TY  - JOUR
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
AU  - Jerinić-Prodanović, Dušanka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6665
AB  - Abstract
Background Carrot is an important vegetable crop grown worldwide. The major economic problem in carrot cultivation is yellow disease caused by Bactericera trigonica, which induces biotic stress and has the greatest impact on crop productivity. Comprehensive studies on the mechanism of carrot defense response to biotic stress caused by B. trigonica infestation have yet to be conducted.
Methods The changes in photosynthetic pigments, proline, TPC, H2O2 and MDA content, DPPH radical scavenging ability, and antioxidant enzyme activity of SOD, CAT, and POX in carrot leaves in response to insect sex (female and male), rapid response (during the frst six hours), and long-term response to B. trigonica infestation were evaluated.
Results The results of our study strongly suggest that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and oxidative status of carrot leaves. Photosynthetic pigment content, TPC, and DPPH and CAT activities were signifcantly reduced in carrot leaves in response to insect infestation. On the other hand, proline, H2O2 content, and the activity of the antioxidant enzymes superoxide dismutase and peroxidase were increased in carrot leaves after B. trigonica infestation. The results indicate that B. trigonica attenuates and delays the oxidative stress responses of carrot, allowing long-term feeding without visible changes in the plant. Carrot responded to longterm B. trigonica infestation with an increase in SOD and POX activity, suggesting that these enzymes may play a key
role in plant defense mechanisms.
Conclusions This is the frst comprehensive study strongly suggesting that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and an attenuated ROS defense response in carrot leaves that enables long-term insect feeding. The information provides new insights into the mechanisms of carrot protection against B. trigonica infestation.
PB  - Springer Nature
T2  - BMC Plant Biology
T1  - Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation
IS  - 1
VL  - 24
DO  - 10.1186/s12870-024-04946-4
SP  - 243
ER  - 

@article{
author = "Đurić, Marija and Jevremović, Slađana and Trifunović-Momčilov, Milana and Milošević, Snežana and Subotić, Angelina and Jerinić-Prodanović, Dušanka",
year = "2024",
abstract = "Abstract
Background Carrot is an important vegetable crop grown worldwide. The major economic problem in carrot cultivation is yellow disease caused by Bactericera trigonica, which induces biotic stress and has the greatest impact on crop productivity. Comprehensive studies on the mechanism of carrot defense response to biotic stress caused by B. trigonica infestation have yet to be conducted.
Methods The changes in photosynthetic pigments, proline, TPC, H2O2 and MDA content, DPPH radical scavenging ability, and antioxidant enzyme activity of SOD, CAT, and POX in carrot leaves in response to insect sex (female and male), rapid response (during the frst six hours), and long-term response to B. trigonica infestation were evaluated.
Results The results of our study strongly suggest that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and oxidative status of carrot leaves. Photosynthetic pigment content, TPC, and DPPH and CAT activities were signifcantly reduced in carrot leaves in response to insect infestation. On the other hand, proline, H2O2 content, and the activity of the antioxidant enzymes superoxide dismutase and peroxidase were increased in carrot leaves after B. trigonica infestation. The results indicate that B. trigonica attenuates and delays the oxidative stress responses of carrot, allowing long-term feeding without visible changes in the plant. Carrot responded to longterm B. trigonica infestation with an increase in SOD and POX activity, suggesting that these enzymes may play a key
role in plant defense mechanisms.
Conclusions This is the frst comprehensive study strongly suggesting that B. trigonica infestation causes signifcant changes in primary and secondary metabolism and an attenuated ROS defense response in carrot leaves that enables long-term insect feeding. The information provides new insights into the mechanisms of carrot protection against B. trigonica infestation.",
publisher = "Springer Nature",
journal = "BMC Plant Biology",
title = "Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation",
number = "1",
volume = "24",
doi = "10.1186/s12870-024-04946-4",
pages = "243"
}

Đurić, M., Jevremović, S., Trifunović-Momčilov, M., Milošević, S., Subotić, A.,& Jerinić-Prodanović, D.. (2024). Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation. in BMC Plant Biology
Springer Nature., 24(1), 243.
https://doi.org/10.1186/s12870-024-04946-4

Đurić M, Jevremović S, Trifunović-Momčilov M, Milošević S, Subotić A, Jerinić-Prodanović D. Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation. in BMC Plant Biology. 2024;24(1):243.
doi:10.1186/s12870-024-04946-4 .

Đurić, Marija, Jevremović, Slađana, Trifunović-Momčilov, Milana, Milošević, Snežana, Subotić, Angelina, Jerinić-Prodanović, Dušanka, "Physiological and oxidative stress response of carrot (Daucus carota L.) to jumping plant-louse Bactericera trigonica Hodkinson (Hemiptera: Psylloidea) infestation" in BMC Plant Biology, 24, no. 1 (2024):243,
https://doi.org/10.1186/s12870-024-04946-4 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Jevremović, Slađana
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6522
AB  - The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010017
SP  - 17
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Jevremović, Slađana and Uzelac, Branka",
year = "2024",
abstract = "The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010017",
pages = "17"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Jevremović, S.,& Uzelac, B.. (2024). Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae
Basel : MDPI., 10(1), 17.
https://doi.org/10.3390/horticulturae10010017

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Jevremović S, Uzelac B. Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae. 2024;10(1):17.
doi:10.3390/horticulturae10010017 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Jevremović, Slađana, Uzelac, Branka, "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime" in Horticulturae, 10, no. 1 (2024):17,
https://doi.org/10.3390/horticulturae10010017 . .

TY  - JOUR
AU  - Popržen, Tatjana
AU  - Nikolić, Ivan
AU  - Krstić-Milošević, Dijana
AU  - Uzelac, Branka
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Radulović, Olga
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6442
AB  - The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)
IS  - 24
VL  - 24
DO  - 10.3390/ijms242417207
SP  - 17207
ER  - 

@article{
author = "Popržen, Tatjana and Nikolić, Ivan and Krstić-Milošević, Dijana and Uzelac, Branka and Trifunović-Momčilov, Milana and Marković, Marija and Radulović, Olga",
year = "2023",
abstract = "The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)",
number = "24",
volume = "24",
doi = "10.3390/ijms242417207",
pages = "17207"
}

Popržen, T., Nikolić, I., Krstić-Milošević, D., Uzelac, B., Trifunović-Momčilov, M., Marković, M.,& Radulović, O.. (2023). Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences
Basel : MDPI., 24(24), 17207.
https://doi.org/10.3390/ijms242417207

Popržen T, Nikolić I, Krstić-Milošević D, Uzelac B, Trifunović-Momčilov M, Marković M, Radulović O. Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences. 2023;24(24):17207.
doi:10.3390/ijms242417207 .

Popržen, Tatjana, Nikolić, Ivan, Krstić-Milošević, Dijana, Uzelac, Branka, Trifunović-Momčilov, Milana, Marković, Marija, Radulović, Olga, "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)" in International Journal of Molecular Sciences, 24, no. 24 (2023):17207,
https://doi.org/10.3390/ijms242417207 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Radulović, Olga
AU  - Subotić, Angelina
AU  - Uzelac, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6135
AB  - Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.
PB  - Basel: MDPI
T2  - Agriculture
T1  - Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?
IS  - 10
VL  - 13
DO  - 10.3390/agriculture13101903
SP  - 1903
ER  - 

@article{
author = "Đurić, Marija and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Radulović, Olga and Subotić, Angelina and Uzelac, Branka",
year = "2023",
abstract = "Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?",
number = "10",
volume = "13",
doi = "10.3390/agriculture13101903",
pages = "1903"
}

Đurić, M., Trifunović-Momčilov, M., Milošević, S., Marković, M., Radulović, O., Subotić, A.,& Uzelac, B.. (2023). Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture
Basel: MDPI., 13(10), 1903.
https://doi.org/10.3390/agriculture13101903

Đurić M, Trifunović-Momčilov M, Milošević S, Marković M, Radulović O, Subotić A, Uzelac B. Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture. 2023;13(10):1903.
doi:10.3390/agriculture13101903 .

