TY  - JOUR
AU  - Albuquerque, Bianca R.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Oliveira, M Beatriz P P
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996923001199
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5481
AB  - Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.
T2  - Food Research International
T1  - Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins
VL  - 165
DO  - 10.1016/j.foodres.2023.112574
SP  - 112574
ER  - 

@article{
author = "Albuquerque, Bianca R. and Pinela, José and Dias, Maria Inês and Pereira, Carla and Petrović, Jovana and Soković, Marina and Calhelha, Ricardo C. and Oliveira, M Beatriz P P and Ferreira, Isabel C F R and Barros, Lillian",
year = "2023",
abstract = "Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.",
journal = "Food Research International",
title = "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins",
volume = "165",
doi = "10.1016/j.foodres.2023.112574",
pages = "112574"
}

Albuquerque, B. R., Pinela, J., Dias, M. I., Pereira, C., Petrović, J., Soković, M., Calhelha, R. C., Oliveira, M. B. P. P., Ferreira, I. C. F. R.,& Barros, L.. (2023). Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International, 165, 112574.
https://doi.org/10.1016/j.foodres.2023.112574

Albuquerque BR, Pinela J, Dias MI, Pereira C, Petrović J, Soković M, Calhelha RC, Oliveira MBPP, Ferreira ICFR, Barros L. Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International. 2023;165:112574.
doi:10.1016/j.foodres.2023.112574 .

Albuquerque, Bianca R., Pinela, José, Dias, Maria Inês, Pereira, Carla, Petrović, Jovana, Soković, Marina, Calhelha, Ricardo C., Oliveira, M Beatriz P P, Ferreira, Isabel C F R, Barros, Lillian, "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins" in Food Research International, 165 (2023):112574,
https://doi.org/10.1016/j.foodres.2023.112574 . .

TY  - JOUR
AU  - Fernandes, Angela
AU  - Polyzos, Nikolaos
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6399
AB  - The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants
IS  - 7
VL  - 9
DO  - 10.3390/horticulturae9070838
SP  - 838
ER  - 

@article{
author = "Fernandes, Angela and Polyzos, Nikolaos and Mandim, Filipa and Pereira, Carla and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon",
year = "2023",
abstract = "The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants",
number = "7",
volume = "9",
doi = "10.3390/horticulturae9070838",
pages = "838"
}

Fernandes, A., Polyzos, N., Mandim, F., Pereira, C., Petrović, J., Soković, M.,& Petropoulos, S.. (2023). Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae
Basel: MDPI., 9(7), 838.
https://doi.org/10.3390/horticulturae9070838

Fernandes A, Polyzos N, Mandim F, Pereira C, Petrović J, Soković M, Petropoulos S. Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae. 2023;9(7):838.
doi:10.3390/horticulturae9070838 .

Fernandes, Angela, Polyzos, Nikolaos, Mandim, Filipa, Pereira, Carla, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants" in Horticulturae, 9, no. 7 (2023):838,
https://doi.org/10.3390/horticulturae9070838 . .

TY  - JOUR
AU  - da Silva, Tamires Barlati Vieira
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Mandim, Filipa
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Seixas, Flávio Augusto Vicente
AU  - Bracht, Adelar
AU  - Peralta, Rosane Marina
PY  - 2023
UR  - http://xlink.rsc.org/?DOI=D2FO02442J
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36723015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5465
AB  - A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.
T2  - Food & Function
T1  - Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice
IS  - 3
VL  - 14
DO  - 10.1039/d2fo02442j
SP  - 1761
EP  - 1772
ER  - 

@article{
author = "da Silva, Tamires Barlati Vieira and Dias, Maria Inês and Pereira, Carla and Mandim, Filipa and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian and Seixas, Flávio Augusto Vicente and Bracht, Adelar and Peralta, Rosane Marina",
year = "2023",
abstract = "A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.",
journal = "Food & Function",
title = "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice",
number = "3",
volume = "14",
doi = "10.1039/d2fo02442j",
pages = "1761-1772"
}

da Silva, T. B. V., Dias, M. I., Pereira, C., Mandim, F., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Barros, L., Seixas, F. A. V., Bracht, A.,& Peralta, R. M.. (2023). Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function, 14(3), 1761-1772.
https://doi.org/10.1039/d2fo02442j

da Silva TBV, Dias MI, Pereira C, Mandim F, Ivanov M, Soković M, Ferreira ICFR, Barros L, Seixas FAV, Bracht A, Peralta RM. Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function. 2023;14(3):1761-1772.
doi:10.1039/d2fo02442j .

da Silva, Tamires Barlati Vieira, Dias, Maria Inês, Pereira, Carla, Mandim, Filipa, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, Seixas, Flávio Augusto Vicente, Bracht, Adelar, Peralta, Rosane Marina, "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice" in Food & Function, 14, no. 3 (2023):1761-1772,
https://doi.org/10.1039/d2fo02442j . .

TY  - JOUR
AU  - Garcia-Oliveira, Paula
AU  - Carreira-Casais, Anxo
AU  - Pereira, Eliana
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Simal-Gandara, Jesus
AU  - Prieto, Miguel A
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9571014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5170
AB  - Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.
PB  - Basel: MDPI
T2  - Molecules
T1  - From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants
IS  - 19
VL  - 27
DO  - 10.3390/molecules27196495
ER  - 

@article{
author = "Garcia-Oliveira, Paula and Carreira-Casais, Anxo and Pereira, Eliana and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C and Stojković, Dejan and Soković, Marina and Simal-Gandara, Jesus and Prieto, Miguel A and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants",
number = "19",
volume = "27",
doi = "10.3390/molecules27196495"
}

Garcia-Oliveira, P., Carreira-Casais, A., Pereira, E., Dias, M. I., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Simal-Gandara, J., Prieto, M. A., Caleja, C.,& Barros, L.. (2022). From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules
Basel: MDPI., 27(19).
https://doi.org/10.3390/molecules27196495

Garcia-Oliveira P, Carreira-Casais A, Pereira E, Dias MI, Pereira C, Calhelha RC, Stojković D, Soković M, Simal-Gandara J, Prieto MA, Caleja C, Barros L. From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules. 2022;27(19).
doi:10.3390/molecules27196495 .

Garcia-Oliveira, Paula, Carreira-Casais, Anxo, Pereira, Eliana, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C, Stojković, Dejan, Soković, Marina, Simal-Gandara, Jesus, Prieto, Miguel A, Caleja, Cristina, Barros, Lillian, "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196495 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Chaski, Christina
AU  - Pereira, Carla
AU  - Kostić, Marina
AU  - Rouphael, Youssef
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Petropoulos, Spyridon A.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/645
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5090
AB  - The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070645
SP  - 645
ER  - 

@article{
author = "Fernandes, Ângela and Chaski, Christina and Pereira, Carla and Kostić, Marina and Rouphael, Youssef and Soković, Marina and Barros, Lillian and Petropoulos, Spyridon A.",
year = "2022",
abstract = "The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070645",
pages = "645"
}

Fernandes, Â., Chaski, C., Pereira, C., Kostić, M., Rouphael, Y., Soković, M., Barros, L.,& Petropoulos, S. A.. (2022). Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae
Basel: MDPI., 8(7), 645.
https://doi.org/10.3390/horticulturae8070645

Fernandes Â, Chaski C, Pereira C, Kostić M, Rouphael Y, Soković M, Barros L, Petropoulos SA. Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae. 2022;8(7):645.
doi:10.3390/horticulturae8070645 .

Fernandes, Ângela, Chaski, Christina, Pereira, Carla, Kostić, Marina, Rouphael, Youssef, Soković, Marina, Barros, Lillian, Petropoulos, Spyridon A., "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit" in Horticulturae, 8, no. 7 (2022):645,
https://doi.org/10.3390/horticulturae8070645 . .

TY  - JOUR
AU  - Besrour, Nermine
AU  - Oludemi, Taofiq
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Soković, Marina
AU  - Stojković, Dejan
AU  - Ferreira, Olga
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/4/677
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9031312
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4963
AB  - The cosmetic industry is constantly searching for bioactive ingredients, namely, those obtained from natural sources with environmentally friendly connotations and less toxic effects. A previous study of our research group optimized the extraction of phenolic compounds from Juglans regia by heat-assisted extraction. Due to its richness in different phenolic compounds, the present work aimed to develop a formulation containing J. regia leaf extract. The extract's antioxidant, anti-tyrosinase, antimicrobial, anti-inflammatory, wound healing, cytotoxicity, and photostability properties were evaluated. The extract was then incorporated into an O/W base cream, followed by characterization of the final formulation in terms of its antioxidant properties, phenolic composition, and stability over time and at different storage conditions. The most abundant compounds in the hydroethanolic extract were 3-O-caffeoylquinic acid (18.30 ± 0.04 mg/g), quercetin-O-pentoside (9.64 ± 0.06 mg/g), and quercetin 3-O-glucoside (6.70 ± 0.19 mg/g). Besides those, the extract presented antioxidant, anti-inflammatory, wound closure, and antibacterial effects against several skin pathogens. In addition, HaCaT cell viability was maintained up to 98% at 400 µg/mL. Within Proteus vulgaris-infected HaCaT cells, the extract also presented an over 40% bacterial mortality rate at its nontoxic concentration (200 µg/mL). After incorporating the extract, the obtained formulation presented a good physicochemical profile over time and at different storage conditions while also maintaining its antioxidant effect; as such, it can be considered stable for topical application. Future work to evaluate its performance in terms of skin permeation and detailed toxicological studies with a focus on regulatory requirements, involving skin irritation, eye irritation, genotoxicity, photo-irritation, and dermal absorption, should be conducted, as the prepared formulation demonstrated relevant properties that deserve to be further explored.
PB  - Basel: MDPI
T2  - Antioxidants (Basel, Switzerland)
T1  - Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.
IS  - 4
VL  - 11
DO  - 10.3390/antiox11040677
SP  - 677
ER  - 

