TY  - CONF
AU  - Krstić-Milošević, Dijana
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Devrnja, Nina
AU  - Eler, Klemen
AU  - Vinterhalter, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5560
AB  - Gentiana clusii Perr. & Song. (Gentianaceae), is a rare plant species found in the Alps, the Pyrenees, the Black Forest Mountains and the Carpathians. G. clusii is endemic for Europe which implies the need for its conservation. G. clusii plants were collected at fruitful stage in June 2015 at Velika Planina Mountain, Republic of Slovenia. Seeds of G. clusii were germinated on MS basal medium. Shoots cultured on WPM + 0.5 mg/L KIN were used as starting material in propagation and rooting experiments. Medium with IBA at 0.5 mg/L was chosen as optimal for rooting. HPLC screening of secondary metabolites of wild plants collected in nature and in vitro plants revealed the presence of two main classes of compounds typical for gentians – secoiridoids and xanthones. Secoiridoids gentiopicrin and sweroside were identified in both aerial parts of wild plants and shoots of in vitro plants, while root extracts contained amarogentin in addition. Aerial parts of wild plants contained gentiacaulein-1-O-primeveroside as dominant xanthone glycoside. HPLC also revealed the presence of gentiakochianin-1-O-primeveroside, norswertianin-1-O-primeveroside, 1,8- dihydroxy-3-methoxy-7-O-primeverosyl xanthone, and xanthone aglycons- norswertianin, gentiacaulein, and gentiakochianin. HPLC analysis shown that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg/L KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside for more than 2-fold compared with the wild plants. IBA promoted norswertianin-1-O-primeveroside and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed high antioxidant capacity. The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
T1  - Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5560
ER  - 

@conference{
editor = "Baebler, Špela, Dermastia, Marina, Grebenc, Tine, Praprotnik, Eva, Razinger, Jaka, Urbanek Krajnc, Andreja",
author = "Krstić-Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Devrnja, Nina and Eler, Klemen and Vinterhalter, Branka",
year = "2022",
abstract = "Gentiana clusii Perr. & Song. (Gentianaceae), is a rare plant species found in the Alps, the Pyrenees, the Black Forest Mountains and the Carpathians. G. clusii is endemic for Europe which implies the need for its conservation. G. clusii plants were collected at fruitful stage in June 2015 at Velika Planina Mountain, Republic of Slovenia. Seeds of G. clusii were germinated on MS basal medium. Shoots cultured on WPM + 0.5 mg/L KIN were used as starting material in propagation and rooting experiments. Medium with IBA at 0.5 mg/L was chosen as optimal for rooting. HPLC screening of secondary metabolites of wild plants collected in nature and in vitro plants revealed the presence of two main classes of compounds typical for gentians – secoiridoids and xanthones. Secoiridoids gentiopicrin and sweroside were identified in both aerial parts of wild plants and shoots of in vitro plants, while root extracts contained amarogentin in addition. Aerial parts of wild plants contained gentiacaulein-1-O-primeveroside as dominant xanthone glycoside. HPLC also revealed the presence of gentiakochianin-1-O-primeveroside, norswertianin-1-O-primeveroside, 1,8- dihydroxy-3-methoxy-7-O-primeverosyl xanthone, and xanthone aglycons- norswertianin, gentiacaulein, and gentiakochianin. HPLC analysis shown that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg/L KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside for more than 2-fold compared with the wild plants. IBA promoted norswertianin-1-O-primeveroside and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed high antioxidant capacity. The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia",
title = "Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5560"
}

Baebler, Š., Dermastia, M., Grebenc, T., Praprotnik, E., Razinger, J., Urbanek Krajnc, A., Krstić-Milošević, D., Banjac, N., Janković, T., Devrnja, N., Eler, K.,& Vinterhalter, B.. (2022). Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia
Ljubljana: Slovenian Society of Plant Biology., 31.
https://hdl.handle.net/21.15107/rcub_ibiss_5560

Baebler Š, Dermastia M, Grebenc T, Praprotnik E, Razinger J, Urbanek Krajnc A, Krstić-Milošević D, Banjac N, Janković T, Devrnja N, Eler K, Vinterhalter B. Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. 2022;:31.
https://hdl.handle.net/21.15107/rcub_ibiss_5560 .

Baebler, Špela, Dermastia, Marina, Grebenc, Tine, Praprotnik, Eva, Razinger, Jaka, Urbanek Krajnc, Andreja, Krstić-Milošević, Dijana, Banjac, Nevena, Janković, Teodora, Devrnja, Nina, Eler, Klemen, Vinterhalter, Branka, "Gentiana clusii Perr.&Song. cultured in vitro - secondary metabolite profile and evaluation of antioxidant activity" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia (2022):31,
https://hdl.handle.net/21.15107/rcub_ibiss_5560 .

TY  - CONF
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Despotović, Sanja
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Stojanović, Ivana D.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5783
AB  - Many plant extracts are well known for their anti-oxidant, anti-bacterial and antiinflammatory
activities including Aronia berry-derived juices and powders. In
comparison to other black berries, Aronia berries have a greater content of phenolic
constituents such as procyanidins, anthocyanins and phenolic acids with antioxidative
and anti-inflammatory properties. However, the effects of aronia berries extract on the
immune response parameters have been only sporadically assessed. When administered
orally to healthy C57BL/6 mice (50 mg/kg body weight), aronia extract exerted
immunomodulatory effects as evidenced by decreased proportion of F4/80+
macrophages, CD11c+ dendritic cells, CD4+ T helper cells, CD8+ T cytotoxic
lymphocytes and CD4+CD25- activated lymphocytes within the gut-associated
lymphoid tissue. Surprisingly, oral consumption of chokeberry extract in doses of either
200 mg/kg bw or 50 mg/kg bw in mice with multiple low dose streptozotocin-induced
type 1 diabetes resulted in the increase of blood glucose levels. Further, our study shows
that this detrimental effect on type 1 diabetes pathogenesis may be a consequence of
the pro-inflammatory nature of the extract. This is based on the evident stimulation of
macrophages and dendritic cells by the extract through up-regulation of proinflammatory
mediators such as nitric oxide, IL-12, IL-6 and TNF in vitro. Also, this
extract augmented differentiation of IFN-γ-producing T helper 1 cells in vitro.
Collectively, the obtained results imply that our particular aronia berries fruit extract
displays pro-inflammatory characteristics and that care should be taken when these
berries are to be included in the human diet.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia
T1  - Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5783
ER  - 

@conference{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Vujičić, Milica and Despotović, Sanja and Šavikin, Katarina and Janković, Teodora and Stojanović, Ivana D.",
year = "2021",
abstract = "Many plant extracts are well known for their anti-oxidant, anti-bacterial and antiinflammatory
activities including Aronia berry-derived juices and powders. In
comparison to other black berries, Aronia berries have a greater content of phenolic
constituents such as procyanidins, anthocyanins and phenolic acids with antioxidative
and anti-inflammatory properties. However, the effects of aronia berries extract on the
immune response parameters have been only sporadically assessed. When administered
orally to healthy C57BL/6 mice (50 mg/kg body weight), aronia extract exerted
immunomodulatory effects as evidenced by decreased proportion of F4/80+
macrophages, CD11c+ dendritic cells, CD4+ T helper cells, CD8+ T cytotoxic
lymphocytes and CD4+CD25- activated lymphocytes within the gut-associated
lymphoid tissue. Surprisingly, oral consumption of chokeberry extract in doses of either
200 mg/kg bw or 50 mg/kg bw in mice with multiple low dose streptozotocin-induced
type 1 diabetes resulted in the increase of blood glucose levels. Further, our study shows
that this detrimental effect on type 1 diabetes pathogenesis may be a consequence of
the pro-inflammatory nature of the extract. This is based on the evident stimulation of
macrophages and dendritic cells by the extract through up-regulation of proinflammatory
mediators such as nitric oxide, IL-12, IL-6 and TNF in vitro. Also, this
extract augmented differentiation of IFN-γ-producing T helper 1 cells in vitro.
Collectively, the obtained results imply that our particular aronia berries fruit extract
displays pro-inflammatory characteristics and that care should be taken when these
berries are to be included in the human diet.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia",
title = "Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5783"
}

