TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jevremović, Slađana
AU  - Petrović, Zoran Lj.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5415
AB  - In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins
PB  - Greifswald: Leibniz Institute for Plasma Science and Technology
C3  - Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
T1  - Changing the plant tissue morphology and physiology by plasma treatment
SP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5415
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Živković, Suzana and Milutinović, Milica and Jevremović, Slađana and Petrović, Zoran Lj.",
year = "2021",
abstract = "In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins",
publisher = "Greifswald: Leibniz Institute for Plasma Science and Technology",
journal = "Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany",
title = "Changing the plant tissue morphology and physiology by plasma treatment",
pages = "14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5415"
}

Puač, N., Škoro, N., Živković, S., Milutinović, M., Jevremović, S.,& Petrović, Z. Lj.. (2021). Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
Greifswald: Leibniz Institute for Plasma Science and Technology., 14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415

Puač N, Škoro N, Živković S, Milutinović M, Jevremović S, Petrović ZL. Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany. 2021;:14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

Puač, Nevena, Škoro, Nikola, Živković, Suzana, Milutinović, Milica, Jevremović, Slađana, Petrović, Zoran Lj., "Changing the plant tissue morphology and physiology by plasma treatment" in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany (2021):14,
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

TY  - JOUR
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ristić, Mihailo
AU  - Filipović, Biljana
AU  - Milutinović, Milica
AU  - Perišić, Mirjana
AU  - Puač, Nevena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4394
AB  - When exposed to stressful conditions, plants produce numerous volatile organic compounds (VOCs) that have different biological and environmental functions. VOCs emitted during the rehydration process by the fronds of desiccation tolerant fern Asplenium ceterach L. were investigated. Headspace GC–MS analysis revealed that the volatiles profile of rustyback fern is mainly composed of fatty acid derivatives: isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols, and related compounds. Aerial parts of the rustyback fern do not contain monoterpene-type, sesquiterpene-type, and diterpene-type hydrocarbons or corresponding terpenoids. Online detection of VOCs using proton-transfer reaction mass spectrometry (PTR–MS) showed a significant increase in emission intensity of dominant volatiles during the first hours of the rehydration process. Twelve hours after re-watering, emission of detected volatiles had returned to the basal levels that corresponded to hydrated plants. During the early phase of rehydration malondialdehyde (MDA) content in fronds, as an indicator of membrane damage, decreased rapidly which implies that lipoxygenase activity is not stimulated during the recovery process of rustyback fern.
PB  - Basel: MDPI
T2  - Biology
T1  - Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds
IS  - 7
VL  - 10
DO  - 10.3390/biology10070574
SP  - 574
ER  - 

@article{
author = "Živković, Suzana and Skorić, Marijana and Ristić, Mihailo and Filipović, Biljana and Milutinović, Milica and Perišić, Mirjana and Puač, Nevena",
year = "2021",
abstract = "When exposed to stressful conditions, plants produce numerous volatile organic compounds (VOCs) that have different biological and environmental functions. VOCs emitted during the rehydration process by the fronds of desiccation tolerant fern Asplenium ceterach L. were investigated. Headspace GC–MS analysis revealed that the volatiles profile of rustyback fern is mainly composed of fatty acid derivatives: isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols, and related compounds. Aerial parts of the rustyback fern do not contain monoterpene-type, sesquiterpene-type, and diterpene-type hydrocarbons or corresponding terpenoids. Online detection of VOCs using proton-transfer reaction mass spectrometry (PTR–MS) showed a significant increase in emission intensity of dominant volatiles during the first hours of the rehydration process. Twelve hours after re-watering, emission of detected volatiles had returned to the basal levels that corresponded to hydrated plants. During the early phase of rehydration malondialdehyde (MDA) content in fronds, as an indicator of membrane damage, decreased rapidly which implies that lipoxygenase activity is not stimulated during the recovery process of rustyback fern.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds",
number = "7",
volume = "10",
doi = "10.3390/biology10070574",
pages = "574"
}

Živković, S., Skorić, M., Ristić, M., Filipović, B., Milutinović, M., Perišić, M.,& Puač, N.. (2021). Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds. in Biology
Basel: MDPI., 10(7), 574.
https://doi.org/10.3390/biology10070574

Živković S, Skorić M, Ristić M, Filipović B, Milutinović M, Perišić M, Puač N. Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds. in Biology. 2021;10(7):574.
doi:10.3390/biology10070574 .

Živković, Suzana, Skorić, Marijana, Ristić, Mihailo, Filipović, Biljana, Milutinović, Milica, Perišić, Mirjana, Puač, Nevena, "Rehydration Process in Rustyback Fern (Asplenium ceterach L.): Profiling of Volatile Organic Compounds" in Biology, 10, no. 7 (2021):574,
https://doi.org/10.3390/biology10070574 . .

TY  - CONF
AU  - Živković, Suzana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4396
AB  - Recent scientific, technological and methodological advances in the fields of plasma physics and plant
physiology offer a wide range of possibilities for employment of plasma technology in treatment of
plants. Application of PAW or direct plasma treatment represent an alternative and valuable approach
to plant biotechnology being chemically-free, energy efficient, and environmentally friendly. However,
the distinct mechanisms responsible for the positive effects of plasma treatment on plant cells are
still vague. Plant calli cultures (calli grown aseptically on semisolid agar medium) and calli suspension
cultures (calli grown aseptically in liquid medium in test tubes, shake flasks, or bioreactors) with or
without addition of plant growth regulators are used to illustrate changes in physiological response of
plant cells/tissues induced by plasma treatment. Key challenges and obstacles to achieve precise and
localized in vivo treatments of living cells and tissues will be discussed in light of current knowledge in
the field.
PB  - Sarajevo: University of East Sarajevo
PB  - Zvornik: Faculty of Technology
C3  - I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina
T1  - Plasma treatment of plants: puzzle games for biologists and physicists
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4396
ER  - 

@conference{
author = "Živković, Suzana",
year = "2021",
abstract = "Recent scientific, technological and methodological advances in the fields of plasma physics and plant
physiology offer a wide range of possibilities for employment of plasma technology in treatment of
plants. Application of PAW or direct plasma treatment represent an alternative and valuable approach
to plant biotechnology being chemically-free, energy efficient, and environmentally friendly. However,
the distinct mechanisms responsible for the positive effects of plasma treatment on plant cells are
still vague. Plant calli cultures (calli grown aseptically on semisolid agar medium) and calli suspension
cultures (calli grown aseptically in liquid medium in test tubes, shake flasks, or bioreactors) with or
without addition of plant growth regulators are used to illustrate changes in physiological response of
plant cells/tissues induced by plasma treatment. Key challenges and obstacles to achieve precise and
localized in vivo treatments of living cells and tissues will be discussed in light of current knowledge in
the field.",
publisher = "Sarajevo: University of East Sarajevo, Zvornik: Faculty of Technology",
journal = "I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina",
title = "Plasma treatment of plants: puzzle games for biologists and physicists",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4396"
}

Živković, S.. (2021). Plasma treatment of plants: puzzle games for biologists and physicists. in I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina
Sarajevo: University of East Sarajevo., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_4396

Živković S. Plasma treatment of plants: puzzle games for biologists and physicists. in I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina. 2021;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_4396 .

Živković, Suzana, "Plasma treatment of plants: puzzle games for biologists and physicists" in I Training School: “Plasma applications for smart and sustainable agriculture”– PlAgri; 2021 Mar 17-19; Jahorina, Nosnia and Herzegovina (2021):8,
https://hdl.handle.net/21.15107/rcub_ibiss_4396 .

