TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Mijušković, Ana
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Miljević, Čedo
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4042
AB  - The use of atypical antipsychotic drugs (APD) was reported to be associated with adverse effectson the kidneys. Thus, the aim of this study was to examine whether APD exerted their adverseeffects by interfering with the renal antioxidant defense system. Male 3-mo-old Wistar rats weretreated for 28 d with ziprasidone (ZIP), clozapine (CLO), or sertindole (SER) using a daily doserecommended for antipsychotic drug therapy. The expression and activities of antioxidantenzymes superoxide dismutase (SOD) type 1 and type 2, catalase (CAT), glutathione reductase(GR), and glutathione S-transferases (GSTs) activity were measured in the kidneys. Changes in thekidneys were also evaluated histologically. Ziprasidone, CLO, and SER reduced renal SOD type 1and type 2 activities. Decreased CAT activity was observed only in SER-treated rats. An inhibitionin GR activity and increased activity of GST was found only after treatment with CLO. Histologicalanalysis showed dilatation of proximal tubules in kidneys with all three drugs. In conclusion, dataindicate that redox disturbances may contribute to renal morphologic alterations in proximaltubules in rats treated with all APD.
PB  - Taylor & Francis
T2  - Journal of Toxicology and Environmental Health, Part A: Current Issues
T1  - Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney
IS  - 20
VL  - 79
DO  - 10.1080/15287394.2016.1201706
SP  - 905
EP  - 911
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Mijušković, Ana and Tatalović, Nikola and Brkljačić, Jelena and Miler, Marko and Oreščanin Dušić, Zorana and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Mihajlo and Miljević, Čedo",
year = "2016",
abstract = "The use of atypical antipsychotic drugs (APD) was reported to be associated with adverse effectson the kidneys. Thus, the aim of this study was to examine whether APD exerted their adverseeffects by interfering with the renal antioxidant defense system. Male 3-mo-old Wistar rats weretreated for 28 d with ziprasidone (ZIP), clozapine (CLO), or sertindole (SER) using a daily doserecommended for antipsychotic drug therapy. The expression and activities of antioxidantenzymes superoxide dismutase (SOD) type 1 and type 2, catalase (CAT), glutathione reductase(GR), and glutathione S-transferases (GSTs) activity were measured in the kidneys. Changes in thekidneys were also evaluated histologically. Ziprasidone, CLO, and SER reduced renal SOD type 1and type 2 activities. Decreased CAT activity was observed only in SER-treated rats. An inhibitionin GR activity and increased activity of GST was found only after treatment with CLO. Histologicalanalysis showed dilatation of proximal tubules in kidneys with all three drugs. In conclusion, dataindicate that redox disturbances may contribute to renal morphologic alterations in proximaltubules in rats treated with all APD.",
publisher = "Taylor & Francis",
journal = "Journal of Toxicology and Environmental Health, Part A: Current Issues",
title = "Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney",
number = "20",
volume = "79",
doi = "10.1080/15287394.2016.1201706",
pages = "905-911"
}

Nikolić-Kokić, A., Mijušković, A., Tatalović, N., Brkljačić, J., Miler, M., Oreščanin Dušić, Z., Nikolić, M., Milošević, V., Blagojević, D., Spasić, M.,& Miljević, Č.. (2016). Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney. in Journal of Toxicology and Environmental Health, Part A: Current Issues
Taylor & Francis., 79(20), 905-911.
https://doi.org/10.1080/15287394.2016.1201706

Nikolić-Kokić A, Mijušković A, Tatalović N, Brkljačić J, Miler M, Oreščanin Dušić Z, Nikolić M, Milošević V, Blagojević D, Spasić M, Miljević Č. Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney. in Journal of Toxicology and Environmental Health, Part A: Current Issues. 2016;79(20):905-911.
doi:10.1080/15287394.2016.1201706 .

Nikolić-Kokić, Aleksandra, Mijušković, Ana, Tatalović, Nikola, Brkljačić, Jelena, Miler, Marko, Oreščanin Dušić, Zorana, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Mihajlo, Miljević, Čedo, "Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney" in Journal of Toxicology and Environmental Health, Part A: Current Issues, 79, no. 20 (2016):905-911,
https://doi.org/10.1080/15287394.2016.1201706 . .