Mićanović, D., Saksida, T., Koprivica, I., Vujičić, M., Despotović, S., Šavikin, K., Janković, T.,& Stojanović, I. D.. (2021). Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo. in Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_5783

Mićanović D, Saksida T, Koprivica I, Vujičić M, Despotović S, Šavikin K, Janković T, Stojanović ID. Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo. in Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia. 2021;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_5783 .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Vujičić, Milica, Despotović, Sanja, Šavikin, Katarina, Janković, Teodora, Stojanović, Ivana D., "Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo" in Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia (2021):130,
https://hdl.handle.net/21.15107/rcub_ibiss_5783 .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4310
AB  - In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established from epicotyl explants cultured on MS basal medium with 0.2 mg L−1 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L−1 indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-O-glucoside and bellidifolin-8-O-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-O-glucoside, and bellidifolin-8-O-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of G. lutescens and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.
PB  - Basel: MDPI
T2  - Plants
T1  - Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production
IS  - 8
VL  - 10
DO  - 10.3390/plants10081651
SP  - 1651
ER  - 

@article{
author = "Krstić Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2021",
abstract = "In vitro shoot culture of the endangered medicinal plant Gentianella lutescens was established from epicotyl explants cultured on MS basal medium with 0.2 mg L−1 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L−1 indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-O-glucoside and bellidifolin-8-O-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-O-glucoside, and bellidifolin-8-O-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of G. lutescens and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production",
number = "8",
volume = "10",
doi = "10.3390/plants10081651",
pages = "1651"
}

Krstić Milošević, D., Banjac, N., Janković, T., Vinterhalter, D.,& Vinterhalter, B.. (2021). Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production. in Plants
Basel: MDPI., 10(8), 1651.
https://doi.org/10.3390/plants10081651

Krstić Milošević D, Banjac N, Janković T, Vinterhalter D, Vinterhalter B. Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production. in Plants. 2021;10(8):1651.
doi:10.3390/plants10081651 .

Krstić Milošević, Dijana, Banjac, Nevena, Janković, Teodora, Vinterhalter, Dragan, Vinterhalter, Branka, "Gentianella lutescens subsp. carpatica J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production" in Plants, 10, no. 8 (2021):1651,
https://doi.org/10.3390/plants10081651 . .

TY  - JOUR
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Despotović, Sanja
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Stojanović, Ivana D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3591
AB  - Chokeberry (Aronia melanocarpa) is known for its anti-oxidant, anti-inflammatory and anti-diabetic properties. Since the effects of chokeberry extract on the immune response have been only sporadically assessed, our aim was to investigate chokeberry fruit water extract on the immune response in vivo and in vitro. When administered orally to healthy mice, the extract exerted immunomodulatory effects in the gut evidenced by the altered proportion of macrophages, dendritic cells and T cells. Importantly, oral consumption of the chokeberry extract resulted in blood glucose level increase in C57BL/6 mice with chemically-induced diabetes. These in vivo results were corroborated by observed up-regulation of nitric oxide and interelukin-1β production in macrophages and dendritic cells, up-regulated phagocytic activity of macrophages, increased T and B lymphocytes proportions and differentiation of interferon-γ-producing T cells in vitro. The obtained results imply that our chokeberry extract stimulates pro-inflammatory properties in immune cells of innate and adaptive immunity.
PB  - Elsevier Ltd.
T2  - Journal of Functional Foods
T1  - Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro
VL  - 66
DO  - 10.1016/j.jff.2020.103836
SP  - 103836
ER  - 

@article{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Vujičić, Milica and Despotović, Sanja and Šavikin, Katarina and Janković, Teodora and Stojanović, Ivana D.",
year = "2020",
abstract = "Chokeberry (Aronia melanocarpa) is known for its anti-oxidant, anti-inflammatory and anti-diabetic properties. Since the effects of chokeberry extract on the immune response have been only sporadically assessed, our aim was to investigate chokeberry fruit water extract on the immune response in vivo and in vitro. When administered orally to healthy mice, the extract exerted immunomodulatory effects in the gut evidenced by the altered proportion of macrophages, dendritic cells and T cells. Importantly, oral consumption of the chokeberry extract resulted in blood glucose level increase in C57BL/6 mice with chemically-induced diabetes. These in vivo results were corroborated by observed up-regulation of nitric oxide and interelukin-1β production in macrophages and dendritic cells, up-regulated phagocytic activity of macrophages, increased T and B lymphocytes proportions and differentiation of interferon-γ-producing T cells in vitro. The obtained results imply that our chokeberry extract stimulates pro-inflammatory properties in immune cells of innate and adaptive immunity.",
publisher = "Elsevier Ltd.",
journal = "Journal of Functional Foods",
title = "Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro",
volume = "66",
doi = "10.1016/j.jff.2020.103836",
pages = "103836"
}

Mićanović, D., Saksida, T., Koprivica, I., Vujičić, M., Despotović, S., Šavikin, K., Janković, T.,& Stojanović, I. D.. (2020). Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro. in Journal of Functional Foods
Elsevier Ltd.., 66, 103836.
https://doi.org/10.1016/j.jff.2020.103836

Mićanović D, Saksida T, Koprivica I, Vujičić M, Despotović S, Šavikin K, Janković T, Stojanović ID. Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro. in Journal of Functional Foods. 2020;66:103836.
doi:10.1016/j.jff.2020.103836 .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Vujičić, Milica, Despotović, Sanja, Šavikin, Katarina, Janković, Teodora, Stojanović, Ivana D., "Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro" in Journal of Functional Foods, 66 (2020):103836,
https://doi.org/10.1016/j.jff.2020.103836 . .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Eler, Klemen
AU  - Vinterhalter, Branka
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32763798
UR  - https://radar.ibiss.bg.ac.rs/123456789/3847
AB  - Shoot and root in vitro culture of endemic European species Gentiana clusii was established for the first time. The effects of different concentrations of benzyl adenine (BA), 6-phurphurylaminopurine (KIN), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on shoot propagation and rooting of G. clusii were investigated. The optimal in vitro conditions for shoot propagation and long-term maintenance were achieved using woody plant medium (WPM) supplemented with 0.5 mg l-1 KIN, and subsequent application of IBA at 0.5 mg l-1 significantly improved rooting of these shoots. Root culture was established from excised root tips cultured in ½ MS liquid media with increasing concentrations of IBA (0.1-1.0 mg l-1). A high root growth rate and considerable biomass yield were obtained by addition of 1.0 mg l-1 IBA. HPLC analysis revealed that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg l-1 KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside (N-1-P) for more than 2-fold compared with the wild plants. IBA promoted N-1-P and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed the highest antioxidant capacity (IC50 = 66.57 μg ml-1). The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.
PB  - Elsevier Masson SAS
T2  - Plant Physiology and Biochemistry
T1  - Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.
VL  - 154
DO  - 10.1016/j.plaphy.2020.07.007
SP  - 735
EP  - 744
ER  - 

