TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan B
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Trifunović, Svetlana
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1306
AB  - A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.
PB  - Springer Nature
T2  - Journal of Membrane Biology
T1  - The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study
IS  - 3
VL  - 239
DO  - 10.1007/s00232-010-9332-8
SP  - 131
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1306
ER  - 

@article{
author = "Ajdžanović, Vladimir and Spasojević, Ivan B and Šošić-Jurjević, Branka  and Filipović, Branko and Trifunović, Svetlana and Sekulić, Milka I. and Milošević, Verica",
year = "2011",
abstract = "A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.",
publisher = "Springer Nature",
journal = "Journal of Membrane Biology",
title = "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study",
number = "3",
volume = "239",
doi = "10.1007/s00232-010-9332-8",
pages = "131-135",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1306"
}

Ajdžanović, V., Spasojević, I. B., Šošić-Jurjević, B., Filipović, B., Trifunović, S., Sekulić, M. I.,& Milošević, V.. (2011). The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology
Springer Nature., 239(3), 131-135.
https://doi.org/10.1007/s00232-010-9332-8
https://hdl.handle.net/21.15107/rcub_ibiss_1306

Ajdžanović V, Spasojević IB, Šošić-Jurjević B, Filipović B, Trifunović S, Sekulić MI, Milošević V. The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology. 2011;239(3):131-135.
doi:10.1007/s00232-010-9332-8
https://hdl.handle.net/21.15107/rcub_ibiss_1306 .

Ajdžanović, Vladimir, Spasojević, Ivan B, Šošić-Jurjević, Branka , Filipović, Branko, Trifunović, Svetlana, Sekulić, Milka I., Milošević, Verica, "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study" in Journal of Membrane Biology, 239, no. 3 (2011):131-135,
https://doi.org/10.1007/s00232-010-9332-8 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1306 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
AU  - Nestorović, Nataša
AU  - Filipović, Branko
AU  - Manojlović-Stojanoski, Milica
AU  - Živanović, Jasmina
AU  - Sekulić, Milka I.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1307
AB  - Administration of estradiol or calcium, or combined, represents the classical therapeutic approach in the treatment of some menopausal symptoms. We have studied the effects of estradiol dipropionate (EDP) and calcium glucoheptonate (Ca) on morphological and hormonal features of the adrenal gland in 14-month-old female Wistar rats. The animals were treated with EDP (0.625 mg/kg b.w.) or Ca (11.4 mg/kg b.w.) daily for two weeks, with control rats receiving vehicle alone by the same schedule. The cell volumes in the zona glomerulosa (ZG) and zona fasciculata (ZF) were 11.2% and 5.5% greater (P < 0.05) and in the zona reticularis (ZR) 13.0% smaller (P < 0.05) in the EDP group than in the control group. In the Ca group, cell volume in the ZG was increased by 5.6% (P < 0.05), while cell volumes in the ZF and ZR were decreased by 26.0% and 14.7%, respectively (P < 0.05), in comparison with control values. Serum aldosterone and corticosterone concentrations were higher in the EDP-treated (by 27.8% and 19.8%, respectively) and Ca-treated (by 80.0% and 24.1%, respectively) groups in comparison with the control group (P < 0.05). These data suggest that EDP and Ca treatments have stimulatory effects on the ZG and ZF, but inhibitory effects on the ZR in middle-aged female rats.
T2  - Central European Journal of Biology
T1  - The adrenal cortex in female rats after estradiol or calcium treatment
IS  - 1
VL  - 6
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1307
ER  - 