@article{
author = "Besrour, Nermine and Oludemi, Taofiq and Mandim, Filipa and Pereira, Carla and Dias, Maria Inês and Soković, Marina and Stojković, Dejan and Ferreira, Olga and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2022",
abstract = "The cosmetic industry is constantly searching for bioactive ingredients, namely, those obtained from natural sources with environmentally friendly connotations and less toxic effects. A previous study of our research group optimized the extraction of phenolic compounds from Juglans regia by heat-assisted extraction. Due to its richness in different phenolic compounds, the present work aimed to develop a formulation containing J. regia leaf extract. The extract's antioxidant, anti-tyrosinase, antimicrobial, anti-inflammatory, wound healing, cytotoxicity, and photostability properties were evaluated. The extract was then incorporated into an O/W base cream, followed by characterization of the final formulation in terms of its antioxidant properties, phenolic composition, and stability over time and at different storage conditions. The most abundant compounds in the hydroethanolic extract were 3-O-caffeoylquinic acid (18.30 ± 0.04 mg/g), quercetin-O-pentoside (9.64 ± 0.06 mg/g), and quercetin 3-O-glucoside (6.70 ± 0.19 mg/g). Besides those, the extract presented antioxidant, anti-inflammatory, wound closure, and antibacterial effects against several skin pathogens. In addition, HaCaT cell viability was maintained up to 98% at 400 µg/mL. Within Proteus vulgaris-infected HaCaT cells, the extract also presented an over 40% bacterial mortality rate at its nontoxic concentration (200 µg/mL). After incorporating the extract, the obtained formulation presented a good physicochemical profile over time and at different storage conditions while also maintaining its antioxidant effect; as such, it can be considered stable for topical application. Future work to evaluate its performance in terms of skin permeation and detailed toxicological studies with a focus on regulatory requirements, involving skin irritation, eye irritation, genotoxicity, photo-irritation, and dermal absorption, should be conducted, as the prepared formulation demonstrated relevant properties that deserve to be further explored.",
publisher = "Basel: MDPI",
journal = "Antioxidants (Basel, Switzerland)",
title = "Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.",
number = "4",
volume = "11",
doi = "10.3390/antiox11040677",
pages = "677"
}

Besrour, N., Oludemi, T., Mandim, F., Pereira, C., Dias, M. I., Soković, M., Stojković, D., Ferreira, O., Ferreira, I. C. F. R.,& Barros, L.. (2022). Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.. in Antioxidants (Basel, Switzerland)
Basel: MDPI., 11(4), 677.
https://doi.org/10.3390/antiox11040677

Besrour N, Oludemi T, Mandim F, Pereira C, Dias MI, Soković M, Stojković D, Ferreira O, Ferreira ICFR, Barros L. Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.. in Antioxidants (Basel, Switzerland). 2022;11(4):677.
doi:10.3390/antiox11040677 .

Besrour, Nermine, Oludemi, Taofiq, Mandim, Filipa, Pereira, Carla, Dias, Maria Inês, Soković, Marina, Stojković, Dejan, Ferreira, Olga, Ferreira, Isabel C. F. R., Barros, Lillian, "Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development." in Antioxidants (Basel, Switzerland), 11, no. 4 (2022):677,
https://doi.org/10.3390/antiox11040677 . .

TY  - JOUR
AU  - Vieira, Dalila M.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Barros, Lillian
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barreiro, Maria Filomena
AU  - Ferreira, Isabel C.F.R.
AU  - Castro, Maria Cidalia R.
AU  - Rodrigues, Pedro V.
AU  - Machado, Ana Vera
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fft2.141
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5435
AB  - Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.
PB  - Hoboken: Wiley
T2  - Food Frontiers
T1  - Evaluation of plant extracts as an efficient source of additives for active food packaging
IS  - 3
VL  - 3
DO  - 10.1002/fft2.141
SP  - 480
EP  - 488
ER  - 

@article{
author = "Vieira, Dalila M. and Pereira, Carla and Calhelha, Ricardo C. and Barros, Lillian and Petrović, Jovana and Soković, Marina and Barreiro, Maria Filomena and Ferreira, Isabel C.F.R. and Castro, Maria Cidalia R. and Rodrigues, Pedro V. and Machado, Ana Vera",
year = "2022",
abstract = "Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.",
publisher = "Hoboken: Wiley",
journal = "Food Frontiers",
title = "Evaluation of plant extracts as an efficient source of additives for active food packaging",
number = "3",
volume = "3",
doi = "10.1002/fft2.141",
pages = "480-488"
}

Vieira, D. M., Pereira, C., Calhelha, R. C., Barros, L., Petrović, J., Soković, M., Barreiro, M. F., Ferreira, I. C.F.R., Castro, M. C. R., Rodrigues, P. V.,& Machado, A. V.. (2022). Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers
Hoboken: Wiley., 3(3), 480-488.
https://doi.org/10.1002/fft2.141

Vieira DM, Pereira C, Calhelha RC, Barros L, Petrović J, Soković M, Barreiro MF, Ferreira IC, Castro MCR, Rodrigues PV, Machado AV. Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers. 2022;3(3):480-488.
doi:10.1002/fft2.141 .

Vieira, Dalila M., Pereira, Carla, Calhelha, Ricardo C., Barros, Lillian, Petrović, Jovana, Soković, Marina, Barreiro, Maria Filomena, Ferreira, Isabel C.F.R., Castro, Maria Cidalia R., Rodrigues, Pedro V., Machado, Ana Vera, "Evaluation of plant extracts as an efficient source of additives for active food packaging" in Food Frontiers, 3, no. 3 (2022):480-488,
https://doi.org/10.1002/fft2.141 . .

TY  - JOUR
AU  - Silva, Ana Rita
AU  - Ayuso, Manuel
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - García, Pablo A.
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021011080
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4746
AB  - Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter
VL  - 176
DO  - 10.1016/j.indcrop.2021.114343
SP  - 114343
ER  - 

@article{
author = "Silva, Ana Rita and Ayuso, Manuel and Pereira, Carla and Dias, Maria Inês and Kostić, Marina and Calhelha, Ricardo C. and Soković, Marina and García, Pablo A. and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2022",
abstract = "Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter",
volume = "176",
doi = "10.1016/j.indcrop.2021.114343",
pages = "114343"
}

Silva, A. R., Ayuso, M., Pereira, C., Dias, M. I., Kostić, M., Calhelha, R. C., Soković, M., García, P. A., Ferreira, I. C.F.R.,& Barros, L.. (2022). Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 176, 114343.
https://doi.org/10.1016/j.indcrop.2021.114343

Silva AR, Ayuso M, Pereira C, Dias MI, Kostić M, Calhelha RC, Soković M, García PA, Ferreira IC, Barros L. Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products. 2022;176:114343.
doi:10.1016/j.indcrop.2021.114343 .

Silva, Ana Rita, Ayuso, Manuel, Pereira, Carla, Dias, Maria Inês, Kostić, Marina, Calhelha, Ricardo C., Soković, Marina, García, Pablo A., Ferreira, Isabel C.F.R., Barros, Lillian, "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter" in Industrial Crops and Products, 176 (2022):114343,
https://doi.org/10.1016/j.indcrop.2021.114343 . .

TY  - CONF
AU  - Albuquerque, Bianca
AU  - Inês Dias, Maria
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - P.P. Oliveira, M.  Beatriz
AU  - C.F.C. Ferreira, Isabel
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6574
AB  - Tropical fruits are widely appreciated for their sweet taste and generally known for their rich 
composition in bioactive compounds. However, the processing of this type of fruits can generate a large 
amount of non-edible and non-tradable by-products, consisting mainly in peels and seeds. These by products have already been described as having a greater amount of bioactive compounds than the 
edible parts; this fact has been increasing the interest of the scientific community as also of several 
industrial fields for the application of these by-products. 1
In this context, the rigid non-edible epicarp 
of Sicana odorifera (vell.) Naudin, a purple-black tropical fruit from Brazil, 2 was evaluated for the 
anthocyanin and non-anthocyanin phenolic composition by High-Performance Liquid Chromatography 
coupled to a diode-array detector and a mass spectrometer functioning by electrospray ionization 
(HPLC-DAD/ESI-MS). The bioactive potential was assessed thought several in vitro assays: the 
antioxidant activity was determined by thiobarbituric acid reactive substances assay (TBARS) and by the 
oxidative hemolysis inhibition assay (OxHLIA) and the antimicrobial activity was tested in four bacteria 
and four fungi strains using the microdilution method. 
S. odorifera epicarp hydroethanolic extract presented four phenolic compounds, namely two O glycosylated quercetin and kaempherol derivatives and two O-glycosylated anthocyanins, being the 
latest group the most abundant one with a total amount of 24±1 mg of anthocyanins/g of dry epicarp. 
Concerning its bioactive potential, S. odorifera hydroethanolic extract revealed considerable antioxidant 
activity (EC50 values of 48.2±0.5 and 27±1 µg/mL for TBARS and OxHLIA assays, respectively) and 
antimicrobial action against all bacteria and fungi strains evaluated (minimal inhibitory concentrations 
(MICs) ≤ 2.2 mg/mL). 
The results obtained allow to classify this tropical fruit epicarp as a potential source of bioactive 
compounds with great market value, since they can be applied in several industrial sectors, including 
the food and pharmaceutical industries.
PB  - Universidade da Madeira, Centro de Química da Madeira
C3  - XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal
T1  - By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6574
ER  - 

