TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Parabucki, Ana
AU  - Dacic, Sanja
AU  - Laketa, Danijela
AU  - Peković, Sanja
AU  - Stojiljkovic, Mirjana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2062
AB  - Compelling evidence now points to the critical role of the cytoskeleton
   in neurodegeneration. In the present study, using an immunohistochemical
   approach, we have shown that cortical stab injury (CSI) in adult Wistar
   rats significantly affects temporal pattern of expression of
   neurofilament proteins (NFs). a major cytoskeleton components of
   neurons, and microtubule-associated proteins (MAP2). At 3 days
   post-injury (dpi) most of the NFs immunoreactivity was found in pyknotic
   neurons and in fragmentized axonal processes in the perilesioned cortex.
   These cytoskeletal alterations became more pronounced by 10 dpi. At the
   subcellular level CSI also showed significant impact on NFs and MAP-2
   expression. Thus, at 3 dpi most of the dendrites disappeared, while
   large neuronal somata appeared like open circles pointing to membrane
   disintegration. Conversely, at 10 dpi neuronal perikarya and a few new
   apical dendrites were strongly labeled. Since aberrant NF
   phosphorylation is a pathological hallmark of many human
   neurodegenerative disorders, as well as is found after stressor stimuli,
   the present results shed light into the expression of neurofilaments
   after the stab brain injury. (C) 2014 Elsevier GmbH. All rights
   reserved.
T2  - Acta Histochemica
T1  - Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study
IS  - 2
VL  - 117
DO  - 10.1016/j.acthis.2014.12.004
SP  - 155
EP  - 162
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Parabucki, Ana and Dacic, Sanja and Laketa, Danijela and Peković, Sanja and Stojiljkovic, Mirjana",
year = "2015",
abstract = "Compelling evidence now points to the critical role of the cytoskeleton
   in neurodegeneration. In the present study, using an immunohistochemical
   approach, we have shown that cortical stab injury (CSI) in adult Wistar
   rats significantly affects temporal pattern of expression of
   neurofilament proteins (NFs). a major cytoskeleton components of
   neurons, and microtubule-associated proteins (MAP2). At 3 days
   post-injury (dpi) most of the NFs immunoreactivity was found in pyknotic
   neurons and in fragmentized axonal processes in the perilesioned cortex.
   These cytoskeletal alterations became more pronounced by 10 dpi. At the
   subcellular level CSI also showed significant impact on NFs and MAP-2
   expression. Thus, at 3 dpi most of the dendrites disappeared, while
   large neuronal somata appeared like open circles pointing to membrane
   disintegration. Conversely, at 10 dpi neuronal perikarya and a few new
   apical dendrites were strongly labeled. Since aberrant NF
   phosphorylation is a pathological hallmark of many human
   neurodegenerative disorders, as well as is found after stressor stimuli,
   the present results shed light into the expression of neurofilaments
   after the stab brain injury. (C) 2014 Elsevier GmbH. All rights
   reserved.",
journal = "Acta Histochemica",
title = "Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study",
number = "2",
volume = "117",
doi = "10.1016/j.acthis.2014.12.004",
pages = "155-162"
}

Lavrnja, I., Savić, D., Parabucki, A., Dacic, S., Laketa, D., Peković, S.,& Stojiljkovic, M.. (2015). Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study. in Acta Histochemica, 117(2), 155-162.
https://doi.org/10.1016/j.acthis.2014.12.004

Lavrnja I, Savić D, Parabucki A, Dacic S, Laketa D, Peković S, Stojiljkovic M. Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study. in Acta Histochemica. 2015;117(2):155-162.
doi:10.1016/j.acthis.2014.12.004 .

Lavrnja, Irena, Savić, Danijela, Parabucki, Ana, Dacic, Sanja, Laketa, Danijela, Peković, Sanja, Stojiljkovic, Mirjana, "Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study" in Acta Histochemica, 117, no. 2 (2015):155-162,
https://doi.org/10.1016/j.acthis.2014.12.004 . .