TY  - JOUR
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Maksimović, Vuk
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Božunović, Jelena
AU  - Banjanac, Tijana
AU  - Mišić, Danijela
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3699
AB  - Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH• and ABTS•+), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(–)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.
PB  - Elsevier B.V.
T2  - South African Journal of Botany
T1  - Antioxidant and antimicrobial activity of two Asplenium species
VL  - 132
DO  - 10.1016/j.sajb.2020.03.034
SP  - 180
EP  - 187
ER  - 

@article{
author = "Živković, Suzana and Milutinović, Milica and Maksimović, Vuk and Ćirić, Ana and Ivanov, Marija and Božunović, Jelena and Banjanac, Tijana and Mišić, Danijela",
year = "2020",
abstract = "Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH• and ABTS•+), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(–)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.",
publisher = "Elsevier B.V.",
journal = "South African Journal of Botany",
title = "Antioxidant and antimicrobial activity of two Asplenium species",
volume = "132",
doi = "10.1016/j.sajb.2020.03.034",
pages = "180-187"
}

Živković, S., Milutinović, M., Maksimović, V., Ćirić, A., Ivanov, M., Božunović, J., Banjanac, T.,& Mišić, D.. (2020). Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany
Elsevier B.V.., 132, 180-187.
https://doi.org/10.1016/j.sajb.2020.03.034

Živković S, Milutinović M, Maksimović V, Ćirić A, Ivanov M, Božunović J, Banjanac T, Mišić D. Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany. 2020;132:180-187.
doi:10.1016/j.sajb.2020.03.034 .

Živković, Suzana, Milutinović, Milica, Maksimović, Vuk, Ćirić, Ana, Ivanov, Marija, Božunović, Jelena, Banjanac, Tijana, Mišić, Danijela, "Antioxidant and antimicrobial activity of two Asplenium species" in South African Journal of Botany, 132 (2020):180-187,
https://doi.org/10.1016/j.sajb.2020.03.034 . .

TY  - JOUR
AU  - Bradu, Corina
AU  - Kutasi, Kinga
AU  - Magureanu, Monica
AU  - Puač, Nevena
AU  - Živković, Suzana
PY  - 2020
UR  - https://iopscience.iop.org/article/10.1088/1361-6463/ab795a
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3679
AB  - The interest in plasma-activated water (PAW) has been greatly increasing during the last few years due to its potential applications in biological and medical sciences, as well as in agriculture and food industry. The effect of PAW is caused by the reactive oxygen and nitrogen species (RONS) generated in the liquid exposed to plasma. This work reviews recent research on the formation of reactive nitrogen species (RNS) in aqueous solutions treated by non-thermal plasma. The most important chemical reactions leading to the production of these active species in the gas and liquid phase, as well as their chemistry in the liquid, correlated with their lifetime are addressed. The literature data on the most important long-lived RNS in PAW (nitrite and nitrate) and the short-lived ones (peroxynitrite and peroxynitrate) are reviewed. The reported results show that their concentrations strongly depend on the type of electrical discharge, gas composition, liquid properties and treatment conditions, and thus tuning the PAW composition over a relatively wide range can be achieved. Results on the possible application of PAW in agriculture are also reviewed. The role of RNS in this area is related to their participation in various signalling pathways in plants, which regulate metabolic processes, plant development, response to stress, etc, and thus can finally lead to enhanced germination and/or faster germination process and increase in plant growth.
PB  - IOP Publishing
T2  - Journal of Physics D: Applied Physics
T1  - Reactive nitrogen species in plasma-activated water: generation, chemistry and application in agriculture
IS  - 22
VL  - 53
DO  - 10.1088/1361-6463/AB795A
SP  - 223001
ER  - 

@article{
author = "Bradu, Corina and Kutasi, Kinga and Magureanu, Monica and Puač, Nevena and Živković, Suzana",
year = "2020",
abstract = "The interest in plasma-activated water (PAW) has been greatly increasing during the last few years due to its potential applications in biological and medical sciences, as well as in agriculture and food industry. The effect of PAW is caused by the reactive oxygen and nitrogen species (RONS) generated in the liquid exposed to plasma. This work reviews recent research on the formation of reactive nitrogen species (RNS) in aqueous solutions treated by non-thermal plasma. The most important chemical reactions leading to the production of these active species in the gas and liquid phase, as well as their chemistry in the liquid, correlated with their lifetime are addressed. The literature data on the most important long-lived RNS in PAW (nitrite and nitrate) and the short-lived ones (peroxynitrite and peroxynitrate) are reviewed. The reported results show that their concentrations strongly depend on the type of electrical discharge, gas composition, liquid properties and treatment conditions, and thus tuning the PAW composition over a relatively wide range can be achieved. Results on the possible application of PAW in agriculture are also reviewed. The role of RNS in this area is related to their participation in various signalling pathways in plants, which regulate metabolic processes, plant development, response to stress, etc, and thus can finally lead to enhanced germination and/or faster germination process and increase in plant growth.",
publisher = "IOP Publishing",
journal = "Journal of Physics D: Applied Physics",
title = "Reactive nitrogen species in plasma-activated water: generation, chemistry and application in agriculture",
number = "22",
volume = "53",
doi = "10.1088/1361-6463/AB795A",
pages = "223001"
}

Bradu, C., Kutasi, K., Magureanu, M., Puač, N.,& Živković, S.. (2020). Reactive nitrogen species in plasma-activated water: generation, chemistry and application in agriculture. in Journal of Physics D: Applied Physics
IOP Publishing., 53(22), 223001.
https://doi.org/10.1088/1361-6463/AB795A

Bradu C, Kutasi K, Magureanu M, Puač N, Živković S. Reactive nitrogen species in plasma-activated water: generation, chemistry and application in agriculture. in Journal of Physics D: Applied Physics. 2020;53(22):223001.
doi:10.1088/1361-6463/AB795A .

Bradu, Corina, Kutasi, Kinga, Magureanu, Monica, Puač, Nevena, Živković, Suzana, "Reactive nitrogen species in plasma-activated water: generation, chemistry and application in agriculture" in Journal of Physics D: Applied Physics, 53, no. 22 (2020):223001,
https://doi.org/10.1088/1361-6463/AB795A . .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Maletić, Dejan
AU  - Živković, Suzana
AU  - Selaković, Nenad
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2019
UR  - http://neon.dpp.fmph.uniba.sk/sappxxii/download/SAPP_XXII_JSPP_XI_Book_of_Contributed_Papers.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6199
AB  - Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.
PB  - Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava
C3  - Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
T1  - Diagnostics of atmospheric pressure plasmas and their application in agriculture
SP  - 68
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6199
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Maletić, Dejan and Živković, Suzana and Selaković, Nenad and Malović, Gordana and Petrović, Zoran Lj",
year = "2019",
abstract = "Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.",
publisher = "Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava",
journal = "Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia",
title = "Diagnostics of atmospheric pressure plasmas and their application in agriculture",
pages = "68-69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6199"
}

Puač, N., Škoro, N., Maletić, D., Živković, S., Selaković, N., Malović, G.,& Petrović, Z. L.. (2019). Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava., 68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199

Puač N, Škoro N, Maletić D, Živković S, Selaković N, Malović G, Petrović ZL. Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia. 2019;:68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .

Puač, Nevena, Škoro, Nikola, Maletić, Dejan, Živković, Suzana, Selaković, Nenad, Malović, Gordana, Petrović, Zoran Lj, "Diagnostics of atmospheric pressure plasmas and their application in agriculture" in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia (2019):68-69,
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Mišić, Danijela
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161719300525?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3342
AB  - Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
T2  - Journal of Plant Physiology
T1  - Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
VL  - 237
DO  - 10.1016/j.jplph.2019.04.006
SP  - 87
EP  - 94
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Mišić, Danijela",
year = "2019",
abstract = "Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.",
journal = "Journal of Plant Physiology",
title = "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.",
volume = "237",
doi = "10.1016/j.jplph.2019.04.006",
pages = "87-94"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D.,& Mišić, D.. (2019). Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology, 237, 87-94.
https://doi.org/10.1016/j.jplph.2019.04.006

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Mišić D. Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology. 2019;237:87-94.
doi:10.1016/j.jplph.2019.04.006 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Mišić, Danijela, "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh." in Journal of Plant Physiology, 237 (2019):87-94,
https://doi.org/10.1016/j.jplph.2019.04.006 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Petrović, Zoran Lj
AU  - Škoro, Nikola
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4398
AB  - The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.
PB  - Heron Press
C3  - Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
T1  - Alteration in plant tissue morphology and physiology induced by plasma treatment
SP  - 63
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4398
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Milutinović, Milica and Puač, Nevena and Petrović, Zoran Lj and Škoro, Nikola",
year = "2019",
abstract = "The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.",
publisher = "Heron Press",
journal = "Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)",
title = "Alteration in plant tissue morphology and physiology induced by plasma treatment",
pages = "63-64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4398"
}

Živković, S., Jevremović, S., Milutinović, M., Puač, N., Petrović, Z. L.,& Škoro, N.. (2019). Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
Heron Press., 63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398

Živković S, Jevremović S, Milutinović M, Puač N, Petrović ZL, Škoro N. Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019). 2019;:63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

Živković, Suzana, Jevremović, Slađana, Milutinović, Milica, Puač, Nevena, Petrović, Zoran Lj, Škoro, Nikola, "Alteration in plant tissue morphology and physiology induced by plasma treatment" in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019) (2019):63-64,
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

TY  - CONF
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perišić, Mirjana
AU  - Mišić, Danijela
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4293
AB  - Phosphinothricin, active ingredient of the commercial herbicide BASTA, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation, which leads to the elevation of ammonium levels in plants and further to various physiological alternations, ammonium toxicity and lethality. Nepeta rtanjensis Diklić & Milojević essential oil (NrEO), rich in iridoid monoterpenoids nepetalactones, has been previously highlighted as a potential bioherbicide inducing oxidative stress in model plants. Interestingly, simultaneous foliar application of NrEO and BASTA, two agents showing differential mode of herbicidal action, suspends BASTA-induced toxicity in Arabidopsis thaliana plants by maintaining sub-toxic and/or sub-lethal ammonium concentration in tissues. NrEO effectively reduces BASTA efficacy, and the degree of antagonism between these agents escalates at increasing BASTA and NrEO levels. Regulation of GS activity, as influenced by BASTA, NrEO, and their joint action, partially occurs at transcriptional, posttranscriptional, and/or posttranslational levels, and is organ-specific. Furthermore, BASTA and NrEO interaction mitigates the effects of these agents, applied independently, on chlorophyll, soluble sugars and organic acids metabolism. Results suggest the existence of complex regulatory mechanisms determining antagonistic BASTA and NrEO interaction, and highlight the possible applications of the BASTA/NrEO mixture in agricultural practice. Furthermore, the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils, is suggested here as a environment-friendly approach for weed control.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4293
ER  - 

@conference{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perišić, Mirjana and Mišić, Danijela",
year = "2018",
abstract = "Phosphinothricin, active ingredient of the commercial herbicide BASTA, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation, which leads to the elevation of ammonium levels in plants and further to various physiological alternations, ammonium toxicity and lethality. Nepeta rtanjensis Diklić & Milojević essential oil (NrEO), rich in iridoid monoterpenoids nepetalactones, has been previously highlighted as a potential bioherbicide inducing oxidative stress in model plants. Interestingly, simultaneous foliar application of NrEO and BASTA, two agents showing differential mode of herbicidal action, suspends BASTA-induced toxicity in Arabidopsis thaliana plants by maintaining sub-toxic and/or sub-lethal ammonium concentration in tissues. NrEO effectively reduces BASTA efficacy, and the degree of antagonism between these agents escalates at increasing BASTA and NrEO levels. Regulation of GS activity, as influenced by BASTA, NrEO, and their joint action, partially occurs at transcriptional, posttranscriptional, and/or posttranslational levels, and is organ-specific. Furthermore, BASTA and NrEO interaction mitigates the effects of these agents, applied independently, on chlorophyll, soluble sugars and organic acids metabolism. Results suggest the existence of complex regulatory mechanisms determining antagonistic BASTA and NrEO interaction, and highlight the possible applications of the BASTA/NrEO mixture in agricultural practice. Furthermore, the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils, is suggested here as a environment-friendly approach for weed control.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4293"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perišić, M.,& Mišić, D.. (2018). Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 49.
https://hdl.handle.net/21.15107/rcub_ibiss_4293

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perišić M, Mišić D. Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:49.
https://hdl.handle.net/21.15107/rcub_ibiss_4293 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perišić, Mirjana, Mišić, Danijela, "Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):49,
https://hdl.handle.net/21.15107/rcub_ibiss_4293 .

TY  - JOUR
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4395
AB  - In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low-pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment conditions.
PB  - Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA
T2  - Plasma Processes and Polymers
T1  - Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water
IS  - 2
VL  - 15
DO  - 10.1002/ppap.201700082
SP  - e1700082
ER  - 

@article{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Petrović, Zoran Lj",
year = "2018",
abstract = "In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low-pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment conditions.",
publisher = "Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA",
journal = "Plasma Processes and Polymers",
title = "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water",
number = "2",
volume = "15",
doi = "10.1002/ppap.201700082",
pages = "e1700082"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Malović, G.,& Petrović, Z. L.. (2018). Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers
Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA., 15(2), e1700082.
https://doi.org/10.1002/ppap.201700082

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Malović G, Petrović ZL. Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers. 2018;15(2):e1700082.
doi:10.1002/ppap.201700082 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Petrović, Zoran Lj, "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water" in Plasma Processes and Polymers, 15, no. 2 (2018):e1700082,
https://doi.org/10.1002/ppap.201700082 . .

TY  - JOUR
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2018
UR  - http://doi.wiley.com/10.1002/ppap.201700082
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3538
AB  - In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low‐pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment
T2  - Plasma Processes and Polymers
T1  - Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water
IS  - 2
VL  - 15
DO  - 10.1002/ppap.201700082
SP  - 1700082
ER  - 

@article{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Petrović, Zoran Lj.",
year = "2018",
abstract = "In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low‐pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment",
journal = "Plasma Processes and Polymers",
title = "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water",
number = "2",
volume = "15",
doi = "10.1002/ppap.201700082",
pages = "1700082"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Malović, G.,& Petrović, Z. Lj.. (2018). Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers, 15(2), 1700082.
https://doi.org/10.1002/ppap.201700082

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Malović G, Petrović ZL. Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers. 2018;15(2):1700082.
doi:10.1002/ppap.201700082 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Petrović, Zoran Lj., "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water" in Plasma Processes and Polymers, 15, no. 2 (2018):1700082,
https://doi.org/10.1002/ppap.201700082 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Petrović, Zoran Lj
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4397
AB  - Plant callus of three different and phylogenetically distinct types of plants-carrot (Daucus carrota L.), and two ornamental plant species: horned pansy (Viola cornuta L. ‛Lutea Splendens’) and small bearded iris (Iris reichenbachii Heuff.) was treated using plasma needle. Plasma treatment induced somatic embryogenesis in carrot calli cultured on basal medium and stimulated growth of both carrot calli and transgenic calli of horned pansy. Surface changes of nonregenerative globular calli of iris after plasma treatment were followed by enhanced accumulation of arabinogalactan proteins in epidermal cells and around the globule. Our findings indicate that the plasma treatment could be considered as useful and promising technique for induction of embryogenesis, as well as for regeneration and multiplication of transgenic plants.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications
T1  - Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues
IS  - WS2-5
VL  - 2
SP  - 646
EP  - 649
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4397
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Puač, Nevena and Škoro, Nikola and Petrović, Zoran Lj",
year = "2018",
abstract = "Plant callus of three different and phylogenetically distinct types of plants-carrot (Daucus carrota L.), and two ornamental plant species: horned pansy (Viola cornuta L. ‛Lutea Splendens’) and small bearded iris (Iris reichenbachii Heuff.) was treated using plasma needle. Plasma treatment induced somatic embryogenesis in carrot calli cultured on basal medium and stimulated growth of both carrot calli and transgenic calli of horned pansy. Surface changes of nonregenerative globular calli of iris after plasma treatment were followed by enhanced accumulation of arabinogalactan proteins in epidermal cells and around the globule. Our findings indicate that the plasma treatment could be considered as useful and promising technique for induction of embryogenesis, as well as for regeneration and multiplication of transgenic plants.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications",
title = "Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues",
number = "WS2-5",
volume = "2",
pages = "646-649",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4397"
}

Živković, S., Jevremović, S., Puač, N., Škoro, N.,& Petrović, Z. L.. (2018). Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues. in Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications
Belgrade: Serbian Academy of Sciences and Arts., 2(WS2-5), 646-649.
https://hdl.handle.net/21.15107/rcub_ibiss_4397

Živković S, Jevremović S, Puač N, Škoro N, Petrović ZL. Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues. in Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications. 2018;2(WS2-5):646-649.
https://hdl.handle.net/21.15107/rcub_ibiss_4397 .

