TY  - JOUR
AU  - Bogdanović, Milica
AU  - Cankar, Katarina
AU  - Dragićević, Milan
AU  - Bouwmeester, Harro
AU  - Beekwilder, Jules
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2020
UR  - https://www.tandfonline.com/doi/full/10.1080/21645698.2019.1681868
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3520
AB  - Chicory (Cichorium intybus L.) is a medicinal and industrial plant from the Asteraceae family that produces a variety of sesquiterpene lactones (STLs), most importantly bitter guaianolides: lactucin, lactucopicrin and 8-deoxylactucin as well as their modified forms such as oxalates. These compounds have medicinal properties; however, they also hamper the extraction of inulin - a very important food industry product from chicory roots. The first step in guaianolide biosynthesis is catalyzed by germacrene A synthase (GAS) which in chicory exists in two isoforms - GAS long (encoded by CiGASlo) and GAS short (encoded by CiGASsh). AmiRNA silencing was used to obtain plants with reduced GAS gene expression and level of downstream metabolites, guaianolide-15-oxalates, as the major STLs in chicory. This approach could be beneficial for engineering new chicory varieties with varying STL content, and especially varieties with reduced bitter compounds more suitable for inulin production.
T2  - GM Crops & Food
T1  - Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.
IS  - 1
VL  - 11
DO  - 10.1080/21645698.2019.1681868
SP  - 54
EP  - 66
ER  - 

@article{
author = "Bogdanović, Milica and Cankar, Katarina and Dragićević, Milan and Bouwmeester, Harro and Beekwilder, Jules and Simonović, Ana and Todorović, Slađana",
year = "2020",
abstract = "Chicory (Cichorium intybus L.) is a medicinal and industrial plant from the Asteraceae family that produces a variety of sesquiterpene lactones (STLs), most importantly bitter guaianolides: lactucin, lactucopicrin and 8-deoxylactucin as well as their modified forms such as oxalates. These compounds have medicinal properties; however, they also hamper the extraction of inulin - a very important food industry product from chicory roots. The first step in guaianolide biosynthesis is catalyzed by germacrene A synthase (GAS) which in chicory exists in two isoforms - GAS long (encoded by CiGASlo) and GAS short (encoded by CiGASsh). AmiRNA silencing was used to obtain plants with reduced GAS gene expression and level of downstream metabolites, guaianolide-15-oxalates, as the major STLs in chicory. This approach could be beneficial for engineering new chicory varieties with varying STL content, and especially varieties with reduced bitter compounds more suitable for inulin production.",
journal = "GM Crops & Food",
title = "Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.",
number = "1",
volume = "11",
doi = "10.1080/21645698.2019.1681868",
pages = "54-66"
}

Bogdanović, M., Cankar, K., Dragićević, M., Bouwmeester, H., Beekwilder, J., Simonović, A.,& Todorović, S.. (2020). Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.. in GM Crops & Food, 11(1), 54-66.
https://doi.org/10.1080/21645698.2019.1681868

Bogdanović M, Cankar K, Dragićević M, Bouwmeester H, Beekwilder J, Simonović A, Todorović S. Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.. in GM Crops & Food. 2020;11(1):54-66.
doi:10.1080/21645698.2019.1681868 .

Bogdanović, Milica, Cankar, Katarina, Dragićević, Milan, Bouwmeester, Harro, Beekwilder, Jules, Simonović, Ana, Todorović, Slađana, "Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L." in GM Crops & Food, 11, no. 1 (2020):54-66,
https://doi.org/10.1080/21645698.2019.1681868 . .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Cankar, Katarina
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - van Houwelingen, Adele
AU  - Schijlen, Elio
AU  - Schipper, Bert
AU  - Gagneul, David
AU  - Hendriks, Theo
AU  - Quillet, Marie-Christine
AU  - Bouwmeester, Harro
AU  - Bosch, Dirk
AU  - Beekwilder, Jules
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S092666901831063X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3212
AB  - Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis of leaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.
T2  - Industrial Crops and Products
T1  - Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)
VL  - 129
DO  - 10.1016/J.INDCROP.2018.12.011
SP  - 253
EP  - 260
ER  - 

@article{
author = "Bogdanović, Milica and Cankar, Katarina and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and van Houwelingen, Adele and Schijlen, Elio and Schipper, Bert and Gagneul, David and Hendriks, Theo and Quillet, Marie-Christine and Bouwmeester, Harro and Bosch, Dirk and Beekwilder, Jules",
year = "2019",
abstract = "Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis of leaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.",
journal = "Industrial Crops and Products",
title = "Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)",
volume = "129",
doi = "10.1016/J.INDCROP.2018.12.011",
pages = "253-260"
}

Bogdanović, M., Cankar, K., Todorović, S., Dragićević, M., Simonović, A., van Houwelingen, A., Schijlen, E., Schipper, B., Gagneul, D., Hendriks, T., Quillet, M., Bouwmeester, H., Bosch, D.,& Beekwilder, J.. (2019). Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae). in Industrial Crops and Products, 129, 253-260.
https://doi.org/10.1016/J.INDCROP.2018.12.011

Bogdanović M, Cankar K, Todorović S, Dragićević M, Simonović A, van Houwelingen A, Schijlen E, Schipper B, Gagneul D, Hendriks T, Quillet M, Bouwmeester H, Bosch D, Beekwilder J. Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae). in Industrial Crops and Products. 2019;129:253-260.
doi:10.1016/J.INDCROP.2018.12.011 .

