TY  - JOUR
AU  - Rakić, Dejana
AU  - Joksimović Jović, Jovana
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Sretenović, Jasmina
AU  - Nikolić, Marina
AU  - Jović, Nikola
AU  - Bićanin Ilić, Marija
AU  - Arsenijević, Petar
AU  - Dimitrijević, Aleksandra
AU  - Vulović, Tatjana
AU  - Ristić, Nataša
AU  - Bulatović, Kristina
AU  - Bolevich, Sergej
AU  - Stijak, Lazar
AU  - Pantović, Suzana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6087
AB  - Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.
PB  - Basel: MDPI
T2  - Medicina
T1  - High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats
IS  - 6
VL  - 59
DO  - 10.3390/medicina59061104
SP  - 1104
ER  - 

@article{
author = "Rakić, Dejana and Joksimović Jović, Jovana and Jakovljević, Vladimir and Živković, Vladimir and Nikolić, Maja and Sretenović, Jasmina and Nikolić, Marina and Jović, Nikola and Bićanin Ilić, Marija and Arsenijević, Petar and Dimitrijević, Aleksandra and Vulović, Tatjana and Ristić, Nataša and Bulatović, Kristina and Bolevich, Sergej and Stijak, Lazar and Pantović, Suzana",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.",
publisher = "Basel: MDPI",
journal = "Medicina",
title = "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats",
number = "6",
volume = "59",
doi = "10.3390/medicina59061104",
pages = "1104"
}

Rakić, D., Joksimović Jović, J., Jakovljević, V., Živković, V., Nikolić, M., Sretenović, J., Nikolić, M., Jović, N., Bićanin Ilić, M., Arsenijević, P., Dimitrijević, A., Vulović, T., Ristić, N., Bulatović, K., Bolevich, S., Stijak, L.,& Pantović, S.. (2023). High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina
Basel: MDPI., 59(6), 1104.
https://doi.org/10.3390/medicina59061104

Rakić D, Joksimović Jović J, Jakovljević V, Živković V, Nikolić M, Sretenović J, Nikolić M, Jović N, Bićanin Ilić M, Arsenijević P, Dimitrijević A, Vulović T, Ristić N, Bulatović K, Bolevich S, Stijak L, Pantović S. High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina. 2023;59(6):1104.
doi:10.3390/medicina59061104 .

Rakić, Dejana, Joksimović Jović, Jovana, Jakovljević, Vladimir, Živković, Vladimir, Nikolić, Maja, Sretenović, Jasmina, Nikolić, Marina, Jović, Nikola, Bićanin Ilić, Marija, Arsenijević, Petar, Dimitrijević, Aleksandra, Vulović, Tatjana, Ristić, Nataša, Bulatović, Kristina, Bolevich, Sergej, Stijak, Lazar, Pantović, Suzana, "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats" in Medicina, 59, no. 6 (2023):1104,
https://doi.org/10.3390/medicina59061104 . .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Joksimović Jović, Jovana
AU  - Srejović, Ivan
AU  - Živković, Vladimir
AU  - Mihajlović, Katarina
AU  - Labudović-Borović, Milica
AU  - Trifunović, Svetlana
AU  - Milošević, Verica
AU  - Lazić, Dejan
AU  - Bolevich, Sergey
AU  - Jakovljević, Vladimir
AU  - Milosavljević, Zoran
PY  - 2021
UR  - https://bacandrology.biomedcentral.com/articles/10.1186/s12610-021-00134-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4444
AB  - BACKGROUND During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction. RéSUMé: CONTEXTE: Au cours des dernières décennies, l’abus de stéroïdes androgéniques anabolisants (SAA) est devenu populaire parmi les athlètes professionnels et récréatifs. L’abus des SAA conduit à une diminution des niveaux d’hormones sexuelles, mais la littérature sur les effets de la natation seule ou combinée avec des SAA sur les tissus testiculaires est encore très limité. Le but de cette étude était d’étudier les effets de l’administration de quatre semaines de décanoate de nandrolone et de l’entraînement à la natation seuls ou en combinaison sur les paramètres morphométriques, le récepteur aux androgènes (RA) et l’état redox dans le tissu testiculaire. L’étude a inclus des rats mâles Wistar albinos, âgés de 10 semaines, classés en 4 groupes: contrôle (T-N-), nandrolone (T-N+), entraînement à la natation (T+N-) et entraînement à la natation avec nandrolone (T+N+). Les rats des groupes nandrolone (N+) ont reçu du décanoate de nandrolone 20 mg/kg p.c. une fois par semaine. Les rats des groupes entraînement (T+) nageaient 1 h/jour 5 jours/semaine. Les testicules isolés ont été mesurés, les testicules gauches ont été systématiquement traités pour l’analyse histologique tandis que les testicules droits ont été homogénéisés et préparés pour l’analyse des biomarqueurs de stress oxydatif suivants: indice de peroxydation lipidique (TBARS), nitrites, catalase, superoxyde dismutase (SOD) et glutathion réduit (GSH). RéSULTATS: Le diamètre, ainsi que la section transversale des tubules séminifères ont été réduits de 10 % et 21 % (respectivement) dans le groupe T-N+ et de 15 % et 41 % (respectivement) dans le groupe T+N+ par rapport au groupe témoin. L’interstitium des testicules était diminué dans tous les groupes expérimentaux. La réduction de l’immunoréactivité de RA dans le groupe T-N+ était de 22 %, dans le groupe T+N+ était de 9 % par rapport au groupe témoin. Les niveaux de TBARS ont augmenté dans les groupes T+N- et T+N+. Les nitrites ont diminué dans le groupe T+N+. L’activité de la catalase a été augmentée dans tous les groupes expérimentaux. La natation seule ou combinée à la nandrolone a réduit le niveau de GSH par rapport au contrôle. L’activité de la SOD était diminuée dans les groupes T-N+ et T+N+ par rapport au contrôle. CONCLUSIONS: La nandrolone seule ou combinée à la natation a diminué les paramètres morphométriques et la quantité de RA dans le tissu testiculaire. Les changements de l’état redox indiquent un dysfonctionnement de la reproduction.
PB  - London: BioMed Central Ltd
T2  - Basic and Clinical Andrology
T1  - Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats
IS  - 1
VL  - 31
DO  - 10.1186/s12610-021-00134-8
SP  - 17
ER  - 

