Milojević, Jelena

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orcid::0000-0001-8822-9771
  • Milojević, Jelena (43)
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Author's Bibliography

Recent advances in somatic embryogenesis induction in Aesculus species

Zdravković-Korać, Snežana; Ćalić, Dušica; Milojević, Jelena; Belić, Maja; Jevremović, Slađana

(COST Action CA21157 COPYTREE, 2024)

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Jevremović, Slađana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6713
AB  - Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.
PB  - COST Action CA21157 COPYTREE
C3  - Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
T1  - Recent advances in somatic embryogenesis induction in Aesculus species
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6713
ER  - 
@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Jevremović, Slađana",
year = "2024",
abstract = "Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.",
publisher = "COST Action CA21157 COPYTREE",
journal = "Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia",
title = "Recent advances in somatic embryogenesis induction in Aesculus species",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6713"
}
Zdravković-Korać, S., Ćalić, D., Milojević, J., Belić, M.,& Jevremović, S.. (2024). Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
COST Action CA21157 COPYTREE., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713
Zdravković-Korać S, Ćalić D, Milojević J, Belić M, Jevremović S. Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia. 2024;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .
Zdravković-Korać, Snežana, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Jevremović, Slađana, "Recent advances in somatic embryogenesis induction in Aesculus species" in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia (2024):38,
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .

Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static Magnetic Fields

Ćalić, Dušica; Ristić-Đurović, Jasna L.; Ćirković, Saša; Milojević, Jelena; Belić, Maja; Stanišić, Mariana; Zdravković-Korać, Snežana

(Basel: MDPI, 2023)

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Ristić-Đurović, Jasna L.
AU  - Ćirković, Saša
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Stanišić, Mariana
AU  - Zdravković-Korać, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6420
AB  - Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions
PB  - Basel: MDPI
T2  - Agriculture
T1  - Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields
IS  - 11
VL  - 13
DO  - 10.3390/agriculture13112120
SP  - 2120
ER  - 
@article{
author = "Ćalić, Dušica and Ristić-Đurović, Jasna L. and Ćirković, Saša and Milojević, Jelena and Belić, Maja and Stanišić, Mariana and Zdravković-Korać, Snežana",
year = "2023",
abstract = "Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields",
number = "11",
volume = "13",
doi = "10.3390/agriculture13112120",
pages = "2120"
}
Ćalić, D., Ristić-Đurović, J. L., Ćirković, S., Milojević, J., Belić, M., Stanišić, M.,& Zdravković-Korać, S.. (2023). Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture
Basel: MDPI., 13(11), 2120.
https://doi.org/10.3390/agriculture13112120
Ćalić D, Ristić-Đurović JL, Ćirković S, Milojević J, Belić M, Stanišić M, Zdravković-Korać S. Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture. 2023;13(11):2120.
doi:10.3390/agriculture13112120 .
Ćalić, Dušica, Ristić-Đurović, Jasna L., Ćirković, Saša, Milojević, Jelena, Belić, Maja, Stanišić, Mariana, Zdravković-Korać, Snežana, "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields" in Agriculture, 13, no. 11 (2023):2120,
https://doi.org/10.3390/agriculture13112120 . .
1

Somatic Embryogenesis in Spinach—A Review

Zdravković-Korać, Snežana; Belić, Maja; Ćalić, Dušica; Milojević, Jelena

(Basel: MDPI, 2023)

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6090
AB  - A spinach-supplemented diet exerts numerous health benefits, but high levels of oxalic acid and nitrate can cause medical problems, so their levels should be reduced, while the levels of vitamins and phytochemicals could be further increased by breeding. Conventional spinach breeding is limited by the very complex sex determination. However, these limitations could be circumvented in synergy with a biotechnological approach. Accordingly, tissue culture techniques allow rapid and efficient clonal propagation of selected valuable genotypes, and somatic embryogenesis has been recognized as a superior process for clonal propagation because somatic embryos resemble zygotic embryos and therefore can spontaneously develop into complete plants. Since spinach has been considered recalcitrant to in vitro regeneration for decades, a deeper insight into the mechanisms underlying somatic embryogenesis is important for a better understanding and further improvement of the efficiency of this process. In this review, a comprehensive overview of the major factors affecting somatic embryogenesis in spinach is presented and discussed, with particular
emphasis on the synergistic effects of α–naphthaleneacetic acid, gibberellic acid, light, and the intrinsic predisposition of individual seedlings to somatic embryogenesis, as well as the expression of genes encoding key enzymes involved in the maintenance of gibberellin homeostasis and the levels of endogenous gibberellins.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Somatic Embryogenesis in Spinach—A Review
IS  - 9
VL  - 9
DO  - 10.3390/horticulturae9091048
SP  - 1048
ER  - 
@article{
author = "Zdravković-Korać, Snežana and Belić, Maja and Ćalić, Dušica and Milojević, Jelena",
year = "2023",
abstract = "A spinach-supplemented diet exerts numerous health benefits, but high levels of oxalic acid and nitrate can cause medical problems, so their levels should be reduced, while the levels of vitamins and phytochemicals could be further increased by breeding. Conventional spinach breeding is limited by the very complex sex determination. However, these limitations could be circumvented in synergy with a biotechnological approach. Accordingly, tissue culture techniques allow rapid and efficient clonal propagation of selected valuable genotypes, and somatic embryogenesis has been recognized as a superior process for clonal propagation because somatic embryos resemble zygotic embryos and therefore can spontaneously develop into complete plants. Since spinach has been considered recalcitrant to in vitro regeneration for decades, a deeper insight into the mechanisms underlying somatic embryogenesis is important for a better understanding and further improvement of the efficiency of this process. In this review, a comprehensive overview of the major factors affecting somatic embryogenesis in spinach is presented and discussed, with particular
emphasis on the synergistic effects of α–naphthaleneacetic acid, gibberellic acid, light, and the intrinsic predisposition of individual seedlings to somatic embryogenesis, as well as the expression of genes encoding key enzymes involved in the maintenance of gibberellin homeostasis and the levels of endogenous gibberellins.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Somatic Embryogenesis in Spinach—A Review",
number = "9",
volume = "9",
doi = "10.3390/horticulturae9091048",
pages = "1048"
}
Zdravković-Korać, S., Belić, M., Ćalić, D.,& Milojević, J.. (2023). Somatic Embryogenesis in Spinach—A Review. in Horticulturae
Basel: MDPI., 9(9), 1048.
https://doi.org/10.3390/horticulturae9091048
Zdravković-Korać S, Belić M, Ćalić D, Milojević J. Somatic Embryogenesis in Spinach—A Review. in Horticulturae. 2023;9(9):1048.
doi:10.3390/horticulturae9091048 .
Zdravković-Korać, Snežana, Belić, Maja, Ćalić, Dušica, Milojević, Jelena, "Somatic Embryogenesis in Spinach—A Review" in Horticulturae, 9, no. 9 (2023):1048,
https://doi.org/10.3390/horticulturae9091048 . .
1

Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach

Belić, Maja; Tarkowská, Danuše; Zdravković-Korać, Snežana; Milojević, Jelena

(Belgrade: Serbian Plant Physiology Society, 2022)

TY  - CONF
AU  - Belić, Maja
AU  - Tarkowská, Danuše
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5367
AB  - Gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) are essential for efficient somatic embryo (SE) induction from root apices of spinach seedlings, while neither is sufficient to provoke an embryogenic response. To gain insight into the role of gibberellins (GAs) in SE induction, the levels of bioactive GAs, their precursors and inactivation products, were determined in embryogenic and non-embryogenic explants, cultivated on medium supplemented with 20 μM NAA + 5 μM GA3 and 20 μM NAA, respectively, for 24 h and 7 days, and in control explants - root apices that were frozen immediately after isolation. The content of GAs was determined using ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The levels of biosynthetic GA precursors GA44, GA19 and GA20 were 7–18-fold higher in embryogenic explants, and only 1.1–2.8-fold higher in non-embryogenic explants compared to the control. In embryogenic explants, the levels of bioactive GA1, GA3, GA4 and GA7 increased 843-, 50433-, 60- and 136-fold, respectively, after 24 h, and remained high after 7 days of treatment. In non-embryogenic explants, only a transient increase of GA3 level (22-fold) was observed after 24 h of treatment. Expectedly, the products of GA2ox inactivation (especially GA8) were significantly higher in embryogenic than in non-embryogenic and control explants. The results evidence a positive impact of enhanced GA metabolism on the acquisition of embryogenic competence, confirming their important role in SE induction and indicating that the 13 hydroxylation pathway is a prevalent pathway of bioactive GA synthesis in embryogenic explants.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5367
ER  - 
@conference{
author = "Belić, Maja and Tarkowská, Danuše and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2022",
abstract = "Gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) are essential for efficient somatic embryo (SE) induction from root apices of spinach seedlings, while neither is sufficient to provoke an embryogenic response. To gain insight into the role of gibberellins (GAs) in SE induction, the levels of bioactive GAs, their precursors and inactivation products, were determined in embryogenic and non-embryogenic explants, cultivated on medium supplemented with 20 μM NAA + 5 μM GA3 and 20 μM NAA, respectively, for 24 h and 7 days, and in control explants - root apices that were frozen immediately after isolation. The content of GAs was determined using ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The levels of biosynthetic GA precursors GA44, GA19 and GA20 were 7–18-fold higher in embryogenic explants, and only 1.1–2.8-fold higher in non-embryogenic explants compared to the control. In embryogenic explants, the levels of bioactive GA1, GA3, GA4 and GA7 increased 843-, 50433-, 60- and 136-fold, respectively, after 24 h, and remained high after 7 days of treatment. In non-embryogenic explants, only a transient increase of GA3 level (22-fold) was observed after 24 h of treatment. Expectedly, the products of GA2ox inactivation (especially GA8) were significantly higher in embryogenic than in non-embryogenic and control explants. The results evidence a positive impact of enhanced GA metabolism on the acquisition of embryogenic competence, confirming their important role in SE induction and indicating that the 13 hydroxylation pathway is a prevalent pathway of bioactive GA synthesis in embryogenic explants.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5367"
}
Belić, M., Tarkowská, D., Zdravković-Korać, S.,& Milojević, J.. (2022). Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 26.
https://hdl.handle.net/21.15107/rcub_ibiss_5367
Belić M, Tarkowská D, Zdravković-Korać S, Milojević J. Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:26.
https://hdl.handle.net/21.15107/rcub_ibiss_5367 .
Belić, Maja, Tarkowská, Danuše, Zdravković-Korać, Snežana, Milojević, Jelena, "Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):26,
https://hdl.handle.net/21.15107/rcub_ibiss_5367 .

Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review

Zdravković-Korać, Snežana; Milojević, Jelena; Belić, Maja; Ćalić, Dušica

(Basel: MDPI, 2022)

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Ćalić, Dušica
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/277
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4777
AB  - Species of the genus Aesculus are very attractive woody ornamentals. Their organs contain numerous health-promoting phytochemicals. The most valuable of them—aescin—is used in commercial preparations for the treatment of venous insufficiency. The industrial source of aescin is horse chestnut seeds because the zygotic embryos are the main site of its accumulation. Horse chestnut somatic and zygotic embryos contain similar amount of aescin, hence somatic embryos could be exploited as an alternative source of aescin. Somatic embryogenesis, androgenesis and de novo shoot organogenesis were successfully achieved in several Aesculus species, as well as secondary somatic embryogenesis and shoot organogenesis, which enables mass production of embryos and shoots. In addition, an efficient method for cryopreservation of embryogenic tissue was established, assuring constant availability of the plant material. The developed methods are suitable for clonal propagation of elite specimens selected as the best aescin producers, the most attractive ornamentals or plants resistant to pests and diseases. These methods are also useful for molecular breeding purposes. Thus, in this review, the medicinal uses and a comprehensive survey of in vitro propagation methods established for Aesculus species, as well as the feasibility of in vitro production of aescin, are presented and discussed.
PB  - Basel: MDPI
T2  - Plants
T1  - Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review
IS  - 3
VL  - 11
DO  - 10.3390/plants11030277
SP  - 277
ER  - 
@article{
author = "Zdravković-Korać, Snežana and Milojević, Jelena and Belić, Maja and Ćalić, Dušica",
year = "2022",
abstract = "Species of the genus Aesculus are very attractive woody ornamentals. Their organs contain numerous health-promoting phytochemicals. The most valuable of them—aescin—is used in commercial preparations for the treatment of venous insufficiency. The industrial source of aescin is horse chestnut seeds because the zygotic embryos are the main site of its accumulation. Horse chestnut somatic and zygotic embryos contain similar amount of aescin, hence somatic embryos could be exploited as an alternative source of aescin. Somatic embryogenesis, androgenesis and de novo shoot organogenesis were successfully achieved in several Aesculus species, as well as secondary somatic embryogenesis and shoot organogenesis, which enables mass production of embryos and shoots. In addition, an efficient method for cryopreservation of embryogenic tissue was established, assuring constant availability of the plant material. The developed methods are suitable for clonal propagation of elite specimens selected as the best aescin producers, the most attractive ornamentals or plants resistant to pests and diseases. These methods are also useful for molecular breeding purposes. Thus, in this review, the medicinal uses and a comprehensive survey of in vitro propagation methods established for Aesculus species, as well as the feasibility of in vitro production of aescin, are presented and discussed.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review",
number = "3",
volume = "11",
doi = "10.3390/plants11030277",
pages = "277"
}
Zdravković-Korać, S., Milojević, J., Belić, M.,& Ćalić, D.. (2022). Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review. in Plants
Basel: MDPI., 11(3), 277.
https://doi.org/10.3390/plants11030277
Zdravković-Korać S, Milojević J, Belić M, Ćalić D. Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review. in Plants. 2022;11(3):277.
doi:10.3390/plants11030277 .
Zdravković-Korać, Snežana, Milojević, Jelena, Belić, Maja, Ćalić, Dušica, "Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review" in Plants, 11, no. 3 (2022):277,
https://doi.org/10.3390/plants11030277 . .
3
3

Elucidation of the mechanism underlying somatic embryo induction in spinach

Milojević, Jelena; Zdravković-Korać, Snežana; Belić, Maja

(Belgrade: Serbian Plant Physiology Society, 2022)

TY  - CONF
AU  - Milojević, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5368
AB  - Spinach is a dioecious plant species with complex sex determination, which limits the success of its classical breeding. Hence, a biotechnological approach has been recognized as a promising alternative. Elucidation of the mechanism underlying somatic embryo (SE) induction and improvement of its efficiency have been the main goals of many years of research in this plant species recalcitrant to in vitro regeneration. Results have evidenced that the interplay among auxin, gibberellins (GA) and light was crucial for the induction of somatic embryogenesis from the root apices. None of these factors can be substituted for each other and only the lines with inherited high embryogenic capacity could respond in the absence of any of them, but with a very limited efficiency. The highest embryogenic response was obtained from the explants cultivated on medium supplemented with 20 μM α naphthaleneacetic acid (NAA) + 5 μM gibberellic acid (GA3) under blue light. Contrary to expectations, paclobutrazol, an inhibitor of bioactive gibberellin biosynthesis, acted synergistically with NAA and GA3 and further promoted somatic embryogenesis. In embryogenic explants, the expression levels of genes encoding the key enzymes involved in GA biosynthesis decreased, while those of genes involved in GA inactivation increased compared to non-embryogenic ones. Analysis of GA content using ultra-high performance liquid chromatography revealed enhanced GA metabolism in embryogenic explants during the first week of SE induction. During this period, the levels of endogenous bioactive GAs (GA1, GA3, GA4 and GA7) dramatically increased, confirming their important role in the acquisition of embryogenic potential.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Elucidation of the mechanism underlying somatic embryo induction in spinach
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5368
ER  - 
@conference{
author = "Milojević, Jelena and Zdravković-Korać, Snežana and Belić, Maja",
year = "2022",
abstract = "Spinach is a dioecious plant species with complex sex determination, which limits the success of its classical breeding. Hence, a biotechnological approach has been recognized as a promising alternative. Elucidation of the mechanism underlying somatic embryo (SE) induction and improvement of its efficiency have been the main goals of many years of research in this plant species recalcitrant to in vitro regeneration. Results have evidenced that the interplay among auxin, gibberellins (GA) and light was crucial for the induction of somatic embryogenesis from the root apices. None of these factors can be substituted for each other and only the lines with inherited high embryogenic capacity could respond in the absence of any of them, but with a very limited efficiency. The highest embryogenic response was obtained from the explants cultivated on medium supplemented with 20 μM α naphthaleneacetic acid (NAA) + 5 μM gibberellic acid (GA3) under blue light. Contrary to expectations, paclobutrazol, an inhibitor of bioactive gibberellin biosynthesis, acted synergistically with NAA and GA3 and further promoted somatic embryogenesis. In embryogenic explants, the expression levels of genes encoding the key enzymes involved in GA biosynthesis decreased, while those of genes involved in GA inactivation increased compared to non-embryogenic ones. Analysis of GA content using ultra-high performance liquid chromatography revealed enhanced GA metabolism in embryogenic explants during the first week of SE induction. During this period, the levels of endogenous bioactive GAs (GA1, GA3, GA4 and GA7) dramatically increased, confirming their important role in the acquisition of embryogenic potential.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Elucidation of the mechanism underlying somatic embryo induction in spinach",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5368"
}
Milojević, J., Zdravković-Korać, S.,& Belić, M.. (2022). Elucidation of the mechanism underlying somatic embryo induction in spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 134.
https://hdl.handle.net/21.15107/rcub_ibiss_5368
Milojević J, Zdravković-Korać S, Belić M. Elucidation of the mechanism underlying somatic embryo induction in spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:134.
https://hdl.handle.net/21.15107/rcub_ibiss_5368 .
Milojević, Jelena, Zdravković-Korać, Snežana, Belić, Maja, "Elucidation of the mechanism underlying somatic embryo induction in spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):134,
https://hdl.handle.net/21.15107/rcub_ibiss_5368 .

Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach

Belić, Maja; Zdravković-Korać, Snežana; Milojević, Jelena

(Belgrade: Serbian Plant Physiology Society, 2022)

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5366
AB  - Gibberellic acid (GA3) is indispensable for somatic embryo (SE) induction from root apices of spinach. To elucidate its role in SE induction, paclobutrazol (PAC), an inhibitor of gibberellin biosynthesis, was used. Root apices (1 cm) isolated from SE-derived, in vitro-cultivated plants of a previously selected line with high embryogenic capacity were used for experimentation. The explants were cultivated on media containing 20 μM α-naphthaleneacetic acid (NAA) + 0 or 5 μM GA3 + 0, 1, 2.5, 5 or 10 μM PAC. NAA alone induced SE regeneration in 89% of the explants, but with only 3.5 SEs per explant, while the explants cultivated on media supplemented with NAA+GA3 or NAA +
GA3 + 2.5 μM PAC regenerated at 100% with 17.7 and 34.6 SEs per explant, respectively. However, in the absence of GA3, higher levels of PAC were needed: 5-10 μM PAC + NAA for 100%-response and 15-22.2 SEs per explant. To get insight into the NAA-GA3-PAC interaction, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and degradation (GA2-oxidase) was analyzed in the explants during 28 days of SE-induction period, using quantitative real-time PCR. In the explants with high embryogenic capacity, the expression levels of SoGA20 ox and SoGA3 ox were significantly lower, and SoGA2-ox1 and SoGA2-ox3 significantly higher than in those with low embryogenic capacity, cultivated on medium with NAA alone, during the whole SE induction period, indicating that NAA-GA3-PAC interaction enabled the acquisition of embryogenic capacity by impacting GA metabolism.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5366
ER  - 
@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2022",
abstract = "Gibberellic acid (GA3) is indispensable for somatic embryo (SE) induction from root apices of spinach. To elucidate its role in SE induction, paclobutrazol (PAC), an inhibitor of gibberellin biosynthesis, was used. Root apices (1 cm) isolated from SE-derived, in vitro-cultivated plants of a previously selected line with high embryogenic capacity were used for experimentation. The explants were cultivated on media containing 20 μM α-naphthaleneacetic acid (NAA) + 0 or 5 μM GA3 + 0, 1, 2.5, 5 or 10 μM PAC. NAA alone induced SE regeneration in 89% of the explants, but with only 3.5 SEs per explant, while the explants cultivated on media supplemented with NAA+GA3 or NAA +
GA3 + 2.5 μM PAC regenerated at 100% with 17.7 and 34.6 SEs per explant, respectively. However, in the absence of GA3, higher levels of PAC were needed: 5-10 μM PAC + NAA for 100%-response and 15-22.2 SEs per explant. To get insight into the NAA-GA3-PAC interaction, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and degradation (GA2-oxidase) was analyzed in the explants during 28 days of SE-induction period, using quantitative real-time PCR. In the explants with high embryogenic capacity, the expression levels of SoGA20 ox and SoGA3 ox were significantly lower, and SoGA2-ox1 and SoGA2-ox3 significantly higher than in those with low embryogenic capacity, cultivated on medium with NAA alone, during the whole SE induction period, indicating that NAA-GA3-PAC interaction enabled the acquisition of embryogenic capacity by impacting GA metabolism.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5366"
}
Belić, M., Zdravković-Korać, S.,& Milojević, J.. (2022). Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 27.
https://hdl.handle.net/21.15107/rcub_ibiss_5366
Belić M, Zdravković-Korać S, Milojević J. Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:27.
https://hdl.handle.net/21.15107/rcub_ibiss_5366 .
Belić, Maja, Zdravković-Korać, Snežana, Milojević, Jelena, "Paclobutrazol and GA3 synergistically promote somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):27,
https://hdl.handle.net/21.15107/rcub_ibiss_5366 .

Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach

Zdravković-Korać, Snežana; Belić, Maja; Ćalić, Dušica; Milojević, Jelena

(Belgrade: Serbian Plant Physiology Society, 2022)

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5331
AB  - Epigenetic modifications of chromatin play a pivotal role in regulation of expression of genes involved in somatic embryo (SE) induction. Hence, the compounds which affect DNA-histone interaction may trigger somatic embryogenesis. Trichostatin (TSA) is a potent inhibitor of histone deacetylases, whose activity leads to increased histone acetylation, thereby affecting gene expression. To explore epigenetic control of SE regeneration from root apices (1 cm) of spinach seedlings, the explants were cultivated on media supplemented with 0, 0.1, 0.5, 1 or 5 μM TSA + 0, 1, 10 or 20 μM α naphthaleneacetic acid (NAA) + 0 or 5 μM gibberellic acid (GA3). The explants were exposed to TSA for 1, 7 or 14 days, and subsequently subcultivated on TSA free medium of the same composition. TSA was dissolved in dimethyl sulfoxide (DMSO), whose final concentration in all media, including TSA-free controls, was 0.05%. TSA was not sufficient to induce SEs either alone or in combination with NAA or GA3. SE regeneration was observed only from the explants cultivated on media supplemented with 10 or 20 μM NAA + 5 μM GA3. In both combinations, TSA promoted somatic embryogenesis, but longer TSA treatment was needed with 10 μM NAA than with 20 μMNAA for efficient SE induction. The highest embryogenic response was attained with 0.1-0.5 μM TSA. DMSO also significantly improved SE induction, probably by enhancing NAA and GA3 intake into the plant cells. The results indicate a significant role of epigenetic control in SE induction in spinach.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5331
ER  - 
@conference{
author = "Zdravković-Korać, Snežana and Belić, Maja and Ćalić, Dušica and Milojević, Jelena",
year = "2022",
abstract = "Epigenetic modifications of chromatin play a pivotal role in regulation of expression of genes involved in somatic embryo (SE) induction. Hence, the compounds which affect DNA-histone interaction may trigger somatic embryogenesis. Trichostatin (TSA) is a potent inhibitor of histone deacetylases, whose activity leads to increased histone acetylation, thereby affecting gene expression. To explore epigenetic control of SE regeneration from root apices (1 cm) of spinach seedlings, the explants were cultivated on media supplemented with 0, 0.1, 0.5, 1 or 5 μM TSA + 0, 1, 10 or 20 μM α naphthaleneacetic acid (NAA) + 0 or 5 μM gibberellic acid (GA3). The explants were exposed to TSA for 1, 7 or 14 days, and subsequently subcultivated on TSA free medium of the same composition. TSA was dissolved in dimethyl sulfoxide (DMSO), whose final concentration in all media, including TSA-free controls, was 0.05%. TSA was not sufficient to induce SEs either alone or in combination with NAA or GA3. SE regeneration was observed only from the explants cultivated on media supplemented with 10 or 20 μM NAA + 5 μM GA3. In both combinations, TSA promoted somatic embryogenesis, but longer TSA treatment was needed with 10 μM NAA than with 20 μMNAA for efficient SE induction. The highest embryogenic response was attained with 0.1-0.5 μM TSA. DMSO also significantly improved SE induction, probably by enhancing NAA and GA3 intake into the plant cells. The results indicate a significant role of epigenetic control in SE induction in spinach.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5331"
}
Zdravković-Korać, S., Belić, M., Ćalić, D.,& Milojević, J.. (2022). Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 25.
https://hdl.handle.net/21.15107/rcub_ibiss_5331
Zdravković-Korać S, Belić M, Ćalić D, Milojević J. Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:25.
https://hdl.handle.net/21.15107/rcub_ibiss_5331 .
Zdravković-Korać, Snežana, Belić, Maja, Ćalić, Dušica, Milojević, Jelena, "Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):25,
https://hdl.handle.net/21.15107/rcub_ibiss_5331 .

Могу ли магнети помоћи биљкама?

Ćalić, Dušica; Ristić Djurović, Jasna; Ćirković, Saša; Milojević, Jelena; Belić, Maja; Zdravković-Korać, Snežana

(Belgrade: Serbian Biological Society, 2022)