TY  - JOUR
AU  - Laketa, Danijela
AU  - Savić, Jasmina
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Vasić, Vesna
AU  - Stojiljković, Mirjana
AU  - Nedeljković, Nadežda
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2093
AB  - Background: Cortical stab injury (CSI) induces changes in the activity,
   expression and cellular distribution of specific ectonucleotidases at
   the injury site. Also, several experimentally induced neuropathologies
   are associated with changes in soluble ectonucleotidase activities in
   the plasma and serum, whilst various insults to the brain alter purine
   compounds levels in cerebrospinal fluid, but also in serum, indicating
   that insults to the brain may induce alterations in nucleotides release
   and rate of their hydrolysis in the vascular system. Since adenine
   nucleotides and adenosine regulate diverse cellular functions in the
   vascular system, including vascular tone, platelet aggregation and
   inflammatory responses of lymphocytes and macrophages, alterations of
   ectonucleotidase activities in the vascular system may be relevant for
   the clinical outcome of the primary insult.
   Methods: We explored ectonucleotidase activities using specific enzyme
   assays and determined adenine nucleotides concentrations by the UPLC
   method in the rat serum after cortical stab injury.
   Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about
   60\% and 40\%, respectively, while phosphodiesterase activity remained
   unchanged. Also, at 4-h postinjury a marked decrease in ATP
   concentration and more than 2-fold increase in AMP concentration were
   recorded.
   Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing
   soluble ectonucleotidases in rat serum, which leads to the observed
   shift in serum nucleotide levels. The results obtained imply that
   ectonucleotidases and adenine nucleotides participate in the
   communication between the brain and the vascular system in physiological
   and pathological conditions and thereby may be involved in the
   development of various human neuropathologies.
T2  - Journal of Medical Biochemistry
T1  - BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM
IS  - 2
VL  - 34
DO  - 10.2478/jomb-2014-0025
SP  - 215
EP  - 222
ER  - 

@article{
author = "Laketa, Danijela and Savić, Jasmina and Bjelobaba, Ivana and Lavrnja, Irena and Vasić, Vesna and Stojiljković, Mirjana and Nedeljković, Nadežda",
year = "2015",
abstract = "Background: Cortical stab injury (CSI) induces changes in the activity,
   expression and cellular distribution of specific ectonucleotidases at
   the injury site. Also, several experimentally induced neuropathologies
   are associated with changes in soluble ectonucleotidase activities in
   the plasma and serum, whilst various insults to the brain alter purine
   compounds levels in cerebrospinal fluid, but also in serum, indicating
   that insults to the brain may induce alterations in nucleotides release
   and rate of their hydrolysis in the vascular system. Since adenine
   nucleotides and adenosine regulate diverse cellular functions in the
   vascular system, including vascular tone, platelet aggregation and
   inflammatory responses of lymphocytes and macrophages, alterations of
   ectonucleotidase activities in the vascular system may be relevant for
   the clinical outcome of the primary insult.
   Methods: We explored ectonucleotidase activities using specific enzyme
   assays and determined adenine nucleotides concentrations by the UPLC
   method in the rat serum after cortical stab injury.
   Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about
   60\% and 40\%, respectively, while phosphodiesterase activity remained
   unchanged. Also, at 4-h postinjury a marked decrease in ATP
   concentration and more than 2-fold increase in AMP concentration were
   recorded.
   Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing
   soluble ectonucleotidases in rat serum, which leads to the observed
   shift in serum nucleotide levels. The results obtained imply that
   ectonucleotidases and adenine nucleotides participate in the
   communication between the brain and the vascular system in physiological
   and pathological conditions and thereby may be involved in the
   development of various human neuropathologies.",
journal = "Journal of Medical Biochemistry",
title = "BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM",
number = "2",
volume = "34",
doi = "10.2478/jomb-2014-0025",
pages = "215-222"
}

Laketa, D., Savić, J., Bjelobaba, I., Lavrnja, I., Vasić, V., Stojiljković, M.,& Nedeljković, N.. (2015). BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM. in Journal of Medical Biochemistry, 34(2), 215-222.
https://doi.org/10.2478/jomb-2014-0025

Laketa D, Savić J, Bjelobaba I, Lavrnja I, Vasić V, Stojiljković M, Nedeljković N. BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM. in Journal of Medical Biochemistry. 2015;34(2):215-222.
doi:10.2478/jomb-2014-0025 .

Laketa, Danijela, Savić, Jasmina, Bjelobaba, Ivana, Lavrnja, Irena, Vasić, Vesna, Stojiljković, Mirjana, Nedeljković, Nadežda, "BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM" in Journal of Medical Biochemistry, 34, no. 2 (2015):215-222,
https://doi.org/10.2478/jomb-2014-0025 . .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Parabucki, Ana
AU  - Brkić, Predrag
AU  - Jovanović, Tomislav
AU  - Dacic, Sanja
AU  - Savić, Danijela
AU  - Pantić, Igor
AU  - Stojiljković, Mirjana
AU  - Peković, Sanja
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2070
AB  - The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
   exerts its beneficial effects on recovery after brain injury are still
   unrevealed. Therefore, in this study we investigated the influence of
   repetitive HBOT on the reactive astrogliosis and expression of mediators
   of inflammation after cortical stab injury (CSI). CSI was performed on
   male Wistar rats, divided into control, sham, and lesioned groups with
   appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
   compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
   minutes of slow decompression, once a day for 10 consecutive days. Data
   obtained using real-time polymerase chain reaction, Western blot, and
   immunohistochemical and immunofluorescence analyses revealed that
   repetitive HBOT applied after the CSI attenuates reactive astrogliosis
   and glial scarring, and reduces expression of GFAP (glial fibrillary
   acidic protein), vimentin, and ICAM-1 (intercellular adhesion
   molecule-1) both at gene and tissue levels. In addition, HBOT prevents
   expression of CD40 and its ligand CD40L on microglia, neutrophils,
   cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
   by prevention of glial scarring and limiting of expression of
   inflammatory mediators, supports formation of more permissive
   environment for repair and regeneration.
T2  - Mediators of Inflammation
T1  - Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury
IS  - 498405
DO  - 10.1155/2015/498405
ER  - 