Đurić, Marija, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Radulović, Olga, Subotić, Angelina, Uzelac, Branka, "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?" in Agriculture, 13, no. 10 (2023):1903,
https://doi.org/10.3390/agriculture13101903 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Uzelac, Branka
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6089
AB  - Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro
IS  - 8
VL  - 9
DO  - 10.3390/horticulturae9080910
SP  - 910
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Uzelac, Branka and Subotić, Angelina",
year = "2023",
abstract = "Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro",
number = "8",
volume = "9",
doi = "10.3390/horticulturae9080910",
pages = "910"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Uzelac, B.,& Subotić, A.. (2023). The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae
Basel : MDPI., 9(8), 910.
https://doi.org/10.3390/horticulturae9080910

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Uzelac B, Subotić A. The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae. 2023;9(8):910.
doi:10.3390/horticulturae9080910 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Uzelac, Branka, Subotić, Angelina, "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro" in Horticulturae, 9, no. 8 (2023):910,
https://doi.org/10.3390/horticulturae9080910 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5827
AB  - In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.
PB  - Taylor & Francis Group
T2  - Plant Signaling & Behavior
T1  - Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress
IS  - 1
VL  - 18
DO  - 10.1080/15592324.2023.2219936
SP  - e2219936
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.",
publisher = "Taylor & Francis Group",
journal = "Plant Signaling & Behavior",
title = "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress",
number = "1",
volume = "18",
doi = "10.1080/15592324.2023.2219936",
pages = "e2219936"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior
Taylor & Francis Group., 18(1), e2219936.
https://doi.org/10.1080/15592324.2023.2219936

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior. 2023;18(1):e2219936.
doi:10.1080/15592324.2023.2219936 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress" in Plant Signaling & Behavior, 18, no. 1 (2023):e2219936,
https://doi.org/10.1080/15592324.2023.2219936 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5762
AB  - Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general.
PB  - Basel: MDPI
T2  - Genes
T1  - Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application
IS  - 5
VL  - 14
DO  - 10.3390/genes14051072
SP  - 1072
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general.",
publisher = "Basel: MDPI",
journal = "Genes",
title = "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application",
number = "5",
volume = "14",
doi = "10.3390/genes14051072",
pages = "1072"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes
Basel: MDPI., 14(5), 1072.
https://doi.org/10.3390/genes14051072

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes. 2023;14(5):1072.
doi:10.3390/genes14051072 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application" in Genes, 14, no. 5 (2023):1072,
https://doi.org/10.3390/genes14051072 . .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5763
AB  - This research evaluated the effects of foliarly applied elicitor methyl jasmonate (MeJA) on the potential improvement of drought tolerance in Impatiens walleriana. Growth and leaf physiology parameters (Stomatal conductance, Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index - NBI), as well the expression of four aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1), were evaluated. These parameters could serve as indicators of drought tolerance in I. walleriana, a worldwide popular horticultural plant, very sensitive to drought. The experiment included four treatments: control plants, drought-stressed plants foliarly sprayed with distilled water, drought-stressed plants foliarly sprayed with 5 μM MeJA, and drought-stressed plants foliarly sprayed with 50 μM MeJA. Foliar spraying was performed seven days before drought induction, and on the day of drought stress induction. The stressed plant groups were non-irrigated to reach 15 and 5% soil water content (SWC), while control plants were well-watered (35-37% SWC) during the entire experiment. Drought reduced fresh and dry shoot weight, as well total leaf area but dry matter content was not affected. Foliarly applied MeJA improveda growth parameters, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC in plants foliarly sprayed with MeJA regardless of the used concentration. Similarly, the flavonoid index was reduced at 15 and 5% SWC when 50 μM MeJA was foliarly applied, while there were no observed changes in the anthocyanin index in any treatment. The foliar application of 50 μM MeJA increased I. walleriana chlorophyll index and NBI at 5% SWC, indicating an elicitor contribution to plant drought tolerance at physiological level. Among the four analyzed aquaporin genes, expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants foliarly pre-treated with 50 μM MeJA, indicating an improvement of water flow through cells to maintain homeostasis.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina
T1  - Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5763
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "This research evaluated the effects of foliarly applied elicitor methyl jasmonate (MeJA) on the potential improvement of drought tolerance in Impatiens walleriana. Growth and leaf physiology parameters (Stomatal conductance, Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index - NBI), as well the expression of four aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1), were evaluated. These parameters could serve as indicators of drought tolerance in I. walleriana, a worldwide popular horticultural plant, very sensitive to drought. The experiment included four treatments: control plants, drought-stressed plants foliarly sprayed with distilled water, drought-stressed plants foliarly sprayed with 5 μM MeJA, and drought-stressed plants foliarly sprayed with 50 μM MeJA. Foliar spraying was performed seven days before drought induction, and on the day of drought stress induction. The stressed plant groups were non-irrigated to reach 15 and 5% soil water content (SWC), while control plants were well-watered (35-37% SWC) during the entire experiment. Drought reduced fresh and dry shoot weight, as well total leaf area but dry matter content was not affected. Foliarly applied MeJA improveda growth parameters, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC in plants foliarly sprayed with MeJA regardless of the used concentration. Similarly, the flavonoid index was reduced at 15 and 5% SWC when 50 μM MeJA was foliarly applied, while there were no observed changes in the anthocyanin index in any treatment. The foliar application of 50 μM MeJA increased I. walleriana chlorophyll index and NBI at 5% SWC, indicating an elicitor contribution to plant drought tolerance at physiological level. Among the four analyzed aquaporin genes, expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants foliarly pre-treated with 50 μM MeJA, indicating an improvement of water flow through cells to maintain homeostasis.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina",
title = "Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5763"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_5763

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina. 2023;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_5763 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana" in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina (2023):141,
https://hdl.handle.net/21.15107/rcub_ibiss_5763 .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5335
AB  - Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.
PB  - Basel: MDPI
T2  - Life
T1  - Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro
IS  - 1
VL  - 13
DO  - 10.3390/life13010154
SP  - 154
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2023",
abstract = "Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro",
number = "1",
volume = "13",
doi = "10.3390/life13010154",
pages = "154"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2023). Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life
Basel: MDPI., 13(1), 154.
https://doi.org/10.3390/life13010154