@conference{
author = "Albuquerque, Bianca and Inês Dias, Maria and Pereira, Carla and Petrović, Jovana and Soković, Marina and P.P. Oliveira, M.  Beatriz and C.F.C. Ferreira, Isabel and Barros, Lillian",
year = "2021",
abstract = "Tropical fruits are widely appreciated for their sweet taste and generally known for their rich 
composition in bioactive compounds. However, the processing of this type of fruits can generate a large 
amount of non-edible and non-tradable by-products, consisting mainly in peels and seeds. These by products have already been described as having a greater amount of bioactive compounds than the 
edible parts; this fact has been increasing the interest of the scientific community as also of several 
industrial fields for the application of these by-products. 1
In this context, the rigid non-edible epicarp 
of Sicana odorifera (vell.) Naudin, a purple-black tropical fruit from Brazil, 2 was evaluated for the 
anthocyanin and non-anthocyanin phenolic composition by High-Performance Liquid Chromatography 
coupled to a diode-array detector and a mass spectrometer functioning by electrospray ionization 
(HPLC-DAD/ESI-MS). The bioactive potential was assessed thought several in vitro assays: the 
antioxidant activity was determined by thiobarbituric acid reactive substances assay (TBARS) and by the 
oxidative hemolysis inhibition assay (OxHLIA) and the antimicrobial activity was tested in four bacteria 
and four fungi strains using the microdilution method. 
S. odorifera epicarp hydroethanolic extract presented four phenolic compounds, namely two O glycosylated quercetin and kaempherol derivatives and two O-glycosylated anthocyanins, being the 
latest group the most abundant one with a total amount of 24±1 mg of anthocyanins/g of dry epicarp. 
Concerning its bioactive potential, S. odorifera hydroethanolic extract revealed considerable antioxidant 
activity (EC50 values of 48.2±0.5 and 27±1 µg/mL for TBARS and OxHLIA assays, respectively) and 
antimicrobial action against all bacteria and fungi strains evaluated (minimal inhibitory concentrations 
(MICs) ≤ 2.2 mg/mL). 
The results obtained allow to classify this tropical fruit epicarp as a potential source of bioactive 
compounds with great market value, since they can be applied in several industrial sectors, including 
the food and pharmaceutical industries.",
publisher = "Universidade da Madeira, Centro de Química da Madeira",
journal = "XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal",
title = "By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6574"
}

Albuquerque, B., Inês Dias, M., Pereira, C., Petrović, J., Soković, M., P.P. Oliveira, M.  Beatriz, C.F.C. Ferreira, I.,& Barros, L.. (2021). By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.. in XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal
Universidade da Madeira, Centro de Química da Madeira., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_6574

Albuquerque B, Inês Dias M, Pereira C, Petrović J, Soković M, P.P. Oliveira, M.  Beatriz, C.F.C. Ferreira I, Barros L. By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.. in XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal. 2021;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_6574 .

Albuquerque, Bianca, Inês Dias, Maria, Pereira, Carla, Petrović, Jovana, Soković, Marina, P.P. Oliveira, M.  Beatriz, C.F.C. Ferreira, Isabel, Barros, Lillian, "By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study." in XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal (2021):81,
https://hdl.handle.net/21.15107/rcub_ibiss_6574 .

TY  - JOUR
AU  - Lima, Juliana França
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Steinmacher, Nádia Cristiane
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2073-4395/11/9/1873
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4490
AB  - Consumers have given preferences to food products that, in addition to the nutritional properties, also present bioactive characteristics with beneficial health effects. The use of Nonconventional Food Plants (NCFP) has been an asset for the food industry, not only due to its abundance but, also, because it does not compete with other vegetable matrices used for human consumption for its nutritional properties, chemical and bioactive potentiality. The present work aimed to study the granulometry and water absorption index of three seeds of unconventional food plants: niger, millet and birdseed, followed by their nutritional value, content in free sugars, fatty acids, organic acids, tocopherols and phenolic compounds by chromatographic methods and, also, the evaluation of the antioxidant, hepatotoxic and antimicrobial potential in their hydroethanolic extracts. Bakery products were developed with a partial replacement of wheat flour, using the centroid simplex method to understand the effect of applying NCFP flours in the final physical–chemical characteristics. The high granulometry associated with the high water absorption index indicates that the use of NCFP flours would have to be complemented with other flours for baking. The chemical composition of niger seed stood out the most; however, all seeds presented relatively low IC50 and MIC values for the inhibition of lipid peroxidation and antimicrobial activity, respectively. Finally, breads made with a mixture of millet and birdseed flour showed the greatest similarity to the control bread. Considering their composition in bioactive compounds, the use of these seeds is highly advisable in the context of a fortified diet, being sources of compounds of high nutritional value and with beneficial health effects for the final consumer.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry
IS  - 9
VL  - 11
DO  - 10.3390/agronomy11091873
SP  - 1873
ER  - 

@article{
author = "Lima, Juliana França and Dias, Maria Inês and Pereira, Carla and Ivanov, Marija and Soković, Marina and Steinmacher, Nádia Cristiane and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Consumers have given preferences to food products that, in addition to the nutritional properties, also present bioactive characteristics with beneficial health effects. The use of Nonconventional Food Plants (NCFP) has been an asset for the food industry, not only due to its abundance but, also, because it does not compete with other vegetable matrices used for human consumption for its nutritional properties, chemical and bioactive potentiality. The present work aimed to study the granulometry and water absorption index of three seeds of unconventional food plants: niger, millet and birdseed, followed by their nutritional value, content in free sugars, fatty acids, organic acids, tocopherols and phenolic compounds by chromatographic methods and, also, the evaluation of the antioxidant, hepatotoxic and antimicrobial potential in their hydroethanolic extracts. Bakery products were developed with a partial replacement of wheat flour, using the centroid simplex method to understand the effect of applying NCFP flours in the final physical–chemical characteristics. The high granulometry associated with the high water absorption index indicates that the use of NCFP flours would have to be complemented with other flours for baking. The chemical composition of niger seed stood out the most; however, all seeds presented relatively low IC50 and MIC values for the inhibition of lipid peroxidation and antimicrobial activity, respectively. Finally, breads made with a mixture of millet and birdseed flour showed the greatest similarity to the control bread. Considering their composition in bioactive compounds, the use of these seeds is highly advisable in the context of a fortified diet, being sources of compounds of high nutritional value and with beneficial health effects for the final consumer.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry",
number = "9",
volume = "11",
doi = "10.3390/agronomy11091873",
pages = "1873"
}

Lima, J. F., Dias, M. I., Pereira, C., Ivanov, M., Soković, M., Steinmacher, N. C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry. in Agronomy
Basel: MDPI., 11(9), 1873.
https://doi.org/10.3390/agronomy11091873

Lima JF, Dias MI, Pereira C, Ivanov M, Soković M, Steinmacher NC, Ferreira ICFR, Barros L. Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry. in Agronomy. 2021;11(9):1873.
doi:10.3390/agronomy11091873 .

Lima, Juliana França, Dias, Maria Inês, Pereira, Carla, Ivanov, Marija, Soković, Marina, Steinmacher, Nádia Cristiane, Ferreira, Isabel C. F. R., Barros, Lillian, "Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry" in Agronomy, 11, no. 9 (2021):1873,
https://doi.org/10.3390/agronomy11091873 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2073-4395/11/3/576
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4419
AB  - The Mediterranean basin is abundant in wild edible species with numerous health beneficial effects due to the presence of various bioactive phytochemicals. In the present work, the effect of nitrogen fertilization rates (0 ppm, (N0), 200 ppm (N1), 400 ppm (N2), and 600 ppm (N3) of total N) and growth substrate composition (soil or peat/perlite (2/1; v/v)) on the chemical composition and bioactive properties of Centaurea raphanina ssp. mixta plants was evaluated. The results of the study showed that both the tested factors affected nutritional value of the edible leaves, with the soil × N1 treatment being the most beneficial for fat, protein, and carbohydrate content and energetic value. On the other hand, the peat/perlite-grown plants that received 200 ppm of N had the highest content in α-, γ-, and total tocopherols, while the control treatment of soil-grown plants was the richest in individual and total sugars. Oxalic, citric, and total organic acids were the highest in the N2 × soil treatment, while malic acid was the highest in control treatment of the same substrate. The main fatty acids were palmitic, α-linolenic, and linoleic acids, with the highest contents being observed in the N0 × soil, N3 × soil, and N3 × peat/perlite treatments, respectively. The major phenolic compounds were pinocembrim neohesperidoside and pinocembrim acetyl neohesperidoside isomer II, with the highest content being observed in the N1 × soil treatment. The highest antihemolytic activity was observed in the N3 × peat/perlite treatment, while the most effective treatments against lipid peroxidation were N0 (in both soil and peat/perlite combinations) and N1 × peat/perlite. Lastly, all the tested extracts (except for N1 × soil) showed promising cytotoxic effects against HeLa (cervical carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), and NCI-H460 (non-small-cell lung cancer), while all the tested extracts exhibited better antifungal activities (lower minimal inhibition concentration (MIC) values) against Trichoderma viride than the positive controls. Overall, the present results suggest that the application of cost-effective practices such as the nitrogen application and the selection of growth substrate may regulate the chemical composition and the bioactive properties of C. raphanina ssp. mixta species and increase its added value under commercial cultivation conditions.
T2  - Agronomy
T1  - Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark
IS  - 3
VL  - 11
DO  - 10.3390/agronomy11030576
SP  - 576
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "The Mediterranean basin is abundant in wild edible species with numerous health beneficial effects due to the presence of various bioactive phytochemicals. In the present work, the effect of nitrogen fertilization rates (0 ppm, (N0), 200 ppm (N1), 400 ppm (N2), and 600 ppm (N3) of total N) and growth substrate composition (soil or peat/perlite (2/1; v/v)) on the chemical composition and bioactive properties of Centaurea raphanina ssp. mixta plants was evaluated. The results of the study showed that both the tested factors affected nutritional value of the edible leaves, with the soil × N1 treatment being the most beneficial for fat, protein, and carbohydrate content and energetic value. On the other hand, the peat/perlite-grown plants that received 200 ppm of N had the highest content in α-, γ-, and total tocopherols, while the control treatment of soil-grown plants was the richest in individual and total sugars. Oxalic, citric, and total organic acids were the highest in the N2 × soil treatment, while malic acid was the highest in control treatment of the same substrate. The main fatty acids were palmitic, α-linolenic, and linoleic acids, with the highest contents being observed in the N0 × soil, N3 × soil, and N3 × peat/perlite treatments, respectively. The major phenolic compounds were pinocembrim neohesperidoside and pinocembrim acetyl neohesperidoside isomer II, with the highest content being observed in the N1 × soil treatment. The highest antihemolytic activity was observed in the N3 × peat/perlite treatment, while the most effective treatments against lipid peroxidation were N0 (in both soil and peat/perlite combinations) and N1 × peat/perlite. Lastly, all the tested extracts (except for N1 × soil) showed promising cytotoxic effects against HeLa (cervical carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), and NCI-H460 (non-small-cell lung cancer), while all the tested extracts exhibited better antifungal activities (lower minimal inhibition concentration (MIC) values) against Trichoderma viride than the positive controls. Overall, the present results suggest that the application of cost-effective practices such as the nitrogen application and the selection of growth substrate may regulate the chemical composition and the bioactive properties of C. raphanina ssp. mixta species and increase its added value under commercial cultivation conditions.",
journal = "Agronomy",
title = "Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark",
number = "3",
volume = "11",
doi = "10.3390/agronomy11030576",
pages = "576"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark. in Agronomy, 11(3), 576.
https://doi.org/10.3390/agronomy11030576