Živković, Suzana, Jevremović, Slađana, Puač, Nevena, Škoro, Nikola, Petrović, Zoran Lj, "Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues" in Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications, 2, no. WS2-5 (2018):646-649,
https://hdl.handle.net/21.15107/rcub_ibiss_4397 .

TY  - JOUR
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Krstić Milošević, Dijana
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2018
UR  - http://link.springer.com/10.1140/epjd/e2017-80329-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3030
AB  - Today’s reality is connected with mitigation of threats from the new chemical and biological warfare agents. A novel investigation of cold plasmas in contact with liquids presented in this paper demonstrated that the chemically reactive environment produced by atmospheric pressure plasma jet (APPJ) is potentially capable of rapid destruction of chemical warfare agents in a broad spectrum. The decontamination of three different chemical warfare agent surrogates dissolved in liquid is investigated by using an easily transportable APPJ. The jet is powered by a kHz signal source connected to a low-voltage DC source and with He as working gas. The detailed investigation of electrical properties is performed for various plasmas at different distances from the sample. The measurements of plasma properties in situ are supported by the optical spectrometry measurements, whereas the high performance liquid chromatography measurements before and after the treatment of aqueous solutions of Malathion, Fenitrothion and Dimethyl Methylphosphonate. These solutions are used to evaluate destruction and its efficiency for specific neural agent simulants. The particular removal rates are found to be from 56% up to 96% during 10 min treatment. The data obtained provide basis to evaluate APPJ’s efficiency at different operating conditions. The presented results are promising and could be improved with different operating conditions and optimization of the decontamination process.
T2  - The European Physical Journal D
T1  - Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet
IS  - 1
VL  - 72
DO  - 10.1140/epjd/e2017-80329-9
SP  - 2
ER  - 

@article{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Krstić Milošević, Dijana and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran Lj.",
year = "2018",
abstract = "Today’s reality is connected with mitigation of threats from the new chemical and biological warfare agents. A novel investigation of cold plasmas in contact with liquids presented in this paper demonstrated that the chemically reactive environment produced by atmospheric pressure plasma jet (APPJ) is potentially capable of rapid destruction of chemical warfare agents in a broad spectrum. The decontamination of three different chemical warfare agent surrogates dissolved in liquid is investigated by using an easily transportable APPJ. The jet is powered by a kHz signal source connected to a low-voltage DC source and with He as working gas. The detailed investigation of electrical properties is performed for various plasmas at different distances from the sample. The measurements of plasma properties in situ are supported by the optical spectrometry measurements, whereas the high performance liquid chromatography measurements before and after the treatment of aqueous solutions of Malathion, Fenitrothion and Dimethyl Methylphosphonate. These solutions are used to evaluate destruction and its efficiency for specific neural agent simulants. The particular removal rates are found to be from 56% up to 96% during 10 min treatment. The data obtained provide basis to evaluate APPJ’s efficiency at different operating conditions. The presented results are promising and could be improved with different operating conditions and optimization of the decontamination process.",
journal = "The European Physical Journal D",
title = "Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet",
number = "1",
volume = "72",
doi = "10.1140/epjd/e2017-80329-9",
pages = "2"
}

Škoro, N., Puač, N., Živković, S., Krstić Milošević, D., Cvelbar, U., Malović, G.,& Petrović, Z. Lj.. (2018). Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet. in The European Physical Journal D, 72(1), 2.
https://doi.org/10.1140/epjd/e2017-80329-9

Škoro N, Puač N, Živković S, Krstić Milošević D, Cvelbar U, Malović G, Petrović ZL. Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet. in The European Physical Journal D. 2018;72(1):2.
doi:10.1140/epjd/e2017-80329-9 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Krstić Milošević, Dijana, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran Lj., "Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet" in The European Physical Journal D, 72, no. 1 (2018):2,
https://doi.org/10.1140/epjd/e2017-80329-9 . .

TY  - CONF
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6222
AB  - Non-equilibrium atmospheric pressure plasmas (APP) are chemically active media with properties adjustable to comply with various application demands. ln biomedical field, so far APP have demonstrated excellent applicability in sterilization and cleaning, i.e. for treatment of organic materials and microorganisms. Atmospheric pressure plasma jets (APPJ) proved to be technically simple and efficient sources of cold APP which are suitable for different application setups. Recently, a new research field-plasmas in agriculture emerged, widening number of applications. Since APPJs can also operate in contact with liquids, they are suitable for new applications in plasma agriculture. Initial investigations show that chemically reactive environment produced by these sources can influence and modify physical and chemical properties of liquids. As one of the main surface water contaminants are pesticides used in agriculture our idea is to use APP for water detoxification. Namely, we conducted a study on decontamination of water samples polluted with different pesticides, i.e. organophosphate compounds, by using APPJs. We used two different jet configurations powered by a kHz signal source with He as working gas and treated liquid samples at different distances between the jet and the sample surface, for different duration times and for different liquid sample volumes. Optical and electrical characterization of the APPJs is performed in order to obtain information about the plasma properties and stability of the treatment conditions. Liquid sample analysis before and after the treatment are executed by using high performance liquid chromatography (HPLC) and liquid chromatography coupled with mass spectrometry (LC-MS) in order to follow degradation of organophosphates. Significant and efficient degradation of both pesticides is noticed and appearance of degradation products is observed in the liquid sample. Dependence of the decontamination efficiency on treatment time is determined. We also investigated toxicity of degradation products. Thus, from the data obtained we could assess the decontamination efficiency for different APPJ types and also to provide information about mechanisms governing the plasma destruction of organophosphates.
PB  - Zagreb: Institute of Physics
C3  - Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia
T1  - Use of atmospheric pressure plasmas for decontamination of water containing organophosphates
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6222
ER  - 

@conference{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran",
year = "2017",
abstract = "Non-equilibrium atmospheric pressure plasmas (APP) are chemically active media with properties adjustable to comply with various application demands. ln biomedical field, so far APP have demonstrated excellent applicability in sterilization and cleaning, i.e. for treatment of organic materials and microorganisms. Atmospheric pressure plasma jets (APPJ) proved to be technically simple and efficient sources of cold APP which are suitable for different application setups. Recently, a new research field-plasmas in agriculture emerged, widening number of applications. Since APPJs can also operate in contact with liquids, they are suitable for new applications in plasma agriculture. Initial investigations show that chemically reactive environment produced by these sources can influence and modify physical and chemical properties of liquids. As one of the main surface water contaminants are pesticides used in agriculture our idea is to use APP for water detoxification. Namely, we conducted a study on decontamination of water samples polluted with different pesticides, i.e. organophosphate compounds, by using APPJs. We used two different jet configurations powered by a kHz signal source with He as working gas and treated liquid samples at different distances between the jet and the sample surface, for different duration times and for different liquid sample volumes. Optical and electrical characterization of the APPJs is performed in order to obtain information about the plasma properties and stability of the treatment conditions. Liquid sample analysis before and after the treatment are executed by using high performance liquid chromatography (HPLC) and liquid chromatography coupled with mass spectrometry (LC-MS) in order to follow degradation of organophosphates. Significant and efficient degradation of both pesticides is noticed and appearance of degradation products is observed in the liquid sample. Dependence of the decontamination efficiency on treatment time is determined. We also investigated toxicity of degradation products. Thus, from the data obtained we could assess the decontamination efficiency for different APPJ types and also to provide information about mechanisms governing the plasma destruction of organophosphates.",
publisher = "Zagreb: Institute of Physics",
journal = "Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia",
title = "Use of atmospheric pressure plasmas for decontamination of water containing organophosphates",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6222"
}