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life. 2023;13(1):154.
doi:10.3390/life13010154 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro" in Life, 13, no. 1 (2023):154,
https://doi.org/10.3390/life13010154 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5307
AB  - This study was performed to determine the response of Impatiens walleriana, an important horticultural plant, to exogenous
application of methyl jasmonate (MeJA) during in vitro PEG8000−induced water defcit. Shoots were pretreated with graded
MeJA concentrations (0, 5, 50, and 100 µM) in Murashige and Skoog (MS) medium for 7 days. After pretreatment, shoots
were exposed to diferent treatments for 20 days: MS with polyethylene glycol (PEG8000 −0 or 3%), MS with PEG8000
(3%)+MeJA (5, 50 or 100 µM), and MS with MeJA (5, 50 or 100 µM) only. MeJA pretreatment had positive efects on I.
walleriana growth (fresh weight, plant height, number of leaves per plant and proliferation rate) in non-stressed shoots,
especially after application of the lowest MeJA concentration (5 µM). In the water defcit conditions caused by PEG8000, pretreatment with 5 µM MeJA increased all analyzed growth parameters, and caused decreased photosynthetic pigment, proline
and total amino acid content, total polyphenol and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity, hydrogen peroxide and
malondialdehyde content. Additionally, changes in the activity of antioxidant enzymes (superoxide dismutase, peroxidase
and catalase) were induced. Pretreatment with higher MeJA concentrations (50 and 100 µM) mainly suppressed the I. walleriana shoots growth under water defcit, combination treatments and treatment only with MeJA. These results indicate the
potential of MeJA’s in enhancing I. walleriana ability to withstand the water stress in vitro. I. walleriana improved water
stress resistance by promoting defense-related metabolism, which was mainly induced by pretreatment with the lowest
applied MeJA concentration (5 µM).
PB  - Springer Nature B.V. 2022
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate
DO  - 10.1007/s11240-022-02432-z
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2022",
abstract = "This study was performed to determine the response of Impatiens walleriana, an important horticultural plant, to exogenous
application of methyl jasmonate (MeJA) during in vitro PEG8000−induced water defcit. Shoots were pretreated with graded
MeJA concentrations (0, 5, 50, and 100 µM) in Murashige and Skoog (MS) medium for 7 days. After pretreatment, shoots
were exposed to diferent treatments for 20 days: MS with polyethylene glycol (PEG8000 −0 or 3%), MS with PEG8000
(3%)+MeJA (5, 50 or 100 µM), and MS with MeJA (5, 50 or 100 µM) only. MeJA pretreatment had positive efects on I.
walleriana growth (fresh weight, plant height, number of leaves per plant and proliferation rate) in non-stressed shoots,
especially after application of the lowest MeJA concentration (5 µM). In the water defcit conditions caused by PEG8000, pretreatment with 5 µM MeJA increased all analyzed growth parameters, and caused decreased photosynthetic pigment, proline
and total amino acid content, total polyphenol and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity, hydrogen peroxide and
malondialdehyde content. Additionally, changes in the activity of antioxidant enzymes (superoxide dismutase, peroxidase
and catalase) were induced. Pretreatment with higher MeJA concentrations (50 and 100 µM) mainly suppressed the I. walleriana shoots growth under water defcit, combination treatments and treatment only with MeJA. These results indicate the
potential of MeJA’s in enhancing I. walleriana ability to withstand the water stress in vitro. I. walleriana improved water
stress resistance by promoting defense-related metabolism, which was mainly induced by pretreatment with the lowest
applied MeJA concentration (5 µM).",
publisher = "Springer Nature B.V. 2022",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate",
doi = "10.1007/s11240-022-02432-z"
}

Đurić, M., Subotić, A., Trifunović-Momčilov, M.,& Milošević, S.. (2022). Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate. in Plant Cell, Tissue and Organ Culture (PCTOC)
Springer Nature B.V. 2022..
https://doi.org/10.1007/s11240-022-02432-z

Đurić M, Subotić A, Trifunović-Momčilov M, Milošević S. Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate. in Plant Cell, Tissue and Organ Culture (PCTOC). 2022;.
doi:10.1007/s11240-022-02432-z .

Đurić, Marija, Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, "Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate" in Plant Cell, Tissue and Organ Culture (PCTOC) (2022),
https://doi.org/10.1007/s11240-022-02432-z . .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Trifunović-Momčilov, Milana
AU  - Đurić, Marija
AU  - Koruga, Đuro
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5145
AB  - The rapid production and numerous applications of nanomaterials warrant the necessity
and importance of examining nanoparticles in terms to their environmental and biological effects
and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified
fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and
fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment,
and aquaporin genes expression in cherry tomato plants were studied after during short- and longterm continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic
pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of
cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased
aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstanceexposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance
showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in
fruits and may be considered as a promising nanofertilizer.
PB  - Basel: MDPI
T2  - Plants
T1  - Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex
IS  - 21
VL  - 11
DO  - 10.3390/plants11212810
SP  - 2810
ER  - 

@article{
author = "Subotić, Angelina and Jevremović, Slađana and Milošević, Snežana and Trifunović-Momčilov, Milana and Đurić, Marija and Koruga, Đuro",
year = "2022",
abstract = "The rapid production and numerous applications of nanomaterials warrant the necessity
and importance of examining nanoparticles in terms to their environmental and biological effects
and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified
fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and
fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment,
and aquaporin genes expression in cherry tomato plants were studied after during short- and longterm continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic
pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of
cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased
aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstanceexposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance
showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in
fruits and may be considered as a promising nanofertilizer.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex",
number = "21",
volume = "11",
doi = "10.3390/plants11212810",
pages = "2810"
}

Subotić, A., Jevremović, S., Milošević, S., Trifunović-Momčilov, M., Đurić, M.,& Koruga, Đ.. (2022). Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex. in Plants
Basel: MDPI., 11(21), 2810.
https://doi.org/10.3390/plants11212810

Subotić A, Jevremović S, Milošević S, Trifunović-Momčilov M, Đurić M, Koruga Đ. Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex. in Plants. 2022;11(21):2810.
doi:10.3390/plants11212810 .

Subotić, Angelina, Jevremović, Slađana, Milošević, Snežana, Trifunović-Momčilov, Milana, Đurić, Marija, Koruga, Đuro, "Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex" in Plants, 11, no. 21 (2022):2810,
https://doi.org/10.3390/plants11212810 . .

TY  - CONF
AU  - Đurić, Marija
AU  - Stamenković, Nikola
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5327
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5327
ER  - 

@conference{
author = "Đurić, Marija and Stamenković, Nikola and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5327"
}

Đurić, M., Stamenković, N., Trifunović-Momčilov, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327

Đurić M, Stamenković N, Trifunović-Momčilov M, Milošević S, Marković M, Giba Z, Subotić A. The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

Đurić, Marija, Stamenković, Nikola, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):60,
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5377
AB  - Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl.
AB  - Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом
T1  - Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5377
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl., Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом, Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5377"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