@article{
author = "Milošević, Verica and Ajdžanović, Vladimir and Nestorović, Nataša and Filipović, Branko and Manojlović-Stojanoski, Milica and Živanović, Jasmina and Sekulić, Milka I.",
year = "2011",
abstract = "Administration of estradiol or calcium, or combined, represents the classical therapeutic approach in the treatment of some menopausal symptoms. We have studied the effects of estradiol dipropionate (EDP) and calcium glucoheptonate (Ca) on morphological and hormonal features of the adrenal gland in 14-month-old female Wistar rats. The animals were treated with EDP (0.625 mg/kg b.w.) or Ca (11.4 mg/kg b.w.) daily for two weeks, with control rats receiving vehicle alone by the same schedule. The cell volumes in the zona glomerulosa (ZG) and zona fasciculata (ZF) were 11.2% and 5.5% greater (P < 0.05) and in the zona reticularis (ZR) 13.0% smaller (P < 0.05) in the EDP group than in the control group. In the Ca group, cell volume in the ZG was increased by 5.6% (P < 0.05), while cell volumes in the ZF and ZR were decreased by 26.0% and 14.7%, respectively (P < 0.05), in comparison with control values. Serum aldosterone and corticosterone concentrations were higher in the EDP-treated (by 27.8% and 19.8%, respectively) and Ca-treated (by 80.0% and 24.1%, respectively) groups in comparison with the control group (P < 0.05). These data suggest that EDP and Ca treatments have stimulatory effects on the ZG and ZF, but inhibitory effects on the ZR in middle-aged female rats.",
journal = "Central European Journal of Biology",
title = "The adrenal cortex in female rats after estradiol or calcium treatment",
number = "1",
volume = "6",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1307"
}

Milošević, V., Ajdžanović, V., Nestorović, N., Filipović, B., Manojlović-Stojanoski, M., Živanović, J.,& Sekulić, M. I.. (2011). The adrenal cortex in female rats after estradiol or calcium treatment. in Central European Journal of Biology, 6(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1307

Milošević V, Ajdžanović V, Nestorović N, Filipović B, Manojlović-Stojanoski M, Živanović J, Sekulić MI. The adrenal cortex in female rats after estradiol or calcium treatment. in Central European Journal of Biology. 2011;6(1):null-47.
https://hdl.handle.net/21.15107/rcub_ibiss_1307 .

Milošević, Verica, Ajdžanović, Vladimir, Nestorović, Nataša, Filipović, Branko, Manojlović-Stojanoski, Milica, Živanović, Jasmina, Sekulić, Milka I., "The adrenal cortex in female rats after estradiol or calcium treatment" in Central European Journal of Biology, 6, no. 1 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1307 .

TY  - CONF
AU  - Milošević, Verica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Marković, Z
AU  - Manojlović-Stojanoski, Milica
AU  - Jarić, Ivana
AU  - Brkić, Branislava
AU  - Sekulić, Milka I.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1293
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol
IS  - null
VL  - 49
EP  - S435
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1293
ER  - 

@conference{
author = "Milošević, Verica and Trifunović, Svetlana and Ajdžanović, Vladimir and Marković, Z and Manojlović-Stojanoski, Milica and Jarić, Ivana and Brkić, Branislava and Sekulić, Milka I.",
year = "2011",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol",
number = "null",
volume = "49",
pages = "S435",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1293"
}

Milošević, V., Trifunović, S., Ajdžanović, V., Marković, Z., Manojlović-Stojanoski, M., Jarić, I., Brkić, B.,& Sekulić, M. I.. (2011). Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol. in Clinical Chemistry and Laboratory Medicine, 49(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1293

Milošević V, Trifunović S, Ajdžanović V, Marković Z, Manojlović-Stojanoski M, Jarić I, Brkić B, Sekulić MI. Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol. in Clinical Chemistry and Laboratory Medicine. 2011;49(null):null-S435.
https://hdl.handle.net/21.15107/rcub_ibiss_1293 .

Milošević, Verica, Trifunović, Svetlana, Ajdžanović, Vladimir, Marković, Z, Manojlović-Stojanoski, Milica, Jarić, Ivana, Brkić, Branislava, Sekulić, Milka I., "Immunopositive Gh Cells in Long-Term Ovariectomized Female Rats Treated With Estradiol" in Clinical Chemistry and Laboratory Medicine, 49, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1293 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan B
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1389
AB  - The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p < 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p < 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.
PB  - Canadian Science Publishing; National Research Council of Canada
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study
IS  - 4
VL  - 88
DO  - 10.1139/Y10-020
SP  - 497
EP  - 500
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1389
ER  - 