@article{
author = "Sretenović, Jasmina and Joksimović Jović, Jovana and Srejović, Ivan and Živković, Vladimir and Mihajlović, Katarina and Labudović-Borović, Milica and Trifunović, Svetlana and Milošević, Verica and Lazić, Dejan and Bolevich, Sergey and Jakovljević, Vladimir and Milosavljević, Zoran",
year = "2021",
abstract = "BACKGROUND During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction. RéSUMé: CONTEXTE: Au cours des dernières décennies, l’abus de stéroïdes androgéniques anabolisants (SAA) est devenu populaire parmi les athlètes professionnels et récréatifs. L’abus des SAA conduit à une diminution des niveaux d’hormones sexuelles, mais la littérature sur les effets de la natation seule ou combinée avec des SAA sur les tissus testiculaires est encore très limité. Le but de cette étude était d’étudier les effets de l’administration de quatre semaines de décanoate de nandrolone et de l’entraînement à la natation seuls ou en combinaison sur les paramètres morphométriques, le récepteur aux androgènes (RA) et l’état redox dans le tissu testiculaire. L’étude a inclus des rats mâles Wistar albinos, âgés de 10 semaines, classés en 4 groupes: contrôle (T-N-), nandrolone (T-N+), entraînement à la natation (T+N-) et entraînement à la natation avec nandrolone (T+N+). Les rats des groupes nandrolone (N+) ont reçu du décanoate de nandrolone 20 mg/kg p.c. une fois par semaine. Les rats des groupes entraînement (T+) nageaient 1 h/jour 5 jours/semaine. Les testicules isolés ont été mesurés, les testicules gauches ont été systématiquement traités pour l’analyse histologique tandis que les testicules droits ont été homogénéisés et préparés pour l’analyse des biomarqueurs de stress oxydatif suivants: indice de peroxydation lipidique (TBARS), nitrites, catalase, superoxyde dismutase (SOD) et glutathion réduit (GSH). RéSULTATS: Le diamètre, ainsi que la section transversale des tubules séminifères ont été réduits de 10 % et 21 % (respectivement) dans le groupe T-N+ et de 15 % et 41 % (respectivement) dans le groupe T+N+ par rapport au groupe témoin. L’interstitium des testicules était diminué dans tous les groupes expérimentaux. La réduction de l’immunoréactivité de RA dans le groupe T-N+ était de 22 %, dans le groupe T+N+ était de 9 % par rapport au groupe témoin. Les niveaux de TBARS ont augmenté dans les groupes T+N- et T+N+. Les nitrites ont diminué dans le groupe T+N+. L’activité de la catalase a été augmentée dans tous les groupes expérimentaux. La natation seule ou combinée à la nandrolone a réduit le niveau de GSH par rapport au contrôle. L’activité de la SOD était diminuée dans les groupes T-N+ et T+N+ par rapport au contrôle. CONCLUSIONS: La nandrolone seule ou combinée à la natation a diminué les paramètres morphométriques et la quantité de RA dans le tissu testiculaire. Les changements de l’état redox indiquent un dysfonctionnement de la reproduction.",
publisher = "London: BioMed Central Ltd",
journal = "Basic and Clinical Andrology",
title = "Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats",
number = "1",
volume = "31",
doi = "10.1186/s12610-021-00134-8",
pages = "17"
}

Sretenović, J., Joksimović Jović, J., Srejović, I., Živković, V., Mihajlović, K., Labudović-Borović, M., Trifunović, S., Milošević, V., Lazić, D., Bolevich, S., Jakovljević, V.,& Milosavljević, Z.. (2021). Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats. in Basic and Clinical Andrology
London: BioMed Central Ltd., 31(1), 17.
https://doi.org/10.1186/s12610-021-00134-8

Sretenović J, Joksimović Jović J, Srejović I, Živković V, Mihajlović K, Labudović-Borović M, Trifunović S, Milošević V, Lazić D, Bolevich S, Jakovljević V, Milosavljević Z. Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats. in Basic and Clinical Andrology. 2021;31(1):17.
doi:10.1186/s12610-021-00134-8 .

Sretenović, Jasmina, Joksimović Jović, Jovana, Srejović, Ivan, Živković, Vladimir, Mihajlović, Katarina, Labudović-Borović, Milica, Trifunović, Svetlana, Milošević, Verica, Lazić, Dejan, Bolevich, Sergey, Jakovljević, Vladimir, Milosavljević, Zoran, "Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats" in Basic and Clinical Andrology, 31, no. 1 (2021):17,
https://doi.org/10.1186/s12610-021-00134-8 . .

TY  - JOUR
AU  - Joksimović Jović, Jovana
AU  - Jović, Nikola
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Rudić, Jovan
AU  - Milošević, Verica
AU  - Ristić, Nataša
AU  - Andrić, Kristina
AU  - Dimkić Tomić, Tijana
AU  - Miličić, Biljana
AU  - Jakovljević, Vladimir
PY  - 2021
UR  - https://rep.bioscientifica.com/view/journals/rep/163/1/REP-21-0219.xml
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4771
AB  - Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.
PB  - Society For Reproduction And Fertility
T2  - Reproduction (Cambridge, England)
T1  - Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.
IS  - 1
VL  - 163
DO  - 10.1530/REP-21-0219
SP  - 11
EP  - 21
ER  - 