TY  - CONF
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Marković, Marija
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5398
AB  - Лепи Јова (Impatiens walleriana) је веома популарна хортикултурна врста у Србији 
и свету због својих декоративних особина, дугог периода цветања и широког 
спектра боје цветова. У његовој комерцијалној производњи наводњавање је 
смањено током транспорта и пласмана биљака на тржиште. Суша има неповољан 
утицај на растење и развиће биљака, а у ћелијама доводи до успоравања 
анаболичких и убрзавања катаболичких процеса, уз појачану производњу 
реактивних форми кисеоника (Reactive oxygen species – ROS). Дисбаланс између 
настанка и уклањања ROS има за последицу нарушавање ћелијске хомеостазе и 
оксидативни стрес. Иако ROS имају важну сигналну улогу, њихова висока 
цитотоксичност и реактивност доводе до модификација DNK, RNK, протеина и 
липида мембрана. У овом истраживању испитиван је утицај натријум 
нитропрусида (Sodium nitroprusside – SNP), као донора NO, на физиолошке и 
биохемијске промене in vitro гајеног Лепог Јове у условима суше изазване 
полиетилен гликолом (PEG8000). Изданци (величине 20-25 мм) су гајени на 
хранљивим подлогама са SNP (0, 50, 100 и 250 μM) 20 дана. Након претретмана, 
изданци су пребачени на свеже MS подлоге којима су додати PEG8000 (0 или 3%) 
и/или SNP (0, 50, 100 или 250 μM) и гајени су седам дана. Утицај егзогене примене 
SNP на повећање толеранције Лепог Јове на сушу испољава се у променама 
концентрације H2O2, малондиалдехида и укупних фенолних једињења, као и 
активности актиоксидативних ензима: каталаза, пероксидаза и супероксиддисмутаза у изданцима.
AB  - Lepi Jova (Impatiens walleriana) je veoma popularna hortikulturna vrsta u Srbiji i svetu zbog svojih dekorativnih osobina, dugog perioda cvetanja i širokog spektra boje cvetova. U njegovoj komercijalnoj proizvodnji navodnjavanje je smanjeno tokom transporta i plasmana biljaka na tržište. Suša ima nepovoljan uticaj na rastenje i razviće biljaka, a u ćelijama dovodi do usporavanja anaboličkih i ubrzavanja kataboličkih procesa, uz pojačanu proizvodnju reaktivnih formi kiseonika (Reactive oxygen species – ROS). Disbalans između nastanka i uklanjanja ROS ima za posledicu narušavanje ćelijske homeostaze i oksidativni stres. Iako ROS imaju važnu signalnu ulogu, njihova visoka citotoksičnost i reaktivnost dovode do modifikacija DNK, RNK, proteina i lipida membrana. U ovom istraživanju ispitivan je uticaj natrijum nitroprusida (Sodium nitroprusside – SNP), kao donora NO, na fiziološke i biohemijske promene in vitro gajenog Lepog Jove u uslovima suše izazvane polietilen glikolom (PEG8000). Izdanci (veličine 20-25 mm) su gajeni na hranljivim podlogama sa SNP (0, 50, 100 i 250 μM) 20 dana. Nakon pretretmana, izdanci su prebačeni na sveže MS podloge kojima su dodati PEG8000 (0 ili 3%) i/ili SNP (0, 50, 100 ili 250 μM) i gajeni su sedam dana. Uticaj egzogene primene SNP na povećanje tolerancije Lepog Jove na sušu ispoljava se u promenama koncentracije H2O2, malondialdehida i ukupnih fenolnih jedinjenja, kao i aktivnosti aktioksidativnih enzima: katalaza, peroksidaza i superoksiddismutaza u izdancima.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro
T1  - Uticaj natrijum nitroprusida na fiziološki odgovor Lepog Jove (Impatiens walleriana) u uslovima suše izazvane polietilen glikolom in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5398
ER  - 

@conference{
author = "Milošević, Snežana and Đurić, Marija and Trifunović-Momčilov, Milana and Stamenković, Nikola and Marković, Marija and Subotić, Angelina",
year = "2022",
abstract = "Лепи Јова (Impatiens walleriana) је веома популарна хортикултурна врста у Србији 
и свету због својих декоративних особина, дугог периода цветања и широког 
спектра боје цветова. У његовој комерцијалној производњи наводњавање је 
смањено током транспорта и пласмана биљака на тржиште. Суша има неповољан 
утицај на растење и развиће биљака, а у ћелијама доводи до успоравања 
анаболичких и убрзавања катаболичких процеса, уз појачану производњу 
реактивних форми кисеоника (Reactive oxygen species – ROS). Дисбаланс између 
настанка и уклањања ROS има за последицу нарушавање ћелијске хомеостазе и 
оксидативни стрес. Иако ROS имају важну сигналну улогу, њихова висока 
цитотоксичност и реактивност доводе до модификација DNK, RNK, протеина и 
липида мембрана. У овом истраживању испитиван је утицај натријум 
нитропрусида (Sodium nitroprusside – SNP), као донора NO, на физиолошке и 
биохемијске промене in vitro гајеног Лепог Јове у условима суше изазване 
полиетилен гликолом (PEG8000). Изданци (величине 20-25 мм) су гајени на 
хранљивим подлогама са SNP (0, 50, 100 и 250 μM) 20 дана. Након претретмана, 
изданци су пребачени на свеже MS подлоге којима су додати PEG8000 (0 или 3%) 
и/или SNP (0, 50, 100 или 250 μM) и гајени су седам дана. Утицај егзогене примене 
SNP на повећање толеранције Лепог Јове на сушу испољава се у променама 
концентрације H2O2, малондиалдехида и укупних фенолних једињења, као и 
активности актиоксидативних ензима: каталаза, пероксидаза и супероксиддисмутаза у изданцима., Lepi Jova (Impatiens walleriana) je veoma popularna hortikulturna vrsta u Srbiji i svetu zbog svojih dekorativnih osobina, dugog perioda cvetanja i širokog spektra boje cvetova. U njegovoj komercijalnoj proizvodnji navodnjavanje je smanjeno tokom transporta i plasmana biljaka na tržište. Suša ima nepovoljan uticaj na rastenje i razviće biljaka, a u ćelijama dovodi do usporavanja anaboličkih i ubrzavanja kataboličkih procesa, uz pojačanu proizvodnju reaktivnih formi kiseonika (Reactive oxygen species – ROS). Disbalans između nastanka i uklanjanja ROS ima za posledicu narušavanje ćelijske homeostaze i oksidativni stres. Iako ROS imaju važnu signalnu ulogu, njihova visoka citotoksičnost i reaktivnost dovode do modifikacija DNK, RNK, proteina i lipida membrana. U ovom istraživanju ispitivan je uticaj natrijum nitroprusida (Sodium nitroprusside – SNP), kao donora NO, na fiziološke i biohemijske promene in vitro gajenog Lepog Jove u uslovima suše izazvane polietilen glikolom (PEG8000). Izdanci (veličine 20-25 mm) su gajeni na hranljivim podlogama sa SNP (0, 50, 100 i 250 μM) 20 dana. Nakon pretretmana, izdanci su prebačeni na sveže MS podloge kojima su dodati PEG8000 (0 ili 3%) i/ili SNP (0, 50, 100 ili 250 μM) i gajeni su sedam dana. Uticaj egzogene primene SNP na povećanje tolerancije Lepog Jove na sušu ispoljava se u promenama koncentracije H2O2, malondialdehida i ukupnih fenolnih jedinjenja, kao i aktivnosti aktioksidativnih enzima: katalaza, peroksidaza i superoksiddismutaza u izdancima.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro, Uticaj natrijum nitroprusida na fiziološki odgovor Lepog Jove (Impatiens walleriana) u uslovima suše izazvane polietilen glikolom in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5398"
}

Milošević, S., Đurić, M., Trifunović-Momčilov, M., Stamenković, N., Marković, M.,& Subotić, A.. (2022). Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5398

Milošević S, Đurić M, Trifunović-Momčilov M, Stamenković N, Marković M, Subotić A. Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5398 .

Milošević, Snežana, Đurić, Marija, Trifunović-Momčilov, Milana, Stamenković, Nikola, Marković, Marija, Subotić, Angelina, "Утицај натријум нитропрусида на физиолошки одговор Лепог Јове  (Impatiens walleriana) у условима суше изазване полиетилен  гликолом in vitro" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5398 .