TY  - CONF
AU  - Mijušković, Ana
AU  - Tatalović, Nikola
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4043
AB  - The uterus is a spontaneously active tissue, whose contractions have to be controlled and
regulated for successful pregnancy and parturition. Spontaneous contractions might be initiated
by spontaneous pacemaker activity, although pacemaker cells are not fully defined. Myometrial
membrane potential changes are essential for uterine activity which is a function of ion movement
across the membrane governed by ion channel activity [1]. Changes in membrane potential are
achieved by coordinated actions of two classes of channels: those that exert hyperpolarizing
current and those that exert depolarizing currents. Calcium-activated chloride channels (CaCCs)
are important contributors to depolarizing currents 1,2. However, the molecular identity of CaCCs
is still not known. Several candidates have been proposed including bestrophins (BEST), CLCA
and the anoctamin (ANO or TMEM) family of proteins 3.
Improper or irregular uterine activity may underlie the common pathological disorders such
as infertility, improper implantation, dysmenorrhea, weak uterine contraction during labor and
preterm labor. The contractile activity of the uterus is regulated by the complex electrophysiologic
network which is highly sensitive to various pharmacological and signaling molecules.
Hydrogen sulphide (H2S) appears to be an important signaling molecule in rat uterus. The
production of H2S and the presence of enzymes responsible for its endogenous production
(cystathionine beta-synthase and cystathionine gamma-lyase) have been demonstrated in rat
uterus 3,4. Hydrogen sulphide reduces uterine contractility, and it is recognized as a promising
treatment for uterine disorders. It decreases amplitude as well as frequency of uterine
contractions. H2S effect on the frequency of contractions appears to be mediated via pacemaker
channels. CaCCs channel inhibitors were shown to reduce the frequency of spontaneous
contractions in myometrial strips and were proposed to be the main pacemaker channels in
smooth muscles 1. Very little, however, is known about these channels in the myometrium.
The mechanism of the H2S -induced relaxation in non-pregnant uteri has not been examined.
Organ bath studies were employed to assess the pharmacological effects of sodium sulphide
(Na2S; hydrogen sulphide donor) in uterine strips by exposing them to Na2S with or without
Cl− channel blockers (DIDS, NFA, T16Ainh-A01, TA). Relaxation was not affected by
majority of CaCC modulators since T16Ainh-AO1, tannic acid and NFA failed to inhibit
Na2S induced relaxation but is DIDS sensitive 3. DIDS was recently found to be highly
selective for bestrophin (BEST-1) channels 5.
BEST proteins were shown to recapitulate the properties of native CaCCs. Although
both bestrophin and calcium-activated chloride channel families were proposed to be the
candidate genes for smooth muscle contraction their exact function and regulation remain
to be confirmed 6.
The aim of this study was to explore the expression of bestrophins channels (BEST-1
and BEST-2) in rat uterus in estrus. Expression studies of the BEST-1 and BEST-2 were
performed by Western blotting, RT-PCR and immunohistochemistry. BEST-1 and BEST-2
are expresses at the mRNA level and at protein level in rat uterus in estrus, suggesting a role
for BESTs in the control of uterine contractility. However, expression of BEST-1 is higher
comparing to BEST-2. Moreover, BEST-1 seems to be major mediators of Na2S induced
uterine relaxation. Mechanistic insights of possible Na2S-induced modulation od BEST-1
were performed by molecular docking studies.
Taken together, work undertaken strengthens the evidence of a physiologically important
role for bestrophin channels in the normal physiology of uterine contractions. Moreover, is
an important modulator of uterine contractions and bestrophins appear to be main modulator
of its effects. This research add to our understanding of molecular mechanisms of H2S effects
and will be beneficial in designing future in vivo studies, and ultimately identifying new
therapeutic targets to treat uterine disorders that are associated with disturbed contractility.
PB  - Belgrade : Faculty of Chemistry
PB  - Belgrade : Serbian Biochemical Society
C3  - Serbian Biochemical Society  Fifth Conference; Integrated research in life science; Proceedings
T1  - Calcium-activated chloride channels and Na2S-induced relaxation of non-pregnant rat uteri in estrus
SP  - 111
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4043
ER  - 