@article{
author = "Krstić Milošević, Dijana and Banjac, Nevena and Janković, Teodora and Eler, Klemen and Vinterhalter, Branka",
year = "2020",
abstract = "Shoot and root in vitro culture of endemic European species Gentiana clusii was established for the first time. The effects of different concentrations of benzyl adenine (BA), 6-phurphurylaminopurine (KIN), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on shoot propagation and rooting of G. clusii were investigated. The optimal in vitro conditions for shoot propagation and long-term maintenance were achieved using woody plant medium (WPM) supplemented with 0.5 mg l-1 KIN, and subsequent application of IBA at 0.5 mg l-1 significantly improved rooting of these shoots. Root culture was established from excised root tips cultured in ½ MS liquid media with increasing concentrations of IBA (0.1-1.0 mg l-1). A high root growth rate and considerable biomass yield were obtained by addition of 1.0 mg l-1 IBA. HPLC analysis revealed that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg l-1 KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside (N-1-P) for more than 2-fold compared with the wild plants. IBA promoted N-1-P and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed the highest antioxidant capacity (IC50 = 66.57 μg ml-1). The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones.",
publisher = "Elsevier Masson SAS",
journal = "Plant Physiology and Biochemistry",
title = "Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.",
volume = "154",
doi = "10.1016/j.plaphy.2020.07.007",
pages = "735-744"
}

Krstić Milošević, D., Banjac, N., Janković, T., Eler, K.,& Vinterhalter, B.. (2020). Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.. in Plant Physiology and Biochemistry
Elsevier Masson SAS., 154, 735-744.
https://doi.org/10.1016/j.plaphy.2020.07.007

Krstić Milošević D, Banjac N, Janković T, Eler K, Vinterhalter B. Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.. in Plant Physiology and Biochemistry. 2020;154:735-744.
doi:10.1016/j.plaphy.2020.07.007 .

Krstić Milošević, Dijana, Banjac, Nevena, Janković, Teodora, Eler, Klemen, Vinterhalter, Branka, "Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation." in Plant Physiology and Biochemistry, 154 (2020):735-744,
https://doi.org/10.1016/j.plaphy.2020.07.007 . .

TY  - CONF
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Despotović, Sanja
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Stojanović, Ivana D.
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5777
AB  - Chokeberry (Aronia melanocarpa) is known for its strong anti-oxidant properties. Antiinflammatory,
anti-hypertensive and anti-diabetogenic activities of orally consumed
chokeberry extracts have also been reported. The effects of chokeberry extract on the
immune response parameters have been only sporadically assessed. Therefore, the aim of
our study was to investigate the effects of orally consumed chokeberry extract on the
immune response in vivo and in vitro in healthy and in diabetic C57BL/6 mice, in which
diabetes was induced by multiple low doses of streptozotocin (MLDS). Chokeberry extract
administered to healthy mice (50 mg/kg body weight) exerted immunomodulatory effects
as evidenced by decreased proportion of F4/80+ macrophages, CD11c+ dendritic cells,
CD4+ T helper cells, CD8+ T cytotoxic lymphocytes and CD4+CD25- activated T
lymphocytes within the gut-associated lymphoid tissue. Surprisingly, oral consumption of
chokeberry extract in doses of either 200 mg/kg bw or 50 mg/kg bw in diabetic mice
resulted in the increase of blood glucose levels. In an attempt to decipher the underlying
mechanisms of chokeberry extract effects in the context of autoimmune/inflammatory
disease, we have evaluated its effects in vitro on purified immune cells. Seemingly, the
chokeberry extract exerted pro-inflammatory effects in vitro through the up-regulation of
nitric oxide and IL-1β production in macrophages and dendritic cells, increased
macrophage CD86-related activation and promotion of type 1 T helper cells (IFN-γ+)
differentiation. In addition, an increased proportion of CD4+, CD8+ and B lymphocytes
within the spleen was observed. Collectively, the obtained results imply that our particular
chokeberry extract displays pro-inflammatory characteristics and that care should be taken
when chokeberry is to be included in the human diet.
PB  - Belgrade: Faculty of Chemistry
C3  - The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.
T1  - Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5777
ER  - 

@conference{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Vujičić, Milica and Despotović, Sanja and Šavikin, Katarina and Janković, Teodora and Stojanović, Ivana D.",
year = "2019",
abstract = "Chokeberry (Aronia melanocarpa) is known for its strong anti-oxidant properties. Antiinflammatory,
anti-hypertensive and anti-diabetogenic activities of orally consumed
chokeberry extracts have also been reported. The effects of chokeberry extract on the
immune response parameters have been only sporadically assessed. Therefore, the aim of
our study was to investigate the effects of orally consumed chokeberry extract on the
immune response in vivo and in vitro in healthy and in diabetic C57BL/6 mice, in which
diabetes was induced by multiple low doses of streptozotocin (MLDS). Chokeberry extract
administered to healthy mice (50 mg/kg body weight) exerted immunomodulatory effects
as evidenced by decreased proportion of F4/80+ macrophages, CD11c+ dendritic cells,
CD4+ T helper cells, CD8+ T cytotoxic lymphocytes and CD4+CD25- activated T
lymphocytes within the gut-associated lymphoid tissue. Surprisingly, oral consumption of
chokeberry extract in doses of either 200 mg/kg bw or 50 mg/kg bw in diabetic mice
resulted in the increase of blood glucose levels. In an attempt to decipher the underlying
mechanisms of chokeberry extract effects in the context of autoimmune/inflammatory
disease, we have evaluated its effects in vitro on purified immune cells. Seemingly, the
chokeberry extract exerted pro-inflammatory effects in vitro through the up-regulation of
nitric oxide and IL-1β production in macrophages and dendritic cells, increased
macrophage CD86-related activation and promotion of type 1 T helper cells (IFN-γ+)
differentiation. In addition, an increased proportion of CD4+, CD8+ and B lymphocytes
within the spleen was observed. Collectively, the obtained results imply that our particular
chokeberry extract displays pro-inflammatory characteristics and that care should be taken
when chokeberry is to be included in the human diet.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.",
title = "Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5777"
}

Mićanović, D., Saksida, T., Koprivica, I., Vujičić, M., Despotović, S., Šavikin, K., Janković, T.,& Stojanović, I. D.. (2019). Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro. in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.
Belgrade: Faculty of Chemistry., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5777

Mićanović D, Saksida T, Koprivica I, Vujičić M, Despotović S, Šavikin K, Janković T, Stojanović ID. Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro. in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.. 2019;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5777 .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Vujičić, Milica, Despotović, Sanja, Šavikin, Katarina, Janković, Teodora, Stojanović, Ivana D., "Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro" in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia. (2019):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5777 .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Uzelac, Branka
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2017
UR  - https://link.springer.com/article/10.1007/s11240-017-1252-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2773
AB  - The effect of abiotic (salicylic acid, jasmonic acid and methyl jasmonate) and biotic (chitosan and yeast extract) elicitors on the growth and xanthone accumulation in two hairy root clones of Gentiana dinarica Beck. was studied. The obtained results showed that clone 3 was more responsive to elicitor treatment than clone D. The production of dominant xanthone norswertianin-1-O-primeveroside was not significantly affected by either of the abiotic elicitor tested but was stimulated with chitosan treatment. The highest concentrations of all elicitors strongly increased the content of xanthone aglycone norswertianin, but simultaneously reducing the production of its glycoside norswertianin-1-O-primeveroside. The most efficient in enhancing norswertianin production was a 7-day treatment with salicylic acid (200 μM) and chitosan (50 mg l− 1), which yielded a 7.7- and a 24-fold increase in norswertianin content, respectively. In addition, treatment with biotic elicitors caused the occurrence of new xanthone compounds that were not detected in other samples. Free radical scavenging activity of xanthones was carried out by DPPH assay, and norswertianin showed the strongest activity.
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica
VL  - 130
DO  - 10.1007/s11240-017-1252-1
SP  - 631
EP  - 640
ER  - 

