TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Jevremović, Slađana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6713
AB  - Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.
PB  - COST Action CA21157 COPYTREE
C3  - Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
T1  - Recent advances in somatic embryogenesis induction in Aesculus species
SP  - 38
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Jevremović, Slađana",
year = "2024",
abstract = "Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.",
publisher = "COST Action CA21157 COPYTREE",
journal = "Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia",
title = "Recent advances in somatic embryogenesis induction in Aesculus species",
pages = "38"
}

Zdravković-Korać, S., Ćalić, D., Milojević, J., Belić, M.,& Jevremović, S.. (2024). Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
COST Action CA21157 COPYTREE., 38.

Zdravković-Korać S, Ćalić D, Milojević J, Belić M, Jevremović S. Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia. 2024;:38..

Zdravković-Korać, Snežana, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Jevremović, Slađana, "Recent advances in somatic embryogenesis induction in Aesculus species" in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia (2024):38.

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Ristić-Đurović, Jasna L.
AU  - Ćirković, Saša
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Stanišić, Mariana
AU  - Zdravković-Korać, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6420
AB  - Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions
PB  - Basel: MDPI
T2  - Agriculture
T1  - Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields
IS  - 11
VL  - 13
DO  - 10.3390/agriculture13112120
SP  - 2120
ER  - 

@article{
author = "Ćalić, Dušica and Ristić-Đurović, Jasna L. and Ćirković, Saša and Milojević, Jelena and Belić, Maja and Stanišić, Mariana and Zdravković-Korać, Snežana",
year = "2023",
abstract = "Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields",
number = "11",
volume = "13",
doi = "10.3390/agriculture13112120",
pages = "2120"
}

Ćalić, D., Ristić-Đurović, J. L., Ćirković, S., Milojević, J., Belić, M., Stanišić, M.,& Zdravković-Korać, S.. (2023). Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture
Basel: MDPI., 13(11), 2120.
https://doi.org/10.3390/agriculture13112120

Ćalić D, Ristić-Đurović JL, Ćirković S, Milojević J, Belić M, Stanišić M, Zdravković-Korać S. Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture. 2023;13(11):2120.
doi:10.3390/agriculture13112120 .

Ćalić, Dušica, Ristić-Đurović, Jasna L., Ćirković, Saša, Milojević, Jelena, Belić, Maja, Stanišić, Mariana, Zdravković-Korać, Snežana, "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields" in Agriculture, 13, no. 11 (2023):2120,
https://doi.org/10.3390/agriculture13112120 . .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6090
AB  - A spinach-supplemented diet exerts numerous health benefits, but high levels of oxalic acid and nitrate can cause medical problems, so their levels should be reduced, while the levels of vitamins and phytochemicals could be further increased by breeding. Conventional spinach breeding is limited by the very complex sex determination. However, these limitations could be circumvented in synergy with a biotechnological approach. Accordingly, tissue culture techniques allow rapid and efficient clonal propagation of selected valuable genotypes, and somatic embryogenesis has been recognized as a superior process for clonal propagation because somatic embryos resemble zygotic embryos and therefore can spontaneously develop into complete plants. Since spinach has been considered recalcitrant to in vitro regeneration for decades, a deeper insight into the mechanisms underlying somatic embryogenesis is important for a better understanding and further improvement of the efficiency of this process. In this review, a comprehensive overview of the major factors affecting somatic embryogenesis in spinach is presented and discussed, with particular
emphasis on the synergistic effects of α–naphthaleneacetic acid, gibberellic acid, light, and the intrinsic predisposition of individual seedlings to somatic embryogenesis, as well as the expression of genes encoding key enzymes involved in the maintenance of gibberellin homeostasis and the levels of endogenous gibberellins.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Somatic Embryogenesis in Spinach—A Review
IS  - 9
VL  - 9
DO  - 10.3390/horticulturae9091048
SP  - 1048
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Belić, Maja and Ćalić, Dušica and Milojević, Jelena",
year = "2023",
abstract = "A spinach-supplemented diet exerts numerous health benefits, but high levels of oxalic acid and nitrate can cause medical problems, so their levels should be reduced, while the levels of vitamins and phytochemicals could be further increased by breeding. Conventional spinach breeding is limited by the very complex sex determination. However, these limitations could be circumvented in synergy with a biotechnological approach. Accordingly, tissue culture techniques allow rapid and efficient clonal propagation of selected valuable genotypes, and somatic embryogenesis has been recognized as a superior process for clonal propagation because somatic embryos resemble zygotic embryos and therefore can spontaneously develop into complete plants. Since spinach has been considered recalcitrant to in vitro regeneration for decades, a deeper insight into the mechanisms underlying somatic embryogenesis is important for a better understanding and further improvement of the efficiency of this process. In this review, a comprehensive overview of the major factors affecting somatic embryogenesis in spinach is presented and discussed, with particular
emphasis on the synergistic effects of α–naphthaleneacetic acid, gibberellic acid, light, and the intrinsic predisposition of individual seedlings to somatic embryogenesis, as well as the expression of genes encoding key enzymes involved in the maintenance of gibberellin homeostasis and the levels of endogenous gibberellins.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Somatic Embryogenesis in Spinach—A Review",
number = "9",
volume = "9",
doi = "10.3390/horticulturae9091048",
pages = "1048"
}

Zdravković-Korać, S., Belić, M., Ćalić, D.,& Milojević, J.. (2023). Somatic Embryogenesis in Spinach—A Review. in Horticulturae
Basel: MDPI., 9(9), 1048.
https://doi.org/10.3390/horticulturae9091048

Zdravković-Korać S, Belić M, Ćalić D, Milojević J. Somatic Embryogenesis in Spinach—A Review. in Horticulturae. 2023;9(9):1048.
doi:10.3390/horticulturae9091048 .

Zdravković-Korać, Snežana, Belić, Maja, Ćalić, Dušica, Milojević, Jelena, "Somatic Embryogenesis in Spinach—A Review" in Horticulturae, 9, no. 9 (2023):1048,
https://doi.org/10.3390/horticulturae9091048 . .

TY  - CONF
AU  - Belić, Maja
AU  - Tarkowská, Danuše
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5367
AB  - Gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) are essential for efficient somatic embryo (SE) induction from root apices of spinach seedlings, while neither is sufficient to provoke an embryogenic response. To gain insight into the role of gibberellins (GAs) in SE induction, the levels of bioactive GAs, their precursors and inactivation products, were determined in embryogenic and non-embryogenic explants, cultivated on medium supplemented with 20 μM NAA + 5 μM GA3 and 20 μM NAA, respectively, for 24 h and 7 days, and in control explants - root apices that were frozen immediately after isolation. The content of GAs was determined using ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The levels of biosynthetic GA precursors GA44, GA19 and GA20 were 7–18-fold higher in embryogenic explants, and only 1.1–2.8-fold higher in non-embryogenic explants compared to the control. In embryogenic explants, the levels of bioactive GA1, GA3, GA4 and GA7 increased 843-, 50433-, 60- and 136-fold, respectively, after 24 h, and remained high after 7 days of treatment. In non-embryogenic explants, only a transient increase of GA3 level (22-fold) was observed after 24 h of treatment. Expectedly, the products of GA2ox inactivation (especially GA8) were significantly higher in embryogenic than in non-embryogenic and control explants. The results evidence a positive impact of enhanced GA metabolism on the acquisition of embryogenic competence, confirming their important role in SE induction and indicating that the 13 hydroxylation pathway is a prevalent pathway of bioactive GA synthesis in embryogenic explants.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5367
ER  - 

@conference{
author = "Belić, Maja and Tarkowská, Danuše and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2022",
abstract = "Gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) are essential for efficient somatic embryo (SE) induction from root apices of spinach seedlings, while neither is sufficient to provoke an embryogenic response. To gain insight into the role of gibberellins (GAs) in SE induction, the levels of bioactive GAs, their precursors and inactivation products, were determined in embryogenic and non-embryogenic explants, cultivated on medium supplemented with 20 μM NAA + 5 μM GA3 and 20 μM NAA, respectively, for 24 h and 7 days, and in control explants - root apices that were frozen immediately after isolation. The content of GAs was determined using ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The levels of biosynthetic GA precursors GA44, GA19 and GA20 were 7–18-fold higher in embryogenic explants, and only 1.1–2.8-fold higher in non-embryogenic explants compared to the control. In embryogenic explants, the levels of bioactive GA1, GA3, GA4 and GA7 increased 843-, 50433-, 60- and 136-fold, respectively, after 24 h, and remained high after 7 days of treatment. In non-embryogenic explants, only a transient increase of GA3 level (22-fold) was observed after 24 h of treatment. Expectedly, the products of GA2ox inactivation (especially GA8) were significantly higher in embryogenic than in non-embryogenic and control explants. The results evidence a positive impact of enhanced GA metabolism on the acquisition of embryogenic competence, confirming their important role in SE induction and indicating that the 13 hydroxylation pathway is a prevalent pathway of bioactive GA synthesis in embryogenic explants.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5367"
}

Belić, M., Tarkowská, D., Zdravković-Korać, S.,& Milojević, J.. (2022). Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 26.
https://hdl.handle.net/21.15107/rcub_ibiss_5367

Belić M, Tarkowská D, Zdravković-Korać S, Milojević J. Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:26.
https://hdl.handle.net/21.15107/rcub_ibiss_5367 .

