TY  - GEN
AU  - Mojić, Marija
AU  - Ucche, Sisca
AU  - Yokoyama, Satoru
AU  - Hayakawa, Yoshihiro
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6263
AB  - IFNγ је кључни цитокин антитуморског имунског одговора. У ћелијама канцера, IFNγ стимулише производњу реактивних врста кисеоника. Настало стање
оксидативног стреса доводи до оштећења ДНК и, последично, до сенесценције
или до фероптозе, ћелијске смрти посредоване пероксидацијом липида. Неадекватан одговор на IFNγ је честа одлика канцера са способних да „побегну“
антитуморском имунском одговору. Уједно, овај дефект у одговору на IFNγ се
сматра значајним чиниоцем ограниченог успеха имунотерапије код пацијанта
са канцером.
У овој студији, истражили смо како ћелије тумора избегавају антитуморски
имунски одговор посредован цитокином IFNγ. Успоставили смо ћелијске линије меланома које су „побегле“ антитуморском имунском одговору и анализирали смо промене у њиховом фенотипу. Открили смо да су ћелије меланома
које су избегле имунски одговор развиле резистенцију на оксидативни стрес
индукован IFNγ. Кључни играч у овом процесу је глутатион-С-трансфераза
(енг.GSTA4), члан породице ензима за детоксикацију који имају важну улогу у
ћелијском одговору на оксидативни стрес. Осим тога, ћелије меланома које су
избегле имунски одговор су стекле већи метастатски потенцијал in vivo, такође
зависан од повећаног нивоа експресије GSTA4. Код пацијената са меланомом
утврђено је да нижи ниво експресије GSTA4 корелира са бољом стопом преживљавања без метастаза. Такође, пацијенти са меланомом код којих је нижа
GSTA4 екпресија боље реагују на анти-PD1 терапију и имају су бољу стопу преживљавања без прогресије тумора.
Наши резултати расветљавају нови механизам помоћу којег ћелије канцера избегавају имунолошки надзор и повећавају свој метастатски потенцијал, развијањем резистенције на оксидативни стрес услед повећане експресије GSTA4. Стога,
таргетирање молекула у одговору канцера на оксидативни стрес представља обећавајући терапеутски приступ за превазилажење резистенције на антитуморски
имунски одговор и регулисање метастазирања.
AB  - IFNγ is a crucial cytokine in antitumor immunity. In cancer cells, IFNγ promotes excessive
production of the reactive oxygen species. Oxidative stress leads to DNA damage
and, consequently, triggers cellular senescence or ferroptosis, a type of cell death associated
with increased lipid peroxidation. The IFNγ response defect is commonly observed in
cancers that exhibit immunoevasive properties. This defect is considered a significant factor
contributing to the limited success of cancer immunotherapy in patients with cancer.
In this study, we explored how tumor cells evade the IFNγ-dependent immune response.
We established immune-escape variants of melanoma cells and analyzed changes in their
phenotype. We found that the immune-escape melanoma variants gained resistance to
the IFNγ-induced oxidative stress response. The critical molecule in this process was glutathione-
S-transferase-4 (GSTA4), a member of a family of detoxification enzymes that
play an important role in cellular oxidative stress responses. In addition to the resistance
to IFNγ-mediated antitumor immunity, the immune-escape melanoma variants acquired
higher metastatic ability in vivo by a GSTA4-dependent mechanism. Melanoma patients
with lower expression of GSTA4 had better prognosis in terms of metastasis-free survival
rate. Additionally, melanoma patients with low GSTA4 expression were better responders
to anti-PD1 therapy and showed a better progression-free survival rate.
Our results reveal a novel mechanism by which cancer cells escape from immune
surveillance and increase metastatic potential by developing resistance to oxidative
stress responses through GSTA4 upregulation. Therefore, targeting the oxidative stress
response in cancer cells presents a promising therapeutic approach for overcoming immune
resistance and regulating metastatic progression.
PB  - Kragujevac: Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
T2  - Abstract Book: The second Serbian Molecular Medicine Congress; 2023 Oct 6-8; Foča, Bosnia and Herzegovina
T1  - Повећана експресија GSTA4 у меланому подстиче избегавање антитуморског имунског одговора и повећава метастатски потенцијал
T1  - GSTA4 upregulation promotes melanoma immune evasion and metastasis
SP  - 53
EP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6263
ER  - 

@misc{
author = "Mojić, Marija and Ucche, Sisca and Yokoyama, Satoru and Hayakawa, Yoshihiro",
year = "2023",
abstract = "IFNγ је кључни цитокин антитуморског имунског одговора. У ћелијама канцера, IFNγ стимулише производњу реактивних врста кисеоника. Настало стање
оксидативног стреса доводи до оштећења ДНК и, последично, до сенесценције
или до фероптозе, ћелијске смрти посредоване пероксидацијом липида. Неадекватан одговор на IFNγ је честа одлика канцера са способних да „побегну“
антитуморском имунском одговору. Уједно, овај дефект у одговору на IFNγ се
сматра значајним чиниоцем ограниченог успеха имунотерапије код пацијанта
са канцером.
У овој студији, истражили смо како ћелије тумора избегавају антитуморски
имунски одговор посредован цитокином IFNγ. Успоставили смо ћелијске линије меланома које су „побегле“ антитуморском имунском одговору и анализирали смо промене у њиховом фенотипу. Открили смо да су ћелије меланома
које су избегле имунски одговор развиле резистенцију на оксидативни стрес
индукован IFNγ. Кључни играч у овом процесу је глутатион-С-трансфераза
(енг.GSTA4), члан породице ензима за детоксикацију који имају важну улогу у
ћелијском одговору на оксидативни стрес. Осим тога, ћелије меланома које су
избегле имунски одговор су стекле већи метастатски потенцијал in vivo, такође
зависан од повећаног нивоа експресије GSTA4. Код пацијената са меланомом
утврђено је да нижи ниво експресије GSTA4 корелира са бољом стопом преживљавања без метастаза. Такође, пацијенти са меланомом код којих је нижа
GSTA4 екпресија боље реагују на анти-PD1 терапију и имају су бољу стопу преживљавања без прогресије тумора.
Наши резултати расветљавају нови механизам помоћу којег ћелије канцера избегавају имунолошки надзор и повећавају свој метастатски потенцијал, развијањем резистенције на оксидативни стрес услед повећане експресије GSTA4. Стога,
таргетирање молекула у одговору канцера на оксидативни стрес представља обећавајући терапеутски приступ за превазилажење резистенције на антитуморски
имунски одговор и регулисање метастазирања., IFNγ is a crucial cytokine in antitumor immunity. In cancer cells, IFNγ promotes excessive
production of the reactive oxygen species. Oxidative stress leads to DNA damage
and, consequently, triggers cellular senescence or ferroptosis, a type of cell death associated
with increased lipid peroxidation. The IFNγ response defect is commonly observed in
cancers that exhibit immunoevasive properties. This defect is considered a significant factor
contributing to the limited success of cancer immunotherapy in patients with cancer.
In this study, we explored how tumor cells evade the IFNγ-dependent immune response.
We established immune-escape variants of melanoma cells and analyzed changes in their
phenotype. We found that the immune-escape melanoma variants gained resistance to
the IFNγ-induced oxidative stress response. The critical molecule in this process was glutathione-
S-transferase-4 (GSTA4), a member of a family of detoxification enzymes that
play an important role in cellular oxidative stress responses. In addition to the resistance
to IFNγ-mediated antitumor immunity, the immune-escape melanoma variants acquired
higher metastatic ability in vivo by a GSTA4-dependent mechanism. Melanoma patients
with lower expression of GSTA4 had better prognosis in terms of metastasis-free survival
rate. Additionally, melanoma patients with low GSTA4 expression were better responders
to anti-PD1 therapy and showed a better progression-free survival rate.
Our results reveal a novel mechanism by which cancer cells escape from immune
surveillance and increase metastatic potential by developing resistance to oxidative
stress responses through GSTA4 upregulation. Therefore, targeting the oxidative stress
response in cancer cells presents a promising therapeutic approach for overcoming immune
resistance and regulating metastatic progression.",
publisher = "Kragujevac: Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine",
journal = "Abstract Book: The second Serbian Molecular Medicine Congress; 2023 Oct 6-8; Foča, Bosnia and Herzegovina",
title = "Повећана експресија GSTA4 у меланому подстиче избегавање антитуморског имунског одговора и повећава метастатски потенцијал, GSTA4 upregulation promotes melanoma immune evasion and metastasis",
pages = "53-55",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6263"
}

Mojić, M., Ucche, S., Yokoyama, S.,& Hayakawa, Y.. (2023). Повећана експресија GSTA4 у меланому подстиче избегавање антитуморског имунског одговора и повећава метастатски потенцијал. in Abstract Book: The second Serbian Molecular Medicine Congress; 2023 Oct 6-8; Foča, Bosnia and Herzegovina
Kragujevac: Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine., 53-55.
https://hdl.handle.net/21.15107/rcub_ibiss_6263

Mojić M, Ucche S, Yokoyama S, Hayakawa Y. Повећана експресија GSTA4 у меланому подстиче избегавање антитуморског имунског одговора и повећава метастатски потенцијал. in Abstract Book: The second Serbian Molecular Medicine Congress; 2023 Oct 6-8; Foča, Bosnia and Herzegovina. 2023;:53-55.
https://hdl.handle.net/21.15107/rcub_ibiss_6263 .

Mojić, Marija, Ucche, Sisca, Yokoyama, Satoru, Hayakawa, Yoshihiro, "Повећана експресија GSTA4 у меланому подстиче избегавање антитуморског имунског одговора и повећава метастатски потенцијал" in Abstract Book: The second Serbian Molecular Medicine Congress; 2023 Oct 6-8; Foča, Bosnia and Herzegovina (2023):53-55,
https://hdl.handle.net/21.15107/rcub_ibiss_6263 .

TY  - GEN
AU  - Mojić, Marija
AU  - Ucche, Sisca
AU  - Yokoyama, Satoru
AU  - Yoshihiro, Hayakawa
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6264
AB  - Background: Interferon-gamma (IFNγ) is a crucial eff ector molecule of antitumor immunity. This cytokine promotes the excessive production of reactive oxygen species (ROS) in tumor cells, which leads to DNA damage and senescence [1]. Recently it has been discovered that IFNγ can also trigger cancer cell ferroptosis by fostering lipid peroxidation [2]. Cancers often evade antitumor immunity by losing their responsiveness to IFNγ. Consequently, IFNγ becomes a critical player in the immunoediting process, selecting tumor cells with immunoevasive properties [3]. Defects in responsiveness to IFNγ in cancer cells significantly contribute to the limited success of cancer immunotherapy in clinics [4], emphasizing the importance of understanding the mechanism behind the IFNγ-mediated immunoeding process. To
address this issue, we investigated how tumor cells escape IFNγ-dependent immune response through immunoediting by analyzing originally established immune-escape variants of melanoma cells. Material and Methods: We used a previously established in vivo model in which antitumor immunity was IFNγ dependent [5]. Mouse B16 melanoma cells expressing ovalbumin as a tumor-specific antigen (B16OVA) were subcutaneously inoculated in OVA-immunized B6 mice. In this model, tumor growth suppression by host IFN-γ lasts for a limited time, after which all tumors progress. Next, we established cancer cell lines with different in vivo immunological experiences. Tumor cells were isolated from same-sized tumors from wild-type (WT) untreated mice (established cell lines were named "NIMM"), from WT OVAimmunized
mice after the cessation of immune control of tumor growth (established cell lines were named "IMM"), or
from IFNγ knockout (IFNγ KO) OVA-immunized mice (established cell lines were named "GKO-IMM"). IMM, NIMM, and GKO-IMM cells were re-challenged in OVA-immunized mice to test their ability to provoke antitumor immunity. Instead of immunization with OVA antigen, in some experiments, the anti-PD-1 antibody was administered intraperitoneally to initiate tumor-specific immunity in vivo. To examine changes in phenotype resulting from the IFNγ immunoediting process, total RNA was extracted from parental B16OVA cells and immune-escaped IMM cells. Gene expression was analyzed using a GeneChip system with GeneChip Mouse Gene 2.0 ST Array. mRNA and protein expression of selected genes was quantitatively determined by real-time PCR and western blotting, respectively. GSTA4 overexpression or knockdown was performed to determine its functional role in the immunoevasive phenotype of IMM cells. Cell sensitivity to IFNγ and 4–hydroxynonenal (4–HNE), a lipid peroxidation product, was estimated by WST–8 cell viability assay. CellROX Deep Red reagent was used to detect IFNγ-induced intracellular ROS accumulation. Transwell invasion assay was used
to assess melanoma cells' in vitro metastatic potential. In the in vivo experimental lung metastasis model, cells were injected into the tail vein and metastasized tumor colonies on the surface of the lungs were counted. The correlation of GSTA4 expression in human melanoma patients with tumor-free survival rates, and response to anti–PD1 treatment in correlation with GSTA4 expression and survival rates were obtained from publicly available databases. Results: Upon re-challenging into OVA-immunized mice, IMM cells showed unrestrained progression, while the growth of NIMM and GKO-IMM tumors was suppressed. In addition, only IMM cells specifically lost OVA antigen expression, indicating that these cells gained the ability to evade the OVA-specific antitumor immune response. In line with in vivo data, IFNγ treatment in vitro reduced the viability of parental B16OVA, NIMM, and GKO-IMM cells, while the viability of IMM cells was intact. Interestingly, IFNγ upregulated the expression of MHC class I (H-2Kd) and PD-L1in IMM cells,
suggesting that these cells did not have the defect in IFNγ signaling. We found that the lack of IMM cell responsiveness to the IFNγ-induced cytostatic effect was due to the acquisition of resistance to the IFNγ-induced oxidative stress response. Gene expression analysis using DNA microarray revealed that the most upregulated gene in immunoevasive IMM cells was glutathione-S-transferase-4 (GSTA4). GSTA4 is a member of a family of detoxification enzymes that play an essential protective role in cellular oxidative stress responses [6]. GSTA4 overexpression in parental B16OVA cells reduced ROS production and increased their resistance to the IFNγ-induced cytostatic effect in vitro. Consequently, the growth of B16OVA cells overexpressing GSTA4 was more aggressive in OVA-immunized mice than that of parental
B16OVA cells. In parallel, the knockdown of GSTA4 in IMM cells led to increased intracellular ROS levels and decreased viability upon in vitro IFNγ treatment. IMM tumors were resistant to anti-PD1 treatment in vivo, and the knockdown of GSTA4 reinvigorated their responsiveness. In addition to the role in acquired resistance to IFNγ, we found that the upregulation of GSTA4 was also responsible for the higher metastatic potential of IMM tumors. Next, we confirmed the results from the mouse model in human melanoma. GSTA4 expression levels in Malme3M, UACC 62, and MeWo melanoma cell lines inversely correlated with their sensitivity to in vitro IFNγ treatment. Database analysis revealed a significant correlation between the expression of GSTA4 and the metastasis-free survival rate of human melanoma
patients. Melanoma patients with low GSTA4 expression were better responders and showed a better progression-free survival rate to anti-PD1 therapy, further supporting the clinical relevance of our findings. Conclusion: In this study, we uncovered a new mechanism through which cancer cells evade immune surveillance and enhance their ability to metastasize by developing resistance to oxidative stress responses through GSTA4 upregulation. Our results suggest that targeting the oxidative stress response in cancer cells emerges as a promising therapeutic strategy to overcome immune resistance and regulate the progression of metastasis [7].
PB  - Belgrade: Serbian Association for Cancer Research
T2  - Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
T1  - Control of IFN-γ Responsiveness and Metastatic Potential in Melanoma by GSTA4
SP  - 50
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6264
ER  - 

