TY  - JOUR
AU  - Fernandes, Ângela
AU  - Chaski, Christina
AU  - Pereira, Carla
AU  - Kostić, Marina
AU  - Rouphael, Youssef
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Petropoulos, Spyridon A.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/645
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5090
AB  - The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070645
SP  - 645
ER  - 

@article{
author = "Fernandes, Ângela and Chaski, Christina and Pereira, Carla and Kostić, Marina and Rouphael, Youssef and Soković, Marina and Barros, Lillian and Petropoulos, Spyridon A.",
year = "2022",
abstract = "The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070645",
pages = "645"
}

Fernandes, Â., Chaski, C., Pereira, C., Kostić, M., Rouphael, Y., Soković, M., Barros, L.,& Petropoulos, S. A.. (2022). Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae
Basel: MDPI., 8(7), 645.
https://doi.org/10.3390/horticulturae8070645

Fernandes Â, Chaski C, Pereira C, Kostić M, Rouphael Y, Soković M, Barros L, Petropoulos SA. Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae. 2022;8(7):645.
doi:10.3390/horticulturae8070645 .

Fernandes, Ângela, Chaski, Christina, Pereira, Carla, Kostić, Marina, Rouphael, Youssef, Soković, Marina, Barros, Lillian, Petropoulos, Spyridon A., "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit" in Horticulturae, 8, no. 7 (2022):645,
https://doi.org/10.3390/horticulturae8070645 . .

TY  - JOUR
AU  - Carocho, Márcio
AU  - Barros, Lillian
AU  - Morales, Patricia
AU  - Petropoulos, Spyridon A.
AU  - Soković, Marina
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.1077985/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5339
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Editorial: The chemistry of food in the advent of sustainable diets
VL  - 9
DO  - 10.3389/fnut.2022.1077985
SP  - 1077985
ER  - 

@article{
author = "Carocho, Márcio and Barros, Lillian and Morales, Patricia and Petropoulos, Spyridon A. and Soković, Marina",
year = "2022",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Editorial: The chemistry of food in the advent of sustainable diets",
volume = "9",
doi = "10.3389/fnut.2022.1077985",
pages = "1077985"
}

Carocho, M., Barros, L., Morales, P., Petropoulos, S. A.,& Soković, M.. (2022). Editorial: The chemistry of food in the advent of sustainable diets. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 1077985.
https://doi.org/10.3389/fnut.2022.1077985

Carocho M, Barros L, Morales P, Petropoulos SA, Soković M. Editorial: The chemistry of food in the advent of sustainable diets. in Frontiers in Nutrition. 2022;9:1077985.
doi:10.3389/fnut.2022.1077985 .

Carocho, Márcio, Barros, Lillian, Morales, Patricia, Petropoulos, Spyridon A., Soković, Marina, "Editorial: The chemistry of food in the advent of sustainable diets" in Frontiers in Nutrition, 9 (2022):1077985,
https://doi.org/10.3389/fnut.2022.1077985 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Finimundy, Tiane C.
AU  - Polyzos, Nikolaos
AU  - Pinela, José
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/639
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5119
AB  - In the present study, the chemical profile and bioactive properties of the roots of turnip-rooted parsley (Petroselinum crispum spp. tuberosum) germplasm were evaluated. For this purpose, plants from seventeen parsley cultivars were grown in 6 L pots, and the obtained roots were analyzed in terms of nutritional value, chemical composition (tocopherols, sugars and organic and fatty acids) and bioactive content (antioxidant activity, phenolic compound composition and antimicrobial properties). Our results showed great variability in terms of the chemical composition and bioactive properties of root parsley germplasm. A higher fresh root yield was recorded for the common “Root parsley” common variety (164 g/pot), followed by the varieties “Osborne” (109 g/pot), “Sonata” (104 g/pot), “Kaśka” (104 g/pot) and “Halblange Berlinska” (103 g/pot), whereas the lowest yield was recorded for the “Hanacka” variety (69 g/pot). A significant variation was also observed in the nutritional value parameters: the roots of the “Sonata” genotype showed the highest fat content; “Arat”, “Osborne” and “Olomuńcka” had the highest ash content; the “Alba” cultivar contained significantly higher amounts of carbohydrates; and the “Vistula” cultivar showed the highest energetic value. The only detected isoforms of vitamin E were α- and δ-tocopherols; content varied depending on the cultivar, although α-tocopherol was the most abundant compound in most cultivars, especially in the “Arat” cultivar. Sucrose was the most abundant free sugar detected, especially in the “Sonata” cultivar (16.96 g/100 g dw), followed by apiose (2.93–5.55 g/100 g dw), glucose (1.3–3.47 g/100 g dw) and fructose (1.37–3.03 g/100 g dw). Moreover, malic acid was the most abundant organic acid in most of the tested cultivars. Twenty-one individual fatty acids were identified in all the studied cultivars, with linoleic (47.9–57.1%) and palmitic acid (20.66–20.5%) being the most abundant. Nineteen individual phenolic compounds were tentatively identified, including three phenolic acids, fourteen flavonoids and two hydrolyzable tannins, while apigenin-O-pentoside-O-hexoside was the most abundant. The antioxidant activity differed between the tested assays (TBARS and OxHLIA), and the most effective cultivars for the TBARS assay (“Root parsley (common variety)” and “Berlinski Halblange Springer”) were those with the lowest antioxidant activity for the OxHLIA assay after 120 min. Finally, in most cases, the root extracts were more efficient or similarly effective compared to the positive controls against the tested bacteria and fungi. In conclusion, our results provide information regarding the chemical characterization and the bioactivities of the roots of turnip-rooted parsley germplasm that could be further exploited in sustainable and diversified agro-ecosystems through the introduction of this species as a novel/complementary crop in the traditional farming systems of the Mediterranean basin.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070639
SP  - 639
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Finimundy, Tiane C. and Polyzos, Nikolaos and Pinela, José and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2022",
abstract = "In the present study, the chemical profile and bioactive properties of the roots of turnip-rooted parsley (Petroselinum crispum spp. tuberosum) germplasm were evaluated. For this purpose, plants from seventeen parsley cultivars were grown in 6 L pots, and the obtained roots were analyzed in terms of nutritional value, chemical composition (tocopherols, sugars and organic and fatty acids) and bioactive content (antioxidant activity, phenolic compound composition and antimicrobial properties). Our results showed great variability in terms of the chemical composition and bioactive properties of root parsley germplasm. A higher fresh root yield was recorded for the common “Root parsley” common variety (164 g/pot), followed by the varieties “Osborne” (109 g/pot), “Sonata” (104 g/pot), “Kaśka” (104 g/pot) and “Halblange Berlinska” (103 g/pot), whereas the lowest yield was recorded for the “Hanacka” variety (69 g/pot). A significant variation was also observed in the nutritional value parameters: the roots of the “Sonata” genotype showed the highest fat content; “Arat”, “Osborne” and “Olomuńcka” had the highest ash content; the “Alba” cultivar contained significantly higher amounts of carbohydrates; and the “Vistula” cultivar showed the highest energetic value. The only detected isoforms of vitamin E were α- and δ-tocopherols; content varied depending on the cultivar, although α-tocopherol was the most abundant compound in most cultivars, especially in the “Arat” cultivar. Sucrose was the most abundant free sugar detected, especially in the “Sonata” cultivar (16.96 g/100 g dw), followed by apiose (2.93–5.55 g/100 g dw), glucose (1.3–3.47 g/100 g dw) and fructose (1.37–3.03 g/100 g dw). Moreover, malic acid was the most abundant organic acid in most of the tested cultivars. Twenty-one individual fatty acids were identified in all the studied cultivars, with linoleic (47.9–57.1%) and palmitic acid (20.66–20.5%) being the most abundant. Nineteen individual phenolic compounds were tentatively identified, including three phenolic acids, fourteen flavonoids and two hydrolyzable tannins, while apigenin-O-pentoside-O-hexoside was the most abundant. The antioxidant activity differed between the tested assays (TBARS and OxHLIA), and the most effective cultivars for the TBARS assay (“Root parsley (common variety)” and “Berlinski Halblange Springer”) were those with the lowest antioxidant activity for the OxHLIA assay after 120 min. Finally, in most cases, the root extracts were more efficient or similarly effective compared to the positive controls against the tested bacteria and fungi. In conclusion, our results provide information regarding the chemical characterization and the bioactivities of the roots of turnip-rooted parsley germplasm that could be further exploited in sustainable and diversified agro-ecosystems through the introduction of this species as a novel/complementary crop in the traditional farming systems of the Mediterranean basin.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070639",
pages = "639"
}

Petropoulos, S. A., Fernandes, Â., Finimundy, T. C., Polyzos, N., Pinela, J., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2022). The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm. in Horticulturae
Basel: MDPI., 8(7), 639.
https://doi.org/10.3390/horticulturae8070639

Petropoulos SA, Fernandes Â, Finimundy TC, Polyzos N, Pinela J, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm. in Horticulturae. 2022;8(7):639.
doi:10.3390/horticulturae8070639 .