Belić, Maja, Tarkowská, Danuše, Zdravković-Korać, Snežana, Milojević, Jelena, "Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):26,
https://hdl.handle.net/21.15107/rcub_ibiss_5367 .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Ćalić, Dušica
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/277
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4777
AB  - Species of the genus Aesculus are very attractive woody ornamentals. Their organs contain numerous health-promoting phytochemicals. The most valuable of them—aescin—is used in commercial preparations for the treatment of venous insufficiency. The industrial source of aescin is horse chestnut seeds because the zygotic embryos are the main site of its accumulation. Horse chestnut somatic and zygotic embryos contain similar amount of aescin, hence somatic embryos could be exploited as an alternative source of aescin. Somatic embryogenesis, androgenesis and de novo shoot organogenesis were successfully achieved in several Aesculus species, as well as secondary somatic embryogenesis and shoot organogenesis, which enables mass production of embryos and shoots. In addition, an efficient method for cryopreservation of embryogenic tissue was established, assuring constant availability of the plant material. The developed methods are suitable for clonal propagation of elite specimens selected as the best aescin producers, the most attractive ornamentals or plants resistant to pests and diseases. These methods are also useful for molecular breeding purposes. Thus, in this review, the medicinal uses and a comprehensive survey of in vitro propagation methods established for Aesculus species, as well as the feasibility of in vitro production of aescin, are presented and discussed.
PB  - Basel: MDPI
T2  - Plants
T1  - Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review
IS  - 3
VL  - 11
DO  - 10.3390/plants11030277
SP  - 277
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Milojević, Jelena and Belić, Maja and Ćalić, Dušica",
year = "2022",
abstract = "Species of the genus Aesculus are very attractive woody ornamentals. Their organs contain numerous health-promoting phytochemicals. The most valuable of them—aescin—is used in commercial preparations for the treatment of venous insufficiency. The industrial source of aescin is horse chestnut seeds because the zygotic embryos are the main site of its accumulation. Horse chestnut somatic and zygotic embryos contain similar amount of aescin, hence somatic embryos could be exploited as an alternative source of aescin. Somatic embryogenesis, androgenesis and de novo shoot organogenesis were successfully achieved in several Aesculus species, as well as secondary somatic embryogenesis and shoot organogenesis, which enables mass production of embryos and shoots. In addition, an efficient method for cryopreservation of embryogenic tissue was established, assuring constant availability of the plant material. The developed methods are suitable for clonal propagation of elite specimens selected as the best aescin producers, the most attractive ornamentals or plants resistant to pests and diseases. These methods are also useful for molecular breeding purposes. Thus, in this review, the medicinal uses and a comprehensive survey of in vitro propagation methods established for Aesculus species, as well as the feasibility of in vitro production of aescin, are presented and discussed.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review",
number = "3",
volume = "11",
doi = "10.3390/plants11030277",
pages = "277"
}

Zdravković-Korać, S., Milojević, J., Belić, M.,& Ćalić, D.. (2022). Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review. in Plants
Basel: MDPI., 11(3), 277.
https://doi.org/10.3390/plants11030277

Zdravković-Korać S, Milojević J, Belić M, Ćalić D. Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review. in Plants. 2022;11(3):277.
doi:10.3390/plants11030277 .

Zdravković-Korać, Snežana, Milojević, Jelena, Belić, Maja, Ćalić, Dušica, "Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review" in Plants, 11, no. 3 (2022):277,
https://doi.org/10.3390/plants11030277 . .

TY  - CONF
AU  - Milojević, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5368
AB  - Spinach is a dioecious plant species with complex sex determination, which limits the success of its classical breeding. Hence, a biotechnological approach has been recognized as a promising alternative. Elucidation of the mechanism underlying somatic embryo (SE) induction and improvement of its efficiency have been the main goals of many years of research in this plant species recalcitrant to in vitro regeneration. Results have evidenced that the interplay among auxin, gibberellins (GA) and light was crucial for the induction of somatic embryogenesis from the root apices. None of these factors can be substituted for each other and only the lines with inherited high embryogenic capacity could respond in the absence of any of them, but with a very limited efficiency. The highest embryogenic response was obtained from the explants cultivated on medium supplemented with 20 μM α naphthaleneacetic acid (NAA) + 5 μM gibberellic acid (GA3) under blue light. Contrary to expectations, paclobutrazol, an inhibitor of bioactive gibberellin biosynthesis, acted synergistically with NAA and GA3 and further promoted somatic embryogenesis. In embryogenic explants, the expression levels of genes encoding the key enzymes involved in GA biosynthesis decreased, while those of genes involved in GA inactivation increased compared to non-embryogenic ones. Analysis of GA content using ultra-high performance liquid chromatography revealed enhanced GA metabolism in embryogenic explants during the first week of SE induction. During this period, the levels of endogenous bioactive GAs (GA1, GA3, GA4 and GA7) dramatically increased, confirming their important role in the acquisition of embryogenic potential.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Elucidation of the mechanism underlying somatic embryo induction in spinach
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5368
ER  - 

@conference{
author = "Milojević, Jelena and Zdravković-Korać, Snežana and Belić, Maja",
year = "2022",
abstract = "Spinach is a dioecious plant species with complex sex determination, which limits the success of its classical breeding. Hence, a biotechnological approach has been recognized as a promising alternative. Elucidation of the mechanism underlying somatic embryo (SE) induction and improvement of its efficiency have been the main goals of many years of research in this plant species recalcitrant to in vitro regeneration. Results have evidenced that the interplay among auxin, gibberellins (GA) and light was crucial for the induction of somatic embryogenesis from the root apices. None of these factors can be substituted for each other and only the lines with inherited high embryogenic capacity could respond in the absence of any of them, but with a very limited efficiency. The highest embryogenic response was obtained from the explants cultivated on medium supplemented with 20 μM α naphthaleneacetic acid (NAA) + 5 μM gibberellic acid (GA3) under blue light. Contrary to expectations, paclobutrazol, an inhibitor of bioactive gibberellin biosynthesis, acted synergistically with NAA and GA3 and further promoted somatic embryogenesis. In embryogenic explants, the expression levels of genes encoding the key enzymes involved in GA biosynthesis decreased, while those of genes involved in GA inactivation increased compared to non-embryogenic ones. Analysis of GA content using ultra-high performance liquid chromatography revealed enhanced GA metabolism in embryogenic explants during the first week of SE induction. During this period, the levels of endogenous bioactive GAs (GA1, GA3, GA4 and GA7) dramatically increased, confirming their important role in the acquisition of embryogenic potential.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Elucidation of the mechanism underlying somatic embryo induction in spinach",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5368"
}

Milojević, J., Zdravković-Korać, S.,& Belić, M.. (2022). Elucidation of the mechanism underlying somatic embryo induction in spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 134.
https://hdl.handle.net/21.15107/rcub_ibiss_5368

Milojević J, Zdravković-Korać S, Belić M. Elucidation of the mechanism underlying somatic embryo induction in spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:134.
https://hdl.handle.net/21.15107/rcub_ibiss_5368 .