@article{
author = "Krstić Milošević, Dijana and Janković, Teodora and Uzelac, Branka and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2017",
abstract = "The effect of abiotic (salicylic acid, jasmonic acid and methyl jasmonate) and biotic (chitosan and yeast extract) elicitors on the growth and xanthone accumulation in two hairy root clones of Gentiana dinarica Beck. was studied. The obtained results showed that clone 3 was more responsive to elicitor treatment than clone D. The production of dominant xanthone norswertianin-1-O-primeveroside was not significantly affected by either of the abiotic elicitor tested but was stimulated with chitosan treatment. The highest concentrations of all elicitors strongly increased the content of xanthone aglycone norswertianin, but simultaneously reducing the production of its glycoside norswertianin-1-O-primeveroside. The most efficient in enhancing norswertianin production was a 7-day treatment with salicylic acid (200 μM) and chitosan (50 mg l− 1), which yielded a 7.7- and a 24-fold increase in norswertianin content, respectively. In addition, treatment with biotic elicitors caused the occurrence of new xanthone compounds that were not detected in other samples. Free radical scavenging activity of xanthones was carried out by DPPH assay, and norswertianin showed the strongest activity.",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica",
volume = "130",
doi = "10.1007/s11240-017-1252-1",
pages = "631-640"
}

Krstić Milošević, D., Janković, T., Uzelac, B., Vinterhalter, D.,& Vinterhalter, B.. (2017). Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica. in Plant Cell, Tissue and Organ Culture (PCTOC), 130, 631-640.
https://doi.org/10.1007/s11240-017-1252-1

Krstić Milošević D, Janković T, Uzelac B, Vinterhalter D, Vinterhalter B. Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica. in Plant Cell, Tissue and Organ Culture (PCTOC). 2017;130:631-640.
doi:10.1007/s11240-017-1252-1 .

Krstić Milošević, Dijana, Janković, Teodora, Uzelac, Branka, Vinterhalter, Dragan, Vinterhalter, Branka, "Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica" in Plant Cell, Tissue and Organ Culture (PCTOC), 130 (2017):631-640,
https://doi.org/10.1007/s11240-017-1252-1 . .

TY  - CHAP
AU  - Krstić Milošević, Dijana
AU  - Vinterhalter, Branka
AU  - Janković, Teodora
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2571
UR  - http://link.springer.com/chapter/10.1007%2F978-3-642-54102-5_4
AB  - In the central regions of the Balkan Peninsula, the genus Gentianella is represented by six species. Five of them G. albanica, G. austriaca, G. bulgarica, G. ciliata, and G. crispata were subject of phytochemical and in vitro studies. Two species, G. austriaca and G. bulgarica, were established as shoot cultures and procedures developed for their in vitro propagation. Difficulties with seed germination and precocious flowering affected the final propagation stages. Phytochemical analyses were performed on five species from nature and on cultured shoots. Plant material was rich in secondary metabolites with xanthones, flavone-C-glucosides, and secoiridoids as the principal constituents. The xanthone aglycones, demethy- bellidifolin, bellidifolin, corymbiferin, and their corresponding glucosides were dominant components in all the species investigated. The predominant substitution pattern of xanthones found in the genus Gentianella was 1,3,5,8 and to a lesser extent, 1,3,7,8. Lanceoside and veratriloside were new compounds found in European Gentianella species. In vitro material generally contained less secondary metabolites than plants from nature; the content of secondary products was influenced by the concentration of benzyladenine (BAP) in the culture medium.
PB  - Springer Berlin Heidelberg, Berlin, Heidelberg
T2  - The Gentianaceae - Volume 2: Biotechnology and Applications
T1  - Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula
DO  - 10.1007/978-3-642-54102-5
SP  - 93
EP  - 112
ER  - 

@inbook{
editor = "Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna",
author = "Krstić Milošević, Dijana and Vinterhalter, Branka and Janković, Teodora and Vinterhalter, Dragan",
year = "2015",
abstract = "In the central regions of the Balkan Peninsula, the genus Gentianella is represented by six species. Five of them G. albanica, G. austriaca, G. bulgarica, G. ciliata, and G. crispata were subject of phytochemical and in vitro studies. Two species, G. austriaca and G. bulgarica, were established as shoot cultures and procedures developed for their in vitro propagation. Difficulties with seed germination and precocious flowering affected the final propagation stages. Phytochemical analyses were performed on five species from nature and on cultured shoots. Plant material was rich in secondary metabolites with xanthones, flavone-C-glucosides, and secoiridoids as the principal constituents. The xanthone aglycones, demethy- bellidifolin, bellidifolin, corymbiferin, and their corresponding glucosides were dominant components in all the species investigated. The predominant substitution pattern of xanthones found in the genus Gentianella was 1,3,5,8 and to a lesser extent, 1,3,7,8. Lanceoside and veratriloside were new compounds found in European Gentianella species. In vitro material generally contained less secondary metabolites than plants from nature; the content of secondary products was influenced by the concentration of benzyladenine (BAP) in the culture medium.",
publisher = "Springer Berlin Heidelberg, Berlin, Heidelberg",
journal = "The Gentianaceae - Volume 2: Biotechnology and Applications",
booktitle = "Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula",
doi = "10.1007/978-3-642-54102-5",
pages = "93-112"
}

Rybczyński, J. J., Davey, M. R., Mikuła, A., Krstić Milošević, D., Vinterhalter, B., Janković, T.,& Vinterhalter, D.. (2015). Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula. in The Gentianaceae - Volume 2: Biotechnology and Applications
Springer Berlin Heidelberg, Berlin, Heidelberg., 93-112.
https://doi.org/10.1007/978-3-642-54102-5

Rybczyński JJ, Davey MR, Mikuła A, Krstić Milošević D, Vinterhalter B, Janković T, Vinterhalter D. Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula. in The Gentianaceae - Volume 2: Biotechnology and Applications. 2015;:93-112.
doi:10.1007/978-3-642-54102-5 .

Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna, Krstić Milošević, Dijana, Vinterhalter, Branka, Janković, Teodora, Vinterhalter, Dragan, "Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula" in The Gentianaceae - Volume 2: Biotechnology and Applications (2015):93-112,
https://doi.org/10.1007/978-3-642-54102-5 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Zivković, Jelena
AU  - Soković, Marina
AU  - Ferreira, Isabel CFR
AU  - Janković, Teodora
AU  - Maksimović, Zoran A
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1017
AB  - This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system
IS  - null
VL  - 55
SP  - 39
EP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1017
ER  - 

@article{
author = "Stojković, Dejan and Glamočlija, Jasmina and Zivković, Jelena and Soković, Marina and Ferreira, Isabel CFR and Janković, Teodora and Maksimović, Zoran A",
year = "2013",
abstract = "This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system",
number = "null",
volume = "55",
pages = "39-213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1017"
}

Stojković, D., Glamočlija, J., Zivković, J., Soković, M., Ferreira, I. C., Janković, T.,& Maksimović, Z. A.. (2013). Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology, 55(null), 39-213.
https://hdl.handle.net/21.15107/rcub_ibiss_1017

Stojković D, Glamočlija J, Zivković J, Soković M, Ferreira IC, Janković T, Maksimović ZA. Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology. 2013;55(null):39-213.
https://hdl.handle.net/21.15107/rcub_ibiss_1017 .