@article{
author = "Joksimović Jović, Jovana and Jović, Nikola and Sretenović, Jasmina and Živković, Vladimir and Nikolić, Maja and Rudić, Jovan and Milošević, Verica and Ristić, Nataša and Andrić, Kristina and Dimkić Tomić, Tijana and Miličić, Biljana and Jakovljević, Vladimir",
year = "2021",
abstract = "Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.",
publisher = "Society For Reproduction And Fertility",
journal = "Reproduction (Cambridge, England)",
title = "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.",
number = "1",
volume = "163",
doi = "10.1530/REP-21-0219",
pages = "11-21"
}

Joksimović Jović, J., Jović, N., Sretenović, J., Živković, V., Nikolić, M., Rudić, J., Milošević, V., Ristić, N., Andrić, K., Dimkić Tomić, T., Miličić, B.,& Jakovljević, V.. (2021). Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England)
Society For Reproduction And Fertility., 163(1), 11-21.
https://doi.org/10.1530/REP-21-0219

Joksimović Jović J, Jović N, Sretenović J, Živković V, Nikolić M, Rudić J, Milošević V, Ristić N, Andrić K, Dimkić Tomić T, Miličić B, Jakovljević V. Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England). 2021;163(1):11-21.
doi:10.1530/REP-21-0219 .

Joksimović Jović, Jovana, Jović, Nikola, Sretenović, Jasmina, Živković, Vladimir, Nikolić, Maja, Rudić, Jovan, Milošević, Verica, Ristić, Nataša, Andrić, Kristina, Dimkić Tomić, Tijana, Miličić, Biljana, Jakovljević, Vladimir, "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress." in Reproduction (Cambridge, England), 163, no. 1 (2021):11-21,
https://doi.org/10.1530/REP-21-0219 . .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Trifunović, Miroljub
AU  - Pantović, Suzana
AU  - Jović, Slavoljub
AU  - Jakovljević, Vladimir
AU  - Bolevich, Sergey
AU  - Milosavljević, Zoran
AU  - Milošević, Verica
PY  - 2020
UR  - /core/journals/microscopy-and-microanalysis/article/immunohistomorphometric-and-hormonal-analysis-of-the-pituitary-gonadotropic-cells-after-application-of-the-nandrolone-decanoate-and-swimming-training-in-adult-male-rats/CB060C51EF744799F8CC8590E060CAB8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3717
AB  - The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.
PB  - Cambridge University Press (CUP)
T2  - Microscopy and Microanalysis
T1  - Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats
IS  - 4
VL  - 26
DO  - 10.1017/s143192762000166x
SP  - 699
EP  - 707
ER  - 

@article{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Ristić, Nataša and Trifunović, Miroljub and Pantović, Suzana and Jović, Slavoljub and Jakovljević, Vladimir and Bolevich, Sergey and Milosavljević, Zoran and Milošević, Verica",
year = "2020",
abstract = "The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.",
publisher = "Cambridge University Press (CUP)",
journal = "Microscopy and Microanalysis",
title = "Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats",
number = "4",
volume = "26",
doi = "10.1017/s143192762000166x",
pages = "699-707"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Ristić, N., Trifunović, M., Pantović, S., Jović, S., Jakovljević, V., Bolevich, S., Milosavljević, Z.,& Milošević, V.. (2020). Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats. in Microscopy and Microanalysis
Cambridge University Press (CUP)., 26(4), 699-707.
https://doi.org/10.1017/s143192762000166x

Sretenović J, Živković V, Srejović I, Ajdžanović V, Ristić N, Trifunović M, Pantović S, Jović S, Jakovljević V, Bolevich S, Milosavljević Z, Milošević V. Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats. in Microscopy and Microanalysis. 2020;26(4):699-707.
doi:10.1017/s143192762000166x .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Ristić, Nataša, Trifunović, Miroljub, Pantović, Suzana, Jović, Slavoljub, Jakovljević, Vladimir, Bolevich, Sergey, Milosavljević, Zoran, Milošević, Verica, "Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats" in Microscopy and Microanalysis, 26, no. 4 (2020):699-707,
https://doi.org/10.1017/s143192762000166x . .

TY  - CONF
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
AU  - Milosavljević, Zoran
AU  - Jakovljević, Vladimir
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6274
AB  - The study included 32 Wistar albino male rats, 10 weeks old, divided into 4 groups: control (T-N-), nandrolone (T-N+), swimming (T+N-) and swimming plus nandrolone (T+N+) groups. The T+ positive groups swam for 4 weeks, 1 hour/day, 5 days/week. The N+ positive groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Five micrometer thick sections were immunohistochemically stained, while LH and FSH cells were exposed to the morphometric analysis. Circulating level of LH, FSH and testosterone were measured. Body weight did not significantly decrease in all experimental groups compared to control. Absolute pituitary weights were decreased in T-N+ and increased in T+ positive groups. Relative pituitary weights were increased in all experimental groups compared to control. Volume density of LH cells was decreased for 48% in T-N+, for 22% in T+N- and for 35% in T+N+ group. Number of LH cells on mm2 was decreased for 36% in T-N+, for 3% in T+N- and for 27% in T+N+ group. Volume densities of FSH cells were decreased for 39% in T-N+, for 5.5% in T+N- and for 30% in T+N+ compared to control. Number of FSH cells was decreased for 13% in T-N+, for 1% and 15% in T+N+ group compared to control. Serum levels of LH were decreased in N+ positive groups, while FSH level was increased in T+N- group. Serum level of testosterone was increased in all experimental groups compared to control value. Nandrolone alone or combined with swimming decreased morphometric parameters of gonadotropic cells.
PB  - Belgrade: Serbian Physiology Society
C3  - Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia
T1  - Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6274
ER  - 