@conference{
author = "Mijušković, Ana and Tatalović, Nikola and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Spasić, Mihajlo and Blagojević, Duško",
year = "2015",
abstract = "The uterus is a spontaneously active tissue, whose contractions have to be controlled and
regulated for successful pregnancy and parturition. Spontaneous contractions might be initiated
by spontaneous pacemaker activity, although pacemaker cells are not fully defined. Myometrial
membrane potential changes are essential for uterine activity which is a function of ion movement
across the membrane governed by ion channel activity [1]. Changes in membrane potential are
achieved by coordinated actions of two classes of channels: those that exert hyperpolarizing
current and those that exert depolarizing currents. Calcium-activated chloride channels (CaCCs)
are important contributors to depolarizing currents 1,2. However, the molecular identity of CaCCs
is still not known. Several candidates have been proposed including bestrophins (BEST), CLCA
and the anoctamin (ANO or TMEM) family of proteins 3.
Improper or irregular uterine activity may underlie the common pathological disorders such
as infertility, improper implantation, dysmenorrhea, weak uterine contraction during labor and
preterm labor. The contractile activity of the uterus is regulated by the complex electrophysiologic
network which is highly sensitive to various pharmacological and signaling molecules.
Hydrogen sulphide (H2S) appears to be an important signaling molecule in rat uterus. The
production of H2S and the presence of enzymes responsible for its endogenous production
(cystathionine beta-synthase and cystathionine gamma-lyase) have been demonstrated in rat
uterus 3,4. Hydrogen sulphide reduces uterine contractility, and it is recognized as a promising
treatment for uterine disorders. It decreases amplitude as well as frequency of uterine
contractions. H2S effect on the frequency of contractions appears to be mediated via pacemaker
channels. CaCCs channel inhibitors were shown to reduce the frequency of spontaneous
contractions in myometrial strips and were proposed to be the main pacemaker channels in
smooth muscles 1. Very little, however, is known about these channels in the myometrium.
The mechanism of the H2S -induced relaxation in non-pregnant uteri has not been examined.
Organ bath studies were employed to assess the pharmacological effects of sodium sulphide
(Na2S; hydrogen sulphide donor) in uterine strips by exposing them to Na2S with or without
Cl− channel blockers (DIDS, NFA, T16Ainh-A01, TA). Relaxation was not affected by
majority of CaCC modulators since T16Ainh-AO1, tannic acid and NFA failed to inhibit
Na2S induced relaxation but is DIDS sensitive 3. DIDS was recently found to be highly
selective for bestrophin (BEST-1) channels 5.
BEST proteins were shown to recapitulate the properties of native CaCCs. Although
both bestrophin and calcium-activated chloride channel families were proposed to be the
candidate genes for smooth muscle contraction their exact function and regulation remain
to be confirmed 6.
The aim of this study was to explore the expression of bestrophins channels (BEST-1
and BEST-2) in rat uterus in estrus. Expression studies of the BEST-1 and BEST-2 were
performed by Western blotting, RT-PCR and immunohistochemistry. BEST-1 and BEST-2
are expresses at the mRNA level and at protein level in rat uterus in estrus, suggesting a role
for BESTs in the control of uterine contractility. However, expression of BEST-1 is higher
comparing to BEST-2. Moreover, BEST-1 seems to be major mediators of Na2S induced
uterine relaxation. Mechanistic insights of possible Na2S-induced modulation od BEST-1
were performed by molecular docking studies.
Taken together, work undertaken strengthens the evidence of a physiologically important
role for bestrophin channels in the normal physiology of uterine contractions. Moreover, is
an important modulator of uterine contractions and bestrophins appear to be main modulator
of its effects. This research add to our understanding of molecular mechanisms of H2S effects
and will be beneficial in designing future in vivo studies, and ultimately identifying new
therapeutic targets to treat uterine disorders that are associated with disturbed contractility.",
publisher = "Belgrade : Faculty of Chemistry, Belgrade : Serbian Biochemical Society",
journal = "Serbian Biochemical Society  Fifth Conference; Integrated research in life science; Proceedings",
title = "Calcium-activated chloride channels and Na2S-induced relaxation of non-pregnant rat uteri in estrus",
pages = "111-112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4043"
}