@misc{
author = "Mojić, Marija and Ucche, Sisca and Yokoyama, Satoru and Yoshihiro, Hayakawa",
year = "2023",
abstract = "Background: Interferon-gamma (IFNγ) is a crucial eff ector molecule of antitumor immunity. This cytokine promotes the excessive production of reactive oxygen species (ROS) in tumor cells, which leads to DNA damage and senescence [1]. Recently it has been discovered that IFNγ can also trigger cancer cell ferroptosis by fostering lipid peroxidation [2]. Cancers often evade antitumor immunity by losing their responsiveness to IFNγ. Consequently, IFNγ becomes a critical player in the immunoediting process, selecting tumor cells with immunoevasive properties [3]. Defects in responsiveness to IFNγ in cancer cells significantly contribute to the limited success of cancer immunotherapy in clinics [4], emphasizing the importance of understanding the mechanism behind the IFNγ-mediated immunoeding process. To
address this issue, we investigated how tumor cells escape IFNγ-dependent immune response through immunoediting by analyzing originally established immune-escape variants of melanoma cells. Material and Methods: We used a previously established in vivo model in which antitumor immunity was IFNγ dependent [5]. Mouse B16 melanoma cells expressing ovalbumin as a tumor-specific antigen (B16OVA) were subcutaneously inoculated in OVA-immunized B6 mice. In this model, tumor growth suppression by host IFN-γ lasts for a limited time, after which all tumors progress. Next, we established cancer cell lines with different in vivo immunological experiences. Tumor cells were isolated from same-sized tumors from wild-type (WT) untreated mice (established cell lines were named "NIMM"), from WT OVAimmunized
mice after the cessation of immune control of tumor growth (established cell lines were named "IMM"), or
from IFNγ knockout (IFNγ KO) OVA-immunized mice (established cell lines were named "GKO-IMM"). IMM, NIMM, and GKO-IMM cells were re-challenged in OVA-immunized mice to test their ability to provoke antitumor immunity. Instead of immunization with OVA antigen, in some experiments, the anti-PD-1 antibody was administered intraperitoneally to initiate tumor-specific immunity in vivo. To examine changes in phenotype resulting from the IFNγ immunoediting process, total RNA was extracted from parental B16OVA cells and immune-escaped IMM cells. Gene expression was analyzed using a GeneChip system with GeneChip Mouse Gene 2.0 ST Array. mRNA and protein expression of selected genes was quantitatively determined by real-time PCR and western blotting, respectively. GSTA4 overexpression or knockdown was performed to determine its functional role in the immunoevasive phenotype of IMM cells. Cell sensitivity to IFNγ and 4–hydroxynonenal (4–HNE), a lipid peroxidation product, was estimated by WST–8 cell viability assay. CellROX Deep Red reagent was used to detect IFNγ-induced intracellular ROS accumulation. Transwell invasion assay was used
to assess melanoma cells' in vitro metastatic potential. In the in vivo experimental lung metastasis model, cells were injected into the tail vein and metastasized tumor colonies on the surface of the lungs were counted. The correlation of GSTA4 expression in human melanoma patients with tumor-free survival rates, and response to anti–PD1 treatment in correlation with GSTA4 expression and survival rates were obtained from publicly available databases. Results: Upon re-challenging into OVA-immunized mice, IMM cells showed unrestrained progression, while the growth of NIMM and GKO-IMM tumors was suppressed. In addition, only IMM cells specifically lost OVA antigen expression, indicating that these cells gained the ability to evade the OVA-specific antitumor immune response. In line with in vivo data, IFNγ treatment in vitro reduced the viability of parental B16OVA, NIMM, and GKO-IMM cells, while the viability of IMM cells was intact. Interestingly, IFNγ upregulated the expression of MHC class I (H-2Kd) and PD-L1in IMM cells,
suggesting that these cells did not have the defect in IFNγ signaling. We found that the lack of IMM cell responsiveness to the IFNγ-induced cytostatic effect was due to the acquisition of resistance to the IFNγ-induced oxidative stress response. Gene expression analysis using DNA microarray revealed that the most upregulated gene in immunoevasive IMM cells was glutathione-S-transferase-4 (GSTA4). GSTA4 is a member of a family of detoxification enzymes that play an essential protective role in cellular oxidative stress responses [6]. GSTA4 overexpression in parental B16OVA cells reduced ROS production and increased their resistance to the IFNγ-induced cytostatic effect in vitro. Consequently, the growth of B16OVA cells overexpressing GSTA4 was more aggressive in OVA-immunized mice than that of parental
B16OVA cells. In parallel, the knockdown of GSTA4 in IMM cells led to increased intracellular ROS levels and decreased viability upon in vitro IFNγ treatment. IMM tumors were resistant to anti-PD1 treatment in vivo, and the knockdown of GSTA4 reinvigorated their responsiveness. In addition to the role in acquired resistance to IFNγ, we found that the upregulation of GSTA4 was also responsible for the higher metastatic potential of IMM tumors. Next, we confirmed the results from the mouse model in human melanoma. GSTA4 expression levels in Malme3M, UACC 62, and MeWo melanoma cell lines inversely correlated with their sensitivity to in vitro IFNγ treatment. Database analysis revealed a significant correlation between the expression of GSTA4 and the metastasis-free survival rate of human melanoma
patients. Melanoma patients with low GSTA4 expression were better responders and showed a better progression-free survival rate to anti-PD1 therapy, further supporting the clinical relevance of our findings. Conclusion: In this study, we uncovered a new mechanism through which cancer cells evade immune surveillance and enhance their ability to metastasize by developing resistance to oxidative stress responses through GSTA4 upregulation. Our results suggest that targeting the oxidative stress response in cancer cells emerges as a promising therapeutic strategy to overcome immune resistance and regulate the progression of metastasis [7].",
publisher = "Belgrade: Serbian Association for Cancer Research",
journal = "Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia",
title = "Control of IFN-γ Responsiveness and Metastatic Potential in Melanoma by GSTA4",
pages = "50-51",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6264"
}

Mojić, M., Ucche, S., Yokoyama, S.,& Yoshihiro, H.. (2023). Control of IFN-γ Responsiveness and Metastatic Potential in Melanoma by GSTA4. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
Belgrade: Serbian Association for Cancer Research., 50-51.
https://hdl.handle.net/21.15107/rcub_ibiss_6264

Mojić M, Ucche S, Yokoyama S, Yoshihiro H. Control of IFN-γ Responsiveness and Metastatic Potential in Melanoma by GSTA4. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia. 2023;:50-51.
https://hdl.handle.net/21.15107/rcub_ibiss_6264 .

Mojić, Marija, Ucche, Sisca, Yokoyama, Satoru, Yoshihiro, Hayakawa, "Control of IFN-γ Responsiveness and Metastatic Potential in Melanoma by GSTA4" in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia (2023):50-51,
https://hdl.handle.net/21.15107/rcub_ibiss_6264 .

TY  - JOUR
AU  - Useini, Liridona
AU  - Mojić, Marija
AU  - Laube, Markus
AU  - Lönnecke, Peter
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Pietzsch, Jens
AU  - Hey‐Hawkins, Evamarie
PY  - 2023
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cmdc.202200583
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5432
AB  - Fenoprofen is a widely used nonsteroidal anti-inflammatory drug (NSAID) against rheumatoid arthritis, degenerative joint disease, ankylosing spondylitis and gout. Like other NSAIDs, fenoprofen inhibits the synthesis of prostaglandins by blocking both cyclooxygenase (COX) isoforms, COX-1 the “house-keeping” enzyme and COX-2 the induced isoform from pathological stimuli. Unselective inhibition of both COX isoforms results in many side effects, but off-target effects have also been reported. The steric modifications of the drugs could afford the desired COX-2 selectivity. Furthermore, NSAIDs have shown promising cytotoxic properties. The structural modification of fenoprofen using bulky dicarba-closo-dodecaborane(12) (carborane) clusters and the biological evaluation of the carborane analogues for COX inhibition and antitumor potential showed that the carborane analogues exhibit stronger antitumor potential compared to their respective aryl-based compounds.
PB  - John Wiley and Sons Ltd
T2  - ChemMedChem
T1  - Carborane Analogues of Fenoprofen Exhibit Improved Antitumor Activity
IS  - 5
VL  - 18
DO  - 10.1002/cmdc.202200583
SP  - e202200583
ER  - 

@article{
author = "Useini, Liridona and Mojić, Marija and Laube, Markus and Lönnecke, Peter and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Pietzsch, Jens and Hey‐Hawkins, Evamarie",
year = "2023",
abstract = "Fenoprofen is a widely used nonsteroidal anti-inflammatory drug (NSAID) against rheumatoid arthritis, degenerative joint disease, ankylosing spondylitis and gout. Like other NSAIDs, fenoprofen inhibits the synthesis of prostaglandins by blocking both cyclooxygenase (COX) isoforms, COX-1 the “house-keeping” enzyme and COX-2 the induced isoform from pathological stimuli. Unselective inhibition of both COX isoforms results in many side effects, but off-target effects have also been reported. The steric modifications of the drugs could afford the desired COX-2 selectivity. Furthermore, NSAIDs have shown promising cytotoxic properties. The structural modification of fenoprofen using bulky dicarba-closo-dodecaborane(12) (carborane) clusters and the biological evaluation of the carborane analogues for COX inhibition and antitumor potential showed that the carborane analogues exhibit stronger antitumor potential compared to their respective aryl-based compounds.",
publisher = "John Wiley and Sons Ltd",
journal = "ChemMedChem",
title = "Carborane Analogues of Fenoprofen Exhibit Improved Antitumor Activity",
number = "5",
volume = "18",
doi = "10.1002/cmdc.202200583",
pages = "e202200583"
}

Useini, L., Mojić, M., Laube, M., Lönnecke, P., Mijatović, S., Maksimović-Ivanić, D., Pietzsch, J.,& Hey‐Hawkins, E.. (2023). Carborane Analogues of Fenoprofen Exhibit Improved Antitumor Activity. in ChemMedChem
John Wiley and Sons Ltd., 18(5), e202200583.
https://doi.org/10.1002/cmdc.202200583

Useini L, Mojić M, Laube M, Lönnecke P, Mijatović S, Maksimović-Ivanić D, Pietzsch J, Hey‐Hawkins E. Carborane Analogues of Fenoprofen Exhibit Improved Antitumor Activity. in ChemMedChem. 2023;18(5):e202200583.
doi:10.1002/cmdc.202200583 .

Useini, Liridona, Mojić, Marija, Laube, Markus, Lönnecke, Peter, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Pietzsch, Jens, Hey‐Hawkins, Evamarie, "Carborane Analogues of Fenoprofen Exhibit Improved Antitumor Activity" in ChemMedChem, 18, no. 5 (2023):e202200583,
https://doi.org/10.1002/cmdc.202200583 . .

TY  - JOUR
AU  - Markelić, Milica
AU  - Mojić, Marija
AU  - Bovan, Dijana
AU  - Jelača, Sanja
AU  - Jović, Zorana
AU  - Purić, Milica
AU  - Koruga, Djuro
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5470
AB  - In our recent study, we showed that in vitro treatment of melanoma cells with hyperpolarized light (HPL) as well as with the second derivative of fullerene, hyper-harmonized hydroxylated fullerene water complex (3HFWC) reduced viability of cells by decreasing their proliferative capacity and inducing senescence and reprogramming towards a normal, melanocytic phenotype. Therefore, we wanted to determine whether these effects persisted in vivo in the syngeneic mouse melanoma model with a combined treatment of HPL irradiation and 3HFWC per os. Our results demonstrated the potent antitumor effects of 3HFWC nanosubstance assisted by HPL irradiation. These effects were primarily driven by the stimulation of melanoma cell growth arrest, the establishment of a senescent phenotype, and melanocytic differentiation on the one hand, and the awakening of the antitumor immune response on the other. In addition, the combined treatment reduced the protumorigenic activity of immune cells by depleting T regulatory cells, myeloid-derived suppressors, and M2 macrophages. The support of the 3HFWC substance by HPL irradiation may be the axis of the new approach design based on tumor cell reprogramming synchronized with the mobilization of the host’s protective immune response.
PB  - Basel: MDPI
T2  - Nanomaterials
T1  - Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo
IS  - 3
VL  - 13
DO  - 10.3390/nano13030372
SP  - 372
ER  - 

@article{
author = "Markelić, Milica and Mojić, Marija and Bovan, Dijana and Jelača, Sanja and Jović, Zorana and Purić, Milica and Koruga, Djuro and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2023",
abstract = "In our recent study, we showed that in vitro treatment of melanoma cells with hyperpolarized light (HPL) as well as with the second derivative of fullerene, hyper-harmonized hydroxylated fullerene water complex (3HFWC) reduced viability of cells by decreasing their proliferative capacity and inducing senescence and reprogramming towards a normal, melanocytic phenotype. Therefore, we wanted to determine whether these effects persisted in vivo in the syngeneic mouse melanoma model with a combined treatment of HPL irradiation and 3HFWC per os. Our results demonstrated the potent antitumor effects of 3HFWC nanosubstance assisted by HPL irradiation. These effects were primarily driven by the stimulation of melanoma cell growth arrest, the establishment of a senescent phenotype, and melanocytic differentiation on the one hand, and the awakening of the antitumor immune response on the other. In addition, the combined treatment reduced the protumorigenic activity of immune cells by depleting T regulatory cells, myeloid-derived suppressors, and M2 macrophages. The support of the 3HFWC substance by HPL irradiation may be the axis of the new approach design based on tumor cell reprogramming synchronized with the mobilization of the host’s protective immune response.",
publisher = "Basel: MDPI",
journal = "Nanomaterials",
title = "Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo",
number = "3",
volume = "13",
doi = "10.3390/nano13030372",
pages = "372"
}

Markelić, M., Mojić, M., Bovan, D., Jelača, S., Jović, Z., Purić, M., Koruga, D., Mijatović, S.,& Maksimović-Ivanić, D.. (2023). Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo. in Nanomaterials
Basel: MDPI., 13(3), 372.
https://doi.org/10.3390/nano13030372

Markelić M, Mojić M, Bovan D, Jelača S, Jović Z, Purić M, Koruga D, Mijatović S, Maksimović-Ivanić D. Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo. in Nanomaterials. 2023;13(3):372.
doi:10.3390/nano13030372 .