Bogdanović, Milica, Cankar, Katarina, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, van Houwelingen, Adele, Schijlen, Elio, Schipper, Bert, Gagneul, David, Hendriks, Theo, Quillet, Marie-Christine, Bouwmeester, Harro, Bosch, Dirk, Beekwilder, Jules, "Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)" in Industrial Crops and Products, 129 (2019):253-260,
https://doi.org/10.1016/J.INDCROP.2018.12.011 . .

TY  - CONF
AU  - Cankar, Katarina
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - van Houwelingen, Adele
AU  - Schijlen, Elio
AU  - van de Geest, Henri
AU  - Hendriks, Theo
AU  - Quillet, MC
AU  - Bouwmeester, Harro
AU  - Bosch, Dirk
AU  - Beekwilder, Jules
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5441
AB  - Sesquiterpene lactones (STLs) give chicory its characteristic bitter taste and are highly concentrated in the latex channels of chicory taproot. The study of STLs is interesting to control the bitterness of industrial and consumption chicory, including witloof. The major STLs in chicory are lactucin, deoxylactucin, and lactucopicrin and oxalate and sulphate conjugates thereof. Formation of chicory STLs requires the biosynthesis of costunolide. Two germacrene A synthases, two germacrene A oxidases and a costunolide synthase have been described from chicory. In this work, the genetic organization of the genes involved in STL biosynthesis in chicory linkage groups is described. A number of genomic regions hosting STL biosynthetic genes have been addressed by NGS sequencing. Gene expression in different tissues is addressed by following their expression
using an RNAseq approach and by promoter activity studies using GFP. Finally, the downregulation of STL gene expression in chicory is achieved in hairy root culture regenerants expressing amiRNAs, and the consequences for STL content in different tissues is tested. These results will contribute to successful breeding strategies for chicory germplasm with well-defined STL content and bitterness.
PB  - Ghent : Phytochemical Society of Europe
C3  - Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium
T1  - Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5441
ER  - 

@conference{
author = "Cankar, Katarina and Bogdanović, Milica and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and van Houwelingen, Adele and Schijlen, Elio and van de Geest, Henri and Hendriks, Theo and Quillet, MC and Bouwmeester, Harro and Bosch, Dirk and Beekwilder, Jules",
year = "2016",
abstract = "Sesquiterpene lactones (STLs) give chicory its characteristic bitter taste and are highly concentrated in the latex channels of chicory taproot. The study of STLs is interesting to control the bitterness of industrial and consumption chicory, including witloof. The major STLs in chicory are lactucin, deoxylactucin, and lactucopicrin and oxalate and sulphate conjugates thereof. Formation of chicory STLs requires the biosynthesis of costunolide. Two germacrene A synthases, two germacrene A oxidases and a costunolide synthase have been described from chicory. In this work, the genetic organization of the genes involved in STL biosynthesis in chicory linkage groups is described. A number of genomic regions hosting STL biosynthetic genes have been addressed by NGS sequencing. Gene expression in different tissues is addressed by following their expression
using an RNAseq approach and by promoter activity studies using GFP. Finally, the downregulation of STL gene expression in chicory is achieved in hairy root culture regenerants expressing amiRNAs, and the consequences for STL content in different tissues is tested. These results will contribute to successful breeding strategies for chicory germplasm with well-defined STL content and bitterness.",
publisher = "Ghent : Phytochemical Society of Europe",
journal = "Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium",
title = "Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5441"
}

Cankar, K., Bogdanović, M., Todorović, S., Dragićević, M., Simonović, A., van Houwelingen, A., Schijlen, E., van de Geest, H., Hendriks, T., Quillet, M., Bouwmeester, H., Bosch, D.,& Beekwilder, J.. (2016). Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory. in Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium
Ghent : Phytochemical Society of Europe., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_5441

Cankar K, Bogdanović M, Todorović S, Dragićević M, Simonović A, van Houwelingen A, Schijlen E, van de Geest H, Hendriks T, Quillet M, Bouwmeester H, Bosch D, Beekwilder J. Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory. in Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium. 2016;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_5441 .

Cankar, Katarina, Bogdanović, Milica, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, van Houwelingen, Adele, Schijlen, Elio, van de Geest, Henri, Hendriks, Theo, Quillet, MC, Bouwmeester, Harro, Bosch, Dirk, Beekwilder, Jules, "Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory" in Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium (2016):65,
https://hdl.handle.net/21.15107/rcub_ibiss_5441 .