Petropoulos, Spyridon A., Fernandes, Ângela, Finimundy, Tiane C., Polyzos, Nikolaos, Pinela, José, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Bioactivities and Chemical Profile of Turnip-Rooted Parsley Germplasm" in Horticulturae, 8, no. 7 (2022):639,
https://doi.org/10.3390/horticulturae8070639 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Santos-Buelga, Celestino
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2079-7737/11/5/699
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4980
AB  - Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.
PB  - Basel: MDPI
T2  - Biology
T1  - Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.
IS  - 5
VL  - 11
DO  - 10.3390/biology11050699
SP  - 699
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Santos-Buelga, Celestino and Barros, Lillian",
year = "2022",
abstract = "Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.",
number = "5",
volume = "11",
doi = "10.3390/biology11050699",
pages = "699"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Kostić, M., Soković, M., Ferreira, I. C. F. R., Santos-Buelga, C.,& Barros, L.. (2022). Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology
Basel: MDPI., 11(5), 699.
https://doi.org/10.3390/biology11050699

Mandim F, Petropoulos SA, Pinela J, Dias MI, Kostić M, Soković M, Ferreira ICFR, Santos-Buelga C, Barros L. Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology. 2022;11(5):699.
doi:10.3390/biology11050699 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Santos-Buelga, Celestino, Barros, Lillian, "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages." in Biology, 11, no. 5 (2022):699,
https://doi.org/10.3390/biology11050699 . .

TY  - CONF
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4929
AB  - Cynara cardunculus L. (cardoon) is a typical Mediterranean species that comprises the ancestor wild
cardoon (var. sylvestris), the cultivated leafy cardoon (var. altilis) and globe artichoke (var. scolymus). It
can be found worldwide due to its high adaptability to diverse climate conditions (e.g., resistance to extreme
temperatures, water stress, and soils with variable pH) [1,2]. It is also considered a multipurpose crop due
to its nutritional, pharmacological and industrial applications [2]. Due to the increase of its commercial and
economic value, this study aimed to evaluate the polyphenolic composition and bioactive potential of
cardoon bracts in relation to plant growth cycle. Cardoon bracts were collected in central Greece at eight
different maturation stages (samples C1 – lower maturity to C8 – higher maturity). The phenolic profile was
determined by HPLC-DAD-ESI/MS. The antioxidant activity was measured through two cell-based assays:
TBARS (thiobarbituric acid reactive substances formation inhibition) and OxHLIA (oxidative hemolysis
inhibition). Cytotoxic effects were screened against four human tumor cell lines and hepatotoxicity against
a non-tumor cell line (PLP2) by the sulforhodamine B assay. The anti-inflammatory potential was tested
through the inhibition of NO production by a murine macrophage cell line (RAW 264.7). Finally, the
antibacterial and antifungal activities were evaluated by the broth microdilution method. Twelve phenolic
compounds were tentatively identified in the cardoon bract extracts and quantified in higher amounts in
immature samples. Immature bracts (C1) also revealed the highest cytotoxic (GI50 of 30 – 79 μg/mL) and
anti-inflammatory (IC50 = 72 μg/mL) activities, while they presented the highest capacity to efficiently
inhibit the formation of TBARS (IC50 = 26.8 μg/mL). In contrast, the extract with the higher maturity grade
(C7) revealed superior efficacy against oxidative hemolysis (IC50 of 38 and 75 μg/mL at Δt=60 min and 120
min, respectively). The highest antibacterial and antifungal activities were attributed to samples C1 and C6
and samples C2 and C4, respectively. The results obtained in this work could be helpful to choose the best
harvesting time of cardoon bracts, allowing obtaining a greater variety of phenolic compounds, and
consequently, a higher bioactive potential, and ultimately the most appropriate use of their constituents.
Nevertheless, further studies are needed to better understand the compounds responsible for the observed
activities, as well as to reveal the mechanisms involved in these activities.
PB  - Bragança: Instituto Politécnico de Bragança
C3  - 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
T1  - Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4929
ER  - 

@conference{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2021",
abstract = "Cynara cardunculus L. (cardoon) is a typical Mediterranean species that comprises the ancestor wild
cardoon (var. sylvestris), the cultivated leafy cardoon (var. altilis) and globe artichoke (var. scolymus). It
can be found worldwide due to its high adaptability to diverse climate conditions (e.g., resistance to extreme
temperatures, water stress, and soils with variable pH) [1,2]. It is also considered a multipurpose crop due
to its nutritional, pharmacological and industrial applications [2]. Due to the increase of its commercial and
economic value, this study aimed to evaluate the polyphenolic composition and bioactive potential of
cardoon bracts in relation to plant growth cycle. Cardoon bracts were collected in central Greece at eight
different maturation stages (samples C1 – lower maturity to C8 – higher maturity). The phenolic profile was
determined by HPLC-DAD-ESI/MS. The antioxidant activity was measured through two cell-based assays:
TBARS (thiobarbituric acid reactive substances formation inhibition) and OxHLIA (oxidative hemolysis
inhibition). Cytotoxic effects were screened against four human tumor cell lines and hepatotoxicity against
a non-tumor cell line (PLP2) by the sulforhodamine B assay. The anti-inflammatory potential was tested
through the inhibition of NO production by a murine macrophage cell line (RAW 264.7). Finally, the
antibacterial and antifungal activities were evaluated by the broth microdilution method. Twelve phenolic
compounds were tentatively identified in the cardoon bract extracts and quantified in higher amounts in
immature samples. Immature bracts (C1) also revealed the highest cytotoxic (GI50 of 30 – 79 μg/mL) and
anti-inflammatory (IC50 = 72 μg/mL) activities, while they presented the highest capacity to efficiently
inhibit the formation of TBARS (IC50 = 26.8 μg/mL). In contrast, the extract with the higher maturity grade
(C7) revealed superior efficacy against oxidative hemolysis (IC50 of 38 and 75 μg/mL at Δt=60 min and 120
min, respectively). The highest antibacterial and antifungal activities were attributed to samples C1 and C6
and samples C2 and C4, respectively. The results obtained in this work could be helpful to choose the best
harvesting time of cardoon bracts, allowing obtaining a greater variety of phenolic compounds, and
consequently, a higher bioactive potential, and ultimately the most appropriate use of their constituents.
Nevertheless, further studies are needed to better understand the compounds responsible for the observed
activities, as well as to reveal the mechanisms involved in these activities.",
publisher = "Bragança: Instituto Politécnico de Bragança",
journal = "1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online",
title = "Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4929"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C.F.R.,& Barros, L.. (2021). Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
Bragança: Instituto Politécnico de Bragança., 47.
https://hdl.handle.net/21.15107/rcub_ibiss_4929

Mandim F, Petropoulos SA, Pinela J, Dias MI, Kostić M, Soković M, Santos-Buelga C, Ferreira IC, Barros L. Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online. 2021;:47.
https://hdl.handle.net/21.15107/rcub_ibiss_4929 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C.F.R., Barros, Lillian, "Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle" in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online (2021):47,
https://hdl.handle.net/21.15107/rcub_ibiss_4929 .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Giannoulis, Kyriakos D.
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Queijo, Beatriz
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814621018811
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4474
AB  - Cardoon seeds collected in Greece at four different maturity stages (samples S1 to S4) were analysed in terms of chemical composition and in vitro bioactivities. The content of phenolic compounds (six compounds in total) increased with increasing maturity, and 3,5-O-dicaffeyolquinic (14.8-33.8 mg/g extract) acid was the compound detected in higher abundance. Mature seeds (sample S4) also revealed the highest content in lipids (23 g/100 g extract) and tocopherols (29.62 mg/100 g dw) and demonstrated the highest cytotoxic (GI50 of 97-216 µg/mL) and anti-inflammatory (IC50 = 148 µg/mL) activities, and capacity to inhibit the formation of thiobarbituric acid reactive substances (TBARS) (IC50 = 5 µg/mL). Cardoon seed hydroethanolic extracts also revealed high antibacterial and antifungal potential, particularly samples S3 and S1, respectively. This study proved the multifaceted potential associated with valorisation of cardoon seeds, while their biological and chemical composition can be influenced by the maturity stage.
T2  - Food Chemistry
T1  - Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.
VL  - 369
DO  - 10.1016/j.foodchem.2021.130875
SP  - 130875
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Giannoulis, Kyriakos D. and Kostić, Marina and Soković, Marina and Queijo, Beatriz and Santos-Buelga, Celestino and Ferreira, Isabel C F R and Barros, Lillian",
year = "2021",
abstract = "Cardoon seeds collected in Greece at four different maturity stages (samples S1 to S4) were analysed in terms of chemical composition and in vitro bioactivities. The content of phenolic compounds (six compounds in total) increased with increasing maturity, and 3,5-O-dicaffeyolquinic (14.8-33.8 mg/g extract) acid was the compound detected in higher abundance. Mature seeds (sample S4) also revealed the highest content in lipids (23 g/100 g extract) and tocopherols (29.62 mg/100 g dw) and demonstrated the highest cytotoxic (GI50 of 97-216 µg/mL) and anti-inflammatory (IC50 = 148 µg/mL) activities, and capacity to inhibit the formation of thiobarbituric acid reactive substances (TBARS) (IC50 = 5 µg/mL). Cardoon seed hydroethanolic extracts also revealed high antibacterial and antifungal potential, particularly samples S3 and S1, respectively. This study proved the multifaceted potential associated with valorisation of cardoon seeds, while their biological and chemical composition can be influenced by the maturity stage.",
journal = "Food Chemistry",
title = "Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.",
volume = "369",
doi = "10.1016/j.foodchem.2021.130875",
pages = "130875"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Giannoulis, K. D., Kostić, M., Soković, M., Queijo, B., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.. in Food Chemistry, 369, 130875.
https://doi.org/10.1016/j.foodchem.2021.130875