Markelić, Milica, Mojić, Marija, Bovan, Dijana, Jelača, Sanja, Jović, Zorana, Purić, Milica, Koruga, Djuro, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo" in Nanomaterials, 13, no. 3 (2023):372,
https://doi.org/10.3390/nano13030372 . .

TY  - JOUR
AU  - Useini, Liridona
AU  - Mojić, Marija
AU  - Laube, Markus
AU  - Lönnecke, Peter
AU  - Dahme, Jonas
AU  - Sárosi, Menyhárt B.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Pietzsch, Jens
AU  - Hey-Hawkins, Evamarie
PY  - 2022
UR  - https://pubs.acs.org/doi/10.1021/acsomega.2c01523
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9301635
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5075
AB  - Mefenamic acid represents a widely used nonsteroidal anti-inflammatory drug (NSAID) to treat the pain of postoperative surgery and heavy menstrual bleeding. Like other NSAIDs, mefenamic acid inhibits the synthesis of prostaglandins by nonselectively blocking cyclooxygenase (COX) isoforms COX-1 and COX-2. For the improved selectivity of the drug and, therefore, reduced related side effects, the carborane analogues of mefenamic acid were evaluated. The ortho-, meta-, and para-carborane derivatives were synthesized in three steps: halogenation of the respective cluster, followed by a Pd-catalyzed B-N coupling and hydrolysis of the nitrile derivatives under acidic conditions. The COX inhibitory activity and cytotoxicity for different cancer cell lines revealed that the carborane analogues have stronger antitumor potential compared to their parent organic compound.
PB  - Washington: American Chemical Society
T2  - ACS Omega
T1  - Carboranyl Analogues of Mefenamic Acid and Their Biological Evaluation.
IS  - 28
VL  - 7
DO  - 10.1021/acsomega.2c01523
SP  - 24282
EP  - 24291
ER  - 

@article{
author = "Useini, Liridona and Mojić, Marija and Laube, Markus and Lönnecke, Peter and Dahme, Jonas and Sárosi, Menyhárt B. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Pietzsch, Jens and Hey-Hawkins, Evamarie",
year = "2022",
abstract = "Mefenamic acid represents a widely used nonsteroidal anti-inflammatory drug (NSAID) to treat the pain of postoperative surgery and heavy menstrual bleeding. Like other NSAIDs, mefenamic acid inhibits the synthesis of prostaglandins by nonselectively blocking cyclooxygenase (COX) isoforms COX-1 and COX-2. For the improved selectivity of the drug and, therefore, reduced related side effects, the carborane analogues of mefenamic acid were evaluated. The ortho-, meta-, and para-carborane derivatives were synthesized in three steps: halogenation of the respective cluster, followed by a Pd-catalyzed B-N coupling and hydrolysis of the nitrile derivatives under acidic conditions. The COX inhibitory activity and cytotoxicity for different cancer cell lines revealed that the carborane analogues have stronger antitumor potential compared to their parent organic compound.",
publisher = "Washington: American Chemical Society",
journal = "ACS Omega",
title = "Carboranyl Analogues of Mefenamic Acid and Their Biological Evaluation.",
number = "28",
volume = "7",
doi = "10.1021/acsomega.2c01523",
pages = "24282-24291"
}

Useini, L., Mojić, M., Laube, M., Lönnecke, P., Dahme, J., Sárosi, M. B., Mijatović, S., Maksimović-Ivanić, D., Pietzsch, J.,& Hey-Hawkins, E.. (2022). Carboranyl Analogues of Mefenamic Acid and Their Biological Evaluation.. in ACS Omega
Washington: American Chemical Society., 7(28), 24282-24291.
https://doi.org/10.1021/acsomega.2c01523

Useini L, Mojić M, Laube M, Lönnecke P, Dahme J, Sárosi MB, Mijatović S, Maksimović-Ivanić D, Pietzsch J, Hey-Hawkins E. Carboranyl Analogues of Mefenamic Acid and Their Biological Evaluation.. in ACS Omega. 2022;7(28):24282-24291.
doi:10.1021/acsomega.2c01523 .

Useini, Liridona, Mojić, Marija, Laube, Markus, Lönnecke, Peter, Dahme, Jonas, Sárosi, Menyhárt B., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Pietzsch, Jens, Hey-Hawkins, Evamarie, "Carboranyl Analogues of Mefenamic Acid and Their Biological Evaluation." in ACS Omega, 7, no. 28 (2022):24282-24291,
https://doi.org/10.1021/acsomega.2c01523 . .

TY  - JOUR
AU  - Bensing, Christian
AU  - Mojić, Marija
AU  - Bulatović, Mirna
AU  - Edeler, David
AU  - Pérez-Quintanilla, Damian
AU  - Gómez-Ruiz, Santiago
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2772950822003314
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5106
AB  - A series of nanostructured SBA-15-based materials functionalized with the tetraorganotin(IV) metallodrugs Ph3Sn(CH2)nOH (n = 3, 4, 6, 8 and 11) are synthesized and structurally characterized by different techniques used in solid-state chemistry. The cytotoxicity of both the organotin(IV) compounds and the tin-functionalized SBA-15 materials are studied against different cancer cell lines observing that the materials have similar cytotoxic activity in comparison with the free organotin compounds in terms of mass. However, considering that the percentage of active metal compound loaded into material is low, the utilization of mesoporous silica as drug vehicle clearly improves the cytotoxic effectiveness of metal-based drugs against cancer cells. One of the most potent between all tested systems is material SBA-15~Cl|Ph3Sn(CH2)8OH. Its cytotoxicity seems to come from additional mechanisms apart from apoptosis provoking cell reprogram in B16 melanoma into more mature and less aggressive phenotype. Moderated production of ROS/RNS is probably in the background of observed phenomenon. Obtained results are further confirmed in syngeneic mouse model of melanoma in C57BL6 mice. The in vivo results show that SBA-15 do not disturb tumor growth, while both Ph3Sn(CH2)8OH and SBA-15~Cl|Ph3Sn(CH2)8OH significantly decreases tumor volume with an enhancement of the antitumor potential of the tetraorganotin(IV) compound upon immobilization in SBA-15.
PB  - Elsevier B.V.
T2  - Biomaterials Advances
T1  - Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.
VL  - 140
DO  - 10.1016/j.bioadv.2022.213054
SP  - 213054
ER  - 

@article{
author = "Bensing, Christian and Mojić, Marija and Bulatović, Mirna and Edeler, David and Pérez-Quintanilla, Damian and Gómez-Ruiz, Santiago and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2022",
abstract = "A series of nanostructured SBA-15-based materials functionalized with the tetraorganotin(IV) metallodrugs Ph3Sn(CH2)nOH (n = 3, 4, 6, 8 and 11) are synthesized and structurally characterized by different techniques used in solid-state chemistry. The cytotoxicity of both the organotin(IV) compounds and the tin-functionalized SBA-15 materials are studied against different cancer cell lines observing that the materials have similar cytotoxic activity in comparison with the free organotin compounds in terms of mass. However, considering that the percentage of active metal compound loaded into material is low, the utilization of mesoporous silica as drug vehicle clearly improves the cytotoxic effectiveness of metal-based drugs against cancer cells. One of the most potent between all tested systems is material SBA-15~Cl|Ph3Sn(CH2)8OH. Its cytotoxicity seems to come from additional mechanisms apart from apoptosis provoking cell reprogram in B16 melanoma into more mature and less aggressive phenotype. Moderated production of ROS/RNS is probably in the background of observed phenomenon. Obtained results are further confirmed in syngeneic mouse model of melanoma in C57BL6 mice. The in vivo results show that SBA-15 do not disturb tumor growth, while both Ph3Sn(CH2)8OH and SBA-15~Cl|Ph3Sn(CH2)8OH significantly decreases tumor volume with an enhancement of the antitumor potential of the tetraorganotin(IV) compound upon immobilization in SBA-15.",
publisher = "Elsevier B.V.",
journal = "Biomaterials Advances",
title = "Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.",
volume = "140",
doi = "10.1016/j.bioadv.2022.213054",
pages = "213054"
}

Bensing, C., Mojić, M., Bulatović, M., Edeler, D., Pérez-Quintanilla, D., Gómez-Ruiz, S., Maksimović-Ivanić, D., Mijatović, S.,& Kaluđerović, G. N.. (2022). Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.. in Biomaterials Advances
Elsevier B.V.., 140, 213054.
https://doi.org/10.1016/j.bioadv.2022.213054

Bensing C, Mojić M, Bulatović M, Edeler D, Pérez-Quintanilla D, Gómez-Ruiz S, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.. in Biomaterials Advances. 2022;140:213054.
doi:10.1016/j.bioadv.2022.213054 .

Bensing, Christian, Mojić, Marija, Bulatović, Mirna, Edeler, David, Pérez-Quintanilla, Damian, Gómez-Ruiz, Santiago, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Kaluđerović, Goran N., "Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH." in Biomaterials Advances, 140 (2022):213054,
https://doi.org/10.1016/j.bioadv.2022.213054 . .

TY  - CONF
AU  - Markelić, Milica
AU  - Mojić, Marija
AU  - Drača, Dijana
AU  - Jelača, Sanja
AU  - Koruga, Djuro
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5285
AB  - Antioxidant and anticancer properties of fullerene C60 and especially of its polyhydroxylated, water soluble derivatives (fullerols) make them appealing for biomedical applications. In order to analyse antitumor effects of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC)1, second generation of fullerol, melanoma cells of different intracellular features and invasive potential (B16, B16-F10, A375) were treated with 3HFWC in various concentrations (0.19-100 μg/ml) for 24, 48 and 72h. Subsequently, syngeneic murine melanoma model was used (oral 3HFWC intake, 0.15 g/l). The most prominent effect of 3HFWC, both in vitro and in vivo2, was induction of cell senescence, followed by decreased proliferative capacity and tumor growth inhibition. Senescent cells remained viable in vitro, but lost ability to divide and decreased metabolic activity, due to mitochondria alterations. Our findings demonstrate pro-senescence approach in antitumor therapy which is suggested to be less aggressive than the conventional strategies based on cancer cell killing, frequently followed by compensatory proliferation and subsequent tumor progression.
PB  - Belgrade: Faculty of Chemistry
C3  - Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
T1  - Pro-senescent effects of hyper-harmonized hydroxylated fullerene water complex in melanoma
SP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5285
ER  - 

@conference{
author = "Markelić, Milica and Mojić, Marija and Drača, Dijana and Jelača, Sanja and Koruga, Djuro and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "Antioxidant and anticancer properties of fullerene C60 and especially of its polyhydroxylated, water soluble derivatives (fullerols) make them appealing for biomedical applications. In order to analyse antitumor effects of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC)1, second generation of fullerol, melanoma cells of different intracellular features and invasive potential (B16, B16-F10, A375) were treated with 3HFWC in various concentrations (0.19-100 μg/ml) for 24, 48 and 72h. Subsequently, syngeneic murine melanoma model was used (oral 3HFWC intake, 0.15 g/l). The most prominent effect of 3HFWC, both in vitro and in vivo2, was induction of cell senescence, followed by decreased proliferative capacity and tumor growth inhibition. Senescent cells remained viable in vitro, but lost ability to divide and decreased metabolic activity, due to mitochondria alterations. Our findings demonstrate pro-senescence approach in antitumor therapy which is suggested to be less aggressive than the conventional strategies based on cancer cell killing, frequently followed by compensatory proliferation and subsequent tumor progression.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia",
title = "Pro-senescent effects of hyper-harmonized hydroxylated fullerene water complex in melanoma",
pages = "95",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5285"
}

Markelić, M., Mojić, M., Drača, D., Jelača, S., Koruga, D., Mijatović, S.,& Maksimović-Ivanić, D.. (2022). Pro-senescent effects of hyper-harmonized hydroxylated fullerene water complex in melanoma. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
Belgrade: Faculty of Chemistry., 95.
https://hdl.handle.net/21.15107/rcub_ibiss_5285

Markelić M, Mojić M, Drača D, Jelača S, Koruga D, Mijatović S, Maksimović-Ivanić D. Pro-senescent effects of hyper-harmonized hydroxylated fullerene water complex in melanoma. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia. 2022;:95.
https://hdl.handle.net/21.15107/rcub_ibiss_5285 .

Markelić, Milica, Mojić, Marija, Drača, Dijana, Jelača, Sanja, Koruga, Djuro, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Pro-senescent effects of hyper-harmonized hydroxylated fullerene water complex in melanoma" in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia (2022):95,
https://hdl.handle.net/21.15107/rcub_ibiss_5285 .

TY  - JOUR
AU  - Vodnik, Vesna V.
AU  - Mojić, Marija
AU  - Stamenović, Una
AU  - Otoničar, Mojca
AU  - Ajdžanović, Vladimir
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Marković, Mirjana M.
AU  - Barudžija, Tanja
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4238
AB  - Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines
VL  - 124
DO  - 10.1016/j.msec.2021.112078
SP  - 112078
ER  - 

@article{
author = "Vodnik, Vesna V. and Mojić, Marija and Stamenović, Una and Otoničar, Mojca and Ajdžanović, Vladimir and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Marković, Mirjana M. and Barudžija, Tanja and Filipović, Branko and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2021",
abstract = "Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines",
volume = "124",
doi = "10.1016/j.msec.2021.112078",
pages = "112078"
}

Vodnik, V. V., Mojić, M., Stamenović, U., Otoničar, M., Ajdžanović, V., Maksimović-Ivanić, D., Mijatović, S., Marković, M. M., Barudžija, T., Filipović, B., Milošević, V.,& Šošić-Jurjević, B.. (2021). Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C
Elsevier Ltd., 124, 112078.
https://doi.org/10.1016/j.msec.2021.112078

Vodnik VV, Mojić M, Stamenović U, Otoničar M, Ajdžanović V, Maksimović-Ivanić D, Mijatović S, Marković MM, Barudžija T, Filipović B, Milošević V, Šošić-Jurjević B. Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C. 2021;124:112078.
doi:10.1016/j.msec.2021.112078 .

Vodnik, Vesna V., Mojić, Marija, Stamenović, Una, Otoničar, Mojca, Ajdžanović, Vladimir, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Marković, Mirjana M., Barudžija, Tanja, Filipović, Branko, Milošević, Verica, Šošić-Jurjević, Branka , "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines" in Materials Science and Engineering C, 124 (2021):112078,
https://doi.org/10.1016/j.msec.2021.112078 . .