@article{
author = "Ajdžanović, Vladimir and Spasojević, Ivan B and Filipović, Branko and Šošić-Jurjević, Branka  and Sekulić, Milka I. and Milošević, Verica",
year = "2010",
abstract = "The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p < 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p < 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.",
publisher = "Canadian Science Publishing; National Research Council of Canada",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study",
number = "4",
volume = "88",
doi = "10.1139/Y10-020",
pages = "497-500",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1389"
}

Ajdžanović, V., Spasojević, I. B., Filipović, B., Šošić-Jurjević, B., Sekulić, M. I.,& Milošević, V.. (2010). Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology
Canadian Science Publishing; National Research Council of Canada., 88(4), 497-500.
https://doi.org/10.1139/Y10-020
https://hdl.handle.net/21.15107/rcub_ibiss_1389

Ajdžanović V, Spasojević IB, Filipović B, Šošić-Jurjević B, Sekulić MI, Milošević V. Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology. 2010;88(4):497-500.
doi:10.1139/Y10-020
https://hdl.handle.net/21.15107/rcub_ibiss_1389 .

Ajdžanović, Vladimir, Spasojević, Ivan B, Filipović, Branko, Šošić-Jurjević, Branka , Sekulić, Milka I., Milošević, Verica, "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study" in Canadian Journal of Physiology and Pharmacology, 88, no. 4 (2010):497-500,
https://doi.org/10.1139/Y10-020 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1389 .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Savin, Svetlana B
AU  - Nestorović, Nataša
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1380
AB  - High intake of soybean phytoestrogens, isoflavones genistein (G) and daidzein (D), has been associated with health benefits. However, isoflavones were reported to affect adversely thyroid function in the presence of other goitrogenic factors. As the thyroid gland becomes functionally impaired with age, we examined whether supplementary doses of G or D would affect morphology and function of pituitary-thyroid axis in middle-aged male rats. Sixteen-month-old orchidectomized Wistar rats were treated with 10 mg/kg of either G or D, while the control sham-operated and orchidectomized group received just the vehicle for three weeks. The animals were fed soy-free diet with increased iodine content, and killed 24 h after the last treatment. Their pituitaries and thyroids were excised and prepared for further immunohistochemical and morphometric investigation. The concentrations of thyroid-stimulating hormone (TSH), total T(4) and T(3), in the serum were determined. In both isoflavone-treated groups, pituitary TSH-immunopositive cells had increased cellular volume and relative volume density (P < 0.05), as well as increased serum TSH levels (P < 0.05) in comparison to the controls; their thyroid tissue was characterized by increased volume of thyroglobulin-immunopositive epithelium (P < 0.05), epithelial height and index of activation rate (P < 0.05), while the volume of luminal colloid, and total serum T(4) and T(3) levels decreased (P < 0.05) in comparison to the controls. In conclusion, this study provides the first direct evidence that both G and D can induce microfollicular changes in the thyroid tissue and reduce the level of thyroid hormones in Orx middle-aged male rats, a model of andropause. This reduction consequently led to a feedback stimulation of pituitary TSH cells. The detected stimulatory effect was higher in the daidzein-treated rats.
PB  - Society for Experimental Biology and Medicine
T2  - Experimental Biology and Medicine
T1  - Suppressive effects of genistein and daidzein on pituitary-thyroid axis in orchidectomized middle-aged rats
IS  - 5
VL  - 235
DO  - 10.1258/ebm.2009.009279
SP  - 590
EP  - 598
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1380
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Filipović, Branko and Ajdžanović, Vladimir and Savin, Svetlana B and Nestorović, Nataša and Milošević, Verica and Sekulić, Milka I.",
year = "2010",
abstract = "High intake of soybean phytoestrogens, isoflavones genistein (G) and daidzein (D), has been associated with health benefits. However, isoflavones were reported to affect adversely thyroid function in the presence of other goitrogenic factors. As the thyroid gland becomes functionally impaired with age, we examined whether supplementary doses of G or D would affect morphology and function of pituitary-thyroid axis in middle-aged male rats. Sixteen-month-old orchidectomized Wistar rats were treated with 10 mg/kg of either G or D, while the control sham-operated and orchidectomized group received just the vehicle for three weeks. The animals were fed soy-free diet with increased iodine content, and killed 24 h after the last treatment. Their pituitaries and thyroids were excised and prepared for further immunohistochemical and morphometric investigation. The concentrations of thyroid-stimulating hormone (TSH), total T(4) and T(3), in the serum were determined. In both isoflavone-treated groups, pituitary TSH-immunopositive cells had increased cellular volume and relative volume density (P < 0.05), as well as increased serum TSH levels (P < 0.05) in comparison to the controls; their thyroid tissue was characterized by increased volume of thyroglobulin-immunopositive epithelium (P < 0.05), epithelial height and index of activation rate (P < 0.05), while the volume of luminal colloid, and total serum T(4) and T(3) levels decreased (P < 0.05) in comparison to the controls. In conclusion, this study provides the first direct evidence that both G and D can induce microfollicular changes in the thyroid tissue and reduce the level of thyroid hormones in Orx middle-aged male rats, a model of andropause. This reduction consequently led to a feedback stimulation of pituitary TSH cells. The detected stimulatory effect was higher in the daidzein-treated rats.",
publisher = "Society for Experimental Biology and Medicine",
journal = "Experimental Biology and Medicine",
title = "Suppressive effects of genistein and daidzein on pituitary-thyroid axis in orchidectomized middle-aged rats",
number = "5",
volume = "235",
doi = "10.1258/ebm.2009.009279",
pages = "590-598",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1380"
}