Stojković, Dejan, Glamočlija, Jasmina, Zivković, Jelena, Soković, Marina, Ferreira, Isabel CFR, Janković, Teodora, Maksimović, Zoran A, "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system" in Food and Chemical Toxicology, 55, no. null (2013):39-213,
https://hdl.handle.net/21.15107/rcub_ibiss_1017 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan V
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1042
AB  - Gentiana dinarica Beck, native to the Balkan Dinaric Mountains, was established in vitro from axillary shoot buds. It was maintained in the form of shoot cultures on MS medium supplemented with 1.0 mg l(-1) 6-benzyladenine (BA) and 0.1 mg l(-1) alpha-naphthaleneacetic acid and excised root cultures were maintained on 1/2MS medium with 0.5 mg l(-1) indole-3-butyric acid (IBA). Shoot cultures, adventitious roots and excised root cultures were analysed by HPLC techniques for the presence of secoiridoids and xanthones. Gentiopicrin and swertiamarin, the dominant components of shoot cultures, could not be detected in root cultures. Xanthones were present in both shoot and root cultures with norswertianin-1-O-primeveroside as the dominant metabolite. The secoiridoid and xanthone content, although characteristic for certain plant organs, was dependent on the concentration of plant growth regulators (BA and IBA) added to the medium. BA in the shoot multiplication stage strongly increased the secondary metabolite (SEM) content of shoot cultures. IBA had little effect on SEM accumulation in shoots during rooting, while it moderately stimulated SEM accumulation in excised root cultures.
T2  - Acta Physiologiae Plantarum
T1  - Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro
IS  - 2
VL  - 35
SP  - 3
EP  - 574
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1042
ER  - 

@article{
author = "Vinterhalter, Branka and Krstić Milošević, Dijana and Janković, Teodora and Zdravković-Korać, Snežana and Vinterhalter, Dragan V",
year = "2013",
abstract = "Gentiana dinarica Beck, native to the Balkan Dinaric Mountains, was established in vitro from axillary shoot buds. It was maintained in the form of shoot cultures on MS medium supplemented with 1.0 mg l(-1) 6-benzyladenine (BA) and 0.1 mg l(-1) alpha-naphthaleneacetic acid and excised root cultures were maintained on 1/2MS medium with 0.5 mg l(-1) indole-3-butyric acid (IBA). Shoot cultures, adventitious roots and excised root cultures were analysed by HPLC techniques for the presence of secoiridoids and xanthones. Gentiopicrin and swertiamarin, the dominant components of shoot cultures, could not be detected in root cultures. Xanthones were present in both shoot and root cultures with norswertianin-1-O-primeveroside as the dominant metabolite. The secoiridoid and xanthone content, although characteristic for certain plant organs, was dependent on the concentration of plant growth regulators (BA and IBA) added to the medium. BA in the shoot multiplication stage strongly increased the secondary metabolite (SEM) content of shoot cultures. IBA had little effect on SEM accumulation in shoots during rooting, while it moderately stimulated SEM accumulation in excised root cultures.",
journal = "Acta Physiologiae Plantarum",
title = "Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro",
number = "2",
volume = "35",
pages = "3-574",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1042"
}

Vinterhalter, B., Krstić Milošević, D., Janković, T., Zdravković-Korać, S.,& Vinterhalter, D. V.. (2013). Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro. in Acta Physiologiae Plantarum, 35(2), 3-574.
https://hdl.handle.net/21.15107/rcub_ibiss_1042

Vinterhalter B, Krstić Milošević D, Janković T, Zdravković-Korać S, Vinterhalter DV. Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro. in Acta Physiologiae Plantarum. 2013;35(2):3-574.
https://hdl.handle.net/21.15107/rcub_ibiss_1042 .

Vinterhalter, Branka, Krstić Milošević, Dijana, Janković, Teodora, Zdravković-Korać, Snežana, Vinterhalter, Dragan V, "Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro" in Acta Physiologiae Plantarum, 35, no. 2 (2013):3-574,
https://hdl.handle.net/21.15107/rcub_ibiss_1042 .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Vinterhalter, Branka
AU  - Menković, Nebojsa R
AU  - Aljancić, Ivana S
AU  - Vinterhalter, Dragan V
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/955
AB  - The effects of different types and concentrations of sugars on root growth and xanthone production in root culture of Gentiana dinarica were investigated. The results showed that sucrose, glucose and fructose all supported root growth, and sucrose was superior in terms of growth index, dry mass and fresh/dry mass ratio then fructose or glucose at the same concentrations. However, considering equimolar concentration of sugars, their contribution to the root growth was similar. The HPLC analysis of roots indicated the presence of xanthone compounds, and the contents of norswertianin-1-O-primeveroside (1), norswertianin-1-O-glucoside (2), gentioside (3) and norswertianin (4) were evaluated. In all samples, norswertianin-1-O-primeveroside (1) was present in highest concentration, followed by norswertianin-1-O-glucoside (2), whereas gentioside (3) and norswertianin (4) were present in lower amounts. The production of xanthones was affected by both type and concentration of sugar. In general, roots growing in media supplemented with sucrose contained higher levels of xanthones. The amounts of xanthone primeveroses (1) and (3) increased with the increase of concentrations of all types of sugars, whereas higher sugar concentrations resulted in reduction of the contents of norswertianin-1-O-glucoside (2) and aglycone norswertianin (4). The roots were also evaluated regarding the content of total phenolics and higher accumulation of total phenolic compounds was observed in roots grown in fructose-containing medium. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and high correlation between total phenolic content and antiradical activity was observed (r = -0.83).
T2  - Plant Growth Regulation
T1  - Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck
IS  - 2
VL  - 71
SP  - 15
EP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_955
ER  - 

@article{
author = "Krstić Milošević, Dijana and Janković, Teodora and Vinterhalter, Branka and Menković, Nebojsa R and Aljancić, Ivana S and Vinterhalter, Dragan V",
year = "2013",
abstract = "The effects of different types and concentrations of sugars on root growth and xanthone production in root culture of Gentiana dinarica were investigated. The results showed that sucrose, glucose and fructose all supported root growth, and sucrose was superior in terms of growth index, dry mass and fresh/dry mass ratio then fructose or glucose at the same concentrations. However, considering equimolar concentration of sugars, their contribution to the root growth was similar. The HPLC analysis of roots indicated the presence of xanthone compounds, and the contents of norswertianin-1-O-primeveroside (1), norswertianin-1-O-glucoside (2), gentioside (3) and norswertianin (4) were evaluated. In all samples, norswertianin-1-O-primeveroside (1) was present in highest concentration, followed by norswertianin-1-O-glucoside (2), whereas gentioside (3) and norswertianin (4) were present in lower amounts. The production of xanthones was affected by both type and concentration of sugar. In general, roots growing in media supplemented with sucrose contained higher levels of xanthones. The amounts of xanthone primeveroses (1) and (3) increased with the increase of concentrations of all types of sugars, whereas higher sugar concentrations resulted in reduction of the contents of norswertianin-1-O-glucoside (2) and aglycone norswertianin (4). The roots were also evaluated regarding the content of total phenolics and higher accumulation of total phenolic compounds was observed in roots grown in fructose-containing medium. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and high correlation between total phenolic content and antiradical activity was observed (r = -0.83).",
journal = "Plant Growth Regulation",
title = "Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck",
number = "2",
volume = "71",
pages = "15-155",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_955"
}

Krstić Milošević, D., Janković, T., Vinterhalter, B., Menković, N. R., Aljancić, I. S.,& Vinterhalter, D. V.. (2013). Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck. in Plant Growth Regulation, 71(2), 15-155.
https://hdl.handle.net/21.15107/rcub_ibiss_955

Krstić Milošević D, Janković T, Vinterhalter B, Menković NR, Aljancić IS, Vinterhalter DV. Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck. in Plant Growth Regulation. 2013;71(2):15-155.
https://hdl.handle.net/21.15107/rcub_ibiss_955 .

Krstić Milošević, Dijana, Janković, Teodora, Vinterhalter, Branka, Menković, Nebojsa R, Aljancić, Ivana S, Vinterhalter, Dragan V, "Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck" in Plant Growth Regulation, 71, no. 2 (2013):15-155,
https://hdl.handle.net/21.15107/rcub_ibiss_955 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Milojević, Jelena
AU  - Vinterhalter, Dragan V
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1158
AB  - Gentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l(-1) BA and 0.1 mg l(-1) NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5-1.0 mg l(-1) IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.
T2  - Central European Journal of Biology
T1  - In vitro propagation of Gentiana dinarica Beck.
IS  - 4
VL  - 7
EP  - 697
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1158
ER  - 

@article{
author = "Vinterhalter, Branka and Krstić Milošević, Dijana and Janković, Teodora and Milojević, Jelena and Vinterhalter, Dragan V",
year = "2012",
abstract = "Gentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l(-1) BA and 0.1 mg l(-1) NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5-1.0 mg l(-1) IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.",
journal = "Central European Journal of Biology",
title = "In vitro propagation of Gentiana dinarica Beck.",
number = "4",
volume = "7",
pages = "697",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1158"
}

Vinterhalter, B., Krstić Milošević, D., Janković, T., Milojević, J.,& Vinterhalter, D. V.. (2012). In vitro propagation of Gentiana dinarica Beck.. in Central European Journal of Biology, 7(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1158

Vinterhalter B, Krstić Milošević D, Janković T, Milojević J, Vinterhalter DV. In vitro propagation of Gentiana dinarica Beck.. in Central European Journal of Biology. 2012;7(4):null-697.
https://hdl.handle.net/21.15107/rcub_ibiss_1158 .