TY  - CONF
AU  - Drača, Dijana
AU  - Markelić, Milica
AU  - Mojić, Marija
AU  - Jelača, Sanja
AU  - Mijatović, Sanja
AU  - Jović, Zorana
AU  - Dragičević, Aleksandra
AU  - Koruga, Đuro
AU  - Maksimović-Ivanić, Danijela
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4433
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
AB  - Although paucigranulocytic asthma (PGA) is the most common phenotype of stable asthma, its features are not adequately studied. In this study, we aim to display the characteristics 
of PGA. 116 non‐smoker adult asthma patients (80% female, mean age 39± 12.9) admitted to three tertiary centres were included. Their demographic and clinical features, allergy 
status, biochemical results, Asthma Control Test (ACT) scores, spirometry and exhaled nitric oxide (FeNO) measurements were obtained. Induced sputum cytometry was performed. 
According to induced sputum cell counts, four phenotypes were detected: eosinophilic (EA) (22.4%), mixed (MGA) (6.9%), neutrophilic (NA) (7.8%) and paucigranulocytic (62.9%). In 
sputum, macrophages were higher in the PGA group compared to other groups (PGA vs NA and PGA vs MGA: p<0.001, PGA vs EA: p=0.03). The atopy rate between phenotypes was 
the same. Although forced expiratory flow 1st second (FEV1) was similar in four groups, FEV1/FVC was higher (p=0.013) and FEV1 reversibility was lower in PGA patients than the 
corresponding values in other phenotypes (p=0.015). Low reversibility was comparable in both inhaled corticosteroid naive PGA patients and in those on ICS treatment. Although 
insignificant, FeNO and blood eosinophil counts were higher in MGA and EA groups while these were the lowest in the PGA group. Uncontrolled asthma ratio was low in PGA (16%) 
while it was 11% for NA, 25% for MG and 23% in EA. Macrophages are predominant in sputum of PGA patients. Besides lower uncontrolled asthma ratio, lower FEV1 reversibility is 
a prominent characteristic of this phenotype.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo
IS  - Suppl 1
VL  - 51
DO  - 10.1002/eji.202170200
SP  - 353
ER  - 

@conference{
author = "Drača, Dijana and Markelić, Milica and Mojić, Marija and Jelača, Sanja and Mijatović, Sanja and Jović, Zorana and Dragičević, Aleksandra and Koruga, Đuro and Maksimović-Ivanić, Danijela",
year = "2021",
abstract = "Although paucigranulocytic asthma (PGA) is the most common phenotype of stable asthma, its features are not adequately studied. In this study, we aim to display the characteristics 
of PGA. 116 non‐smoker adult asthma patients (80% female, mean age 39± 12.9) admitted to three tertiary centres were included. Their demographic and clinical features, allergy 
status, biochemical results, Asthma Control Test (ACT) scores, spirometry and exhaled nitric oxide (FeNO) measurements were obtained. Induced sputum cytometry was performed. 
According to induced sputum cell counts, four phenotypes were detected: eosinophilic (EA) (22.4%), mixed (MGA) (6.9%), neutrophilic (NA) (7.8%) and paucigranulocytic (62.9%). In 
sputum, macrophages were higher in the PGA group compared to other groups (PGA vs NA and PGA vs MGA: p<0.001, PGA vs EA: p=0.03). The atopy rate between phenotypes was 
the same. Although forced expiratory flow 1st second (FEV1) was similar in four groups, FEV1/FVC was higher (p=0.013) and FEV1 reversibility was lower in PGA patients than the 
corresponding values in other phenotypes (p=0.015). Low reversibility was comparable in both inhaled corticosteroid naive PGA patients and in those on ICS treatment. Although 
insignificant, FeNO and blood eosinophil counts were higher in MGA and EA groups while these were the lowest in the PGA group. Uncontrolled asthma ratio was low in PGA (16%) 
while it was 11% for NA, 25% for MG and 23% in EA. Macrophages are predominant in sputum of PGA patients. Besides lower uncontrolled asthma ratio, lower FEV1 reversibility is 
a prominent characteristic of this phenotype.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo",
number = "Suppl 1",
volume = "51",
doi = "10.1002/eji.202170200",
pages = "353"
}

Drača, D., Markelić, M., Mojić, M., Jelača, S., Mijatović, S., Jović, Z., Dragičević, A., Koruga, Đ.,& Maksimović-Ivanić, D.. (2021). Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 51(Suppl 1), 353.
https://doi.org/10.1002/eji.202170200

Drača D, Markelić M, Mojić M, Jelača S, Mijatović S, Jović Z, Dragičević A, Koruga Đ, Maksimović-Ivanić D. Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;51(Suppl 1):353.
doi:10.1002/eji.202170200 .

Drača, Dijana, Markelić, Milica, Mojić, Marija, Jelača, Sanja, Mijatović, Sanja, Jović, Zorana, Dragičević, Aleksandra, Koruga, Đuro, Maksimović-Ivanić, Danijela, "Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting, 51, no. Suppl 1 (2021):353,
https://doi.org/10.1002/eji.202170200 . .

TY  - JOUR
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Mojić, Marija
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0940960218301080?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3150
AB  - In a rat model of the andropause we aimed to examine the influence of daidzein, soy isoflavone, on the structure and function of parathyroid glands (PTG) and the expression levels of some of the crucial regulators of Ca2+ and Pi homeostasis in the kidney, and to compare these effects with the effects of estradiol, serving as a positive control. Middle-aged (16-month-old) male Wistar rats were divided into the following groups: sham-operated (SO), orchidectomized (Orx), orchidectomized and estradiol-treated (Orx+E; 0.625mg/kg b.w./day, s.c.) as well as orchidectomized and daidzein-treated (Orx+D; 30mg/kg b.w./day, s.c.) group. Every treated group had a corresponding control group. PTH serum concentration was decreased in Orx+E and Orx+D groups by 10% and 21% (p<0.05) respectively, in comparison with the Orx. PTG volume was decreased in Orx+E group by 16% (p<0.05), when compared to the Orx. In Orx+E group expression of NaPi 2a was lower (p<0.05), while NaPi 2a abundance in Orx+D animals was increased (p<0.05), when compared to Orx. Expression of PTH1R was increased (p<0.05) in Orx+E group, while in Orx+D animals the same parameter was decreased (p<0.05), in comparison with Orx. Klotho expression was elevated (p<0.05) in Orx+D rats, in regard to Orx. Orx+D induced reduction in Ca2+/creatinine and Pi/creatinine ratio in urine by 32% and 16% (p<0.05) respectively, in comparison with Orx. In conclusion, presented results indicate the more coherent beneficial effects of daidzein compared to estradiol, on disturbed Ca2+ and Pi homeostasis, and presumably on bone health, in the aging male rats.
T2  - Annals of Anatomy = Anatomischer Anzeiger
T1  - Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.
VL  - 221
DO  - 10.1016/j.aanat.2018.08.001
SP  - 27
EP  - 37
ER  - 

@article{
author = "Živanović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir and Mojić, Marija and Miler, Marko and Šošić-Jurjević, Branka  and Milošević, Verica and Filipović, Branko",
year = "2019",
abstract = "In a rat model of the andropause we aimed to examine the influence of daidzein, soy isoflavone, on the structure and function of parathyroid glands (PTG) and the expression levels of some of the crucial regulators of Ca2+ and Pi homeostasis in the kidney, and to compare these effects with the effects of estradiol, serving as a positive control. Middle-aged (16-month-old) male Wistar rats were divided into the following groups: sham-operated (SO), orchidectomized (Orx), orchidectomized and estradiol-treated (Orx+E; 0.625mg/kg b.w./day, s.c.) as well as orchidectomized and daidzein-treated (Orx+D; 30mg/kg b.w./day, s.c.) group. Every treated group had a corresponding control group. PTH serum concentration was decreased in Orx+E and Orx+D groups by 10% and 21% (p<0.05) respectively, in comparison with the Orx. PTG volume was decreased in Orx+E group by 16% (p<0.05), when compared to the Orx. In Orx+E group expression of NaPi 2a was lower (p<0.05), while NaPi 2a abundance in Orx+D animals was increased (p<0.05), when compared to Orx. Expression of PTH1R was increased (p<0.05) in Orx+E group, while in Orx+D animals the same parameter was decreased (p<0.05), in comparison with Orx. Klotho expression was elevated (p<0.05) in Orx+D rats, in regard to Orx. Orx+D induced reduction in Ca2+/creatinine and Pi/creatinine ratio in urine by 32% and 16% (p<0.05) respectively, in comparison with Orx. In conclusion, presented results indicate the more coherent beneficial effects of daidzein compared to estradiol, on disturbed Ca2+ and Pi homeostasis, and presumably on bone health, in the aging male rats.",
journal = "Annals of Anatomy = Anatomischer Anzeiger",
title = "Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.",
volume = "221",
doi = "10.1016/j.aanat.2018.08.001",
pages = "27-37"
}

Živanović, J., Jarić, I., Ajdžanović, V., Mojić, M., Miler, M., Šošić-Jurjević, B., Milošević, V.,& Filipović, B.. (2019). Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.. in Annals of Anatomy = Anatomischer Anzeiger, 221, 27-37.
https://doi.org/10.1016/j.aanat.2018.08.001

Živanović J, Jarić I, Ajdžanović V, Mojić M, Miler M, Šošić-Jurjević B, Milošević V, Filipović B. Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.. in Annals of Anatomy = Anatomischer Anzeiger. 2019;221:27-37.
doi:10.1016/j.aanat.2018.08.001 .

Živanović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, Mojić, Marija, Miler, Marko, Šošić-Jurjević, Branka , Milošević, Verica, Filipović, Branko, "Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause." in Annals of Anatomy = Anatomischer Anzeiger, 221 (2019):27-37,
https://doi.org/10.1016/j.aanat.2018.08.001 . .

TY  - JOUR
AU  - Ljujić, Mila
AU  - Mijatović, Sanja
AU  - Bulatović, Mirna
AU  - Mojić, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Radojković, Dragica
AU  - Topić, Aleksandra
PY  - 2017
UR  - http://link.springer.com/10.1007/s12253-016-0104-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2729
AB  - Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance.
T2  - Pathology & Oncology Research
T1  - Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines
IS  - 2
VL  - 23
DO  - 10.1007/s12253-016-0104-3
SP  - 335
EP  - 343
ER  - 

@article{
author = "Ljujić, Mila and Mijatović, Sanja and Bulatović, Mirna and Mojić, Marija and Maksimović-Ivanić, Danijela and Radojković, Dragica and Topić, Aleksandra",
year = "2017",
abstract = "Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance.",
journal = "Pathology & Oncology Research",
title = "Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines",
number = "2",
volume = "23",
doi = "10.1007/s12253-016-0104-3",
pages = "335-343"
}

Ljujić, M., Mijatović, S., Bulatović, M., Mojić, M., Maksimović-Ivanić, D., Radojković, D.,& Topić, A.. (2017). Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines. in Pathology & Oncology Research, 23(2), 335-343.
https://doi.org/10.1007/s12253-016-0104-3

Ljujić M, Mijatović S, Bulatović M, Mojić M, Maksimović-Ivanić D, Radojković D, Topić A. Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines. in Pathology & Oncology Research. 2017;23(2):335-343.
doi:10.1007/s12253-016-0104-3 .

Ljujić, Mila, Mijatović, Sanja, Bulatović, Mirna, Mojić, Marija, Maksimović-Ivanić, Danijela, Radojković, Dragica, Topić, Aleksandra, "Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines" in Pathology & Oncology Research, 23, no. 2 (2017):335-343,
https://doi.org/10.1007/s12253-016-0104-3 . .

TY  - JOUR
AU  - Ljujic, Mila
AU  - Mijatović, Sanja
AU  - Bulatović, Mirna
AU  - Mojić, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Radojkovic, Dragica
AU  - Topic, Aleksandra
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4166
AB  - Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-κB. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.
PB  - Moskva: Izdatelstvo Nauka
T2  - Molekuliarnaia biologiia
T1  - Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)
IS  - 1
VL  - 50
DO  - 10.7868/S0026898416010122
SP  - 174
EP  - 178
ER  - 

@article{
author = "Ljujic, Mila and Mijatović, Sanja and Bulatović, Mirna and Mojić, Marija and Maksimović-Ivanić, Danijela and Radojkovic, Dragica and Topic, Aleksandra",
year = "2016",
abstract = "Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-κB. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.",
publisher = "Moskva: Izdatelstvo Nauka",
journal = "Molekuliarnaia biologiia",
title = "Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)",
number = "1",
volume = "50",
doi = "10.7868/S0026898416010122",
pages = "174-178"
}

Ljujic, M., Mijatović, S., Bulatović, M., Mojić, M., Maksimović-Ivanić, D., Radojkovic, D.,& Topic, A.. (2016). Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molekuliarnaia biologiia
Moskva: Izdatelstvo Nauka., 50(1), 174-178.
https://doi.org/10.7868/S0026898416010122

Ljujic M, Mijatović S, Bulatović M, Mojić M, Maksimović-Ivanić D, Radojkovic D, Topic A. Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molekuliarnaia biologiia. 2016;50(1):174-178.
doi:10.7868/S0026898416010122 .

Ljujic, Mila, Mijatović, Sanja, Bulatović, Mirna, Mojić, Marija, Maksimović-Ivanić, Danijela, Radojkovic, Dragica, Topic, Aleksandra, "Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)" in Molekuliarnaia biologiia, 50, no. 1 (2016):174-178,
https://doi.org/10.7868/S0026898416010122 . .