Milojević, Jelena, Zdravković-Korać, Snežana, Belić, Maja, "Elucidation of the mechanism underlying somatic embryo induction in spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):134,
https://hdl.handle.net/21.15107/rcub_ibiss_5368 .

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5366
AB  - Gibberellic acid (GA3) is indispensable for somatic embryo (SE) induction from root apices of spinach. To elucidate its role in SE induction, paclobutrazol (PAC), an inhibitor of gibberellin biosynthesis, was used. Root apices (1 cm) isolated from SE-derived, in vitro-cultivated plants of a previously selected line with high embryogenic capacity were used for experimentation. The explants were cultivated on media containing 20 μM α-naphthaleneacetic acid (NAA) + 0 or 5 μM GA3 + 0, 1, 2.5, 5 or 10 μM PAC. NAA alone induced SE regeneration in 89% of the explants, but with only 3.5 SEs per explant, while the explants cultivated on media supplemented with NAA+GA3 or NAA +
GA3 + 2.5 μM PAC regenerated at 100% with 17.7 and 34.6 SEs per explant, respectively. However, in the absence of GA3, higher levels of PAC were needed: 5-10 μM PAC + NAA for 100%-response and 15-22.2 SEs per explant. To get insight into the NAA-GA3-PAC interaction, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and degradation (GA2-oxidase) was analyzed in the explants during 28 days of SE-induction period, using quantitative real-time PCR. In the explants with high embryogenic capacity, the expression levels of SoGA20 ox and SoGA3 ox were significantly lower, and SoGA2-ox1 and SoGA2-ox3 significantly higher than in those with low embryogenic capacity, cultivated on medium with NAA alone, during the whole SE induction period, indicating that NAA-GA3-PAC interaction enabled the acquisition of embryogenic capacity by impacting GA metabolism.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5366
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2022",
abstract = "Gibberellic acid (GA3) is indispensable for somatic embryo (SE) induction from root apices of spinach. To elucidate its role in SE induction, paclobutrazol (PAC), an inhibitor of gibberellin biosynthesis, was used. Root apices (1 cm) isolated from SE-derived, in vitro-cultivated plants of a previously selected line with high embryogenic capacity were used for experimentation. The explants were cultivated on media containing 20 μM α-naphthaleneacetic acid (NAA) + 0 or 5 μM GA3 + 0, 1, 2.5, 5 or 10 μM PAC. NAA alone induced SE regeneration in 89% of the explants, but with only 3.5 SEs per explant, while the explants cultivated on media supplemented with NAA+GA3 or NAA +
GA3 + 2.5 μM PAC regenerated at 100% with 17.7 and 34.6 SEs per explant, respectively. However, in the absence of GA3, higher levels of PAC were needed: 5-10 μM PAC + NAA for 100%-response and 15-22.2 SEs per explant. To get insight into the NAA-GA3-PAC interaction, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and degradation (GA2-oxidase) was analyzed in the explants during 28 days of SE-induction period, using quantitative real-time PCR. In the explants with high embryogenic capacity, the expression levels of SoGA20 ox and SoGA3 ox were significantly lower, and SoGA2-ox1 and SoGA2-ox3 significantly higher than in those with low embryogenic capacity, cultivated on medium with NAA alone, during the whole SE induction period, indicating that NAA-GA3-PAC interaction enabled the acquisition of embryogenic capacity by impacting GA metabolism.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5366"
}

Belić, M., Zdravković-Korać, S.,& Milojević, J.. (2022). Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 27.
https://hdl.handle.net/21.15107/rcub_ibiss_5366

Belić M, Zdravković-Korać S, Milojević J. Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:27.
https://hdl.handle.net/21.15107/rcub_ibiss_5366 .

Belić, Maja, Zdravković-Korać, Snežana, Milojević, Jelena, "Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):27,
https://hdl.handle.net/21.15107/rcub_ibiss_5366 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5331
AB  - Epigenetic modifications of chromatin play a pivotal role in regulation of expression of genes involved in somatic embryo (SE) induction. Hence, the compounds which affect DNA-histone interaction may trigger somatic embryogenesis. Trichostatin (TSA) is a potent inhibitor of histone deacetylases, whose activity leads to increased histone acetylation, thereby affecting gene expression. To explore epigenetic control of SE regeneration from root apices (1 cm) of spinach seedlings, the explants were cultivated on media supplemented with 0, 0.1, 0.5, 1 or 5 μM TSA + 0, 1, 10 or 20 μM α naphthaleneacetic acid (NAA) + 0 or 5 μM gibberellic acid (GA3). The explants were exposed to TSA for 1, 7 or 14 days, and subsequently subcultivated on TSA free medium of the same composition. TSA was dissolved in dimethyl sulfoxide (DMSO), whose final concentration in all media, including TSA-free controls, was 0.05%. TSA was not sufficient to induce SEs either alone or in combination with NAA or GA3. SE regeneration was observed only from the explants cultivated on media supplemented with 10 or 20 μM NAA + 5 μM GA3. In both combinations, TSA promoted somatic embryogenesis, but longer TSA treatment was needed with 10 μM NAA than with 20 μMNAA for efficient SE induction. The highest embryogenic response was attained with 0.1-0.5 μM TSA. DMSO also significantly improved SE induction, probably by enhancing NAA and GA3 intake into the plant cells. The results indicate a significant role of epigenetic control in SE induction in spinach.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5331
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Belić, Maja and Ćalić, Dušica and Milojević, Jelena",
year = "2022",
abstract = "Epigenetic modifications of chromatin play a pivotal role in regulation of expression of genes involved in somatic embryo (SE) induction. Hence, the compounds which affect DNA-histone interaction may trigger somatic embryogenesis. Trichostatin (TSA) is a potent inhibitor of histone deacetylases, whose activity leads to increased histone acetylation, thereby affecting gene expression. To explore epigenetic control of SE regeneration from root apices (1 cm) of spinach seedlings, the explants were cultivated on media supplemented with 0, 0.1, 0.5, 1 or 5 μM TSA + 0, 1, 10 or 20 μM α naphthaleneacetic acid (NAA) + 0 or 5 μM gibberellic acid (GA3). The explants were exposed to TSA for 1, 7 or 14 days, and subsequently subcultivated on TSA free medium of the same composition. TSA was dissolved in dimethyl sulfoxide (DMSO), whose final concentration in all media, including TSA-free controls, was 0.05%. TSA was not sufficient to induce SEs either alone or in combination with NAA or GA3. SE regeneration was observed only from the explants cultivated on media supplemented with 10 or 20 μM NAA + 5 μM GA3. In both combinations, TSA promoted somatic embryogenesis, but longer TSA treatment was needed with 10 μM NAA than with 20 μMNAA for efficient SE induction. The highest embryogenic response was attained with 0.1-0.5 μM TSA. DMSO also significantly improved SE induction, probably by enhancing NAA and GA3 intake into the plant cells. The results indicate a significant role of epigenetic control in SE induction in spinach.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5331"
}

Zdravković-Korać, S., Belić, M., Ćalić, D.,& Milojević, J.. (2022). Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 25.
https://hdl.handle.net/21.15107/rcub_ibiss_5331

Zdravković-Korać S, Belić M, Ćalić D, Milojević J. Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:25.
https://hdl.handle.net/21.15107/rcub_ibiss_5331 .