Mandim F, Petropoulos SA, Pinela J, Dias MI, Giannoulis KD, Kostić M, Soković M, Queijo B, Santos-Buelga C, Ferreira ICFR, Barros L. Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.. in Food Chemistry. 2021;369:130875.
doi:10.1016/j.foodchem.2021.130875 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Giannoulis, Kyriakos D., Kostić, Marina, Soković, Marina, Queijo, Beatriz, Santos-Buelga, Celestino, Ferreira, Isabel C F R, Barros, Lillian, "Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages." in Food Chemistry, 369 (2021):130875,
https://doi.org/10.1016/j.foodchem.2021.130875 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2073-4395/11/3/576
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4419
AB  - The Mediterranean basin is abundant in wild edible species with numerous health beneficial effects due to the presence of various bioactive phytochemicals. In the present work, the effect of nitrogen fertilization rates (0 ppm, (N0), 200 ppm (N1), 400 ppm (N2), and 600 ppm (N3) of total N) and growth substrate composition (soil or peat/perlite (2/1; v/v)) on the chemical composition and bioactive properties of Centaurea raphanina ssp. mixta plants was evaluated. The results of the study showed that both the tested factors affected nutritional value of the edible leaves, with the soil × N1 treatment being the most beneficial for fat, protein, and carbohydrate content and energetic value. On the other hand, the peat/perlite-grown plants that received 200 ppm of N had the highest content in α-, γ-, and total tocopherols, while the control treatment of soil-grown plants was the richest in individual and total sugars. Oxalic, citric, and total organic acids were the highest in the N2 × soil treatment, while malic acid was the highest in control treatment of the same substrate. The main fatty acids were palmitic, α-linolenic, and linoleic acids, with the highest contents being observed in the N0 × soil, N3 × soil, and N3 × peat/perlite treatments, respectively. The major phenolic compounds were pinocembrim neohesperidoside and pinocembrim acetyl neohesperidoside isomer II, with the highest content being observed in the N1 × soil treatment. The highest antihemolytic activity was observed in the N3 × peat/perlite treatment, while the most effective treatments against lipid peroxidation were N0 (in both soil and peat/perlite combinations) and N1 × peat/perlite. Lastly, all the tested extracts (except for N1 × soil) showed promising cytotoxic effects against HeLa (cervical carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), and NCI-H460 (non-small-cell lung cancer), while all the tested extracts exhibited better antifungal activities (lower minimal inhibition concentration (MIC) values) against Trichoderma viride than the positive controls. Overall, the present results suggest that the application of cost-effective practices such as the nitrogen application and the selection of growth substrate may regulate the chemical composition and the bioactive properties of C. raphanina ssp. mixta species and increase its added value under commercial cultivation conditions.
T2  - Agronomy
T1  - Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark
IS  - 3
VL  - 11
DO  - 10.3390/agronomy11030576
SP  - 576
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "The Mediterranean basin is abundant in wild edible species with numerous health beneficial effects due to the presence of various bioactive phytochemicals. In the present work, the effect of nitrogen fertilization rates (0 ppm, (N0), 200 ppm (N1), 400 ppm (N2), and 600 ppm (N3) of total N) and growth substrate composition (soil or peat/perlite (2/1; v/v)) on the chemical composition and bioactive properties of Centaurea raphanina ssp. mixta plants was evaluated. The results of the study showed that both the tested factors affected nutritional value of the edible leaves, with the soil × N1 treatment being the most beneficial for fat, protein, and carbohydrate content and energetic value. On the other hand, the peat/perlite-grown plants that received 200 ppm of N had the highest content in α-, γ-, and total tocopherols, while the control treatment of soil-grown plants was the richest in individual and total sugars. Oxalic, citric, and total organic acids were the highest in the N2 × soil treatment, while malic acid was the highest in control treatment of the same substrate. The main fatty acids were palmitic, α-linolenic, and linoleic acids, with the highest contents being observed in the N0 × soil, N3 × soil, and N3 × peat/perlite treatments, respectively. The major phenolic compounds were pinocembrim neohesperidoside and pinocembrim acetyl neohesperidoside isomer II, with the highest content being observed in the N1 × soil treatment. The highest antihemolytic activity was observed in the N3 × peat/perlite treatment, while the most effective treatments against lipid peroxidation were N0 (in both soil and peat/perlite combinations) and N1 × peat/perlite. Lastly, all the tested extracts (except for N1 × soil) showed promising cytotoxic effects against HeLa (cervical carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), and NCI-H460 (non-small-cell lung cancer), while all the tested extracts exhibited better antifungal activities (lower minimal inhibition concentration (MIC) values) against Trichoderma viride than the positive controls. Overall, the present results suggest that the application of cost-effective practices such as the nitrogen application and the selection of growth substrate may regulate the chemical composition and the bioactive properties of C. raphanina ssp. mixta species and increase its added value under commercial cultivation conditions.",
journal = "Agronomy",
title = "Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark",
number = "3",
volume = "11",
doi = "10.3390/agronomy11030576",
pages = "576"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark. in Agronomy, 11(3), 576.
https://doi.org/10.3390/agronomy11030576

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark. in Agronomy. 2021;11(3):576.
doi:10.3390/agronomy11030576 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "Effects of Growing Substrate and Nitrogen Fertilization on the Chemical Composition and Bioactive Properties of Centaurea raphanina ssp. mixta (DC.) Runemark" in Agronomy, 11, no. 3 (2021):576,
https://doi.org/10.3390/agronomy11030576 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32781352
UR  - https://radar.ibiss.bg.ac.rs/123456789/3846
AB  - Cardoon (Cynara cardunculus L.) bracts were collected at different maturation stages to investigate seasonal changes in the phenolic compounds profile and in vitro bioactivities. Among the 12 phenolic compounds tentatively identified, 3,5-O-dicaffeoylquinic acid (21.83 mg/g extract) and apigenin-7-O-glucuronide (10.6 mg/g extract) were the most abundant. Immature bracts (C1: principal growth stage (PGS) 5) had the highest phenolic compounds content, and anti-inflammatory (IC50 = 72 µg/mL) and cytotoxic (GI50 of 30-79 µg/mL) activities. Moreover, extract C1 inhibited efficiently the formation of thiobarbituric acid reactive substances (TBARS; IC50 = 26.8 µg/mL), while extract C8 (PGS 8/9) was more effective against oxidative haemolysis (IC50 38 and 75 µg/mL). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 (PGS 7/8) and samples C2 (PGS 5/6) and C4 (PGS 6/7), respectively. Overall, the obtained results suggest the seasonal changes of polyphenolic composition and bioactivity of cardoon bracts of variable maturity.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.
VL  - 336
DO  - 10.1016/j.foodchem.2020.127744
SP  - 127744
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C F R and Barros, Lillian",
year = "2021",
abstract = "Cardoon (Cynara cardunculus L.) bracts were collected at different maturation stages to investigate seasonal changes in the phenolic compounds profile and in vitro bioactivities. Among the 12 phenolic compounds tentatively identified, 3,5-O-dicaffeoylquinic acid (21.83 mg/g extract) and apigenin-7-O-glucuronide (10.6 mg/g extract) were the most abundant. Immature bracts (C1: principal growth stage (PGS) 5) had the highest phenolic compounds content, and anti-inflammatory (IC50 = 72 µg/mL) and cytotoxic (GI50 of 30-79 µg/mL) activities. Moreover, extract C1 inhibited efficiently the formation of thiobarbituric acid reactive substances (TBARS; IC50 = 26.8 µg/mL), while extract C8 (PGS 8/9) was more effective against oxidative haemolysis (IC50 38 and 75 µg/mL). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 (PGS 7/8) and samples C2 (PGS 5/6) and C4 (PGS 6/7), respectively. Overall, the obtained results suggest the seasonal changes of polyphenolic composition and bioactivity of cardoon bracts of variable maturity.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.",
volume = "336",
doi = "10.1016/j.foodchem.2020.127744",
pages = "127744"
}

Mandim, F., Petropoulos, S. A., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.. in Food Chemistry
Elsevier Ltd., 336, 127744.
https://doi.org/10.1016/j.foodchem.2020.127744

Mandim F, Petropoulos SA, Dias MI, Pinela J, Kostić M, Soković M, Santos-Buelga C, Ferreira ICFR, Barros L. Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.. in Food Chemistry. 2021;336:127744.
doi:10.1016/j.foodchem.2020.127744 .