@conference{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Milošević, Verica and Milosavljević, Zoran and Jakovljević, Vladimir",
year = "2019",
abstract = "The study included 32 Wistar albino male rats, 10 weeks old, divided into 4 groups: control (T-N-), nandrolone (T-N+), swimming (T+N-) and swimming plus nandrolone (T+N+) groups. The T+ positive groups swam for 4 weeks, 1 hour/day, 5 days/week. The N+ positive groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Five micrometer thick sections were immunohistochemically stained, while LH and FSH cells were exposed to the morphometric analysis. Circulating level of LH, FSH and testosterone were measured. Body weight did not significantly decrease in all experimental groups compared to control. Absolute pituitary weights were decreased in T-N+ and increased in T+ positive groups. Relative pituitary weights were increased in all experimental groups compared to control. Volume density of LH cells was decreased for 48% in T-N+, for 22% in T+N- and for 35% in T+N+ group. Number of LH cells on mm2 was decreased for 36% in T-N+, for 3% in T+N- and for 27% in T+N+ group. Volume densities of FSH cells were decreased for 39% in T-N+, for 5.5% in T+N- and for 30% in T+N+ compared to control. Number of FSH cells was decreased for 13% in T-N+, for 1% and 15% in T+N+ group compared to control. Serum levels of LH were decreased in N+ positive groups, while FSH level was increased in T+N- group. Serum level of testosterone was increased in all experimental groups compared to control value. Nandrolone alone or combined with swimming decreased morphometric parameters of gonadotropic cells.",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia",
title = "Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6274"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Milošević, V., Milosavljević, Z.,& Jakovljević, V.. (2019). Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats. in Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia
Belgrade: Serbian Physiology Society., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_6274

Sretenović J, Živković V, Srejović I, Ajdžanović V, Milošević V, Milosavljević Z, Jakovljević V. Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats. in Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia. 2019;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_6274 .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Milošević, Verica, Milosavljević, Zoran, Jakovljević, Vladimir, "Morphometric analysis of pituitary gonadotropic cells after administration of nandrolone decanoate and swimming in adult rats" in Final program and abstract book: Third Joint Meeting of National Physiological Societies: "Health Risk, Nutrition and Dietary Supplements: Oxidative Stress and Polyphenols in the Heart of Serbian Wineries"; 2019 Jun 20-22; Topola, Serbia (2019):32,
https://hdl.handle.net/21.15107/rcub_ibiss_6274 .

TY  - CONF
AU  - Sretenović, Jasmina
AU  - Jakovljević, Biljana
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
AU  - Jakovljević, Vladimir
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6058
AB  - The aim of this study was to show the effects of moderate-intensity and high-intensity training on morfometric parameters of the left ventricle miocardium in normotensive and hypertensive rats. The study included 32-male Wistar albino and spontaneously hypertensive rats, divided into 6 groups: control normotensive (CTRL), normotensive moderate-intensity training (MIT), normotensive high-intensity training (HIIT), control hypertensive (SHR), hypertensive moderate-The rat ton Mist Roups and by peaterive high since sit training (Si HI days/week, Ih/day, while HIIT groups ran on speed from 35 to 55m/min, 30s in 5 cycles, with a 3 min period of rest between the cycles. After 4-weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin. Five micrometer thick sections were H&E stained. Captured microscopic images were processed by special software for image analysis to quantify the results. Body weights were decreased in all groups of hypertensive rats compared to normotensive rats. Relative heart weights were increased in hypertensive rats compared to normotensive rats. Longitudinal section diameter of cardiac muscle cells was decreased in SHR-MIT group for 13% compared to MIT, while in SHR-HIIT group the same parameter decreased for 17% compared to HIIT. Cross section muscle cell area was decreased: in SHR fl 7% compared to CTRL, in SHR-MIT for 6% compared to MIT, and in SHR-HIIT for 9% in comparison with HIIT. Both types of training caused significant hypertrophy of the left ventricle myocardium in normotensive and hypertensive rats
PB  - Kragujevac: University of Kragujevac, Faculty of Medical Sciences
C3  - Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia
T1  - Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6058
ER  - 

@conference{
author = "Sretenović, Jasmina and Jakovljević, Biljana and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Milošević, Verica and Jakovljević, Vladimir",
year = "2018",
abstract = "The aim of this study was to show the effects of moderate-intensity and high-intensity training on morfometric parameters of the left ventricle miocardium in normotensive and hypertensive rats. The study included 32-male Wistar albino and spontaneously hypertensive rats, divided into 6 groups: control normotensive (CTRL), normotensive moderate-intensity training (MIT), normotensive high-intensity training (HIIT), control hypertensive (SHR), hypertensive moderate-The rat ton Mist Roups and by peaterive high since sit training (Si HI days/week, Ih/day, while HIIT groups ran on speed from 35 to 55m/min, 30s in 5 cycles, with a 3 min period of rest between the cycles. After 4-weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin. Five micrometer thick sections were H&E stained. Captured microscopic images were processed by special software for image analysis to quantify the results. Body weights were decreased in all groups of hypertensive rats compared to normotensive rats. Relative heart weights were increased in hypertensive rats compared to normotensive rats. Longitudinal section diameter of cardiac muscle cells was decreased in SHR-MIT group for 13% compared to MIT, while in SHR-HIIT group the same parameter decreased for 17% compared to HIIT. Cross section muscle cell area was decreased: in SHR fl 7% compared to CTRL, in SHR-MIT for 6% compared to MIT, and in SHR-HIIT for 9% in comparison with HIIT. Both types of training caused significant hypertrophy of the left ventricle myocardium in normotensive and hypertensive rats",
publisher = "Kragujevac: University of Kragujevac, Faculty of Medical Sciences",
journal = "Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia",
title = "Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6058"
}

Sretenović, J., Jakovljević, B., Živković, V., Srejović, I., Ajdžanović, V., Milošević, V.,& Jakovljević, V.. (2018). Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats. in Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia
Kragujevac: University of Kragujevac, Faculty of Medical Sciences., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_6058

Sretenović J, Jakovljević B, Živković V, Srejović I, Ajdžanović V, Milošević V, Jakovljević V. Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats. in Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia. 2018;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_6058 .