Zdravković-Korać, Snežana, Belić, Maja, Ćalić, Dušica, Milojević, Jelena, "Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):25,
https://hdl.handle.net/21.15107/rcub_ibiss_5331 .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Ristić Djurović, Jasna
AU  - Ćirković, Saša
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5152
AB  - Стимулација семена магнетним пољем је биолошки безбедна, јефтина, популарна, еколошки прихватљива и неинвазивна метода са благотворним ефектима на биљке. Примена магнетних поља смањује употребу пестицида, инсектицида и омогућава производњу еколошки безбедне хране (Maffei1), што је у скаду са стратегијом ЕУ “Од њиве до вилице” (“Farm to fork”). Стратегија предвиђа да се до 2030. године смањи употреба пестицида за 50% и обезбеди удео еколошке пољопривредне производње од 25% у укупној пољопривреди. С обзиром на тренутних 0.60% површина под органском производњом у Србији, значајније повећање постаје реално само уз примену иновативних начина гајења пољопривредних култура. У овој студији, магнетна поља различитих јачина (50, 100 и 300 мТ) и времена експозиције показала су позитиван ефекат на клијавост семена купуса, раст клијанаца, дужину и ширину корена, број формираних листова, масу главице, садржај хлорофила, каротеноида и ксантофила, садржај каталаза и пероксидаза, витамина Ц, микро и макро елемената. У листовима третираног купуса детектовано је до 50 % више гвожђа, и два пута мање натријума него у контроли. Принос третираног купуса на огледном пољу земљорадничке задруге „Пољо-флора“ нашег највећег произвођача и извозника киселог купуса је 30 % повећан у односу на контролу. Добијени резултати сугеришу да се магнетна поља могу користити у органској пољопривреди и производњи здраве хране, што је у складу са стратегијом ЕУ.
AB  - Stimulacija semena magnetnim poljem je biološki bezbedna, jeftina, popularna, ekološki prihvatljiva i neinvazivna metoda sa blagotvornim efektima na biljke. Primena magnetnih polja smanjuje upotrebu pesticida, insekticida i omogućava proizvodnju ekološki bezbedne hrane (Maffei1), što je u skadu sa strategijom EU “Od njive do vilice” (“Farm to fork”). Strategija predviđa da se do 2030. godine smanji upotreba pesticida za 50% i obezbedi udeo ekološke poljoprivredne proizvodnje od 25% u ukupnoj poljoprivredi. S obzirom na trenutnih 0.60% površina pod organskom proizvodnjom u Srbiji, značajnije povećanje postaje realno samo uz primenu inovativnih načina gajenja poljoprivrednih kultura. U ovoj studiji, magnetna polja različitih jačina (50, 100 i 300 mT) i vremena ekspozicije pokazala su pozitivan efekat na klijavost semena kupusa, rast klijanaca, dužinu i širinu korena, broj formiranih listova, masu glavice, sadržaj hlorofila, karotenoida i ksantofila, sadržaj katalaza i peroksidaza, vitamina C, mikro i makro elemenata. U listovima tretiranog kupusa detektovano je do 50 % više gvožđa, i dva puta manje natrijuma nego u kontroli. Prinos tretiranog kupusa na oglednom polju zemljoradničke zadruge „Poljo-flora“ našeg najvećeg proizvođača i izvoznika kiselog kupusa je 30 % povećan u odnosu na kontrolu. Dobijeni rezultati sugerišu da se magnetna polja mogu koristiti u organskoj poljoprivredi i proizvodnji zdrave hrane, što je u skladu sa strategijom EU.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Могу ли магнети помоћи биљкама?
T1  - Mogu li magneti pomoći biljkama?
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5152
ER  - 

@conference{
author = "Ćalić, Dušica and Ristić Djurović, Jasna and Ćirković, Saša and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana",
year = "2022",
abstract = "Стимулација семена магнетним пољем је биолошки безбедна, јефтина, популарна, еколошки прихватљива и неинвазивна метода са благотворним ефектима на биљке. Примена магнетних поља смањује употребу пестицида, инсектицида и омогућава производњу еколошки безбедне хране (Maffei1), што је у скаду са стратегијом ЕУ “Од њиве до вилице” (“Farm to fork”). Стратегија предвиђа да се до 2030. године смањи употреба пестицида за 50% и обезбеди удео еколошке пољопривредне производње од 25% у укупној пољопривреди. С обзиром на тренутних 0.60% површина под органском производњом у Србији, значајније повећање постаје реално само уз примену иновативних начина гајења пољопривредних култура. У овој студији, магнетна поља различитих јачина (50, 100 и 300 мТ) и времена експозиције показала су позитиван ефекат на клијавост семена купуса, раст клијанаца, дужину и ширину корена, број формираних листова, масу главице, садржај хлорофила, каротеноида и ксантофила, садржај каталаза и пероксидаза, витамина Ц, микро и макро елемената. У листовима третираног купуса детектовано је до 50 % више гвожђа, и два пута мање натријума него у контроли. Принос третираног купуса на огледном пољу земљорадничке задруге „Пољо-флора“ нашег највећег произвођача и извозника киселог купуса је 30 % повећан у односу на контролу. Добијени резултати сугеришу да се магнетна поља могу користити у органској пољопривреди и производњи здраве хране, што је у складу са стратегијом ЕУ., Stimulacija semena magnetnim poljem je biološki bezbedna, jeftina, popularna, ekološki prihvatljiva i neinvazivna metoda sa blagotvornim efektima na biljke. Primena magnetnih polja smanjuje upotrebu pesticida, insekticida i omogućava proizvodnju ekološki bezbedne hrane (Maffei1), što je u skadu sa strategijom EU “Od njive do vilice” (“Farm to fork”). Strategija predviđa da se do 2030. godine smanji upotreba pesticida za 50% i obezbedi udeo ekološke poljoprivredne proizvodnje od 25% u ukupnoj poljoprivredi. S obzirom na trenutnih 0.60% površina pod organskom proizvodnjom u Srbiji, značajnije povećanje postaje realno samo uz primenu inovativnih načina gajenja poljoprivrednih kultura. U ovoj studiji, magnetna polja različitih jačina (50, 100 i 300 mT) i vremena ekspozicije pokazala su pozitivan efekat na klijavost semena kupusa, rast klijanaca, dužinu i širinu korena, broj formiranih listova, masu glavice, sadržaj hlorofila, karotenoida i ksantofila, sadržaj katalaza i peroksidaza, vitamina C, mikro i makro elemenata. U listovima tretiranog kupusa detektovano je do 50 % više gvožđa, i dva puta manje natrijuma nego u kontroli. Prinos tretiranog kupusa na oglednom polju zemljoradničke zadruge „Poljo-flora“ našeg najvećeg proizvođača i izvoznika kiselog kupusa je 30 % povećan u odnosu na kontrolu. Dobijeni rezultati sugerišu da se magnetna polja mogu koristiti u organskoj poljoprivredi i proizvodnji zdrave hrane, što je u skladu sa strategijom EU.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Могу ли магнети помоћи биљкама?, Mogu li magneti pomoći biljkama?",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5152"
}

Ćalić, D., Ristić Djurović, J., Ćirković, S., Milojević, J., Belić, M.,& Zdravković-Korać, S.. (2022). Могу ли магнети помоћи биљкама?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5152

Ćalić D, Ristić Djurović J, Ćirković S, Milojević J, Belić M, Zdravković-Korać S. Могу ли магнети помоћи биљкама?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5152 .

Ćalić, Dušica, Ristić Djurović, Jasna, Ćirković, Saša, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, "Могу ли магнети помоћи биљкама?" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5152 .