TY  - CONF
AU  - Cankar, Katarina
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - van Houwelingen, Adele
AU  - Schijlen, Elio
AU  - van de Geest, Henri
AU  - Hendriks, Theo
AU  - Quillet, Marie-Christine
AU  - Bouwmeester, Harro
AU  - Bosch, Dirk
AU  - Beekwilder, Jules
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6164
PB  - Vancouver: University of British Columbia
C3  - 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada
T1  - Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory
SP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6164
ER  - 

@conference{
author = "Cankar, Katarina and Bogdanović, Milica and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and van Houwelingen, Adele and Schijlen, Elio and van de Geest, Henri and Hendriks, Theo and Quillet, Marie-Christine and Bouwmeester, Harro and Bosch, Dirk and Beekwilder, Jules",
year = "2015",
publisher = "Vancouver: University of British Columbia",
journal = "12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada",
title = "Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory",
pages = "102",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6164"
}

Cankar, K., Bogdanović, M., Todorović, S., Dragićević, M., Simonović, A., van Houwelingen, A., Schijlen, E., van de Geest, H., Hendriks, T., Quillet, M., Bouwmeester, H., Bosch, D.,& Beekwilder, J.. (2015). Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory. in 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada
Vancouver: University of British Columbia., 102.
https://hdl.handle.net/21.15107/rcub_ibiss_6164

Cankar K, Bogdanović M, Todorović S, Dragićević M, Simonović A, van Houwelingen A, Schijlen E, van de Geest H, Hendriks T, Quillet M, Bouwmeester H, Bosch D, Beekwilder J. Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory. in 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada. 2015;:102.
https://hdl.handle.net/21.15107/rcub_ibiss_6164 .

Cankar, Katarina, Bogdanović, Milica, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, van Houwelingen, Adele, Schijlen, Elio, van de Geest, Henri, Hendriks, Theo, Quillet, Marie-Christine, Bouwmeester, Harro, Bosch, Dirk, Beekwilder, Jules, "Genomic organization and expression of sesquiterpene lactone biosynthetic genes in chicory" in 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids: TERPNET 2015; 2015 Jun 1-5; Vancouver, Canada (2015):102,
https://hdl.handle.net/21.15107/rcub_ibiss_6164 .

TY  - CONF
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Cankar, Katarina
AU  - Beekwilder, Jules
AU  - Bouwmeester, Harro
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6160
AB  - Germacrene A synthase (GAS) catalyzes an important step in guaianolide biosynthesis converting farnesyl pyrophosphate to germacrene A – universal sesquiterpene precursor in chicory. The function of two genes coding for GAS – CiGASlo and CiGASsh, was studied by gene silencing. Chicory was transformed with amiRNA constructs designed to specifically silence either of the two genes, and regenerated transgenic plants were tested for changes in gene expression and sesquiterpene lactones content. Silencing was successful in most of the clones, which had reduced gene expression. However, the amiRNA constructs were not specific for CiGASlo or CiGASsh, so that both genes were silenced in most transgenic plants. To verify that the gene silencing had impact on sesquiterpene lactone levels, three representative guaianolide oxalates (lactucin-15-oxalate, deoxilactucin-15-oxalate and lactucopicrin-15-oxalate) were identified and quantified by UPLC-MS/MS in roots and shoots of transgenic and control plants. Data analyzes showed unequal segregation of targeted compounds in the plant tissues, and implied that the synthesis of these compounds highly depends on the expression of GAS genes in the shoot and that these metabolites are pooled in the shoot, while the root guaianolide oxalates content is regulated on a level different from GAS expression. Nevertheless gene silencing reduced the targeted metabolites levels significantly. It was concluded that both genes are involved in guaianolide biosynthesis, and that their complex pattern of expression in different parts of the plant determines the amount of guaianolides and their derivatives.
PB  - Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Study of chicory germacrene-A synthase function in guaianolide biosynthesis through amiRNA-induced gene silencing
SP  - 20
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6160
ER  - 

@conference{
author = "Dragićević, Milan and Bogdanović, Milica and Cankar, Katarina and Beekwilder, Jules and Bouwmeester, Harro and Todorović, Slađana and Simonović, Ana",
year = "2015",
abstract = "Germacrene A synthase (GAS) catalyzes an important step in guaianolide biosynthesis converting farnesyl pyrophosphate to germacrene A – universal sesquiterpene precursor in chicory. The function of two genes coding for GAS – CiGASlo and CiGASsh, was studied by gene silencing. Chicory was transformed with amiRNA constructs designed to specifically silence either of the two genes, and regenerated transgenic plants were tested for changes in gene expression and sesquiterpene lactones content. Silencing was successful in most of the clones, which had reduced gene expression. However, the amiRNA constructs were not specific for CiGASlo or CiGASsh, so that both genes were silenced in most transgenic plants. To verify that the gene silencing had impact on sesquiterpene lactone levels, three representative guaianolide oxalates (lactucin-15-oxalate, deoxilactucin-15-oxalate and lactucopicrin-15-oxalate) were identified and quantified by UPLC-MS/MS in roots and shoots of transgenic and control plants. Data analyzes showed unequal segregation of targeted compounds in the plant tissues, and implied that the synthesis of these compounds highly depends on the expression of GAS genes in the shoot and that these metabolites are pooled in the shoot, while the root guaianolide oxalates content is regulated on a level different from GAS expression. Nevertheless gene silencing reduced the targeted metabolites levels significantly. It was concluded that both genes are involved in guaianolide biosynthesis, and that their complex pattern of expression in different parts of the plant determines the amount of guaianolides and their derivatives.",
publisher = "Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Study of chicory germacrene-A synthase function in guaianolide biosynthesis through amiRNA-induced gene silencing",
pages = "20-20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6160"
}