TY  - CONF
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Đurić, Marija
AU  - Koruga, Đuro
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5395
AB  - Нанотехнологија је релативно нова научна област и неопходна су темељнија 
истраживања да би се помоћу ње могло доћи до корисних сазнања за добробит 
човечанства. Многе земље у свету су свесне потенцијала који пружа примена 
нанотехнологије у пољопривреди и развијају програме који би омогућили 
повећање приноса, заштиту околине од прекомерне употребе хербицида и 
пестицида и производњу функционалне хране. Циљ ових истраживања је био да се 
испита ефекат 200 mg/l 3HWFC+
наносупстанце (патент PCT/EP2019/083307) на 
процес клијања, морфолошке и физиолошке аспекте развића плодова парадајза. У 
фази развића клијанаца успешно је стимулисан пораст биомасе у присуству 
наносупстанце. У овом раду приказан је и позитиван утицај наносупстанце на 
развиће плодова парадајза и њихов квалитет. У погледу квалитета плодова, 
најбољи резултат ефеката наносусптанце огледао се у повећању садржаја ликопена 
код зрелих плодова у односу на плодове контролних биљака. Резултати ових 
истраживања указују на нови научни допринос разумевању ефекта 3HFWC+
наносупстанце на развојне процесе парадајза и такође имају потенцијални значај у 
унапређењу технологије производње ове повртарске културе, односно добијању 
плодова са повећаним садржајем ликопена.
AB  - Nanotehnologija je relativno nova naučna oblast i neophodna su temeljnija istraživanja da bi se pomoću nje moglo doći do korisnih saznanja za dobrobit čovečanstva. Mnoge zemlje u svetu su svesne potencijala koji pruža primena nanotehnologije u poljoprivredi i razvijaju programe koji bi omogućili povećanje prinosa, zaštitu okoline od prekomerne upotrebe herbicida i pesticida i proizvodnju funkcionalne hrane. Cilj ovih istraživanja je bio da se ispita efekat 200 mg/l 3HWFC+ nanosupstance (patent PCT/EP2019/083307) na proces klijanja, morfološke i fiziološke aspekte razvića plodova paradajza. U fazi razvića klijanaca uspešno je stimulisan porast biomase u prisustvu nanosupstance. U ovom radu prikazan je i pozitivan uticaj nanosupstance na razviće plodova paradajza i njihov kvalitet. U pogledu kvaliteta plodova, najbolji rezultat efekata nanosusptance ogledao se u povećanju sadržaja likopena kod zrelih plodova u odnosu na plodove kontrolnih biljaka. Rezultati ovih istraživanja ukazuju na novi naučni doprinos razumevanju efekta 3HFWC+ nanosupstance na razvojne procese paradajza i takođe imaju potencijalni značaj u unapređenju tehnologije proizvodnje ove povrtarske kulture, odnosno dobijanju plodova sa povećanim sadržajem likopena.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.)
T1  - Efekat drugog derivata molekula S60, 3HFWC+ nanosupstance, na morfološke i fiziološke aspekte razvića paradajza (Solanum lycopersicum L.)
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5395
ER  - 

@conference{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana and Jevremović, Slađana and Đurić, Marija and Koruga, Đuro",
year = "2022",
abstract = "Нанотехнологија је релативно нова научна област и неопходна су темељнија 
истраживања да би се помоћу ње могло доћи до корисних сазнања за добробит 
човечанства. Многе земље у свету су свесне потенцијала који пружа примена 
нанотехнологије у пољопривреди и развијају програме који би омогућили 
повећање приноса, заштиту околине од прекомерне употребе хербицида и 
пестицида и производњу функционалне хране. Циљ ових истраживања је био да се 
испита ефекат 200 mg/l 3HWFC+
наносупстанце (патент PCT/EP2019/083307) на 
процес клијања, морфолошке и физиолошке аспекте развића плодова парадајза. У 
фази развића клијанаца успешно је стимулисан пораст биомасе у присуству 
наносупстанце. У овом раду приказан је и позитиван утицај наносупстанце на 
развиће плодова парадајза и њихов квалитет. У погледу квалитета плодова, 
најбољи резултат ефеката наносусптанце огледао се у повећању садржаја ликопена 
код зрелих плодова у односу на плодове контролних биљака. Резултати ових 
истраживања указују на нови научни допринос разумевању ефекта 3HFWC+
наносупстанце на развојне процесе парадајза и такође имају потенцијални значај у 
унапређењу технологије производње ове повртарске културе, односно добијању 
плодова са повећаним садржајем ликопена., Nanotehnologija je relativno nova naučna oblast i neophodna su temeljnija istraživanja da bi se pomoću nje moglo doći do korisnih saznanja za dobrobit čovečanstva. Mnoge zemlje u svetu su svesne potencijala koji pruža primena nanotehnologije u poljoprivredi i razvijaju programe koji bi omogućili povećanje prinosa, zaštitu okoline od prekomerne upotrebe herbicida i pesticida i proizvodnju funkcionalne hrane. Cilj ovih istraživanja je bio da se ispita efekat 200 mg/l 3HWFC+ nanosupstance (patent PCT/EP2019/083307) na proces klijanja, morfološke i fiziološke aspekte razvića plodova paradajza. U fazi razvića klijanaca uspešno je stimulisan porast biomase u prisustvu nanosupstance. U ovom radu prikazan je i pozitivan uticaj nanosupstance na razviće plodova paradajza i njihov kvalitet. U pogledu kvaliteta plodova, najbolji rezultat efekata nanosusptance ogledao se u povećanju sadržaja likopena kod zrelih plodova u odnosu na plodove kontrolnih biljaka. Rezultati ovih istraživanja ukazuju na novi naučni doprinos razumevanju efekta 3HFWC+ nanosupstance na razvojne procese paradajza i takođe imaju potencijalni značaj u unapređenju tehnologije proizvodnje ove povrtarske kulture, odnosno dobijanju plodova sa povećanim sadržajem likopena.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.), Efekat drugog derivata molekula S60, 3HFWC+ nanosupstance, na morfološke i fiziološke aspekte razvića paradajza (Solanum lycopersicum L.)",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5395"
}

Subotić, A., Trifunović-Momčilov, M., Milošević, S., Jevremović, S., Đurić, M.,& Koruga, Đ.. (2022). Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 49.
https://hdl.handle.net/21.15107/rcub_ibiss_5395

Subotić A, Trifunović-Momčilov M, Milošević S, Jevremović S, Đurić M, Koruga Đ. Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:49.
https://hdl.handle.net/21.15107/rcub_ibiss_5395 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, Jevremović, Slađana, Đurić, Marija, Koruga, Đuro, "Ефекат другог деривата молекула С60, 3HFWC+ наносупстанце, на  морфолошке и физиолошке аспекте развића парадајза (Solanum lycopersicum L.)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):49,
https://hdl.handle.net/21.15107/rcub_ibiss_5395 .