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark. in Agronomy. 2021;11(3):576.
doi:10.3390/agronomy11030576 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark" in Agronomy, 11, no. 3 (2021):576,
https://doi.org/10.3390/agronomy11030576 . .

TY  - JOUR
AU  - Vega, Erika N.
AU  - Molina, Adriana K.
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Heleno, Sandrina A.
AU  - Rodrigues, Paula
AU  - Fernandes, Isabel P.
AU  - Barreiro, Maria Filomena
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Carocho, Marcio
AU  - Barreira, João C. M.
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4345
AB  - Given the importance of colour in the general acceptance or rejection of a product, the use of colorants is a widespread practice, particularly in the food industry. At the same time, with the increasing consumers’ awareness of the health effects that some artificial colorants can exert, there is a growing tendency to prioritize foodstuffs containing natural additives. In this work, Morus nigra L. and Rubus fruticosus L. fruit juices were characterized in terms of anthocyanins, organic acids, free sugars, and tocopherols, as also regarding their bioactive properties. Given their richness in anthocyanins, this study also aimed to prepare different solid colouring formulations by the spray-drying technique, using as stabilizers maltodextrin and arabic gum. Six free sugars and two organic acids were detected in the fruit juices, as well as the four tocopherol isoforms. Two cyanidin derivatives were found in M. nigra (cyanidin-3-O-glucoside and cyanidin-O-rhamnoside) and other four in R. fruticosus (cyanidin-O-hexoside, cyanidin-3-O-glucoside, cyanidin-O-pentoside, and cyanidin-3-O-dioxaloilglucoside). The developed colouring formulations revealed a good stability over time, in terms of anthocyanin concentration and colour parameters, and revealed to be safe for consumption, either concerning their low microbial load and lack of cytotoxicity. Thus, they represent a promising natural alternative to the massively used artificial colorants.
PB  - Basel: MDPI
T2  - Plants
T1  - Anthocyanins from rubus fruticosus l. And morus nigra l. applied as food colorants: A natural alternative
IS  - 6
VL  - 10
DO  - 10.3390/plants10061181
SP  - 1181
ER  - 

@article{
author = "Vega, Erika N. and Molina, Adriana K. and Pereira, Carla and Dias, Maria Inês and Heleno, Sandrina A. and Rodrigues, Paula and Fernandes, Isabel P. and Barreiro, Maria Filomena and Stojković, Dejan and Soković, Marina and Carocho, Marcio and Barreira, João C. M. and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Given the importance of colour in the general acceptance or rejection of a product, the use of colorants is a widespread practice, particularly in the food industry. At the same time, with the increasing consumers’ awareness of the health effects that some artificial colorants can exert, there is a growing tendency to prioritize foodstuffs containing natural additives. In this work, Morus nigra L. and Rubus fruticosus L. fruit juices were characterized in terms of anthocyanins, organic acids, free sugars, and tocopherols, as also regarding their bioactive properties. Given their richness in anthocyanins, this study also aimed to prepare different solid colouring formulations by the spray-drying technique, using as stabilizers maltodextrin and arabic gum. Six free sugars and two organic acids were detected in the fruit juices, as well as the four tocopherol isoforms. Two cyanidin derivatives were found in M. nigra (cyanidin-3-O-glucoside and cyanidin-O-rhamnoside) and other four in R. fruticosus (cyanidin-O-hexoside, cyanidin-3-O-glucoside, cyanidin-O-pentoside, and cyanidin-3-O-dioxaloilglucoside). The developed colouring formulations revealed a good stability over time, in terms of anthocyanin concentration and colour parameters, and revealed to be safe for consumption, either concerning their low microbial load and lack of cytotoxicity. Thus, they represent a promising natural alternative to the massively used artificial colorants.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Anthocyanins from rubus fruticosus l. And morus nigra l. applied as food colorants: A natural alternative",
number = "6",
volume = "10",
doi = "10.3390/plants10061181",
pages = "1181"
}

Vega, E. N., Molina, A. K., Pereira, C., Dias, M. I., Heleno, S. A., Rodrigues, P., Fernandes, I. P., Barreiro, M. F., Stojković, D., Soković, M., Carocho, M., Barreira, J. C. M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Anthocyanins from rubus fruticosus l. And morus nigra l. applied as food colorants: A natural alternative. in Plants
Basel: MDPI., 10(6), 1181.
https://doi.org/10.3390/plants10061181

Vega EN, Molina AK, Pereira C, Dias MI, Heleno SA, Rodrigues P, Fernandes IP, Barreiro MF, Stojković D, Soković M, Carocho M, Barreira JCM, Ferreira ICFR, Barros L. Anthocyanins from rubus fruticosus l. And morus nigra l. applied as food colorants: A natural alternative. in Plants. 2021;10(6):1181.
doi:10.3390/plants10061181 .

Vega, Erika N., Molina, Adriana K., Pereira, Carla, Dias, Maria Inês, Heleno, Sandrina A., Rodrigues, Paula, Fernandes, Isabel P., Barreiro, Maria Filomena, Stojković, Dejan, Soković, Marina, Carocho, Marcio, Barreira, João C. M., Ferreira, Isabel C. F. R., Barros, Lillian, "Anthocyanins from rubus fruticosus l. And morus nigra l. applied as food colorants: A natural alternative" in Plants, 10, no. 6 (2021):1181,
https://doi.org/10.3390/plants10061181 . .

TY  - JOUR
AU  - Pires, Eleomar de O.
AU  - Pereira, Eliana
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo C.
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Hassemer, Gustavo
AU  - Garcia, Carolina Castilho
AU  - Caleja, Cristina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2021
UR  - https://www.mdpi.com/1420-3049/26/5/1347
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4210
AB  - The attractive colour characteristics of the flowers of the species Impatiens walleriana have been arousing great interest in the food industry, which is looking for potential natural sources of colouring ingredients. In this sense, the present work focused on the chemical and bioactive characterization of pink and orange flowers of I. walleriana. The phenolic compounds were determined by HPLC-DAD-ESI/MS; in addition, different bioactivities (antioxidant, antimicrobial, anti-inflammatory and cytotoxicity) were also analysed. Both samples studied showed significant amounts of phenolic compounds, especially phenolic acids, flavonoids, and anthocyanins, which justifies the excellent performance in the different bioactivities studied. The orange variety, despite having a greater variety of phenolic compounds, showed a total amount of compounds lower than the pink variety. Overall, the flowers of I. walleriana emerge as a promising resource to be explored by the food industry.
PB  - MDPI
T2  - Molecules
T1  - Chemical Composition and Bioactive Characterisation of Impatiens walleriana
IS  - 5
VL  - 26
DO  - 10.3390/molecules26051347
SP  - 1347
ER  - 