Mandim, Filipa, Petropoulos, Spyridon A., Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C F R, Barros, Lillian, "Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts." in Food Chemistry, 336 (2021):127744,
https://doi.org/10.1016/j.foodchem.2020.127744 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Figueiredo, Sara
AU  - Finimundy, Tiane C.
AU  - Pinela, José
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Petropoulos, Spyridon A.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2071-1050/13/12/6869
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4418
AB  - Biostimulants are a novel and eco-friendly agronomic tool with practical applications in alleviating negative effects of environmental stressors. The present work studied the effects of three biostimulant products (Nomoren (N), Twin-Antistress (TW), and X-Stress (XS)) under normal irrigation (W+) and water deficit irrigation conditions (W−) on the nutritional, chemical composition and bioactive properties of common bean fresh pods. A variable effect of biostimulants and water deficit irrigation was observed on nutritional value parameters, while fructose and sucrose were the main detected sugars, especially in NW+ and CW− treatments. Oxalic, malic, and citric acid were the main detected organic acids, while γ- and total tocopherol content was the highest in TWW+. (+)-Catechin and (−)-epicatechin were the most abundant phenolic compounds, especially in the NW− treatment. A variable antioxidant capacity was observed for the Thiobarbituric Acid Reactive Substances (TBARS) and Oxidative Haemolysis assays (OxHLIA), while TWW+ extracts showed the best overall results against the tested fungi. In conclusion, the tested biostimulants had a positive effect on chemical composition and bioactivities of purple bean depending on the irrigation regime.
T2  - Sustainability
T1  - Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application
IS  - 12
VL  - 13
DO  - 10.3390/su13126869
SP  - 6869
ER  - 

@article{
author = "Fernandes, Ângela and Figueiredo, Sara and Finimundy, Tiane C. and Pinela, José and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Petropoulos, Spyridon A. and Barros, Lillian",
year = "2021",
abstract = "Biostimulants are a novel and eco-friendly agronomic tool with practical applications in alleviating negative effects of environmental stressors. The present work studied the effects of three biostimulant products (Nomoren (N), Twin-Antistress (TW), and X-Stress (XS)) under normal irrigation (W+) and water deficit irrigation conditions (W−) on the nutritional, chemical composition and bioactive properties of common bean fresh pods. A variable effect of biostimulants and water deficit irrigation was observed on nutritional value parameters, while fructose and sucrose were the main detected sugars, especially in NW+ and CW− treatments. Oxalic, malic, and citric acid were the main detected organic acids, while γ- and total tocopherol content was the highest in TWW+. (+)-Catechin and (−)-epicatechin were the most abundant phenolic compounds, especially in the NW− treatment. A variable antioxidant capacity was observed for the Thiobarbituric Acid Reactive Substances (TBARS) and Oxidative Haemolysis assays (OxHLIA), while TWW+ extracts showed the best overall results against the tested fungi. In conclusion, the tested biostimulants had a positive effect on chemical composition and bioactivities of purple bean depending on the irrigation regime.",
journal = "Sustainability",
title = "Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application",
number = "12",
volume = "13",
doi = "10.3390/su13126869",
pages = "6869"
}

Fernandes, Â., Figueiredo, S., Finimundy, T. C., Pinela, J., Tzortzakis, N., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Petropoulos, S. A.,& Barros, L.. (2021). Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application. in Sustainability, 13(12), 6869.
https://doi.org/10.3390/su13126869

Fernandes Â, Figueiredo S, Finimundy TC, Pinela J, Tzortzakis N, Ivanov M, Soković M, Ferreira ICFR, Petropoulos SA, Barros L. Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application. in Sustainability. 2021;13(12):6869.
doi:10.3390/su13126869 .

Fernandes, Ângela, Figueiredo, Sara, Finimundy, Tiane C., Pinela, José, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Petropoulos, Spyridon A., Barros, Lillian, "Chemical Composition and Bioactive Properties of Purple French Bean (Phaseolus vulgaris L.) as Affected by Water Deficit Irrigation and Biostimulants Application" in Sustainability, 13, no. 12 (2021):6869,
https://doi.org/10.3390/su13126869 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/12/1907
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8750300
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4741
AB  - Hydroethanolic extracts of cardoon petioles collected at sixteen growth stages (P1-P16) were characterized in terms of their phenolic composition and bioactive potential (antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities). Fifteen phenolic compounds were tentatively identified (i.e., ten phenolic acids and five flavonoid glycosides); the main compounds were 5-O-caffeoylquinic and 1,5-di-O-caffeoylquinic acids. Samples collected at early maturity (P1-P4) presented a weak positive correlation between the higher content in polyphenols (P3: 101-mg/g extract) and better inhibition capacity against thiobarbituric acid reactive substance formation (TBARS; P3: IC50 = 5.0 µg/mL). Samples at intermediate maturation stages (P9) presented higher cytotoxic and anti-inflammatory potential. Moreover, immature petioles showed greater antihemolytic (OxHLIA; P4: IC50 = 65 and 180 µg/mL for Δt of 60 and 120 min, respectively) and antibacterial activity. The antifungal activity varied depending on the maturation stage and the fungi strain. In conclusion, the maturation stage may greatly affect the polyphenols composition and content and the bioactive potential of cardoon petioles.
PB  - Basel: MDPI
T2  - Antioxidants (Basel, Switzerland)
T1  - Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.
IS  - 12
VL  - 10
DO  - 10.3390/antiox10121907
SP  - 1907
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Hydroethanolic extracts of cardoon petioles collected at sixteen growth stages (P1-P16) were characterized in terms of their phenolic composition and bioactive potential (antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities). Fifteen phenolic compounds were tentatively identified (i.e., ten phenolic acids and five flavonoid glycosides); the main compounds were 5-O-caffeoylquinic and 1,5-di-O-caffeoylquinic acids. Samples collected at early maturity (P1-P4) presented a weak positive correlation between the higher content in polyphenols (P3: 101-mg/g extract) and better inhibition capacity against thiobarbituric acid reactive substance formation (TBARS; P3: IC50 = 5.0 µg/mL). Samples at intermediate maturation stages (P9) presented higher cytotoxic and anti-inflammatory potential. Moreover, immature petioles showed greater antihemolytic (OxHLIA; P4: IC50 = 65 and 180 µg/mL for Δt of 60 and 120 min, respectively) and antibacterial activity. The antifungal activity varied depending on the maturation stage and the fungi strain. In conclusion, the maturation stage may greatly affect the polyphenols composition and content and the bioactive potential of cardoon petioles.",
publisher = "Basel: MDPI",
journal = "Antioxidants (Basel, Switzerland)",
title = "Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.",
number = "12",
volume = "10",
doi = "10.3390/antiox10121907",
pages = "1907"
}

Mandim, F., Petropoulos, S. A., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.. in Antioxidants (Basel, Switzerland)
Basel: MDPI., 10(12), 1907.
https://doi.org/10.3390/antiox10121907

Mandim F, Petropoulos SA, Dias MI, Pinela J, Kostić M, Soković M, Santos-Buelga C, Ferreira ICFR, Barros L. Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.. in Antioxidants (Basel, Switzerland). 2021;10(12):1907.
doi:10.3390/antiox10121907 .

Mandim, Filipa, Petropoulos, Spyridon A., Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C. F. R., Barros, Lillian, "Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage." in Antioxidants (Basel, Switzerland), 10, no. 12 (2021):1907,
https://doi.org/10.3390/antiox10121907 . .

TY  - JOUR
AU  - Sampaio, Shirley L.
AU  - Lonchamp, Julien
AU  - Dias, Maria Inês
AU  - Liddle, Catriona
AU  - Petropoulos, Spyridon A.
AU  - Glamočlija, Jasmina
AU  - Alexopoulos, Alexios
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814620323888
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4041
AB  - Aqueous extracts from seven coloured potato varieties (three red-fleshed, three-purple fleshed, and one marble-fleshed) were studied for their anthocyanin content, in vitro biological activities, colouring properties and their potential application in the food industry. Acylated glycosides or pelargonidin and petunidin aglycones were identified as the main anthocyanin forms in the red and purple varieties, respectively. The total anthocyanin content among varieties ranged from 478.3 to 886.2 mg/100 g extract. All the extracts presented in vitro antioxidant, antibacterial and antifungal activities, whereas no toxic effects were detected. Finally, two selected extracts were tested as colourants in a soft drink formulation and presented suitable sensory profiles as well as high colour stability during a 30-day shelf-life when compared with the commercial colourant E163. Therefore, the tested extracts could be used as natural food colourants and considered for substituting the existing synthetic colouring agents.
PB  - Elsevier BV
T2  - Food Chemistry
T1  - Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.
VL  - 342
DO  - 10.1016/j.foodchem.2020.128526
SP  - 128526
ER  - 

@article{
author = "Sampaio, Shirley L. and Lonchamp, Julien and Dias, Maria Inês and Liddle, Catriona and Petropoulos, Spyridon A. and Glamočlija, Jasmina and Alexopoulos, Alexios and Santos-Buelga, Celestino and Ferreira, Isabel C F R and Barros, Lillian",
year = "2021",
abstract = "Aqueous extracts from seven coloured potato varieties (three red-fleshed, three-purple fleshed, and one marble-fleshed) were studied for their anthocyanin content, in vitro biological activities, colouring properties and their potential application in the food industry. Acylated glycosides or pelargonidin and petunidin aglycones were identified as the main anthocyanin forms in the red and purple varieties, respectively. The total anthocyanin content among varieties ranged from 478.3 to 886.2 mg/100 g extract. All the extracts presented in vitro antioxidant, antibacterial and antifungal activities, whereas no toxic effects were detected. Finally, two selected extracts were tested as colourants in a soft drink formulation and presented suitable sensory profiles as well as high colour stability during a 30-day shelf-life when compared with the commercial colourant E163. Therefore, the tested extracts could be used as natural food colourants and considered for substituting the existing synthetic colouring agents.",
publisher = "Elsevier BV",
journal = "Food Chemistry",
title = "Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.",
volume = "342",
doi = "10.1016/j.foodchem.2020.128526",
pages = "128526"
}

Sampaio, S. L., Lonchamp, J., Dias, M. I., Liddle, C., Petropoulos, S. A., Glamočlija, J., Alexopoulos, A., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.. in Food Chemistry
Elsevier BV., 342, 128526.
https://doi.org/10.1016/j.foodchem.2020.128526

Sampaio SL, Lonchamp J, Dias MI, Liddle C, Petropoulos SA, Glamočlija J, Alexopoulos A, Santos-Buelga C, Ferreira ICFR, Barros L. Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis.. in Food Chemistry. 2021;342:128526.
doi:10.1016/j.foodchem.2020.128526 .