TY  - JOUR
AU  - Bensing, Christian
AU  - Mojić, Marija
AU  - Gómez-Ruiz, Santiago
AU  - Carralero, Sandra
AU  - Dojčinović, Biljana
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2016
UR  - http://xlink.rsc.org/?DOI=C6DT03519A
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-85000774313&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=691E27A4B52E4CB98111082A19AFDEEC.wsnAw8kcdt7IPYLO0V48gA%3A11#
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2481
AB  - SBA-15|Sn3, a mesoporous silica-based material (derivative of SBA-15) loaded with an organotin compound Ph3Sn(CH2)3OH (Sn3), possesses improved antitumor potential against the A2780 high-grade serous ovarian carcinoma cell line in comparison to Sn3. It is demonstrated that both the compound and the nanostructured material are internalized by the A2780 cells. A similar mode of action of Sn3 and SBA-15|Sn3 against the A2780 cell line was found. Explicitly, induction of apoptosis, caspase 2, 3, 8 and 9 activation, accumulation of cells in the hypodiploid phase as well as accumulation of ROS were observed. Interestingly, Sn3 loaded in the mesoporous silica-based material needed to reach a concentration 3.5 times lower than the IC50 value of the Sn3 compound, pointing out a higher effect of the SBA-15|Sn3 than Sn3 alone. Clonogenic potential, growth in 3D culture as well as mobility of cells were disturbed in the presence of SBA-15|Sn3. Such behavior could be associated with the suppression of p-38 MAPK. Less profound effect of Sn3 compared to SBA-15|Sn3 could be attributed to a different regulation of p-38 and STAT-3, which are mainly responsible for an appropriate cellular response to diverse stimuli or metastatic properties.
T2  - Dalton Transactions
T1  - Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph 3 Sn(CH 2) 3 OH against the A2780 ovarian carcinoma cell line
IS  - 47
VL  - 45
DO  - 10.1039/C6DT03519A
SP  - 18984
EP  - 18993
ER  - 

@article{
author = "Bensing, Christian and Mojić, Marija and Gómez-Ruiz, Santiago and Carralero, Sandra and Dojčinović, Biljana and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2016",
abstract = "SBA-15|Sn3, a mesoporous silica-based material (derivative of SBA-15) loaded with an organotin compound Ph3Sn(CH2)3OH (Sn3), possesses improved antitumor potential against the A2780 high-grade serous ovarian carcinoma cell line in comparison to Sn3. It is demonstrated that both the compound and the nanostructured material are internalized by the A2780 cells. A similar mode of action of Sn3 and SBA-15|Sn3 against the A2780 cell line was found. Explicitly, induction of apoptosis, caspase 2, 3, 8 and 9 activation, accumulation of cells in the hypodiploid phase as well as accumulation of ROS were observed. Interestingly, Sn3 loaded in the mesoporous silica-based material needed to reach a concentration 3.5 times lower than the IC50 value of the Sn3 compound, pointing out a higher effect of the SBA-15|Sn3 than Sn3 alone. Clonogenic potential, growth in 3D culture as well as mobility of cells were disturbed in the presence of SBA-15|Sn3. Such behavior could be associated with the suppression of p-38 MAPK. Less profound effect of Sn3 compared to SBA-15|Sn3 could be attributed to a different regulation of p-38 and STAT-3, which are mainly responsible for an appropriate cellular response to diverse stimuli or metastatic properties.",
journal = "Dalton Transactions",
title = "Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph 3 Sn(CH 2) 3 OH against the A2780 ovarian carcinoma cell line",
number = "47",
volume = "45",
doi = "10.1039/C6DT03519A",
pages = "18984-18993"
}

Bensing, C., Mojić, M., Gómez-Ruiz, S., Carralero, S., Dojčinović, B., Maksimović-Ivanić, D., Mijatović, S.,& Kaluđerović, G. N.. (2016). Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph 3 Sn(CH 2) 3 OH against the A2780 ovarian carcinoma cell line. in Dalton Transactions, 45(47), 18984-18993.
https://doi.org/10.1039/C6DT03519A

Bensing C, Mojić M, Gómez-Ruiz S, Carralero S, Dojčinović B, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph 3 Sn(CH 2) 3 OH against the A2780 ovarian carcinoma cell line. in Dalton Transactions. 2016;45(47):18984-18993.
doi:10.1039/C6DT03519A .

Bensing, Christian, Mojić, Marija, Gómez-Ruiz, Santiago, Carralero, Sandra, Dojčinović, Biljana, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Kaluđerović, Goran N., "Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph 3 Sn(CH 2) 3 OH against the A2780 ovarian carcinoma cell line" in Dalton Transactions, 45, no. 47 (2016):18984-18993,
https://doi.org/10.1039/C6DT03519A . .

TY  - JOUR
AU  - Ludwig, Gerd
AU  - Mojić, Marija
AU  - Bulatović, Mirna
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Steinborn, Dirk
AU  - Kaluđerović, Goran N
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3827
AB  - In vitro studies with the ruthenium(II) and analogous iridium(III) complexes [Ru(η6- p-cymene)Cl2{Ph2PCH2CH2CH2S(O)xPh-κP}], [Ru(η6-p-cymene)Cl{Ph2PCH2CH2CH2S(O)xPh- κP,κS}][PF6] (1-4), [Ir(η5-C5Me5)Cl2{Ph2PCH2CH2CH2S(O)xPh-κP}] and [Ir(η5-C5Me5)Cl{Ph2 PCH2CH2CH2S(O)xPh-κP,κS}][PF6] (5-8; x = 0, 1) revealed the high selectivity toward the 8505C, A253, MCF-7, SW480 and 518A2 cancer cell lines. Thus, the cationic ruthenium complex 4 proved to be the most selective one. In case of the neutral and cationic ruthenium complexes 1-4 the caspase-dependent apoptotic cell death was proven as the main cause of the drug's tumoricidal action on 8505C cell line.
PB  - Sharjah: Bentham Science Publishers
T2  - Anti-Cancer Agents in Medicinal Chemistry
T1  - Biological Potential of Halfsandwich Ruthenium(II) and Iridium (III) Complexes
IS  - 11
VL  - 16
DO  - 10.2174/1871520615666151029100749
SP  - 1455
EP  - 1460
ER  - 

@article{
author = "Ludwig, Gerd and Mojić, Marija and Bulatović, Mirna and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Steinborn, Dirk and Kaluđerović, Goran N",
year = "2016",
abstract = "In vitro studies with the ruthenium(II) and analogous iridium(III) complexes [Ru(η6- p-cymene)Cl2{Ph2PCH2CH2CH2S(O)xPh-κP}], [Ru(η6-p-cymene)Cl{Ph2PCH2CH2CH2S(O)xPh- κP,κS}][PF6] (1-4), [Ir(η5-C5Me5)Cl2{Ph2PCH2CH2CH2S(O)xPh-κP}] and [Ir(η5-C5Me5)Cl{Ph2 PCH2CH2CH2S(O)xPh-κP,κS}][PF6] (5-8; x = 0, 1) revealed the high selectivity toward the 8505C, A253, MCF-7, SW480 and 518A2 cancer cell lines. Thus, the cationic ruthenium complex 4 proved to be the most selective one. In case of the neutral and cationic ruthenium complexes 1-4 the caspase-dependent apoptotic cell death was proven as the main cause of the drug's tumoricidal action on 8505C cell line.",
publisher = "Sharjah: Bentham Science Publishers",
journal = "Anti-Cancer Agents in Medicinal Chemistry",
title = "Biological Potential of Halfsandwich Ruthenium(II) and Iridium (III) Complexes",
number = "11",
volume = "16",
doi = "10.2174/1871520615666151029100749",
pages = "1455-1460"
}

Ludwig, G., Mojić, M., Bulatović, M., Mijatović, S., Maksimović-Ivanić, D., Steinborn, D.,& Kaluđerović, G. N.. (2016). Biological Potential of Halfsandwich Ruthenium(II) and Iridium (III) Complexes. in Anti-Cancer Agents in Medicinal Chemistry
Sharjah: Bentham Science Publishers., 16(11), 1455-1460.
https://doi.org/10.2174/1871520615666151029100749

Ludwig G, Mojić M, Bulatović M, Mijatović S, Maksimović-Ivanić D, Steinborn D, Kaluđerović GN. Biological Potential of Halfsandwich Ruthenium(II) and Iridium (III) Complexes. in Anti-Cancer Agents in Medicinal Chemistry. 2016;16(11):1455-1460.
doi:10.2174/1871520615666151029100749 .

Ludwig, Gerd, Mojić, Marija, Bulatović, Mirna, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Steinborn, Dirk, Kaluđerović, Goran N, "Biological Potential of Halfsandwich Ruthenium(II) and Iridium (III) Complexes" in Anti-Cancer Agents in Medicinal Chemistry, 16, no. 11 (2016):1455-1460,
https://doi.org/10.2174/1871520615666151029100749 . .

TY  - JOUR
AU  - Kaluđerović, Milena R.
AU  - Mojić, Marija
AU  - Gómez-Ruiz,  Santiago
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3826
AB  - In vitro antitumor activity of various organogallium(III) complexes (1-8) has been tested against CT26CL25, HCT116, SW480 colon cancer cell lines. CV and MTT assays were used to assess on the antiproliferative effect of investigated organogallium(III) complexes. From the investigated complexes, the most active was found to be tetranuclear compound 8 against CT26CL25 cells. Flow cytometric analysis of the CT26CL25 cells upon the treatment with 8 was performed in order to determine the role of apoptosis, caspase activation, autophagy and proliferation rate on the cell death caused with this compound. Results indicate cytotoxic potential of the tetranuclear complex 8 by inducing caspase independent apoptosis and blocking most of the cells before first division.
PB  - Sharjah: Bentham Science Publishers
T2  - Anticancer Agents Med Chem
T1  - Anticancer Activity of Organogallium(III) Complexes in Colon Cancer Cells
IS  - 3
VL  - 16
DO  - 10.2174/1871520615666151007160319
SP  - 359
EP  - 364
ER  - 

@article{
author = "Kaluđerović, Milena R. and Mojić, Marija and Gómez-Ruiz,  Santiago and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2016",
abstract = "In vitro antitumor activity of various organogallium(III) complexes (1-8) has been tested against CT26CL25, HCT116, SW480 colon cancer cell lines. CV and MTT assays were used to assess on the antiproliferative effect of investigated organogallium(III) complexes. From the investigated complexes, the most active was found to be tetranuclear compound 8 against CT26CL25 cells. Flow cytometric analysis of the CT26CL25 cells upon the treatment with 8 was performed in order to determine the role of apoptosis, caspase activation, autophagy and proliferation rate on the cell death caused with this compound. Results indicate cytotoxic potential of the tetranuclear complex 8 by inducing caspase independent apoptosis and blocking most of the cells before first division.",
publisher = "Sharjah: Bentham Science Publishers",
journal = "Anticancer Agents Med Chem",
title = "Anticancer Activity of Organogallium(III) Complexes in Colon Cancer Cells",
number = "3",
volume = "16",
doi = "10.2174/1871520615666151007160319",
pages = "359-364"
}

Kaluđerović, M. R., Mojić, M., Gómez-Ruiz,  ., Mijatović, S.,& Maksimović-Ivanić, D.. (2016). Anticancer Activity of Organogallium(III) Complexes in Colon Cancer Cells. in Anticancer Agents Med Chem
Sharjah: Bentham Science Publishers., 16(3), 359-364.
https://doi.org/10.2174/1871520615666151007160319

Kaluđerović MR, Mojić M, Gómez-Ruiz  , Mijatović S, Maksimović-Ivanić D. Anticancer Activity of Organogallium(III) Complexes in Colon Cancer Cells. in Anticancer Agents Med Chem. 2016;16(3):359-364.
doi:10.2174/1871520615666151007160319 .

Kaluđerović, Milena R., Mojić, Marija, Gómez-Ruiz,  Santiago, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Anticancer Activity of Organogallium(III) Complexes in Colon Cancer Cells" in Anticancer Agents Med Chem, 16, no. 3 (2016):359-364,
https://doi.org/10.2174/1871520615666151007160319 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Mojić, Marija
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6275
AB  - Soy isoflavone's (especially genistein and daidzein) health effects have been studied for decades, while their mechanisms of action remain complex, involving modulation of gene expression and enzyme activity. Currently there is a need for refreshed approach in this field considering identification of isoflavone's new molecular target. The cell membrane constituents-mediated effects of soy isoflavones are worthy of special attention from the perspective of cancer metastasis or cardiovascular diseases management. Specifically, the expanding concept of membrane steroid receptors and rapid signaling from the cell surface may include the prominent role of these steroid-like compounds.
We have shown in vitro that genistein inhibits LNCaP prostate cancer cells invasiveness by decreasing the membrane fluidity. This is complemented with genistein-caused immobilization of the androgen receptor containing membrane lipid rafts and down regulation of the androgen receptors/Akt signaling in mentioned metastatic prostate cancer cell line. On the other hand, it was observed that daidzein strongly couples with membrane estrogen receptors in adrenal medullary cells. At low doses (10 - 1000 nM) daidzein was found to stimulate catecholamine synthesis via extracellular signal-regulated kinase 1/2 or protein kinase A pathways, but at higher doses it inhibited catecholamine synthesis and secretion induced by acetylcholine.
Given that catecholamine excess can contribute to the cardiovascular pathologies and that catecholamine lack may lead to depression, daidzein application promises to have a wide range of therapeutic effects.
These data are supportive in development of the molecular pharmacotherapy pertinent to balanced soy isoflavone treatment of steroid-related malignancies as well as cardiovascular and psychiatric diseases.
PB  - Institut national de la recherche agronomique (INRA)
C3  - 7th International Conference on Polyphenols and Health ICPH2015; 2015 Oct 27-30; Tours, France
T1  - Soy isoflavones and membrane steroid receptors: a new horizon
EP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6275
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Živanović, Jasmina and Mojić, Marija and Ristić, Nataša and Filipović, Branko and Milošević, Verica",
year = "2015",
abstract = "Soy isoflavone's (especially genistein and daidzein) health effects have been studied for decades, while their mechanisms of action remain complex, involving modulation of gene expression and enzyme activity. Currently there is a need for refreshed approach in this field considering identification of isoflavone's new molecular target. The cell membrane constituents-mediated effects of soy isoflavones are worthy of special attention from the perspective of cancer metastasis or cardiovascular diseases management. Specifically, the expanding concept of membrane steroid receptors and rapid signaling from the cell surface may include the prominent role of these steroid-like compounds.
We have shown in vitro that genistein inhibits LNCaP prostate cancer cells invasiveness by decreasing the membrane fluidity. This is complemented with genistein-caused immobilization of the androgen receptor containing membrane lipid rafts and down regulation of the androgen receptors/Akt signaling in mentioned metastatic prostate cancer cell line. On the other hand, it was observed that daidzein strongly couples with membrane estrogen receptors in adrenal medullary cells. At low doses (10 - 1000 nM) daidzein was found to stimulate catecholamine synthesis via extracellular signal-regulated kinase 1/2 or protein kinase A pathways, but at higher doses it inhibited catecholamine synthesis and secretion induced by acetylcholine.
Given that catecholamine excess can contribute to the cardiovascular pathologies and that catecholamine lack may lead to depression, daidzein application promises to have a wide range of therapeutic effects.
These data are supportive in development of the molecular pharmacotherapy pertinent to balanced soy isoflavone treatment of steroid-related malignancies as well as cardiovascular and psychiatric diseases.",
publisher = "Institut national de la recherche agronomique (INRA)",
journal = "7th International Conference on Polyphenols and Health ICPH2015; 2015 Oct 27-30; Tours, France",
title = "Soy isoflavones and membrane steroid receptors: a new horizon",
pages = "97",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6275"
}

Ajdžanović, V., Jarić, I., Živanović, J., Mojić, M., Ristić, N., Filipović, B.,& Milošević, V.. (2015). Soy isoflavones and membrane steroid receptors: a new horizon. in 7th International Conference on Polyphenols and Health ICPH2015; 2015 Oct 27-30; Tours, France
Institut national de la recherche agronomique (INRA)..
https://hdl.handle.net/21.15107/rcub_ibiss_6275

Ajdžanović V, Jarić I, Živanović J, Mojić M, Ristić N, Filipović B, Milošević V. Soy isoflavones and membrane steroid receptors: a new horizon. in 7th International Conference on Polyphenols and Health ICPH2015; 2015 Oct 27-30; Tours, France. 2015;:null-97.
https://hdl.handle.net/21.15107/rcub_ibiss_6275 .