@article{
author = "Pires, Eleomar de O. and Pereira, Eliana and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo C. and Ćirić, Ana and Soković, Marina and Hassemer, Gustavo and Garcia, Carolina Castilho and Caleja, Cristina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2021",
abstract = "The attractive colour characteristics of the flowers of the species Impatiens walleriana have been arousing great interest in the food industry, which is looking for potential natural sources of colouring ingredients. In this sense, the present work focused on the chemical and bioactive characterization of pink and orange flowers of I. walleriana. The phenolic compounds were determined by HPLC-DAD-ESI/MS; in addition, different bioactivities (antioxidant, antimicrobial, anti-inflammatory and cytotoxicity) were also analysed. Both samples studied showed significant amounts of phenolic compounds, especially phenolic acids, flavonoids, and anthocyanins, which justifies the excellent performance in the different bioactivities studied. The orange variety, despite having a greater variety of phenolic compounds, showed a total amount of compounds lower than the pink variety. Overall, the flowers of I. walleriana emerge as a promising resource to be explored by the food industry.",
publisher = "MDPI",
journal = "Molecules",
title = "Chemical Composition and Bioactive Characterisation of Impatiens walleriana",
number = "5",
volume = "26",
doi = "10.3390/molecules26051347",
pages = "1347"
}

Pires, E. d. O., Pereira, E., Pereira, C., Dias, M. I., Calhelha, R. C., Ćirić, A., Soković, M., Hassemer, G., Garcia, C. C., Caleja, C., Barros, L.,& Ferreira, I. C. F. R.. (2021). Chemical Composition and Bioactive Characterisation of Impatiens walleriana. in Molecules
MDPI., 26(5), 1347.
https://doi.org/10.3390/molecules26051347

Pires EDO, Pereira E, Pereira C, Dias MI, Calhelha RC, Ćirić A, Soković M, Hassemer G, Garcia CC, Caleja C, Barros L, Ferreira ICFR. Chemical Composition and Bioactive Characterisation of Impatiens walleriana. in Molecules. 2021;26(5):1347.
doi:10.3390/molecules26051347 .

Pires, Eleomar de O., Pereira, Eliana, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo C., Ćirić, Ana, Soković, Marina, Hassemer, Gustavo, Garcia, Carolina Castilho, Caleja, Cristina, Barros, Lillian, Ferreira, Isabel C. F. R., "Chemical Composition and Bioactive Characterisation of Impatiens walleriana" in Molecules, 26, no. 5 (2021):1347,
https://doi.org/10.3390/molecules26051347 . .

TY  - JOUR
AU  - Silva, Gabriel F. Pantuzza
AU  - Pereira, Eliana
AU  - Melgar, Bruno
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Pereira, Carla
AU  - Abreu, Rui M. V.
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2076-3417/11/1/151
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4094
AB  - Consumers are very concerned with following a healthy diet, along with some precautions that may influence environmental impact. Solanum melongena L. is one of the most consumed vegetables due to its excellent nutritional value and antioxidant action. Associated with its high consumption, considerable amounts of agro-food wastes are produced. This work targets the valorization of this matrix, through the use of its bio-residues to study the obtention of coloring pigments, applying innovative technologies. Its nutritional value, chemical composition, and bioactive potential were evaluated, and the ultrasound-assisted extraction to obtain coloring pigments of high industrial interest was optimized. Considering the results, low contents of fat and carbohydrates and energy value were evident, as well as the presence of compounds of interest (free sugars, organic acids, unsaturated fatty acids, and phenolic acids). In addition, the antioxidant and antimicrobial potential was detected. Response surface methodology was performed to optimize the extraction of natural pigments, showing a concentration of 11.9 mg/g of anthocyanins/g of extract, applying optimal conditions of time, solvent, and solid/liquid ratio of 0.5 min, 68.2% (v/v) and 5 g/L, respectively. S. melongena proved to be a good source of bioactive compounds and natural pigments, which can generate great interest in the food industry.
PB  - MDPI AG
T2  - Applied Sciences
T1  - Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies
IS  - 1
VL  - 11
DO  - 10.3390/app11010151
SP  - 151
ER  - 

@article{
author = "Silva, Gabriel F. Pantuzza and Pereira, Eliana and Melgar, Bruno and Stojković, Dejan and Soković, Marina and Calhelha, Ricardo C. and Pereira, Carla and Abreu, Rui M. V. and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Consumers are very concerned with following a healthy diet, along with some precautions that may influence environmental impact. Solanum melongena L. is one of the most consumed vegetables due to its excellent nutritional value and antioxidant action. Associated with its high consumption, considerable amounts of agro-food wastes are produced. This work targets the valorization of this matrix, through the use of its bio-residues to study the obtention of coloring pigments, applying innovative technologies. Its nutritional value, chemical composition, and bioactive potential were evaluated, and the ultrasound-assisted extraction to obtain coloring pigments of high industrial interest was optimized. Considering the results, low contents of fat and carbohydrates and energy value were evident, as well as the presence of compounds of interest (free sugars, organic acids, unsaturated fatty acids, and phenolic acids). In addition, the antioxidant and antimicrobial potential was detected. Response surface methodology was performed to optimize the extraction of natural pigments, showing a concentration of 11.9 mg/g of anthocyanins/g of extract, applying optimal conditions of time, solvent, and solid/liquid ratio of 0.5 min, 68.2% (v/v) and 5 g/L, respectively. S. melongena proved to be a good source of bioactive compounds and natural pigments, which can generate great interest in the food industry.",
publisher = "MDPI AG",
journal = "Applied Sciences",
title = "Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies",
number = "1",
volume = "11",
doi = "10.3390/app11010151",
pages = "151"
}

Silva, G. F. P., Pereira, E., Melgar, B., Stojković, D., Soković, M., Calhelha, R. C., Pereira, C., Abreu, R. M. V., Ferreira, I. C. F. R.,& Barros, L.. (2021). Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies. in Applied Sciences
MDPI AG., 11(1), 151.
https://doi.org/10.3390/app11010151

Silva GFP, Pereira E, Melgar B, Stojković D, Soković M, Calhelha RC, Pereira C, Abreu RMV, Ferreira ICFR, Barros L. Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies. in Applied Sciences. 2021;11(1):151.
doi:10.3390/app11010151 .

Silva, Gabriel F. Pantuzza, Pereira, Eliana, Melgar, Bruno, Stojković, Dejan, Soković, Marina, Calhelha, Ricardo C., Pereira, Carla, Abreu, Rui M. V., Ferreira, Isabel C. F. R., Barros, Lillian, "Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies" in Applied Sciences, 11, no. 1 (2021):151,
https://doi.org/10.3390/app11010151 . .

TY  - JOUR
AU  - Ben Ayache, Siwar
AU  - Reis, Filipa S.
AU  - Inês Dias, Maria
AU  - Pereira, Carla
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Behija Saafi, Emna
AU  - C. F. R. Ferreira, Isabel
AU  - Barros, Lillian
AU  - Achour, Lotfi
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814621002673
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4157
AB  - Nowadays, the use of carob (Ceratonia siliqua L.) is reduced to its seeds. In fact, the food additive E410, locust bean gum, is obtained from its endosperm. However, the available information regarding the bioactive potential of carob seeds is limited and, often, with poorly established terminologies, not allowing the reader to know if the studied samples included seedless or seeded samples. So, the present study intended to carry out a chemical characterization of carob seeds focused on their bioactive compounds and test their bioactive properties. Carob seeds proved to be a source of tocopherols and organic acids, including phenolic compounds. Its antioxidant potential was demonstrated in vitro, as well as its antimicrobial capacity. This work proves that carob seeds have other functions in addition to those associated with E410. Thus, another potential can be given to the locust bean seed, namely the function of food preservative.
PB  - Elsevier BV
T2  - Food Chemistry
T1  - Chemical characterization of carob seeds (Ceratonia siliqua L.) and use of different extraction techniques to promote its bioactivity
VL  - 351
DO  - 10.1016/j.foodchem.2021.129263
SP  - 129263
ER  - 

@article{
author = "Ben Ayache, Siwar and Reis, Filipa S. and Inês Dias, Maria and Pereira, Carla and Glamočlija, Jasmina and Soković, Marina and Behija Saafi, Emna and C. F. R. Ferreira, Isabel and Barros, Lillian and Achour, Lotfi",
year = "2021",
abstract = "Nowadays, the use of carob (Ceratonia siliqua L.) is reduced to its seeds. In fact, the food additive E410, locust bean gum, is obtained from its endosperm. However, the available information regarding the bioactive potential of carob seeds is limited and, often, with poorly established terminologies, not allowing the reader to know if the studied samples included seedless or seeded samples. So, the present study intended to carry out a chemical characterization of carob seeds focused on their bioactive compounds and test their bioactive properties. Carob seeds proved to be a source of tocopherols and organic acids, including phenolic compounds. Its antioxidant potential was demonstrated in vitro, as well as its antimicrobial capacity. This work proves that carob seeds have other functions in addition to those associated with E410. Thus, another potential can be given to the locust bean seed, namely the function of food preservative.",
publisher = "Elsevier BV",
journal = "Food Chemistry",
title = "Chemical characterization of carob seeds (Ceratonia siliqua L.) and use of different extraction techniques to promote its bioactivity",
volume = "351",
doi = "10.1016/j.foodchem.2021.129263",
pages = "129263"
}

Ben Ayache, S., Reis, F. S., Inês Dias, M., Pereira, C., Glamočlija, J., Soković, M., Behija Saafi, E., C. F. R. Ferreira, I., Barros, L.,& Achour, L.. (2021). Chemical characterization of carob seeds (Ceratonia siliqua L.) and use of different extraction techniques to promote its bioactivity. in Food Chemistry
Elsevier BV., 351, 129263.
https://doi.org/10.1016/j.foodchem.2021.129263

Ben Ayache S, Reis FS, Inês Dias M, Pereira C, Glamočlija J, Soković M, Behija Saafi E, C. F. R. Ferreira I, Barros L, Achour L. Chemical characterization of carob seeds (Ceratonia siliqua L.) and use of different extraction techniques to promote its bioactivity. in Food Chemistry. 2021;351:129263.
doi:10.1016/j.foodchem.2021.129263 .