@article{
author = "Lavrnja, Irena and Parabucki, Ana and Brkić, Predrag and Jovanović, Tomislav and Dacic, Sanja and Savić, Danijela and Pantić, Igor and Stojiljković, Mirjana and Peković, Sanja",
year = "2015",
abstract = "The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
   exerts its beneficial effects on recovery after brain injury are still
   unrevealed. Therefore, in this study we investigated the influence of
   repetitive HBOT on the reactive astrogliosis and expression of mediators
   of inflammation after cortical stab injury (CSI). CSI was performed on
   male Wistar rats, divided into control, sham, and lesioned groups with
   appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
   compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
   minutes of slow decompression, once a day for 10 consecutive days. Data
   obtained using real-time polymerase chain reaction, Western blot, and
   immunohistochemical and immunofluorescence analyses revealed that
   repetitive HBOT applied after the CSI attenuates reactive astrogliosis
   and glial scarring, and reduces expression of GFAP (glial fibrillary
   acidic protein), vimentin, and ICAM-1 (intercellular adhesion
   molecule-1) both at gene and tissue levels. In addition, HBOT prevents
   expression of CD40 and its ligand CD40L on microglia, neutrophils,
   cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
   by prevention of glial scarring and limiting of expression of
   inflammatory mediators, supports formation of more permissive
   environment for repair and regeneration.",
journal = "Mediators of Inflammation",
title = "Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury",
number = "498405",
doi = "10.1155/2015/498405"
}

Lavrnja, I., Parabucki, A., Brkić, P., Jovanović, T., Dacic, S., Savić, D., Pantić, I., Stojiljković, M.,& Peković, S.. (2015). Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury. in Mediators of Inflammation(498405).
https://doi.org/10.1155/2015/498405

Lavrnja I, Parabucki A, Brkić P, Jovanović T, Dacic S, Savić D, Pantić I, Stojiljković M, Peković S. Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury. in Mediators of Inflammation. 2015;(498405).
doi:10.1155/2015/498405 .

Lavrnja, Irena, Parabucki, Ana, Brkić, Predrag, Jovanović, Tomislav, Dacic, Sanja, Savić, Danijela, Pantić, Igor, Stojiljković, Mirjana, Peković, Sanja, "Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury" in Mediators of Inflammation, no. 498405 (2015),
https://doi.org/10.1155/2015/498405 . .

TY  - JOUR
AU  - Božić, Iva
AU  - Savić, Danijela
AU  - Jovanović, Marija
AU  - Bjelobaba, Ivana
AU  - Laketa, Danijela
AU  - Nedeljković, Nadežda
AU  - Stojiljković, Mirjana
AU  - Peković, Sanja
AU  - Lavrnja, Irena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2047
AB  - Microglia play a key role in defending central nervous system from
   various internal and external threats. However, their excessive and/or
   chronic activation is associated with deleterious effects in a variety
   of neurodegenerative diseases. Previously, we have shown that ribavirin
   when applied in clinically relevant dosage (10 mu M) modulates activated
   microglia in complex fashion inducing both anti-and proinflammatory
   effects, simultaneously causing cytotoxicity. Here, we examined
   potential of low-dose ribavirin (0.1 and 1 mu M) to modulate activated
   BV-2 microglia. Morphological and functional activation of BV-2 cells
   was achieved with lipopolysaccharide (LPS) stimulation. Our results
   demonstrated that low-dose ribavirin did not induce cell death, while 10
   mu M ribavirin promoted LPS induced apoptosis. We determined that 1 mu M
   ribavirin was equally efficient in deactivation of LPS induced
   morphological changes as 10 mu M ribavirin treatment. Ribavirin showed
   halfway success in reducing markers of functional activation of
   microglia. Namely, none of the doses had effect on LPS triggered
   production of proinflammatory cytokine tumor necrosis factor alpha. On
   the other hand, low-dose ribavirin proved its effectiveness in reduction
   of another inflammatory mediator, nitric oxide, by inhibiting inducible
   form of nitric oxide synthase. Our results imply that low-dose ribavirin
   may alleviate nitrosative stress during neuroinflammation.
T2  - Analytical Cellular Pathology
T1  - Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase
IS  - 923614
DO  - 10.1155/2015/923614
ER  - 