TY  - CONF
AU  - Subotić, Angelina
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Jerinić-Prodanović, Dušanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5396
AB  - Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес.
AB  - Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica
T1  - Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5396
ER  - 

@conference{
author = "Subotić, Angelina and Trifunović-Momčilov, Milana and Milošević, Snežana and Đurić, Marija and Jevremović, Slađana and Jerinić-Prodanović, Dušanka",
year = "2022",
abstract = "Биотички стрес, резултат интеракције између биљке и другог живог организма, 
укључујући и инсекте, има различите негативне ефекте на растење и развиће 
биљака. Циљ овог истраживања је био да се испитају физиолошки и биохемијски аспекти одговора биљака шаргарепе (Daucus carota) на стрес изаван исхраном 
мрквине буве (Bactericera trigonica). Поред директне исхране на биљкама, B.
trigonica је и вектор фастидиозне бактерије Candidatus Liberibacter solanacearum
која проузрокује различите болести код биљака из фамилије Apiaceae. Од 
одгајених имага B. trigonica изоловани су парови од по пет мужјака и женки, а 
потом су наношени на биљке шаргарепе које су претходно гајене у контролисаним условима. Делови листова биљака у експерименту узорковани су након једне и четири недеље, од инокулације инсеката, у циљу одређивања физиолошких и биохемијских одговора на биотички стрес. Код биљака шаргарепе, услед стреса насталог исхраном лисне буве, уочен је измењен садржај фотосинтетичких пигмената. Хистохемијском локализацијом праћена је продукција реактивних форми кисеоника у листовима инокулисаних биљака шаргарепе. Разлике у одговору појединих компоненти антиоксидативног метаболизма изаваних исхраном лисне буве могу бити адаптивна стратегија шаргарепе у одговору на примењени биотички стрес., Biotički stres, rezultat interakcije između biljke i drugog živog organizma, uključujući i insekte, ima različite negativne efekte na rastenje i razviće biljaka. Cilj ovog istraživanja je bio da se ispitaju fiziološki i biohemijski aspekti odgovora biljaka šargarepe (Daucus carota) na stres izavan ishranom mrkvine buve (Bactericera trigonica). Pored direktne ishrane na biljkama, B. trigonica je i vektor fastidiozne bakterije Candidatus Liberibacter solanacearum koja prouzrokuje različite bolesti kod biljaka iz familije Apiaceae. Od odgajenih imaga B. trigonica izolovani su parovi od po pet mužjaka i ženki, a potom su nanošeni na biljke šargarepe koje su prethodno gajene u kontrolisanim uslovima. Delovi listova biljaka u eksperimentu uzorkovani su nakon jedne i četiri nedelje, od inokulacije insekata, u cilju određivanja fizioloških i biohemijskih odgovora na biotički stres. Kod biljaka šargarepe, usled stresa nastalog ishranom lisne buve, uočen je izmenjen sadržaj fotosintetičkih pigmenata. Histohemijskom lokalizacijom praćena je produkcija reaktivnih formi kiseonika u listovima inokulisanih biljaka šargarepe. Razlike u odgovoru pojedinih komponenti antioksidativnog metabolizma izavanih ishranom lisne buve mogu biti adaptivna strategija šargarepe u odgovoru na primenjeni biotički stres.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica, Fiziološki i biohemijski aspekti odgovora biljaka šargarepe na biotički stres izazvan ishranom mrkvine lisne buve Bactericera trigonica",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5396"
}

Subotić, A., Trifunović-Momčilov, M., Milošević, S., Đurić, M., Jevremović, S.,& Jerinić-Prodanović, D.. (2022). Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396

Subotić A, Trifunović-Momčilov M, Milošević S, Đurić M, Jevremović S, Jerinić-Prodanović D. Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:48.
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

Subotić, Angelina, Trifunović-Momčilov, Milana, Milošević, Snežana, Đurić, Marija, Jevremović, Slađana, Jerinić-Prodanović, Dušanka, "Физиолошки и биохемијски аспекти одговора биљака шаргарепе  на биотички стрес изазван исхраном мрквине лисне буве  Bactericera trigonica" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):48,
https://hdl.handle.net/21.15107/rcub_ibiss_5396 .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5363
AB  - Суша представља ограничавајући фактор у производњи комерцијално значајних
биљних врста, испољавајући негативне ефекте у свим развојним стадијумима. У
овом истраживању испитиван је утицај фолијарне примене елицитора метил
јасмоната (МеЈА) на растење и физиолошки одговор Лепог Јове (Impatiens
walleriana) при суши. Лепи Јова се убраја у најпопуларније хортикултурне врсте
широм света, а ограничавајући фактор у производњи, транспорту и пласману
биљака на тржишту представља дефицит воде. Елицитација подразумева примену
једињења у циљу индуковања сигналних путева повезаних са одбраном биљака од
стреса, а у ту сврху често се користе регулатори растења попут МеЈА. Фолијарно
третирање биљака МеЈА (5 и 50 μМ) вршено је два пута током експерименталног
рада: седам дана пре индуковања суше и на дан индукције суше. Биљке су
подељене у групе према третманима, а суша је индукована тако што биљке нису
заливане до достизања жељених процената влажности супстрата (15 и 5%). Оба
третмана суше укључивала су, поред контролних биљака (35-37% влажности
супстрата) и биљке које су постепено рехидратисане – заливане четири дана након
исушивања до достизања контролних вредности влажности супстрата. Забележен
је позитиван утицај МеЈА на параметре растења: масу свежих и сувих изданака,
као и укупну површину листова Лепог Јове. Код група биљака третираних МеЈА,
забележен је пад у концентрацији показатеља оксидативног стреса –
малондиалдехида и H2О2 при суши, као и мањи садржај полифенола и флавонола.
AB  - Suša predstavlja ograničavajući faktor u proizvodnji komercijalno značajnih biljnih vrsta, ispoljavajući negativne efekte u svim razvojnim stadijumima. U ovom istraživanju ispitivan je uticaj folijarne primene elicitora metil jasmonata (MeJA) na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri suši. Lepi Jova se ubraja u najpopularnije hortikulturne vrste širom sveta, a ograničavajući faktor u proizvodnji, transportu i plasmanu biljaka na tržištu predstavlja deficit vode. Elicitacija podrazumeva primenu jedinjenja u cilju indukovanja signalnih puteva povezanih sa odbranom biljaka od stresa, a u tu svrhu često se koriste regulatori rastenja poput MeJA. Folijarno tretiranje biljaka MeJA (5 i 50 μM) vršeno je dva puta tokom eksperimentalnog rada: sedam dana pre indukovanja suše i na dan indukcije suše. Biljke su podeljene u grupe prema tretmanima, a suša je indukovana tako što biljke nisu zalivane do dostizanja željenih procenata vlažnosti supstrata (15 i 5%). Oba tretmana suše uključivala su, pored kontrolnih biljaka (35-37% vlažnosti supstrata) i biljke koje su postepeno rehidratisane – zalivane četiri dana nakon isušivanja do dostizanja kontrolnih vrednosti vlažnosti supstrata. Zabeležen je pozitivan uticaj MeJA na parametre rastenja: masu svežih i suvih izdanaka, kao i ukupnu površinu listova Lepog Jove. Kod grupa biljaka tretiranih MeJA, zabeležen je pad u koncentraciji pokazatelja oksidativnog stresa – malondialdehida i H2O2 pri suši, kao i manji sadržaj polifenola i flavonola.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши
T1  - Uticaj folijarne primene metil jasmonata na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri indukovanoj suši
IS  - 048
VL  - 57
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5363
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2022",
abstract = "Суша представља ограничавајући фактор у производњи комерцијално значајних
биљних врста, испољавајући негативне ефекте у свим развојним стадијумима. У
овом истраживању испитиван је утицај фолијарне примене елицитора метил
јасмоната (МеЈА) на растење и физиолошки одговор Лепог Јове (Impatiens
walleriana) при суши. Лепи Јова се убраја у најпопуларније хортикултурне врсте
широм света, а ограничавајући фактор у производњи, транспорту и пласману
биљака на тржишту представља дефицит воде. Елицитација подразумева примену
једињења у циљу индуковања сигналних путева повезаних са одбраном биљака од
стреса, а у ту сврху често се користе регулатори растења попут МеЈА. Фолијарно
третирање биљака МеЈА (5 и 50 μМ) вршено је два пута током експерименталног
рада: седам дана пре индуковања суше и на дан индукције суше. Биљке су
подељене у групе према третманима, а суша је индукована тако што биљке нису
заливане до достизања жељених процената влажности супстрата (15 и 5%). Оба
третмана суше укључивала су, поред контролних биљака (35-37% влажности
супстрата) и биљке које су постепено рехидратисане – заливане четири дана након
исушивања до достизања контролних вредности влажности супстрата. Забележен
је позитиван утицај МеЈА на параметре растења: масу свежих и сувих изданака,
као и укупну површину листова Лепог Јове. Код група биљака третираних МеЈА,
забележен је пад у концентрацији показатеља оксидативног стреса –
малондиалдехида и H2О2 при суши, као и мањи садржај полифенола и флавонола., Suša predstavlja ograničavajući faktor u proizvodnji komercijalno značajnih biljnih vrsta, ispoljavajući negativne efekte u svim razvojnim stadijumima. U ovom istraživanju ispitivan je uticaj folijarne primene elicitora metil jasmonata (MeJA) na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri suši. Lepi Jova se ubraja u najpopularnije hortikulturne vrste širom sveta, a ograničavajući faktor u proizvodnji, transportu i plasmanu biljaka na tržištu predstavlja deficit vode. Elicitacija podrazumeva primenu jedinjenja u cilju indukovanja signalnih puteva povezanih sa odbranom biljaka od stresa, a u tu svrhu često se koriste regulatori rastenja poput MeJA. Folijarno tretiranje biljaka MeJA (5 i 50 μM) vršeno je dva puta tokom eksperimentalnog rada: sedam dana pre indukovanja suše i na dan indukcije suše. Biljke su podeljene u grupe prema tretmanima, a suša je indukovana tako što biljke nisu zalivane do dostizanja željenih procenata vlažnosti supstrata (15 i 5%). Oba tretmana suše uključivala su, pored kontrolnih biljaka (35-37% vlažnosti supstrata) i biljke koje su postepeno rehidratisane – zalivane četiri dana nakon isušivanja do dostizanja kontrolnih vrednosti vlažnosti supstrata. Zabeležen je pozitivan uticaj MeJA na parametre rastenja: masu svežih i suvih izdanaka, kao i ukupnu površinu listova Lepog Jove. Kod grupa biljaka tretiranih MeJA, zabeležen je pad u koncentraciji pokazatelja oksidativnog stresa – malondialdehida i H2O2 pri suši, kao i manji sadržaj polifenola i flavonola.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши, Uticaj folijarne primene metil jasmonata na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri indukovanoj suši",
number = "048",
volume = "57",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5363"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2022). Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 57(048), 64.
https://hdl.handle.net/21.15107/rcub_ibiss_5363