Sretenović, Jasmina, Jakovljević, Biljana, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Milošević, Verica, Jakovljević, Vladimir, "Effects of two different types of training on morphometric parameters of the left ventricle miocardium in normotensive and hipertensive rats" in Final program and abstract book: 8th International Congress of Pathophysiology, Satelite symposium: Oxidative Stress in Health and Disease: from Basic Science to Applied Investigations; 2018 Sep 03; Kragujevac, Serbia (2018):32,
https://hdl.handle.net/21.15107/rcub_ibiss_6058 .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Jakovljević, Vladimir
AU  - Miler, Marko
AU  - Ristić, Nataša
AU  - Potrebić, Milica
AU  - Sretenović, Jasmina
AU  - Popovski Perčinić, Florina
AU  - Pendoski, Laze
AU  - Milošević, Verica
PY  - 2018
UR  - http://physiology.org.rs/4thCPS.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6035
AB  - The aim of this study was to investigate the immunohistomorphometric parameters of follicle-stimulating hormone (TSH) and luteinizing hormone (I.H) cells after testosterone application in an animal model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (Or) and testosterone treated orchidectomized (OrxT) groups. Testosterone propionate (5 mg/kg b.m. (day) was administered for three weeks. while Or group received the vehicle alone.
Immunohistochemically stained FSH and I cells were subjected to the morphometric and optical density-related analysis. while circulating concentration of testosterone was measured by the immunoassay. Serum concentration of testosterone was increased (ps0.05) by 24 fold in comparison with Orx group.
Testosterone treatment did not cause significant changes of body mass or absolute and relative pituitary weights compared to Or group. The volume of FSH and LH cells was decreased (ps0.05) by 51.3% and 56.6% respectively, in comparison with Or rats. Relative volume density of FSI and LE cells was also decreased (p<0.05) by 54.0% and 72.8% respectively, compared to Or group. Results related to the optical density of gonadotropic cells were in line with the morphometric findings i.e. this parameter of FSH and LH cells was decreased (P«0.05) by 25.7% and 16.2% respectively, in comparison with Or rats. In conclusion. applied testosterone decreased morphometric parameters and optical density of gonadotropic cells in Or rats.
PB  - Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)
C3  - Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
T1  - Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause
SP  - 118
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6035
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Jakovljević, Vladimir and Miler, Marko and Ristić, Nataša and Potrebić, Milica and Sretenović, Jasmina and Popovski Perčinić, Florina and Pendoski, Laze and Milošević, Verica",
year = "2018",
abstract = "The aim of this study was to investigate the immunohistomorphometric parameters of follicle-stimulating hormone (TSH) and luteinizing hormone (I.H) cells after testosterone application in an animal model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (Or) and testosterone treated orchidectomized (OrxT) groups. Testosterone propionate (5 mg/kg b.m. (day) was administered for three weeks. while Or group received the vehicle alone.
Immunohistochemically stained FSH and I cells were subjected to the morphometric and optical density-related analysis. while circulating concentration of testosterone was measured by the immunoassay. Serum concentration of testosterone was increased (ps0.05) by 24 fold in comparison with Orx group.
Testosterone treatment did not cause significant changes of body mass or absolute and relative pituitary weights compared to Or group. The volume of FSH and LH cells was decreased (ps0.05) by 51.3% and 56.6% respectively, in comparison with Or rats. Relative volume density of FSI and LE cells was also decreased (p<0.05) by 54.0% and 72.8% respectively, compared to Or group. Results related to the optical density of gonadotropic cells were in line with the morphometric findings i.e. this parameter of FSH and LH cells was decreased (P«0.05) by 25.7% and 16.2% respectively, in comparison with Or rats. In conclusion. applied testosterone decreased morphometric parameters and optical density of gonadotropic cells in Or rats.",
publisher = "Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)",
journal = "Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia",
title = "Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6035"
}

Ajdžanović, V., Jakovljević, V., Miler, M., Ristić, N., Potrebić, M., Sretenović, J., Popovski Perčinić, F., Pendoski, L.,& Milošević, V.. (2018). Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)., 118-118.
https://hdl.handle.net/21.15107/rcub_ibiss_6035

Ajdžanović V, Jakovljević V, Miler M, Ristić N, Potrebić M, Sretenović J, Popovski Perčinić F, Pendoski L, Milošević V. Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. 2018;:118-118.
https://hdl.handle.net/21.15107/rcub_ibiss_6035 .