TY  - CONF
AU  - Jevremović, Slađana
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5153
AB  - Идеја о вештачким семенима датира још од 1977. године када је Murashige1 предложио да вештачка семена представљају „инкапсулиране соматске ембрионе“. Данас се под термином вештачка (синтетичка, артифицијална) семена предразумева било који инкапсулирани биљни материјал (соматски ембриони, врхови апикалних или аксиларних изданака, ћелијски агрегати) који се може користити за гајење, чување, пропагацију и добијање комплетних биљака. Основа технологије вештачких семена је имитирање услова за нормално растење и развиће биљака као што се дешава током клијања и формирања биљака из семена. Технологија вештачких семена је искоришћена и као део појединих протокола за краткотрајно чување биљног материјала методама успореног растења као и за његово дуготрајно чување методама криопрезервације. Ова технологија је од великог значаја за биљне врсте које производе невијабилна семена, семена која тешко клијају, формирају мали број семена или их уопште не производе. Неке од таквих биљних врста су и заштићене и угрожене врсте које најчешће показују проблеме у развићу семена и репродукцији. Такође, ова технологија се показала као погодна за пропагацију и чување различитих дрвенастих, лековитих или украсних биљака које се вегетативно умножавају. У раду ће бити приказана методологија добијања вештачких семена као и досадашњи резултати на примени технологије пропагације врхова изданака хризантема и ћелијских суспензија кестена.
AB  - Ideja o veštačkim semenima datira još od 1977. godine kada je Murashige1 predložio da veštačka semena predstavljaju „inkapsulirane somatske embrione“. Danas se pod terminom veštačka (sintetička, artificijalna) semena predrazumeva bilo koji inkapsulirani biljni materijal (somatski embrioni, vrhovi apikalnih ili aksilarnih izdanaka, ćelijski agregati) koji se može koristiti za gajenje, čuvanje, propagaciju i dobijanje kompletnih biljaka. Osnova tehnologije veštačkih semena je imitiranje uslova za normalno rastenje i razviće biljaka kao što se dešava tokom klijanja i formiranja biljaka iz semena. Tehnologija veštačkih semena je iskorišćena i kao deo pojedinih protokola za kratkotrajno čuvanje biljnog materijala metodama usporenog rastenja kao i za njegovo dugotrajno čuvanje metodama krioprezervacije. Ova tehnologija je od velikog značaja za biljne vrste koje proizvode nevijabilna semena, semena koja teško klijaju, formiraju mali broj semena ili ih uopšte ne proizvode. Neke od takvih biljnih vrsta su i zaštićene i ugrožene vrste koje najčešće pokazuju probleme u razviću semena i reprodukciji. Takođe, ova tehnologija se pokazala kao pogodna za propagaciju i čuvanje različitih drvenastih, lekovitih ili ukrasnih biljaka koje se vegetativno umnožavaju. U radu će biti prikazana metodologija dobijanja veštačkih semena kao i dosadašnji rezultati na primeni tehnologije propagacije vrhova izdanaka hrizantema i ćelijskih suspenzija kestena.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Примена технологије вештачких семена у пропагацији и  конзервацији биљака
T1  - Primena tehnologije veštačkih semena u propagaciji i konzervaciji biljaka
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5153
ER  - 

@conference{
author = "Jevremović, Slađana and Ćalić, Dušica and Zdravković-Korać, Snežana",
year = "2022",
abstract = "Идеја о вештачким семенима датира још од 1977. године када је Murashige1 предложио да вештачка семена представљају „инкапсулиране соматске ембрионе“. Данас се под термином вештачка (синтетичка, артифицијална) семена предразумева било који инкапсулирани биљни материјал (соматски ембриони, врхови апикалних или аксиларних изданака, ћелијски агрегати) који се може користити за гајење, чување, пропагацију и добијање комплетних биљака. Основа технологије вештачких семена је имитирање услова за нормално растење и развиће биљака као што се дешава током клијања и формирања биљака из семена. Технологија вештачких семена је искоришћена и као део појединих протокола за краткотрајно чување биљног материјала методама успореног растења као и за његово дуготрајно чување методама криопрезервације. Ова технологија је од великог значаја за биљне врсте које производе невијабилна семена, семена која тешко клијају, формирају мали број семена или их уопште не производе. Неке од таквих биљних врста су и заштићене и угрожене врсте које најчешће показују проблеме у развићу семена и репродукцији. Такође, ова технологија се показала као погодна за пропагацију и чување различитих дрвенастих, лековитих или украсних биљака које се вегетативно умножавају. У раду ће бити приказана методологија добијања вештачких семена као и досадашњи резултати на примени технологије пропагације врхова изданака хризантема и ћелијских суспензија кестена., Ideja o veštačkim semenima datira još od 1977. godine kada je Murashige1 predložio da veštačka semena predstavljaju „inkapsulirane somatske embrione“. Danas se pod terminom veštačka (sintetička, artificijalna) semena predrazumeva bilo koji inkapsulirani biljni materijal (somatski embrioni, vrhovi apikalnih ili aksilarnih izdanaka, ćelijski agregati) koji se može koristiti za gajenje, čuvanje, propagaciju i dobijanje kompletnih biljaka. Osnova tehnologije veštačkih semena je imitiranje uslova za normalno rastenje i razviće biljaka kao što se dešava tokom klijanja i formiranja biljaka iz semena. Tehnologija veštačkih semena je iskorišćena i kao deo pojedinih protokola za kratkotrajno čuvanje biljnog materijala metodama usporenog rastenja kao i za njegovo dugotrajno čuvanje metodama krioprezervacije. Ova tehnologija je od velikog značaja za biljne vrste koje proizvode nevijabilna semena, semena koja teško klijaju, formiraju mali broj semena ili ih uopšte ne proizvode. Neke od takvih biljnih vrsta su i zaštićene i ugrožene vrste koje najčešće pokazuju probleme u razviću semena i reprodukciji. Takođe, ova tehnologija se pokazala kao pogodna za propagaciju i čuvanje različitih drvenastih, lekovitih ili ukrasnih biljaka koje se vegetativno umnožavaju. U radu će biti prikazana metodologija dobijanja veštačkih semena kao i dosadašnji rezultati na primeni tehnologije propagacije vrhova izdanaka hrizantema i ćelijskih suspenzija kestena.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Примена технологије вештачких семена у пропагацији и  конзервацији биљака, Primena tehnologije veštačkih semena u propagaciji i konzervaciji biljaka",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5153"
}

Jevremović, S., Ćalić, D.,& Zdravković-Korać, S.. (2022). Примена технологије вештачких семена у пропагацији и  конзервацији биљака. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5153

Jevremović S, Ćalić D, Zdravković-Korać S. Примена технологије вештачких семена у пропагацији и  конзервацији биљака. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5153 .

Jevremović, Slađana, Ćalić, Dušica, Zdravković-Korać, Snežana, "Примена технологије вештачких семена у пропагацији и  конзервацији биљака" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5153 .

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5412
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
PB  - eas
C3  - Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5412
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture, eas",
journal = "Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5412"
}

Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
East Sarajevo : Faculty of Agriculture., 213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412

Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina. 2021;:213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina (2021):213,
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Miletić, Rade
AU  - Zdravković-Korać, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4319
AB  - Globalization has drastically reduced the number of autochthon apple cultivars in the Serbian market and most of them have nearly disappeared; however, some of these cultivars, such as Petrovača, Budimka, Kolačara Pozna, and Kožara, have extraordinary quality, good pomological characteristics, and pest and disease resistance. The present study was conducted to develop a protocol for the storage of pollen for further use in the conservation and breeding of these cultivars. Viability and germination of the mature pollen were tested in vitro, at four storage temperatures (20, 4, −20, and −80°C), right after harvest or 1, 2, 3, 4, 5, and 6 months after storage. Differences in fresh pollen viability and germination between cultivars were statistically significant and ranged from 60 to 88% and 59 to 98%, respectively. Fresh pollen of cv. Budimka showed the highest viability and germination in comparison with other cultivars, especially cv. Kožara. Pollen viability and germination decreased over the storage period, and it was the lowest after 6 months of storage at room temperature in all tested cultivars. Storage at 4°C prolonged the pollen viability and germinability of 1–5 fold, depending on the cultivar and treatment duration; however, the pollen longevity of all cultivars was significantly extended when stored at −20 or −80°C. After 6 months, pollen of cv. Budimka stored at −20 and −80°C showed 14–15 fold higher germination rates in relation to pollen storage at room temperature for the same period. The results of the present study suggest that the pollen of these apple cultivars could be efficiently maintained at −20°C and could be further used for breeding purposes, e.g., for crossings between cultivars that flower at different times of the year.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars
VL  - 12
DO  - 10.3389/fpls.2021.709231
SP  - 709231
ER  - 

