tijana, Šiler, Branislav, Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Dmitrović, Slavica, Skorić, Marijana, Nestorović Živković, Jasmina, Petrović, Luka, Todorović, Miloš, Živković, Suzana, Matekalo, Dragana, Filipović, Biljana, Lukić, Tamara, Mišić, Danijela, "Variation in the chemical profiles of three foxglove species in the central Balkans" in Frontiers in Plant Science, 14 (2023),
https://doi.org/10.3389/fpls.2023.1155297 . .