TY  - JOUR
AU  - Vukšinović, Elvira L.
AU  - Pond, David W.
AU  - Grubor-Lajšić, Gordana
AU  - Worland, M. Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko D.
AU  - Blagojević, Duško
PY  - 2018
UR  - http://link.springer.com/10.1007/s00360-017-1110-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2777
AB  - Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.
T2  - Journal of Comparative Physiology B
T1  - Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls
VL  - 188
DO  - 10.1007/s00360-017-1110-9
SP  - 27
EP  - 36
ER  - 

@article{
author = "Vukšinović, Elvira L. and Pond, David W. and Grubor-Lajšić, Gordana and Worland, M. Roger and Kojić, Danijela and Purać, Jelena and Popović, Željko D. and Blagojević, Duško",
year = "2018",
abstract = "Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.",
journal = "Journal of Comparative Physiology B",
title = "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls",
volume = "188",
doi = "10.1007/s00360-017-1110-9",
pages = "27-36"
}

Vukšinović, E. L., Pond, D. W., Grubor-Lajšić, G., Worland, M. R., Kojić, D., Purać, J., Popović, Ž. D.,& Blagojević, D.. (2018). Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B, 188, 27-36.
https://doi.org/10.1007/s00360-017-1110-9

Vukšinović EL, Pond DW, Grubor-Lajšić G, Worland MR, Kojić D, Purać J, Popović ŽD, Blagojević D. Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B. 2018;188:27-36.
doi:10.1007/s00360-017-1110-9 .

Vukšinović, Elvira L., Pond, David W., Grubor-Lajšić, Gordana, Worland, M. Roger, Kojić, Danijela, Purać, Jelena, Popović, Željko D., Blagojević, Duško, "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls" in Journal of Comparative Physiology B, 188 (2018):27-36,
https://doi.org/10.1007/s00360-017-1110-9 . .

TY  - CHAP
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Petri, Edward
AU  - Popović, Željko D.
AU  - Grubor-Lajšić, Gordana
AU  - Blagojević, Duško
AU  - Raman, Chandrasekar
AU  - Goldsmith, Marian R.
AU  - Agunbiade, Tolulope A.
PY  - 2016
UR  - http://link.springer.com/10.1007/978-3-319-24244-6_4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2610
AB  - In this chapter, we review recent genomic, proteomic, and metabolomic studies that link several gene and protein products involved in cold adaptation in insects and other arthropods to energy metabolism and cellular protection mechanisms. Organisms have evolved various mechanisms for survival at subfreezing temperatures. In general, cold hardy invertebrates utilize four main strategies to survive cold temperatures: (1) freeze tolerance, (2) freeze avoidance, (3) cryoprotective dehydration, and (4) vitrification. In addition, many insects in temperate regions overwinter in an arrested developmental state known as diapause, during which they are cold hardy. Major alterations occur during winter diapause, with respect to both total metabolic flux and the relative activities of different metabolic pathways. In these organisms, one such metabolic adaptation to unfavorably cold environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The metabolic changes and metabolic paths involved in cold adaptation suggest involvement of specific enzymes and key regulatory proteins. These mechanisms of cold adaptation require precise scheduling of the expression of specific genes. Thus, we discuss here the evidence researchers have recently begun to gather supporting a relationship between the genes and proteins of the cold adaptation response and mechanisms of cellular protection and energy metabolism using an “omics” approach.
PB  - Springer International Publishing
T2  - Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2
T1  - Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach
DO  - 10.1007/978-3-319-24244-6_4
SP  - 89
EP  - 112
ER  - 

@inbook{
author = "Purać, Jelena and Kojić, Danijela and Petri, Edward and Popović, Željko D. and Grubor-Lajšić, Gordana and Blagojević, Duško and Raman, Chandrasekar and Goldsmith, Marian R. and Agunbiade, Tolulope A.",
year = "2016",
abstract = "In this chapter, we review recent genomic, proteomic, and metabolomic studies that link several gene and protein products involved in cold adaptation in insects and other arthropods to energy metabolism and cellular protection mechanisms. Organisms have evolved various mechanisms for survival at subfreezing temperatures. In general, cold hardy invertebrates utilize four main strategies to survive cold temperatures: (1) freeze tolerance, (2) freeze avoidance, (3) cryoprotective dehydration, and (4) vitrification. In addition, many insects in temperate regions overwinter in an arrested developmental state known as diapause, during which they are cold hardy. Major alterations occur during winter diapause, with respect to both total metabolic flux and the relative activities of different metabolic pathways. In these organisms, one such metabolic adaptation to unfavorably cold environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The metabolic changes and metabolic paths involved in cold adaptation suggest involvement of specific enzymes and key regulatory proteins. These mechanisms of cold adaptation require precise scheduling of the expression of specific genes. Thus, we discuss here the evidence researchers have recently begun to gather supporting a relationship between the genes and proteins of the cold adaptation response and mechanisms of cellular protection and energy metabolism using an “omics” approach.",
publisher = "Springer International Publishing",
journal = "Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2",
booktitle = "Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach",
doi = "10.1007/978-3-319-24244-6_4",
pages = "89-112"
}

Purać, J., Kojić, D., Petri, E., Popović, Ž. D., Grubor-Lajšić, G., Blagojević, D., Raman, C., Goldsmith, M. R.,& Agunbiade, T. A.. (2016). Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach. in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2
Springer International Publishing., 89-112.
https://doi.org/10.1007/978-3-319-24244-6_4

Purać J, Kojić D, Petri E, Popović ŽD, Grubor-Lajšić G, Blagojević D, Raman C, Goldsmith MR, Agunbiade TA. Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach. in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2. 2016;:89-112.
doi:10.1007/978-3-319-24244-6_4 .