Dragićević, M., Bogdanović, M., Cankar, K., Beekwilder, J., Bouwmeester, H., Todorović, S.,& Simonović, A.. (2015). Study of chicory germacrene-A synthase function in guaianolide biosynthesis through amiRNA-induced gene silencing. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”., 20-20.
https://hdl.handle.net/21.15107/rcub_ibiss_6160

Dragićević M, Bogdanović M, Cankar K, Beekwilder J, Bouwmeester H, Todorović S, Simonović A. Study of chicory germacrene-A synthase function in guaianolide biosynthesis through amiRNA-induced gene silencing. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:20-20.
https://hdl.handle.net/21.15107/rcub_ibiss_6160 .

Dragićević, Milan, Bogdanović, Milica, Cankar, Katarina, Beekwilder, Jules, Bouwmeester, Harro, Todorović, Slađana, Simonović, Ana, "Study of chicory germacrene-A synthase function in guaianolide biosynthesis through amiRNA-induced gene silencing" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):20-20,
https://hdl.handle.net/21.15107/rcub_ibiss_6160 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Cankar, Katarina
AU  - Beekwilder, Jules
AU  - Bouwmeester, Harro
AU  - Subotić, Angelina
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6159
AB  - Chicory (Cichorium intybus L.) is rich in sesquiterpene lactones, bitter compounds whose biosynthesis involves several recently characterized genes, namely germacrene A synthase (GAS), in chicory present as long and short form, and germacrene A oxidase (GAO, CYP71AV8). Promoters of these genes have been studied by cloning promoter regions to drive the expression of eGFP, and obtaining stable chicory transformants carrying promoter constructs. Due to incompatibility of eGFP with another fluorescent marker – DsRED, which was used for selection of transformed clones, promoter activity was detected in transgenic plants by RT-PCR and qRT-PCR techniques. Most of the obtained chicory clones were expressing GFP, in roots, leaves, stems and flowers, suggesting that cloned promoters were functional in different chicory organs. The promoters were characterized by different strength - GAO and GAS long promoters were stronger than both GAS short promoters, judging by eGFP expression level. The promoters also showed partial tissue specificity – GAS long was active in roots, leaves, stems and flowers, while GAS short promoters were mainly active in chicory roots.
PB  - Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Germacrene A synthase and oxidase promoter analysis in chicory
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6159
ER  - 

@conference{
author = "Bogdanović, Milica and Dragićević, Milan and Cankar, Katarina and Beekwilder, Jules and Bouwmeester, Harro and Subotić, Angelina and Simonović, Ana and Todorović, Slađana",
year = "2015",
abstract = "Chicory (Cichorium intybus L.) is rich in sesquiterpene lactones, bitter compounds whose biosynthesis involves several recently characterized genes, namely germacrene A synthase (GAS), in chicory present as long and short form, and germacrene A oxidase (GAO, CYP71AV8). Promoters of these genes have been studied by cloning promoter regions to drive the expression of eGFP, and obtaining stable chicory transformants carrying promoter constructs. Due to incompatibility of eGFP with another fluorescent marker – DsRED, which was used for selection of transformed clones, promoter activity was detected in transgenic plants by RT-PCR and qRT-PCR techniques. Most of the obtained chicory clones were expressing GFP, in roots, leaves, stems and flowers, suggesting that cloned promoters were functional in different chicory organs. The promoters were characterized by different strength - GAO and GAS long promoters were stronger than both GAS short promoters, judging by eGFP expression level. The promoters also showed partial tissue specificity – GAS long was active in roots, leaves, stems and flowers, while GAS short promoters were mainly active in chicory roots.",
publisher = "Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Germacrene A synthase and oxidase promoter analysis in chicory",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6159"
}

Bogdanović, M., Dragićević, M., Cankar, K., Beekwilder, J., Bouwmeester, H., Subotić, A., Simonović, A.,& Todorović, S.. (2015). Germacrene A synthase and oxidase promoter analysis in chicory. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”., 19-19.
https://hdl.handle.net/21.15107/rcub_ibiss_6159

Bogdanović M, Dragićević M, Cankar K, Beekwilder J, Bouwmeester H, Subotić A, Simonović A, Todorović S. Germacrene A synthase and oxidase promoter analysis in chicory. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:19-19.
https://hdl.handle.net/21.15107/rcub_ibiss_6159 .