Ajdžanović, Vladimir, Jakovljević, Vladimir, Miler, Marko, Ristić, Nataša, Potrebić, Milica, Sretenović, Jasmina, Popovski Perčinić, Florina, Pendoski, Laze, Milošević, Verica, "Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause" in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia (2018):118-118,
https://hdl.handle.net/21.15107/rcub_ibiss_6035 .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Popovska-Perčinić, Florina
AU  - Stojanović, Slobodan
AU  - Potrebić, Milica
AU  - Sretenović, Jasmina
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5992
AB  - Advanced age is frequently entwined with the adrenal cortex function impairment, glucocorticoid excess and following metabolic syndrome.
Also, age-dependent
disturbancies in aldosterone secretion and Na*/K homeostasis at least partly underlie the hypertension issues. Classical therapeutic approach in the treatment of some menopausal symptoms implies administration of estradiol or calcium, or their combination. Herein, we aimed to investigate the effects of chronic treatment with estradiol and calcium on some adrenal gland parameters in the ageing female rat model. Middle-aged (14-month-old) female Wistar rats were treated with estradiol dipropionate (EDP; 0.625 mg/kg b.m.) and calcium glucoheptonate (Ca; 11.4 mg/kg b.m.) daily for two weeks, while control ruts received vehicle alone by the same schedule. Combined treatment caused the decrease (p<0.05) of body mass by 21.5%, while the absolute and relative adrenal gland weights were increased (p<0.05) by16.2% and 43.8% respectively, compared to the adequate control values. The absolute adrenal gland volume, after treatment with EDP and Ca in the combination, was also increased (12.1%; p<0.05), in comparison with control group.
In parallel with that, circulating aldosterone was elevated (p<0.05) by 50.0%, compared to the control levels. However, combined treatment with EDP and Ca insignificantly (P>0.05) affected Na* and K' blood concentrations. It can be concluded that simultaneous estradiol ants calcium administration to middle-aged female rats enlarges the adrenal glands and have stimulatory influence on the mineralocorticoid output, which may represent unwanted effect from the perspective of blood pressure regulation.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5992
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Popovska-Perčinić, Florina and Stojanović, Slobodan and Potrebić, Milica and Sretenović, Jasmina and Jakovljević, Vladimir and Milošević, Verica",
year = "2018",
abstract = "Advanced age is frequently entwined with the adrenal cortex function impairment, glucocorticoid excess and following metabolic syndrome.
Also, age-dependent
disturbancies in aldosterone secretion and Na*/K homeostasis at least partly underlie the hypertension issues. Classical therapeutic approach in the treatment of some menopausal symptoms implies administration of estradiol or calcium, or their combination. Herein, we aimed to investigate the effects of chronic treatment with estradiol and calcium on some adrenal gland parameters in the ageing female rat model. Middle-aged (14-month-old) female Wistar rats were treated with estradiol dipropionate (EDP; 0.625 mg/kg b.m.) and calcium glucoheptonate (Ca; 11.4 mg/kg b.m.) daily for two weeks, while control ruts received vehicle alone by the same schedule. Combined treatment caused the decrease (p<0.05) of body mass by 21.5%, while the absolute and relative adrenal gland weights were increased (p<0.05) by16.2% and 43.8% respectively, compared to the adequate control values. The absolute adrenal gland volume, after treatment with EDP and Ca in the combination, was also increased (12.1%; p<0.05), in comparison with control group.
In parallel with that, circulating aldosterone was elevated (p<0.05) by 50.0%, compared to the control levels. However, combined treatment with EDP and Ca insignificantly (P>0.05) affected Na* and K' blood concentrations. It can be concluded that simultaneous estradiol ants calcium administration to middle-aged female rats enlarges the adrenal glands and have stimulatory influence on the mineralocorticoid output, which may represent unwanted effect from the perspective of blood pressure regulation.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats",
pages = "149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5992"
}

Ajdžanović, V., Jarić, I., Popovska-Perčinić, F., Stojanović, S., Potrebić, M., Sretenović, J., Jakovljević, V.,& Milošević, V.. (2018). Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 149.
https://hdl.handle.net/21.15107/rcub_ibiss_5992

Ajdžanović V, Jarić I, Popovska-Perčinić F, Stojanović S, Potrebić M, Sretenović J, Jakovljević V, Milošević V. Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:149.
https://hdl.handle.net/21.15107/rcub_ibiss_5992 .

Ajdžanović, Vladimir, Jarić, Ivana, Popovska-Perčinić, Florina, Stojanović, Slobodan, Potrebić, Milica, Sretenović, Jasmina, Jakovljević, Vladimir, Milošević, Verica, "Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):149,
https://hdl.handle.net/21.15107/rcub_ibiss_5992 .

TY  - CONF
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
AU  - Jakovljević, Vladimir
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5993
AB  - Anabolic androgenic steroids (ASs) are synthetic analogs of testosterone. Because of its anabolic effect, in recent decades, AAS became very popular among professional and recreational sports man. The aim of this study was to examine the chronic effects of exercise training alone or combined with supraphysiological doses of nandrolone decanoate (ND) on cardiac muscle of the left ventricle. The study included 32 Wistar albino male rats, divided into 4 groups: control (I-N-), nandrolone (I-N+), exercise training (T+N-) and exercise training plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 3 weeks, 1 hour/day, 5 days/week. The T-N+ and T+N+ groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. After 3 weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin.
Five micrometer thick sections were stained with hematoxylin and eosin (H/E). Captured microscopic images were processed by special software for image analysis to quantify results. Our result show that nandrolone alone causes left ventricular wall thickening for 4% (p > 0.05), training alone 14% (p > 0.05) while training combined with nandrolone leads to increased wall thickening for 24% (p < 0.05). Longitudinal section diameter of cardiac muscle cells was increased for 6% (p >0.05), in T-N+ for 23% (p < 0.05), in T*N- and for 33% (p < 0.05) in T+N+ group. Comparison between the experimental groups showed that the largest difference of longitudinal section diameter can be observed between T-N+ and T+N+ (24%; p < 0.05) and the lowest difference was found between T+N- and T#N+ (8%; p > 0.05). Cross section muscle cell area was increased in T-N+ for 7% (p > 0.05), in T+N- for 29% (p < 0.05), in T+N+ for 31% (p < 0.05). Comparison between the experimental groups showed that the difference of cross section area between T-N+ and T+N+ was 22% (p < 0.05) and the lowest, non-significant difference was found between T+N- and T+N+ (1.6%; p > 0.05). Chronic administration of supraphysiological doses of ND alone promote mild cardiac muscle hypertrophy, but only in combination with the exercise training the degree of the cardiac muscle hypertrophy of the left ventricle is significant.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5993
ER  - 