Škoro, N., Puač, N., Živković, S., Cvelbar, U., Malović, G.,& Petrović, Z.. (2017). Use of atmospheric pressure plasmas for decontamination of water containing organophosphates. in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia
Zagreb: Institute of Physics., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_6222

Škoro N, Puač N, Živković S, Cvelbar U, Malović G, Petrović Z. Use of atmospheric pressure plasmas for decontamination of water containing organophosphates. in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia. 2017;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_6222 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran, "Use of atmospheric pressure plasmas for decontamination of water containing organophosphates" in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia (2017):28,
https://hdl.handle.net/21.15107/rcub_ibiss_6222 .

TY  - CONF
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Mišić, Danijela
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6221
AB  - Nonequilibrium atmospheric pressure plasma proved to produce reactive chemistry environment at gas temperatures as low as room temperature which is perfect for treatments of different kind of surfaces. Over the past several years cold atmospheric pressure plasma applications in surface sterilization and cleaning have been extensively studied. Recently, the research has been directed to utilization of this type of plasma far agriculture. Plasma is used directly for treatment of seeds, but, also, indirectly through plasma activated water. In the case of decontamination of water contaminated with conventional pesticides and insecticides plasma can play important role. One of suitable plasma sources for these treatments are atmospheric pressure plasma jets (APPJ), since they are small-size (easily scalable to larger systems), relatively technically simple and cost-effective devices. In our case, we investigated treatments of water contaminated with three types of pesticides. Decontamination is achieved by applying the jet on the liquid surface generating high-energy electrons, reactive oxygen and nitrogen species, radicals and ultraviolet radiation. Reactive species created al the gas-liquid interface effectively degrade organic pollutants in the water. However, due to the complexity of the system, key mechanisms important for destruction of particular contaminant are not clearly known. Moreover, optimisation of the system is recondite by reciprocal influence of the jet and surface. Thus, we have performed detailed and systematic investigation of the APPJ-liquid sample system properties at different conditions and far different pollutants dissolved in water. Electrical and optical measurements provided information about the discharge running at different powers and distances from the sample. We could relate electrical characteristics with spatial profiles of the discharge. Moreover, optical spectrometry provided data on emission lines characteristic far the plasma used far decontamination. By using liquid chromatography measurements of contaminated water samples before and after the treatment we acquired evidence on destruction of specific pollutants and found decontamination rates. AII this data is valuable far evaluation of APPJ efficiency at different operating conditions and optimisation of the decontamination process.
PB  - lnstitute of Plasma Physics CAS
C3  - Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic
T1  - Application of atmospheric pressure plasmas in agriculture for wastewater cleaning
SP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6221
ER  - 

@conference{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Mišić, Danijela and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran",
year = "2017",
abstract = "Nonequilibrium atmospheric pressure plasma proved to produce reactive chemistry environment at gas temperatures as low as room temperature which is perfect for treatments of different kind of surfaces. Over the past several years cold atmospheric pressure plasma applications in surface sterilization and cleaning have been extensively studied. Recently, the research has been directed to utilization of this type of plasma far agriculture. Plasma is used directly for treatment of seeds, but, also, indirectly through plasma activated water. In the case of decontamination of water contaminated with conventional pesticides and insecticides plasma can play important role. One of suitable plasma sources for these treatments are atmospheric pressure plasma jets (APPJ), since they are small-size (easily scalable to larger systems), relatively technically simple and cost-effective devices. In our case, we investigated treatments of water contaminated with three types of pesticides. Decontamination is achieved by applying the jet on the liquid surface generating high-energy electrons, reactive oxygen and nitrogen species, radicals and ultraviolet radiation. Reactive species created al the gas-liquid interface effectively degrade organic pollutants in the water. However, due to the complexity of the system, key mechanisms important for destruction of particular contaminant are not clearly known. Moreover, optimisation of the system is recondite by reciprocal influence of the jet and surface. Thus, we have performed detailed and systematic investigation of the APPJ-liquid sample system properties at different conditions and far different pollutants dissolved in water. Electrical and optical measurements provided information about the discharge running at different powers and distances from the sample. We could relate electrical characteristics with spatial profiles of the discharge. Moreover, optical spectrometry provided data on emission lines characteristic far the plasma used far decontamination. By using liquid chromatography measurements of contaminated water samples before and after the treatment we acquired evidence on destruction of specific pollutants and found decontamination rates. AII this data is valuable far evaluation of APPJ efficiency at different operating conditions and optimisation of the decontamination process.",
publisher = "lnstitute of Plasma Physics CAS",
journal = "Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic",
title = "Application of atmospheric pressure plasmas in agriculture for wastewater cleaning",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6221"
}

Škoro, N., Puač, N., Živković, S., Mišić, D., Cvelbar, U., Malović, G.,& Petrović, Z.. (2017). Application of atmospheric pressure plasmas in agriculture for wastewater cleaning. in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic
lnstitute of Plasma Physics CAS., 13.
https://hdl.handle.net/21.15107/rcub_ibiss_6221

Škoro N, Puač N, Živković S, Mišić D, Cvelbar U, Malović G, Petrović Z. Application of atmospheric pressure plasmas in agriculture for wastewater cleaning. in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic. 2017;:13.
https://hdl.handle.net/21.15107/rcub_ibiss_6221 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Mišić, Danijela, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran, "Application of atmospheric pressure plasmas in agriculture for wastewater cleaning" in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic (2017):13,
https://hdl.handle.net/21.15107/rcub_ibiss_6221 .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Šašić, Vuk
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6198
AB  - In this abstract we report on influence of direct and indirect plasma treatments on catalase enzyme activity in Paulownia tomentosa seeds. The direct treatment of the seeds was performed in low-pressure RF plasma system for different treatment times. After treatments these seeds were imbibed with distilled water. The other set of P. tomentosa seeds was imbibed with plasma activated water (PAW). PAW was produced by using atmospheric pressure plasma source in treatments with different durations. Seeds from both sets were exposed to the same conditions and after 5 days activity of catalase enzyme was measured. In comparison to the control sample, differences in the activity was observed both regarding direct and PAW treated seeds and regarding duration of treatments.
PB  - Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa
C3  - Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal
T1  - Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6198
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Šašić, Vuk and Malović, Gordana and Petrović, Zoran Lj",
year = "2017",
abstract = "In this abstract we report on influence of direct and indirect plasma treatments on catalase enzyme activity in Paulownia tomentosa seeds. The direct treatment of the seeds was performed in low-pressure RF plasma system for different treatment times. After treatments these seeds were imbibed with distilled water. The other set of P. tomentosa seeds was imbibed with plasma activated water (PAW). PAW was produced by using atmospheric pressure plasma source in treatments with different durations. Seeds from both sets were exposed to the same conditions and after 5 days activity of catalase enzyme was measured. In comparison to the control sample, differences in the activity was observed both regarding direct and PAW treated seeds and regarding duration of treatments.",
publisher = "Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa",
journal = "Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal",
title = "Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6198"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Šašić, V., Malović, G.,& Petrović, Z. L.. (2017). Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water. in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal
Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa., 180.
https://hdl.handle.net/21.15107/rcub_ibiss_6198

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Šašić V, Malović G, Petrović ZL. Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water. in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal. 2017;:180.
https://hdl.handle.net/21.15107/rcub_ibiss_6198 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Šašić, Vuk, Malović, Gordana, Petrović, Zoran Lj, "Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water" in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal (2017):180,
https://hdl.handle.net/21.15107/rcub_ibiss_6198 .