@article{
author = "Božić, Iva and Savić, Danijela and Jovanović, Marija and Bjelobaba, Ivana and Laketa, Danijela and Nedeljković, Nadežda and Stojiljković, Mirjana and Peković, Sanja and Lavrnja, Irena",
year = "2015",
abstract = "Microglia play a key role in defending central nervous system from
   various internal and external threats. However, their excessive and/or
   chronic activation is associated with deleterious effects in a variety
   of neurodegenerative diseases. Previously, we have shown that ribavirin
   when applied in clinically relevant dosage (10 mu M) modulates activated
   microglia in complex fashion inducing both anti-and proinflammatory
   effects, simultaneously causing cytotoxicity. Here, we examined
   potential of low-dose ribavirin (0.1 and 1 mu M) to modulate activated
   BV-2 microglia. Morphological and functional activation of BV-2 cells
   was achieved with lipopolysaccharide (LPS) stimulation. Our results
   demonstrated that low-dose ribavirin did not induce cell death, while 10
   mu M ribavirin promoted LPS induced apoptosis. We determined that 1 mu M
   ribavirin was equally efficient in deactivation of LPS induced
   morphological changes as 10 mu M ribavirin treatment. Ribavirin showed
   halfway success in reducing markers of functional activation of
   microglia. Namely, none of the doses had effect on LPS triggered
   production of proinflammatory cytokine tumor necrosis factor alpha. On
   the other hand, low-dose ribavirin proved its effectiveness in reduction
   of another inflammatory mediator, nitric oxide, by inhibiting inducible
   form of nitric oxide synthase. Our results imply that low-dose ribavirin
   may alleviate nitrosative stress during neuroinflammation.",
journal = "Analytical Cellular Pathology",
title = "Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase",
number = "923614",
doi = "10.1155/2015/923614"
}

Božić, I., Savić, D., Jovanović, M., Bjelobaba, I., Laketa, D., Nedeljković, N., Stojiljković, M., Peković, S.,& Lavrnja, I.. (2015). Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase. in Analytical Cellular Pathology(923614).
https://doi.org/10.1155/2015/923614

Božić I, Savić D, Jovanović M, Bjelobaba I, Laketa D, Nedeljković N, Stojiljković M, Peković S, Lavrnja I. Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase. in Analytical Cellular Pathology. 2015;(923614).
doi:10.1155/2015/923614 .

Božić, Iva, Savić, Danijela, Jovanović, Marija, Bjelobaba, Ivana, Laketa, Danijela, Nedeljković, Nadežda, Stojiljković, Mirjana, Peković, Sanja, Lavrnja, Irena, "Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase" in Analytical Cellular Pathology, no. 923614 (2015),
https://doi.org/10.1155/2015/923614 . .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Peković, Sanja
AU  - Bjelobaba, Ivana
AU  - Stojiljkovic, Mirjana
AU  - Milošević, Verica
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2245
AB  - Traumatic brain injury (TBI) represents a serious event with far
   reaching complications, including pituitary dysfunction. Pars distalis
   corticotropes (ACTH cells), that represent the active module of
   hypothalamo-pituitary-adrenocortical axis, seem to be affected as well.
   Since pituitary failure after TBI has been associated with
   neurobehavioral impairments the aim of this study was to evaluate the
   effects of TBI on recovery of motor functions, morphology and secretory
   activity of ACTH cells in the pituitary of adult rats. Wistar male rats,
   initially exposed to sensorimotor cortex ablation (SCA), were sacrificed
   at the 2nd, 7th, 14th and 30th days post-surgery (dps). A beam walking
   test was used to evaluate the recovery of motor functions. Pituitary
   glands and blood were collected for morphological and hormonal analyses.
   During the first two weeks post-injury increased recovery of locomotor
   function was detected, reaching almost the control value at day 30. SCA
   induces significant increase of pituitary weights compared to their
   time-matched controls. The volume of ACTH-immunopositive cells was
   reduced at the 7th dps, while at the 14th dps their volume was enlarged,
   in comparison to corresponding sham controls. Volume density of ACTH
   cells was increased only at 14th dps, while at day 30 this increase was
   insignificant. The plasma level of ACTH transiently increased after the
   injury. The most pronounced changes were observed at the 7th and 14th
   dps, and were followed by decrease toward control levels at the 30th
   dps. Thus, temporal changes in the hypothalamic-pituitary-adrenal axis
   after traumatic brain injury appear to correlate with the recovery
   process. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Physiology & Behavior
T1  - Sensorimotor cortex ablation induces time-dependent response of ACTH
 cells in adult rats: Behavioral, immunohistomorphometric and hormonal
 study
VL  - 125
DO  - 10.1016/j.physbeh.2013.11.005
SP  - 30
EP  - 37
ER  - 