Sampaio, Shirley L., Lonchamp, Julien, Dias, Maria Inês, Liddle, Catriona, Petropoulos, Spyridon A., Glamočlija, Jasmina, Alexopoulos, Alexios, Santos-Buelga, Celestino, Ferreira, Isabel C F R, Barros, Lillian, "Anthocyanin-rich extracts from purple and red potatoes as natural colourants: Bioactive properties, application in a soft drink formulation and sensory analysis." in Food Chemistry, 342 (2021):128526,
https://doi.org/10.1016/j.foodchem.2020.128526 . .

TY  - JOUR
AU  - Cruz, Luís R. O.
AU  - Fernandes, Ângela
AU  - Di Gioia, Francesco
AU  - Petropoulos, Spyridon A.
AU  - Polyzos, Nikolaos
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/11/1036
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3967
AB  - In the present study, three red-colored (Dark Opal, Basilico Rosso, and Red Basil) and one green-colored landrace (Mitikas) of basil (Ocimum basilicum L.) were grown under four nitrogen regimes, namely Control (no fertilizer added), 200 ppm, 400 ppm, and 600 ppm of nitrogen (N). Fresh yield varied depending on N input following a quadratic function in all four genotypes, and green basil performed better compared to the red cultivars. A significant interaction of genotype × N input was recorded for most of the chemical parameters measured. Tocopherols contents of leaves were consistently higher in plants that received 200 ppm of N and lower in those receiving 600 ppm of N, especially in Dark Opal and Red Basil cultivars. Polyunsaturated fatty acids (PUFA) were the major category of fatty acids and Red Basil had the lowest ratio of omega-6/omega 3 (0.29) and thus the best fatty acid profile. Polyphenols content was the highest in Red Basil and Dark Opal (25 mg/g of extract on average) and the lowest in Mitikas and decreased with increasing N input. Similarly, antioxidant activity was the highest in Dark Opal and Red Basil fertigated with 200 ppm of N, whereas all the leaf extracts tested had good antibacterial and antifungal activity. In conclusion, basil chemical and bioactive profile was significantly influenced by both genotype and N input. Red-colored basil, although less productive, had the best chemical profile, and moderate levels of N input may provide the best compromise between yield, nutritional value, and bioactivity for the species.
PB  - MDPI AG
T2  - Antioxidants
T1  - The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars
IS  - 11
VL  - 9
DO  - 10.3390/antiox9111036
SP  - 1036
ER  - 

@article{
author = "Cruz, Luís R. O. and Fernandes, Ângela and Di Gioia, Francesco and Petropoulos, Spyridon A. and Polyzos, Nikolaos and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "In the present study, three red-colored (Dark Opal, Basilico Rosso, and Red Basil) and one green-colored landrace (Mitikas) of basil (Ocimum basilicum L.) were grown under four nitrogen regimes, namely Control (no fertilizer added), 200 ppm, 400 ppm, and 600 ppm of nitrogen (N). Fresh yield varied depending on N input following a quadratic function in all four genotypes, and green basil performed better compared to the red cultivars. A significant interaction of genotype × N input was recorded for most of the chemical parameters measured. Tocopherols contents of leaves were consistently higher in plants that received 200 ppm of N and lower in those receiving 600 ppm of N, especially in Dark Opal and Red Basil cultivars. Polyunsaturated fatty acids (PUFA) were the major category of fatty acids and Red Basil had the lowest ratio of omega-6/omega 3 (0.29) and thus the best fatty acid profile. Polyphenols content was the highest in Red Basil and Dark Opal (25 mg/g of extract on average) and the lowest in Mitikas and decreased with increasing N input. Similarly, antioxidant activity was the highest in Dark Opal and Red Basil fertigated with 200 ppm of N, whereas all the leaf extracts tested had good antibacterial and antifungal activity. In conclusion, basil chemical and bioactive profile was significantly influenced by both genotype and N input. Red-colored basil, although less productive, had the best chemical profile, and moderate levels of N input may provide the best compromise between yield, nutritional value, and bioactivity for the species.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars",
number = "11",
volume = "9",
doi = "10.3390/antiox9111036",
pages = "1036"
}

Cruz, L. R. O., Fernandes, Â., Di Gioia, F., Petropoulos, S. A., Polyzos, N., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars. in Antioxidants
MDPI AG., 9(11), 1036.
https://doi.org/10.3390/antiox9111036

Cruz LRO, Fernandes Â, Di Gioia F, Petropoulos SA, Polyzos N, Dias MI, Pinela J, Kostić M, Soković M, Ferreira ICFR, Barros L. The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars. in Antioxidants. 2020;9(11):1036.
doi:10.3390/antiox9111036 .

Cruz, Luís R. O., Fernandes, Ângela, Di Gioia, Francesco, Petropoulos, Spyridon A., Polyzos, Nikolaos, Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Effect of Nitrogen Input on Chemical Profile and Bioactive Properties of Green- and Red-Colored Basil Cultivars" in Antioxidants, 9, no. 11 (2020):1036,
https://doi.org/10.3390/antiox9111036 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Plexida, Sofia
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2071-1050/12/20/8511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3943
AB  - The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.
PB  - MDPI AG
T2  - Sustainability
T1  - The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production
IS  - 20
VL  - 12
DO  - 10.3390/su12208511
SP  - 8511
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Plexida, Sofia and Pereira, Carla and Dias, Maria Inês and Calhelha, Ricardo and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Petrović, Jovana and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "The environmental burden from crop production byproducts is gradually increasing and necessitates the sustainable management of waste towards a circular economy approach. In the present study, three byproducts (cotton ginning waste (CGW), ground hazelnut husks (GHH) and ground peanut husks (GPH)) were evaluated in lettuce cultivation. For this purpose, the tested materials were incorporated in soil at two different rates (25% and 50% of total substrate volume) while a control treatment (no addition of byproducts) was also considered. Fresh weight per plant and total yield was the highest for the GHH50% treatment. The highest fat, protein, carbohydrates and energy content were observed for the CGW25% treatment. Chemical composition also differed among the tested byproducts where CGW25% treatment had the highest total tocopherols, sugars (sucrose, fructose, trehalose and total sugars) and organic acids content. The most abundant fatty acids were α-linolenic, linoleic and palmitic acid in all the tested treatments, while the highest antioxidant activity was observed for the GHH50% treatment. Regarding polyphenols, phenolic acids content was the highest in the GHH treatments, whereas flavonoids were the highest for the CGW25% treatment. No cytotoxicity against the PLP2 non-tumor cell line was observed, whereas only the GPH50% treatment showed moderate efficacy against HeLa, HepG2 and MCF-7 cell lines. The tested extracts also showed moderate antibacterial activities and only the extracts from the CGW50% treatment were more effective than the positive control against Trichoderma viride. In conclusion, the present results showed the great potential of using the tested byproducts as soil amendments for vegetable crops production, since they may improve the nutritional parameters, the chemical profile and the bioactivities of the final product. The suggested alternative use of the tested byproducts not only will increase the added value of crops but will also alleviate the environmental burden from bulky agroindustry byproducts.",
publisher = "MDPI AG",
journal = "Sustainability",
title = "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production",
number = "20",
volume = "12",
doi = "10.3390/su12208511",
pages = "8511"
}

Petropoulos, S. A., Fernandes, Â., Plexida, S., Pereira, C., Dias, M. I., Calhelha, R., Chrysargyris, A., Tzortzakis, N., Petrović, J., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability
MDPI AG., 12(20), 8511.
https://doi.org/10.3390/su12208511

Petropoulos SA, Fernandes Â, Plexida S, Pereira C, Dias MI, Calhelha R, Chrysargyris A, Tzortzakis N, Petrović J, Soković M, Ferreira ICFR, Barros L. The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production. in Sustainability. 2020;12(20):8511.
doi:10.3390/su12208511 .