Ajdžanović, Vladimir, Jarić, Ivana, Živanović, Jasmina, Mojić, Marija, Ristić, Nataša, Filipović, Branko, Milošević, Verica, "Soy isoflavones and membrane steroid receptors: a new horizon" in 7th International Conference on Polyphenols and Health ICPH2015; 2015 Oct 27-30; Tours, France (2015),
https://hdl.handle.net/21.15107/rcub_ibiss_6275 .

TY  - JOUR
AU  - Maksimović-Ivanić, Danijela
AU  - Mojić, Marija
AU  - Bulatović, Mirna Z.
AU  - Radojkovic, Milica
AU  - Kuzmanovic, Milos
AU  - Ristic, Slobodan
AU  - Stošić-Grujičić, Stanislava
AU  - Miljković, Đorđe
AU  - Cavalli, Eugenio
AU  - Libra, Massimo
AU  - Fagone, Paolo
AU  - McCubrey, James
AU  - Nicoletti, Ferdinando
AU  - Mijatović, Sanja
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2353
AB  - Covalent attachment of NO to the first approved HIV protease inhibitor
   Saquinavir (Saq-NO) expands the therapeutic potential of the original
   drug. Apart from retained antiviral activity, the modified drug exerts
   strong antitumor effects and lower toxicity. In the present study, we
   have evaluated the sensitivity of different hematological malignancies
   to Saq-NO. Saq-NO efficiently diminished the viability of Jurkat, Raji,
   HL-60 and K562 cells. While Jurkat and Raji cells (established from
   pediatric patients) displayed abrogated proliferative potential, HL-60
   and K652 cells (originated from adults) exposed to Saq-NO treatment
   underwent caspase dependent apoptosis. In addition, similar sensitivity
   to Saq-NO was observed in mononuclear blood cells obtained from
   pediatric patients with acute lymphoblastic leukemia (ALL) and adult
   patients with acute myeloid leukemia (AML). Western blot analysis
   indicated p70S6 kinase as a possible intracellular target of Saq-NO
   action. Moreover, the addition of a NO moiety to Lopinavir resulted in
   improved antitumor potential as compared to the parental compound,
   suggesting that NO-derived HIV protease inhibitors are a potential new
   source of anticancer drugs with unique mode of action. (C) 2015 Elsevier
   Ltd. All rights reserved.
T2  - Leukemia Research
T1  - The NO-modified HIV protease inhibitor as a valuable drug for
 hematological malignancies: Role of p70S6K
IS  - 10
VL  - 39
DO  - 10.1016/j.leukres.2015.06.013
SP  - 1088
EP  - 1095
ER  - 

@article{
author = "Maksimović-Ivanić, Danijela and Mojić, Marija and Bulatović, Mirna Z. and Radojkovic, Milica and Kuzmanovic, Milos and Ristic, Slobodan and Stošić-Grujičić, Stanislava and Miljković, Đorđe and Cavalli, Eugenio and Libra, Massimo and Fagone, Paolo and McCubrey, James and Nicoletti, Ferdinando and Mijatović, Sanja",
year = "2015",
abstract = "Covalent attachment of NO to the first approved HIV protease inhibitor
   Saquinavir (Saq-NO) expands the therapeutic potential of the original
   drug. Apart from retained antiviral activity, the modified drug exerts
   strong antitumor effects and lower toxicity. In the present study, we
   have evaluated the sensitivity of different hematological malignancies
   to Saq-NO. Saq-NO efficiently diminished the viability of Jurkat, Raji,
   HL-60 and K562 cells. While Jurkat and Raji cells (established from
   pediatric patients) displayed abrogated proliferative potential, HL-60
   and K652 cells (originated from adults) exposed to Saq-NO treatment
   underwent caspase dependent apoptosis. In addition, similar sensitivity
   to Saq-NO was observed in mononuclear blood cells obtained from
   pediatric patients with acute lymphoblastic leukemia (ALL) and adult
   patients with acute myeloid leukemia (AML). Western blot analysis
   indicated p70S6 kinase as a possible intracellular target of Saq-NO
   action. Moreover, the addition of a NO moiety to Lopinavir resulted in
   improved antitumor potential as compared to the parental compound,
   suggesting that NO-derived HIV protease inhibitors are a potential new
   source of anticancer drugs with unique mode of action. (C) 2015 Elsevier
   Ltd. All rights reserved.",
journal = "Leukemia Research",
title = "The NO-modified HIV protease inhibitor as a valuable drug for
 hematological malignancies: Role of p70S6K",
number = "10",
volume = "39",
doi = "10.1016/j.leukres.2015.06.013",
pages = "1088-1095"
}

Maksimović-Ivanić, D., Mojić, M., Bulatović, M. Z., Radojkovic, M., Kuzmanovic, M., Ristic, S., Stošić-Grujičić, S., Miljković, Đ., Cavalli, E., Libra, M., Fagone, P., McCubrey, J., Nicoletti, F.,& Mijatović, S.. (2015). The NO-modified HIV protease inhibitor as a valuable drug for
 hematological malignancies: Role of p70S6K. in Leukemia Research, 39(10), 1088-1095.
https://doi.org/10.1016/j.leukres.2015.06.013

Maksimović-Ivanić D, Mojić M, Bulatović MZ, Radojkovic M, Kuzmanovic M, Ristic S, Stošić-Grujičić S, Miljković Đ, Cavalli E, Libra M, Fagone P, McCubrey J, Nicoletti F, Mijatović S. The NO-modified HIV protease inhibitor as a valuable drug for
 hematological malignancies: Role of p70S6K. in Leukemia Research. 2015;39(10):1088-1095.
doi:10.1016/j.leukres.2015.06.013 .

Maksimović-Ivanić, Danijela, Mojić, Marija, Bulatović, Mirna Z., Radojkovic, Milica, Kuzmanovic, Milos, Ristic, Slobodan, Stošić-Grujičić, Stanislava, Miljković, Đorđe, Cavalli, Eugenio, Libra, Massimo, Fagone, Paolo, McCubrey, James, Nicoletti, Ferdinando, Mijatović, Sanja, "The NO-modified HIV protease inhibitor as a valuable drug for
 hematological malignancies: Role of p70S6K" in Leukemia Research, 39, no. 10 (2015):1088-1095,
https://doi.org/10.1016/j.leukres.2015.06.013 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Mojić, Marija
AU  - Milošević, Verica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2010
AB  - Soy isoflavone's (genistein and daidzein in particular) biological
   significance has been thoroughly studied for decades, so we started from
   the premise that refreshed investigation approach in this field should
   consider identification of their new molecular targets. In addition to
   recently described epigenetic aspects of polyphenole action, the cell
   membrane constituents-mediated effects of soy isoflavones are worthy of
   special attention. Accordingly, the expanding concept of membrane
   steroid receptors and rapid signaling from the cell surface may include
   the prominent role of these steroid-like compounds. It was observed that
   daidzein strongly interacts with membrane estrogen receptors in adrenal
   medullary cells. At low doses, daidzein was found to stimulate
   catecholamine synthesis through extracellular signal-regulated kinase
   1/2 or protein kinase A pathways, but at high doses, it inhibited
   catecholamine synthesis and secretion induced by acetylcholine. Keeping
   in mind that catecholamine excess can contribute to the cardiovascular
   pathologies and that catecholamine lack may lead to depression, daidzein
   application promises to have a wide range of therapeutic effects. On the
   other hand, it was shown in vitro that genistein inhibits LNCaP prostate
   cancer cells invasiveness by decreasing the membrane fluidity along with
   immobilization of the androgen receptor containing membrane lipid rafts,
   with down regulation of the androgen receptors and Akt signaling. These
   data are promising in development of the molecular pharmacotherapy
   pertinent to balanced soy isoflavone treatment of cardiovascular,
   psychiatric, and steroid-related malignant diseases.
T2  - Journal of Membrane Biology
T1  - Membrane Steroid Receptor-Mediated Action of Soy Isoflavones: Tip of the
 Iceberg
IS  - 1
VL  - 248
DO  - 10.1007/s00232-014-9745-x
SP  - 1
EP  - 6
ER  - 

@article{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Živanović, Jasmina and Mojić, Marija and Milošević, Verica",
year = "2015",
abstract = "Soy isoflavone's (genistein and daidzein in particular) biological
   significance has been thoroughly studied for decades, so we started from
   the premise that refreshed investigation approach in this field should
   consider identification of their new molecular targets. In addition to
   recently described epigenetic aspects of polyphenole action, the cell
   membrane constituents-mediated effects of soy isoflavones are worthy of
   special attention. Accordingly, the expanding concept of membrane
   steroid receptors and rapid signaling from the cell surface may include
   the prominent role of these steroid-like compounds. It was observed that
   daidzein strongly interacts with membrane estrogen receptors in adrenal
   medullary cells. At low doses, daidzein was found to stimulate
   catecholamine synthesis through extracellular signal-regulated kinase
   1/2 or protein kinase A pathways, but at high doses, it inhibited
   catecholamine synthesis and secretion induced by acetylcholine. Keeping
   in mind that catecholamine excess can contribute to the cardiovascular
   pathologies and that catecholamine lack may lead to depression, daidzein
   application promises to have a wide range of therapeutic effects. On the
   other hand, it was shown in vitro that genistein inhibits LNCaP prostate
   cancer cells invasiveness by decreasing the membrane fluidity along with
   immobilization of the androgen receptor containing membrane lipid rafts,
   with down regulation of the androgen receptors and Akt signaling. These
   data are promising in development of the molecular pharmacotherapy
   pertinent to balanced soy isoflavone treatment of cardiovascular,
   psychiatric, and steroid-related malignant diseases.",
journal = "Journal of Membrane Biology",
title = "Membrane Steroid Receptor-Mediated Action of Soy Isoflavones: Tip of the
 Iceberg",
number = "1",
volume = "248",
doi = "10.1007/s00232-014-9745-x",
pages = "1-6"
}

Ajdžanović, V., Jarić, I., Živanović, J., Mojić, M.,& Milošević, V.. (2015). Membrane Steroid Receptor-Mediated Action of Soy Isoflavones: Tip of the
 Iceberg. in Journal of Membrane Biology, 248(1), 1-6.
https://doi.org/10.1007/s00232-014-9745-x

Ajdžanović V, Jarić I, Živanović J, Mojić M, Milošević V. Membrane Steroid Receptor-Mediated Action of Soy Isoflavones: Tip of the
 Iceberg. in Journal of Membrane Biology. 2015;248(1):1-6.
doi:10.1007/s00232-014-9745-x .

Ajdžanović, Vladimir, Jarić, Ivana, Živanović, Jasmina, Mojić, Marija, Milošević, Verica, "Membrane Steroid Receptor-Mediated Action of Soy Isoflavones: Tip of the
 Iceberg" in Journal of Membrane Biology, 248, no. 1 (2015):1-6,
https://doi.org/10.1007/s00232-014-9745-x . .

TY  - JOUR
AU  - Eichhorn, Thomas
AU  - Hey-Hawkins, Evamarie
AU  - Maksimović-Ivanić, Danijela
AU  - Mojić, Marija
AU  - Schmidt, Juergen
AU  - Mijatović, Sanja
AU  - Schmidt, Harry
AU  - Kaluđerović, Goran N.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2099
AB  - Neutral binuclear ruthenium complexes 1, 2, 3, 4, 5, 6, 7, 8 of the
   general formula {[}\{RuCl2((6)-p-cym)\}(2)-(NN)] (NN=bis(nicotinate)-
   and bis(isonicotinate)-polyethylene glycol esters:
   (3-py)COO(CH2CH2O)(n)CO(3-py) and (4-py)COO(CH2CH2O)(n)CO(4-py), n
   =1-4), as well as mononuclear
   {[}RuCl2((6)-p-cym)((3-py)COO(CH2CH2OCH3)-N)], complex 9, were
   synthesized and characterized using elemental analysis and electrospray
   ionization high-resolution mass spectrometry, infrared, H-1 NMR and C-13
   NMR spectroscopies. Stability of the binuclear complexes in the presence
   of dimethylsulfoxide was studied. Furthermore, formation of a cationic
   complex containing bridging pyridine-based bidentate ligand was
   monitored using H-1 NMR spectroscopy. Ligand precursors, polyethylene
   glycol esters of nicotinic (L12HCl-L42HCl and L9HCl) and isonicotinic
   acid dihydrochlorides (L52HCl-L82HCl), binuclear ruthenium(II) complexes
   1, 2, 3, 4, 5, 6, 7, 8 and mononuclear complex 9 were tested for in
   vitro cytotoxicity against 518A2 (melanoma), 8505C (anaplastic thyroid
   cancer), A253 (head and neck tumour), MCF-7 (breast tumour) and SW480
   (colon carcinoma) cell lines. Copyright (c) 2014 John Wiley \& Sons,
   Ltd.
T2  - Applied Organometallic Chemistry
T1  - Binuclear dichlorido(eta(6)-p-cymene)ruthenium(II) complexes with
 bis(nicotinate)- and bis (isonicotinate)-polyethylene glycol ester
 ligands
IS  - 1
VL  - 29
DO  - 10.1002/aoc.3238
SP  - 20
EP  - 25
ER  - 

@article{
author = "Eichhorn, Thomas and Hey-Hawkins, Evamarie and Maksimović-Ivanić, Danijela and Mojić, Marija and Schmidt, Juergen and Mijatović, Sanja and Schmidt, Harry and Kaluđerović, Goran N.",
year = "2015",
abstract = "Neutral binuclear ruthenium complexes 1, 2, 3, 4, 5, 6, 7, 8 of the
   general formula {[}\{RuCl2((6)-p-cym)\}(2)-(NN)] (NN=bis(nicotinate)-
   and bis(isonicotinate)-polyethylene glycol esters:
   (3-py)COO(CH2CH2O)(n)CO(3-py) and (4-py)COO(CH2CH2O)(n)CO(4-py), n
   =1-4), as well as mononuclear
   {[}RuCl2((6)-p-cym)((3-py)COO(CH2CH2OCH3)-N)], complex 9, were
   synthesized and characterized using elemental analysis and electrospray
   ionization high-resolution mass spectrometry, infrared, H-1 NMR and C-13
   NMR spectroscopies. Stability of the binuclear complexes in the presence
   of dimethylsulfoxide was studied. Furthermore, formation of a cationic
   complex containing bridging pyridine-based bidentate ligand was
   monitored using H-1 NMR spectroscopy. Ligand precursors, polyethylene
   glycol esters of nicotinic (L12HCl-L42HCl and L9HCl) and isonicotinic
   acid dihydrochlorides (L52HCl-L82HCl), binuclear ruthenium(II) complexes
   1, 2, 3, 4, 5, 6, 7, 8 and mononuclear complex 9 were tested for in
   vitro cytotoxicity against 518A2 (melanoma), 8505C (anaplastic thyroid
   cancer), A253 (head and neck tumour), MCF-7 (breast tumour) and SW480
   (colon carcinoma) cell lines. Copyright (c) 2014 John Wiley \& Sons,
   Ltd.",
journal = "Applied Organometallic Chemistry",
title = "Binuclear dichlorido(eta(6)-p-cymene)ruthenium(II) complexes with
 bis(nicotinate)- and bis (isonicotinate)-polyethylene glycol ester
 ligands",
number = "1",
volume = "29",
doi = "10.1002/aoc.3238",
pages = "20-25"
}

Eichhorn, T., Hey-Hawkins, E., Maksimović-Ivanić, D., Mojić, M., Schmidt, J., Mijatović, S., Schmidt, H.,& Kaluđerović, G. N.. (2015). Binuclear dichlorido(eta(6)-p-cymene)ruthenium(II) complexes with
 bis(nicotinate)- and bis (isonicotinate)-polyethylene glycol ester
 ligands. in Applied Organometallic Chemistry, 29(1), 20-25.
https://doi.org/10.1002/aoc.3238

Eichhorn T, Hey-Hawkins E, Maksimović-Ivanić D, Mojić M, Schmidt J, Mijatović S, Schmidt H, Kaluđerović GN. Binuclear dichlorido(eta(6)-p-cymene)ruthenium(II) complexes with
 bis(nicotinate)- and bis (isonicotinate)-polyethylene glycol ester
 ligands. in Applied Organometallic Chemistry. 2015;29(1):20-25.
doi:10.1002/aoc.3238 .