Šošić-Jurjević, B., Filipović, B., Ajdžanović, V., Savin, S. B., Nestorović, N., Milošević, V.,& Sekulić, M. I.. (2010). Suppressive effects of genistein and daidzein on pituitary-thyroid axis in orchidectomized middle-aged rats. in Experimental Biology and Medicine
Society for Experimental Biology and Medicine., 235(5), 590-598.
https://doi.org/10.1258/ebm.2009.009279
https://hdl.handle.net/21.15107/rcub_ibiss_1380

Šošić-Jurjević B, Filipović B, Ajdžanović V, Savin SB, Nestorović N, Milošević V, Sekulić MI. Suppressive effects of genistein and daidzein on pituitary-thyroid axis in orchidectomized middle-aged rats. in Experimental Biology and Medicine. 2010;235(5):590-598.
doi:10.1258/ebm.2009.009279
https://hdl.handle.net/21.15107/rcub_ibiss_1380 .

Šošić-Jurjević, Branka , Filipović, Branko, Ajdžanović, Vladimir, Savin, Svetlana B, Nestorović, Nataša, Milošević, Verica, Sekulić, Milka I., "Suppressive effects of genistein and daidzein on pituitary-thyroid axis in orchidectomized middle-aged rats" in Experimental Biology and Medicine, 235, no. 5 (2010):590-598,
https://doi.org/10.1258/ebm.2009.009279 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1380 .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Sekulić, Milka I.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1332
AB  - Glucocorticoids have an inhibitory influence on proliferation activity of the pituitary cells while stimulating apoptosis. Therefore, it was hypothesized that the synthetic glucocorticoid, dexamethasone (DX), has an inhibitory influence on the number of thyroid-stimulating hormone (TSH) cells during fetal development. The effects of maternal administration of DX on stereological parameters of TSH cells, and TSH serum concentration were investigated in 21-day-old rat fetuses. On day 16 of pregnancy, the experimental dams received 1.0 mg DX/kg b.w. subcutaneously, followed by 0.5 mg DX/kg b.w./day on days 17 and 18 of gestation. The control gravid females received the same volume of saline vehicle. TSH cells were stained immunocytochemically by the peroxidase-antiperoxidase (PAP) method. The fetal pituitary volumes were estimated using Cavalieri's principle. A physical disector counting technique in combination with the fractionator sampling method was used for estimation of pituitary TSH cell number. Cell and nuclear volumes were measured with a planar rotator. Maternal DX application was found to cause a significant decrease of pituitary volume and number of TSH cells per pituitary in 21-day-old fetuses in comparison with the control fetuses. TSH cell number expressed per body weight unit declined significantly after maternal DX administration. These results indicate an inhibitory DX influence on proliferative activity of precursors and likely differentiated TSH cells and increased apoptotic prevalence. The histological appearance, volume of TSH cells and TSH serum concentration suggest intensive synthetic activity in TSH cells of DX exposed fetuses. Microsc. Res. Tech. 73:1077-1085, 2010. (C) 2010 Wiley-Liss, Inc.
PB  - John Wiley and Sons
T2  - Microscopy Research and Technique
T1  - Unbiased Stereological Estimation of the Rat Fetal Pituitary Volume and of the Total Number and Volume of TSH Cells After Maternal Dexamethasone Application
IS  - 12
VL  - 73
DO  - 10.1002/jemt.20842
SP  - 1077
EP  - 1085
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1332
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Filipović, Branko and Šošić-Jurjević, Branka  and Sekulić, Milka I.",
year = "2010",