@conference{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Milošević, Verica and Jakovljević, Vladimir",
year = "2018",
abstract = "Anabolic androgenic steroids (ASs) are synthetic analogs of testosterone. Because of its anabolic effect, in recent decades, AAS became very popular among professional and recreational sports man. The aim of this study was to examine the chronic effects of exercise training alone or combined with supraphysiological doses of nandrolone decanoate (ND) on cardiac muscle of the left ventricle. The study included 32 Wistar albino male rats, divided into 4 groups: control (I-N-), nandrolone (I-N+), exercise training (T+N-) and exercise training plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 3 weeks, 1 hour/day, 5 days/week. The T-N+ and T+N+ groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. After 3 weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin.
Five micrometer thick sections were stained with hematoxylin and eosin (H/E). Captured microscopic images were processed by special software for image analysis to quantify results. Our result show that nandrolone alone causes left ventricular wall thickening for 4% (p > 0.05), training alone 14% (p > 0.05) while training combined with nandrolone leads to increased wall thickening for 24% (p < 0.05). Longitudinal section diameter of cardiac muscle cells was increased for 6% (p >0.05), in T-N+ for 23% (p < 0.05), in T*N- and for 33% (p < 0.05) in T+N+ group. Comparison between the experimental groups showed that the largest difference of longitudinal section diameter can be observed between T-N+ and T+N+ (24%; p < 0.05) and the lowest difference was found between T+N- and T#N+ (8%; p > 0.05). Cross section muscle cell area was increased in T-N+ for 7% (p > 0.05), in T+N- for 29% (p < 0.05), in T+N+ for 31% (p < 0.05). Comparison between the experimental groups showed that the difference of cross section area between T-N+ and T+N+ was 22% (p < 0.05) and the lowest, non-significant difference was found between T+N- and T+N+ (1.6%; p > 0.05). Chronic administration of supraphysiological doses of ND alone promote mild cardiac muscle hypertrophy, but only in combination with the exercise training the degree of the cardiac muscle hypertrophy of the left ventricle is significant.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats",
pages = "150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5993"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Milošević, V.,& Jakovljević, V.. (2018). Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 150.
https://hdl.handle.net/21.15107/rcub_ibiss_5993

Sretenović J, Živković V, Srejović I, Ajdžanović V, Milošević V, Jakovljević V. Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:150.
https://hdl.handle.net/21.15107/rcub_ibiss_5993 .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Milošević, Verica, Jakovljević, Vladimir, "Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):150,
https://hdl.handle.net/21.15107/rcub_ibiss_5993 .

TY  - CONF
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
AU  - Pendovski, Lazo
AU  - Potrebić, Milica
AU  - Ušćebrka, Gordana
AU  - Sretenović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
PY  - 2018
UR  - http://www.enea2018.com/program.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5925
PB  - European NeuroEndocrine Association
C3  - 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
T1  - The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5925
ER  - 

@conference{
author = "Popovska-Perčinić, Florina and Milošević, Verica and Pendovski, Lazo and Potrebić, Milica and Ušćebrka, Gordana and Sretenović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir",
year = "2018",
publisher = "European NeuroEndocrine Association",
journal = "18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland",
title = "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5925"
}

Popovska-Perčinić, F., Milošević, V., Pendovski, L., Potrebić, M., Ušćebrka, G., Sretenović, J., Jarić, I.,& Ajdžanović, V.. (2018). The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
European NeuroEndocrine Association., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925

Popovska-Perčinić F, Milošević V, Pendovski L, Potrebić M, Ušćebrka G, Sretenović J, Jarić I, Ajdžanović V. The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland. 2018;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

Popovska-Perčinić, Florina, Milošević, Verica, Pendovski, Lazo, Potrebić, Milica, Ušćebrka, Gordana, Sretenović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study" in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland (2018):8,
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Čorbić, Milena
AU  - Milošević, Verica
AU  - Jakovljević, Vladimir
AU  - Milosavljević, Zoran
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0065128118300382?via%3Dihub
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29759662
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3059
AB  - Anabolic androgenic steroids (AASs) are synthetic analogs of testosterone often used by athletes to increase the skeletal muscle mass. Our goal was to examine the effects of physical activity and physical activity combined with supraphysiological doses of nandrolone on functional morphology of the quadriceps muscle. The study included 32 peripubertal Wistar rats, divided into 4 groups: control (T-N-), nandrolone (T-N+), physical activity (T+N-) and physical activity plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 4 weeks, 1 h/day, 5 days/week. The T-N+ and T+N+ groups received nandolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Subsequently, the rats were sacrificed and muscle specimens were prepared for the processing. Tissue sections were histochemically and immunohistochemically stained, while the image analysis was used for quantification. Longitudinal diameter of quadriceps muscle cells was increased for 21% in T-N+, for 57% in T+N- and for 64% in T+N+ group while cross section muscle cell area was increased in T-N+ for 19%, in T+N- for 47% and in T+N+ group for 59%, compared to the control. Collagen fibers covered area was increased in T-N+ group for 36%, in T+N- for 109% and in T+N+ group for 159%, compared to the control. Erythrocyte depots were decreased in T-N+ group and increased in T+N- and T+N+ group, in comparison with T-N-. VEGF depots were increased in all treated groups. Chronic administration of supraphysiological doses of AASs alone or in combination with physical activity induces hypertrophy and significant changes in the quadriceps muscle tissue structure.
T2  - Acta Histochemica
T1  - Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.
DO  - 10.1016/j.acthis.2018.04.004
ER  - 

@article{
author = "Sretenović, Jasmina and Ajdžanović, Vladimir and Živković, Vladimir and Srejović, Ivan and Čorbić, Milena and Milošević, Verica and Jakovljević, Vladimir and Milosavljević, Zoran",
year = "2018",
abstract = "Anabolic androgenic steroids (AASs) are synthetic analogs of testosterone often used by athletes to increase the skeletal muscle mass. Our goal was to examine the effects of physical activity and physical activity combined with supraphysiological doses of nandrolone on functional morphology of the quadriceps muscle. The study included 32 peripubertal Wistar rats, divided into 4 groups: control (T-N-), nandrolone (T-N+), physical activity (T+N-) and physical activity plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 4 weeks, 1 h/day, 5 days/week. The T-N+ and T+N+ groups received nandolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Subsequently, the rats were sacrificed and muscle specimens were prepared for the processing. Tissue sections were histochemically and immunohistochemically stained, while the image analysis was used for quantification. Longitudinal diameter of quadriceps muscle cells was increased for 21% in T-N+, for 57% in T+N- and for 64% in T+N+ group while cross section muscle cell area was increased in T-N+ for 19%, in T+N- for 47% and in T+N+ group for 59%, compared to the control. Collagen fibers covered area was increased in T-N+ group for 36%, in T+N- for 109% and in T+N+ group for 159%, compared to the control. Erythrocyte depots were decreased in T-N+ group and increased in T+N- and T+N+ group, in comparison with T-N-. VEGF depots were increased in all treated groups. Chronic administration of supraphysiological doses of AASs alone or in combination with physical activity induces hypertrophy and significant changes in the quadriceps muscle tissue structure.",
journal = "Acta Histochemica",
title = "Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.",
doi = "10.1016/j.acthis.2018.04.004"
}