Petropoulos, Spyridon A., Fernandes, Ângela, Plexida, Sofia, Pereira, Carla, Dias, Maria Inês, Calhelha, Ricardo, Chrysargyris, Antonios, Tzortzakis, Nikolaos, Petrović, Jovana, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "The Sustainable Use of Cotton, Hazelnut and Ground Peanut Waste in Vegetable Crop Production" in Sustainability, 12, no. 20 (2020):8511,
https://doi.org/10.3390/su12208511 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3175
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32664565
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3829
AB  - The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143175
SP  - 3175
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C F R and Barros, Lillian",
year = "2020",
abstract = "The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143175",
pages = "3175"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Ivanov, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3175.
https://doi.org/10.3390/molecules25143175

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Ivanov M, Soković M, Ferreira ICFR, Barros L. The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark.. in Molecules (Basel, Switzerland). 2020;25(14):3175.
doi:10.3390/molecules25143175 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Ferreira, Isabel C F R, Barros, Lillian, "The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3175,
https://doi.org/10.3390/molecules25143175 . .

TY  - JOUR
AU  - Finimundy, Tiane C.
AU  - Karkanis, Anestis
AU  - Fernandes, Ângela
AU  - Petropoulos, Spyridon A.
AU  - Calhelha, Ricardo
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Rosa, Eduardo
AU  - Barros, Lillian
AU  - Ferreira, Isabel C F R
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32470801
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3694
AB  - In this study, the chemical characterization and bioactive properties of S. minor cultivated under different fertilization rates (control, half rate and full rate) were evaluated. Twenty-two phenolic compounds were identified, including five phenolic acids, seven flavonoids and ten tannins. Hydrolysable tannins were prevalent, namely Sanguiin H-10, especially in leaves without fertilization (control). Roots of full-rate fertilizer (660 Kg/ha) presented the highest flavonoid content, mainly catechin and its isomers, whereas half-rate fertilizer (330 Kg/ha), presented the highest content of total phenolic compounds, due to the higher amount of ellagitannins (lambertianin C: 84 ± 1 mg/g of dry extract). Antimicrobial activities were also promising, especially against Salmonella typhimurium (MBC = 0.44 mg/mL). Moreover, root samples revealed activity against all tested cell lines regardless of fertilization rate, whereas leaves were effective only against HeLa cell line. In conclusion, S. minor could be a source of natural bioactive compounds, while fertilization could increase phenolic compounds content.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.
VL  - 327
DO  - 10.1016/j.foodchem.2020.127043
SP  - 127043
ER  - 

@article{
author = "Finimundy, Tiane C. and Karkanis, Anestis and Fernandes, Ângela and Petropoulos, Spyridon A. and Calhelha, Ricardo and Petrović, Jovana and Soković, Marina and Rosa, Eduardo and Barros, Lillian and Ferreira, Isabel C F R",
year = "2020",
abstract = "In this study, the chemical characterization and bioactive properties of S. minor cultivated under different fertilization rates (control, half rate and full rate) were evaluated. Twenty-two phenolic compounds were identified, including five phenolic acids, seven flavonoids and ten tannins. Hydrolysable tannins were prevalent, namely Sanguiin H-10, especially in leaves without fertilization (control). Roots of full-rate fertilizer (660 Kg/ha) presented the highest flavonoid content, mainly catechin and its isomers, whereas half-rate fertilizer (330 Kg/ha), presented the highest content of total phenolic compounds, due to the higher amount of ellagitannins (lambertianin C: 84 ± 1 mg/g of dry extract). Antimicrobial activities were also promising, especially against Salmonella typhimurium (MBC = 0.44 mg/mL). Moreover, root samples revealed activity against all tested cell lines regardless of fertilization rate, whereas leaves were effective only against HeLa cell line. In conclusion, S. minor could be a source of natural bioactive compounds, while fertilization could increase phenolic compounds content.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.",
volume = "327",
doi = "10.1016/j.foodchem.2020.127043",
pages = "127043"
}

Finimundy, T. C., Karkanis, A., Fernandes, Â., Petropoulos, S. A., Calhelha, R., Petrović, J., Soković, M., Rosa, E., Barros, L.,& Ferreira, I. C. F. R.. (2020). Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.. in Food Chemistry
Elsevier Ltd., 327, 127043.
https://doi.org/10.1016/j.foodchem.2020.127043

Finimundy TC, Karkanis A, Fernandes Â, Petropoulos SA, Calhelha R, Petrović J, Soković M, Rosa E, Barros L, Ferreira ICFR. Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes.. in Food Chemistry. 2020;327:127043.
doi:10.1016/j.foodchem.2020.127043 .

Finimundy, Tiane C., Karkanis, Anestis, Fernandes, Ângela, Petropoulos, Spyridon A., Calhelha, Ricardo, Petrović, Jovana, Soković, Marina, Rosa, Eduardo, Barros, Lillian, Ferreira, Isabel C F R, "Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimes." in Food Chemistry, 327 (2020):127043,
https://doi.org/10.1016/j.foodchem.2020.127043 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Ines
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/9/2204
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3675
AB  - The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.
PB  - MDPI AG
T2  - Molecules
T1  - Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions
IS  - 9
VL  - 25
DO  - 10.3390/molecules25092204
SP  - 2204
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Ines and Pereira, Carla and Calhelha, Ricardo C. and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.",
publisher = "MDPI AG",
journal = "Molecules",
title = "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions",
number = "9",
volume = "25",
doi = "10.3390/molecules25092204",
pages = "2204"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R. C., Chrysargyris, A., Tzortzakis, N., Ivanov, M., Soković, M., Barros, L.,& Ferreira, I. C. F. R.. (2020). Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules
MDPI AG., 25(9), 2204.
https://doi.org/10.3390/molecules25092204

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha RC, Chrysargyris A, Tzortzakis N, Ivanov M, Soković M, Barros L, Ferreira ICFR. Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions. in Molecules. 2020;25(9):2204.
doi:10.3390/molecules25092204 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Ines, Pereira, Carla, Calhelha, Ricardo C., Chrysargyris, Antonios, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C. F. R., "Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions" in Molecules, 25, no. 9 (2020):2204,
https://doi.org/10.3390/molecules25092204 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Giannoulis, Kyriakos D.
AU  - Dias, Maria Inês
AU  - Fernandes, Ângela
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996920303069
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3674
AB  - Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.
PB  - Elsevier Ltd
T2  - Food Research International
T1  - Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis
VL  - 134
DO  - 10.1016/j.foodres.2020.109281
SP  - 109281
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Giannoulis, Kyriakos D. and Dias, Maria Inês and Fernandes, Ângela and Pinela, José and Kostić, Marina and Soković, Marina and Barros, Lillian and Santos-Buelga, Celestino and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.",
publisher = "Elsevier Ltd",
journal = "Food Research International",
title = "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis",
volume = "134",
doi = "10.1016/j.foodres.2020.109281",
pages = "109281"
}

Mandim, F., Petropoulos, S. A., Giannoulis, K. D., Dias, M. I., Fernandes, Â., Pinela, J., Kostić, M., Soković, M., Barros, L., Santos-Buelga, C.,& Ferreira, I. C.F.R.. (2020). Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International
Elsevier Ltd., 134, 109281.
https://doi.org/10.1016/j.foodres.2020.109281

Mandim F, Petropoulos SA, Giannoulis KD, Dias MI, Fernandes Â, Pinela J, Kostić M, Soković M, Barros L, Santos-Buelga C, Ferreira IC. Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International. 2020;134:109281.
doi:10.1016/j.foodres.2020.109281 .

Mandim, Filipa, Petropoulos, Spyridon A., Giannoulis, Kyriakos D., Dias, Maria Inês, Fernandes, Ângela, Pinela, José, Kostić, Marina, Soković, Marina, Barros, Lillian, Santos-Buelga, Celestino, Ferreira, Isabel C.F.R., "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis" in Food Research International, 134 (2020):109281,
https://doi.org/10.1016/j.foodres.2020.109281 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Dias, Maria Ines
AU  - Pereira, Carla
AU  - Calhelha, Ricardo
AU  - Di Gioia, Francesco
AU  - Tzortzakis, Nikolaos
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/4/314
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3664
AB  - Centaurea raphanina subsp. mixta (DC.) Runemark is a wild edible species endemic to Greece. This study evaluated the chemical composition and bioactive properties of wild and cultivated C. raphanina subsp. mixta plants. Wild plants had higher nutritional value than cultivated ones, whereas cultivated plants contained more tocopherols. Glucose and sucrose were higher in cultivated plants and trehalose in wild ones. Oxalic and total organic acids were detected in higher amounts in cultivated samples. The main fatty acids were α-linolenic, linoleic and palmitic acid, while wild plants were richer in polyunsaturated fatty acids. Two pinocembrin derivatives were the main phenolic compounds being detected in higher amounts in wild plants. Regarding the antioxidant activity, wild and cultivated plants were more effective in the oxidative haemolysis (OxHLIA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Moreover, both extracts showed moderate cytotoxicity in non-tumor cell lines (PLP2), while cultivated plants were more effective against cervical carcinoma (HeLa), breast carcinoma (MCF-7) and non-small lung cancer (NCI-H460) cell lines. Finally, wild plants showed higher antimicrobial activity than cultivated plants against specific pathogens. In conclusion, the cultivation of C. raphanina subsp. mixta showed promising results in terms of tocopherols content and antiproliferative effects, however further research is needed to decrease oxalic acid content.
PB  - MDPI AG
T2  - Antioxidants
T1  - Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds
IS  - 4
VL  - 9
DO  - 10.3390/antiox9040314
SP  - 314
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Dias, Maria Ines and Pereira, Carla and Calhelha, Ricardo and Di Gioia, Francesco and Tzortzakis, Nikolaos and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "Centaurea raphanina subsp. mixta (DC.) Runemark is a wild edible species endemic to Greece. This study evaluated the chemical composition and bioactive properties of wild and cultivated C. raphanina subsp. mixta plants. Wild plants had higher nutritional value than cultivated ones, whereas cultivated plants contained more tocopherols. Glucose and sucrose were higher in cultivated plants and trehalose in wild ones. Oxalic and total organic acids were detected in higher amounts in cultivated samples. The main fatty acids were α-linolenic, linoleic and palmitic acid, while wild plants were richer in polyunsaturated fatty acids. Two pinocembrin derivatives were the main phenolic compounds being detected in higher amounts in wild plants. Regarding the antioxidant activity, wild and cultivated plants were more effective in the oxidative haemolysis (OxHLIA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Moreover, both extracts showed moderate cytotoxicity in non-tumor cell lines (PLP2), while cultivated plants were more effective against cervical carcinoma (HeLa), breast carcinoma (MCF-7) and non-small lung cancer (NCI-H460) cell lines. Finally, wild plants showed higher antimicrobial activity than cultivated plants against specific pathogens. In conclusion, the cultivation of C. raphanina subsp. mixta showed promising results in terms of tocopherols content and antiproliferative effects, however further research is needed to decrease oxalic acid content.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds",
number = "4",
volume = "9",
doi = "10.3390/antiox9040314",
pages = "314"
}