TY  - CONF
AU  - Ćalić, Dušica
AU  - Ristić Djurović, Jasna
AU  - Ćirković, Saša
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5152
AB  - Стимулација семена магнетним пољем је биолошки безбедна, јефтина, популарна, еколошки прихватљива и неинвазивна метода са благотворним ефектима на биљке. Примена магнетних поља смањује употребу пестицида, инсектицида и омогућава производњу еколошки безбедне хране (Maffei1), што је у скаду са стратегијом ЕУ “Од њиве до вилице” (“Farm to fork”). Стратегија предвиђа да се до 2030. године смањи употреба пестицида за 50% и обезбеди удео еколошке пољопривредне производње од 25% у укупној пољопривреди. С обзиром на тренутних 0.60% површина под органском производњом у Србији, значајније повећање постаје реално само уз примену иновативних начина гајења пољопривредних култура. У овој студији, магнетна поља различитих јачина (50, 100 и 300 мТ) и времена експозиције показала су позитиван ефекат на клијавост семена купуса, раст клијанаца, дужину и ширину корена, број формираних листова, масу главице, садржај хлорофила, каротеноида и ксантофила, садржај каталаза и пероксидаза, витамина Ц, микро и макро елемената. У листовима третираног купуса детектовано је до 50 % више гвожђа, и два пута мање натријума него у контроли. Принос третираног купуса на огледном пољу земљорадничке задруге „Пољо-флора“ нашег највећег произвођача и извозника киселог купуса је 30 % повећан у односу на контролу. Добијени резултати сугеришу да се магнетна поља могу користити у органској пољопривреди и производњи здраве хране, што је у складу са стратегијом ЕУ.
AB  - Stimulacija semena magnetnim poljem je biološki bezbedna, jeftina, popularna, ekološki prihvatljiva i neinvazivna metoda sa blagotvornim efektima na biljke. Primena magnetnih polja smanjuje upotrebu pesticida, insekticida i omogućava proizvodnju ekološki bezbedne hrane (Maffei1), što je u skadu sa strategijom EU “Od njive do vilice” (“Farm to fork”). Strategija predviđa da se do 2030. godine smanji upotreba pesticida za 50% i obezbedi udeo ekološke poljoprivredne proizvodnje od 25% u ukupnoj poljoprivredi. S obzirom na trenutnih 0.60% površina pod organskom proizvodnjom u Srbiji, značajnije povećanje postaje realno samo uz primenu inovativnih načina gajenja poljoprivrednih kultura. U ovoj studiji, magnetna polja različitih jačina (50, 100 i 300 mT) i vremena ekspozicije pokazala su pozitivan efekat na klijavost semena kupusa, rast klijanaca, dužinu i širinu korena, broj formiranih listova, masu glavice, sadržaj hlorofila, karotenoida i ksantofila, sadržaj katalaza i peroksidaza, vitamina C, mikro i makro elemenata. U listovima tretiranog kupusa detektovano je do 50 % više gvožđa, i dva puta manje natrijuma nego u kontroli. Prinos tretiranog kupusa na oglednom polju zemljoradničke zadruge „Poljo-flora“ našeg najvećeg proizvođača i izvoznika kiselog kupusa je 30 % povećan u odnosu na kontrolu. Dobijeni rezultati sugerišu da se magnetna polja mogu koristiti u organskoj poljoprivredi i proizvodnji zdrave hrane, što je u skladu sa strategijom EU.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Могу ли магнети помоћи биљкама?
T1  - Mogu li magneti pomoći biljkama?
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5152
ER  - 
@conference{
author = "Ćalić, Dušica and Ristić Djurović, Jasna and Ćirković, Saša and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana",
year = "2022",
abstract = "Стимулација семена магнетним пољем је биолошки безбедна, јефтина, популарна, еколошки прихватљива и неинвазивна метода са благотворним ефектима на биљке. Примена магнетних поља смањује употребу пестицида, инсектицида и омогућава производњу еколошки безбедне хране (Maffei1), што је у скаду са стратегијом ЕУ “Од њиве до вилице” (“Farm to fork”). Стратегија предвиђа да се до 2030. године смањи употреба пестицида за 50% и обезбеди удео еколошке пољопривредне производње од 25% у укупној пољопривреди. С обзиром на тренутних 0.60% површина под органском производњом у Србији, значајније повећање постаје реално само уз примену иновативних начина гајења пољопривредних култура. У овој студији, магнетна поља различитих јачина (50, 100 и 300 мТ) и времена експозиције показала су позитиван ефекат на клијавост семена купуса, раст клијанаца, дужину и ширину корена, број формираних листова, масу главице, садржај хлорофила, каротеноида и ксантофила, садржај каталаза и пероксидаза, витамина Ц, микро и макро елемената. У листовима третираног купуса детектовано је до 50 % више гвожђа, и два пута мање натријума него у контроли. Принос третираног купуса на огледном пољу земљорадничке задруге „Пољо-флора“ нашег највећег произвођача и извозника киселог купуса је 30 % повећан у односу на контролу. Добијени резултати сугеришу да се магнетна поља могу користити у органској пољопривреди и производњи здраве хране, што је у складу са стратегијом ЕУ., Stimulacija semena magnetnim poljem je biološki bezbedna, jeftina, popularna, ekološki prihvatljiva i neinvazivna metoda sa blagotvornim efektima na biljke. Primena magnetnih polja smanjuje upotrebu pesticida, insekticida i omogućava proizvodnju ekološki bezbedne hrane (Maffei1), što je u skadu sa strategijom EU “Od njive do vilice” (“Farm to fork”). Strategija predviđa da se do 2030. godine smanji upotreba pesticida za 50% i obezbedi udeo ekološke poljoprivredne proizvodnje od 25% u ukupnoj poljoprivredi. S obzirom na trenutnih 0.60% površina pod organskom proizvodnjom u Srbiji, značajnije povećanje postaje realno samo uz primenu inovativnih načina gajenja poljoprivrednih kultura. U ovoj studiji, magnetna polja različitih jačina (50, 100 i 300 mT) i vremena ekspozicije pokazala su pozitivan efekat na klijavost semena kupusa, rast klijanaca, dužinu i širinu korena, broj formiranih listova, masu glavice, sadržaj hlorofila, karotenoida i ksantofila, sadržaj katalaza i peroksidaza, vitamina C, mikro i makro elemenata. U listovima tretiranog kupusa detektovano je do 50 % više gvožđa, i dva puta manje natrijuma nego u kontroli. Prinos tretiranog kupusa na oglednom polju zemljoradničke zadruge „Poljo-flora“ našeg najvećeg proizvođača i izvoznika kiselog kupusa je 30 % povećan u odnosu na kontrolu. Dobijeni rezultati sugerišu da se magnetna polja mogu koristiti u organskoj poljoprivredi i proizvodnji zdrave hrane, što je u skladu sa strategijom EU.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Могу ли магнети помоћи биљкама?, Mogu li magneti pomoći biljkama?",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5152"
}
Ćalić, D., Ristić Djurović, J., Ćirković, S., Milojević, J., Belić, M.,& Zdravković-Korać, S.. (2022). Могу ли магнети помоћи биљкама?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5152
Ćalić D, Ristić Djurović J, Ćirković S, Milojević J, Belić M, Zdravković-Korać S. Могу ли магнети помоћи биљкама?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5152 .
Ćalić, Dušica, Ristić Djurović, Jasna, Ćirković, Saša, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, "Могу ли магнети помоћи биљкама?" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5152 .

Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)

Belić, Maja; Zdravković-Korać, Snežana; Pavlović, Suzana; Živković, Suzana; Vinterhalter, Dragan; Ćalić, Dušica; Milojević, Jelena

(East Sarajevo : Faculty of Agriculture, 2021)

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5412
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
PB  - eas
C3  - Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5412
ER  - 
@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture, eas",
journal = "Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5412"
}
Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
East Sarajevo : Faculty of Agriculture., 213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412
Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina. 2021;:213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .
Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina (2021):213,
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars

Ćalić, Dušica; Milojević, Jelena; Belić, Maja; Miletić, Rade; Zdravković-Korać, Snežana

(Lausanne: Frontiers Media SA, 2021)

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Miletić, Rade
AU  - Zdravković-Korać, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4319
AB  - Globalization has drastically reduced the number of autochthon apple cultivars in the Serbian market and most of them have nearly disappeared; however, some of these cultivars, such as Petrovača, Budimka, Kolačara Pozna, and Kožara, have extraordinary quality, good pomological characteristics, and pest and disease resistance. The present study was conducted to develop a protocol for the storage of pollen for further use in the conservation and breeding of these cultivars. Viability and germination of the mature pollen were tested in vitro, at four storage temperatures (20, 4, −20, and −80°C), right after harvest or 1, 2, 3, 4, 5, and 6 months after storage. Differences in fresh pollen viability and germination between cultivars were statistically significant and ranged from 60 to 88% and 59 to 98%, respectively. Fresh pollen of cv. Budimka showed the highest viability and germination in comparison with other cultivars, especially cv. Kožara. Pollen viability and germination decreased over the storage period, and it was the lowest after 6 months of storage at room temperature in all tested cultivars. Storage at 4°C prolonged the pollen viability and germinability of 1–5 fold, depending on the cultivar and treatment duration; however, the pollen longevity of all cultivars was significantly extended when stored at −20 or −80°C. After 6 months, pollen of cv. Budimka stored at −20 and −80°C showed 14–15 fold higher germination rates in relation to pollen storage at room temperature for the same period. The results of the present study suggest that the pollen of these apple cultivars could be efficiently maintained at −20°C and could be further used for breeding purposes, e.g., for crossings between cultivars that flower at different times of the year.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars
VL  - 12
DO  - 10.3389/fpls.2021.709231
SP  - 709231
ER  - 
@article{
author = "Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Miletić, Rade and Zdravković-Korać, Snežana",
year = "2021",
abstract = "Globalization has drastically reduced the number of autochthon apple cultivars in the Serbian market and most of them have nearly disappeared; however, some of these cultivars, such as Petrovača, Budimka, Kolačara Pozna, and Kožara, have extraordinary quality, good pomological characteristics, and pest and disease resistance. The present study was conducted to develop a protocol for the storage of pollen for further use in the conservation and breeding of these cultivars. Viability and germination of the mature pollen were tested in vitro, at four storage temperatures (20, 4, −20, and −80°C), right after harvest or 1, 2, 3, 4, 5, and 6 months after storage. Differences in fresh pollen viability and germination between cultivars were statistically significant and ranged from 60 to 88% and 59 to 98%, respectively. Fresh pollen of cv. Budimka showed the highest viability and germination in comparison with other cultivars, especially cv. Kožara. Pollen viability and germination decreased over the storage period, and it was the lowest after 6 months of storage at room temperature in all tested cultivars. Storage at 4°C prolonged the pollen viability and germinability of 1–5 fold, depending on the cultivar and treatment duration; however, the pollen longevity of all cultivars was significantly extended when stored at −20 or −80°C. After 6 months, pollen of cv. Budimka stored at −20 and −80°C showed 14–15 fold higher germination rates in relation to pollen storage at room temperature for the same period. The results of the present study suggest that the pollen of these apple cultivars could be efficiently maintained at −20°C and could be further used for breeding purposes, e.g., for crossings between cultivars that flower at different times of the year.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars",
volume = "12",
doi = "10.3389/fpls.2021.709231",
pages = "709231"
}
Ćalić, D., Milojević, J., Belić, M., Miletić, R.,& Zdravković-Korać, S.. (2021). Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 12, 709231.
https://doi.org/10.3389/fpls.2021.709231
Ćalić D, Milojević J, Belić M, Miletić R, Zdravković-Korać S. Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars. in Frontiers in Plant Science. 2021;12:709231.
doi:10.3389/fpls.2021.709231 .
Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Miletić, Rade, Zdravković-Korać, Snežana, "Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars" in Frontiers in Plant Science, 12 (2021):709231,
https://doi.org/10.3389/fpls.2021.709231 . .
3
12
11