Eichhorn, Thomas, Hey-Hawkins, Evamarie, Maksimović-Ivanić, Danijela, Mojić, Marija, Schmidt, Juergen, Mijatović, Sanja, Schmidt, Harry, Kaluđerović, Goran N., "Binuclear dichlorido(eta(6)-p-cymene)ruthenium(II) complexes with
 bis(nicotinate)- and bis (isonicotinate)-polyethylene glycol ester
 ligands" in Applied Organometallic Chemistry, 29, no. 1 (2015):20-25,
https://doi.org/10.1002/aoc.3238 . .

TY  - JOUR
AU  - Bulatović, Mirna Z.
AU  - Kaluderovic, Milena R.
AU  - Mojić, Marija
AU  - Zmejkovski, Bojana B.
AU  - Hey-Hawkins, Evamarie
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Kaluđerović, Goran N.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1916
AB  - (O,O'-Diisobutyl-ethylenediamine-N,N'-di-3-propionate)tetrachloridoplati
   num(IV), {[}PtCl4(iBu(2)eddp)]. shows an improved pharmacological
   profile in comparison to cisplatin. This is manifested through
   accelerated dying process led by necrotic cell death, reflected through
   mitochondrial collapse, strong ATP depletion and reactive oxygen species
   production. Loss of mitochondrial potential was further followed with
   intensive apoptosis that finalized with DNA fragmentation.
   Different dynamic of tumoricidal action could be partly ascribed to less
   affected repair mechanisms in comparison to cisplatin. Importantly,
   {[}PtCl4(iBu(2)eddp)] did not induce necrosis in primary fibroblasts
   suggesting different intracellular response of normal vs. tumor cells.
   This selectivity toward malignant phenotype is further confirmed by
   retained tumoricidal potential in hypoxic conditions, while cisplatin
   became completely inefficient. (C) 2015 Published by Elsevier B.V.
T2  - European Journal of Pharmacology
T1  - Improved in vitro antitumor potential of
 (O,O'-Diisobutyl-ethylenediamine-N,N'-di-3-propionate)tetrachloridoplati
 num(IV) complex under normoxic and hypoxic conditions
VL  - 760
DO  - 10.1016/j.ejphar.2015.04.012
SP  - 136
EP  - 144
ER  - 

@article{
author = "Bulatović, Mirna Z. and Kaluderovic, Milena R. and Mojić, Marija and Zmejkovski, Bojana B. and Hey-Hawkins, Evamarie and Vidaković, Melita and Grdović, Nevena and Kaluđerović, Goran N. and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2015",
abstract = "(O,O'-Diisobutyl-ethylenediamine-N,N'-di-3-propionate)tetrachloridoplati
   num(IV), {[}PtCl4(iBu(2)eddp)]. shows an improved pharmacological
   profile in comparison to cisplatin. This is manifested through
   accelerated dying process led by necrotic cell death, reflected through
   mitochondrial collapse, strong ATP depletion and reactive oxygen species
   production. Loss of mitochondrial potential was further followed with
   intensive apoptosis that finalized with DNA fragmentation.
   Different dynamic of tumoricidal action could be partly ascribed to less
   affected repair mechanisms in comparison to cisplatin. Importantly,
   {[}PtCl4(iBu(2)eddp)] did not induce necrosis in primary fibroblasts
   suggesting different intracellular response of normal vs. tumor cells.
   This selectivity toward malignant phenotype is further confirmed by
   retained tumoricidal potential in hypoxic conditions, while cisplatin
   became completely inefficient. (C) 2015 Published by Elsevier B.V.",
journal = "European Journal of Pharmacology",
title = "Improved in vitro antitumor potential of
 (O,O'-Diisobutyl-ethylenediamine-N,N'-di-3-propionate)tetrachloridoplati
 num(IV) complex under normoxic and hypoxic conditions",
volume = "760",
doi = "10.1016/j.ejphar.2015.04.012",
pages = "136-144"
}

Bulatović, M. Z., Kaluderovic, M. R., Mojić, M., Zmejkovski, B. B., Hey-Hawkins, E., Vidaković, M., Grdović, N., Kaluđerović, G. N., Mijatović, S.,& Maksimović-Ivanić, D.. (2015). Improved in vitro antitumor potential of
 (O,O'-Diisobutyl-ethylenediamine-N,N'-di-3-propionate)tetrachloridoplati
 num(IV) complex under normoxic and hypoxic conditions. in European Journal of Pharmacology, 760, 136-144.
https://doi.org/10.1016/j.ejphar.2015.04.012

Bulatović MZ, Kaluderovic MR, Mojić M, Zmejkovski BB, Hey-Hawkins E, Vidaković M, Grdović N, Kaluđerović GN, Mijatović S, Maksimović-Ivanić D. Improved in vitro antitumor potential of
 (O,O'-Diisobutyl-ethylenediamine-N,N'-di-3-propionate)tetrachloridoplati
 num(IV) complex under normoxic and hypoxic conditions. in European Journal of Pharmacology. 2015;760:136-144.
doi:10.1016/j.ejphar.2015.04.012 .

Bulatović, Mirna Z., Kaluderovic, Milena R., Mojić, Marija, Zmejkovski, Bojana B., Hey-Hawkins, Evamarie, Vidaković, Melita, Grdović, Nevena, Kaluđerović, Goran N., Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Improved in vitro antitumor potential of
 (O,O'-Diisobutyl-ethylenediamine-N,N'-di-3-propionate)tetrachloridoplati
 num(IV) complex under normoxic and hypoxic conditions" in European Journal of Pharmacology, 760 (2015):136-144,
https://doi.org/10.1016/j.ejphar.2015.04.012 . .

TY  - CONF
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Mojić, Marija
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6325
AB  - Antiageing Klotho protein has a multiple regulation functions, including the one in the mineral homeostasis. It is expressed in the kidneys, parathyroid glands and choroid plexus. Phytoestrogen genistein is recognised for the prevention of osteoporosis. The aim of this study was to examine the effects of genistein on the Klotho expression in the kidneys and related mineral and parathormone concentrations, in an animal model of the andropause.
Wistar male rats (16-month-old) were divided as follows: sham operated (SO), orchidectomized (Orx) and genistein treated orchidectomised (G) group. Genistein (30 mg/kg b.w.) was subcutaneously applicated, while the control groups received the vehicle alone. Kidney Klotho expression was assessed by RT-PCR and Western blot, while the serum and urine parameters were determined biochemically.
Serum Ca2+ and Pi concentrations were decreased after Orx, while urine Ca2+ and Pi concentrations were increased after Orx, when compared to SO group.  Orx caused increase of parathormone concentration in comparison with SO animals, while G decreased the same parameter compared to Orx rats. G increased the serum Ca2+ and Pi concentrations, while Ca2+ and Pi urine concentrations were decreased comparing to Orx animals. Finally, G induced the increase of the Klotho mRNA together with the increase of protein expression when compared to Orx group.
Our results demonstrated that genistein treatment increases Klotho expression in the kidney of andropausal rats. Presented data encourage some further investigations with a view to explore the exact mechanism of genistein action in the kidneys, important for treatment of disturbed mineral homeostasis in andropause.
PB  - European Society of Endocrinology
C3  - European Young Endocrine Scientists Meeting 2014; 2014 Sep 24-26; Belgrade, Serbia
T1  - Genistein changes Klotho protein expression and PTH level in an animal model of the andropause
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6325
ER  - 

@conference{
author = "Živanović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir and Miler, Marko and Mojić, Marija and Filipović, Branko and Milošević, Verica",
year = "2014",
abstract = "Antiageing Klotho protein has a multiple regulation functions, including the one in the mineral homeostasis. It is expressed in the kidneys, parathyroid glands and choroid plexus. Phytoestrogen genistein is recognised for the prevention of osteoporosis. The aim of this study was to examine the effects of genistein on the Klotho expression in the kidneys and related mineral and parathormone concentrations, in an animal model of the andropause.
Wistar male rats (16-month-old) were divided as follows: sham operated (SO), orchidectomized (Orx) and genistein treated orchidectomised (G) group. Genistein (30 mg/kg b.w.) was subcutaneously applicated, while the control groups received the vehicle alone. Kidney Klotho expression was assessed by RT-PCR and Western blot, while the serum and urine parameters were determined biochemically.
Serum Ca2+ and Pi concentrations were decreased after Orx, while urine Ca2+ and Pi concentrations were increased after Orx, when compared to SO group.  Orx caused increase of parathormone concentration in comparison with SO animals, while G decreased the same parameter compared to Orx rats. G increased the serum Ca2+ and Pi concentrations, while Ca2+ and Pi urine concentrations were decreased comparing to Orx animals. Finally, G induced the increase of the Klotho mRNA together with the increase of protein expression when compared to Orx group.
Our results demonstrated that genistein treatment increases Klotho expression in the kidney of andropausal rats. Presented data encourage some further investigations with a view to explore the exact mechanism of genistein action in the kidneys, important for treatment of disturbed mineral homeostasis in andropause.",
publisher = "European Society of Endocrinology",
journal = "European Young Endocrine Scientists Meeting 2014; 2014 Sep 24-26; Belgrade, Serbia",
title = "Genistein changes Klotho protein expression and PTH level in an animal model of the andropause",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6325"
}

Živanović, J., Jarić, I., Ajdžanović, V., Miler, M., Mojić, M., Filipović, B.,& Milošević, V.. (2014). Genistein changes Klotho protein expression and PTH level in an animal model of the andropause. in European Young Endocrine Scientists Meeting 2014; 2014 Sep 24-26; Belgrade, Serbia
European Society of Endocrinology., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_6325

Živanović J, Jarić I, Ajdžanović V, Miler M, Mojić M, Filipović B, Milošević V. Genistein changes Klotho protein expression and PTH level in an animal model of the andropause. in European Young Endocrine Scientists Meeting 2014; 2014 Sep 24-26; Belgrade, Serbia. 2014;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_6325 .

Živanović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, Miler, Marko, Mojić, Marija, Filipović, Branko, Milošević, Verica, "Genistein changes Klotho protein expression and PTH level in an animal model of the andropause" in European Young Endocrine Scientists Meeting 2014; 2014 Sep 24-26; Belgrade, Serbia (2014):24,
https://hdl.handle.net/21.15107/rcub_ibiss_6325 .

TY  - JOUR
AU  - Mijatović, Sanja
AU  - Bulatović, Mirna Z.
AU  - Mojić, Marija
AU  - Stošić-Grujičić, Stanislava
AU  - Miljković, Đorđe
AU  - Maksimović-Ivanić, Danijela
AU  - Gomez-Ruiz, Santiago
AU  - Pinkas, Jiri
AU  - Horacek, Michal
AU  - Kaluđerović, Goran N.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2247
AB  - The previously known complexes {[}Ti\{(Me2CMe2C)(eta(5)-C5H4)(2)\}Cl-2]
   (1), {[}Ti\{Me2C(eta(5)-C5H4)(2)\}Cl-2] (2), {[}Ti
   \{Me2Si(eta(5)-C5H4)(2)\}Cl-2] (4), {[}Ti\{MePhSi(eta(5)-C5H4)(2)\}Cl-2]
   (5) and {[}Ti\{MePhSi(eta(5)-C5Me4)(2)\}Cl-2] (6) have been prepared
   following reported procedures. The novel complex
   {[}Ti\{MePhC(eta(5)-C5H4)(2)\}Cl-2] (3) has been prepared and
   characterized. The cytotoxic activity of 1-6 has been tested after 72 h
   on melanoma A375 and B16, prostate cancer DU145 and LNCaP and colon
   cancer HCT116, SW620 and CT26CL25 cell lines observing a high cytotoxic
   activity of complexes 1 and 6 compared to the reference compound
   ({[}Ti(eta(5)-C5H5)(2)\}Cl-2]). 1 and 6 have also been tested against
   primary normal mouse keratinocytes and lung fibroblasts. While viability
   of both type of primary cells was significantly less affected by 1 in
   comparison to the reference compound {[}Ti(eta(5)-C5H5)(2)Cl-2],
   compound 6 was completely nontoxic for nonmalignant cells, indicating a
   potential selectivity of this compound towards cancer cell lines. In
   addition CFSE staining, cell cycle analysis, AnnexinV-FITC/PI staining,
   detection of caspase activity and mitochondrial potential showed that 1
   and 6 were acting through inhibition of proliferation and subsequent
   induction of mitochondrial dependent apoptosis in colon cancer cell
   lines, HCT116 and SW620, which express low sensitivity to cisplatin.
   Compound 6 was found to be the leading drug in this group since it shows
   the fastest and most selective anticancer profile. (C) 2013 Elsevier
   B.V. All rights reserved.
T2  - Journal of Organometallic Chemistry
T1  - Study of the anticancer properties of methyl- and phenyl-substituted
 carbon- and silicon-bridged ansa-titanocene complexes
VL  - 751
DO  - 10.1016/j.jorganchem.2013.07.059
SP  - 361
EP  - 367
ER  - 