@article{
author = "Lavrnja, Irena and Trifunović, Svetlana and Ajdžanović, Vladimir and Peković, Sanja and Bjelobaba, Ivana and Stojiljkovic, Mirjana and Milošević, Verica",
year = "2014",
abstract = "Traumatic brain injury (TBI) represents a serious event with far
   reaching complications, including pituitary dysfunction. Pars distalis
   corticotropes (ACTH cells), that represent the active module of
   hypothalamo-pituitary-adrenocortical axis, seem to be affected as well.
   Since pituitary failure after TBI has been associated with
   neurobehavioral impairments the aim of this study was to evaluate the
   effects of TBI on recovery of motor functions, morphology and secretory
   activity of ACTH cells in the pituitary of adult rats. Wistar male rats,
   initially exposed to sensorimotor cortex ablation (SCA), were sacrificed
   at the 2nd, 7th, 14th and 30th days post-surgery (dps). A beam walking
   test was used to evaluate the recovery of motor functions. Pituitary
   glands and blood were collected for morphological and hormonal analyses.
   During the first two weeks post-injury increased recovery of locomotor
   function was detected, reaching almost the control value at day 30. SCA
   induces significant increase of pituitary weights compared to their
   time-matched controls. The volume of ACTH-immunopositive cells was
   reduced at the 7th dps, while at the 14th dps their volume was enlarged,
   in comparison to corresponding sham controls. Volume density of ACTH
   cells was increased only at 14th dps, while at day 30 this increase was
   insignificant. The plasma level of ACTH transiently increased after the
   injury. The most pronounced changes were observed at the 7th and 14th
   dps, and were followed by decrease toward control levels at the 30th
   dps. Thus, temporal changes in the hypothalamic-pituitary-adrenal axis
   after traumatic brain injury appear to correlate with the recovery
   process. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Physiology & Behavior",
title = "Sensorimotor cortex ablation induces time-dependent response of ACTH
 cells in adult rats: Behavioral, immunohistomorphometric and hormonal
 study",
volume = "125",
doi = "10.1016/j.physbeh.2013.11.005",
pages = "30-37"
}

Lavrnja, I., Trifunović, S., Ajdžanović, V., Peković, S., Bjelobaba, I., Stojiljkovic, M.,& Milošević, V.. (2014). Sensorimotor cortex ablation induces time-dependent response of ACTH
 cells in adult rats: Behavioral, immunohistomorphometric and hormonal
 study. in Physiology & Behavior, 125, 30-37.
https://doi.org/10.1016/j.physbeh.2013.11.005

Lavrnja I, Trifunović S, Ajdžanović V, Peković S, Bjelobaba I, Stojiljkovic M, Milošević V. Sensorimotor cortex ablation induces time-dependent response of ACTH
 cells in adult rats: Behavioral, immunohistomorphometric and hormonal
 study. in Physiology & Behavior. 2014;125:30-37.
doi:10.1016/j.physbeh.2013.11.005 .

Lavrnja, Irena, Trifunović, Svetlana, Ajdžanović, Vladimir, Peković, Sanja, Bjelobaba, Ivana, Stojiljkovic, Mirjana, Milošević, Verica, "Sensorimotor cortex ablation induces time-dependent response of ACTH
 cells in adult rats: Behavioral, immunohistomorphometric and hormonal
 study" in Physiology & Behavior, 125 (2014):30-37,
https://doi.org/10.1016/j.physbeh.2013.11.005 . .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Savić, Danijela
AU  - Milošević, Verica
AU  - Stojiljkovic, Mirjana
AU  - Peković, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2159
AB  - The pituitary appears to be vulnerable to brain trauma, and its
   dysfunction is a common feature after traumatic brain injury. The role
   of pituitary growth hormone (GH) in brain repair after injury has been
   envisaged, but more studies must be performed to understand completely
   the importance of GH in these processes. Because some of the
   neuroprotective effects of GH are mediated directly through the GH
   receptor (GHR), we examined GHR expression in the rat cerebral cortex
   after sensorimotor cortex ablation. RT-PCR, immunohistochemistry, and
   double immunofluorescence had been performed to analyze the correlation
   between GHR expression in the injured cortex and activity of GH cells in
   the pituitary. Our results showed that the volume of GH-immunopositive
   cells was reduced at days 2 and 7 postsurgery (dps), and volume density
   of GH cells was significantly decreased at 14 dps, all compared with
   appropriate sham controls. At 30 dps all investigated parameters had
   returned to control level. In the injured cortex, GHR expression was
   transiently upregulated. Increased GHR immunoreactivity was observed in
   reactive astrocytes at 7 and particularly at 14 dps. In neuronal cells,
   an increase of GHR immunoreactivity was seen in neuronal cell bodies and
   well-defined primary dendrites at 14 and especially at 30 dps. The
   results presented here suggest that, during recovery from brain injury,
   changes in activity of pituitary GH cells result in upregulation of GHR
   that may have a role in neuronal arborization and glial proliferation in
   the injured cortex. (C) 2014 Wiley Periodicals, Inc.
T2  - Journal of Neuroscience Research
T1  - Cortical Ablation Induces Time-Dependent Changes in Rat Pituitary
 Somatotrophs and Upregulates Growth Hormone Receptor Expression in the
 Injured Cortex
IS  - 10
VL  - 92
DO  - 10.1002/jnr.23408
SP  - 1338
EP  - 1349
ER  - 