Petropoulos, S. A., Fernandes, Â., Dias, M. I., Pereira, C., Calhelha, R., Di Gioia, F., Tzortzakis, N., Ivanov, M., Soković, M., Barros, L.,& Ferreira, I. C. F. R.. (2020). Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds. in Antioxidants
MDPI AG., 9(4), 314.
https://doi.org/10.3390/antiox9040314

Petropoulos SA, Fernandes Â, Dias MI, Pereira C, Calhelha R, Di Gioia F, Tzortzakis N, Ivanov M, Soković M, Barros L, Ferreira ICFR. Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds. in Antioxidants. 2020;9(4):314.
doi:10.3390/antiox9040314 .

Petropoulos, Spyridon A., Fernandes, Ângela, Dias, Maria Ines, Pereira, Carla, Calhelha, Ricardo, Di Gioia, Francesco, Tzortzakis, Nikolaos, Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C. F. R., "Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds" in Antioxidants, 9, no. 4 (2020):314,
https://doi.org/10.3390/antiox9040314 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Arampatzis, Dimitrios A.
AU  - Tsiropoulos, Nikolaos G.
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - internal-pdf://Petropoulos et al. - 2020 - Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.) A new insight to plant-based sou.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3602
AB  - In the present study, nutritional value, chemical composition and bioactive properties of purslane seeds, seed oils and seedcakes were examined. Data were analyzed by a one-way ANOVA, while means were compared with Tukey's HSD test. For seed oil extraction mechanical and ultrasound assisted methods were tested. Cold extraction methods (CE1 and CE2) resulted in higher oil yield (increased by 33.7% and 38.1%, respectively) comparing to hot extraction (HE) method. Seeds contained the highest amount of fats and energy (15.03 ± 0.06 g/100 g dry basis (db) and 459 ± 1 kcal/100 g db, respectively), while seedcakes from CE2 had the highest content in proteins and ash (31.20 ± 0.03 and 4.27 ± 0.06 g/100 g db, respectively). Seeds and seedcakes contained a balanced content of linoleic and α-linolenic acids (33.80–34.74% and 32.83–34.64%, respectively). HE and CE1 oils had slightly higher amounts of α-linolenic (39.67% and 39.57%, respectively) than linoleic acid (35.44% and 35.13%, respectively), whereas CE2 oils contained twice as much linoleic as α-linolenic acid (49.77% and 24.18%, respectively). In conclusion, the tested materials are good sources of omega-6 and omega-3 fatty acids and proteins, while extraction method affected oil yield and fatty acids composition of seed oils.
T2  - LWT
T1  - Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids
VL  - 123
DO  - 10.1016/j.lwt.2020.109099
SP  - 109099
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Arampatzis, Dimitrios A. and Tsiropoulos, Nikolaos G. and Petrović, Jovana and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "In the present study, nutritional value, chemical composition and bioactive properties of purslane seeds, seed oils and seedcakes were examined. Data were analyzed by a one-way ANOVA, while means were compared with Tukey's HSD test. For seed oil extraction mechanical and ultrasound assisted methods were tested. Cold extraction methods (CE1 and CE2) resulted in higher oil yield (increased by 33.7% and 38.1%, respectively) comparing to hot extraction (HE) method. Seeds contained the highest amount of fats and energy (15.03 ± 0.06 g/100 g dry basis (db) and 459 ± 1 kcal/100 g db, respectively), while seedcakes from CE2 had the highest content in proteins and ash (31.20 ± 0.03 and 4.27 ± 0.06 g/100 g db, respectively). Seeds and seedcakes contained a balanced content of linoleic and α-linolenic acids (33.80–34.74% and 32.83–34.64%, respectively). HE and CE1 oils had slightly higher amounts of α-linolenic (39.67% and 39.57%, respectively) than linoleic acid (35.44% and 35.13%, respectively), whereas CE2 oils contained twice as much linoleic as α-linolenic acid (49.77% and 24.18%, respectively). In conclusion, the tested materials are good sources of omega-6 and omega-3 fatty acids and proteins, while extraction method affected oil yield and fatty acids composition of seed oils.",
journal = "LWT",
title = "Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids",
volume = "123",
doi = "10.1016/j.lwt.2020.109099",
pages = "109099"
}

Petropoulos, S. A., Fernandes, Â., Arampatzis, D. A., Tsiropoulos, N. G., Petrović, J., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2020). Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids. in LWT, 123, 109099.
https://doi.org/10.1016/j.lwt.2020.109099

Petropoulos SA, Fernandes Â, Arampatzis DA, Tsiropoulos NG, Petrović J, Soković M, Barros L, Ferreira IC. Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids. in LWT. 2020;123:109099.
doi:10.1016/j.lwt.2020.109099 .

Petropoulos, Spyridon A., Fernandes, Ângela, Arampatzis, Dimitrios A., Tsiropoulos, Nikolaos G., Petrović, Jovana, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Seed oil and seed oil byproducts of common purslane (Portulaca oleracea L.): A new insight to plant-based sources rich in omega-3 fatty acids" in LWT, 123 (2020):109099,
https://doi.org/10.1016/j.lwt.2020.109099 . .

TY  - JOUR
AU  - Cruz, Luís R. O.
AU  - Polyzos, Nikolaos
AU  - Fernandes, Ângela
AU  - Petropoulos, Spyridon A.
AU  - Gioia, Francesco Di
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://www.mdpi.com/2073-4395/10/11/1824
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4093
AB  - The present study investigated the effect of salinity (Control: 1.8 dS/m, S1: 3.0 dS/m and S2: 4.5 dS/m) on the chemical composition and bioactive properties of three basil cultivars (Red Basil, Dark Opal Red and Basilico Rosso). Crop performance was not affected by increasing salinity in DoR and BaR. Fat, ash and carbohydrates content increased by salinity in DoR, whereas energetic value was negatively affected. Free sugars (total and individual compounds) increased under saline conditions (S2) in BaR, whereas reducing trends were observed for the main organic acids and tocopherols in all the cultivars. The major fatty acids were α-linolenic, linoleic and palmitic acids with no consistent salinity effects, while the richest polyphenols were sagerinic acid and eriodictyol-O-malonylhexoside. Finally, basil extracts showed moderate antioxidant and strong antifungal activity. In conclusion, salinity showed a genotype dependent effect on the chemical profile and bioactivities of the tested cultivars.
PB  - MDPI AG
T2  - Agronomy
T1  - Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars
IS  - 11
VL  - 10
DO  - 10.3390/agronomy10111824
SP  - 1824
ER  - 

@article{
author = "Cruz, Luís R. O. and Polyzos, Nikolaos and Fernandes, Ângela and Petropoulos, Spyridon A. and Gioia, Francesco Di and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2020",
abstract = "The present study investigated the effect of salinity (Control: 1.8 dS/m, S1: 3.0 dS/m and S2: 4.5 dS/m) on the chemical composition and bioactive properties of three basil cultivars (Red Basil, Dark Opal Red and Basilico Rosso). Crop performance was not affected by increasing salinity in DoR and BaR. Fat, ash and carbohydrates content increased by salinity in DoR, whereas energetic value was negatively affected. Free sugars (total and individual compounds) increased under saline conditions (S2) in BaR, whereas reducing trends were observed for the main organic acids and tocopherols in all the cultivars. The major fatty acids were α-linolenic, linoleic and palmitic acids with no consistent salinity effects, while the richest polyphenols were sagerinic acid and eriodictyol-O-malonylhexoside. Finally, basil extracts showed moderate antioxidant and strong antifungal activity. In conclusion, salinity showed a genotype dependent effect on the chemical profile and bioactivities of the tested cultivars.",
publisher = "MDPI AG",
journal = "Agronomy",
title = "Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars",
number = "11",
volume = "10",
doi = "10.3390/agronomy10111824",
pages = "1824"
}

Cruz, L. R. O., Polyzos, N., Fernandes, Â., Petropoulos, S. A., Gioia, F. D., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Ferreira, I. C. F. R.,& Barros, L.. (2020). Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars. in Agronomy
MDPI AG., 10(11), 1824.
https://doi.org/10.3390/agronomy10111824

Cruz LRO, Polyzos N, Fernandes Â, Petropoulos SA, Gioia FD, Dias MI, Pinela J, Kostić M, Soković M, Ferreira ICFR, Barros L. Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars. in Agronomy. 2020;10(11):1824.
doi:10.3390/agronomy10111824 .