@article{
author = "Mijatović, Sanja and Bulatović, Mirna Z. and Mojić, Marija and Stošić-Grujičić, Stanislava and Miljković, Đorđe and Maksimović-Ivanić, Danijela and Gomez-Ruiz, Santiago and Pinkas, Jiri and Horacek, Michal and Kaluđerović, Goran N.",
year = "2014",
abstract = "The previously known complexes {[}Ti\{(Me2CMe2C)(eta(5)-C5H4)(2)\}Cl-2]
   (1), {[}Ti\{Me2C(eta(5)-C5H4)(2)\}Cl-2] (2), {[}Ti
   \{Me2Si(eta(5)-C5H4)(2)\}Cl-2] (4), {[}Ti\{MePhSi(eta(5)-C5H4)(2)\}Cl-2]
   (5) and {[}Ti\{MePhSi(eta(5)-C5Me4)(2)\}Cl-2] (6) have been prepared
   following reported procedures. The novel complex
   {[}Ti\{MePhC(eta(5)-C5H4)(2)\}Cl-2] (3) has been prepared and
   characterized. The cytotoxic activity of 1-6 has been tested after 72 h
   on melanoma A375 and B16, prostate cancer DU145 and LNCaP and colon
   cancer HCT116, SW620 and CT26CL25 cell lines observing a high cytotoxic
   activity of complexes 1 and 6 compared to the reference compound
   ({[}Ti(eta(5)-C5H5)(2)\}Cl-2]). 1 and 6 have also been tested against
   primary normal mouse keratinocytes and lung fibroblasts. While viability
   of both type of primary cells was significantly less affected by 1 in
   comparison to the reference compound {[}Ti(eta(5)-C5H5)(2)Cl-2],
   compound 6 was completely nontoxic for nonmalignant cells, indicating a
   potential selectivity of this compound towards cancer cell lines. In
   addition CFSE staining, cell cycle analysis, AnnexinV-FITC/PI staining,
   detection of caspase activity and mitochondrial potential showed that 1
   and 6 were acting through inhibition of proliferation and subsequent
   induction of mitochondrial dependent apoptosis in colon cancer cell
   lines, HCT116 and SW620, which express low sensitivity to cisplatin.
   Compound 6 was found to be the leading drug in this group since it shows
   the fastest and most selective anticancer profile. (C) 2013 Elsevier
   B.V. All rights reserved.",
journal = "Journal of Organometallic Chemistry",
title = "Study of the anticancer properties of methyl- and phenyl-substituted
 carbon- and silicon-bridged ansa-titanocene complexes",
volume = "751",
doi = "10.1016/j.jorganchem.2013.07.059",
pages = "361-367"
}

Mijatović, S., Bulatović, M. Z., Mojić, M., Stošić-Grujičić, S., Miljković, Đ., Maksimović-Ivanić, D., Gomez-Ruiz, S., Pinkas, J., Horacek, M.,& Kaluđerović, G. N.. (2014). Study of the anticancer properties of methyl- and phenyl-substituted
 carbon- and silicon-bridged ansa-titanocene complexes. in Journal of Organometallic Chemistry, 751, 361-367.
https://doi.org/10.1016/j.jorganchem.2013.07.059

Mijatović S, Bulatović MZ, Mojić M, Stošić-Grujičić S, Miljković Đ, Maksimović-Ivanić D, Gomez-Ruiz S, Pinkas J, Horacek M, Kaluđerović GN. Study of the anticancer properties of methyl- and phenyl-substituted
 carbon- and silicon-bridged ansa-titanocene complexes. in Journal of Organometallic Chemistry. 2014;751:361-367.
doi:10.1016/j.jorganchem.2013.07.059 .

Mijatović, Sanja, Bulatović, Mirna Z., Mojić, Marija, Stošić-Grujičić, Stanislava, Miljković, Đorđe, Maksimović-Ivanić, Danijela, Gomez-Ruiz, Santiago, Pinkas, Jiri, Horacek, Michal, Kaluđerović, Goran N., "Study of the anticancer properties of methyl- and phenyl-substituted
 carbon- and silicon-bridged ansa-titanocene complexes" in Journal of Organometallic Chemistry, 751 (2014):361-367,
https://doi.org/10.1016/j.jorganchem.2013.07.059 . .

TY  - JOUR
AU  - Mojić, Marija
AU  - Savic, Aleksandar
AU  - Arion, Vladimir B.
AU  - Bulatović, Mirna Z.
AU  - Poljarevic, Jelena M.
AU  - Miljković, Đorđe
AU  - Sabo, Tibor J.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Grguric-Sipka, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2310
AB  - Two p-cymene ruthenium chlorido complexes containing isobutyl (C1) and
   isoamyl (C2) esters of (S,S)ethylenediamine-N,N'-di-2-(3-cyclohexyl)
   propanoic acid as ligands were prepared from p-cymene ruthenium
   dichloride dimer and corresponding ester. All compounds have been
   characterized by elemental analysis, IR, ESI-MS, H-1 and C-13 NMR
   spectroscopy. Single crystal X-ray structure diffraction analysis of C1
   shows the usual piano-stool geometry of complexes, with coordination of
   ester ligand via nitrogen donor atoms. Ligands exhibit moderate
   anticancer activity (IC50 > 50 mu M), while the complexes were
   significantly more cytotoxic towards various cancer cell lines,
   including B16, A375, HCT116, A549 and MCF7 cells (IC50 min.-max. 2.9-8.0
   mu M). We stress that cisplatin resistant HCT116 cell line was highly
   sensitive to the treatment with C1 and C2 (IC50 values: 4.4 and 5.5 mu M
   versus IC50 > 120 mu M for cisplatin). In parallel, primary
   fibroblasts-MRC-5 were remarkably less affected by these compounds. (C)
   2013 Elsevier B. V. All rights reserved.
T2  - Journal of Organometallic Chemistry
T1  - Synthesis, X-ray structure and strong in vitro cytotoxicity of novel
 organoruthenium complexes
VL  - 749
DO  - 10.1016/j.jorganchem.2013.08.041
SP  - 142
EP  - 149
ER  - 

@article{
author = "Mojić, Marija and Savic, Aleksandar and Arion, Vladimir B. and Bulatović, Mirna Z. and Poljarevic, Jelena M. and Miljković, Đorđe and Sabo, Tibor J. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Grguric-Sipka, Sanja",
year = "2014",
abstract = "Two p-cymene ruthenium chlorido complexes containing isobutyl (C1) and
   isoamyl (C2) esters of (S,S)ethylenediamine-N,N'-di-2-(3-cyclohexyl)
   propanoic acid as ligands were prepared from p-cymene ruthenium
   dichloride dimer and corresponding ester. All compounds have been
   characterized by elemental analysis, IR, ESI-MS, H-1 and C-13 NMR
   spectroscopy. Single crystal X-ray structure diffraction analysis of C1
   shows the usual piano-stool geometry of complexes, with coordination of
   ester ligand via nitrogen donor atoms. Ligands exhibit moderate
   anticancer activity (IC50 > 50 mu M), while the complexes were
   significantly more cytotoxic towards various cancer cell lines,
   including B16, A375, HCT116, A549 and MCF7 cells (IC50 min.-max. 2.9-8.0
   mu M). We stress that cisplatin resistant HCT116 cell line was highly
   sensitive to the treatment with C1 and C2 (IC50 values: 4.4 and 5.5 mu M
   versus IC50 > 120 mu M for cisplatin). In parallel, primary
   fibroblasts-MRC-5 were remarkably less affected by these compounds. (C)
   2013 Elsevier B. V. All rights reserved.",
journal = "Journal of Organometallic Chemistry",
title = "Synthesis, X-ray structure and strong in vitro cytotoxicity of novel
 organoruthenium complexes",
volume = "749",
doi = "10.1016/j.jorganchem.2013.08.041",
pages = "142-149"
}

Mojić, M., Savic, A., Arion, V. B., Bulatović, M. Z., Poljarevic, J. M., Miljković, Đ., Sabo, T. J., Mijatović, S., Maksimović-Ivanić, D.,& Grguric-Sipka, S.. (2014). Synthesis, X-ray structure and strong in vitro cytotoxicity of novel
 organoruthenium complexes. in Journal of Organometallic Chemistry, 749, 142-149.
https://doi.org/10.1016/j.jorganchem.2013.08.041

Mojić M, Savic A, Arion VB, Bulatović MZ, Poljarevic JM, Miljković Đ, Sabo TJ, Mijatović S, Maksimović-Ivanić D, Grguric-Sipka S. Synthesis, X-ray structure and strong in vitro cytotoxicity of novel
 organoruthenium complexes. in Journal of Organometallic Chemistry. 2014;749:142-149.
doi:10.1016/j.jorganchem.2013.08.041 .

Mojić, Marija, Savic, Aleksandar, Arion, Vladimir B., Bulatović, Mirna Z., Poljarevic, Jelena M., Miljković, Đorđe, Sabo, Tibor J., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Grguric-Sipka, Sanja, "Synthesis, X-ray structure and strong in vitro cytotoxicity of novel
 organoruthenium complexes" in Journal of Organometallic Chemistry, 749 (2014):142-149,
https://doi.org/10.1016/j.jorganchem.2013.08.041 . .

TY  - JOUR
AU  - Bulatović, Mirna Z.
AU  - Maksimović-Ivanić, Danijela
AU  - Bensing, Christian
AU  - Gomez-Ruiz, Santiago
AU  - Steinborn, Dirk
AU  - Schmidt, Harry
AU  - Mojić, Marija
AU  - Korac, Aleksandra
AU  - Golic, Igor
AU  - Perez-Quintanilla, Damian
AU  - Momčilović, Miljana
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2205
AB  - The strong therapeutic potential of an organotin(IV) compound loaded in
   nanostructured silica (SBA-15pSn) is demonstrated: B16 melanoma tumor
   growth in syngeneic C57BL/6 mice is almost completely abolished. In
   contrast to apoptosis as the basic mechanism of the anticancer action of
   numerous chemotherapeutics, the important advantage of this SBA-15pSn
   mesoporous material is the induction of cell differentiation, an effect
   unknown for metal-based drugs and nanomaterials alone. This
   non-aggressive mode of drug action is highly efficient against cancer
   cells but is in the concentration range used nontoxic for normal tissue.
   JNK (Jun-amino-terminal kinase)-independent apoptosis accompanied by the
   development of the melanocyte-like nonproliferative phenotype of
   survived cells indicates the extraordinary potential of SBA-15pSn to
   suppress tumor growth without undesirable compensatory proliferation of
   malignant cells in response to neighboring cell death.
T2  - Angewandte Chemie-International Edition
T1  - Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment
IS  - 23
VL  - 53
DO  - 10.1002/anie.201400763
SP  - 5982
EP  - 5987
ER  - 

@article{
author = "Bulatović, Mirna Z. and Maksimović-Ivanić, Danijela and Bensing, Christian and Gomez-Ruiz, Santiago and Steinborn, Dirk and Schmidt, Harry and Mojić, Marija and Korac, Aleksandra and Golic, Igor and Perez-Quintanilla, Damian and Momčilović, Miljana and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2014",
abstract = "The strong therapeutic potential of an organotin(IV) compound loaded in
   nanostructured silica (SBA-15pSn) is demonstrated: B16 melanoma tumor
   growth in syngeneic C57BL/6 mice is almost completely abolished. In
   contrast to apoptosis as the basic mechanism of the anticancer action of
   numerous chemotherapeutics, the important advantage of this SBA-15pSn
   mesoporous material is the induction of cell differentiation, an effect
   unknown for metal-based drugs and nanomaterials alone. This
   non-aggressive mode of drug action is highly efficient against cancer
   cells but is in the concentration range used nontoxic for normal tissue.
   JNK (Jun-amino-terminal kinase)-independent apoptosis accompanied by the
   development of the melanocyte-like nonproliferative phenotype of
   survived cells indicates the extraordinary potential of SBA-15pSn to
   suppress tumor growth without undesirable compensatory proliferation of
   malignant cells in response to neighboring cell death.",
journal = "Angewandte Chemie-International Edition",
title = "Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment",
number = "23",
volume = "53",
doi = "10.1002/anie.201400763",
pages = "5982-5987"
}

Bulatović, M. Z., Maksimović-Ivanić, D., Bensing, C., Gomez-Ruiz, S., Steinborn, D., Schmidt, H., Mojić, M., Korac, A., Golic, I., Perez-Quintanilla, D., Momčilović, M., Mijatović, S.,& Kaluđerović, G. N.. (2014). Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment. in Angewandte Chemie-International Edition, 53(23), 5982-5987.
https://doi.org/10.1002/anie.201400763

Bulatović MZ, Maksimović-Ivanić D, Bensing C, Gomez-Ruiz S, Steinborn D, Schmidt H, Mojić M, Korac A, Golic I, Perez-Quintanilla D, Momčilović M, Mijatović S, Kaluđerović GN. Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment. in Angewandte Chemie-International Edition. 2014;53(23):5982-5987.
doi:10.1002/anie.201400763 .

Bulatović, Mirna Z., Maksimović-Ivanić, Danijela, Bensing, Christian, Gomez-Ruiz, Santiago, Steinborn, Dirk, Schmidt, Harry, Mojić, Marija, Korac, Aleksandra, Golic, Igor, Perez-Quintanilla, Damian, Momčilović, Miljana, Mijatović, Sanja, Kaluđerović, Goran N., "Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment" in Angewandte Chemie-International Edition, 53, no. 23 (2014):5982-5987,
https://doi.org/10.1002/anie.201400763 . .

TY  - JOUR
AU  - Mojić, Marija
AU  - Bogdanović Pristov, Jelena
AU  - Maksimović-Ivanić, Danijela
AU  - Jones, David R.
AU  - Stanic, Marina
AU  - Mijatović, Sanja
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2177
AB  - In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.
T2  - Scientific Reports
T1  - Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study
IS  - 5955
VL  - 4
DO  - 10.1038/srep05955
ER  - 

@article{
author = "Mojić, Marija and Bogdanović Pristov, Jelena and Maksimović-Ivanić, Danijela and Jones, David R. and Stanic, Marina and Mijatović, Sanja and Spasojevic, Ivan",
year = "2014",
abstract = "In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.",
journal = "Scientific Reports",
title = "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study",
number = "5955",
volume = "4",
doi = "10.1038/srep05955"
}

Mojić, M., Bogdanović Pristov, J., Maksimović-Ivanić, D., Jones, D. R., Stanic, M., Mijatović, S.,& Spasojevic, I.. (2014). Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports, 4(5955).
https://doi.org/10.1038/srep05955

Mojić M, Bogdanović Pristov J, Maksimović-Ivanić D, Jones DR, Stanic M, Mijatović S, Spasojevic I. Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports. 2014;4(5955).
doi:10.1038/srep05955 .

Mojić, Marija, Bogdanović Pristov, Jelena, Maksimović-Ivanić, Danijela, Jones, David R., Stanic, Marina, Mijatović, Sanja, Spasojevic, Ivan, "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study" in Scientific Reports, 4, no. 5955 (2014),
https://doi.org/10.1038/srep05955 . .