Sretenović, J., Ajdžanović, V., Živković, V., Srejović, I., Čorbić, M., Milošević, V., Jakovljević, V.,& Milosavljević, Z.. (2018). Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.. in Acta Histochemica.
https://doi.org/10.1016/j.acthis.2018.04.004

Sretenović J, Ajdžanović V, Živković V, Srejović I, Čorbić M, Milošević V, Jakovljević V, Milosavljević Z. Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.. in Acta Histochemica. 2018;.
doi:10.1016/j.acthis.2018.04.004 .

Sretenović, Jasmina, Ajdžanović, Vladimir, Živković, Vladimir, Srejović, Ivan, Čorbić, Milena, Milošević, Verica, Jakovljević, Vladimir, Milosavljević, Zoran, "Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats." in Acta Histochemica (2018),
https://doi.org/10.1016/j.acthis.2018.04.004 . .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Čorbić, Milena
AU  - Milošević, Verica
AU  - Jakovljević, Vladimir
AU  - Milosavljević, Zoran
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0065128118300382?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3085
AB  - Anabolic androgenic steroids (AASs) are synthetic analogs of testosterone often used by athletes to increase the skeletal muscle mass. Our goal was to examine the effects of physical activity and physical activity combined with supraphysiological doses of nandrolone on functional morphology of the quadriceps muscle. The study included 32 peripubertal Wistar rats, divided into 4 groups: control (T-N-), nandrolone (T-N+), physical activity (T+N-) and physical activity plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 4 weeks, 1 h/day, 5 days/week. The T-N+ and T+N+ groups received nandolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Subsequently, the rats were sacrificed and muscle specimens were prepared for the processing. Tissue sections were histochemically and immunohistochemically stained, while the image analysis was used for quantification. Longitudinal diameter of quadriceps muscle cells was increased for 21% in T-N+, for 57% in T+N- and for 64% in T+N+ group while cross section muscle cell area was increased in T-N+ for 19%, in T+N- for 47% and in T+N+ group for 59%, compared to the control. Collagen fibers covered area was increased in T-N+ group for 36%, in T+N- for 109% and in T+N+ group for 159%, compared to the control. Erythrocyte depots were decreased in T-N+ group and increased in T+N- and T+N+ group, in comparison with T-N-. VEGF depots were increased in all treated groups. Chronic administration of supraphysiological doses of AASs alone or in combination with physical activity induces hypertrophy and significant changes in the quadriceps muscle tissue structure.
T2  - Acta Histochemica
T1  - Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.
IS  - 5
VL  - 120
DO  - 10.1016/j.acthis.2018.04.004
SP  - 429
EP  - 437
ER  - 

@article{
author = "Sretenović, Jasmina and Ajdžanović, Vladimir and Živković, Vladimir and Srejović, Ivan and Čorbić, Milena and Milošević, Verica and Jakovljević, Vladimir and Milosavljević, Zoran",
year = "2018",
abstract = "Anabolic androgenic steroids (AASs) are synthetic analogs of testosterone often used by athletes to increase the skeletal muscle mass. Our goal was to examine the effects of physical activity and physical activity combined with supraphysiological doses of nandrolone on functional morphology of the quadriceps muscle. The study included 32 peripubertal Wistar rats, divided into 4 groups: control (T-N-), nandrolone (T-N+), physical activity (T+N-) and physical activity plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 4 weeks, 1 h/day, 5 days/week. The T-N+ and T+N+ groups received nandolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. Subsequently, the rats were sacrificed and muscle specimens were prepared for the processing. Tissue sections were histochemically and immunohistochemically stained, while the image analysis was used for quantification. Longitudinal diameter of quadriceps muscle cells was increased for 21% in T-N+, for 57% in T+N- and for 64% in T+N+ group while cross section muscle cell area was increased in T-N+ for 19%, in T+N- for 47% and in T+N+ group for 59%, compared to the control. Collagen fibers covered area was increased in T-N+ group for 36%, in T+N- for 109% and in T+N+ group for 159%, compared to the control. Erythrocyte depots were decreased in T-N+ group and increased in T+N- and T+N+ group, in comparison with T-N-. VEGF depots were increased in all treated groups. Chronic administration of supraphysiological doses of AASs alone or in combination with physical activity induces hypertrophy and significant changes in the quadriceps muscle tissue structure.",
journal = "Acta Histochemica",
title = "Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.",
number = "5",
volume = "120",
doi = "10.1016/j.acthis.2018.04.004",
pages = "429-437"
}

Sretenović, J., Ajdžanović, V., Živković, V., Srejović, I., Čorbić, M., Milošević, V., Jakovljević, V.,& Milosavljević, Z.. (2018). Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.. in Acta Histochemica, 120(5), 429-437.
https://doi.org/10.1016/j.acthis.2018.04.004

Sretenović J, Ajdžanović V, Živković V, Srejović I, Čorbić M, Milošević V, Jakovljević V, Milosavljević Z. Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats.. in Acta Histochemica. 2018;120(5):429-437.
doi:10.1016/j.acthis.2018.04.004 .

Sretenović, Jasmina, Ajdžanović, Vladimir, Živković, Vladimir, Srejović, Ivan, Čorbić, Milena, Milošević, Verica, Jakovljević, Vladimir, Milosavljević, Zoran, "Nandrolone decanoate and physical activity affect quadriceps in peripubertal rats." in Acta Histochemica, 120, no. 5 (2018):429-437,
https://doi.org/10.1016/j.acthis.2018.04.004 . .