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;57(048):64.
https://hdl.handle.net/21.15107/rcub_ibiss_5363 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia, 57, no. 048 (2022):64,
https://hdl.handle.net/21.15107/rcub_ibiss_5363 .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://doi.org/10.3390/plants10050902
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4228
AB  - In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.
PB  - MDPI AG
T2  - Plants
T1  - Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters
IS  - 5
VL  - 10
DO  - 10.3390/plants10050902
SP  - 902
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Jevremović, Slađana and Subotić, Angelina",
year = "2021",
abstract = "In ornamental geophytes, conventional vegetative propagation is not economically feasible due to very slow development and ineffective methods. It can take several years until a new plant is formed and commercial profitability is achieved. Therefore, micropropagation techniques have been developed to increase the multiplication rate and thus shorten the multiplication and regeneration period. The majority of these techniques rely on the formation of new bulbs and their sprouting. Dormancy is one of the main limiting factors to speed up multiplication in vitro. Bulbous species have a period of bulb dormancy which enables them to survive unfavorable natural conditions. Bulbs grown in vitro also exhibit dormancy, which has to be overcome in order to allow sprouting of bulbs in the next vegetation period. During the period of dormancy, numerous physiological processes occur, many of which have not been elucidated yet. Understanding the process of dormancy will allow us to speed up and improve breeding of geophytes and thereby achieve economic profitability, which is very important for horticulture. This review focuses on recent findings in the area of bulb dormancy initiation and release in fritillaries, with particular emphasis on the effect of plant growth regulators and low-temperature pretreatment on dormancy release in relation to induction of antioxidative enzymes’ activity in vitro.",
publisher = "MDPI AG",
journal = "Plants",
title = "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters",
number = "5",
volume = "10",
doi = "10.3390/plants10050902",
pages = "902"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Jevremović, S.,& Subotić, A.. (2021). Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants
MDPI AG., 10(5), 902.
https://doi.org/10.3390/plants10050902

Marković M, Trifunović-Momčilov M, Uzelac B, Jevremović S, Subotić A. Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters. in Plants. 2021;10(5):902.
doi:10.3390/plants10050902 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Jevremović, Slađana, Subotić, Angelina, "Bulb dormancy in vitro—fritillaria meleagris: Initiation, release and physiological parameters" in Plants, 10, no. 5 (2021):902,
https://doi.org/10.3390/plants10050902 . .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://link.springer.com/article/10.1007/s11627-020-10143-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4231
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4473
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.
PB  - Springer
T2  - In Vitro Cellular and Developmental Biology - Plant
T1  - Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)
IS  - 3
VL  - 57
DO  - 10.1007/s11627-020-10143-3
SP  - 470
EP  - 480
ER  - 

@article{
author = "Filipović, Biljana and Trifunović-Momčilov, Milana and Simonović, Ana and Jevremović, Slađana and Milošević, Snežana and Subotić, Angelina",
year = "2021",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.",
publisher = "Springer",
journal = "In Vitro Cellular and Developmental Biology - Plant",
title = "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)",
number = "3",
volume = "57",
doi = "10.1007/s11627-020-10143-3",
pages = "470-480"
}

Filipović, B., Trifunović-Momčilov, M., Simonović, A., Jevremović, S., Milošević, S.,& Subotić, A.. (2021). Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant
Springer., 57(3), 470-480.
https://doi.org/10.1007/s11627-020-10143-3

Filipović B, Trifunović-Momčilov M, Simonović A, Jevremović S, Milošević S, Subotić A. Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant. 2021;57(3):470-480.
doi:10.1007/s11627-020-10143-3 .