Purać, Jelena, Kojić, Danijela, Petri, Edward, Popović, Željko D., Grubor-Lajšić, Gordana, Blagojević, Duško, Raman, Chandrasekar, Goldsmith, Marian R., Agunbiade, Tolulope A., "Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach" in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2 (2016):89-112,
https://doi.org/10.1007/978-3-319-24244-6_4 . .

TY  - JOUR
AU  - Popović, Željko
AU  - Subotić, Ana
AU  - Nikolić, Tatjana
AU  - Radojičić, Ratko
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
AU  - Koštál, Vladimír
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6336
AB  - Diapause is a state of arrested development during which insects cope with many external and internal stressful factors. European corn borer, Ostrinia nubilalis, overwinters as a fifth instar freeze-tolerant diapausing larva. In order to explore diapause-linked stress tolerance processes, the expression of selected genes coding for stress related proteins—glutathione S-transferase (Gst), thioredoxin (Trx), glutaredoxin (Grx), ferritin (Fer), metallothionein (Mtn), and heat shock proteins Hsp90, Hsc70, Hsp20.4, and Hsp20.1—was assessed in the fat body of diapause-destined, warm (22 °C) and cold (5 °C) acclimated diapausing larvae using the quantitative real-time PCR. Gene expression was normalised to mRNA transcripts for Actin and Rps03, and relative expression was calculated using non-diapausing larvae as a control group. During the initiation phase of diapause, the abundance of mRNA transcripts of Grx, Hsp90, Hsc70, andHsp20.1 was significantly upregulated, Trx, Fer, Mtn, andHsp20.1 were unchanged, while only Gst was clearly downregulated in comparison to non-diapause control. Later, in the early phase of diapause, the expression of most genes (except Trx and Hsp20.1) was upregulated in warm-acclimated
larvae, while only Trx and Hsp90 were upregulated in cold-acclimated larvae. Furthermore, the relative expression of all genes (except Trx) increased gradually throughout the diapause in cold-acclimated larvae. This result indicates that the half-life of mRNAs is prolonged during diapause at low temperature, which may lead to a gradual accumulation of mRNA transcripts. Our results show that both diapause programming and temperatures affect the expression of stress-related genes in Ostrinia nubilalis.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology, Part B
T1  - Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)
VL  - 186
DO  - 10.1016/j.cbpb.2015.04.004
SP  - 1
EP  - 7
ER  - 

@article{
author = "Popović, Željko and Subotić, Ana and Nikolić, Tatjana and Radojičić, Ratko and Blagojević, Duško and Grubor-Lajšić, Gordana and Koštál, Vladimír",
year = "2015",
abstract = "Diapause is a state of arrested development during which insects cope with many external and internal stressful factors. European corn borer, Ostrinia nubilalis, overwinters as a fifth instar freeze-tolerant diapausing larva. In order to explore diapause-linked stress tolerance processes, the expression of selected genes coding for stress related proteins—glutathione S-transferase (Gst), thioredoxin (Trx), glutaredoxin (Grx), ferritin (Fer), metallothionein (Mtn), and heat shock proteins Hsp90, Hsc70, Hsp20.4, and Hsp20.1—was assessed in the fat body of diapause-destined, warm (22 °C) and cold (5 °C) acclimated diapausing larvae using the quantitative real-time PCR. Gene expression was normalised to mRNA transcripts for Actin and Rps03, and relative expression was calculated using non-diapausing larvae as a control group. During the initiation phase of diapause, the abundance of mRNA transcripts of Grx, Hsp90, Hsc70, andHsp20.1 was significantly upregulated, Trx, Fer, Mtn, andHsp20.1 were unchanged, while only Gst was clearly downregulated in comparison to non-diapause control. Later, in the early phase of diapause, the expression of most genes (except Trx and Hsp20.1) was upregulated in warm-acclimated
larvae, while only Trx and Hsp90 were upregulated in cold-acclimated larvae. Furthermore, the relative expression of all genes (except Trx) increased gradually throughout the diapause in cold-acclimated larvae. This result indicates that the half-life of mRNAs is prolonged during diapause at low temperature, which may lead to a gradual accumulation of mRNA transcripts. Our results show that both diapause programming and temperatures affect the expression of stress-related genes in Ostrinia nubilalis.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology, Part B",
title = "Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)",
volume = "186",
doi = "10.1016/j.cbpb.2015.04.004",
pages = "1-7"
}

Popović, Ž., Subotić, A., Nikolić, T., Radojičić, R., Blagojević, D., Grubor-Lajšić, G.,& Koštál, V.. (2015). Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.). in Comparative Biochemistry and Physiology, Part B
Elsevier Inc.., 186, 1-7.
https://doi.org/10.1016/j.cbpb.2015.04.004

Popović Ž, Subotić A, Nikolić T, Radojičić R, Blagojević D, Grubor-Lajšić G, Koštál V. Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.). in Comparative Biochemistry and Physiology, Part B. 2015;186:1-7.
doi:10.1016/j.cbpb.2015.04.004 .

Popović, Željko, Subotić, Ana, Nikolić, Tatjana, Radojičić, Ratko, Blagojević, Duško, Grubor-Lajšić, Gordana, Koštál, Vladimír, "Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)" in Comparative Biochemistry and Physiology, Part B, 186 (2015):1-7,
https://doi.org/10.1016/j.cbpb.2015.04.004 . .

TY  - JOUR
AU  - Grubor-Lajšić, Gordana
AU  - Petri, Edward
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko
AU  - Worland, Michael Roger
AU  - Clark, Melody S.
AU  - Mojović, Miloš
AU  - Blagojević, Duško
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6338
AB  - The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.
PB  - Hoboken: John Wiley and Sons
T2  - Archives of Insect Biochemistry and Physiology
T1  - Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)
IS  - 2
VL  - 82
DO  - 10.1002/arch.21073
SP  - 59
EP  - 70
ER  - 

@article{
author = "Grubor-Lajšić, Gordana and Petri, Edward and Kojić, Danijela and Purać, Jelena and Popović, Željko and Worland, Michael Roger and Clark, Melody S. and Mojović, Miloš and Blagojević, Duško",
year = "2013",
abstract = "The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Archives of Insect Biochemistry and Physiology",
title = "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)",
number = "2",
volume = "82",
doi = "10.1002/arch.21073",
pages = "59-70"
}

Grubor-Lajšić, G., Petri, E., Kojić, D., Purać, J., Popović, Ž., Worland, M. R., Clark, M. S., Mojović, M.,& Blagojević, D.. (2013). Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology
Hoboken: John Wiley and Sons., 82(2), 59-70.
https://doi.org/10.1002/arch.21073

Grubor-Lajšić G, Petri E, Kojić D, Purać J, Popović Ž, Worland MR, Clark MS, Mojović M, Blagojević D. Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology. 2013;82(2):59-70.
doi:10.1002/arch.21073 .

Grubor-Lajšić, Gordana, Petri, Edward, Kojić, Danijela, Purać, Jelena, Popović, Željko, Worland, Michael Roger, Clark, Melody S., Mojović, Miloš, Blagojević, Duško, "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)" in Archives of Insect Biochemistry and Physiology, 82, no. 2 (2013):59-70,
https://doi.org/10.1002/arch.21073 . .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Pond, David
AU  - Worland, Michael Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6337
AB  - This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
T1  - Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)
IS  - 4
VL  - 165
DO  - 10.1016/j.cbpb.2013.05.001
SP  - 219
EP  - 225
ER  - 

@article{
author = "Vukašinović, Elvira and Pond, David and Worland, Michael Roger and Kojić, Danijela and Purać, Jelena and Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2013",
abstract = "This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology",
title = "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)",
number = "4",
volume = "165",
doi = "10.1016/j.cbpb.2013.05.001",
pages = "219-225"
}

Vukašinović, E., Pond, D., Worland, M. R., Kojić, D., Purać, J., Blagojević, D.,& Grubor-Lajšić, G.. (2013). Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Elsevier Inc.., 165(4), 219-225.
https://doi.org/10.1016/j.cbpb.2013.05.001

Vukašinović E, Pond D, Worland MR, Kojić D, Purać J, Blagojević D, Grubor-Lajšić G. Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2013;165(4):219-225.
doi:10.1016/j.cbpb.2013.05.001 .

Vukašinović, Elvira, Pond, David, Worland, Michael Roger, Kojić, Danijela, Purać, Jelena, Blagojević, Duško, Grubor-Lajšić, Gordana, "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)" in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 165, no. 4 (2013):219-225,
https://doi.org/10.1016/j.cbpb.2013.05.001 . .

TY  - JOUR
AU  - Stankov, Karmen
AU  - Bogdanović, Gordana M
AU  - Stankov, Suncica
AU  - Drasković, Dragan O
AU  - Grubor-Lajšić, Gordana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1187
AB  - Purpose: Activation of T cells by direct stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io) results in numerous downstream signals that activate pathways enabling T cells to proliferate and produce cytokines. Inducible T cell activation is regulated predominantly at the transcriptional level. Therefore, we were interested to analyze the transcriptional activity of the 19 genes involved in the regulation of several important cellular processes. Methods: Quantitative real-time (RT) PCR analysis was performed using mRNA-specific primers and SybrGreen for relative mRNA expression levels of all the examined genes. Results: Our results showed c-kit expression in Jurkat cells, further confirmed by sequencing of c-kit mRNA-specific PCR product. The expected increased expression of interleukin (IL)-2 mRNA, together with moderate Ki-67 upregulation, indicate the proliferation of PMA/Io treated Jurkat cells. Significant upregulation of nuclear factor (NF)-kappa B, JNK and the prosurvival Bcl-2 was followed by activation of only one protein kinase RNA-like endoplasmic reticulum kinase (PERK) out of 3 main endoplasmic reticulum (ER) stress subpathways (ATF6 and spliced XBP were downregulated). NF-kappa B and JNK activation, as well as ERK down regulation were reactive oxygen species (ROS)-independent, shown by the lack of activation of antioxidative enzymes (SOD, NOS, GSTP 1, gGCS and GR). C-kit was downregulated in the absence of exogenous SCF (c-kit ligand). Conclusion: Based on these data it is concluded that the PMA/Io treatment of Jurkat cells induced increased expression of IL-2. followed by upregulation of prosurvival genes belonging to the Bcl-2 family. Neither c-kit nor the antioxidative system were activated, excluding their role in Jurkat T-cell activation in the absence of exogenous c-kit ligand SCF.
T2  - Journal of Buon
T1  - Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells
IS  - 2
VL  - 17
EP  - 376
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1187
ER  - 

@article{
author = "Stankov, Karmen and Bogdanović, Gordana M and Stankov, Suncica and Drasković, Dragan O and Grubor-Lajšić, Gordana and Spasić, Mihajlo and Blagojević, Duško",
year = "2012",
abstract = "Purpose: Activation of T cells by direct stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io) results in numerous downstream signals that activate pathways enabling T cells to proliferate and produce cytokines. Inducible T cell activation is regulated predominantly at the transcriptional level. Therefore, we were interested to analyze the transcriptional activity of the 19 genes involved in the regulation of several important cellular processes. Methods: Quantitative real-time (RT) PCR analysis was performed using mRNA-specific primers and SybrGreen for relative mRNA expression levels of all the examined genes. Results: Our results showed c-kit expression in Jurkat cells, further confirmed by sequencing of c-kit mRNA-specific PCR product. The expected increased expression of interleukin (IL)-2 mRNA, together with moderate Ki-67 upregulation, indicate the proliferation of PMA/Io treated Jurkat cells. Significant upregulation of nuclear factor (NF)-kappa B, JNK and the prosurvival Bcl-2 was followed by activation of only one protein kinase RNA-like endoplasmic reticulum kinase (PERK) out of 3 main endoplasmic reticulum (ER) stress subpathways (ATF6 and spliced XBP were downregulated). NF-kappa B and JNK activation, as well as ERK down regulation were reactive oxygen species (ROS)-independent, shown by the lack of activation of antioxidative enzymes (SOD, NOS, GSTP 1, gGCS and GR). C-kit was downregulated in the absence of exogenous SCF (c-kit ligand). Conclusion: Based on these data it is concluded that the PMA/Io treatment of Jurkat cells induced increased expression of IL-2. followed by upregulation of prosurvival genes belonging to the Bcl-2 family. Neither c-kit nor the antioxidative system were activated, excluding their role in Jurkat T-cell activation in the absence of exogenous c-kit ligand SCF.",
journal = "Journal of Buon",
title = "Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells",
number = "2",
volume = "17",
pages = "376",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1187"
}

Stankov, K., Bogdanović, G. M., Stankov, S., Drasković, D. O., Grubor-Lajšić, G., Spasić, M.,& Blagojević, D.. (2012). Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells. in Journal of Buon, 17(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1187

Stankov K, Bogdanović GM, Stankov S, Drasković DO, Grubor-Lajšić G, Spasić M, Blagojević D. Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells. in Journal of Buon. 2012;17(2):null-376.
https://hdl.handle.net/21.15107/rcub_ibiss_1187 .

Stankov, Karmen, Bogdanović, Gordana M, Stankov, Suncica, Drasković, Dragan O, Grubor-Lajšić, Gordana, Spasić, Mihajlo, Blagojević, Duško, "Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells" in Journal of Buon, 17, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1187 .

TY  - JOUR
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
AU  - Spasić, Mihajlo
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4053
AB  - Low temperatures provoke increased production of heat accompanied by increased respiration, oxygen consumption and the production of partially reduced oxygen species called ROS. ROS induce different forms of cellular oxidative damage, disturb the redox state and can change the activity of several metabolic enzymes. Organisms have developed a functionally connected set of anti-oxidant enzymes and low molecular mass compounds (together termed the ADS) that metabolise primary ROS. If ROS production within cells overwhelms the ADS, oxidative damage arises and oxidative stress can occur. Short-term cold exposure in endotherms leads to oxidative stress. As cold exposure persists organisms develop adaptive changes toward reducing ROS production and increasing the ADS. In contrast, heterotherms and ectotherms as a normal part of their over-wintering strategy slow down metabolism, oxygen consumption and subsequently cause ROS production. Increased baseline activity of key anti-oxidant enzymes as well as 'secondary' enzymatic defence and/or glutathione levels in preparation for a putative oxidative stressful situation arising from tissue re-oxygenation seems to be the preferred evolutionary adaptation of such animals exposed to low environmental temperatures.
PB  - Frontiers in Bioscience
T2  - Frontiers in bioscience (Scholar edition)
T1  - Cold defence responses: The role of oxidative stress
IS  - 2
VL  - 3
DO  - 10.2741/s161
SP  - 416
EP  - 417
ER  - 

@article{
author = "Blagojević, Duško and Grubor-Lajšić, Gordana and Spasić, Mihajlo",
year = "2011",
abstract = "Low temperatures provoke increased production of heat accompanied by increased respiration, oxygen consumption and the production of partially reduced oxygen species called ROS. ROS induce different forms of cellular oxidative damage, disturb the redox state and can change the activity of several metabolic enzymes. Organisms have developed a functionally connected set of anti-oxidant enzymes and low molecular mass compounds (together termed the ADS) that metabolise primary ROS. If ROS production within cells overwhelms the ADS, oxidative damage arises and oxidative stress can occur. Short-term cold exposure in endotherms leads to oxidative stress. As cold exposure persists organisms develop adaptive changes toward reducing ROS production and increasing the ADS. In contrast, heterotherms and ectotherms as a normal part of their over-wintering strategy slow down metabolism, oxygen consumption and subsequently cause ROS production. Increased baseline activity of key anti-oxidant enzymes as well as 'secondary' enzymatic defence and/or glutathione levels in preparation for a putative oxidative stressful situation arising from tissue re-oxygenation seems to be the preferred evolutionary adaptation of such animals exposed to low environmental temperatures.",
publisher = "Frontiers in Bioscience",
journal = "Frontiers in bioscience (Scholar edition)",
title = "Cold defence responses: The role of oxidative stress",
number = "2",
volume = "3",
doi = "10.2741/s161",
pages = "416-417"
}

Blagojević, D., Grubor-Lajšić, G.,& Spasić, M.. (2011). Cold defence responses: The role of oxidative stress. in Frontiers in bioscience (Scholar edition)
Frontiers in Bioscience., 3(2), 416-417.
https://doi.org/10.2741/s161

Blagojević D, Grubor-Lajšić G, Spasić M. Cold defence responses: The role of oxidative stress. in Frontiers in bioscience (Scholar edition). 2011;3(2):416-417.
doi:10.2741/s161 .

Blagojević, Duško, Grubor-Lajšić, Gordana, Spasić, Mihajlo, "Cold defence responses: The role of oxidative stress" in Frontiers in bioscience (Scholar edition), 3, no. 2 (2011):416-417,
https://doi.org/10.2741/s161 . .

TY  - JOUR
AU  - Popović, Ž.D.
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Pamer, Elvira L.
AU  - Worland, M.R.
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/282
AB  - The Arctic springtail Megaphorura arctica (Tullberg, 1876) employs a strategy known as cryoprotective dehydration to survive winter temperatures as low as -25°C. During cryoprotective dehydration, water is lost from the animal to ice in its surroundings as a result of the difference in vapour pressure between the animal's supercooled body fluids and ice (Worland et al., 1998; Holmstrup and Somme, 1998). This mechanism ensures that as the habitat temperature falls, the concentration of solutes remains high enough to prevent freezing (Holmstrup et al., 2002). In M. arctica, accumulation of trehalose, a cryo/anhydro protectant, occurs in parallel with dehydration. Recent studies have identified a number of genes and cellular processes involved in cryoprotective dehydration in M. arctica (Clark et al., 2007; Clark et al., 2009; Purać et al., 2011). One of them includes late embryogenesis abundant (LEA) proteins. This study, together with that of Bahrndorff et al. (2008), suggests that LEA proteins may be involved in protective dehydration in this species.
T2  - Archives of Biological Sciences
T1  - Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica
IS  - 3
VL  - 63
SP  - 681
EP  - 683
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_282
ER  - 

@article{
author = "Popović, Ž.D. and Purać, Jelena and Kojić, Danijela and Pamer, Elvira L. and Worland, M.R. and Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2011, 2011",
abstract = "The Arctic springtail Megaphorura arctica (Tullberg, 1876) employs a strategy known as cryoprotective dehydration to survive winter temperatures as low as -25°C. During cryoprotective dehydration, water is lost from the animal to ice in its surroundings as a result of the difference in vapour pressure between the animal's supercooled body fluids and ice (Worland et al., 1998; Holmstrup and Somme, 1998). This mechanism ensures that as the habitat temperature falls, the concentration of solutes remains high enough to prevent freezing (Holmstrup et al., 2002). In M. arctica, accumulation of trehalose, a cryo/anhydro protectant, occurs in parallel with dehydration. Recent studies have identified a number of genes and cellular processes involved in cryoprotective dehydration in M. arctica (Clark et al., 2007; Clark et al., 2009; Purać et al., 2011). One of them includes late embryogenesis abundant (LEA) proteins. This study, together with that of Bahrndorff et al. (2008), suggests that LEA proteins may be involved in protective dehydration in this species.",
journal = "Archives of Biological Sciences",
title = "Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica",
number = "3",
volume = "63",
pages = "681-683",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_282"
}

Popović, Ž.D., Purać, J., Kojić, D., Pamer, E. L., Worland, M.R., Blagojević, D.,& Grubor-Lajšić, G.. (2011). Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica. in Archives of Biological Sciences, 63(3), 681-683.
https://hdl.handle.net/21.15107/rcub_ibiss_282

Popović Ž, Purać J, Kojić D, Pamer EL, Worland M, Blagojević D, Grubor-Lajšić G. Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica. in Archives of Biological Sciences. 2011;63(3):681-683.
https://hdl.handle.net/21.15107/rcub_ibiss_282 .

Popović, Ž.D., Purać, Jelena, Kojić, Danijela, Pamer, Elvira L., Worland, M.R., Blagojević, Duško, Grubor-Lajšić, Gordana, "Lea protein expression during cold-induced dehydration in the Arctic collembola Megaphorura arctica" in Archives of Biological Sciences, 63, no. 3 (2011):681-683,
https://hdl.handle.net/21.15107/rcub_ibiss_282 .

TY  - JOUR
AU  - Purac, Jelena
AU  - Pond, David W
AU  - Grubor-Lajšić, Gordana
AU  - Kojić, Danijela K
AU  - Blagojević, Duško
AU  - Worland, Roger M
AU  - Clark, Melody S
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1288
AB  - Cold hardiness in the Arctic Collembola Megaphorura arctica (Tullberg), formerly Onychiurus arcticus, has been the subject of extensive studies over the last decade. This species employs an unusual strategy known as cryoprotective dehydration to survive winter temperatures as low as -25 degrees C. To expand knowledge of cryoprotective dehydration in M. arctica, the present study investigates how a reduction in ambient temperature affects the fatty acid composition of the total body lipid content along with polar (mainly membrane phospholipids) and nonpolar (mainly triacylglycerols) lipids. Most ectothermic animals compensate for changes in fluidity by regulating fatty acid composition, a process often described as homeoviscous adaptation. In M. arctica, changes in the fatty acid composition of total body lipid content during cold treatment are only moderate, with no clear pattern emerging. However, the levels of unsaturated fatty acids in the polar lipids increase with cold exposure, largely attributable to 16 : 1(n - 7), 18 : 1(n - 9), 18 : 3(n - 6) and 18 : 3(n - 3), whereas unsaturated fatty acid levels in the nonpolar lipids correspondingly decrease. These results suggest a reallocation of fatty acids between the two lipid pools as a response to a temperature reduction of 6 degrees C. Because of hypometabolism, a characteristic of cold adaptation, such a mechanism could be less energy demanding than de novo synthesis of fatty acids and may comprise part of an adaptive homeostatic response.
T2  - Physiological Entomology
T1  - Cold hardening induces transfer of fatty acids between polar and nonpolar lipid pools in the Arctic collembollan Megaphorura arctica
IS  - 2
VL  - 36
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1288
ER  - 

@article{
author = "Purac, Jelena and Pond, David W and Grubor-Lajšić, Gordana and Kojić, Danijela K and Blagojević, Duško and Worland, Roger M and Clark, Melody S",
year = "2011",
abstract = "Cold hardiness in the Arctic Collembola Megaphorura arctica (Tullberg), formerly Onychiurus arcticus, has been the subject of extensive studies over the last decade. This species employs an unusual strategy known as cryoprotective dehydration to survive winter temperatures as low as -25 degrees C. To expand knowledge of cryoprotective dehydration in M. arctica, the present study investigates how a reduction in ambient temperature affects the fatty acid composition of the total body lipid content along with polar (mainly membrane phospholipids) and nonpolar (mainly triacylglycerols) lipids. Most ectothermic animals compensate for changes in fluidity by regulating fatty acid composition, a process often described as homeoviscous adaptation. In M. arctica, changes in the fatty acid composition of total body lipid content during cold treatment are only moderate, with no clear pattern emerging. However, the levels of unsaturated fatty acids in the polar lipids increase with cold exposure, largely attributable to 16 : 1(n - 7), 18 : 1(n - 9), 18 : 3(n - 6) and 18 : 3(n - 3), whereas unsaturated fatty acid levels in the nonpolar lipids correspondingly decrease. These results suggest a reallocation of fatty acids between the two lipid pools as a response to a temperature reduction of 6 degrees C. Because of hypometabolism, a characteristic of cold adaptation, such a mechanism could be less energy demanding than de novo synthesis of fatty acids and may comprise part of an adaptive homeostatic response.",
journal = "Physiological Entomology",
title = "Cold hardening induces transfer of fatty acids between polar and nonpolar lipid pools in the Arctic collembollan Megaphorura arctica",
number = "2",
volume = "36",
pages = "140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1288"
}

Purac, J., Pond, D. W., Grubor-Lajšić, G., Kojić, D. K., Blagojević, D., Worland, R. M.,& Clark, M. S.. (2011). Cold hardening induces transfer of fatty acids between polar and nonpolar lipid pools in the Arctic collembollan Megaphorura arctica. in Physiological Entomology, 36(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1288

Purac J, Pond DW, Grubor-Lajšić G, Kojić DK, Blagojević D, Worland RM, Clark MS. Cold hardening induces transfer of fatty acids between polar and nonpolar lipid pools in the Arctic collembollan Megaphorura arctica. in Physiological Entomology. 2011;36(2):null-140.
https://hdl.handle.net/21.15107/rcub_ibiss_1288 .

Purac, Jelena, Pond, David W, Grubor-Lajšić, Gordana, Kojić, Danijela K, Blagojević, Duško, Worland, Roger M, Clark, Melody S, "Cold hardening induces transfer of fatty acids between polar and nonpolar lipid pools in the Arctic collembollan Megaphorura arctica" in Physiological Entomology, 36, no. 2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1288 .

TY  - JOUR
AU  - Kojić, Danijela K
AU  - Spasojević, Ivan B
AU  - Mojović, Milos D
AU  - Blagojević, Duško
AU  - Worland, Roger M
AU  - Grubor-Lajšić, Gordana
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1471
AB  - The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H(2)O(2)), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H(2)O(2) concentration was recorded. A possible regulatory role of H(2)O(2) at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.
T2  - European Journal of Entomology
T1  - Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
IS  - 3
VL  - 106
EP  - 454
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1471
ER  - 

@article{
author = "Kojić, Danijela K and Spasojević, Ivan B and Mojović, Milos D and Blagojević, Duško and Worland, Roger M and Grubor-Lajšić, Gordana and Spasić, Mihajlo",
year = "2009",
abstract = "The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H(2)O(2)), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H(2)O(2) concentration was recorded. A possible regulatory role of H(2)O(2) at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.",
journal = "European Journal of Entomology",
title = "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)",
number = "3",
volume = "106",
pages = "454",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1471"
}

Kojić, D. K., Spasojević, I. B., Mojović, M. D., Blagojević, D., Worland, R. M., Grubor-Lajšić, G.,& Spasić, M.. (2009). Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology, 106(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1471

Kojić DK, Spasojević IB, Mojović MD, Blagojević D, Worland RM, Grubor-Lajšić G, Spasić M. Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology. 2009;106(3):null-454.
https://hdl.handle.net/21.15107/rcub_ibiss_1471 .

Kojić, Danijela K, Spasojević, Ivan B, Mojović, Milos D, Blagojević, Duško, Worland, Roger M, Grubor-Lajšić, Gordana, Spasić, Mihajlo, "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)" in European Journal of Entomology, 106, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1471 .

TY  - JOUR
AU  - Stanić, Bojana
AU  - Jovanović-Galović, Aleksandra
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
AU  - Worland, Roger
AU  - Spasić, Mihajlo
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1752
AB  - Many insects in temperate regions overwinter in diapause, during which they are cold hardy. In these insects, one of the metabolic adaptations to the unfavorable environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The aim of this study was to investigate the connection between the antioxidative system and synthesis of cryoprotectants (mainly glycerol) in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae). At two periods during diapause (November and February), in three groups of insects (kept under field conditions; -12degreesC for two weeks; 8degreesC for two weeks), the activity of key enzymes of the antioxidative system and oxidative part of the hexose monophosphate shunt were measured: superoxide dismutase, catalase, non selenium glutathione peroxidase, glutathione S-transferase, glutathione reductase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, as well that of the antioxidative components: total glutathione and ascorbate, and dehydroascorbate reductase. There was a higher activity of antioxidative enzymes at the beginning of the diapause period (November) compared to late diapause (February), while glutathione and ascorbate were higher in February. Similarly, there was a lower activity of the hexose monophosphate shunt enzymes in February. Exposure of larvae to -12degreesC resulted in an elevation of hexose monophosphate shunt enzyme activity, especially in November. This was accompanied by a significant increase in glycerol content in February. Changes in ascorbate levels and dehydroascorbate reductase activity in both experimental groups (-12degreesC and 8degreesC) suggest a connection between the antioxidative system, metabolism during diapause and cold hardiness. Our results support the notion that antioxidative defense in larvae of Ostrinia nubilalis is closely connected with metabolic changes characteristic of diapause, mechanisms of cold hardiness involved in diapause and the maintenance of a stable redox state.
T2  - European Journal of Entomology
T1  - Cold hardiness in Ostrinia nubilalis (Lepidoptera : Pyralidae): Glycerol content, hexose monophosphate shunt activity, and antioxidative defense system
IS  - 3
VL  - 101
DO  - 10.14411/eje.2004.065
SP  - 459
EP  - 466
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1752
ER  - 

@article{
author = "Stanić, Bojana and Jovanović-Galović, Aleksandra and Blagojević, Duško and Grubor-Lajšić, Gordana and Worland, Roger and Spasić, Mihajlo",
year = "2004",
abstract = "Many insects in temperate regions overwinter in diapause, during which they are cold hardy. In these insects, one of the metabolic adaptations to the unfavorable environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The aim of this study was to investigate the connection between the antioxidative system and synthesis of cryoprotectants (mainly glycerol) in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae). At two periods during diapause (November and February), in three groups of insects (kept under field conditions; -12degreesC for two weeks; 8degreesC for two weeks), the activity of key enzymes of the antioxidative system and oxidative part of the hexose monophosphate shunt were measured: superoxide dismutase, catalase, non selenium glutathione peroxidase, glutathione S-transferase, glutathione reductase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, as well that of the antioxidative components: total glutathione and ascorbate, and dehydroascorbate reductase. There was a higher activity of antioxidative enzymes at the beginning of the diapause period (November) compared to late diapause (February), while glutathione and ascorbate were higher in February. Similarly, there was a lower activity of the hexose monophosphate shunt enzymes in February. Exposure of larvae to -12degreesC resulted in an elevation of hexose monophosphate shunt enzyme activity, especially in November. This was accompanied by a significant increase in glycerol content in February. Changes in ascorbate levels and dehydroascorbate reductase activity in both experimental groups (-12degreesC and 8degreesC) suggest a connection between the antioxidative system, metabolism during diapause and cold hardiness. Our results support the notion that antioxidative defense in larvae of Ostrinia nubilalis is closely connected with metabolic changes characteristic of diapause, mechanisms of cold hardiness involved in diapause and the maintenance of a stable redox state.",
journal = "European Journal of Entomology",
title = "Cold hardiness in Ostrinia nubilalis (Lepidoptera : Pyralidae): Glycerol content, hexose monophosphate shunt activity, and antioxidative defense system",
number = "3",
volume = "101",
doi = "10.14411/eje.2004.065",
pages = "459-466",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1752"
}

Stanić, B., Jovanović-Galović, A., Blagojević, D., Grubor-Lajšić, G., Worland, R.,& Spasić, M.. (2004). Cold hardiness in Ostrinia nubilalis (Lepidoptera : Pyralidae): Glycerol content, hexose monophosphate shunt activity, and antioxidative defense system. in European Journal of Entomology, 101(3), 459-466.
https://doi.org/10.14411/eje.2004.065
https://hdl.handle.net/21.15107/rcub_ibiss_1752

Stanić B, Jovanović-Galović A, Blagojević D, Grubor-Lajšić G, Worland R, Spasić M. Cold hardiness in Ostrinia nubilalis (Lepidoptera : Pyralidae): Glycerol content, hexose monophosphate shunt activity, and antioxidative defense system. in European Journal of Entomology. 2004;101(3):459-466.
doi:10.14411/eje.2004.065
https://hdl.handle.net/21.15107/rcub_ibiss_1752 .

Stanić, Bojana, Jovanović-Galović, Aleksandra, Blagojević, Duško, Grubor-Lajšić, Gordana, Worland, Roger, Spasić, Mihajlo, "Cold hardiness in Ostrinia nubilalis (Lepidoptera : Pyralidae): Glycerol content, hexose monophosphate shunt activity, and antioxidative defense system" in European Journal of Entomology, 101, no. 3 (2004):459-466,
https://doi.org/10.14411/eje.2004.065 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1752 .

TY  - JOUR
AU  - Pavlović, Slađan
AU  - Belić, Dragana
AU  - Blagojević, Duško
AU  - Radojičić, Ratko M.
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
AU  - Grubor-Lajšić, Gordana
AU  - Spasić, Mihajlo
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1683
AB  - The activity of cytosolic antioxidative defence enzymes in the liver and white muscle of thinlip gray mullet (Liza ramada Risso) were compared in winter and spring in the Adriatic Sea.. Activity of antioxidative enzymes is functionally organized due to metabolic demands: analyses of variance and correlation analysis revealed tissue- and seasonal- specific organization of antioxidative enzymes. In winter GST activity increased in both tissues compared with spring. At the same time decreased GSH-Px and GR activities were observed and this effect was more pronounced in liver then in white muscle. From correlation analyses it is concluded that the antioxidative components correlate, but the composition of the antioxidative defence system is different in respect to season and tissue. This means that the antioxidative defence system reorganizes its structure due to oxidative demands and to protect the tissues against reactive oxygen species and to establish homeostasis. Discriminant analyses separated groups according to the complete organization of individual components of the system very well and identified individual components (CAT, GST and GR) which contribute most to the differences. Statistical differences were observed between enzyme activities in tissues (liver and muscle) in both winter and spring, and between seasons (winter and spring) for liver tissue only. Since environmental parameters, such as temperature and oxygen concentration in the sea differ with season, we conclude that in this species the tissues examined expressed their antioxidative defence systems in different ways in respect of external/environmental conditions. We propose that tissue- and seasonal- specific levels of antioxidant enzyme activities should be considered in the interpretation of data from future biomonitoring field studies, especially in relation to low temperature.
T2  - Cryoletters
T1  - Seasonal variations of cytosolic antioxidant enzyme activities in the liver and white muscle of thinlip gray mullet (Liza ramada Risso) from the adriatic sea
IS  - 4
VL  - 25
EP  - 285
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1683
ER  - 

@article{
author = "Pavlović, Slađan and Belić, Dragana and Blagojević, Duško and Radojičić, Ratko M. and Žikić, Radoslav V. and Saičić, Zorica and Grubor-Lajšić, Gordana and Spasić, Mihajlo",
year = "2004",
abstract = "The activity of cytosolic antioxidative defence enzymes in the liver and white muscle of thinlip gray mullet (Liza ramada Risso) were compared in winter and spring in the Adriatic Sea.. Activity of antioxidative enzymes is functionally organized due to metabolic demands: analyses of variance and correlation analysis revealed tissue- and seasonal- specific organization of antioxidative enzymes. In winter GST activity increased in both tissues compared with spring. At the same time decreased GSH-Px and GR activities were observed and this effect was more pronounced in liver then in white muscle. From correlation analyses it is concluded that the antioxidative components correlate, but the composition of the antioxidative defence system is different in respect to season and tissue. This means that the antioxidative defence system reorganizes its structure due to oxidative demands and to protect the tissues against reactive oxygen species and to establish homeostasis. Discriminant analyses separated groups according to the complete organization of individual components of the system very well and identified individual components (CAT, GST and GR) which contribute most to the differences. Statistical differences were observed between enzyme activities in tissues (liver and muscle) in both winter and spring, and between seasons (winter and spring) for liver tissue only. Since environmental parameters, such as temperature and oxygen concentration in the sea differ with season, we conclude that in this species the tissues examined expressed their antioxidative defence systems in different ways in respect of external/environmental conditions. We propose that tissue- and seasonal- specific levels of antioxidant enzyme activities should be considered in the interpretation of data from future biomonitoring field studies, especially in relation to low temperature.",
journal = "Cryoletters",
title = "Seasonal variations of cytosolic antioxidant enzyme activities in the liver and white muscle of thinlip gray mullet (Liza ramada Risso) from the adriatic sea",
number = "4",
volume = "25",
pages = "285",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1683"
}

Pavlović, S., Belić, D., Blagojević, D., Radojičić, R. M., Žikić, R. V., Saičić, Z., Grubor-Lajšić, G.,& Spasić, M.. (2004). Seasonal variations of cytosolic antioxidant enzyme activities in the liver and white muscle of thinlip gray mullet (Liza ramada Risso) from the adriatic sea. in Cryoletters, 25(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1683

Pavlović S, Belić D, Blagojević D, Radojičić RM, Žikić RV, Saičić Z, Grubor-Lajšić G, Spasić M. Seasonal variations of cytosolic antioxidant enzyme activities in the liver and white muscle of thinlip gray mullet (Liza ramada Risso) from the adriatic sea. in Cryoletters. 2004;25(4):null-285.
https://hdl.handle.net/21.15107/rcub_ibiss_1683 .

Pavlović, Slađan, Belić, Dragana, Blagojević, Duško, Radojičić, Ratko M., Žikić, Radoslav V., Saičić, Zorica, Grubor-Lajšić, Gordana, Spasić, Mihajlo, "Seasonal variations of cytosolic antioxidant enzyme activities in the liver and white muscle of thinlip gray mullet (Liza ramada Risso) from the adriatic sea" in Cryoletters, 25, no. 4 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1683 .

TY  - JOUR
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2000
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/68
AB  - Amount of reactive oxygen species (ROS) produced in tissues of vertebrates is regulated by their elimination through coordinate action and level of enzymatic (SODs - superoxide dismutases, CAT-catalase, GSH-Px - glutathione peroxidase, GSTs - glutathione-S-transferases and GR - glutathione reductase) and non-enzymatic (glutahione, ascorbate and tocopherols) antioxidant (AO) components. Different from vertebrates, incest species lack selenium-dependent GSH-Px and posses active enzymatic ascorbate recycling pathway serving to eliminate hydrogen peroxide. Moreover, the sites of ROS generation in insect cells are compartmentalized and dislocated within the cells. Consequently, AO components are widely distributed at subcellular level. The mode of the regulation of AO components functioning in insects has been studied in diverse insect species employing different agents acting as inducers of oxidative stress, as well as during reproduction, development and ageing. The results reported so far showed that incest AO system is very flexible and dynamically organized. Besides, it has been demonstrated that this system operates not only to eliminate extensive ROS but also to recompose particular ROS which express direct physiological effects (e.g. hydrogen peroxide and nitric oxide) and to establish optimal redox state.
T2  - Archives of Biological Sciences
T1  - Multifunctionality of antioxidant system in insects
IS  - 4
VL  - 52
SP  - 185
EP  - 194
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_68
ER  - 

@article{
author = "Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2000, 2000",
abstract = "Amount of reactive oxygen species (ROS) produced in tissues of vertebrates is regulated by their elimination through coordinate action and level of enzymatic (SODs - superoxide dismutases, CAT-catalase, GSH-Px - glutathione peroxidase, GSTs - glutathione-S-transferases and GR - glutathione reductase) and non-enzymatic (glutahione, ascorbate and tocopherols) antioxidant (AO) components. Different from vertebrates, incest species lack selenium-dependent GSH-Px and posses active enzymatic ascorbate recycling pathway serving to eliminate hydrogen peroxide. Moreover, the sites of ROS generation in insect cells are compartmentalized and dislocated within the cells. Consequently, AO components are widely distributed at subcellular level. The mode of the regulation of AO components functioning in insects has been studied in diverse insect species employing different agents acting as inducers of oxidative stress, as well as during reproduction, development and ageing. The results reported so far showed that incest AO system is very flexible and dynamically organized. Besides, it has been demonstrated that this system operates not only to eliminate extensive ROS but also to recompose particular ROS which express direct physiological effects (e.g. hydrogen peroxide and nitric oxide) and to establish optimal redox state.",
journal = "Archives of Biological Sciences",
title = "Multifunctionality of antioxidant system in insects",
number = "4",
volume = "52",
pages = "185-194",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_68"
}

Blagojević, D.,& Grubor-Lajšić, G.. (2000). Multifunctionality of antioxidant system in insects. in Archives of Biological Sciences, 52(4), 185-194.
https://hdl.handle.net/21.15107/rcub_ibiss_68

Blagojević D, Grubor-Lajšić G. Multifunctionality of antioxidant system in insects. in Archives of Biological Sciences. 2000;52(4):185-194.
https://hdl.handle.net/21.15107/rcub_ibiss_68 .

Blagojević, Duško, Grubor-Lajšić, Gordana, "Multifunctionality of antioxidant system in insects" in Archives of Biological Sciences, 52, no. 4 (2000):185-194,
https://hdl.handle.net/21.15107/rcub_ibiss_68 .