Mijušković, A., Tatalović, N., Nikolić-Kokić, A., Oreščanin Dušić, Z., Spasić, M.,& Blagojević, D.. (2015). Calcium-activated chloride channels and Na2S-induced relaxation of non-pregnant rat uteri in estrus. in Serbian Biochemical Society  Fifth Conference; Integrated research in life science; Proceedings
Belgrade : Faculty of Chemistry., 111-112.
https://hdl.handle.net/21.15107/rcub_ibiss_4043

Mijušković A, Tatalović N, Nikolić-Kokić A, Oreščanin Dušić Z, Spasić M, Blagojević D. Calcium-activated chloride channels and Na2S-induced relaxation of non-pregnant rat uteri in estrus. in Serbian Biochemical Society  Fifth Conference; Integrated research in life science; Proceedings. 2015;:111-112.
https://hdl.handle.net/21.15107/rcub_ibiss_4043 .

Mijušković, Ana, Tatalović, Nikola, Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Spasić, Mihajlo, Blagojević, Duško, "Calcium-activated chloride channels and Na2S-induced relaxation of non-pregnant rat uteri in estrus" in Serbian Biochemical Society  Fifth Conference; Integrated research in life science; Proceedings (2015):111-112,
https://hdl.handle.net/21.15107/rcub_ibiss_4043 .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Oreščanin Dušić, Zorana
AU  - Slavić, Marija
AU  - Spasojević, Ivan
AU  - Stević, Zorica
AU  - Spasić, Mihajlo
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/504
AB  - Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.
AB  - Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p<0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.
T2  - Journal of Medical Biochemistry
T1  - Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića
T1  - The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions
IS  - 4
VL  - 32
SP  - 375
EP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_504
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Blagojević, Duško and Oreščanin Dušić, Zorana and Slavić, Marija and Spasojević, Ivan and Stević, Zorica and Spasić, Mihajlo",
year = "2013, 2013",
abstract = "Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS., Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p<0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.",
journal = "Journal of Medical Biochemistry",
title = "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića, The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions",
number = "4",
volume = "32",
pages = "375-379",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_504"
}

Nikolić-Kokić, A., Blagojević, D., Oreščanin Dušić, Z., Slavić, M., Spasojević, I., Stević, Z.,& Spasić, M.. (2013). Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry, 32(4), 375-379.
https://hdl.handle.net/21.15107/rcub_ibiss_504

Nikolić-Kokić A, Blagojević D, Oreščanin Dušić Z, Slavić M, Spasojević I, Stević Z, Spasić M. Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry. 2013;32(4):375-379.
https://hdl.handle.net/21.15107/rcub_ibiss_504 .

Nikolić-Kokić, Aleksandra, Blagojević, Duško, Oreščanin Dušić, Zorana, Slavić, Marija, Spasojević, Ivan, Stević, Zorica, Spasić, Mihajlo, "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića" in Journal of Medical Biochemistry, 32, no. 4 (2013):375-379,
https://hdl.handle.net/21.15107/rcub_ibiss_504 .