Ben Ayache, Siwar, Reis, Filipa S., Inês Dias, Maria, Pereira, Carla, Glamočlija, Jasmina, Soković, Marina, Behija Saafi, Emna, C. F. R. Ferreira, Isabel, Barros, Lillian, Achour, Lotfi, "Chemical characterization of carob seeds (Ceratonia siliqua L.) and use of different extraction techniques to promote its bioactivity" in Food Chemistry, 351 (2021):129263,
https://doi.org/10.1016/j.foodchem.2021.129263 . .

TY  - JOUR
AU  - García-Pérez, Pascual
AU  - Ayuso, Manuel
AU  - Lozano-Milo, Eva
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Ivanov, Marija
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
AU  - Gallego, Pedro P.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4207
AB  - Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants
VL  - 162
DO  - 10.1016/j.indcrop.2021.113241
SP  - 113241
ER  - 

@article{
author = "García-Pérez, Pascual and Ayuso, Manuel and Lozano-Milo, Eva and Pereira, Carla and Dias, Maria Inês and Ivanov, Marija and Calhelha, Ricardo C. and Soković, Marina and Ferreira, Isabel C.F.R. and Barros, Lillian and Gallego, Pedro P.",
year = "2021",
abstract = "Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants",
volume = "162",
doi = "10.1016/j.indcrop.2021.113241",
pages = "113241"
}

García-Pérez, P., Ayuso, M., Lozano-Milo, E., Pereira, C., Dias, M. I., Ivanov, M., Calhelha, R. C., Soković, M., Ferreira, I. C.F.R., Barros, L.,& Gallego, P. P.. (2021). Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products
Elsevier B.V.., 162, 113241.
https://doi.org/10.1016/j.indcrop.2021.113241

García-Pérez P, Ayuso M, Lozano-Milo E, Pereira C, Dias MI, Ivanov M, Calhelha RC, Soković M, Ferreira IC, Barros L, Gallego PP. Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products. 2021;162:113241.
doi:10.1016/j.indcrop.2021.113241 .

García-Pérez, Pascual, Ayuso, Manuel, Lozano-Milo, Eva, Pereira, Carla, Dias, Maria Inês, Ivanov, Marija, Calhelha, Ricardo C., Soković, Marina, Ferreira, Isabel C.F.R., Barros, Lillian, Gallego, Pedro P., "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants" in Industrial Crops and Products, 162 (2021):113241,
https://doi.org/10.1016/j.indcrop.2021.113241 . .

TY  - JOUR
AU  - Albuquerque, Bianca R.
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Oliveira, M. Beatriz P. P.
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2304-8158/10/4/700
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4218
AB  - Fruit bio-residues can be interesting for the recovery of bioactive molecules, such as phenolic compounds, tocopherols, vitamins, among others. These compounds can be targeted at the food industry and used for the development of functional foods or as food additives. In some cases, fruit epicarps are converted into by-products with non-commercial value, and generally, these fruit parts have a higher content in bioactive compounds than the fruit pulp. From this perspective, S. odorifera, a Brazilian fruit, has an inedible epicarp that could be explored to obtain biological compounds. Therefore, the aims of this study were to evaluate the chemical composition and the antioxidant, anti-proliferative, anti-inflammatory, and antimicrobial bioactivities of this by-product. S. odorifera epicarp showed a total of four organic acids, four phenolic compounds, highlighting the high concentration of anthocyanins (24 ± 1 mg/g dry weight (dw)) and high content of tocopherols (366 ± 2 mg/100 g dw). The hydroethanolic extract showed considerable antioxidant activity (EC50 values of 48.2 ± 0.5 and 27 ± 1 µg/mL for TBARS and OxHLIA assays, respectively), as also antibacterial and antifungal activities (minimal inhibitory concentrations (MICs) ≤ 2.2 mg/mL). The results obtained in this study suggest that Sicana odorifera epicarp represents a reliable option for the development of novel natural-based colorants with functional/bioactive proprieties.
PB  - MDPI AG
T2  - Foods
T1  - Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties
IS  - 4
VL  - 10
DO  - 10.3390/foods10040700
SP  - 700
ER  - 

@article{
author = "Albuquerque, Bianca R. and Dias, Maria Inês and Pereira, Carla and Petrović, Jovana and Soković, Marina and Calhelha, Ricardo C. and Oliveira, M. Beatriz P. P. and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Fruit bio-residues can be interesting for the recovery of bioactive molecules, such as phenolic compounds, tocopherols, vitamins, among others. These compounds can be targeted at the food industry and used for the development of functional foods or as food additives. In some cases, fruit epicarps are converted into by-products with non-commercial value, and generally, these fruit parts have a higher content in bioactive compounds than the fruit pulp. From this perspective, S. odorifera, a Brazilian fruit, has an inedible epicarp that could be explored to obtain biological compounds. Therefore, the aims of this study were to evaluate the chemical composition and the antioxidant, anti-proliferative, anti-inflammatory, and antimicrobial bioactivities of this by-product. S. odorifera epicarp showed a total of four organic acids, four phenolic compounds, highlighting the high concentration of anthocyanins (24 ± 1 mg/g dry weight (dw)) and high content of tocopherols (366 ± 2 mg/100 g dw). The hydroethanolic extract showed considerable antioxidant activity (EC50 values of 48.2 ± 0.5 and 27 ± 1 µg/mL for TBARS and OxHLIA assays, respectively), as also antibacterial and antifungal activities (minimal inhibitory concentrations (MICs) ≤ 2.2 mg/mL). The results obtained in this study suggest that Sicana odorifera epicarp represents a reliable option for the development of novel natural-based colorants with functional/bioactive proprieties.",
publisher = "MDPI AG",
journal = "Foods",
title = "Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties",
number = "4",
volume = "10",
doi = "10.3390/foods10040700",
pages = "700"
}

Albuquerque, B. R., Dias, M. I., Pereira, C., Petrović, J., Soković, M., Calhelha, R. C., Oliveira, M. B. P. P., Ferreira, I. C. F. R.,& Barros, L.. (2021). Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties. in Foods
MDPI AG., 10(4), 700.
https://doi.org/10.3390/foods10040700

Albuquerque BR, Dias MI, Pereira C, Petrović J, Soković M, Calhelha RC, Oliveira MBPP, Ferreira ICFR, Barros L. Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties. in Foods. 2021;10(4):700.
doi:10.3390/foods10040700 .

Albuquerque, Bianca R., Dias, Maria Inês, Pereira, Carla, Petrović, Jovana, Soković, Marina, Calhelha, Ricardo C., Oliveira, M. Beatriz P. P., Ferreira, Isabel C. F. R., Barros, Lillian, "Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties" in Foods, 10, no. 4 (2021):700,
https://doi.org/10.3390/foods10040700 . .

TY  - JOUR
AU  - Carpena, Marija
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Pereira, Carla
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Soria-Lopez, Anton
AU  - Fraga-Corral, Maria
AU  - Simal-Gandara, Jesus
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Prieto, Miguel A.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4359
AB  - The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.
PB  - Basel: MDPI
T2  - Chemosensors
T1  - Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction
IS  - 6
VL  - 9
DO  - 10.3390/chemosensors9060132
SP  - 132
ER  - 

@article{
author = "Carpena, Marija and Caleja, Cristina and Pereira, Eliana and Pereira, Carla and Ćirić, Ana and Soković, Marina and Soria-Lopez, Anton and Fraga-Corral, Maria and Simal-Gandara, Jesus and Ferreira, Isabel C. F. R. and Barros, Lillian and Prieto, Miguel A.",
year = "2021",
abstract = "The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.",
publisher = "Basel: MDPI",
journal = "Chemosensors",
title = "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction",
number = "6",
volume = "9",
doi = "10.3390/chemosensors9060132",
pages = "132"
}

Carpena, M., Caleja, C., Pereira, E., Pereira, C., Ćirić, A., Soković, M., Soria-Lopez, A., Fraga-Corral, M., Simal-Gandara, J., Ferreira, I. C. F. R., Barros, L.,& Prieto, M. A.. (2021). Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors
Basel: MDPI., 9(6), 132.
https://doi.org/10.3390/chemosensors9060132

Carpena M, Caleja C, Pereira E, Pereira C, Ćirić A, Soković M, Soria-Lopez A, Fraga-Corral M, Simal-Gandara J, Ferreira ICFR, Barros L, Prieto MA. Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors. 2021;9(6):132.
doi:10.3390/chemosensors9060132 .

Carpena, Marija, Caleja, Cristina, Pereira, Eliana, Pereira, Carla, Ćirić, Ana, Soković, Marina, Soria-Lopez, Anton, Fraga-Corral, Maria, Simal-Gandara, Jesus, Ferreira, Isabel C. F. R., Barros, Lillian, Prieto, Miguel A., "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction" in Chemosensors, 9, no. 6 (2021):132,
https://doi.org/10.3390/chemosensors9060132 . .