Bogdanović, Milica, Dragićević, Milan, Cankar, Katarina, Beekwilder, Jules, Bouwmeester, Harro, Subotić, Angelina, Simonović, Ana, Todorović, Slađana, "Germacrene A synthase and oxidase promoter analysis in chicory" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):19-19,
https://hdl.handle.net/21.15107/rcub_ibiss_6159 .

TY  - JOUR
AU  - Liu, Qing
AU  - Manzano, David
AU  - Tanić, Nikola
AU  - Pešić, Milica
AU  - Banković, Jasna Z.
AU  - Pateraki, Irini
AU  - Ricard, Lea
AU  - Ferrer, Albert
AU  - de Vos, Ric
AU  - van de Krol, Sander
AU  - Bouwmeester, Harro
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2218
AB  - Parthenolide, the main bioactive compound of the medicinal plant
   feverfew (Tanacetum parthenium), is a promising anti-cancer drug.
   However, the biosynthetic pathway of parthenolide has not been
   elucidated yet. Here we report on the isolation and characterization of
   all the genes from feverfew that are required for the biosynthesis of
   parthenolide, using a combination of 454 sequencing of a feverfew
   glandular trichome cDNA library, co-expression analysis and
   metabolomics. When parthenolide biosynthesis was reconstituted by
   transient co-expression of all pathway genes in Nicotiana benthamiana,
   up to 1.4 mu g g(-1) parthenolide was produced, mostly present as
   cysteine and glutathione conjugates. These relatively polar conjugates
   were highly active against colon cancer cells, with only slightly lower
   activity than free parthenolide. In addition to these biosynthetic
   genes, another gene encoding a costunolide and parthenolide 3
   beta-hydroxylase was identified opening up further options to improve
   the water solubility of parthenolide and therefore its potential as a
   drug. (C) 2014 International Metabolic Engineering Society. Published by
   Elsevier Inc. All rights reserved.
T2  - Metabolic Engineering
T1  - Elucidation and in planta reconstitution of the parthenolide
 biosynthetic pathway
VL  - 23
DO  - 10.1016/j.ymben.2014.03.005
SP  - 145
EP  - 153
ER  - 

@article{
author = "Liu, Qing and Manzano, David and Tanić, Nikola and Pešić, Milica and Banković, Jasna Z. and Pateraki, Irini and Ricard, Lea and Ferrer, Albert and de Vos, Ric and van de Krol, Sander and Bouwmeester, Harro",
year = "2014",
abstract = "Parthenolide, the main bioactive compound of the medicinal plant
   feverfew (Tanacetum parthenium), is a promising anti-cancer drug.
   However, the biosynthetic pathway of parthenolide has not been
   elucidated yet. Here we report on the isolation and characterization of
   all the genes from feverfew that are required for the biosynthesis of
   parthenolide, using a combination of 454 sequencing of a feverfew
   glandular trichome cDNA library, co-expression analysis and
   metabolomics. When parthenolide biosynthesis was reconstituted by
   transient co-expression of all pathway genes in Nicotiana benthamiana,
   up to 1.4 mu g g(-1) parthenolide was produced, mostly present as
   cysteine and glutathione conjugates. These relatively polar conjugates
   were highly active against colon cancer cells, with only slightly lower
   activity than free parthenolide. In addition to these biosynthetic
   genes, another gene encoding a costunolide and parthenolide 3
   beta-hydroxylase was identified opening up further options to improve
   the water solubility of parthenolide and therefore its potential as a
   drug. (C) 2014 International Metabolic Engineering Society. Published by
   Elsevier Inc. All rights reserved.",
journal = "Metabolic Engineering",
title = "Elucidation and in planta reconstitution of the parthenolide
 biosynthetic pathway",
volume = "23",
doi = "10.1016/j.ymben.2014.03.005",
pages = "145-153"
}

Liu, Q., Manzano, D., Tanić, N., Pešić, M., Banković, J. Z., Pateraki, I., Ricard, L., Ferrer, A., de Vos, R., van de Krol, S.,& Bouwmeester, H.. (2014). Elucidation and in planta reconstitution of the parthenolide
 biosynthetic pathway. in Metabolic Engineering, 23, 145-153.
https://doi.org/10.1016/j.ymben.2014.03.005

Liu Q, Manzano D, Tanić N, Pešić M, Banković JZ, Pateraki I, Ricard L, Ferrer A, de Vos R, van de Krol S, Bouwmeester H. Elucidation and in planta reconstitution of the parthenolide
 biosynthetic pathway. in Metabolic Engineering. 2014;23:145-153.
doi:10.1016/j.ymben.2014.03.005 .