@article{
author = "Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Miletić, Rade and Zdravković-Korać, Snežana",
year = "2021",
abstract = "Globalization has drastically reduced the number of autochthon apple cultivars in the Serbian market and most of them have nearly disappeared; however, some of these cultivars, such as Petrovača, Budimka, Kolačara Pozna, and Kožara, have extraordinary quality, good pomological characteristics, and pest and disease resistance. The present study was conducted to develop a protocol for the storage of pollen for further use in the conservation and breeding of these cultivars. Viability and germination of the mature pollen were tested in vitro, at four storage temperatures (20, 4, −20, and −80°C), right after harvest or 1, 2, 3, 4, 5, and 6 months after storage. Differences in fresh pollen viability and germination between cultivars were statistically significant and ranged from 60 to 88% and 59 to 98%, respectively. Fresh pollen of cv. Budimka showed the highest viability and germination in comparison with other cultivars, especially cv. Kožara. Pollen viability and germination decreased over the storage period, and it was the lowest after 6 months of storage at room temperature in all tested cultivars. Storage at 4°C prolonged the pollen viability and germinability of 1–5 fold, depending on the cultivar and treatment duration; however, the pollen longevity of all cultivars was significantly extended when stored at −20 or −80°C. After 6 months, pollen of cv. Budimka stored at −20 and −80°C showed 14–15 fold higher germination rates in relation to pollen storage at room temperature for the same period. The results of the present study suggest that the pollen of these apple cultivars could be efficiently maintained at −20°C and could be further used for breeding purposes, e.g., for crossings between cultivars that flower at different times of the year.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars",
volume = "12",
doi = "10.3389/fpls.2021.709231",
pages = "709231"
}

Ćalić, D., Milojević, J., Belić, M., Miletić, R.,& Zdravković-Korać, S.. (2021). Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 12, 709231.
https://doi.org/10.3389/fpls.2021.709231

Ćalić D, Milojević J, Belić M, Miletić R, Zdravković-Korać S. Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars. in Frontiers in Plant Science. 2021;12:709231.
doi:10.3389/fpls.2021.709231 .

Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Miletić, Rade, Zdravković-Korać, Snežana, "Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars" in Frontiers in Plant Science, 12 (2021):709231,
https://doi.org/10.3389/fpls.2021.709231 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - CHAP
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3949
UR  - https://novapublishers.com/shop/allium-ecology-distribution-and-cultivation/
PB  - Hauppauge, New York : Nova Science Publishers
T2  - Allium: Ecology, Distribution and Cultivation
T1  - Biotechnological Approach for Cultivation of Allium Species
SP  - 187
EP  - 260
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3949
ER  - 

@inbook{
author = "Zdravković-Korać, Snežana and Belić, Maja and Milojević, Jelena",
year = "2020",
publisher = "Hauppauge, New York : Nova Science Publishers",
journal = "Allium: Ecology, Distribution and Cultivation",
booktitle = "Biotechnological Approach for Cultivation of Allium Species",
pages = "187-260",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3949"
}

Zdravković-Korać, S., Belić, M.,& Milojević, J.. (2020). Biotechnological Approach for Cultivation of Allium Species. in Allium: Ecology, Distribution and Cultivation
Hauppauge, New York : Nova Science Publishers., 187-260.
https://hdl.handle.net/21.15107/rcub_ibiss_3949

Zdravković-Korać S, Belić M, Milojević J. Biotechnological Approach for Cultivation of Allium Species. in Allium: Ecology, Distribution and Cultivation. 2020;:187-260.
https://hdl.handle.net/21.15107/rcub_ibiss_3949 .

Zdravković-Korać, Snežana, Belić, Maja, Milojević, Jelena, "Biotechnological Approach for Cultivation of Allium Species" in Allium: Ecology, Distribution and Cultivation (2020):187-260,
https://hdl.handle.net/21.15107/rcub_ibiss_3949 .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Ćalić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3997
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
SP  - 19290
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Ćalić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9",
pages = "19290"
}

Belić, M., Zdravković-Korać, S., Uzelac, B., Ćalić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research., 10(1), 19290.
https://doi.org/10.1038/s41598-020-76279-9

Belić M, Zdravković-Korać S, Uzelac B, Ćalić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1):19290.
doi:10.1038/s41598-020-76279-9 .

Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Ćalić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020):19290,
https://doi.org/10.1038/s41598-020-76279-9 . .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Janošević, Dušica
AU  - Savić, Jelena
AU  - Todorović, Slađana
AU  - Banjac, Nevena
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3774
AB  - Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach
VL  - 142
DO  - 10.1007/s11240-020-01878-3
SP  - 537
EP  - 548
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Janošević, Dušica and Savić, Jelena and Todorović, Slađana and Banjac, Nevena and Milojević, Jelena",
year = "2020",
abstract = "Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach",
volume = "142",
doi = "10.1007/s11240-020-01878-3",
pages = "537-548"
}

Belić, M., Zdravković-Korać, S., Janošević, D., Savić, J., Todorović, S., Banjac, N.,& Milojević, J.. (2020). Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture
Springer., 142, 537-548.
https://doi.org/10.1007/s11240-020-01878-3

Belić M, Zdravković-Korać S, Janošević D, Savić J, Todorović S, Banjac N, Milojević J. Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture. 2020;142:537-548.
doi:10.1007/s11240-020-01878-3 .

Belić, Maja, Zdravković-Korać, Snežana, Janošević, Dušica, Savić, Jelena, Todorović, Slađana, Banjac, Nevena, Milojević, Jelena, "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach" in Plant Cell, Tissue and Organ Culture, 142 (2020):537-548,
https://doi.org/10.1007/s11240-020-01878-3 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Matekalo, Dragana
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161718304140?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3213
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.
T2  - Journal of Plant Physiology
T1  - Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.
VL  - 232
DO  - 10.1016/j.jplph.2018.11.004
SP  - 257
EP  - 269
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Savić, Jelena and Cingel, Aleksandar and Matekalo, Dragana and Zdravković-Korać, Snežana and Ninković, Slavica",
year = "2019",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.",
journal = "Journal of Plant Physiology",
title = "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.",
volume = "232",
doi = "10.1016/j.jplph.2018.11.004",
pages = "257-269"
}

Ćosić, T., Raspor, M., Savić, J., Cingel, A., Matekalo, D., Zdravković-Korać, S.,& Ninković, S.. (2019). Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology, 232, 257-269.
https://doi.org/10.1016/j.jplph.2018.11.004

Ćosić T, Raspor M, Savić J, Cingel A, Matekalo D, Zdravković-Korać S, Ninković S. Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology. 2019;232:257-269.
doi:10.1016/j.jplph.2018.11.004 .

Ćosić, Tatjana, Raspor, Martin, Savić, Jelena, Cingel, Aleksandar, Matekalo, Dragana, Zdravković-Korać, Snežana, Ninković, Slavica, "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi." in Journal of Plant Physiology, 232 (2019):257-269,
https://doi.org/10.1016/j.jplph.2018.11.004 . .

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5592
AB  - Gibberellins (GA) play a pivotal role in the induction of somatic embryogenesis (SE) from the apical root sections of spinach seedlings. To study the role of GA in this process, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-ox and GA3-ox) and degradation (GA2-ox1, -ox2 and -ox3) was analysed in the explants cultivated on inductive medium (IM - supplemented with 20 μM NAA + 5 μM GA3), noninductive medium (NM - containing 20 μM NAA), or plant growth regulator (PGR)-free medium. Expression of these genes was monitored by means of quantitative real-time PCR during 28 days SE-induction period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a control. In the IM-cultivated explants, expression of both GA20-ox and GA3-ox decreased over the whole induction period. On the other hand, in the explants cultivated on NM or PGR-free medium expression of these genes began to increase after 1 or 14 days, respectively, and remained higher than in the control steadily over time. Concerning the catabolic GA2-ox genes, particularly GA2-ox2, their expression was higher in the IM-cultivated explants over the whole SE-induction period comparing to the control. However, in the NM-cultivated explants, expression of GA2-ox2 and GA2-ox3 was mostly lower. The results indicate that continuous expression level decrease in GA20-ox and GA3-ox and increase in GA2-ox2 favor SE regeneration from root-tips of spinach. Understanding the role of GA in SE induction in spinach would not only provide a deeper insight into the mechanism of this process, but also enhance the improvement of this species through biotechnological approach.
PB  - Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences
C3  - Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic
T1  - Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5592
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2019",
abstract = "Gibberellins (GA) play a pivotal role in the induction of somatic embryogenesis (SE) from the apical root sections of spinach seedlings. To study the role of GA in this process, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-ox and GA3-ox) and degradation (GA2-ox1, -ox2 and -ox3) was analysed in the explants cultivated on inductive medium (IM - supplemented with 20 μM NAA + 5 μM GA3), noninductive medium (NM - containing 20 μM NAA), or plant growth regulator (PGR)-free medium. Expression of these genes was monitored by means of quantitative real-time PCR during 28 days SE-induction period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a control. In the IM-cultivated explants, expression of both GA20-ox and GA3-ox decreased over the whole induction period. On the other hand, in the explants cultivated on NM or PGR-free medium expression of these genes began to increase after 1 or 14 days, respectively, and remained higher than in the control steadily over time. Concerning the catabolic GA2-ox genes, particularly GA2-ox2, their expression was higher in the IM-cultivated explants over the whole SE-induction period comparing to the control. However, in the NM-cultivated explants, expression of GA2-ox2 and GA2-ox3 was mostly lower. The results indicate that continuous expression level decrease in GA20-ox and GA3-ox and increase in GA2-ox2 favor SE regeneration from root-tips of spinach. Understanding the role of GA in SE induction in spinach would not only provide a deeper insight into the mechanism of this process, but also enhance the improvement of this species through biotechnological approach.",
publisher = "Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences",
journal = "Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic",
title = "Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5592"
}