Biotechnological Approach for Cultivation of Allium Species

Zdravković-Korać, Snežana; Belić, Maja; Milojević, Jelena

(Hauppauge, New York : Nova Science Publishers, 2020)

TY  - CHAP
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3949
UR  - https://novapublishers.com/shop/allium-ecology-distribution-and-cultivation/
PB  - Hauppauge, New York : Nova Science Publishers
T2  - Allium: Ecology, Distribution and Cultivation
T1  - Biotechnological Approach for Cultivation of Allium Species
SP  - 187
EP  - 260
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3949
ER  - 
@inbook{
author = "Zdravković-Korać, Snežana and Belić, Maja and Milojević, Jelena",
year = "2020",
publisher = "Hauppauge, New York : Nova Science Publishers",
journal = "Allium: Ecology, Distribution and Cultivation",
booktitle = "Biotechnological Approach for Cultivation of Allium Species",
pages = "187-260",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3949"
}
Zdravković-Korać, S., Belić, M.,& Milojević, J.. (2020). Biotechnological Approach for Cultivation of Allium Species. in Allium: Ecology, Distribution and Cultivation
Hauppauge, New York : Nova Science Publishers., 187-260.
https://hdl.handle.net/21.15107/rcub_ibiss_3949
Zdravković-Korać S, Belić M, Milojević J. Biotechnological Approach for Cultivation of Allium Species. in Allium: Ecology, Distribution and Cultivation. 2020;:187-260.
https://hdl.handle.net/21.15107/rcub_ibiss_3949 .
Zdravković-Korać, Snežana, Belić, Maja, Milojević, Jelena, "Biotechnological Approach for Cultivation of Allium Species" in Allium: Ecology, Distribution and Cultivation (2020):187-260,
https://hdl.handle.net/21.15107/rcub_ibiss_3949 .

Variability in somatic embryo-forming capacity of spinach

Belić, Maja; Zdravković-Korać, Snežana; Uzelac, Branka; Ćalić, Dušica; Pavlović, Suzana; Milojević, Jelena

(Nature Research, 2020)

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Ćalić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3997
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
SP  - 19290
ER  - 
@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Ćalić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9",
pages = "19290"
}
Belić, M., Zdravković-Korać, S., Uzelac, B., Ćalić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research., 10(1), 19290.
https://doi.org/10.1038/s41598-020-76279-9
Belić M, Zdravković-Korać S, Uzelac B, Ćalić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1):19290.
doi:10.1038/s41598-020-76279-9 .
Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Ćalić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020):19290,
https://doi.org/10.1038/s41598-020-76279-9 . .
5
4

Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower

Pavlović, Suzana; Savić, Jelena; Milojević, Jelena; Vinterhalter, Branka; Girek, Zdenka; Adžić, Slađan; Zečević, Bogoljub; Banjac, Nevena

(Springer Science and Business Media LLC, 2020)

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Savić, Jelena
AU  - Milojević, Jelena
AU  - Vinterhalter, Branka
AU  - Girek, Zdenka
AU  - Adžić, Slađan
AU  - Zečević, Bogoljub
AU  - Banjac, Nevena
PY  - 2020
UR  - https://doi.org/10.1007/s11240-020-01948-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4011
AB  - Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.
PB  - Springer Science and Business Media LLC
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
IS  - 3
VL  - 143
DO  - 10.1007/s11240-020-01948-6
SP  - 635
EP  - 651
ER  - 
@article{
author = "Pavlović, Suzana and Savić, Jelena and Milojević, Jelena and Vinterhalter, Branka and Girek, Zdenka and Adžić, Slađan and Zečević, Bogoljub and Banjac, Nevena",
year = "2020",
abstract = "Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops. Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules that play important roles in regulating innate immune responses in plants. Based on involvement of tobacco MAP kinase kinase kinase (NPK1) in stress response, the effect of the expression of NPK1 transgene to NaCl salt stress tolerance in cauliflower KFRM4 lines was studied. The Agrobacterium tumefaciens-mediated transformation protocol, using EHA101(pSHX004) vector harbouring the NPK1 and phosphinothricin N-acetyltransferase (bar) genes, the cyclic somatic embryogenesis regeneration pathway, the application of acetosyringone (AS) during co-cultivation and a delayed phosphinothricine (PPT) selection procedure provided sufficient transformation efficiency of 7.33% without escapes. PCR analysis indicated the integration of both NPK1 and bar transgenes in regenerated cauliflower lines. Transgenic cauliflower lines, exposed to NaCl stress in vitro, showed higher growth rates, greater ability to retain chlorophyll and carotenoids, and increased osmotic regulation capacity compared with non-transformed control plants. The tolerance level of transformed lines correlated with the level of NPK1 gene expression estimated by RT-qPCR, and the L2 line with the highest NPK1 expression displayed the greatest tolerance to NaCl stress. None of the obtained cauliflower transformed lines grown in greenhouses showed any morphological or yield differences compared with non-transformed plants. Furthermore, the expression of the bar gene facilitated the tolerance of transformed lines to the total herbicide PPT, applied at concentrations 2-3 times higher than those routinely used for weed control in the crop field. Key message The results underlined that constitutively expressing NPK1 can significantly contribute to enhanced salt stresstolerance in cauliflower, suggesting that this could be a promising basis for the creation of new stress tolerancecruciferous vegetable lines.",
publisher = "Springer Science and Business Media LLC",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower",
number = "3",
volume = "143",
doi = "10.1007/s11240-020-01948-6",
pages = "635-651"
}
Pavlović, S., Savić, J., Milojević, J., Vinterhalter, B., Girek, Z., Adžić, S., Zečević, B.,& Banjac, N.. (2020). Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC)
Springer Science and Business Media LLC., 143(3), 635-651.
https://doi.org/10.1007/s11240-020-01948-6
Pavlović S, Savić J, Milojević J, Vinterhalter B, Girek Z, Adžić S, Zečević B, Banjac N. Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower. in Plant Cell, Tissue and Organ Culture (PCTOC). 2020;143(3):635-651.
doi:10.1007/s11240-020-01948-6 .
Pavlović, Suzana, Savić, Jelena, Milojević, Jelena, Vinterhalter, Branka, Girek, Zdenka, Adžić, Slađan, Zečević, Bogoljub, Banjac, Nevena, "Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower" in Plant Cell, Tissue and Organ Culture (PCTOC), 143, no. 3 (2020):635-651,
https://doi.org/10.1007/s11240-020-01948-6 . .
6
1
5

Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach

Belić, Maja; Zdravković-Korać, Snežana; Janošević, Dušica; Savić, Jelena; Todorović, Slađana; Banjac, Nevena; Milojević, Jelena

(Springer, 2020)

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Janošević, Dušica
AU  - Savić, Jelena
AU  - Todorović, Slađana
AU  - Banjac, Nevena
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3774
AB  - Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach
VL  - 142
DO  - 10.1007/s11240-020-01878-3
SP  - 537
EP  - 548
ER  - 
@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Janošević, Dušica and Savić, Jelena and Todorović, Slađana and Banjac, Nevena and Milojević, Jelena",
year = "2020",
abstract = "Gibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 µM α-naphthaleneacetic acid (NAA) and 0–10 µM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1 and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1 and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expression levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach",
volume = "142",
doi = "10.1007/s11240-020-01878-3",
pages = "537-548"
}
Belić, M., Zdravković-Korać, S., Janošević, D., Savić, J., Todorović, S., Banjac, N.,& Milojević, J.. (2020). Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture
Springer., 142, 537-548.
https://doi.org/10.1007/s11240-020-01878-3
Belić M, Zdravković-Korać S, Janošević D, Savić J, Todorović S, Banjac N, Milojević J. Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach. in Plant Cell, Tissue and Organ Culture. 2020;142:537-548.
doi:10.1007/s11240-020-01878-3 .
Belić, Maja, Zdravković-Korać, Snežana, Janošević, Dušica, Savić, Jelena, Todorović, Slađana, Banjac, Nevena, Milojević, Jelena, "Gibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinach" in Plant Cell, Tissue and Organ Culture, 142 (2020):537-548,
https://doi.org/10.1007/s11240-020-01878-3 . .
5
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7

Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction

Belić, Maja; Zdravković-Korać, Snežana; Milojević, Jelena

(Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences, 2019)