@article{
author = "Lavrnja, Irena and Ajdžanović, Vladimir and Trifunović, Svetlana and Savić, Danijela and Milošević, Verica and Stojiljkovic, Mirjana and Peković, Sanja",
year = "2014",
abstract = "The pituitary appears to be vulnerable to brain trauma, and its
   dysfunction is a common feature after traumatic brain injury. The role
   of pituitary growth hormone (GH) in brain repair after injury has been
   envisaged, but more studies must be performed to understand completely
   the importance of GH in these processes. Because some of the
   neuroprotective effects of GH are mediated directly through the GH
   receptor (GHR), we examined GHR expression in the rat cerebral cortex
   after sensorimotor cortex ablation. RT-PCR, immunohistochemistry, and
   double immunofluorescence had been performed to analyze the correlation
   between GHR expression in the injured cortex and activity of GH cells in
   the pituitary. Our results showed that the volume of GH-immunopositive
   cells was reduced at days 2 and 7 postsurgery (dps), and volume density
   of GH cells was significantly decreased at 14 dps, all compared with
   appropriate sham controls. At 30 dps all investigated parameters had
   returned to control level. In the injured cortex, GHR expression was
   transiently upregulated. Increased GHR immunoreactivity was observed in
   reactive astrocytes at 7 and particularly at 14 dps. In neuronal cells,
   an increase of GHR immunoreactivity was seen in neuronal cell bodies and
   well-defined primary dendrites at 14 and especially at 30 dps. The
   results presented here suggest that, during recovery from brain injury,
   changes in activity of pituitary GH cells result in upregulation of GHR
   that may have a role in neuronal arborization and glial proliferation in
   the injured cortex. (C) 2014 Wiley Periodicals, Inc.",
journal = "Journal of Neuroscience Research",
title = "Cortical Ablation Induces Time-Dependent Changes in Rat Pituitary
 Somatotrophs and Upregulates Growth Hormone Receptor Expression in the
 Injured Cortex",
number = "10",
volume = "92",
doi = "10.1002/jnr.23408",
pages = "1338-1349"
}

Lavrnja, I., Ajdžanović, V., Trifunović, S., Savić, D., Milošević, V., Stojiljkovic, M.,& Peković, S.. (2014). Cortical Ablation Induces Time-Dependent Changes in Rat Pituitary
 Somatotrophs and Upregulates Growth Hormone Receptor Expression in the
 Injured Cortex. in Journal of Neuroscience Research, 92(10), 1338-1349.
https://doi.org/10.1002/jnr.23408

Lavrnja I, Ajdžanović V, Trifunović S, Savić D, Milošević V, Stojiljkovic M, Peković S. Cortical Ablation Induces Time-Dependent Changes in Rat Pituitary
 Somatotrophs and Upregulates Growth Hormone Receptor Expression in the
 Injured Cortex. in Journal of Neuroscience Research. 2014;92(10):1338-1349.
doi:10.1002/jnr.23408 .

Lavrnja, Irena, Ajdžanović, Vladimir, Trifunović, Svetlana, Savić, Danijela, Milošević, Verica, Stojiljkovic, Mirjana, Peković, Sanja, "Cortical Ablation Induces Time-Dependent Changes in Rat Pituitary
 Somatotrophs and Upregulates Growth Hormone Receptor Expression in the
 Injured Cortex" in Journal of Neuroscience Research, 92, no. 10 (2014):1338-1349,
https://doi.org/10.1002/jnr.23408 . .

TY  - JOUR
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Dacic, Sanja
AU  - Bjelobaba, Ivana
AU  - Nedeljkovic, Nadezda
AU  - Stojiljkovic, Mirjana
AU  - Herdegen, Thomas
AU  - Peković, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2263
AB  - Tiazofurin is a purine nucleoside analogue, with a broad spectrum of
   antitumoral and anti-inflammatory properties. In the present study, we
   have investigated the effect of tiazofurin on microglial inflammatory
   response to lipopolysaccharide in vitro. The cytotoxic effect of the
   drug was examined by sulforhodamine B assay. The Griess method was used
   to quantify nitrite production. Microglial morphology was assessed by
   measuring cell body size. Release of the pro-inflammatory cytokines,
   tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and the
   anti-inflammatory cytokine interleukin-10, were evaluated by
   enzyme-linked immunosorbent assay. Our data showed that tiazofurin
   decreased the number of activated microglia, lowered nitric oxide
   production and reduced the average cell surface of these cells.
   Tiazofurin reduced tumor necrosis factor-alpha, interleukin-6 and
   increased interleukin-10 secretion. Conversely, this drug promoted the
   release of interleukin-1 beta. Results obtained in this study indicate
   that TR displayed both anti-and pro-inflammatory modulation of activated
   microglia that could be relevant for its antitumor action within the
   central nervous system.
T2  - Archives of Biological Sciences
T1  - Tiazofurin modulates lipopolysaccharide-activated microglia in vitro
IS  - 4
VL  - 66
DO  - 10.2298/ABS1404633S
SP  - 1633
EP  - 1640
ER  - 