Liu, Qing, Manzano, David, Tanić, Nikola, Pešić, Milica, Banković, Jasna Z., Pateraki, Irini, Ricard, Lea, Ferrer, Albert, de Vos, Ric, van de Krol, Sander, Bouwmeester, Harro, "Elucidation and in planta reconstitution of the parthenolide
 biosynthetic pathway" in Metabolic Engineering, 23 (2014):145-153,
https://doi.org/10.1016/j.ymben.2014.03.005 . .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Cankar, Katarina
AU  - Beekwilder, Jules
AU  - Bouwmeester, Harro
AU  - Simonović, Ana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6228
AB  - Chicory (Cichorium intybus L.) is rich in sesquiterpene lactones, compounds known for their bitter taste and medicinal properties. Most enzymes involved in the biosynthetic pathway of these secondary metabolites have recently been discovered and characterized. The first step in their biosynthesis is catalyzed by germacrene A synthase (GAS), in chicory present in two forms - long and short, and several P450 mono-oxygenases. So far, promoters of these genes have not been studied, and little is known about the spatial and temporal regulation of their expression. To address this issue, four vectors for plant transformation containing promoter-reporter gene fusions were designed and constructed by Gateway cloning, including one for GAS long, two for GAS short, and one for the cytochrome P450, germacrene A oxidase. As a marker for co-transformation, DsRED, a red fluorescent protein, was used, while the studied promoters were inserted to drive GFP/GUS fusion, to allow for visualization of promoter activity. Integrity and function of the constructs were checked by agroinfiltration in lettuce (Lactuca sativa Cv. Olof) - a transient transformation assay. Infiltration was performed with Agrobacterium tumefaciens, carrying the promoter constructs. Transformation success was checked five days after infiltration by fluorescent stereomicroscopy, and both DsRED and GFP were detected, indicating that the chicory promoters were active in lettuce. DsRED had strong and uniform fluorescence in all samples, but GFP fluorescence varied among plants infiltrated with different constructs. The GAS long promoter had strongest expression, followed by the P450 and the two rather weak GAS short promoters. The fluorescence was visible only in the infiltrated parts of the leaves, in tissues between leaf veins, but not in the veins themselves. Both abaxial and adaxial leaf sides were fluorescing. There were no differences observed between spatial distribution of DsRED and GFP: all infiltrated parts showed both markers. Since these vectors were confirmed to be functional, stable transformants of chicory will be generated by transformation using A. rhizogenes carrying the same promoter constructs.
PB  - Serbian Plant Physiology Society, Institute for Biological Research "Siniša Stanković", University of Belgrade
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
T1  - Vector construction for promoter analyses in chicory and fluorescence evaluation by agroinfiltration
SP  - 60
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6228
ER  - 

@conference{
author = "Bogdanović, Milica and Todorović, Slađana and Dragićević, Milan and Cankar, Katarina and Beekwilder, Jules and Bouwmeester, Harro and Simonović, Ana",
year = "2013",
abstract = "Chicory (Cichorium intybus L.) is rich in sesquiterpene lactones, compounds known for their bitter taste and medicinal properties. Most enzymes involved in the biosynthetic pathway of these secondary metabolites have recently been discovered and characterized. The first step in their biosynthesis is catalyzed by germacrene A synthase (GAS), in chicory present in two forms - long and short, and several P450 mono-oxygenases. So far, promoters of these genes have not been studied, and little is known about the spatial and temporal regulation of their expression. To address this issue, four vectors for plant transformation containing promoter-reporter gene fusions were designed and constructed by Gateway cloning, including one for GAS long, two for GAS short, and one for the cytochrome P450, germacrene A oxidase. As a marker for co-transformation, DsRED, a red fluorescent protein, was used, while the studied promoters were inserted to drive GFP/GUS fusion, to allow for visualization of promoter activity. Integrity and function of the constructs were checked by agroinfiltration in lettuce (Lactuca sativa Cv. Olof) - a transient transformation assay. Infiltration was performed with Agrobacterium tumefaciens, carrying the promoter constructs. Transformation success was checked five days after infiltration by fluorescent stereomicroscopy, and both DsRED and GFP were detected, indicating that the chicory promoters were active in lettuce. DsRED had strong and uniform fluorescence in all samples, but GFP fluorescence varied among plants infiltrated with different constructs. The GAS long promoter had strongest expression, followed by the P450 and the two rather weak GAS short promoters. The fluorescence was visible only in the infiltrated parts of the leaves, in tissues between leaf veins, but not in the veins themselves. Both abaxial and adaxial leaf sides were fluorescing. There were no differences observed between spatial distribution of DsRED and GFP: all infiltrated parts showed both markers. Since these vectors were confirmed to be functional, stable transformants of chicory will be generated by transformation using A. rhizogenes carrying the same promoter constructs.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade",
title = "Vector construction for promoter analyses in chicory and fluorescence evaluation by agroinfiltration",
pages = "60-61",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6228"
}

Bogdanović, M., Todorović, S., Dragićević, M., Cankar, K., Beekwilder, J., Bouwmeester, H.,& Simonović, A.. (2013). Vector construction for promoter analyses in chicory and fluorescence evaluation by agroinfiltration. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
Serbian Plant Physiology Society, Institute for Biological Research "Siniša Stanković", University of Belgrade., 60-61.
https://hdl.handle.net/21.15107/rcub_ibiss_6228

Bogdanović M, Todorović S, Dragićević M, Cankar K, Beekwilder J, Bouwmeester H, Simonović A. Vector construction for promoter analyses in chicory and fluorescence evaluation by agroinfiltration. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade. 2013;:60-61.
https://hdl.handle.net/21.15107/rcub_ibiss_6228 .