Belić, M., Zdravković-Korać, S.,& Milojević, J.. (2019). Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction. in Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic
Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_5592

Belić M, Zdravković-Korać S, Milojević J. Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction. in Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic. 2019;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_5592 .

Belić, Maja, Zdravković-Korać, Snežana, Milojević, Jelena, "Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction" in Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic (2019):39,
https://hdl.handle.net/21.15107/rcub_ibiss_5592 .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Savić, Jelena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0304423818308938?via%3Dihub
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0304423818308938
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3224
AB  - Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.
T2  - Scientia Horticulturae
T1  - Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus
VL  - 247
DO  - 10.1016/j.scienta.2018.12.021
SP  - 362
EP  - 372
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Devrnja, Nina and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Savić, Jelena",
year = "2019",
abstract = "Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.",
journal = "Scientia Horticulturae",
title = "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus",
volume = "247",
doi = "10.1016/j.scienta.2018.12.021",
pages = "362-372"
}

Zdravković-Korać, S., Tubić, L., Devrnja, N., Ćalić, D., Milojević, J., Belić, M.,& Savić, J.. (2019). Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae, 247, 362-372.
https://doi.org/10.1016/j.scienta.2018.12.021

Zdravković-Korać S, Tubić L, Devrnja N, Ćalić D, Milojević J, Belić M, Savić J. Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae. 2019;247:362-372.
doi:10.1016/j.scienta.2018.12.021 .

Zdravković-Korać, Snežana, Tubić, Ljiljana, Devrnja, Nina, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Savić, Jelena, "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus" in Scientia Horticulturae, 247 (2019):362-372,
https://doi.org/10.1016/j.scienta.2018.12.021 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Ghalawenji, Nabil
AU  - Zdravković-Korać, Snežana
PY  - 2019
UR  - http://link.springer.com/10.1007/s11240-019-01595-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3310
AB  - Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)
DO  - 10.1007/s11240-019-01595-6
ER  - 

@article{
author = "Banjac, Nevena and Vinterhalter, Branka and Krstić Milošević, Dijana and Milojević, Jelena and Tubić, Ljiljana and Ghalawenji, Nabil and Zdravković-Korać, Snežana",
year = "2019",
abstract = "Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)",
doi = "10.1007/s11240-019-01595-6"
}

Banjac, N., Vinterhalter, B., Krstić Milošević, D., Milojević, J., Tubić, L., Ghalawenji, N.,& Zdravković-Korać, S.. (2019). Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC).
https://doi.org/10.1007/s11240-019-01595-6

Banjac N, Vinterhalter B, Krstić Milošević D, Milojević J, Tubić L, Ghalawenji N, Zdravković-Korać S. Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC). 2019;.
doi:10.1007/s11240-019-01595-6 .

Banjac, Nevena, Vinterhalter, Branka, Krstić Milošević, Dijana, Milojević, Jelena, Tubić, Ljiljana, Ghalawenji, Nabil, Zdravković-Korać, Snežana, "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)" in Plant Cell, Tissue and Organ Culture (PCTOC) (2019),
https://doi.org/10.1007/s11240-019-01595-6 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan
AU  - Krstić Milošević, Dijana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0926669018311282?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3230
AB  - Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.
T2  - Industrial Crops and Products
T1  - Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants
VL  - 130
DO  - 10.1016/J.INDCROP.2018.12.066
SP  - 216
EP  - 229
ER  - 

@article{
author = "Vinterhalter, Branka and Savić, Jelena and Zdravković-Korać, Snežana and Banjac, Nevena and Vinterhalter, Dragan and Krstić Milošević, Dijana",
year = "2019",
abstract = "Production of innovative drugs from natural products in controlled conditions plays an important role in modern pharmacology in order to tackle global health challenges. The potential of Gentiana utriculosa hairy roots, obtained by transformation with Agrobacterium rhizogenes A4M70GUS, as well as that of the shoots regenerated from them via somatic embryogenesis, for xanthone production was investigated. Gentiana utriculosa was shown to be a new source of xanthones, medicinal raw materials for different pharmaceutical applications, among which decussatin has been recently recognized as a prospective hepatoprotective and antiulcer compound. Decussatin and decussatin-1-O-primeveroside were detected in both hairy roots and transgenic shoots, while mangiferin, present in nontransformed plants, was not detected. Quantitative HPLC analysis revealed up to 4.5-fold higher decussatin production in hairy roots line 9 compared with the plants from the nature, and this clone was selected. It showed stable growth after more than two years of continuous subcultivation. Cultivation of hairy roots on solid medium was favorable for biomass production, while liquid culture was beneficial for decussatin and decussatin-1-O-primeveroside accumulation. Cytokinins kinetin or N6-benzyladenine promoted somatic embryo maturation and germination as well as multiplication of obtained plantlets, while active charcoal reduced hyperhydricity. Plants regenerated from selected hairy root line 9 comprised two TL-DNA inserts, as confirmed by Southern blot analysis. They accumulated at least 2.5-fold more decussatin then nontransformed plants. Therefore, these plants could be valuable material to create xanthone high-yielding cultivars of G. utriculosa.",
journal = "Industrial Crops and Products",
title = "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants",
volume = "130",
doi = "10.1016/J.INDCROP.2018.12.066",
pages = "216-229"
}

Vinterhalter, B., Savić, J., Zdravković-Korać, S., Banjac, N., Vinterhalter, D.,& Krstić Milošević, D.. (2019). Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products, 130, 216-229.
https://doi.org/10.1016/J.INDCROP.2018.12.066

Vinterhalter B, Savić J, Zdravković-Korać S, Banjac N, Vinterhalter D, Krstić Milošević D. Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants. in Industrial Crops and Products. 2019;130:216-229.
doi:10.1016/J.INDCROP.2018.12.066 .

Vinterhalter, Branka, Savić, Jelena, Zdravković-Korać, Snežana, Banjac, Nevena, Vinterhalter, Dragan, Krstić Milošević, Dijana, "Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants" in Industrial Crops and Products, 130 (2019):216-229,
https://doi.org/10.1016/J.INDCROP.2018.12.066 . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Nikolić, Radomirka
AU  - Banjac, Nevena
AU  - Zdravković-Korać, Snežana
AU  - Stupar, Sofija
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161719301786?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3490
AB  - Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.
T2  - Journal of Plant Physiology
T1  - Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.
VL  - 243
DO  - 10.1016/j.jplph.2019.153055
SP  - 153055
ER  - 