TY  - CONF
AU  - Velkovski, Saško
AU  - Ristić, Snežana
AU  - Milošević, Verica
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
AU  - Pavlović, Slađan
AU  - Nikolić, Aleksandra
AU  - Blagojević, Duško
AU  - Starčević, Vesna
PY  - 2002
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5958
AB  - Postoji veli ki broj podataka o pokusajima uspostavljanja pouzdanog animalnog modela atcroskleroze, ali jos n ije poznat  dovoljno  dobar  analog  humanoj  aterosk lerozi. Kao prilog ovim is trazivanjima, pokusali smo da proce­ nimo doprinos  reaktivnih  vrsta  kiseonika  (ROS)  u  ranoj ate rogenezi i  prevcntivnu  ulogu  cndogenog  antioksida ­ cio nog zastitnog sis tema (AOS). Odrasli muzjaci Wistar albin o pacova, te les ne masc  oko  400  g,  korisceni  su  za sp rovodenje specijalne ate rogenc dijctc  koja se sas toja la od  smcse  masti  ( buter,  holesterol)  i  drugih   sup stanc i ( ho ln a kise li na, holi n hlorid, tiourac i l ). Kontrolnc i eks­ per imenta lne zivo tin je su bi le srnestcne u plasticne  ka­ veze  i  imale  s u  dostu pne  peleti ranu   hranu  i  vodu po volji. Eksperimentalne zivotinje su dobijale svakoga dana u toku 8 nedeija po 5 g aterogene smese, sondom, intragastricno. Na  kraju  dijete  sve zivotinje  su zrtvovane i sakupljena je krv za odredivanje (1) lipidnog statusa: koncentracija triglicerida (TG), ukupnog holesterola (CH). LDL holesterola i HDL holesterola u plazmi, od­ nosa  koncentracija  LDL i HDL  holesterola  (LDL/HDL) i (2) antioksidacionog statusa u krvi: aktivnost kata­ laze u eritrocitima (Er CAT), aktivnost glutation-S-tran­ sferaze (GST) u  plazmi,  koncentracija  glutationa  (GSH) u plazmi i koncentracija vitamina E (Vit. E) u plazmi. Uzimani su isecci abdominalne aorte za histolosku veri­ fikaciju ateroskle roze. Razlike izmedu vrednosti kon­ trolnih i eksperimentalnih zivotinja testirane su  stati­ sticki.
Analiza hitoloskih praparata aorte pokazala je normalnu gradu arterijskog zida u kontrolnih zivotinja , dok su u eksperimentalnih zivotinja nadene inicijalne promene u intimi (zadebljanje i  celijska akumulacija). Vrednosti svih pracenih varijabli lipidnog i  antioksidacionog  sta­ tusa u kontrolnih zivotinja bile su normalne. U eksperi­ inentalnih zivotinja postojalo je statisticki znacajno po­ vecanje koncentracije TG  (p < 0,05),  koncentracije  CH (p < 0,01), koncentracije LDL (p < 0,01) i odnosa LDL/HDL (p < 0,01) u poredenju sa kontrolnim zivoti­ njama . Koncentracija HDL bila je nepromenjena (p > 0,05). Aktivnost Er CAT i koncentracija  GSH  u plazmi bile su povecane  u  eksperimentalnih  zivotinja  u odnosu na kontrolne,  ali  ne znacajno (p > 0,05). Aktivnost  GST  u plazmi nije bila promenjena (p > 0,05). Samo kon­ centracija Vit. E u  plazmi eksperimentalnih  zivotinja bila je statisticki znacajno veca  u poredenju  sa  kontrol­ nim zivotinjama (p < 0,01). Mozemo zakljuciti da anti­ oksidacioni status u krvi nije bio u korelaciji sa lipidnim statusom  u toku rane aterogeneze  u odraslih  pacova.
PB  - Priština: Academy of Sciences and Arts of SAP Kosovo. Division of Natural Sciences: Society for Biology and Experimental Medicine SAP Kosovo
C3  - Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia
T1  - Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5958
ER  - 