Filipović, Biljana, Trifunović-Momčilov, Milana, Simonović, Ana, Jevremović, Slađana, Milošević, Snežana, Subotić, Angelina, "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)" in In Vitro Cellular and Developmental Biology - Plant, 57, no. 3 (2021):470-480,
https://doi.org/10.1007/s11627-020-10143-3 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Milošević, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4119
AB  - Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
PB  - Basel, Switzerland: MDPI
T2  - Plants
T1  - Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery
IS  - 1
VL  - 10
DO  - 10.3390/plants10010154
SP  - 154
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Dragićević, Milan and Simonović, Ana and Milošević, Snežana",
year = "2021",
abstract = "Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.",
publisher = "Basel, Switzerland: MDPI",
journal = "Plants",
title = "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery",
number = "1",
volume = "10",
doi = "10.3390/plants10010154",
pages = "154"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Dragićević, M., Simonović, A.,& Milošević, S.. (2021). Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants
Basel, Switzerland: MDPI., 10(1), 154.
https://doi.org/10.3390/plants10010154

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Dragićević M, Simonović A, Milošević S. Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants. 2021;10(1):154.
doi:10.3390/plants10010154 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Dragićević, Milan, Simonović, Ana, Milošević, Snežana, "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery" in Plants, 10, no. 1 (2021):154,
https://doi.org/10.3390/plants10010154 . .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://link.springer.com/article/10.1007/s11627-020-10143-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4231
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.
PB  - Springer
T2  - In Vitro Cellular and Developmental Biology - Plant
T1  - Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)
IS  - 3
VL  - 57
DO  - 10.1007/s11627-020-10143-3
SP  - 470
EP  - 480
ER  - 

@article{
author = "Filipović, Biljana and Trifunović-Momčilov, Milana and Simonović, Ana and Jevremović, Slađana and Milošević, Snežana and Subotić, Angelina",
year = "2021",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.",
publisher = "Springer",
journal = "In Vitro Cellular and Developmental Biology - Plant",
title = "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)",
number = "3",
volume = "57",
doi = "10.1007/s11627-020-10143-3",
pages = "470-480"
}

Filipović, B., Trifunović-Momčilov, M., Simonović, A., Jevremović, S., Milošević, S.,& Subotić, A.. (2021). Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant
Springer., 57(3), 470-480.
https://doi.org/10.1007/s11627-020-10143-3

Filipović B, Trifunović-Momčilov M, Simonović A, Jevremović S, Milošević S, Subotić A. Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant. 2021;57(3):470-480.
doi:10.1007/s11627-020-10143-3 .

Filipović, Biljana, Trifunović-Momčilov, Milana, Simonović, Ana, Jevremović, Slađana, Milošević, Snežana, Subotić, Angelina, "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)" in In Vitro Cellular and Developmental Biology - Plant, 57, no. 3 (2021):470-480,
https://doi.org/10.1007/s11627-020-10143-3 . .

TY  - JOUR
AU  - Paunović, Danijela
AU  - Ćuković, Katarina
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
AU  - Simonović, Ana
AU  - Dragićević, Milan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4392
AB  - Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.
PB  - Basel: MDPI
T2  - Plants
T1  - The Arabinogalactan Protein Family of Centaurium erythraea Rafn
IS  - 9
VL  - 10
DO  - 10.3390/plants10091870
SP  - 1870
ER  - 

@article{
author = "Paunović, Danijela and Ćuković, Katarina and Bogdanović, Milica and Todorović, Slađana and Trifunović-Momčilov, Milana and Subotić, Angelina and Simonović, Ana and Dragićević, Milan",
year = "2021",
abstract = "Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "The Arabinogalactan Protein Family of Centaurium erythraea Rafn",
number = "9",
volume = "10",
doi = "10.3390/plants10091870",
pages = "1870"
}

Paunović, D., Ćuković, K., Bogdanović, M., Todorović, S., Trifunović-Momčilov, M., Subotić, A., Simonović, A.,& Dragićević, M.. (2021). The Arabinogalactan Protein Family of Centaurium erythraea Rafn. in Plants
Basel: MDPI., 10(9), 1870.
https://doi.org/10.3390/plants10091870

Paunović D, Ćuković K, Bogdanović M, Todorović S, Trifunović-Momčilov M, Subotić A, Simonović A, Dragićević M. The Arabinogalactan Protein Family of Centaurium erythraea Rafn. in Plants. 2021;10(9):1870.
doi:10.3390/plants10091870 .

Paunović, Danijela, Ćuković, Katarina, Bogdanović, Milica, Todorović, Slađana, Trifunović-Momčilov, Milana, Subotić, Angelina, Simonović, Ana, Dragićević, Milan, "The Arabinogalactan Protein Family of Centaurium erythraea Rafn" in Plants, 10, no. 9 (2021):1870,
https://doi.org/10.3390/plants10091870 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Đurić, Marija
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4501
AB  - The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro
IS  - 10
VL  - 11
DO  - 10.3390/agronomy11102056
SP  - 2056
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Đurić, Marija and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro",
number = "10",
volume = "11",
doi = "10.3390/agronomy11102056",
pages = "2056"
}

Trifunović-Momčilov, M., Milošević, S., Marković, M., Đurić, M., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy
Basel: MDPI., 11(10), 2056.
https://doi.org/10.3390/agronomy11102056

Trifunović-Momčilov M, Milošević S, Marković M, Đurić M, Jevremović S, Dragićević IČ, Subotić A. Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro. in Agronomy. 2021;11(10):2056.
doi:10.3390/agronomy11102056 .

Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Đurić, Marija, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro" in Agronomy, 11, no. 10 (2021):2056,
https://doi.org/10.3390/agronomy11102056 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dobrev, Petre I.
AU  - Marković, Marija
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4649
AB  - Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro
VL  - 11
DO  - 10.1038/s41598-021-00866-7
SP  - 21471
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dobrev, Petre I. and Marković, Marija and Milošević, Snežana and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro",
volume = "11",
doi = "10.1038/s41598-021-00866-7",
pages = "21471"
}

Trifunović-Momčilov, M., Motyka, V., Dobrev, P. I., Marković, M., Milošević, S., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports
Springer Nature., 11, 21471.
https://doi.org/10.1038/s41598-021-00866-7

Trifunović-Momčilov M, Motyka V, Dobrev PI, Marković M, Milošević S, Jevremović S, Dragićević IČ, Subotić A. Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports. 2021;11:21471.
doi:10.1038/s41598-021-00866-7 .

Trifunović-Momčilov, Milana, Motyka, Václav, Dobrev, Petre I., Marković, Marija, Milošević, Snežana, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro" in Scientific Reports, 11 (2021):21471,
https://doi.org/10.1038/s41598-021-00866-7 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Stanojević, Olja
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/11/1719
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4653
AB  - Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3
IS  - 11
VL  - 10
DO  - 10.3390/antiox10111719
SP  - 1719
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Stanojević, Olja and Vujčić, Zoran and Dojnov, Biljana and Trifunović-Momčilov, Milana and Marković, Marija",
year = "2021",
abstract = "Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3",
number = "11",
volume = "10",
doi = "10.3390/antiox10111719",
pages = "1719"
}

Radulović, O., Stanković, S., Stanojević, O., Vujčić, Z., Dojnov, B., Trifunović-Momčilov, M.,& Marković, M.. (2021). Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants
Basel: MDPI., 10(11), 1719.
https://doi.org/10.3390/antiox10111719

Radulović O, Stanković S, Stanojević O, Vujčić Z, Dojnov B, Trifunović-Momčilov M, Marković M. Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants. 2021;10(11):1719.
doi:10.3390/antiox10111719 .

Radulović, Olga, Stanković, Slaviša, Stanojević, Olja, Vujčić, Zoran, Dojnov, Biljana, Trifunović-Momčilov, Milana, Marković, Marija, "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3" in Antioxidants, 10, no. 11 (2021):1719,
https://doi.org/10.3390/antiox10111719 . .