Cruz, Luís R. O., Polyzos, Nikolaos, Fernandes, Ângela, Petropoulos, Spyridon A., Gioia, Francesco Di, Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, "Effect of Saline Conditions on Chemical Profile and the Bioactive Properties of Three Red-Colored Basil Cultivars" in Agronomy, 10, no. 11 (2020):1824,
https://doi.org/10.3390/agronomy10111824 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A
AU  - Taofiq, Oludemi
AU  - Fernandes, Ângela
AU  - Tzortzakis, Nikos
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel CFR
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.9881
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3445
AB  - BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.
T2  - Journal of the Science of Food and Agriculture
T1  - Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.
DO  - 10.1002/jsfa.9881
ER  - 

@article{
author = "Petropoulos, Spyridon A and Taofiq, Oludemi and Fernandes, Ângela and Tzortzakis, Nikos and Ćirić, Ana and Soković, Marina and Barros, Lillian and Ferreira, Isabel CFR",
year = "2019",
abstract = "BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.",
journal = "Journal of the Science of Food and Agriculture",
title = "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.",
doi = "10.1002/jsfa.9881"
}

Petropoulos, S. A., Taofiq, O., Fernandes, Â., Tzortzakis, N., Ćirić, A., Soković, M., Barros, L.,& Ferreira, I. C.. (2019). Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture.
https://doi.org/10.1002/jsfa.9881

Petropoulos SA, Taofiq O, Fernandes Â, Tzortzakis N, Ćirić A, Soković M, Barros L, Ferreira IC. Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture. 2019;.
doi:10.1002/jsfa.9881 .

Petropoulos, Spyridon A, Taofiq, Oludemi, Fernandes, Ângela, Tzortzakis, Nikos, Ćirić, Ana, Soković, Marina, Barros, Lillian, Ferreira, Isabel CFR, "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect." in Journal of the Science of Food and Agriculture (2019),
https://doi.org/10.1002/jsfa.9881 . .

TY  - JOUR
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Petropoulos, Spyridon A.
AU  - Plexida, Sofia
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikos
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - C F R Ferreira, Isabel
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4494
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3577
AB  - In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244494
SP  - 4494
ER  - 

@article{
author = "Pereira, Carla and Dias, Maria Inês and Petropoulos, Spyridon A. and Plexida, Sofia and Chrysargyris, Antonios and Tzortzakis, Nikos and Calhelha, Ricardo C. and Ivanov, Marija and Stojković, Dejan and Soković, Marina and Barros, Lillian and C F R Ferreira, Isabel",
year = "2019",
abstract = "In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).",
number = "24",
volume = "24",
doi = "10.3390/molecules24244494",
pages = "4494"
}

Pereira, C., Dias, M. I., Petropoulos, S. A., Plexida, S., Chrysargyris, A., Tzortzakis, N., Calhelha, R. C., Ivanov, M., Stojković, D., Soković, M., Barros, L.,& C F R Ferreira, I.. (2019). The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4494.
https://doi.org/10.3390/molecules24244494

Pereira C, Dias MI, Petropoulos SA, Plexida S, Chrysargyris A, Tzortzakis N, Calhelha RC, Ivanov M, Stojković D, Soković M, Barros L, C F R Ferreira I. The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland). 2019;24(24):4494.
doi:10.3390/molecules24244494 .

Pereira, Carla, Dias, Maria Inês, Petropoulos, Spyridon A., Plexida, Sofia, Chrysargyris, Antonios, Tzortzakis, Nikos, Calhelha, Ricardo C., Ivanov, Marija, Stojković, Dejan, Soković, Marina, Barros, Lillian, C F R Ferreira, Isabel, "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4494,
https://doi.org/10.3390/molecules24244494 . .

TY  - JOUR
AU  - Petropoulos, Spyridon A.
AU  - Fernandes, Ângela
AU  - Tzortzakis, Nikolaos
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0963996918308615?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3169
AB  - Nine wild edible species belonging to Astreaceae family, native to the Mediterranean basin were tested for their chemical composition (phenolic compounds, tocopherols, and organic acids) and antimicrobial activities over two growing periods, apart from Scolymus hispanicus and Hedypnois cretica which were tested for only one growing period. Flavonoids were the most abundant phenolic compounds in all the species, except for the case of Taraxacum species where significant amounts of chicoric acid were detected, while phenolic compounds content increased in the 2nd growing period by 4.6–397.4% for the tested species. α- and β-tocopherols were the main tocopherols, apart from Taraxacum sp. where significant amounts of γ-and δ-tocopherols (18.32 and 16.31 μg/100 g fresh weight) were detected, while total tocopherols content either increased (Reicardia picroides, Picris echioides, Urospermum picroides, and Taraxacum officinale) or decreased (Hymenonema graecum, Sonchus oleraceus, Taraxacum sp.) in the 2nd growing period. Oxalic acid was the most abundant organic acid, with the highest content (972 mg/100 g fresh weight) being observed in H. graecum (L.) DC. in the 1st growing period. Moreover, with the exception of H. graecum and S. olearaceus, total organic acids content increased in the 2nd growing period. Significant antimicrobial activities were observed against Bacillus cereus, Salmonella typhimurium and Penicillium ochrochloron for all the studied species. In conclusion, the studied species showed great potential for commercial cultivation, while plant extracts could find use in the food industry as alternative food preservatives.
T2  - Food Research International
T1  - Bioactive compounds content and antimicrobial activities of wild edible Asteraceae species of the Mediterranean flora under commercial cultivation conditions
VL  - 119
DO  - 10.1016/J.FOODRES.2018.10.069
SP  - 859
EP  - 868
ER  - 

@article{
author = "Petropoulos, Spyridon A. and Fernandes, Ângela and Tzortzakis, Nikolaos and Soković, Marina and Ćirić, Ana and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2019",
abstract = "Nine wild edible species belonging to Astreaceae family, native to the Mediterranean basin were tested for their chemical composition (phenolic compounds, tocopherols, and organic acids) and antimicrobial activities over two growing periods, apart from Scolymus hispanicus and Hedypnois cretica which were tested for only one growing period. Flavonoids were the most abundant phenolic compounds in all the species, except for the case of Taraxacum species where significant amounts of chicoric acid were detected, while phenolic compounds content increased in the 2nd growing period by 4.6–397.4% for the tested species. α- and β-tocopherols were the main tocopherols, apart from Taraxacum sp. where significant amounts of γ-and δ-tocopherols (18.32 and 16.31 μg/100 g fresh weight) were detected, while total tocopherols content either increased (Reicardia picroides, Picris echioides, Urospermum picroides, and Taraxacum officinale) or decreased (Hymenonema graecum, Sonchus oleraceus, Taraxacum sp.) in the 2nd growing period. Oxalic acid was the most abundant organic acid, with the highest content (972 mg/100 g fresh weight) being observed in H. graecum (L.) DC. in the 1st growing period. Moreover, with the exception of H. graecum and S. olearaceus, total organic acids content increased in the 2nd growing period. Significant antimicrobial activities were observed against Bacillus cereus, Salmonella typhimurium and Penicillium ochrochloron for all the studied species. In conclusion, the studied species showed great potential for commercial cultivation, while plant extracts could find use in the food industry as alternative food preservatives.",
journal = "Food Research International",
title = "Bioactive compounds content and antimicrobial activities of wild edible Asteraceae species of the Mediterranean flora under commercial cultivation conditions",
volume = "119",
doi = "10.1016/J.FOODRES.2018.10.069",
pages = "859-868"
}

Petropoulos, S. A., Fernandes, Â., Tzortzakis, N., Soković, M., Ćirić, A., Barros, L.,& Ferreira, I. C.F.R.. (2019). Bioactive compounds content and antimicrobial activities of wild edible Asteraceae species of the Mediterranean flora under commercial cultivation conditions. in Food Research International, 119, 859-868.
https://doi.org/10.1016/J.FOODRES.2018.10.069

Petropoulos SA, Fernandes Â, Tzortzakis N, Soković M, Ćirić A, Barros L, Ferreira IC. Bioactive compounds content and antimicrobial activities of wild edible Asteraceae species of the Mediterranean flora under commercial cultivation conditions. in Food Research International. 2019;119:859-868.
doi:10.1016/J.FOODRES.2018.10.069 .

Petropoulos, Spyridon A., Fernandes, Ângela, Tzortzakis, Nikolaos, Soković, Marina, Ćirić, Ana, Barros, Lillian, Ferreira, Isabel C.F.R., "Bioactive compounds content and antimicrobial activities of wild edible Asteraceae species of the Mediterranean flora under commercial cultivation conditions" in Food Research International, 119 (2019):859-868,
https://doi.org/10.1016/J.FOODRES.2018.10.069 . .