Bogdanović, Milica, Todorović, Slađana, Dragićević, Milan, Cankar, Katarina, Beekwilder, Jules, Bouwmeester, Harro, Simonović, Ana, "Vector construction for promoter analyses in chicory and fluorescence evaluation by agroinfiltration" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade (2013):60-61,
https://hdl.handle.net/21.15107/rcub_ibiss_6228 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Banjanac, Tijana
AU  - Dragićević, Milan
AU  - Verstappen, Francel
AU  - Bouwmeester, Harro
AU  - Simonović, Ana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6226
AB  - Chicory (Cichorium intybus L.) is a plant valued for its medicinal and culinary properties. C. intybus owes its healing effect, at least in part, to a group of secondary metabolites known as sesquiterpene lactones. These compounds are shown to have anti-feedant, anti-fungal, anti-bacterial, anti-protozoan, schistomicidal, molluscicidal and anti-cancer effects. Since they are present in relatively small amounts in the plant, there has been ongoing interest in ways to improve the production and yield of these compounds. One way of achieving this goal is Agrobacterium-mediated transformation, which is known to boost secondary metabolism. Chicory is readily transformed with A. rhizogenes to produce hairy-roots, from which whole plants can be regenerated. Several clones previously obtained through A. rhizogenes A4 transformation were analyzed by targeted LC-MS for the content of 8-deoxylactucin, lactucin, dihydrolactucin and lactupicrin. Three clones and one untransformed plant were chosen for the analysis, based on growth, biomass production and initial screening for these metabolites. Both leaves and roots of in vitro grown plants were analysed, in two growth phases: non-flowering rosette and flowering elongated plant. It was found that the selected clones indeed produce more sesquiterpene lactones than the control plants, and that this was dependent on the plant organ and growth phase. Roots in general contain more of these compounds than leaves, and roots of flowering clones have higher content of all target sesquiterpene lactones than control plants. Since these plants were transformed with the native Ri plasmid (pRiA4-GUS), expression of bacterial genes - rolA, rolB and rolC, which are known to affect secondary metabolism, was quantified, in an attempt to correlate elevated compound content with the rol genes’ transcript levels. Expression was highest for the rolC, and barely measurable for rolB. Higher transcript level of these genes was detected in roots, and all three clones had high expression of rolC in the roots of flowering plants, which coincides with sesquiterpene lactones presence.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
T1  - Sesquiterpene lactone production in transformed chicory
SP  - 93
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6226
ER  - 

@conference{
author = "Bogdanović, Milica and Todorović, Slađana and Banjanac, Tijana and Dragićević, Milan and Verstappen, Francel and Bouwmeester, Harro and Simonović, Ana",
year = "2013",
abstract = "Chicory (Cichorium intybus L.) is a plant valued for its medicinal and culinary properties. C. intybus owes its healing effect, at least in part, to a group of secondary metabolites known as sesquiterpene lactones. These compounds are shown to have anti-feedant, anti-fungal, anti-bacterial, anti-protozoan, schistomicidal, molluscicidal and anti-cancer effects. Since they are present in relatively small amounts in the plant, there has been ongoing interest in ways to improve the production and yield of these compounds. One way of achieving this goal is Agrobacterium-mediated transformation, which is known to boost secondary metabolism. Chicory is readily transformed with A. rhizogenes to produce hairy-roots, from which whole plants can be regenerated. Several clones previously obtained through A. rhizogenes A4 transformation were analyzed by targeted LC-MS for the content of 8-deoxylactucin, lactucin, dihydrolactucin and lactupicrin. Three clones and one untransformed plant were chosen for the analysis, based on growth, biomass production and initial screening for these metabolites. Both leaves and roots of in vitro grown plants were analysed, in two growth phases: non-flowering rosette and flowering elongated plant. It was found that the selected clones indeed produce more sesquiterpene lactones than the control plants, and that this was dependent on the plant organ and growth phase. Roots in general contain more of these compounds than leaves, and roots of flowering clones have higher content of all target sesquiterpene lactones than control plants. Since these plants were transformed with the native Ri plasmid (pRiA4-GUS), expression of bacterial genes - rolA, rolB and rolC, which are known to affect secondary metabolism, was quantified, in an attempt to correlate elevated compound content with the rol genes’ transcript levels. Expression was highest for the rolC, and barely measurable for rolB. Higher transcript level of these genes was detected in roots, and all three clones had high expression of rolC in the roots of flowering plants, which coincides with sesquiterpene lactones presence.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade",
title = "Sesquiterpene lactone production in transformed chicory",
pages = "93-94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6226"
}

Bogdanović, M., Todorović, S., Banjanac, T., Dragićević, M., Verstappen, F., Bouwmeester, H.,& Simonović, A.. (2013). Sesquiterpene lactone production in transformed chicory. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
Belgrade: Serbian Plant Physiology Society., 93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_6226

Bogdanović M, Todorović S, Banjanac T, Dragićević M, Verstappen F, Bouwmeester H, Simonović A. Sesquiterpene lactone production in transformed chicory. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade. 2013;:93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_6226 .