TY  - JOUR
AU  - Pires Jr, Eleomar de O
AU  - Pereira, Eliana
AU  - Carocho, Márcio
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Garcia, Carolina C.
AU  - Ferreira, Isabel C. F. R.
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4382
AB  - Flowers of the genus Impatiens are classified as edible; however, their inclusion in the human
diet is not yet a common practice. Its attractive colours have stirred great interest by the food industry.
In this sense, rose (BP) and orange (BO) I. balsamina flowers were nutritionally studied, followed by
an in-depth chemical study profile. The non-anthocyanin and anthocyanin profiles of extracts of
both flower varieties were also determined by high-performance liquid chromatography coupled
to a diode array and mass spectrometry detector (HPLC-DAD-ESI/MS). The results demonstrated
that both varieties presented significant amounts of phenolic compounds, having identified nine
non-anthocyanin compounds and 14 anthocyanin compounds. BP extract stood out in its bioactive
properties (antioxidant and antimicrobial potential) and was selected for incorporation in “bombocas”
filling. Its performance as a colouring ingredient was compared with the control formulations (white
filling) and with E163 (anthocyanins) colorant. The incorporation of the natural ingredient did not
cause changes in the chemical and nutritional composition of the product; and although the colour
conferred was lighter than presented by the formulation with E163 (suggesting a more natural aspect),
the higher antioxidant activity could meet the expectations of the current high-demand consumer.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product
IS  - 17
VL  - 18
DO  - 10.3390/ijerph18179062
SP  - 9062
ER  - 

@article{
author = "Pires Jr, Eleomar de O and Pereira, Eliana and Carocho, Márcio and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo and Ćirić, Ana and Soković, Marina and Garcia, Carolina C. and Ferreira, Isabel C. F. R. and Caleja, Cristina and Barros, Lillian",
year = "2021",
abstract = "Flowers of the genus Impatiens are classified as edible; however, their inclusion in the human
diet is not yet a common practice. Its attractive colours have stirred great interest by the food industry.
In this sense, rose (BP) and orange (BO) I. balsamina flowers were nutritionally studied, followed by
an in-depth chemical study profile. The non-anthocyanin and anthocyanin profiles of extracts of
both flower varieties were also determined by high-performance liquid chromatography coupled
to a diode array and mass spectrometry detector (HPLC-DAD-ESI/MS). The results demonstrated
that both varieties presented significant amounts of phenolic compounds, having identified nine
non-anthocyanin compounds and 14 anthocyanin compounds. BP extract stood out in its bioactive
properties (antioxidant and antimicrobial potential) and was selected for incorporation in “bombocas”
filling. Its performance as a colouring ingredient was compared with the control formulations (white
filling) and with E163 (anthocyanins) colorant. The incorporation of the natural ingredient did not
cause changes in the chemical and nutritional composition of the product; and although the colour
conferred was lighter than presented by the formulation with E163 (suggesting a more natural aspect),
the higher antioxidant activity could meet the expectations of the current high-demand consumer.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product",
number = "17",
volume = "18",
doi = "10.3390/ijerph18179062",
pages = "9062"
}

Pires Jr, E. d. O., Pereira, E., Carocho, M., Pereira, C., Dias, M. I., Calhelha, R., Ćirić, A., Soković, M., Garcia, C. C., Ferreira, I. C. F. R., Caleja, C.,& Barros, L.. (2021). Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(17), 9062.
https://doi.org/10.3390/ijerph18179062

Pires Jr EDO, Pereira E, Carocho M, Pereira C, Dias MI, Calhelha R, Ćirić A, Soković M, Garcia CC, Ferreira ICFR, Caleja C, Barros L. Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product. in International Journal of Environmental Research and Public Health. 2021;18(17):9062.
doi:10.3390/ijerph18179062 .

Pires Jr, Eleomar de O, Pereira, Eliana, Carocho, Márcio, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo, Ćirić, Ana, Soković, Marina, Garcia, Carolina C., Ferreira, Isabel C. F. R., Caleja, Cristina, Barros, Lillian, "Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product" in International Journal of Environmental Research and Public Health, 18, no. 17 (2021):9062,
https://doi.org/10.3390/ijerph18179062 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Plexida, Sofia
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2071-1050/12/20/8511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3943
AB  - The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.
PB  - MDPI AG
T2  - Sustainability
T1  - The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production
IS  - 20
VL  - 12
DO  - 10.3390/su12208511
SP  - 8511
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Plexida, Sofia and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Petrović, Jovana and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.",
publisher = "MDPI AG",
journal = "Sustainability",
title = "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production",
number = "20",
volume = "12",
doi = "10.3390/su12208511",
pages = "8511"
}

Petropoulos, S. A., Fernandes, Â., Plexida, S., Pereira, C., Dias, M. I., Calhelha, R., Chrysargyris, A., Tzortzakis, N., Petrović, J., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability
MDPI AG., 12(20), 8511.
https://doi.org/10.3390/su12208511

Petropoulos SA, Fernandes Â, Plexida S, Pereira C, Dias MI, Calhelha R, Chrysargyris A, Tzortzakis N, Petrović J, Soković M, Ferreira ICFR, Barros L. The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability. 2020;12(20):8511.
doi:10.3390/su12208511 .

Petropoulos, Spyridon A., Fernandes, Ângela, Plexida, Sofia, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo, Chrysargyris, Antonios, Tzortzakis, Nikolaos, Petrović, Jovana, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production" in Sustainability, 12, no. 20 (2020):8511,
https://doi.org/10.3390/su12208511 . .

TY  - JOUR
AU  - Ghada, Bejaoui
AU  - Pereira, Eliana
AU  - Pinela, José
AU  - Prieto, Miguel A.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Zaghdoudi, Khalil
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3203
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32674320
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3830
AB  - The potential of passion fruit (Passiflora edulis Sims) epicarp to produce anthocyanin-based colorants with bioactive properties was evaluated. First, a five-level three-factor factorial design coupled with response surface methodology was implemented to optimize the extraction of anthocyanins from dark purple epicarps. The extraction yield and cyanidin-3-O-glucoside content were used as response criteria. The constructed models were fitted to the experimental data and used to calculate the optimal processing conditions (t = 38 min, T = 20 °C, S = 0% ethanol/water (v/v) acidified with citric acid to pH 3, and RS/L = 50 g/L) that lead to maximum responses (3.4 mg/g dried epicarp and 9 mg/g extract). Then, the antioxidant, antimicrobial, and cytotoxic activities of anthocyanin extracts obtained using the optimized method and a conventional extraction method were evaluated in vitro. The extract obtained by the optimized method revealed a higher bioactivity, in agreement with the higher cyanidin-3-O-glucoside content. This study highlighted the coloring and bioactive potential of a bio-based ingredient recycled from a bio-waste, which promotes a sustainable bioeconomy in the agri-food sector.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143203
SP  - 3203
ER  - 

@article{
author = "Ghada, Bejaoui and Pereira, Eliana and Pinela, José and Prieto, Miguel A. and Pereira, Carla and Calhelha, Ricardo C. and Stojković, Dejan and Soković, Marina and Zaghdoudi, Khalil and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "The potential of passion fruit (Passiflora edulis Sims) epicarp to produce anthocyanin-based colorants with bioactive properties was evaluated. First, a five-level three-factor factorial design coupled with response surface methodology was implemented to optimize the extraction of anthocyanins from dark purple epicarps. The extraction yield and cyanidin-3-O-glucoside content were used as response criteria. The constructed models were fitted to the experimental data and used to calculate the optimal processing conditions (t = 38 min, T = 20 °C, S = 0% ethanol/water (v/v) acidified with citric acid to pH 3, and RS/L = 50 g/L) that lead to maximum responses (3.4 mg/g dried epicarp and 9 mg/g extract). Then, the antioxidant, antimicrobial, and cytotoxic activities of anthocyanin extracts obtained using the optimized method and a conventional extraction method were evaluated in vitro. The extract obtained by the optimized method revealed a higher bioactivity, in agreement with the higher cyanidin-3-O-glucoside content. This study highlighted the coloring and bioactive potential of a bio-based ingredient recycled from a bio-waste, which promotes a sustainable bioeconomy in the agri-food sector.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143203",
pages = "3203"
}

Ghada, B., Pereira, E., Pinela, J., Prieto, M. A., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Zaghdoudi, K., Barros, L.,& Ferreira, I. C. F. R.. (2020). Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3203.
https://doi.org/10.3390/molecules25143203

Ghada B, Pereira E, Pinela J, Prieto MA, Pereira C, Calhelha RC, Stojković D, Soković M, Zaghdoudi K, Barros L, Ferreira ICFR. Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.. in Molecules (Basel, Switzerland). 2020;25(14):3203.
doi:10.3390/molecules25143203 .

Ghada, Bejaoui, Pereira, Eliana, Pinela, José, Prieto, Miguel A., Pereira, Carla, Calhelha, Ricardo C., Stojković, Dejan, Soković, Marina, Zaghdoudi, Khalil, Barros, Lillian, Ferreira, Isabel C. F. R., "Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3203,
https://doi.org/10.3390/molecules25143203 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3175
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32664565
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3829
AB  - The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143175
SP  - 3175
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C F R and Barros, Lillian",
year = "2020",
abstract = "The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143175",
pages = "3175"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3175.
https://doi.org/10.3390/molecules25143175

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland). 2020;25(14):3175.
doi:10.3390/molecules25143175 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C F R, Barros, Lillian, "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3175,
https://doi.org/10.3390/molecules25143175 . .