TY  - CONF
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ristić, Mihailo
AU  - Perišić, Mirjana
AU  - Puač, Nevena
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6190
AB  - Volatile organic compounds (VOC) emitted by fronds of desiccation tolerant fern Asplenium ceterach L. during the rehydration process were investigated. Head-space GC-MS analysis was used in order to determine the volatile compounds in dry and rehydrated sample of golden fern. It was found that the VOC profile of golden fern is based mainly on isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols and related compounds. Aerial parts of fresh and dry fronds do not contain monoterpene-, sesquiterpene- and diterpene-type hydrocarbons or corresponding terpenoids. In order to determine the composition of the VOC during the process of rehydration, we have applied proton-transfer reaction mass spectrometry (PTR-MS). PTR-MS is a sensitive technique that allows real-time detection of VOCs emitted from plants. We have used dry plants to establish a base line and then distilled water was added to induce hydration process of the golden fern plant. Masses in the range from m/z 21 to m/z 300 were measured with dwell time of 200 ms and for the time period of 24 hours. The experiment was repeated for five plants. PTR-MS measurements revealed that the amounts of compounds, determined by head-space GC-MS, generally exhibit a significant increase after addition of water. After about 20 hours the values measured by PTR-MS for these masses are reduced to a new base line that corresponds to the rehydrated plant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6190
ER  - 

@conference{
author = "Živković, Suzana and Skorić, Marijana and Ristić, Mihailo and Perišić, Mirjana and Puač, Nevena and Malović, Gordana and Petrović, Zoran Lj",
year = "2015",
abstract = "Volatile organic compounds (VOC) emitted by fronds of desiccation tolerant fern Asplenium ceterach L. during the rehydration process were investigated. Head-space GC-MS analysis was used in order to determine the volatile compounds in dry and rehydrated sample of golden fern. It was found that the VOC profile of golden fern is based mainly on isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols and related compounds. Aerial parts of fresh and dry fronds do not contain monoterpene-, sesquiterpene- and diterpene-type hydrocarbons or corresponding terpenoids. In order to determine the composition of the VOC during the process of rehydration, we have applied proton-transfer reaction mass spectrometry (PTR-MS). PTR-MS is a sensitive technique that allows real-time detection of VOCs emitted from plants. We have used dry plants to establish a base line and then distilled water was added to induce hydration process of the golden fern plant. Masses in the range from m/z 21 to m/z 300 were measured with dwell time of 200 ms and for the time period of 24 hours. The experiment was repeated for five plants. PTR-MS measurements revealed that the amounts of compounds, determined by head-space GC-MS, generally exhibit a significant increase after addition of water. After about 20 hours the values measured by PTR-MS for these masses are reduced to a new base line that corresponds to the rehydrated plant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration",
pages = "187",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6190"
}

Živković, S., Skorić, M., Ristić, M., Perišić, M., Puač, N., Malović, G.,& Petrović, Z. L.. (2015). Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 187.
https://hdl.handle.net/21.15107/rcub_ibiss_6190

Živković S, Skorić M, Ristić M, Perišić M, Puač N, Malović G, Petrović ZL. Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:187.
https://hdl.handle.net/21.15107/rcub_ibiss_6190 .

Živković, Suzana, Skorić, Marijana, Ristić, Mihailo, Perišić, Mirjana, Puač, Nevena, Malović, Gordana, Petrović, Zoran Lj, "Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):187,
https://hdl.handle.net/21.15107/rcub_ibiss_6190 .

TY  - CONF
AU  - Dmitrović, Slavica
AU  - Perišić, Mirjana
AU  - Stojić, Andreja
AU  - Živković, Suzana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Aničić, Neda
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6185
AB  - The possibility of using essential oil (EO) of Nepeta rtanjensis and N. cataria as bioherbicides against Ambrosia artemisiifolia L., an important weed and allergen species, was explored. In vitro culture experimental system of A. artemisiifolia has been established and ragweed shoots were exposed to the atmosphere enriched with volatile compounds (VOCs) emitted from EOs. N. rtanjensis EO was characterized by high amounts of 4aα,7α,7aβ-nepetalactone (trans,cis-nepetalactone), while N. cataria EO possessed high amounts of 4aα,7α,7aα-nepetalactone (cis,trans-nepetalactone). Reduction in A. artemisiifolia shoots growth and their discoloration, in the presence of N. rtanjensis and N. cataria EOs (2% and 4%, final nepetalactone concentrations) was observed after 2 weeks of exposure. An antioxidative defense system of A. artemisiifolia was activated by increasing peroxidases activity or by inactivating catalases and superoxide dismutases activity. Overall, stronger inhibitory effect on shoot growth, CAT activity, and stimulating POX activity was observed for N. cataria EO. N. rtanjensis EO was more efficient in inhibiting rooting and root growth, and in suppressing SOD activity. Therefore, EOs of N. rtanjensis and N. cataria might be recommended as potential bioherbicides against highly allergenic
and weed species A. artemisiifolia. Further exploration of EOs’ mode of action, confirmation of phytotoxicity in field conditions, and feasibility assessment for the commercial production and application in agricultural practice, is the course of our further work.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils
SP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6185
ER  - 

@conference{
author = "Dmitrović, Slavica and Perišić, Mirjana and Stojić, Andreja and Živković, Suzana and Božunović, Jelena and Nestorović Živković, Jasmina and Aničić, Neda and Ristić, Mihailo and Mišić, Danijela",
year = "2015",
abstract = "The possibility of using essential oil (EO) of Nepeta rtanjensis and N. cataria as bioherbicides against Ambrosia artemisiifolia L., an important weed and allergen species, was explored. In vitro culture experimental system of A. artemisiifolia has been established and ragweed shoots were exposed to the atmosphere enriched with volatile compounds (VOCs) emitted from EOs. N. rtanjensis EO was characterized by high amounts of 4aα,7α,7aβ-nepetalactone (trans,cis-nepetalactone), while N. cataria EO possessed high amounts of 4aα,7α,7aα-nepetalactone (cis,trans-nepetalactone). Reduction in A. artemisiifolia shoots growth and their discoloration, in the presence of N. rtanjensis and N. cataria EOs (2% and 4%, final nepetalactone concentrations) was observed after 2 weeks of exposure. An antioxidative defense system of A. artemisiifolia was activated by increasing peroxidases activity or by inactivating catalases and superoxide dismutases activity. Overall, stronger inhibitory effect on shoot growth, CAT activity, and stimulating POX activity was observed for N. cataria EO. N. rtanjensis EO was more efficient in inhibiting rooting and root growth, and in suppressing SOD activity. Therefore, EOs of N. rtanjensis and N. cataria might be recommended as potential bioherbicides against highly allergenic
and weed species A. artemisiifolia. Further exploration of EOs’ mode of action, confirmation of phytotoxicity in field conditions, and feasibility assessment for the commercial production and application in agricultural practice, is the course of our further work.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils",
pages = "159",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6185"
}

Dmitrović, S., Perišić, M., Stojić, A., Živković, S., Božunović, J., Nestorović Živković, J., Aničić, N., Ristić, M.,& Mišić, D.. (2015). The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 159.
https://hdl.handle.net/21.15107/rcub_ibiss_6185

Dmitrović S, Perišić M, Stojić A, Živković S, Božunović J, Nestorović Živković J, Aničić N, Ristić M, Mišić D. The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:159.
https://hdl.handle.net/21.15107/rcub_ibiss_6185 .