@article{
author = "Savić, Danijela and Lavrnja, Irena and Dacic, Sanja and Bjelobaba, Ivana and Nedeljkovic, Nadezda and Stojiljkovic, Mirjana and Herdegen, Thomas and Peković, Sanja",
year = "2014",
abstract = "Tiazofurin is a purine nucleoside analogue, with a broad spectrum of
   antitumoral and anti-inflammatory properties. In the present study, we
   have investigated the effect of tiazofurin on microglial inflammatory
   response to lipopolysaccharide in vitro. The cytotoxic effect of the
   drug was examined by sulforhodamine B assay. The Griess method was used
   to quantify nitrite production. Microglial morphology was assessed by
   measuring cell body size. Release of the pro-inflammatory cytokines,
   tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and the
   anti-inflammatory cytokine interleukin-10, were evaluated by
   enzyme-linked immunosorbent assay. Our data showed that tiazofurin
   decreased the number of activated microglia, lowered nitric oxide
   production and reduced the average cell surface of these cells.
   Tiazofurin reduced tumor necrosis factor-alpha, interleukin-6 and
   increased interleukin-10 secretion. Conversely, this drug promoted the
   release of interleukin-1 beta. Results obtained in this study indicate
   that TR displayed both anti-and pro-inflammatory modulation of activated
   microglia that could be relevant for its antitumor action within the
   central nervous system.",
journal = "Archives of Biological Sciences",
title = "Tiazofurin modulates lipopolysaccharide-activated microglia in vitro",
number = "4",
volume = "66",
doi = "10.2298/ABS1404633S",
pages = "1633-1640"
}

Savić, D., Lavrnja, I., Dacic, S., Bjelobaba, I., Nedeljkovic, N., Stojiljkovic, M., Herdegen, T.,& Peković, S.. (2014). Tiazofurin modulates lipopolysaccharide-activated microglia in vitro. in Archives of Biological Sciences, 66(4), 1633-1640.
https://doi.org/10.2298/ABS1404633S

Savić D, Lavrnja I, Dacic S, Bjelobaba I, Nedeljkovic N, Stojiljkovic M, Herdegen T, Peković S. Tiazofurin modulates lipopolysaccharide-activated microglia in vitro. in Archives of Biological Sciences. 2014;66(4):1633-1640.
doi:10.2298/ABS1404633S .

Savić, Danijela, Lavrnja, Irena, Dacic, Sanja, Bjelobaba, Ivana, Nedeljkovic, Nadezda, Stojiljkovic, Mirjana, Herdegen, Thomas, Peković, Sanja, "Tiazofurin modulates lipopolysaccharide-activated microglia in vitro" in Archives of Biological Sciences, 66, no. 4 (2014):1633-1640,
https://doi.org/10.2298/ABS1404633S . .

TY  - JOUR
AU  - Savić, Danijela
AU  - Stojiljkovic, Mirjana
AU  - Lavrnja, Irena
AU  - Parabucki, Ana
AU  - Bjelobaba, Ivana
AU  - Nedeljkovic, Nadezda
AU  - Herdegen, Thomas
AU  - Peković, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2120
AB  - Ribavirin (RBV) is synthetic purine nucleoside analogue, licensed as
   anti-viral drug that displays immunomodulatory actions on various immune
   cells. Our previous ex vivo studies have demonstrated immunosuppressive
   effects of RBV on reactive T-lymphocytes in experimental autoimmune
   encephalomyelitis. Here, we examined the effects of RBV on inflammatory
   response of microglia. RBV potency to down-regulate microglia
   inflammatory response was assessed by measuring microglia cell body
   size, and the production of nitric oxide (NO) and pro-and
   anti-inflammatory cytokines. RBV exerted cytotoxic effects on
   LPS-stimulated microglia, leaving non-stimulated microglia unaffected.
   The exposure of activated microglia to RBV led to: decrease in the level
   of NO as a result of decreased cell number, lower average cell surface,
   the reduction of membrane ruffling, the suppression of interleukin-6
   release and promoted interleukin-10 production. On the other hand, RBV
   promoted LPS-induced interleukin-1 beta release. Our results imply that
   RBV is a complex immunomodulator showing both anti-and pro-inflammatory
   effects on activated microglia.
T2  - Immunopharmacology and Immunotoxicology
T1  - Ribavirin shows immunomodulatory effects on activated microglia
IS  - 6
VL  - 36
DO  - 10.3109/08923973.2014.971962
SP  - 433
EP  - 441
ER  - 