Vinterhalter, Branka, Krstić Milošević, Dijana, Janković, Teodora, Milojević, Jelena, Vinterhalter, Dragan V, "In vitro propagation of Gentiana dinarica Beck." in Central European Journal of Biology, 7, no. 4 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1158 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Janković, Teodora
AU  - Miljuš-Đukić, Jovanka
AU  - Vinterhalter, Dragan
PY  - 2011
UR  - https://bp.ueb.cas.cz/pdfs/bpl/2011/03/02.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6167
AB  - Differences among three clones of Gentiana punctata L. hairy root shoot regenerants were investigated in relation to their growth patterns, production of secondary metabolites and 2D protein profiles. Prominent differences in growth parameters were stable thus qualifying regenerant clones as true somaclones. Marked differences in protein spots were registered among the regenerant clones but not in comparison with the non-transformed control. Southern blot hybridization of regenerants showed the absence of rolA, B and C genes, initially present in the main hairy root lines. Orf13 and rolD were present and orf8 was missing in all three regenerant clones whereas orf3 was missing only in clone 2. Although lacking the three major rol genes, plants of regenerant clones retained characteristics of the hairy root phenotype.
PB  - Dordrecht: Springer-Verlag
T2  - Biologia Plantarum
T1  - Variability in shoot cultures of hairy root regenerated Gentiana punctata L.
IS  - 3
VL  - 55
DO  - 10.1007/s10535-011-0105-8
SP  - 414
EP  - 422
ER  - 

@article{
author = "Vinterhalter, Branka and Zdravković-Korać, Snežana and Ninković, Slavica and Banjac, Nevena and Janković, Teodora and Miljuš-Đukić, Jovanka and Vinterhalter, Dragan",
year = "2011",
abstract = "Differences among three clones of Gentiana punctata L. hairy root shoot regenerants were investigated in relation to their growth patterns, production of secondary metabolites and 2D protein profiles. Prominent differences in growth parameters were stable thus qualifying regenerant clones as true somaclones. Marked differences in protein spots were registered among the regenerant clones but not in comparison with the non-transformed control. Southern blot hybridization of regenerants showed the absence of rolA, B and C genes, initially present in the main hairy root lines. Orf13 and rolD were present and orf8 was missing in all three regenerant clones whereas orf3 was missing only in clone 2. Although lacking the three major rol genes, plants of regenerant clones retained characteristics of the hairy root phenotype.",
publisher = "Dordrecht: Springer-Verlag",
journal = "Biologia Plantarum",
title = "Variability in shoot cultures of hairy root regenerated Gentiana punctata L.",
number = "3",
volume = "55",
doi = "10.1007/s10535-011-0105-8",
pages = "414-422"
}

Vinterhalter, B., Zdravković-Korać, S., Ninković, S., Banjac, N., Janković, T., Miljuš-Đukić, J.,& Vinterhalter, D.. (2011). Variability in shoot cultures of hairy root regenerated Gentiana punctata L.. in Biologia Plantarum
Dordrecht: Springer-Verlag., 55(3), 414-422.
https://doi.org/10.1007/s10535-011-0105-8

Vinterhalter B, Zdravković-Korać S, Ninković S, Banjac N, Janković T, Miljuš-Đukić J, Vinterhalter D. Variability in shoot cultures of hairy root regenerated Gentiana punctata L.. in Biologia Plantarum. 2011;55(3):414-422.
doi:10.1007/s10535-011-0105-8 .

Vinterhalter, Branka, Zdravković-Korać, Snežana, Ninković, Slavica, Banjac, Nevena, Janković, Teodora, Miljuš-Đukić, Jovanka, Vinterhalter, Dragan, "Variability in shoot cultures of hairy root regenerated Gentiana punctata L." in Biologia Plantarum, 55, no. 3 (2011):414-422,
https://doi.org/10.1007/s10535-011-0105-8 . .

TY  - JOUR
AU  - Janković, Teodora
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Nikolić, Radomirka R
AU  - Vinterhalter, Dragan V
AU  - Milosavljević, Slobodan M
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1282
AB  - Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).
T2  - Acta Physiologiae Plantarum
T1  - Xanthone compounds in shoot cultures of Gentianella bulgarica
IS  - 4
VL  - 33
EP  - 1520
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1282
ER  - 

@article{
author = "Janković, Teodora and Vinterhalter, Branka and Krstić Milošević, Dijana and Nikolić, Radomirka R and Vinterhalter, Dragan V and Milosavljević, Slobodan M",
year = "2011",
abstract = "Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).",
journal = "Acta Physiologiae Plantarum",
title = "Xanthone compounds in shoot cultures of Gentianella bulgarica",
number = "4",
volume = "33",
pages = "1520",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1282"
}

Janković, T., Vinterhalter, B., Krstić Milošević, D., Nikolić, R. R., Vinterhalter, D. V.,& Milosavljević, S. M.. (2011). Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum, 33(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1282

Janković T, Vinterhalter B, Krstić Milošević D, Nikolić RR, Vinterhalter DV, Milosavljević SM. Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum. 2011;33(4):null-1520.
https://hdl.handle.net/21.15107/rcub_ibiss_1282 .

Janković, Teodora, Vinterhalter, Branka, Krstić Milošević, Dijana, Nikolić, Radomirka R, Vinterhalter, Dragan V, Milosavljević, Slobodan M, "Xanthone compounds in shoot cultures of Gentianella bulgarica" in Acta Physiologiae Plantarum, 33, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1282 .

TY  - JOUR
AU  - Janković, Teodora
AU  - Krstić Milošević, Dijana
AU  - Aljancić, Ivana S
AU  - Savikin, Katarina P.
AU  - Menković, Nebojsa R
AU  - Radanović, Dragoja S
AU  - Milosavljević, Slobodan M
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1484
AB  - Xanthone-O-glycosides with 1,3,7,8-oxidation pattern and flavone isoorientin-3'-O-glucoside were isolated from the aerial parts of Gentiana utriculosa. Xanthone-C-glucoside lancerin was detected in Gentiana species for the first time. The distribution of these compounds within the section Calathianae is discussed.
T2  - Natural Product Research
T1  - Phytochemical re-investigation of Gentiana utriculosa
IS  - 5
VL  - 23
EP  - 469
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1484
ER  - 

@article{
author = "Janković, Teodora and Krstić Milošević, Dijana and Aljancić, Ivana S and Savikin, Katarina P. and Menković, Nebojsa R and Radanović, Dragoja S and Milosavljević, Slobodan M",
year = "2009",
abstract = "Xanthone-O-glycosides with 1,3,7,8-oxidation pattern and flavone isoorientin-3'-O-glucoside were isolated from the aerial parts of Gentiana utriculosa. Xanthone-C-glucoside lancerin was detected in Gentiana species for the first time. The distribution of these compounds within the section Calathianae is discussed.",
journal = "Natural Product Research",
title = "Phytochemical re-investigation of Gentiana utriculosa",
number = "5",
volume = "23",
pages = "469",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1484"
}

Janković, T., Krstić Milošević, D., Aljancić, I. S., Savikin, K. P., Menković, N. R., Radanović, D. S.,& Milosavljević, S. M.. (2009). Phytochemical re-investigation of Gentiana utriculosa. in Natural Product Research, 23(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1484

Janković T, Krstić Milošević D, Aljancić IS, Savikin KP, Menković NR, Radanović DS, Milosavljević SM. Phytochemical re-investigation of Gentiana utriculosa. in Natural Product Research. 2009;23(5):null-469.
https://hdl.handle.net/21.15107/rcub_ibiss_1484 .