Dmitrović, Slavica, Perišić, Mirjana, Stojić, Andreja, Živković, Suzana, Božunović, Jelena, Nestorović Živković, Jasmina, Aničić, Neda, Ristić, Mihailo, Mišić, Danijela, "The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):159,
https://hdl.handle.net/21.15107/rcub_ibiss_6185 .

TY  - CONF
AU  - Skorić, Marijana
AU  - Todorović, Slađana
AU  - Ristić, Mihailo
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Živković, Suzana
AU  - Stojić, Andreja
AU  - Puač, Nevena
AU  - Kanellis, Angelos K
PY  - 2013
UR  - http://terpnet2013.pharm.auth.gr/site/TERPNET_BOOK_OF_ABSTRACTS.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6320
AB  - Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.
PB  - Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki
C3  - TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
T1  - In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6320
ER  - 

@conference{
author = "Skorić, Marijana and Todorović, Slađana and Ristić, Mihailo and Soković, Marina and Glamočlija, Jasmina and Živković, Suzana and Stojić, Andreja and Puač, Nevena and Kanellis, Angelos K",
year = "2013",
abstract = "Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.",
publisher = "Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki",
journal = "TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece",
title = "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds",
pages = "132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6320"
}

Skorić, M., Todorović, S., Ristić, M., Soković, M., Glamočlija, J., Živković, S., Stojić, A., Puač, N.,& Kanellis, A. K.. (2013). In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki., 132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320

Skorić M, Todorović S, Ristić M, Soković M, Glamočlija J, Živković S, Stojić A, Puač N, Kanellis AK. In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece. 2013;:132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .

Skorić, Marijana, Todorović, Slađana, Ristić, Mihailo, Soković, Marina, Glamočlija, Jasmina, Živković, Suzana, Stojić, Andreja, Puač, Nevena, Kanellis, Angelos K, "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds" in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece (2013):132,
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .

TY  - CONF
AU  - Selaković, Nenad
AU  - Jevremović, Slađana
AU  - Živković, Suzana
AU  - Maletić, Dejan
AU  - Puač, Nevena
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6396
AB  - The effects of atmospheric pressure plasmas on living cells and tissues have been studied on numerous occasions in recent literature. lt appears that plasma treatment may find many biomedical applications. There are many types of plasmas that can be generated under ambient pressure and temperature conditions. Plas­ma needle is one of the atmospheric pressure devices that meet the requirements of precise and localized treatment necessary for treatment of plant cells. We have investigated the effect of atmospheric pressure plasma generated by plasma needle device, under ambient pressure and temperature conditions, on carrot somatic embryogenesis. The embryogenic calli cul­tures of carrot (Daucus carrota) were established from storage root explants and maintained on Murashige and Skoog medium (MS) supplemented with 2,4-dichlorphenoxyacetic acid (2,4-D, 1.0 mg L·1) and cultured in dark. Calli samples (10-30 mg) were placed in 96 wells micro-titer plate before the tip of the plasma needle was placed at the edge of the well when plasma was covering whole surface of the samples. The treatment times were 1 O, 30, 60 and 120 s at two powers, namely 0.4 W and 1.4 W. After plasma treatment, calli samples were cultured on basal MS medium without plant growth regulators or on MS medium supplemented with 2,4-D or 2,4-D and kinetin (1.0 mg L-1, each) and cultured in dark for six weeks. Calli number, fresh weight in­crease and developmental stage of formed somatic embryos were estimated. lt was shown that plasma treat­ment notably stimulated growth and somatic embryo formation of calli cultured on basal MS medium. The highest fresh weight increase (-40 fold) was observed after 30 s of plasma needle treatment at 0.4 W. The number and developmental stage of formed somatic embryos depended on duration and power of plasma treatment, as well as the type of culture media. The highest number of somatic embryos was observed when calli samples were grown on basal MS medium after 60 s of plasma treatment at 1.4 W. Furthermore, plasma treatment significantly increased the number of formed somatic embryos on MS media supplemented with plant growth regulators. Our results show that plasma strongly affects growth and somatic embryo forma­tion and development of carrot calli.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6396
ER  - 

@conference{
author = "Selaković, Nenad and Jevremović, Slađana and Živković, Suzana and Maletić, Dejan and Puač, Nevena and Malović, Gordana and Petrović, Zoran Lj",
year = "2013",
abstract = "The effects of atmospheric pressure plasmas on living cells and tissues have been studied on numerous occasions in recent literature. lt appears that plasma treatment may find many biomedical applications. There are many types of plasmas that can be generated under ambient pressure and temperature conditions. Plas­ma needle is one of the atmospheric pressure devices that meet the requirements of precise and localized treatment necessary for treatment of plant cells. We have investigated the effect of atmospheric pressure plasma generated by plasma needle device, under ambient pressure and temperature conditions, on carrot somatic embryogenesis. The embryogenic calli cul­tures of carrot (Daucus carrota) were established from storage root explants and maintained on Murashige and Skoog medium (MS) supplemented with 2,4-dichlorphenoxyacetic acid (2,4-D, 1.0 mg L·1) and cultured in dark. Calli samples (10-30 mg) were placed in 96 wells micro-titer plate before the tip of the plasma needle was placed at the edge of the well when plasma was covering whole surface of the samples. The treatment times were 1 O, 30, 60 and 120 s at two powers, namely 0.4 W and 1.4 W. After plasma treatment, calli samples were cultured on basal MS medium without plant growth regulators or on MS medium supplemented with 2,4-D or 2,4-D and kinetin (1.0 mg L-1, each) and cultured in dark for six weeks. Calli number, fresh weight in­crease and developmental stage of formed somatic embryos were estimated. lt was shown that plasma treat­ment notably stimulated growth and somatic embryo formation of calli cultured on basal MS medium. The highest fresh weight increase (-40 fold) was observed after 30 s of plasma needle treatment at 0.4 W. The number and developmental stage of formed somatic embryos depended on duration and power of plasma treatment, as well as the type of culture media. The highest number of somatic embryos was observed when calli samples were grown on basal MS medium after 60 s of plasma treatment at 1.4 W. Furthermore, plasma treatment significantly increased the number of formed somatic embryos on MS media supplemented with plant growth regulators. Our results show that plasma strongly affects growth and somatic embryo forma­tion and development of carrot calli.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6396"
}

Selaković, N., Jevremović, S., Živković, S., Maletić, D., Puač, N., Malović, G.,& Petrović, Z. L.. (2013). The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota). in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 36.
https://hdl.handle.net/21.15107/rcub_ibiss_6396

Selaković N, Jevremović S, Živković S, Maletić D, Puač N, Malović G, Petrović ZL. The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota). in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:36.
https://hdl.handle.net/21.15107/rcub_ibiss_6396 .

Selaković, Nenad, Jevremović, Slađana, Živković, Suzana, Maletić, Dejan, Puač, Nevena, Malović, Gordana, Petrović, Zoran Lj, "The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):36,
https://hdl.handle.net/21.15107/rcub_ibiss_6396 .