@article{
author = "Savić, Danijela and Stojiljkovic, Mirjana and Lavrnja, Irena and Parabucki, Ana and Bjelobaba, Ivana and Nedeljkovic, Nadezda and Herdegen, Thomas and Peković, Sanja",
year = "2014",
abstract = "Ribavirin (RBV) is synthetic purine nucleoside analogue, licensed as
   anti-viral drug that displays immunomodulatory actions on various immune
   cells. Our previous ex vivo studies have demonstrated immunosuppressive
   effects of RBV on reactive T-lymphocytes in experimental autoimmune
   encephalomyelitis. Here, we examined the effects of RBV on inflammatory
   response of microglia. RBV potency to down-regulate microglia
   inflammatory response was assessed by measuring microglia cell body
   size, and the production of nitric oxide (NO) and pro-and
   anti-inflammatory cytokines. RBV exerted cytotoxic effects on
   LPS-stimulated microglia, leaving non-stimulated microglia unaffected.
   The exposure of activated microglia to RBV led to: decrease in the level
   of NO as a result of decreased cell number, lower average cell surface,
   the reduction of membrane ruffling, the suppression of interleukin-6
   release and promoted interleukin-10 production. On the other hand, RBV
   promoted LPS-induced interleukin-1 beta release. Our results imply that
   RBV is a complex immunomodulator showing both anti-and pro-inflammatory
   effects on activated microglia.",
journal = "Immunopharmacology and Immunotoxicology",
title = "Ribavirin shows immunomodulatory effects on activated microglia",
number = "6",
volume = "36",
doi = "10.3109/08923973.2014.971962",
pages = "433-441"
}

Savić, D., Stojiljkovic, M., Lavrnja, I., Parabucki, A., Bjelobaba, I., Nedeljkovic, N., Herdegen, T.,& Peković, S.. (2014). Ribavirin shows immunomodulatory effects on activated microglia. in Immunopharmacology and Immunotoxicology, 36(6), 433-441.
https://doi.org/10.3109/08923973.2014.971962

Savić D, Stojiljkovic M, Lavrnja I, Parabucki A, Bjelobaba I, Nedeljkovic N, Herdegen T, Peković S. Ribavirin shows immunomodulatory effects on activated microglia. in Immunopharmacology and Immunotoxicology. 2014;36(6):433-441.
doi:10.3109/08923973.2014.971962 .

Savić, Danijela, Stojiljkovic, Mirjana, Lavrnja, Irena, Parabucki, Ana, Bjelobaba, Ivana, Nedeljkovic, Nadezda, Herdegen, Thomas, Peković, Sanja, "Ribavirin shows immunomodulatory effects on activated microglia" in Immunopharmacology and Immunotoxicology, 36, no. 6 (2014):433-441,
https://doi.org/10.3109/08923973.2014.971962 . .

TY  - JOUR
AU  - Nedeljković, Nadežda
AU  - Bjelobaba, Ivana
AU  - Dacić, Sanja
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Savić, Danijela
AU  - Vještica, Aleksandar
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2006
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1016/j.cellbi.2006.03.001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3464
UR  - https://www.sciencedirect.com/science/article/abs/pii/S1065699506000643
AB  - The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-nificant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.
PB  - Elsevier
T2  - Cell Biology International
T1  - Up-regulation of ectonucleotidase activity after cortical stab injury in rats
IS  - 6
VL  - 30
DO  - 10.1016/j.cellbi.2006.03.001
SP  - 541
EP  - 546
ER  - 

@article{
author = "Nedeljković, Nadežda and Bjelobaba, Ivana and Dacić, Sanja and Lavrnja, Irena and Peković, Sanja and Savić, Danijela and Vještica, Aleksandar and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2006",
abstract = "The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-nificant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.",
publisher = "Elsevier",
journal = "Cell Biology International",
title = "Up-regulation of ectonucleotidase activity after cortical stab injury in rats",
number = "6",
volume = "30",
doi = "10.1016/j.cellbi.2006.03.001",
pages = "541-546"
}

Nedeljković, N., Bjelobaba, I., Dacić, S., Lavrnja, I., Peković, S., Savić, D., Vještica, A., Rakić, L.,& Stojiljković, M.. (2006). Up-regulation of ectonucleotidase activity after cortical stab injury in rats. in Cell Biology International
Elsevier., 30(6), 541-546.
https://doi.org/10.1016/j.cellbi.2006.03.001

Nedeljković N, Bjelobaba I, Dacić S, Lavrnja I, Peković S, Savić D, Vještica A, Rakić L, Stojiljković M. Up-regulation of ectonucleotidase activity after cortical stab injury in rats. in Cell Biology International. 2006;30(6):541-546.
doi:10.1016/j.cellbi.2006.03.001 .

Nedeljković, Nadežda, Bjelobaba, Ivana, Dacić, Sanja, Lavrnja, Irena, Peković, Sanja, Savić, Danijela, Vještica, Aleksandar, Rakić, Ljubisav, Stojiljković, Mirjana, "Up-regulation of ectonucleotidase activity after cortical stab injury in rats" in Cell Biology International, 30, no. 6 (2006):541-546,
https://doi.org/10.1016/j.cellbi.2006.03.001 . .