TY  - JOUR
AU  - Gonzales-Barron, Ursula
AU  - Dijkshoorn, Rody
AU  - Maloncy, Maikel
AU  - Finimundy, Tiane
AU  - Calhelha, Ricardo C.
AU  - Pereira, Carla
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Cadavez, Vasco
PY  - 2020
UR  - https://www.mdpi.com/2304-8158/9/5/597
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7278699
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3692
AB  - Although the nutritional profile, bioactivities, and uses of mesquite pod flour from various Prosopis species have been studied, limited research has been conducted on Prosopis pallida (Humb, & Bonpl. Ex Willd.) Kunth mesquite flour. This study aimed to characterize the nutritional quality and bioactive properties of P. pallida pod flour and to assess its technological performance in breadmaking as a partial replacer of white wheat flour. Peruvian P. pallida mesquite flour was found to have an appealing nutritional profile, with high contents of dietary fiber (29.6% dw) and protein (9.5% dw), and low contents of fat (1.0% dw) and carbohydrates (57.6% dw). It is a source of palmitic (12.6%), oleic (35.5%), and linoleic acids (45.8%), α-, β-, and γ- tocopherols, and contains phenolic compounds such as apigenin glycoside derivatives with proven antioxidant capacities. Extracts of P. pallida flour were also found to have antimicrobial and antifungal effects and did not show hepatoxicity. When formulated as a wheat flour replacer, increasing mesquite flour levels yield composite doughs of lower stickiness and extensibility, and composite breads of lower elasticity (p < 0.01). However, up to a level of 10%, mesquite flour significantly increases loaf volume, reduces crumb hardness, and produces a more uniform crumb of small size alveoli (p < 0.01). Considering the purpose of improving the nutritional and technological quality of wheat flour bread, the addition of P. pallida pod flour can be highly recommended.
PB  - MDPI Multidisciplinary Digital Publishing Institute
T2  - Foods (Basel, Switzerland)
T1  - Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.
IS  - 5
VL  - 9
DO  - 10.3390/foods9050597
SP  - 597
ER  - 

@article{
author = "Gonzales-Barron, Ursula and Dijkshoorn, Rody and Maloncy, Maikel and Finimundy, Tiane and Calhelha, Ricardo C. and Pereira, Carla and Stojković, Dejan and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian and Cadavez, Vasco",
year = "2020",
abstract = "Although the nutritional profile, bioactivities, and uses of mesquite pod flour from various Prosopis species have been studied, limited research has been conducted on Prosopis pallida (Humb, & Bonpl. Ex Willd.) Kunth mesquite flour. This study aimed to characterize the nutritional quality and bioactive properties of P. pallida pod flour and to assess its technological performance in breadmaking as a partial replacer of white wheat flour. Peruvian P. pallida mesquite flour was found to have an appealing nutritional profile, with high contents of dietary fiber (29.6% dw) and protein (9.5% dw), and low contents of fat (1.0% dw) and carbohydrates (57.6% dw). It is a source of palmitic (12.6%), oleic (35.5%), and linoleic acids (45.8%), α-, β-, and γ- tocopherols, and contains phenolic compounds such as apigenin glycoside derivatives with proven antioxidant capacities. Extracts of P. pallida flour were also found to have antimicrobial and antifungal effects and did not show hepatoxicity. When formulated as a wheat flour replacer, increasing mesquite flour levels yield composite doughs of lower stickiness and extensibility, and composite breads of lower elasticity (p < 0.01). However, up to a level of 10%, mesquite flour significantly increases loaf volume, reduces crumb hardness, and produces a more uniform crumb of small size alveoli (p < 0.01). Considering the purpose of improving the nutritional and technological quality of wheat flour bread, the addition of P. pallida pod flour can be highly recommended.",
publisher = "MDPI Multidisciplinary Digital Publishing Institute",
journal = "Foods (Basel, Switzerland)",
title = "Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.",
number = "5",
volume = "9",
doi = "10.3390/foods9050597",
pages = "597"
}

Gonzales-Barron, U., Dijkshoorn, R., Maloncy, M., Finimundy, T., Calhelha, R. C., Pereira, C., Stojković, D., Soković, M., Ferreira, I. C. F. R., Barros, L.,& Cadavez, V.. (2020). Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.. in Foods (Basel, Switzerland)
MDPI Multidisciplinary Digital Publishing Institute., 9(5), 597.
https://doi.org/10.3390/foods9050597

Gonzales-Barron U, Dijkshoorn R, Maloncy M, Finimundy T, Calhelha RC, Pereira C, Stojković D, Soković M, Ferreira ICFR, Barros L, Cadavez V. Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking.. in Foods (Basel, Switzerland). 2020;9(5):597.
doi:10.3390/foods9050597 .

Gonzales-Barron, Ursula, Dijkshoorn, Rody, Maloncy, Maikel, Finimundy, Tiane, Calhelha, Ricardo C., Pereira, Carla, Stojković, Dejan, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, Cadavez, Vasco, "Nutritive and Bioactive Properties of Mesquite (Prosopis pallida) Flour and Its Technological Performance in Breadmaking." in Foods (Basel, Switzerland), 9, no. 5 (2020):597,
https://doi.org/10.3390/foods9050597 . .

TY  - JOUR
AU  - Finimundy, Tiane C.
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Caleja, Cristina
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Stojković, Dejan
AU  - Carvalho, Ana Maria
AU  - Rosa, Eduardo
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/9/2151
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3677
AB  - Several plants have been used for medicinal applications and have been traditionally consumed as decoctions and infusions. Although some herbs are used alone as a beverage, they are often blended in mixtures to maximize their effects. Herein, the nutritional characterization of six infusions from herbal blends was evaluated using the official methods of analysis (AOAC international). A further characterization of the individual phenolic profile was also performed by HPLC-DAD/ESI-MSn, and finally bioactive potential was determined by evaluating the antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities of each blend. The wide variety of plants in each sample led to variability in the results for all analyzed parameters. However, blends containing 15% Laurus nobilis L. and 15% Juglan regia L. in their composition showed higher sugar content and energy contribution; higher concentration of phenolic compounds (phenolic acids and flavonoids); greater antioxidant, cytotoxic, and anti-inflammatory capacity; and also better antimicrobial effects against all the tested bacterial and fungal strains. Further studies will be necessary to evaluate the real synergistic effects that these two species show in the presence of other plants, and to evaluate their potential for application in various food, pharmaceutical, and nutraceutical products as infusion preparations.
PB  - NLM (Medline)
T2  - Molecules
T1  - Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties
IS  - 9
VL  - 25
DO  - 10.3390/molecules25092151
SP  - 2151
ER  - 

@article{
author = "Finimundy, Tiane C. and Pereira, Carla and Dias, Maria Inês and Caleja, Cristina and Calhelha, Ricardo C. and Soković, Marina and Stojković, Dejan and Carvalho, Ana Maria and Rosa, Eduardo and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "Several plants have been used for medicinal applications and have been traditionally consumed as decoctions and infusions. Although some herbs are used alone as a beverage, they are often blended in mixtures to maximize their effects. Herein, the nutritional characterization of six infusions from herbal blends was evaluated using the official methods of analysis (AOAC international). A further characterization of the individual phenolic profile was also performed by HPLC-DAD/ESI-MSn, and finally bioactive potential was determined by evaluating the antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities of each blend. The wide variety of plants in each sample led to variability in the results for all analyzed parameters. However, blends containing 15% Laurus nobilis L. and 15% Juglan regia L. in their composition showed higher sugar content and energy contribution; higher concentration of phenolic compounds (phenolic acids and flavonoids); greater antioxidant, cytotoxic, and anti-inflammatory capacity; and also better antimicrobial effects against all the tested bacterial and fungal strains. Further studies will be necessary to evaluate the real synergistic effects that these two species show in the presence of other plants, and to evaluate their potential for application in various food, pharmaceutical, and nutraceutical products as infusion preparations.",
publisher = "NLM (Medline)",
journal = "Molecules",
title = "Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties",
number = "9",
volume = "25",
doi = "10.3390/molecules25092151",
pages = "2151"
}

Finimundy, T. C., Pereira, C., Dias, M. I., Caleja, C., Calhelha, R. C., Soković, M., Stojković, D., Carvalho, A. M., Rosa, E., Barros, L.,& Ferreira, I. C. F. R.. (2020). Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties. in Molecules
NLM (Medline)., 25(9), 2151.
https://doi.org/10.3390/molecules25092151

Finimundy TC, Pereira C, Dias MI, Caleja C, Calhelha RC, Soković M, Stojković D, Carvalho AM, Rosa E, Barros L, Ferreira ICFR. Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties. in Molecules. 2020;25(9):2151.
doi:10.3390/molecules25092151 .

Finimundy, Tiane C., Pereira, Carla, Dias, Maria Inês, Caleja, Cristina, Calhelha, Ricardo C., Soković, Marina, Stojković, Dejan, Carvalho, Ana Maria, Rosa, Eduardo, Barros, Lillian, Ferreira, Isabel C. F. R., "Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties" in Molecules, 25, no. 9 (2020):2151,
https://doi.org/10.3390/molecules25092151 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Ines
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/9/2204
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3675
AB  - The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.
PB  - MDPI AG
T2  - Molecules
T1  - Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions
IS  - 9
VL  - 25
DO  - 10.3390/molecules25092204
SP  - 2204
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Ines and Pereira, Carla and Calhelha, Ricardo C. and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.",
publisher = "MDPI AG",
journal = "Molecules",
title = "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions",
number = "9",
volume = "25",
doi = "10.3390/molecules25092204",
pages = "2204"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Chrysargyris, A., Tzortzakis, N., Ivanov, M., Soković, M., Barros, L.,& Ferreira, I. C. F. R.. (2020). Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules
MDPI AG., 25(9), 2204.
https://doi.org/10.3390/molecules25092204

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Chrysargyris A, Tzortzakis N, Ivanov M, Soković M, Barros L, Ferreira ICFR. Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules. 2020;25(9):2204.
doi:10.3390/molecules25092204 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Ines, Pereira, Carla, Calhelha, Ricardo C., Chrysargyris, Antonios, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C. F. R., "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions" in Molecules, 25, no. 9 (2020):2204,
https://doi.org/10.3390/molecules25092204 . .