TY  - CONF
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Jovanović, Vladan
AU  - Šiler, Branislav
AU  - Ristić, Mihailo
AU  - Cvetković, Jelena
AU  - Živković, Suzana
AU  - Mišić, Danijela
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6397
AB  - Allelopathy, the chemical mechanism of plant interference, was detected in many plants from fam. Lamiaceae, including Nepeta species. The majority of Nepeta species are characterized by the presence of ne­petalactone as the dominant component in essential oils. This monoterpenoid lactone exists in the form of one or more stereoisomers, and corresponding enantiomers. The present study investigates the allelopathic potential of Nepeta rtanjensís Diklić & Milojević and Nepeta ca­taria L. essential oils which differ in their qualitative nepetalactone content, on the seed germination of four selected crop species (Lepidium sativum L., Lactuca sativa L. cv. Majska Kraljica, Lotus corniculatus L., cv. Bokor and Brassica napus L. cv. napus). GC-MS analysis revealed that trans,cis- stereoisomer of nepetalactone is dom­inant in essential oil of N. rtanjensis (72,03%). Essential oil of N. cataria used in the present study is character­ized by the high content of cís, trans-nepetalactone (90%). Both essential oils exhibited significant allelopath­ic potential against all model species, with N. rtanjensís essential oil being more efficient. These results sug­gest that nepetalactone stereochemistry significantly determines its allelopathic potential, and that the ef­fect of both nepetalactone stereoisomers is dose-dependent. Essential oils reduced seed germination by in­fluencing germination dynamics, or by irreversibly inhibiting the germination of seeds. Among tested crops, L. sativa was shown to be the most sensitive to allelopathic potential of N. rtanjensis and N. cataria essential oils, while B. napus was the most tolerant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6397
ER  - 

@conference{
author = "Nestorović Živković, Jasmina and Dmitrović, Slavica and Jovanović, Vladan and Šiler, Branislav and Ristić, Mihailo and Cvetković, Jelena and Živković, Suzana and Mišić, Danijela",
year = "2013",
abstract = "Allelopathy, the chemical mechanism of plant interference, was detected in many plants from fam. Lamiaceae, including Nepeta species. The majority of Nepeta species are characterized by the presence of ne­petalactone as the dominant component in essential oils. This monoterpenoid lactone exists in the form of one or more stereoisomers, and corresponding enantiomers. The present study investigates the allelopathic potential of Nepeta rtanjensís Diklić & Milojević and Nepeta ca­taria L. essential oils which differ in their qualitative nepetalactone content, on the seed germination of four selected crop species (Lepidium sativum L., Lactuca sativa L. cv. Majska Kraljica, Lotus corniculatus L., cv. Bokor and Brassica napus L. cv. napus). GC-MS analysis revealed that trans,cis- stereoisomer of nepetalactone is dom­inant in essential oil of N. rtanjensis (72,03%). Essential oil of N. cataria used in the present study is character­ized by the high content of cís, trans-nepetalactone (90%). Both essential oils exhibited significant allelopath­ic potential against all model species, with N. rtanjensís essential oil being more efficient. These results sug­gest that nepetalactone stereochemistry significantly determines its allelopathic potential, and that the ef­fect of both nepetalactone stereoisomers is dose-dependent. Essential oils reduced seed germination by in­fluencing germination dynamics, or by irreversibly inhibiting the germination of seeds. Among tested crops, L. sativa was shown to be the most sensitive to allelopathic potential of N. rtanjensis and N. cataria essential oils, while B. napus was the most tolerant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6397"
}

Nestorović Živković, J., Dmitrović, S., Jovanović, V., Šiler, B., Ristić, M., Cvetković, J., Živković, S.,& Mišić, D.. (2013). Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_6397

Nestorović Živković J, Dmitrović S, Jovanović V, Šiler B, Ristić M, Cvetković J, Živković S, Mišić D. Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_6397 .

Nestorović Živković, Jasmina, Dmitrović, Slavica, Jovanović, Vladan, Šiler, Branislav, Ristić, Mihailo, Cvetković, Jelena, Živković, Suzana, Mišić, Danijela, "Allelopathic potential of Nepeta rtanjensis Diklić & Milojević and Nepeta cataria L. essential oils on selected crop species" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):81,
https://hdl.handle.net/21.15107/rcub_ibiss_6397 .

TY  - JOUR
AU  - Radović, Miloš
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Živković, Suzana
AU  - Nikolić, Miloš
AU  - Soković, Marina
AU  - Mišić, Danijela
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5446
AB  - With regard to world’s increasing demand for biologically active compounds, a novel source of xanthones and secoiridoid glycosides has been studied. Zeltnera beyrichii (Torr. & A. Gray) Mans., an
insufficiently acknowledged North American medicinal plant species, may be considered a pharmacological substitute for commercial C. erythraea Rafn, since it accumulates in aerial parts nearly the same amount of secoiridoid glycosides: swertiamarin, gentiopicrin, and sweroside (13.76, 7.56, and 0.17 mmol per 100 g dry weight, respectively) in plants grown under greenhouse condition, and a considerable amount of xanthones: decussatin and eustomin. Additionally, Z. beyrichii produced as much biomass during cultivation in RITA® temporary immersion bioreactors as greenhouse-grown plants, in a third of the time. Plants grown in bioreactors contained moderate levels of total phenolics and total flavonoids, and possessed modest antioxidant activity and antimicrobial potential against eight bacterial and eight fungal species. Therefore, this species may be highly recommended for cultivation either in natural environment, or in bioreactors under in vitro conditions, for producing compounds of interest of modern pharmacology and food industry.
PB  - ACG Publications
T2  - Records of Natural Products
T1  - Bioreactor cultivation of Zeltnera beyrichii (Torr. & A. Gray) Mans.: A novel source of biologically active compounds
IS  - 4
VL  - 7
SP  - 266
EP  - 280
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5446
ER  - 

@article{
author = "Radović, Miloš and Šiler, Branislav and Nestorović Živković, Jasmina and Banjanac, Tijana and Živković, Suzana and Nikolić, Miloš and Soković, Marina and Mišić, Danijela",
year = "2013",
abstract = "With regard to world’s increasing demand for biologically active compounds, a novel source of xanthones and secoiridoid glycosides has been studied. Zeltnera beyrichii (Torr. & A. Gray) Mans., an
insufficiently acknowledged North American medicinal plant species, may be considered a pharmacological substitute for commercial C. erythraea Rafn, since it accumulates in aerial parts nearly the same amount of secoiridoid glycosides: swertiamarin, gentiopicrin, and sweroside (13.76, 7.56, and 0.17 mmol per 100 g dry weight, respectively) in plants grown under greenhouse condition, and a considerable amount of xanthones: decussatin and eustomin. Additionally, Z. beyrichii produced as much biomass during cultivation in RITA® temporary immersion bioreactors as greenhouse-grown plants, in a third of the time. Plants grown in bioreactors contained moderate levels of total phenolics and total flavonoids, and possessed modest antioxidant activity and antimicrobial potential against eight bacterial and eight fungal species. Therefore, this species may be highly recommended for cultivation either in natural environment, or in bioreactors under in vitro conditions, for producing compounds of interest of modern pharmacology and food industry.",
publisher = "ACG Publications",
journal = "Records of Natural Products",
title = "Bioreactor cultivation of Zeltnera beyrichii (Torr. & A. Gray) Mans.: A novel source of biologically active compounds",
number = "4",
volume = "7",
pages = "266-280",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5446"
}

Radović, M., Šiler, B., Nestorović Živković, J., Banjanac, T., Živković, S., Nikolić, M., Soković, M.,& Mišić, D.. (2013). Bioreactor cultivation of Zeltnera beyrichii (Torr. & A. Gray) Mans.: A novel source of biologically active compounds. in Records of Natural Products
ACG Publications., 7(4), 266-280.
https://hdl.handle.net/21.15107/rcub_ibiss_5446

Radović M, Šiler B, Nestorović Živković J, Banjanac T, Živković S, Nikolić M, Soković M, Mišić D. Bioreactor cultivation of Zeltnera beyrichii (Torr. & A. Gray) Mans.: A novel source of biologically active compounds. in Records of Natural Products. 2013;7(4):266-280.
https://hdl.handle.net/21.15107/rcub_ibiss_5446 .

Radović, Miloš, Šiler, Branislav, Nestorović Živković, Jasmina, Banjanac, Tijana, Živković, Suzana, Nikolić, Miloš, Soković, Marina, Mišić, Danijela, "Bioreactor cultivation of Zeltnera beyrichii (Torr. & A. Gray) Mans.: A novel source of biologically active compounds" in Records of Natural Products, 7, no. 4 (2013):266-280,
https://hdl.handle.net/21.15107/rcub_ibiss_5446 .