@conference{
author = "Velkovski, Saško and Ristić, Snežana and Milošević, Verica and Saičić, Zorica and Spasić, Mihajlo and Pavlović, Slađan and Nikolić, Aleksandra and Blagojević, Duško and Starčević, Vesna",
year = "2002",
abstract = "Postoji veli ki broj podataka o pokusajima uspostavljanja pouzdanog animalnog modela atcroskleroze, ali jos n ije poznat  dovoljno  dobar  analog  humanoj  aterosk lerozi. Kao prilog ovim is trazivanjima, pokusali smo da proce­ nimo doprinos  reaktivnih  vrsta  kiseonika  (ROS)  u  ranoj ate rogenezi i  prevcntivnu  ulogu  cndogenog  antioksida ­ cio nog zastitnog sis tema (AOS). Odrasli muzjaci Wistar albin o pacova, te les ne masc  oko  400  g,  korisceni  su  za sp rovodenje specijalne ate rogenc dijctc  koja se sas toja la od  smcse  masti  ( buter,  holesterol)  i  drugih   sup stanc i ( ho ln a kise li na, holi n hlorid, tiourac i l ). Kontrolnc i eks­ per imenta lne zivo tin je su bi le srnestcne u plasticne  ka­ veze  i  imale  s u  dostu pne  peleti ranu   hranu  i  vodu po volji. Eksperimentalne zivotinje su dobijale svakoga dana u toku 8 nedeija po 5 g aterogene smese, sondom, intragastricno. Na  kraju  dijete  sve zivotinje  su zrtvovane i sakupljena je krv za odredivanje (1) lipidnog statusa: koncentracija triglicerida (TG), ukupnog holesterola (CH). LDL holesterola i HDL holesterola u plazmi, od­ nosa  koncentracija  LDL i HDL  holesterola  (LDL/HDL) i (2) antioksidacionog statusa u krvi: aktivnost kata­ laze u eritrocitima (Er CAT), aktivnost glutation-S-tran­ sferaze (GST) u  plazmi,  koncentracija  glutationa  (GSH) u plazmi i koncentracija vitamina E (Vit. E) u plazmi. Uzimani su isecci abdominalne aorte za histolosku veri­ fikaciju ateroskle roze. Razlike izmedu vrednosti kon­ trolnih i eksperimentalnih zivotinja testirane su  stati­ sticki.
Analiza hitoloskih praparata aorte pokazala je normalnu gradu arterijskog zida u kontrolnih zivotinja , dok su u eksperimentalnih zivotinja nadene inicijalne promene u intimi (zadebljanje i  celijska akumulacija). Vrednosti svih pracenih varijabli lipidnog i  antioksidacionog  sta­ tusa u kontrolnih zivotinja bile su normalne. U eksperi­ inentalnih zivotinja postojalo je statisticki znacajno po­ vecanje koncentracije TG  (p < 0,05),  koncentracije  CH (p < 0,01), koncentracije LDL (p < 0,01) i odnosa LDL/HDL (p < 0,01) u poredenju sa kontrolnim zivoti­ njama . Koncentracija HDL bila je nepromenjena (p > 0,05). Aktivnost Er CAT i koncentracija  GSH  u plazmi bile su povecane  u  eksperimentalnih  zivotinja  u odnosu na kontrolne,  ali  ne znacajno (p > 0,05). Aktivnost  GST  u plazmi nije bila promenjena (p > 0,05). Samo kon­ centracija Vit. E u  plazmi eksperimentalnih  zivotinja bila je statisticki znacajno veca  u poredenju  sa  kontrol­ nim zivotinjama (p < 0,01). Mozemo zakljuciti da anti­ oksidacioni status u krvi nije bio u korelaciji sa lipidnim statusom  u toku rane aterogeneze  u odraslih  pacova.",
publisher = "Priština: Academy of Sciences and Arts of SAP Kosovo. Division of Natural Sciences: Society for Biology and Experimental Medicine SAP Kosovo",
journal = "Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia",
title = "Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5958"
}

Velkovski, S., Ristić, S., Milošević, V., Saičić, Z., Spasić, M., Pavlović, S., Nikolić, A., Blagojević, D.,& Starčević, V.. (2002). Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze. in Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia
Priština: Academy of Sciences and Arts of SAP Kosovo. Division of Natural Sciences: Society for Biology and Experimental Medicine SAP Kosovo., 64.
https://hdl.handle.net/21.15107/rcub_ibiss_5958

Velkovski S, Ristić S, Milošević V, Saičić Z, Spasić M, Pavlović S, Nikolić A, Blagojević D, Starčević V. Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze. in Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia. 2002;:64.
https://hdl.handle.net/21.15107/rcub_ibiss_5958 .

Velkovski, Saško, Ristić, Snežana, Milošević, Verica, Saičić, Zorica, Spasić, Mihajlo, Pavlović, Slađan, Nikolić, Aleksandra, Blagojević, Duško, Starčević, Vesna, "Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze" in Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia (2002):64,
https://hdl.handle.net/21.15107/rcub_ibiss_5958 .