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5592
AB  - Gibberellins (GA) play a pivotal role in the induction of somatic embryogenesis (SE) from the apical root sections of spinach seedlings. To study the role of GA in this process, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-ox and GA3-ox) and degradation (GA2-ox1, -ox2 and -ox3) was analysed in the explants cultivated on inductive medium (IM - supplemented with 20 μM NAA + 5 μM GA3), noninductive medium (NM - containing 20 μM NAA), or plant growth regulator (PGR)-free medium. Expression of these genes was monitored by means of quantitative real-time PCR during 28 days SE-induction period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a control. In the IM-cultivated explants, expression of both GA20-ox and GA3-ox decreased over the whole induction period. On the other hand, in the explants cultivated on NM or PGR-free medium expression of these genes began to increase after 1 or 14 days, respectively, and remained higher than in the control steadily over time. Concerning the catabolic GA2-ox genes, particularly GA2-ox2, their expression was higher in the IM-cultivated explants over the whole SE-induction period comparing to the control. However, in the NM-cultivated explants, expression of GA2-ox2 and GA2-ox3 was mostly lower. The results indicate that continuous expression level decrease in GA20-ox and GA3-ox and increase in GA2-ox2 favor SE regeneration from root-tips of spinach. Understanding the role of GA in SE induction in spinach would not only provide a deeper insight into the mechanism of this process, but also enhance the improvement of this species through biotechnological approach.
PB  - Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences
C3  - Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic
T1  - Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5592
ER  - 
@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2019",
abstract = "Gibberellins (GA) play a pivotal role in the induction of somatic embryogenesis (SE) from the apical root sections of spinach seedlings. To study the role of GA in this process, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-ox and GA3-ox) and degradation (GA2-ox1, -ox2 and -ox3) was analysed in the explants cultivated on inductive medium (IM - supplemented with 20 μM NAA + 5 μM GA3), noninductive medium (NM - containing 20 μM NAA), or plant growth regulator (PGR)-free medium. Expression of these genes was monitored by means of quantitative real-time PCR during 28 days SE-induction period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a control. In the IM-cultivated explants, expression of both GA20-ox and GA3-ox decreased over the whole induction period. On the other hand, in the explants cultivated on NM or PGR-free medium expression of these genes began to increase after 1 or 14 days, respectively, and remained higher than in the control steadily over time. Concerning the catabolic GA2-ox genes, particularly GA2-ox2, their expression was higher in the IM-cultivated explants over the whole SE-induction period comparing to the control. However, in the NM-cultivated explants, expression of GA2-ox2 and GA2-ox3 was mostly lower. The results indicate that continuous expression level decrease in GA20-ox and GA3-ox and increase in GA2-ox2 favor SE regeneration from root-tips of spinach. Understanding the role of GA in SE induction in spinach would not only provide a deeper insight into the mechanism of this process, but also enhance the improvement of this species through biotechnological approach.",
publisher = "Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences",
journal = "Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic",
title = "Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5592"
}
Belić, M., Zdravković-Korać, S.,& Milojević, J.. (2019). Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction. in Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic
Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_5592
Belić M, Zdravković-Korać S, Milojević J. Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction. in Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic. 2019;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_5592 .
Belić, Maja, Zdravković-Korać, Snežana, Milojević, Jelena, "Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction" in Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic (2019):39,
https://hdl.handle.net/21.15107/rcub_ibiss_5592 .

Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus

Zdravković-Korać, Snežana; Tubić, Ljiljana; Devrnja, Nina; Ćalić, Dušica; Milojević, Jelena; Belić, Maja; Savić, Jelena

(2019)

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Savić, Jelena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0304423818308938?via%3Dihub
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0304423818308938
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3224
AB  - Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.
T2  - Scientia Horticulturae
T1  - Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus
VL  - 247
DO  - 10.1016/j.scienta.2018.12.021
SP  - 362
EP  - 372
ER  - 
@article{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Devrnja, Nina and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Savić, Jelena",
year = "2019",
abstract = "Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.",
journal = "Scientia Horticulturae",
title = "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus",
volume = "247",
doi = "10.1016/j.scienta.2018.12.021",
pages = "362-372"
}
Zdravković-Korać, S., Tubić, L., Devrnja, N., Ćalić, D., Milojević, J., Belić, M.,& Savić, J.. (2019). Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae, 247, 362-372.
https://doi.org/10.1016/j.scienta.2018.12.021
Zdravković-Korać S, Tubić L, Devrnja N, Ćalić D, Milojević J, Belić M, Savić J. Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae. 2019;247:362-372.
doi:10.1016/j.scienta.2018.12.021 .
Zdravković-Korać, Snežana, Tubić, Ljiljana, Devrnja, Nina, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Savić, Jelena, "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus" in Scientia Horticulturae, 247 (2019):362-372,
https://doi.org/10.1016/j.scienta.2018.12.021 . .
4
4
6

Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)

Banjac, Nevena; Vinterhalter, Branka; Krstić Milošević, Dijana; Milojević, Jelena; Tubić, Ljiljana; Ghalawenji, Nabil; Zdravković-Korać, Snežana

(2019)

TY  - JOUR
AU  - Banjac, Nevena
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Ghalawenji, Nabil
AU  - Zdravković-Korać, Snežana
PY  - 2019
UR  - http://link.springer.com/10.1007/s11240-019-01595-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3310
AB  - Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)
DO  - 10.1007/s11240-019-01595-6
ER  - 
@article{
author = "Banjac, Nevena and Vinterhalter, Branka and Krstić Milošević, Dijana and Milojević, Jelena and Tubić, Ljiljana and Ghalawenji, Nabil and Zdravković-Korać, Snežana",
year = "2019",
abstract = "Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)",
doi = "10.1007/s11240-019-01595-6"
}
Banjac, N., Vinterhalter, B., Krstić Milošević, D., Milojević, J., Tubić, L., Ghalawenji, N.,& Zdravković-Korać, S.. (2019). Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC).
https://doi.org/10.1007/s11240-019-01595-6
Banjac N, Vinterhalter B, Krstić Milošević D, Milojević J, Tubić L, Ghalawenji N, Zdravković-Korać S. Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC). 2019;.
doi:10.1007/s11240-019-01595-6 .
Banjac, Nevena, Vinterhalter, Branka, Krstić Milošević, Dijana, Milojević, Jelena, Tubić, Ljiljana, Ghalawenji, Nabil, Zdravković-Korać, Snežana, "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)" in Plant Cell, Tissue and Organ Culture (PCTOC) (2019),
https://doi.org/10.1007/s11240-019-01595-6 . .
4
5
1
6

Influence of bio- and magneto-priming on flax seedlings growth

Ćalić, Dušica; Devrnja, Nina; Milojević, Jelena; Belić, Maja; Zdravković-Korać, Snežana; Karličić, Vera; Raičević, Vera; Ćirković, Saša; Jasna, Ristić-Đurović

(Belgrade: Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Karličić, Vera
AU  - Raičević, Vera
AU  - Ćirković, Saša
AU  - Jasna, Ristić-Đurović
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4330
AB  - Flax (Linum usitatissimum) is an important food and fiber crop, due to its nutritional and nutraceutical qualities. Flax seeds have been consumed for at least 6,000 years, which makes it one of
the world’s first cultivated crops. Seed priming with living bacterial inoculum, termed biopriming,
involves the application of plant growth-promoting rhizobacteria. The present study was conducted to investigate the effect of growth-promoting rhizobacterium Bacillus amyloliquefaciens
on the growth of flax seedlings under different strengths of magnetic field. Seedlings were soaked
in bacterial suspension and bacteria were allowed to colonize. After ten minutes of soaking, the
seedlings were exposed to magnetic field of 60 and 90 mT for 24 h, in dark under laboratory conditions. Untreated seedlings were used as controls. Shoot and root length, number and length of
newly formed lateral roots and biomass production were measured after 1, 3, 5, 7 and 10 days. Results indicate that the combinations of magnetic field and plant growth-promoting bacteria gave
half- to two-fold better results than bacteria or magnetic field alone. The best result was achieved
in seedlings treated with Bacillus amyloliquefaciens and 90 mT magnetic field. This work showed a
significant impact of bio- and magneto-priming on the improvement of flax seedling growth and
biomass productivity.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Influence of bio- and magneto-priming on flax seedlings growth
SP  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4330
ER  - 
@conference{
author = "Ćalić, Dušica and Devrnja, Nina and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana and Karličić, Vera and Raičević, Vera and Ćirković, Saša and Jasna, Ristić-Đurović",
year = "2018",
abstract = "Flax (Linum usitatissimum) is an important food and fiber crop, due to its nutritional and nutraceutical qualities. Flax seeds have been consumed for at least 6,000 years, which makes it one of
the world’s first cultivated crops. Seed priming with living bacterial inoculum, termed biopriming,
involves the application of plant growth-promoting rhizobacteria. The present study was conducted to investigate the effect of growth-promoting rhizobacterium Bacillus amyloliquefaciens
on the growth of flax seedlings under different strengths of magnetic field. Seedlings were soaked
in bacterial suspension and bacteria were allowed to colonize. After ten minutes of soaking, the
seedlings were exposed to magnetic field of 60 and 90 mT for 24 h, in dark under laboratory conditions. Untreated seedlings were used as controls. Shoot and root length, number and length of
newly formed lateral roots and biomass production were measured after 1, 3, 5, 7 and 10 days. Results indicate that the combinations of magnetic field and plant growth-promoting bacteria gave
half- to two-fold better results than bacteria or magnetic field alone. The best result was achieved
in seedlings treated with Bacillus amyloliquefaciens and 90 mT magnetic field. This work showed a
significant impact of bio- and magneto-priming on the improvement of flax seedling growth and
biomass productivity.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Influence of bio- and magneto-priming on flax seedlings growth",
pages = "140-141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4330"
}
Ćalić, D., Devrnja, N., Milojević, J., Belić, M., Zdravković-Korać, S., Karličić, V., Raičević, V., Ćirković, S.,& Jasna, R.. (2018). Influence of bio- and magneto-priming on flax seedlings growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 140-141.
https://hdl.handle.net/21.15107/rcub_ibiss_4330
Ćalić D, Devrnja N, Milojević J, Belić M, Zdravković-Korać S, Karličić V, Raičević V, Ćirković S, Jasna R. Influence of bio- and magneto-priming on flax seedlings growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:140-141.
https://hdl.handle.net/21.15107/rcub_ibiss_4330 .
Ćalić, Dušica, Devrnja, Nina, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, Karličić, Vera, Raičević, Vera, Ćirković, Saša, Jasna, Ristić-Đurović, "Influence of bio- and magneto-priming on flax seedlings growth" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):140-141,
https://hdl.handle.net/21.15107/rcub_ibiss_4330 .

Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration

Zdravković-Korać, Snežana; Ćalić, Dušica; Devrnja, Nina; Belić, Maja; Milojević, Jelena

(Belgrade: Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Belić, Maja
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4327
AB  - Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4327
ER  - 
@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Devrnja, Nina and Belić, Maja and Milojević, Jelena",
year = "2018",
abstract = "Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4327"
}
Zdravković-Korać, S., Ćalić, D., Devrnja, N., Belić, M.,& Milojević, J.. (2018). Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327
Zdravković-Korać S, Ćalić D, Devrnja N, Belić M, Milojević J. Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .
Zdravković-Korać, Snežana, Ćalić, Dušica, Devrnja, Nina, Belić, Maja, Milojević, Jelena, "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):38,
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .

Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.

Belić, Maja; Anačkov, Goran; Ćalić, Dušica; Devrnja, Nina; Igić, Ružica; Milojević, Jelena; Zdravković-Korać, Snežana

(Belgrade: Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Belić, Maja
AU  - Anačkov, Goran
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Igić, Ružica
AU  - Milojević, Jelena
AU  - Zdravković-Korać, Snežana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4325
AB  - A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4325
ER  - 
@conference{
author = "Belić, Maja and Anačkov, Goran and Ćalić, Dušica and Devrnja, Nina and Igić, Ružica and Milojević, Jelena and Zdravković-Korać, Snežana",
year = "2018",
abstract = "A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4325"
}
Belić, M., Anačkov, G., Ćalić, D., Devrnja, N., Igić, R., Milojević, J.,& Zdravković-Korać, S.. (2018). Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325
Belić M, Anačkov G, Ćalić D, Devrnja N, Igić R, Milojević J, Zdravković-Korać S. Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .
Belić, Maja, Anačkov, Goran, Ćalić, Dušica, Devrnja, Nina, Igić, Ružica, Milojević, Jelena, Zdravković-Korać, Snežana, "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit." in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):30,
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .

Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections

Belić, Maja; Devrnja, Nina; Ćalić, Dušica; Zdravković-Korać, Snežana; Milojević, Jelena

(Belgrade: Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Belić, Maja
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4324
AB  - Gibberellins (GA) are essential for induction of somatic embryogenesis from spinach apical root
sections. To elucidate the role of GA in this process, expression of genes encoding enzymes that
catalyze the final step of GA20 oxidation to bioactive GA, GA3-oxidase (GA3-ox), and GA degradation, GA2-oxidase (GA2-ox), was assessed. In spinach, there is only one GA3-ox and three GA2-ox
(GA2ox 1, GA2 ox2, GA2 ox3). Expression of these genes was tested in the explants cultivated on
noninductive medium (NM), supplemented with 20 μM NAA, inductive medium (IM), containing
20 μM NAA + 5 μM GA3, or plant growth regulator (PGR)-free medium during a 28 d induction
period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a
control. In the explants cultivated on PGR-free medium, expression of GA3-ox increased gradually
up to 10-fold and was constantly higher than in control. GA2-ox1, with an increase of up to 60-fold, was the most highly expressed GA2-ox, while the expression of GA2-ox2 and GA2-ox3 only
slightly increased compared to the control. In the explants cultivated on NM, expression of GA3-ox
decreased slightly until the 7th day of cultivation and then increased up to 2-fold until the end
of the experiment, while the expression of GA2-ox1 and GA2-ox2 was only slightly higher than
in control. However, explants cultivated on IM showed the constant and significant decrease of
GA3-ox (down to 7-fold) and increase of GA2-ox2 (up to 20-fold) expression. Here, we propose that
continuous decrease in GA3-ox and increase in GA2-ox2 expression were favorable conditions for
somatic embryo induction.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections
SP  - 29
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4324
ER  - 
@conference{
author = "Belić, Maja and Devrnja, Nina and Ćalić, Dušica and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2018",
abstract = "Gibberellins (GA) are essential for induction of somatic embryogenesis from spinach apical root
sections. To elucidate the role of GA in this process, expression of genes encoding enzymes that
catalyze the final step of GA20 oxidation to bioactive GA, GA3-oxidase (GA3-ox), and GA degradation, GA2-oxidase (GA2-ox), was assessed. In spinach, there is only one GA3-ox and three GA2-ox
(GA2ox 1, GA2 ox2, GA2 ox3). Expression of these genes was tested in the explants cultivated on
noninductive medium (NM), supplemented with 20 μM NAA, inductive medium (IM), containing
20 μM NAA + 5 μM GA3, or plant growth regulator (PGR)-free medium during a 28 d induction
period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a
control. In the explants cultivated on PGR-free medium, expression of GA3-ox increased gradually
up to 10-fold and was constantly higher than in control. GA2-ox1, with an increase of up to 60-fold, was the most highly expressed GA2-ox, while the expression of GA2-ox2 and GA2-ox3 only
slightly increased compared to the control. In the explants cultivated on NM, expression of GA3-ox
decreased slightly until the 7th day of cultivation and then increased up to 2-fold until the end
of the experiment, while the expression of GA2-ox1 and GA2-ox2 was only slightly higher than
in control. However, explants cultivated on IM showed the constant and significant decrease of
GA3-ox (down to 7-fold) and increase of GA2-ox2 (up to 20-fold) expression. Here, we propose that
continuous decrease in GA3-ox and increase in GA2-ox2 expression were favorable conditions for
somatic embryo induction.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections",
pages = "29-30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4324"
}
Belić, M., Devrnja, N., Ćalić, D., Zdravković-Korać, S.,& Milojević, J.. (2018). Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 29-30.
https://hdl.handle.net/21.15107/rcub_ibiss_4324
Belić M, Devrnja N, Ćalić D, Zdravković-Korać S, Milojević J. Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:29-30.
https://hdl.handle.net/21.15107/rcub_ibiss_4324 .
Belić, Maja, Devrnja, Nina, Ćalić, Dušica, Zdravković-Korać, Snežana, Milojević, Jelena, "Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):29-30,
https://hdl.handle.net/21.15107/rcub_ibiss_4324 .

Potassium iodide promotes bud regeneration from the apical root sections of shallot plants

Zdravković-Korać, Snežana; Belić, Maja; Devrnja, Nina; Ćalić, Dušica; Milojević, Jelena

(Belgrade: Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4322
AB  - The apical root sections of Allium ascalonicum plants respond to a callus inducing treatment by
forming callus from the root apical meristem. It has been shown in Arabidopsis thaliana that balance between superoxide and hydrogen peroxide (H2O2) in the root tip determines root meristem
size by controlling the transition from cell proliferation to differentiation. Hence, the increased size
of root meristem may affect callus formation and subsequent bud regeneration from the root-tips.
To test this hypothesis, in vitro-grown shallot plants were pretreated with aqueous solutions of
H2O2 (100, 500 or 1000 μM) or potassium iodide (KI, 1 or 10 mM) for 24h. Plants incubated in water
were used as a control. The apical root sections (1 cm) isolated from these plants were cultivated
on callus induction medium, supplemented with 5 μM 2,4-D + 5 μM BA, for 8 weeks and then on
regeneration induction medium, containing 5 μM BA, for 4 weeks. Obtained results indicate the
significant impact of KI on de novo bud regeneration. Explants treated with 1 mM KI regenerated
buds with significantly higher frequency (83.6% vs. 25.2%) and the mean bud number (6.19 vs.
0.21) compared to the control. The frequencies of bud regeneration and the mean bud numbers obtained for H2O2-treated explants were significantly lower compared to KI-treated explants
(43.5% 52.0% and 1.0-2.3, respectively). Assuming that H2O2 decreases size of the root meristem,
as was shown in Arabidopsis, the results obtained in this work suggest that KI, a H2O2 scavenger,
increased bud-forming capacity presumably by increasing the size of root meristem.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Potassium iodide promotes bud regeneration from the apical root sections of shallot plants
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4322
ER  - 
@conference{
author = "Zdravković-Korać, Snežana and Belić, Maja and Devrnja, Nina and Ćalić, Dušica and Milojević, Jelena",
year = "2018",
abstract = "The apical root sections of Allium ascalonicum plants respond to a callus inducing treatment by
forming callus from the root apical meristem. It has been shown in Arabidopsis thaliana that balance between superoxide and hydrogen peroxide (H2O2) in the root tip determines root meristem
size by controlling the transition from cell proliferation to differentiation. Hence, the increased size
of root meristem may affect callus formation and subsequent bud regeneration from the root-tips.
To test this hypothesis, in vitro-grown shallot plants were pretreated with aqueous solutions of
H2O2 (100, 500 or 1000 μM) or potassium iodide (KI, 1 or 10 mM) for 24h. Plants incubated in water
were used as a control. The apical root sections (1 cm) isolated from these plants were cultivated
on callus induction medium, supplemented with 5 μM 2,4-D + 5 μM BA, for 8 weeks and then on
regeneration induction medium, containing 5 μM BA, for 4 weeks. Obtained results indicate the
significant impact of KI on de novo bud regeneration. Explants treated with 1 mM KI regenerated
buds with significantly higher frequency (83.6% vs. 25.2%) and the mean bud number (6.19 vs.
0.21) compared to the control. The frequencies of bud regeneration and the mean bud numbers obtained for H2O2-treated explants were significantly lower compared to KI-treated explants
(43.5% 52.0% and 1.0-2.3, respectively). Assuming that H2O2 decreases size of the root meristem,
as was shown in Arabidopsis, the results obtained in this work suggest that KI, a H2O2 scavenger,
increased bud-forming capacity presumably by increasing the size of root meristem.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Potassium iodide promotes bud regeneration from the apical root sections of shallot plants",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4322"
}
Zdravković-Korać, S., Belić, M., Devrnja, N., Ćalić, D.,& Milojević, J.. (2018). Potassium iodide promotes bud regeneration from the apical root sections of shallot plants. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_4322
Zdravković-Korać S, Belić M, Devrnja N, Ćalić D, Milojević J. Potassium iodide promotes bud regeneration from the apical root sections of shallot plants. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_4322 .
Zdravković-Korać, Snežana, Belić, Maja, Devrnja, Nina, Ćalić, Dušica, Milojević, Jelena, "Potassium iodide promotes bud regeneration from the apical root sections of shallot plants" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):39,
https://hdl.handle.net/21.15107/rcub_ibiss_4322 .

Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth

Ćalić, Dušica; Todorović, Dajana; Petković, Branka; Devrnja, Nina; Milojević, Jelena; Belić, Maja; Zdravković-Korać, Snežana

(Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Ćalić, Dušica
AU  - Todorović, Dajana
AU  - Petković, Branka
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4015
AB  - In the context of seed technology, the use of magneto-priming, as a method for increasing
plant production, offers advantages in comparison to conventional treatments with chemical substances.
Magneto-priming is a cheap, non-invasive technique, based on the application of magnetic
fields (MF) and described as eco-friendly, with proved beneficial effects on seed germination
and subsequent plant biomass yield. In this study, seeds of flax (Linum usitatissimum), placed on
moist filter-paper, were treated with 100 mT MF strength, with exposure time of 0, 24, 48, 72, 96
and 120 h and kept in dark under laboratory conditions. Seeds that were not exposed to magnetic
field were used as controls. Efficacy of MF on seed germination and seedlings height, shoot and
root length and biomass was evaluated. The MF promoted seed germination rate by 10%-40 %,
depending of treatments, compared to control. The growth parameters were better in seedlings
exposed to all magnetic treatments, with an increase of up to 60% over the control. MF treatment
of 24 h had a 50% better effect than 120 h MF treatment. In addition, the best biomass yield of
acclimated plants was achieved after the shortest magnetic exposure treatment. This study suggests
a positive effect of stable MF on flax seed germination and subsequent growth of seedlings.
Therefore, magnetic field may be useful in organic agriculture in replacing the usage of plant
growth regulators.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković”, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4015
ER  - 
@conference{
author = "Ćalić, Dušica and Todorović, Dajana and Petković, Branka and Devrnja, Nina and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana",
year = "2018",
abstract = "In the context of seed technology, the use of magneto-priming, as a method for increasing
plant production, offers advantages in comparison to conventional treatments with chemical substances.
Magneto-priming is a cheap, non-invasive technique, based on the application of magnetic
fields (MF) and described as eco-friendly, with proved beneficial effects on seed germination
and subsequent plant biomass yield. In this study, seeds of flax (Linum usitatissimum), placed on
moist filter-paper, were treated with 100 mT MF strength, with exposure time of 0, 24, 48, 72, 96
and 120 h and kept in dark under laboratory conditions. Seeds that were not exposed to magnetic
field were used as controls. Efficacy of MF on seed germination and seedlings height, shoot and
root length and biomass was evaluated. The MF promoted seed germination rate by 10%-40 %,
depending of treatments, compared to control. The growth parameters were better in seedlings
exposed to all magnetic treatments, with an increase of up to 60% over the control. MF treatment
of 24 h had a 50% better effect than 120 h MF treatment. In addition, the best biomass yield of
acclimated plants was achieved after the shortest magnetic exposure treatment. This study suggests
a positive effect of stable MF on flax seed germination and subsequent growth of seedlings.
Therefore, magnetic field may be useful in organic agriculture in replacing the usage of plant
growth regulators.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4015"
}
Ćalić, D., Todorović, D., Petković, B., Devrnja, N., Milojević, J., Belić, M.,& Zdravković-Korać, S.. (2018). Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Serbian Plant Physiology Society., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_4015
Ćalić D, Todorović D, Petković B, Devrnja N, Milojević J, Belić M, Zdravković-Korać S. Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_4015 .
Ćalić, Dušica, Todorović, Dajana, Petković, Branka, Devrnja, Nina, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, "Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):141,
https://hdl.handle.net/21.15107/rcub_ibiss_4015 .

Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis

Devrnja, Nina; Janošević, Dušica; Tešević, Vele; Savić, Jelena; Zdravković-Korać, Snežana; Milojević, Jelena; Ćalić, Dušica

(Belgrade: Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Devrnja, Nina
AU  - Janošević, Dušica
AU  - Tešević, Vele
AU  - Savić, Jelena
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
AU  - Ćalić, Dušica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4323
AB  - The morphology and structure of tansy glandular trichomes were characterized for the first
time using scanning electron microscopy and light microscopy, and their secretion was analyzed
by histochemical staining. Leaf indumentum of tansy was covered with non-glandular and glandular trichomes of biseriate type, distributed on both adaxial and abaxial surface. Both types of
trichomes were also distributed along the stem, but with lower density. Light microscopy showed
that biseriate glandular trichomes consisted of two broad basal cells embedded in the epidermis,
a short single-celled stalk, and a large round multicellular head. The histochemical analysis of the
glandular secretion, performed through several staining methods, revealed the complex nature of
the secreted products, including lipids, terpenes, alkaloids and polysaccharides.
GC/MS phytochemical analysis of volatile compounds secreted by glandular trichomes, identified 42 compounds, representing >97% of the total volatiles in EO, obtained by hydrodistillation
in a Clevenger-type apparatus. The monoterpene fraction was dominant, with the prevalence of
oxygenated monoterpenes (38.5%), while the majority of sesquiterpene fraction referred to oxygenated sesquiterpenes (22.6%). Two dominant compounds, which determined the essential oil
chemotype, were trans-thujone (22.7%) and neryl-isovalerate (20.6%).
The essential oil protects the aerial parts of the plant against herbivores and pathogens and the
biological activity of the secreted products indicates their potential as pesticides or pharmaceuticals.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4323
ER  - 
@conference{
author = "Devrnja, Nina and Janošević, Dušica and Tešević, Vele and Savić, Jelena and Zdravković-Korać, Snežana and Milojević, Jelena and Ćalić, Dušica",
year = "2018",
abstract = "The morphology and structure of tansy glandular trichomes were characterized for the first
time using scanning electron microscopy and light microscopy, and their secretion was analyzed
by histochemical staining. Leaf indumentum of tansy was covered with non-glandular and glandular trichomes of biseriate type, distributed on both adaxial and abaxial surface. Both types of
trichomes were also distributed along the stem, but with lower density. Light microscopy showed
that biseriate glandular trichomes consisted of two broad basal cells embedded in the epidermis,
a short single-celled stalk, and a large round multicellular head. The histochemical analysis of the
glandular secretion, performed through several staining methods, revealed the complex nature of
the secreted products, including lipids, terpenes, alkaloids and polysaccharides.
GC/MS phytochemical analysis of volatile compounds secreted by glandular trichomes, identified 42 compounds, representing >97% of the total volatiles in EO, obtained by hydrodistillation
in a Clevenger-type apparatus. The monoterpene fraction was dominant, with the prevalence of
oxygenated monoterpenes (38.5%), while the majority of sesquiterpene fraction referred to oxygenated sesquiterpenes (22.6%). Two dominant compounds, which determined the essential oil
chemotype, were trans-thujone (22.7%) and neryl-isovalerate (20.6%).
The essential oil protects the aerial parts of the plant against herbivores and pathogens and the
biological activity of the secreted products indicates their potential as pesticides or pharmaceuticals.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4323"
}
Devrnja, N., Janošević, D., Tešević, V., Savić, J., Zdravković-Korać, S., Milojević, J.,& Ćalić, D.. (2018). Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 36.
https://hdl.handle.net/21.15107/rcub_ibiss_4323
Devrnja N, Janošević D, Tešević V, Savić J, Zdravković-Korać S, Milojević J, Ćalić D. Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:36.
https://hdl.handle.net/21.15107/rcub_ibiss_4323 .
Devrnja, Nina, Janošević, Dušica, Tešević, Vele, Savić, Jelena, Zdravković-Korać, Snežana, Milojević, Jelena, Ćalić, Dušica, "Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):36,
https://hdl.handle.net/21.15107/rcub_ibiss_4323 .