Janković, Teodora, Krstić Milošević, Dijana, Aljancić, Ivana S, Savikin, Katarina P., Menković, Nebojsa R, Radanović, Dragoja S, Milosavljević, Slobodan M, "Phytochemical re-investigation of Gentiana utriculosa" in Natural Product Research, 23, no. 5 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1484 .

TY  - JOUR
AU  - Isaković, Aleksandra J
AU  - Janković, Teodora
AU  - Harhaji-Trajković, Ljubica
AU  - Kostić-Rajačić, Slađana V.
AU  - Nikolić, Zoran M
AU  - Vajs, Vlatka E
AU  - Trajković, Vladimir S
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1530
AB  - The present study identifies xanthones gentiakochianin and gentiacaulein as the active principles responsible for the in vitro antiglioma action of ether and methanolic extracts of the plant Gentiana kochiana. Gentiakochianin and gentiacaulein induced cell cycle arrest in G(2)/M and G(0)/G(1) phases, respectively, in both C6 rat glioma and U251 human glioma cell lines. The more efficient antiproliferative action of gentiakochianin was associated with its ability to induce microtubule stabilization in a cell-free assay. Both the xanthones reduced mitochondrial membrane potential and increased the production of reactive oxygen species in glioma cells, but only the effects of gentiakochianin were pronounced enough to cause caspase activation and subsequent apoptotic cell death. The assessment of structure-activity relationship in a series of structurally related xanthones from G. kochiana and Gentianella austriaca revealed dihydroxylation at positions 7, 8 of the xanthonic nucleus as the key structural feature responsible for the ability of gentiakochianin to induce microtubule-associated G(2)/M cell block and apoptotic cell death in glioma cells. (C) 2008 Elsevier Ltd. All rights reserved.
T2  - Bioorganic & Medicinal Chemistry
T1  - Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements
IS  - 10
VL  - 16
EP  - 5694
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1530
ER  - 

@article{
author = "Isaković, Aleksandra J and Janković, Teodora and Harhaji-Trajković, Ljubica and Kostić-Rajačić, Slađana V. and Nikolić, Zoran M and Vajs, Vlatka E and Trajković, Vladimir S",
year = "2008",
abstract = "The present study identifies xanthones gentiakochianin and gentiacaulein as the active principles responsible for the in vitro antiglioma action of ether and methanolic extracts of the plant Gentiana kochiana. Gentiakochianin and gentiacaulein induced cell cycle arrest in G(2)/M and G(0)/G(1) phases, respectively, in both C6 rat glioma and U251 human glioma cell lines. The more efficient antiproliferative action of gentiakochianin was associated with its ability to induce microtubule stabilization in a cell-free assay. Both the xanthones reduced mitochondrial membrane potential and increased the production of reactive oxygen species in glioma cells, but only the effects of gentiakochianin were pronounced enough to cause caspase activation and subsequent apoptotic cell death. The assessment of structure-activity relationship in a series of structurally related xanthones from G. kochiana and Gentianella austriaca revealed dihydroxylation at positions 7, 8 of the xanthonic nucleus as the key structural feature responsible for the ability of gentiakochianin to induce microtubule-associated G(2)/M cell block and apoptotic cell death in glioma cells. (C) 2008 Elsevier Ltd. All rights reserved.",
journal = "Bioorganic & Medicinal Chemistry",
title = "Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements",
number = "10",
volume = "16",
pages = "5694",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1530"
}

Isaković, A. J., Janković, T., Harhaji-Trajković, L., Kostić-Rajačić, S. V., Nikolić, Z. M., Vajs, V. E.,& Trajković, V. S.. (2008). Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements. in Bioorganic & Medicinal Chemistry, 16(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1530

Isaković AJ, Janković T, Harhaji-Trajković L, Kostić-Rajačić SV, Nikolić ZM, Vajs VE, Trajković VS. Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements. in Bioorganic & Medicinal Chemistry. 2008;16(10):null-5694.
https://hdl.handle.net/21.15107/rcub_ibiss_1530 .

Isaković, Aleksandra J, Janković, Teodora, Harhaji-Trajković, Ljubica, Kostić-Rajačić, Slađana V., Nikolić, Zoran M, Vajs, Vlatka E, Trajković, Vladimir S, "Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements" in Bioorganic & Medicinal Chemistry, 16, no. 10 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1530 .

TY  - JOUR
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
AU  - Janković, Teodora
AU  - Krstić, Dijana
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/167
AB  - Austrian gentian, Gentianella austriaca (A. Kern. & Jos. Kern), Gentianaceae [syn. Gentiana germanica Willd. subsp. Austriaca] is endemic alpine plant populated at altitudes above 1500 m and up to 2800 m (Struwe et al. 2002). It may be also found in central mountains of Serbia, over 2000m. Although a rare mountain plant G. austriaca is used in traditional medicine to stimulate appetite and to treat digestive complaints, like the other bitter gentians. It is poorly pharmacologically explored, albeit it contains yellow pigments - xanthones, a group of plant secondary metabolites.
T2  - Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
T1  - Neurohemijska in vito aktivnost ksantona Gentianella austriaca
T1  - Neurochemical in vitro activity of xanthones from Gentianella austriaca
IS  - 2
VL  - 41
SP  - 83
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_167
ER  - 

@article{
author = "Tovilović-Kovačević, Gordana and Tomić, Mirko and Janković, Teodora and Krstić, Dijana",
year = "2005, 2005",
abstract = "Austrian gentian, Gentianella austriaca (A. Kern. & Jos. Kern), Gentianaceae [syn. Gentiana germanica Willd. subsp. Austriaca] is endemic alpine plant populated at altitudes above 1500 m and up to 2800 m (Struwe et al. 2002). It may be also found in central mountains of Serbia, over 2000m. Although a rare mountain plant G. austriaca is used in traditional medicine to stimulate appetite and to treat digestive complaints, like the other bitter gentians. It is poorly pharmacologically explored, albeit it contains yellow pigments - xanthones, a group of plant secondary metabolites.",
journal = "Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta",
title = "Neurohemijska in vito aktivnost ksantona Gentianella austriaca, Neurochemical in vitro activity of xanthones from Gentianella austriaca",
number = "2",
volume = "41",
pages = "83-86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_167"
}

Tovilović-Kovačević, G., Tomić, M., Janković, T.,& Krstić, D.. (2005). Neurohemijska in vito aktivnost ksantona Gentianella austriaca. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 41(2), 83-86.
https://hdl.handle.net/21.15107/rcub_ibiss_167

Tovilović-Kovačević G, Tomić M, Janković T, Krstić D. Neurohemijska in vito aktivnost ksantona Gentianella austriaca. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta. 2005;41(2):83-86.
https://hdl.handle.net/21.15107/rcub_ibiss_167 .

Tovilović-Kovačević, Gordana, Tomić, Mirko, Janković, Teodora, Krstić, Dijana, "Neurohemijska in vito aktivnost ksantona Gentianella austriaca" in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 41, no. 2 (2005):83-86,
https://hdl.handle.net/21.15107/rcub_ibiss_167 .

TY  - JOUR
AU  - Janković, Teodora
AU  - Krstić, Dijana
AU  - Aljancić, Ivana S
AU  - Savikin-Fodulović, Katarina P.
AU  - Menković, Nebojsa R
AU  - Vajs, Vlatka E
AU  - Milosavljević, Slobodan M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1698
T2  - Biochemical Systematics and Ecology
T1  - Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro
IS  - 7
VL  - 33
EP  - 735
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1698
ER  - 

@article{
author = "Janković, Teodora and Krstić, Dijana and Aljancić, Ivana S and Savikin-Fodulović, Katarina P. and Menković, Nebojsa R and Vajs, Vlatka E and Milosavljević, Slobodan M",
year = "2005",
journal = "Biochemical Systematics and Ecology",
title = "Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro",
number = "7",
volume = "33",
pages = "735",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1698"
}

Janković, T., Krstić, D., Aljancić, I. S., Savikin-Fodulović, K. P., Menković, N. R., Vajs, V. E.,& Milosavljević, S. M.. (2005). Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro. in Biochemical Systematics and Ecology, 33(7).
https://hdl.handle.net/21.15107/rcub_ibiss_1698

Janković T, Krstić D, Aljancić IS, Savikin-Fodulović KP, Menković NR, Vajs VE, Milosavljević SM. Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro. in Biochemical Systematics and Ecology. 2005;33(7):null-735.
https://hdl.handle.net/21.15107/rcub_ibiss_1698 .

Janković, Teodora, Krstić, Dijana, Aljancić, Ivana S, Savikin-Fodulović, Katarina P., Menković, Nebojsa R, Vajs, Vlatka E, Milosavljević, Slobodan M, "Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro" in Biochemical Systematics and Ecology, 33, no. 7 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1698 .

TY  - JOUR
AU  - Krstić, Dijana
AU  - Janković, Teodora
AU  - Aljancić, Ivana S
AU  - Savikin-Fodulović, Katarina P.
AU  - Menković, Nebojsa R
AU  - Milosavljević, Slobodan M
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1724
T2  - Biochemical Systematics and Ecology
T1  - Phytochemical investigation of Gentiana dinarica
IS  - 10
VL  - 32
EP  - 941
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1724
ER  - 

@article{
author = "Krstić, Dijana and Janković, Teodora and Aljancić, Ivana S and Savikin-Fodulović, Katarina P. and Menković, Nebojsa R and Milosavljević, Slobodan M",
year = "2004",
journal = "Biochemical Systematics and Ecology",
title = "Phytochemical investigation of Gentiana dinarica",
number = "10",
volume = "32",
pages = "941",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1724"
}

Krstić, D., Janković, T., Aljancić, I. S., Savikin-Fodulović, K. P., Menković, N. R.,& Milosavljević, S. M.. (2004). Phytochemical investigation of Gentiana dinarica. in Biochemical Systematics and Ecology, 32(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1724

Krstić D, Janković T, Aljancić IS, Savikin-Fodulović KP, Menković NR, Milosavljević SM. Phytochemical investigation of Gentiana dinarica. in Biochemical Systematics and Ecology. 2004;32(10):null-941.
https://hdl.handle.net/21.15107/rcub_ibiss_1724 .

Krstić, Dijana, Janković, Teodora, Aljancić, Ivana S, Savikin-Fodulović, Katarina P., Menković, Nebojsa R, Milosavljević, Slobodan M, "Phytochemical investigation of Gentiana dinarica" in Biochemical Systematics and Ecology, 32, no. 10 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1724 .

TY  - JOUR
AU  - Šavikin-Fodulović, Katarina
AU  - Stojanović, Danilo
AU  - Menković, Nebojša
AU  - Krstić, Dijana
AU  - Janković, Teodora
PY  - 2001
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/402
AB  - Gentiana dinarica Beck. (Gentianaceae) is a rare plant species growing at carbonate soils in subalpine and alpine regions. Acording to the Flora of Serbia G. dinarica is only distributed in Kosovo. In course of our recent pharmacognostic investigations G. dinarica was found at two localities on the mountain Tara, locality Zvezda (1230 m) and Veliki Kraj (1330 m). Plant material (aerial parts and roots) was collected from June to August 2000-2001. Anatomic and phytochemical analyses were carried out. Preliminary screening of the extracts of the roots and aerial parts of G. dinarica showed the presence of secoiridoids, flavonoids and xanthone compounds. From both extracts three secoiridoids were isolated (swertiamarin, gentiopicrin and sweroside).
AB  - Vrsta Gentiana dinarica Beck. (Gentianaceae) je retka biljna vrsta koja je u Srbiji, prema Flori Srbije, do sada konstatovana samo na Kosovu. U toku terenskih istraživanja, ovu vrstu smo zabeležili na dva lokaliteta na planini Tari i to lokalitet Zvezda (1230 m) i lokalitet Veliki Kraj (1330 m).Specifičnost anatomske građe korena G. dinarica je slabo odrveneo kislem, i tzv. unutarksilemski floem, odnosno grupice floemskih elemenata u centralnom delu sekundarnog drveta.Preliminarno ispitivanje ekstrakata korena i nadzemnog dela G. dinarica pokazao je prisustvo sekoiridoida, flavonoida i ksantona. Od sekoiridoida, izolovani su svercijamarin, genciopikrin i sverozid.
T2  - Lekovite sirovine
T1  - Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae
T1  - Phytochemical and anatomic study of plant species Gentiana dinarica Beck
IS  - 21
VL  - null
SP  - 31
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_402
ER  - 

@article{
author = "Šavikin-Fodulović, Katarina and Stojanović, Danilo and Menković, Nebojša and Krstić, Dijana and Janković, Teodora",
year = "2001, 2001",
abstract = "Gentiana dinarica Beck. (Gentianaceae) is a rare plant species growing at carbonate soils in subalpine and alpine regions. Acording to the Flora of Serbia G. dinarica is only distributed in Kosovo. In course of our recent pharmacognostic investigations G. dinarica was found at two localities on the mountain Tara, locality Zvezda (1230 m) and Veliki Kraj (1330 m). Plant material (aerial parts and roots) was collected from June to August 2000-2001. Anatomic and phytochemical analyses were carried out. Preliminary screening of the extracts of the roots and aerial parts of G. dinarica showed the presence of secoiridoids, flavonoids and xanthone compounds. From both extracts three secoiridoids were isolated (swertiamarin, gentiopicrin and sweroside)., Vrsta Gentiana dinarica Beck. (Gentianaceae) je retka biljna vrsta koja je u Srbiji, prema Flori Srbije, do sada konstatovana samo na Kosovu. U toku terenskih istraživanja, ovu vrstu smo zabeležili na dva lokaliteta na planini Tari i to lokalitet Zvezda (1230 m) i lokalitet Veliki Kraj (1330 m).Specifičnost anatomske građe korena G. dinarica je slabo odrveneo kislem, i tzv. unutarksilemski floem, odnosno grupice floemskih elemenata u centralnom delu sekundarnog drveta.Preliminarno ispitivanje ekstrakata korena i nadzemnog dela G. dinarica pokazao je prisustvo sekoiridoida, flavonoida i ksantona. Od sekoiridoida, izolovani su svercijamarin, genciopikrin i sverozid.",
journal = "Lekovite sirovine",
title = "Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae, Phytochemical and anatomic study of plant species Gentiana dinarica Beck",
number = "21",
volume = "null",
pages = "31-36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_402"
}

Šavikin-Fodulović, K., Stojanović, D., Menković, N., Krstić, D.,& Janković, T.. (2001). Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae. in Lekovite sirovine, null(21), 31-36.
https://hdl.handle.net/21.15107/rcub_ibiss_402

Šavikin-Fodulović K, Stojanović D, Menković N, Krstić D, Janković T. Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae. in Lekovite sirovine. 2001;null(21):31-36.
https://hdl.handle.net/21.15107/rcub_ibiss_402 .

Šavikin-Fodulović, Katarina, Stojanović, Danilo, Menković, Nebojša, Krstić, Dijana, Janković, Teodora, "Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae" in Lekovite sirovine, null, no. 21 (2001):31-36,
https://hdl.handle.net/21.15107/rcub_ibiss_402 .