@article{
author = "Savić, Jelena and Nikolić, Radomirka and Banjac, Nevena and Zdravković-Korać, Snežana and Stupar, Sofija and Cingel, Aleksandar and Ćosić, Tatjana and Raspor, Martin and Smigocki, Ann and Ninković, Slavica",
year = "2019",
abstract = "Food demands of increasing human population dictate intensification of livestock production, however, environmental stresses could jeopardize producers' efforts. Forage legumes suffer from yield losses and poor nutritional status due to salinity increase of agricultural soils. As tools aimed to reduce negative impacts of biotic or abiotic stresses, proteinase inhibitors (PIs) have been promoted for biotechnological improvements. In order to increase tolerance of Lotus corniculatus L. to salt stress, serine PI, BvSTI, was introduced into this legume using Agrobacterium rhizogenes, with final transformation efficiency of 4.57%. PCR, DNA gel-blot, RT-PCR and in-gel protein activity assays confirmed the presence and activity of BvSTI products in transformed lines. Plants from three selected transgenic lines (21, 73 and 109) showed significant alterations in overall phenotypic appearance, corresponding to differences in BvSTI accumulation. Lines 73 and 109 showed up to 7.3-fold higher number of tillers and massive, up to 5.8-fold heavier roots than in nontransformed controls (NTC). Line 21 was phenotypically similar to NTC, accumulated less BvSTI transcripts and did not exhibit an additional band of recombinant trypsin inhibitor as seen in lines 73 and 109. Exposure of the transgenic lines to NaCl revealed different levels of salt stress susceptibility. The NaCl sensitivity index, based on morphological appearance and chlorophyll concentrations showed that lines 73 and 109 were significantly less affected by salinity than NTC or line 21. High level of BvSTI altered morphology and delayed salt stress related senescence, implicating BvSTI gene as a promising tool for salinity tolerance improvement trials in L. corniculatus.",
journal = "Journal of Plant Physiology",
title = "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.",
volume = "243",
doi = "10.1016/j.jplph.2019.153055",
pages = "153055"
}

Savić, J., Nikolić, R., Banjac, N., Zdravković-Korać, S., Stupar, S., Cingel, A., Ćosić, T., Raspor, M., Smigocki, A.,& Ninković, S.. (2019). Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology, 243, 153055.
https://doi.org/10.1016/j.jplph.2019.153055

Savić J, Nikolić R, Banjac N, Zdravković-Korać S, Stupar S, Cingel A, Ćosić T, Raspor M, Smigocki A, Ninković S. Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.. in Journal of Plant Physiology. 2019;243:153055.
doi:10.1016/j.jplph.2019.153055 .

Savić, Jelena, Nikolić, Radomirka, Banjac, Nevena, Zdravković-Korać, Snežana, Stupar, Sofija, Cingel, Aleksandar, Ćosić, Tatjana, Raspor, Martin, Smigocki, Ann, Ninković, Slavica, "Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L." in Journal of Plant Physiology, 243 (2019):153055,
https://doi.org/10.1016/j.jplph.2019.153055 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Ćuković, Katarina
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900026D
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4099
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3568
AB  - Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment.
T2  - Archives of Biological Sciences
T1  - Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach
IS  - 3
VL  - 71
DO  - 10.2298/ABS190315026D
SP  - 443
EP  - 453
ER  - 

@article{
author = "Dragićević, Milan and Ćuković, Katarina and Zdravković-Korać, Snežana and Simonović, Ana and Bogdanović, Milica and Todorović, Slađana",
year = "2019",
abstract = "Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment.",
journal = "Archives of Biological Sciences",
title = "Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach",
number = "3",
volume = "71",
doi = "10.2298/ABS190315026D",
pages = "443-453"
}

Dragićević, M., Ćuković, K., Zdravković-Korać, S., Simonović, A., Bogdanović, M.,& Todorović, S.. (2019). Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. in Archives of Biological Sciences, 71(3), 443-453.
https://doi.org/10.2298/ABS190315026D

Dragićević M, Ćuković K, Zdravković-Korać S, Simonović A, Bogdanović M, Todorović S. Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. in Archives of Biological Sciences. 2019;71(3):443-453.
doi:10.2298/ABS190315026D .

Dragićević, Milan, Ćuković, Katarina, Zdravković-Korać, Snežana, Simonović, Ana, Bogdanović, Milica, Todorović, Slađana, "Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach" in Archives of Biological Sciences, 71, no. 3 (2019):443-453,
https://doi.org/10.2298/ABS190315026D . .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Karličić, Vera
AU  - Raičević, Vera
AU  - Ćirković, Saša
AU  - Jasna, Ristić-Đurović
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4330
AB  - Flax (Linum usitatissimum) is an important food and fiber crop, due to its nutritional and nutraceutical qualities. Flax seeds have been consumed for at least 6,000 years, which makes it one of
the world’s first cultivated crops. Seed priming with living bacterial inoculum, termed biopriming,
involves the application of plant growth-promoting rhizobacteria. The present study was conducted to investigate the effect of growth-promoting rhizobacterium Bacillus amyloliquefaciens
on the growth of flax seedlings under different strengths of magnetic field. Seedlings were soaked
in bacterial suspension and bacteria were allowed to colonize. After ten minutes of soaking, the
seedlings were exposed to magnetic field of 60 and 90 mT for 24 h, in dark under laboratory conditions. Untreated seedlings were used as controls. Shoot and root length, number and length of
newly formed lateral roots and biomass production were measured after 1, 3, 5, 7 and 10 days. Results indicate that the combinations of magnetic field and plant growth-promoting bacteria gave
half- to two-fold better results than bacteria or magnetic field alone. The best result was achieved
in seedlings treated with Bacillus amyloliquefaciens and 90 mT magnetic field. This work showed a
significant impact of bio- and magneto-priming on the improvement of flax seedling growth and
biomass productivity.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Influence of bio- and magneto-priming on flax seedlings growth
SP  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4330
ER  - 

@conference{
author = "Ćalić, Dušica and Devrnja, Nina and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana and Karličić, Vera and Raičević, Vera and Ćirković, Saša and Jasna, Ristić-Đurović",
year = "2018",
abstract = "Flax (Linum usitatissimum) is an important food and fiber crop, due to its nutritional and nutraceutical qualities. Flax seeds have been consumed for at least 6,000 years, which makes it one of
the world’s first cultivated crops. Seed priming with living bacterial inoculum, termed biopriming,
involves the application of plant growth-promoting rhizobacteria. The present study was conducted to investigate the effect of growth-promoting rhizobacterium Bacillus amyloliquefaciens
on the growth of flax seedlings under different strengths of magnetic field. Seedlings were soaked
in bacterial suspension and bacteria were allowed to colonize. After ten minutes of soaking, the
seedlings were exposed to magnetic field of 60 and 90 mT for 24 h, in dark under laboratory conditions. Untreated seedlings were used as controls. Shoot and root length, number and length of
newly formed lateral roots and biomass production were measured after 1, 3, 5, 7 and 10 days. Results indicate that the combinations of magnetic field and plant growth-promoting bacteria gave
half- to two-fold better results than bacteria or magnetic field alone. The best result was achieved
in seedlings treated with Bacillus amyloliquefaciens and 90 mT magnetic field. This work showed a
significant impact of bio- and magneto-priming on the improvement of flax seedling growth and
biomass productivity.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Influence of bio- and magneto-priming on flax seedlings growth",
pages = "140-141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4330"
}

Ćalić, D., Devrnja, N., Milojević, J., Belić, M., Zdravković-Korać, S., Karličić, V., Raičević, V., Ćirković, S.,& Jasna, R.. (2018). Influence of bio- and magneto-priming on flax seedlings growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 140-141.
https://hdl.handle.net/21.15107/rcub_ibiss_4330

Ćalić D, Devrnja N, Milojević J, Belić M, Zdravković-Korać S, Karličić V, Raičević V, Ćirković S, Jasna R. Influence of bio- and magneto-priming on flax seedlings growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:140-141.
https://hdl.handle.net/21.15107/rcub_ibiss_4330 .

Ćalić, Dušica, Devrnja, Nina, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, Karličić, Vera, Raičević, Vera, Ćirković, Saša, Jasna, Ristić-Đurović, "Influence of bio- and magneto-priming on flax seedlings growth" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):140-141,
https://hdl.handle.net/21.15107/rcub_ibiss_4330 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Belić, Maja
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4327
AB  - Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4327
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Devrnja, Nina and Belić, Maja and Milojević, Jelena",
year = "2018",
abstract = "Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4327"
}

Zdravković-Korać, S., Ćalić, D., Devrnja, N., Belić, M.,& Milojević, J.. (2018). Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327

Zdravković-Korać S, Ćalić D, Devrnja N, Belić M, Milojević J. Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Devrnja, Nina, Belić, Maja, Milojević, Jelena, "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):38,
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .