TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6453
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
IS  - 1
VL  - 13
DO  - 10.3390/antibiotics13010041
SP  - 41
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
number = "1",
volume = "13",
doi = "10.3390/antibiotics13010041",
pages = "41"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
Basel: MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Marlani, Maia
AU  - Nadaraia, Nanuli
AU  - Barbakadze, Nana
AU  - Amiranashvili, Lela
AU  - Kakhabrishvili, M
AU  - Petrou, Anthi
AU  - Carević, Tamara
AU  - Glamočlija, Jasmina
AU  - Geronikaki, Athina
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6556
AB  - Most of pharmaceutical agents display several or even many biological activities. It is obvious that testing even one compound for thousands of biological activities is a practically not reasonable task. Therefore, computer-aided prediction is the method of choice for the selection of the most promising bioassays for particular compounds. Using PASS Online software, we determined the probable antimicrobial activity of the 31 steroid derivatives. Experimental testing of the antimicrobial activity of the tested compounds by microdilution method confirmed the computational predictions. Furthermore, P. aeruginosa and C. albicans biofilm formation was investigated. Compound 11 showed a biofilm reduction by 42.26% at the MIC of the tested compound. The percentages are lower than ketoconazole, but very close to its activity.
PB  - Abingdon: Taylor and Francis
T2  - SAR and QSAR in Environmental Research
T1  - Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies
IS  - 2
VL  - 35
DO  - 10.1080/1062936X.2024.2309183
SP  - 137
EP  - 155
ER  - 

@article{
author = "Marlani, Maia and Nadaraia, Nanuli and Barbakadze, Nana and Amiranashvili, Lela and Kakhabrishvili, M and Petrou, Anthi and Carević, Tamara and Glamočlija, Jasmina and Geronikaki, Athina",
year = "2024",
abstract = "Most of pharmaceutical agents display several or even many biological activities. It is obvious that testing even one compound for thousands of biological activities is a practically not reasonable task. Therefore, computer-aided prediction is the method of choice for the selection of the most promising bioassays for particular compounds. Using PASS Online software, we determined the probable antimicrobial activity of the 31 steroid derivatives. Experimental testing of the antimicrobial activity of the tested compounds by microdilution method confirmed the computational predictions. Furthermore, P. aeruginosa and C. albicans biofilm formation was investigated. Compound 11 showed a biofilm reduction by 42.26% at the MIC of the tested compound. The percentages are lower than ketoconazole, but very close to its activity.",
publisher = "Abingdon: Taylor and Francis",
journal = "SAR and QSAR in Environmental Research",
title = "Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies",
number = "2",
volume = "35",
doi = "10.1080/1062936X.2024.2309183",
pages = "137-155"
}

Marlani, M., Nadaraia, N., Barbakadze, N., Amiranashvili, L., Kakhabrishvili, M., Petrou, A., Carević, T., Glamočlija, J.,& Geronikaki, A.. (2024). Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies. in SAR and QSAR in Environmental Research
Abingdon: Taylor and Francis., 35(2), 137-155.
https://doi.org/10.1080/1062936X.2024.2309183

Marlani M, Nadaraia N, Barbakadze N, Amiranashvili L, Kakhabrishvili M, Petrou A, Carević T, Glamočlija J, Geronikaki A. Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies. in SAR and QSAR in Environmental Research. 2024;35(2):137-155.
doi:10.1080/1062936X.2024.2309183 .

Marlani, Maia, Nadaraia, Nanuli, Barbakadze, Nana, Amiranashvili, Lela, Kakhabrishvili, M, Petrou, Anthi, Carević, Tamara, Glamočlija, Jasmina, Geronikaki, Athina, "Steroidal hydrazones as antimicrobial agents: biological evaluation and molecular docking studies" in SAR and QSAR in Environmental Research, 35, no. 2 (2024):137-155,
https://doi.org/10.1080/1062936X.2024.2309183 . .

TY  - JOUR
AU  - Batinić, Petar
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Carević, Tamara
AU  - Prijić, Željana
AU  - Marinković, Aleksandar
AU  - Marković, Tatjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6680
AB  - This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.
PB  - Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"
T2  - Lekovite sirovine
T1  - Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia
IS  - 1
VL  - 43
DO  - 10.61652/leksir2343e159B
SP  - e159
ER  - 

@article{
author = "Batinić, Petar and Čutović, Natalija and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Carević, Tamara and Prijić, Željana and Marinković, Aleksandar and Marković, Tatjana",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.",
publisher = "Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"",
journal = "Lekovite sirovine",
title = "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia",
number = "1",
volume = "43",
doi = "10.61652/leksir2343e159B",
pages = "e159"
}

Batinić, P., Čutović, N., Jovanović, A., Stojković, D., Zengin, G., Carević, T., Prijić, Ž., Marinković, A.,& Marković, T.. (2023). Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine
Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"., 43(1), e159.
https://doi.org/10.61652/leksir2343e159B

Batinić P, Čutović N, Jovanović A, Stojković D, Zengin G, Carević T, Prijić Ž, Marinković A, Marković T. Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine. 2023;43(1):e159.
doi:10.61652/leksir2343e159B .

Batinić, Petar, Čutović, Natalija, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Carević, Tamara, Prijić, Željana, Marinković, Aleksandar, Marković, Tatjana, "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia" in Lekovite sirovine, 43, no. 1 (2023):e159,
https://doi.org/10.61652/leksir2343e159B . .

TY  - JOUR
AU  - Merlani, Maia
AU  - Nadaraia, Nanuli
AU  - Amiranashvili, Lela
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Carević, Tamara
AU  - Soković, Marina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5659
AB  - Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.
PB  - Basel: MDPI
T2  - Molecules
T1  - Antimicrobial Activity of Some Steroidal Hydrazones
IS  - 3
VL  - 28
DO  - 10.3390/molecules28031167
SP  - 1167
ER  - 

@article{
author = "Merlani, Maia and Nadaraia, Nanuli and Amiranashvili, Lela and Petrou, Anthi and Geronikaki, Athina and Ćirić, Ana and Glamočlija, Jasmina and Carević, Tamara and Soković, Marina",
year = "2023",
abstract = "Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Antimicrobial Activity of Some Steroidal Hydrazones",
number = "3",
volume = "28",
doi = "10.3390/molecules28031167",
pages = "1167"
}

Merlani, M., Nadaraia, N., Amiranashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J., Carević, T.,& Soković, M.. (2023). Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules
Basel: MDPI., 28(3), 1167.
https://doi.org/10.3390/molecules28031167

Merlani M, Nadaraia N, Amiranashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Carević T, Soković M. Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules. 2023;28(3):1167.
doi:10.3390/molecules28031167 .

Merlani, Maia, Nadaraia, Nanuli, Amiranashvili, Lela, Petrou, Anthi, Geronikaki, Athina, Ćirić, Ana, Glamočlija, Jasmina, Carević, Tamara, Soković, Marina, "Antimicrobial Activity of Some Steroidal Hydrazones" in Molecules, 28, no. 3 (2023):1167,
https://doi.org/10.3390/molecules28031167 . .

TY  - CONF
AU  - Carević, Tamara
AU  - Novović, Katarina
AU  - Ivanov, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6439
AB  - Introduction: Different sugars are commonly used in the diet, but little is understood about the various effects of human health that they can affect. Hence, the impact of sugars and their substitutes used in diet on the development of virulence in Pseudomonas aeruginosa PAO1 was investigated. Sugars (fructose, demerara, coconut sugar, and cane sugar) and sugar substitutes (erythritol and stevia) were selected. The genes from three P. aeruginosa QS networks (las - lasI, lasR; rhl - rhlI, rhlR; PQS - pqsA, mvfR) were used for RT-qPCR analysis in order to investigate whether the expression of these genes changes. In this work, the focus is on the expression of genes involved in QS and the ability to form biofilms (a type of structured community of microorganisms that is attached to the surface and connected by an exopolysaccharide matrix), as well as determining minimal inhibitory concentration of antibiotics in presence of tested compounds.
Methods: Microdiltuion assay, Antibiofilm assay, RT- qPCR
Results: In the presence of tested sugars and their substitutes, the minimum inhibitory concentration of commercial antibiotics increased, as well as the percentages of biofilm formation (for instance, the percentage of biofilm formation is 171% in the presence of coconut sugar). Furthermore, exposure of P. aeruginosa to tested compounds caused the greatest increase in expression of virulence associated with the lasI and pvdF genes.
Conclusion: More awareness and research is needed to highlight the effects sugars can have on P. aeruginosa and to propose new strategies to reduce this negative aspect.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6439
ER  - 

@conference{
author = "Carević, Tamara and Novović, Katarina and Ivanov, Marija",
year = "2023",
abstract = "Introduction: Different sugars are commonly used in the diet, but little is understood about the various effects of human health that they can affect. Hence, the impact of sugars and their substitutes used in diet on the development of virulence in Pseudomonas aeruginosa PAO1 was investigated. Sugars (fructose, demerara, coconut sugar, and cane sugar) and sugar substitutes (erythritol and stevia) were selected. The genes from three P. aeruginosa QS networks (las - lasI, lasR; rhl - rhlI, rhlR; PQS - pqsA, mvfR) were used for RT-qPCR analysis in order to investigate whether the expression of these genes changes. In this work, the focus is on the expression of genes involved in QS and the ability to form biofilms (a type of structured community of microorganisms that is attached to the surface and connected by an exopolysaccharide matrix), as well as determining minimal inhibitory concentration of antibiotics in presence of tested compounds.
Methods: Microdiltuion assay, Antibiofilm assay, RT- qPCR
Results: In the presence of tested sugars and their substitutes, the minimum inhibitory concentration of commercial antibiotics increased, as well as the percentages of biofilm formation (for instance, the percentage of biofilm formation is 171% in the presence of coconut sugar). Furthermore, exposure of P. aeruginosa to tested compounds caused the greatest increase in expression of virulence associated with the lasI and pvdF genes.
Conclusion: More awareness and research is needed to highlight the effects sugars can have on P. aeruginosa and to propose new strategies to reduce this negative aspect.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6439"
}

Carević, T., Novović, K.,& Ivanov, M.. (2023). Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 48.
https://hdl.handle.net/21.15107/rcub_ibiss_6439

Carević T, Novović K, Ivanov M. Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:48.
https://hdl.handle.net/21.15107/rcub_ibiss_6439 .

Carević, Tamara, Novović, Katarina, Ivanov, Marija, "Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):48,
https://hdl.handle.net/21.15107/rcub_ibiss_6439 .

TY  - CONF
AU  - Carević, Tamara
AU  - Petrović, Jovana
AU  - Ivanov, Marija
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
AU  - Ćirić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6391
AB  - Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima.
AB  - The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.
PB  - Novi Sad: Institute of Field and Vegetable Crops
C3  - Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
T1  - Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima
T1  - Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages
SP  - 24
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6391
ER  - 

@conference{
author = "Carević, Tamara and Petrović, Jovana and Ivanov, Marija and Milinčić, Danijel and Pešić, Mirjana and Ćirić, Ana",
year = "2023",
abstract = "Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima., The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.",
publisher = "Novi Sad: Institute of Field and Vegetable Crops",
journal = "Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia",
title = "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima, Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages",
pages = "24-27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6391"
}

Carević, T., Petrović, J., Ivanov, M., Milinčić, D., Pešić, M.,& Ćirić, A.. (2023). Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
Novi Sad: Institute of Field and Vegetable Crops., 24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391

Carević T, Petrović J, Ivanov M, Milinčić D, Pešić M, Ćirić A. Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia. 2023;:24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

Carević, Tamara, Petrović, Jovana, Ivanov, Marija, Milinčić, Danijel, Pešić, Mirjana, Ćirić, Ana, "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima" in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia (2023):24-27,
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

TY  - JOUR
AU  - Carević, Tamara
AU  - Stojković, Dejan
AU  - Ivanov, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6073
AB  - Flavonoids are secondary metabolites abundantly present in plants and, in most cases, essential contributors to plants bioactivity. They have been studied so far for a range of possible health-beneficial effects, including antioxidant, cardioprotective, and cytotoxic. Therefore, there are data on the antimicrobial potential of a significant number of flavonoids. However, less is known regarding their antivirulence traits. Trending antimicrobial research worldwide has pointed out the promising effects of antimicrobial strategies based on the antivirulence principle, so this review aims to present the newest research regarding the antivirulence effects of flavonoids. Articles on antivirulence flavonoids published from 2015 until now were selected. A range of molecules from this class has been studied up to date, with the most abundant data for quercetin and myricetin, while the most studied organism is Pseudomonas aeruginosa</i>. The antivirulence attributes studied included antibiofilm assessment, followed by data on the inhibition of virulence pigments (pyocyanin, violacein, and staphyloxanthin) and virulence enzyme production (such as sortase A and elastase). Less information is collected on the inhibition of morphological transition, motility, and molecular mechanisms underlying the antivirulence properties of flavonoids and in vivo</i> research. Flavonoids are a group of compounds with a wide range of antivirulence traits and might be further developed into essential parts of novel antimicrobial strategies.
PB  - Bentham Science Publication Ltd.
T2  - Current Pharmaceutical Design
T1  - Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update
IS  - 12
VL  - 29
DO  - 10.2174/1381612829666230413085029
SP  - 914
EP  - 927
ER  - 

@article{
author = "Carević, Tamara and Stojković, Dejan and Ivanov, Marija",
year = "2023",
abstract = "Flavonoids are secondary metabolites abundantly present in plants and, in most cases, essential contributors to plants bioactivity. They have been studied so far for a range of possible health-beneficial effects, including antioxidant, cardioprotective, and cytotoxic. Therefore, there are data on the antimicrobial potential of a significant number of flavonoids. However, less is known regarding their antivirulence traits. Trending antimicrobial research worldwide has pointed out the promising effects of antimicrobial strategies based on the antivirulence principle, so this review aims to present the newest research regarding the antivirulence effects of flavonoids. Articles on antivirulence flavonoids published from 2015 until now were selected. A range of molecules from this class has been studied up to date, with the most abundant data for quercetin and myricetin, while the most studied organism is Pseudomonas aeruginosa</i>. The antivirulence attributes studied included antibiofilm assessment, followed by data on the inhibition of virulence pigments (pyocyanin, violacein, and staphyloxanthin) and virulence enzyme production (such as sortase A and elastase). Less information is collected on the inhibition of morphological transition, motility, and molecular mechanisms underlying the antivirulence properties of flavonoids and in vivo</i> research. Flavonoids are a group of compounds with a wide range of antivirulence traits and might be further developed into essential parts of novel antimicrobial strategies.",
publisher = "Bentham Science Publication Ltd.",
journal = "Current Pharmaceutical Design",
title = "Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update",
number = "12",
volume = "29",
doi = "10.2174/1381612829666230413085029",
pages = "914-927"
}

Carević, T., Stojković, D.,& Ivanov, M.. (2023). Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update. in Current Pharmaceutical Design
Bentham Science Publication Ltd.., 29(12), 914-927.
https://doi.org/10.2174/1381612829666230413085029

Carević T, Stojković D, Ivanov M. Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update. in Current Pharmaceutical Design. 2023;29(12):914-927.
doi:10.2174/1381612829666230413085029 .

Carević, Tamara, Stojković, Dejan, Ivanov, Marija, "Plant Flavonoids as Reservoirs of Therapeutics against Microbial Virulence Traits: A Comprehensive Review Update" in Current Pharmaceutical Design, 29, no. 12 (2023):914-927,
https://doi.org/10.2174/1381612829666230413085029 . .

TY  - JOUR
AU  - Marković, Tatjana
AU  - Čutović, Natalija
AU  - Carević, Tamara
AU  - Gašić, Uroš
AU  - Stojković, Dejan
AU  - Xue, Jingqi
AU  - Jovanović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6072
AB  - Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts
IS  - 14
VL  - 24
DO  - 10.3390/ijms241411764
SP  - 11764
ER  - 

@article{
author = "Marković, Tatjana and Čutović, Natalija and Carević, Tamara and Gašić, Uroš and Stojković, Dejan and Xue, Jingqi and Jovanović, Aleksandra",
year = "2023",
abstract = "Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts",
number = "14",
volume = "24",
doi = "10.3390/ijms241411764",
pages = "11764"
}

Marković, T., Čutović, N., Carević, T., Gašić, U., Stojković, D., Xue, J.,& Jovanović, A.. (2023). Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences
Basel: MDPI., 24(14), 11764.
https://doi.org/10.3390/ijms241411764

Marković T, Čutović N, Carević T, Gašić U, Stojković D, Xue J, Jovanović A. Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences. 2023;24(14):11764.
doi:10.3390/ijms241411764 .

Marković, Tatjana, Čutović, Natalija, Carević, Tamara, Gašić, Uroš, Stojković, Dejan, Xue, Jingqi, Jovanović, Aleksandra, "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts" in International Journal of Molecular Sciences, 24, no. 14 (2023):11764,
https://doi.org/10.3390/ijms241411764 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Petrović, Jovana
AU  - Carević, Tamara
AU  - Soković, Marina
AU  - Liaras, Konstantinos
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5793
AB  - This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review
IS  - 6
VL  - 12
DO  - 10.3390/antibiotics12060963
SP  - 963
ER  - 

@article{
author = "Stojković, Dejan and Petrović, Jovana and Carević, Tamara and Soković, Marina and Liaras, Konstantinos",
year = "2023",
abstract = "This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review",
number = "6",
volume = "12",
doi = "10.3390/antibiotics12060963",
pages = "963"
}

Stojković, D., Petrović, J., Carević, T., Soković, M.,& Liaras, K.. (2023). Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics
Basel: MDPI., 12(6), 963.
https://doi.org/10.3390/antibiotics12060963

Stojković D, Petrović J, Carević T, Soković M, Liaras K. Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics. 2023;12(6):963.
doi:10.3390/antibiotics12060963 .

Stojković, Dejan, Petrović, Jovana, Carević, Tamara, Soković, Marina, Liaras, Konstantinos, "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review" in Antibiotics, 12, no. 6 (2023):963,
https://doi.org/10.3390/antibiotics12060963 . .

TY  - JOUR
AU  - Babota, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Barros, Lillian
AU  - Añibarro-Ortega, Mikel
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Mocan, Andrei
AU  - López, Víctor
AU  - Crișan, Gianina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5475
AB  - Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020390
SP  - 390
ER  - 

@article{
author = "Babota, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Dias, Maria Inês and Pinela, José and Barros, Lillian and Añibarro-Ortega, Mikel and Stojković, Dejan and Carević, Tamara and Mocan, Andrei and López, Víctor and Crișan, Gianina",
year = "2023",
abstract = "Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits",
number = "2",
volume = "12",
doi = "10.3390/antiox12020390",
pages = "390"
}

Babota, M., Frumuzachi, O., Nicolescu, A., Dias, M. I., Pinela, J., Barros, L., Añibarro-Ortega, M., Stojković, D., Carević, T., Mocan, A., López, V.,& Crișan, G.. (2023). Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants
Basel: MDPI., 12(2), 390.
https://doi.org/10.3390/antiox12020390

Babota M, Frumuzachi O, Nicolescu A, Dias MI, Pinela J, Barros L, Añibarro-Ortega M, Stojković D, Carević T, Mocan A, López V, Crișan G. Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants. 2023;12(2):390.
doi:10.3390/antiox12020390 .

Babota, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Dias, Maria Inês, Pinela, José, Barros, Lillian, Añibarro-Ortega, Mikel, Stojković, Dejan, Carević, Tamara, Mocan, Andrei, López, Víctor, Crișan, Gianina, "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits" in Antioxidants, 12, no. 2 (2023):390,
https://doi.org/10.3390/antiox12020390 . .

TY  - CONF
AU  - Carević, Tamara
AU  - Glamočlija, Jasmina
AU  - Nikolić, Biljana
AU  - Ivanov, Marija
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5332
AB  - The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.
PB  - Novi Sad: Matica srpska
C3  - Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
T1  - Mechanisms of action of selected flavonoids towards species of the genus Candida
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5332
ER  - 

@conference{
author = "Carević, Tamara and Glamočlija, Jasmina and Nikolić, Biljana and Ivanov, Marija and Soković, Marina",
year = "2022",
abstract = "The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.",
publisher = "Novi Sad: Matica srpska",
journal = "Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia",
title = "Mechanisms of action of selected flavonoids towards species of the genus Candida",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5332"
}

Carević, T., Glamočlija, J., Nikolić, B., Ivanov, M.,& Soković, M.. (2022). Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
Novi Sad: Matica srpska., 46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332

Carević T, Glamočlija J, Nikolić B, Ivanov M, Soković M. Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia. 2022;:46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .

Carević, Tamara, Glamočlija, Jasmina, Nikolić, Biljana, Ivanov, Marija, Soković, Marina, "Mechanisms of action of selected flavonoids towards species of the genus Candida" in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia (2022):46,
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .

TY  - JOUR
AU  - Carević, Tamara
AU  - Kostić, Marina
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ivanov, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5146
AB  - Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations
PB  - Basel: MDPI
T2  - Molecules
T1  - Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity
IS  - 20
VL  - 27
DO  - 10.3390/molecules27206806
SP  - 6806
ER  - 

@article{
author = "Carević, Tamara and Kostić, Marina and Nikolić, Biljana and Stojković, Dejan and Soković, Marina and Ivanov, Marija",
year = "2022",
abstract = "Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity",
number = "20",
volume = "27",
doi = "10.3390/molecules27206806",
pages = "6806"
}

Carević, T., Kostić, M., Nikolić, B., Stojković, D., Soković, M.,& Ivanov, M.. (2022). Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules
Basel: MDPI., 27(20), 6806.
https://doi.org/10.3390/molecules27206806

Carević T, Kostić M, Nikolić B, Stojković D, Soković M, Ivanov M. Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules. 2022;27(20):6806.
doi:10.3390/molecules27206806 .

Carević, Tamara, Kostić, Marina, Nikolić, Biljana, Stojković, Dejan, Soković, Marina, Ivanov, Marija, "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity" in Molecules, 27, no. 20 (2022):6806,
https://doi.org/10.3390/molecules27206806 . .

TY  - CONF
AU  - Ivanov, Marija
AU  - Carević, Tamara
AU  - Kostić, Marina
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5751
AB  - Terpenoids are the most complex class of natural products with over 80000 identified representatives found in plants, microorganisms, insects, and marine invertebrates. Their anticandidal potential, especially impact on fungal virulence is, however, not fully explored yet. We aimed to investigate anticandidal, antibiofilm, and antihyphal properties of selected terpenoids (borneol, thymol, geraniol, and carvacrol). The strongest antifungal potential towards Candida albicans strains could be noticed for borneol (lowest minimal inhibitory concentration, MIC, 0.06 mg/mL) followed by thymol (lowest MIC 0.12 mg/mL). On the other hand, MIC of geraniol was up to 8 mg/mL. Non-albicans Candida strains were most susceptible to thymol (MIC 0.12 mg/mL towards C. krusei H1/16) and borneol (MIC 0.12 mg/mL towards C. parapsilosis ATCC 22019). When the terpenoids were applied in their previously determined minimal inhibitory concentrations the strongest reducing impact on C. albicans ATCC 10231 biofilm forming ability was observed for carvacrol (60% inhibition), followed by geraniol (56% inhibition). Lower concentration of terpenoids (0.5 MIC) led to similar impairment of biofilm formation – the strongest impact was noticed for geraniol (54% inhibition), followed by carvacrol (47% inhibition) as determined by crystal violet assay. MTT antibiofilm assay highlighted great potential of carvacrol and geraniol to reduce viability of Candida cells in biofilms. Inhibition of hyphal formation could be noticed only upon application of carvacrol (14.29% of hyphal cells compared to the untreated control with 50.67% hyphae) suggesting wide antivirulence potential exhibited by this molecule. Other compounds explored in this study have displayed high MIC values (geraniol), low antibiofilm potential (thymol, borneol) or lack of antihyphal properties (all except carvacrol). Potential of carvacrol to reduce fungal virulence should be further studied on molecular level and in in vivo assays.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia
T1  - Selected terpenoids as obstructers of Candida virulence
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5751
ER  - 

@conference{
author = "Ivanov, Marija and Carević, Tamara and Kostić, Marina and Ćirić, Ana and Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Terpenoids are the most complex class of natural products with over 80000 identified representatives found in plants, microorganisms, insects, and marine invertebrates. Their anticandidal potential, especially impact on fungal virulence is, however, not fully explored yet. We aimed to investigate anticandidal, antibiofilm, and antihyphal properties of selected terpenoids (borneol, thymol, geraniol, and carvacrol). The strongest antifungal potential towards Candida albicans strains could be noticed for borneol (lowest minimal inhibitory concentration, MIC, 0.06 mg/mL) followed by thymol (lowest MIC 0.12 mg/mL). On the other hand, MIC of geraniol was up to 8 mg/mL. Non-albicans Candida strains were most susceptible to thymol (MIC 0.12 mg/mL towards C. krusei H1/16) and borneol (MIC 0.12 mg/mL towards C. parapsilosis ATCC 22019). When the terpenoids were applied in their previously determined minimal inhibitory concentrations the strongest reducing impact on C. albicans ATCC 10231 biofilm forming ability was observed for carvacrol (60% inhibition), followed by geraniol (56% inhibition). Lower concentration of terpenoids (0.5 MIC) led to similar impairment of biofilm formation – the strongest impact was noticed for geraniol (54% inhibition), followed by carvacrol (47% inhibition) as determined by crystal violet assay. MTT antibiofilm assay highlighted great potential of carvacrol and geraniol to reduce viability of Candida cells in biofilms. Inhibition of hyphal formation could be noticed only upon application of carvacrol (14.29% of hyphal cells compared to the untreated control with 50.67% hyphae) suggesting wide antivirulence potential exhibited by this molecule. Other compounds explored in this study have displayed high MIC values (geraniol), low antibiofilm potential (thymol, borneol) or lack of antihyphal properties (all except carvacrol). Potential of carvacrol to reduce fungal virulence should be further studied on molecular level and in in vivo assays.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia",
title = "Selected terpenoids as obstructers of Candida virulence",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5751"
}

Ivanov, M., Carević, T., Kostić, M., Ćirić, A., Petrović, J., Glamočlija, J.,& Soković, M.. (2022). Selected terpenoids as obstructers of Candida virulence. in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture..
https://hdl.handle.net/21.15107/rcub_ibiss_5751

Ivanov M, Carević T, Kostić M, Ćirić A, Petrović J, Glamočlija J, Soković M. Selected terpenoids as obstructers of Candida virulence. in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5751 .

Ivanov, Marija, Carević, Tamara, Kostić, Marina, Ćirić, Ana, Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, "Selected terpenoids as obstructers of Candida virulence" in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5751 .

TY  - CONF
AU  - Carević, Tamara
AU  - Ivanov, Marija
AU  - Kostić, Marina
AU  - Nikolić, Biljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5334
AB  - Услед све веће учесталости антимикробне резистенције, постоји потреба за
проналаском нових антифунгалних агенаса. Проблем у лечењу инфекција
изазваних врстама рода Candida је високо отпоран биофилм чија отпорност је
последица присуства матрикса богатог егзополисахардима. Испитан је
антифунгални спектар хесперетина, флавоноида присутног у цитрусном воћу,
према различитим сојевима Candida, са посебним освртом на фунгални биофилм,
као и његова токсичност на моделу Artemia salinа и ћелија фибробласта плућа.
Минимална инхибиторна концентрација хесперетина, износи 0,165 mg/mL према
свих осам тестираних сојева. Утицај на инхибицију формирања биофилма је
најизраженији према C. glabrata 4/6/15 (70,4% инхибиције), док је 24 h стар
биофилм C. albicans ATCC 10231 био најподложнији третману хесперетином
(74,41% деструкције). Поред утицаја на спречавање формирања/деструкцију
биофилма хесперетин се показао ефикасним антибиофилм агенсом и способношћу
да умањи продукцију егзополисахарида у матриксу фунгалног биофилма. У тесту
цитотоксичности на испитиваној ћелијској линији, хесперетин је показао
селективну токсичност (IC50 0,340 mg/mL), док се на моделу Artemia salinа
хесперетин показао као нетоксичан, LC50 > 1 mg/mL. Узевши у обзир широк
спектар антифунгалне активности хесперетина и његову нетоксичност, овај
флавоноид поседује потенцијал да се даљим истраживањем додатно испита као
нови антифунгални агенс
AB  - Usled sve veće učestalosti antimikrobne rezistencije, postoji potreba za pronalaskom novih antifungalnih agenasa. Problem u lečenju infekcija izazvanih vrstama roda Candida je visoko otporan biofilm čija otpornost je posledica prisustva matriksa bogatog egzopolisahardima. Ispitan je antifungalni spektar hesperetina, flavonoida prisutnog u citrusnom voću, prema različitim sojevima Candida, sa posebnim osvrtom na fungalni biofilm, kao i njegova toksičnost na modelu Artemia salina i ćelija fibroblasta pluća. Minimalna inhibitorna koncentracija hesperetina, iznosi 0,165 mg/mL prema svih osam testiranih sojeva. Uticaj na inhibiciju formiranja biofilma je najizraženiji prema C. glabrata 4/6/15 (70,4% inhibicije), dok je 24 h star biofilm C. albicans ATCC 10231 bio najpodložniji tretmanu hesperetinom (74,41% destrukcije). Pored uticaja na sprečavanje formiranja/destrukciju biofilma hesperetin se pokazao efikasnim antibiofilm agensom i sposobnošću da umanji produkciju egzopolisaharida u matriksu fungalnog biofilma. U testu citotoksičnosti na ispitivanoj ćelijskoj liniji, hesperetin je pokazao selektivnu toksičnost (IC50 0,340 mg/mL), dok se na modelu Artemia salina hesperetin pokazao kao netoksičan, LC50 > 1 mg/mL. Uzevši u obzir širok spektar antifungalne aktivnosti hesperetina i njegovu netoksičnost, ovaj flavonoid poseduje potencijal da se daljim istraživanjem dodatno ispita kao novi antifungalni agens
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Antifungalni potencijal i toksičnost hesperetina
T1  - Антифунгални потенцијал и токсичност хесперетина
SP  - 242
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5334
ER  - 

@conference{
author = "Carević, Tamara and Ivanov, Marija and Kostić, Marina and Nikolić, Biljana",
year = "2022",
abstract = "Услед све веће учесталости антимикробне резистенције, постоји потреба за
проналаском нових антифунгалних агенаса. Проблем у лечењу инфекција
изазваних врстама рода Candida је високо отпоран биофилм чија отпорност је
последица присуства матрикса богатог егзополисахардима. Испитан је
антифунгални спектар хесперетина, флавоноида присутног у цитрусном воћу,
према различитим сојевима Candida, са посебним освртом на фунгални биофилм,
као и његова токсичност на моделу Artemia salinа и ћелија фибробласта плућа.
Минимална инхибиторна концентрација хесперетина, износи 0,165 mg/mL према
свих осам тестираних сојева. Утицај на инхибицију формирања биофилма је
најизраженији према C. glabrata 4/6/15 (70,4% инхибиције), док је 24 h стар
биофилм C. albicans ATCC 10231 био најподложнији третману хесперетином
(74,41% деструкције). Поред утицаја на спречавање формирања/деструкцију
биофилма хесперетин се показао ефикасним антибиофилм агенсом и способношћу
да умањи продукцију егзополисахарида у матриксу фунгалног биофилма. У тесту
цитотоксичности на испитиваној ћелијској линији, хесперетин је показао
селективну токсичност (IC50 0,340 mg/mL), док се на моделу Artemia salinа
хесперетин показао као нетоксичан, LC50 > 1 mg/mL. Узевши у обзир широк
спектар антифунгалне активности хесперетина и његову нетоксичност, овај
флавоноид поседује потенцијал да се даљим истраживањем додатно испита као
нови антифунгални агенс, Usled sve veće učestalosti antimikrobne rezistencije, postoji potreba za pronalaskom novih antifungalnih agenasa. Problem u lečenju infekcija izazvanih vrstama roda Candida je visoko otporan biofilm čija otpornost je posledica prisustva matriksa bogatog egzopolisahardima. Ispitan je antifungalni spektar hesperetina, flavonoida prisutnog u citrusnom voću, prema različitim sojevima Candida, sa posebnim osvrtom na fungalni biofilm, kao i njegova toksičnost na modelu Artemia salina i ćelija fibroblasta pluća. Minimalna inhibitorna koncentracija hesperetina, iznosi 0,165 mg/mL prema svih osam testiranih sojeva. Uticaj na inhibiciju formiranja biofilma je najizraženiji prema C. glabrata 4/6/15 (70,4% inhibicije), dok je 24 h star biofilm C. albicans ATCC 10231 bio najpodložniji tretmanu hesperetinom (74,41% destrukcije). Pored uticaja na sprečavanje formiranja/destrukciju biofilma hesperetin se pokazao efikasnim antibiofilm agensom i sposobnošću da umanji produkciju egzopolisaharida u matriksu fungalnog biofilma. U testu citotoksičnosti na ispitivanoj ćelijskoj liniji, hesperetin je pokazao selektivnu toksičnost (IC50 0,340 mg/mL), dok se na modelu Artemia salina hesperetin pokazao kao netoksičan, LC50 > 1 mg/mL. Uzevši u obzir širok spektar antifungalne aktivnosti hesperetina i njegovu netoksičnost, ovaj flavonoid poseduje potencijal da se daljim istraživanjem dodatno ispita kao novi antifungalni agens",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Antifungalni potencijal i toksičnost hesperetina, Антифунгални потенцијал и токсичност хесперетина",
pages = "242",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5334"
}

Carević, T., Ivanov, M., Kostić, M.,& Nikolić, B.. (2022). Antifungalni potencijal i toksičnost hesperetina. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 242.
https://hdl.handle.net/21.15107/rcub_ibiss_5334

Carević T, Ivanov M, Kostić M, Nikolić B. Antifungalni potencijal i toksičnost hesperetina. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:242.
https://hdl.handle.net/21.15107/rcub_ibiss_5334 .

Carević, Tamara, Ivanov, Marija, Kostić, Marina, Nikolić, Biljana, "Antifungalni potencijal i toksičnost hesperetina" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):242,
https://hdl.handle.net/21.15107/rcub_ibiss_5334 .

TY  - CONF
AU  - Carević, Tamara
AU  - Novović, Katarina
AU  - Ivanov, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5333
AB  - Different sweeteners are used in diet but not much is known about different aspects of human
health that they can affect. Consequently, the influence of sweeteners used in nutrition on the development of resistance and virulence of microorganisms (species of the genus Candida, Pseudomonas, Staphylococcus) were examined. Three plant-derived sweeteners were selected: demerara
sugar (from sugar cane), stevia (isolated from Stevia rebaudiana), and coconut sugar (from coconut
palm). An increase in the minimum inhibitory concentration of commercial antibiotics in the presence of studied sweeteners has been observed. Also, an increase in the biofilm production ability
in the presence of sweeteners is noticed for all the species except C. albicans: demerara sugar (P.
aeruginosa 528%, S. aureus 262%, C. albicans 64%), stevia (P. aeruginosa 140%, S. aureus 180%, C.
albicans 79%), coconut sugar (P. aeruginosa 408%, S. aureus 307%, C. albicans 57%) as compared
to the untreated control biofilm production (100%). Exposure of P. aeruginosa to demerara sugar
induced increase in expression of virulence associated lasI and lasR genes. On the other hand, in
C. albicans no significant increase in formation of hyphae in the presence of sweeteners has been
observed (demerara sugar induced hyphae to 113%, stevia 72%, coconut sugar 13%, compared to
the untreated control). Plant derived sweeteners increase virulence of bacterial species, but not C.
albicans. Higher awareness and further research is needed in order to highlight the effects plant
derived sweeteners could induce in pathogenic bacteria and to provide novel tools to reduce this
negative aspect.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Plant derived sweeteners promote virulence of pathogenic bacteria
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5333
ER  - 

@conference{
author = "Carević, Tamara and Novović, Katarina and Ivanov, Marija",
year = "2022",
abstract = "Different sweeteners are used in diet but not much is known about different aspects of human
health that they can affect. Consequently, the influence of sweeteners used in nutrition on the development of resistance and virulence of microorganisms (species of the genus Candida, Pseudomonas, Staphylococcus) were examined. Three plant-derived sweeteners were selected: demerara
sugar (from sugar cane), stevia (isolated from Stevia rebaudiana), and coconut sugar (from coconut
palm). An increase in the minimum inhibitory concentration of commercial antibiotics in the presence of studied sweeteners has been observed. Also, an increase in the biofilm production ability
in the presence of sweeteners is noticed for all the species except C. albicans: demerara sugar (P.
aeruginosa 528%, S. aureus 262%, C. albicans 64%), stevia (P. aeruginosa 140%, S. aureus 180%, C.
albicans 79%), coconut sugar (P. aeruginosa 408%, S. aureus 307%, C. albicans 57%) as compared
to the untreated control biofilm production (100%). Exposure of P. aeruginosa to demerara sugar
induced increase in expression of virulence associated lasI and lasR genes. On the other hand, in
C. albicans no significant increase in formation of hyphae in the presence of sweeteners has been
observed (demerara sugar induced hyphae to 113%, stevia 72%, coconut sugar 13%, compared to
the untreated control). Plant derived sweeteners increase virulence of bacterial species, but not C.
albicans. Higher awareness and further research is needed in order to highlight the effects plant
derived sweeteners could induce in pathogenic bacteria and to provide novel tools to reduce this
negative aspect.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Plant derived sweeteners promote virulence of pathogenic bacteria",
pages = "116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5333"
}

Carević, T., Novović, K.,& Ivanov, M.. (2022). Plant derived sweeteners promote virulence of pathogenic bacteria. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 116.
https://hdl.handle.net/21.15107/rcub_ibiss_5333

Carević T, Novović K, Ivanov M. Plant derived sweeteners promote virulence of pathogenic bacteria. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:116.
https://hdl.handle.net/21.15107/rcub_ibiss_5333 .

Carević, Tamara, Novović, Katarina, Ivanov, Marija, "Plant derived sweeteners promote virulence of pathogenic bacteria" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):116,
https://hdl.handle.net/21.15107/rcub_ibiss_5333 .