Bogdanović, Milica, Todorović, Slađana, Banjanac, Tijana, Dragićević, Milan, Verstappen, Francel, Bouwmeester, Harro, Simonović, Ana, "Sesquiterpene lactone production in transformed chicory" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade (2013):93-94,
https://hdl.handle.net/21.15107/rcub_ibiss_6226 .

TY  - JOUR
AU  - Majdi, Mohammad
AU  - Liu, Qing
AU  - Karimzadeh, Ghasem
AU  - Malboobi, Mohammad Ali
AU  - Beekwilder, Jules
AU  - Cankar, Katarina
AU  - de Vos, Ric
AU  - Todorović, Slađana
AU  - Simonović, Ana
AU  - Bouwmeester, Harro
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1257
AB  - Feverfew (Tanacetum parthenium) is a perennial medicinal herb and is a rich source of sesquiterpene lactones. Parthenolide is the main sesquiterpene lactone in feverfew and has attracted attention because of its medicinal potential for treatment of migraine and cancer. In the present work the parthenolide content in different tissues and developmental stages of feverfew was analyzed to study the timing and localization of parthenolide biosynthesis. The strongest accumulating tissue was subsequently used to isolate sesquiterpene synthases with the goal to isolate the gene encoding the first dedicated step in parthenolide biosynthesis. This led to the isolation and charachterization of a germacrene A synthase (TpGAS) and an (E)-beta-caryophyllene synthase (TpCarS). Transcript level patterns of both sesquiterpene synthases were analyzed in different tissues and glandular trichomes. Although TpGAS was expressed in all aerial tissues, the highest expression was observed in tissues that contain high concentrations of parthenolide and in flowers the highest expression was observed in the biosynthetically most active stages of flower development. The high expression of TpGAS in glandular trichomes which also contain the highest concentration of parthenolide, suggests that glandular trichomes are the secretory tissues where parthenolide biosynthesis and accumulation occur. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Phytochemistry
T1  - Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip.)
IS  - 14-15
VL  - 72
EP  - 1750
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1257
ER  - 

@article{
author = "Majdi, Mohammad and Liu, Qing and Karimzadeh, Ghasem and Malboobi, Mohammad Ali and Beekwilder, Jules and Cankar, Katarina and de Vos, Ric and Todorović, Slađana and Simonović, Ana and Bouwmeester, Harro",
year = "2011",
abstract = "Feverfew (Tanacetum parthenium) is a perennial medicinal herb and is a rich source of sesquiterpene lactones. Parthenolide is the main sesquiterpene lactone in feverfew and has attracted attention because of its medicinal potential for treatment of migraine and cancer. In the present work the parthenolide content in different tissues and developmental stages of feverfew was analyzed to study the timing and localization of parthenolide biosynthesis. The strongest accumulating tissue was subsequently used to isolate sesquiterpene synthases with the goal to isolate the gene encoding the first dedicated step in parthenolide biosynthesis. This led to the isolation and charachterization of a germacrene A synthase (TpGAS) and an (E)-beta-caryophyllene synthase (TpCarS). Transcript level patterns of both sesquiterpene synthases were analyzed in different tissues and glandular trichomes. Although TpGAS was expressed in all aerial tissues, the highest expression was observed in tissues that contain high concentrations of parthenolide and in flowers the highest expression was observed in the biosynthetically most active stages of flower development. The high expression of TpGAS in glandular trichomes which also contain the highest concentration of parthenolide, suggests that glandular trichomes are the secretory tissues where parthenolide biosynthesis and accumulation occur. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Phytochemistry",
title = "Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip.)",
number = "14-15",
volume = "72",
pages = "1750",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1257"
}

Majdi, M., Liu, Q., Karimzadeh, G., Malboobi, M. A., Beekwilder, J., Cankar, K., de Vos, R., Todorović, S., Simonović, A.,& Bouwmeester, H.. (2011). Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip.). in Phytochemistry, 72(14-15).
https://hdl.handle.net/21.15107/rcub_ibiss_1257

Majdi M, Liu Q, Karimzadeh G, Malboobi MA, Beekwilder J, Cankar K, de Vos R, Todorović S, Simonović A, Bouwmeester H. Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip.). in Phytochemistry. 2011;72(14-15):null-1750.
https://hdl.handle.net/21.15107/rcub_ibiss_1257 .

Majdi, Mohammad, Liu, Qing, Karimzadeh, Ghasem, Malboobi, Mohammad Ali, Beekwilder, Jules, Cankar, Katarina, de Vos, Ric, Todorović, Slađana, Simonović, Ana, Bouwmeester, Harro, "Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip.)" in Phytochemistry, 72, no. 14-15 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1257 .