abstract = "Glucocorticoids have an inhibitory influence on proliferation activity of the pituitary cells while stimulating apoptosis. Therefore, it was hypothesized that the synthetic glucocorticoid, dexamethasone (DX), has an inhibitory influence on the number of thyroid-stimulating hormone (TSH) cells during fetal development. The effects of maternal administration of DX on stereological parameters of TSH cells, and TSH serum concentration were investigated in 21-day-old rat fetuses. On day 16 of pregnancy, the experimental dams received 1.0 mg DX/kg b.w. subcutaneously, followed by 0.5 mg DX/kg b.w./day on days 17 and 18 of gestation. The control gravid females received the same volume of saline vehicle. TSH cells were stained immunocytochemically by the peroxidase-antiperoxidase (PAP) method. The fetal pituitary volumes were estimated using Cavalieri's principle. A physical disector counting technique in combination with the fractionator sampling method was used for estimation of pituitary TSH cell number. Cell and nuclear volumes were measured with a planar rotator. Maternal DX application was found to cause a significant decrease of pituitary volume and number of TSH cells per pituitary in 21-day-old fetuses in comparison with the control fetuses. TSH cell number expressed per body weight unit declined significantly after maternal DX administration. These results indicate an inhibitory DX influence on proliferative activity of precursors and likely differentiated TSH cells and increased apoptotic prevalence. The histological appearance, volume of TSH cells and TSH serum concentration suggest intensive synthetic activity in TSH cells of DX exposed fetuses. Microsc. Res. Tech. 73:1077-1085, 2010. (C) 2010 Wiley-Liss, Inc.",
publisher = "John Wiley and Sons",
journal = "Microscopy Research and Technique",
title = "Unbiased Stereological Estimation of the Rat Fetal Pituitary Volume and of the Total Number and Volume of TSH Cells After Maternal Dexamethasone Application",
number = "12",
volume = "73",
doi = "10.1002/jemt.20842",
pages = "1077-1085",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1332"
}

Manojlović-Stojanoski, M., Nestorović, N., Ristić, N., Trifunović, S., Filipović, B., Šošić-Jurjević, B.,& Sekulić, M. I.. (2010). Unbiased Stereological Estimation of the Rat Fetal Pituitary Volume and of the Total Number and Volume of TSH Cells After Maternal Dexamethasone Application. in Microscopy Research and Technique
John Wiley and Sons., 73(12), 1077-1085.
https://doi.org/10.1002/jemt.20842
https://hdl.handle.net/21.15107/rcub_ibiss_1332

Manojlović-Stojanoski M, Nestorović N, Ristić N, Trifunović S, Filipović B, Šošić-Jurjević B, Sekulić MI. Unbiased Stereological Estimation of the Rat Fetal Pituitary Volume and of the Total Number and Volume of TSH Cells After Maternal Dexamethasone Application. in Microscopy Research and Technique. 2010;73(12):1077-1085.
doi:10.1002/jemt.20842
https://hdl.handle.net/21.15107/rcub_ibiss_1332 .

Manojlović-Stojanoski, Milica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Filipović, Branko, Šošić-Jurjević, Branka , Sekulić, Milka I., "Unbiased Stereological Estimation of the Rat Fetal Pituitary Volume and of the Total Number and Volume of TSH Cells After Maternal Dexamethasone Application" in Microscopy Research and Technique, 73, no. 12 (2010):1077-1085,
https://doi.org/10.1002/jemt.20842 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1332 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Trifunović, Svetlana
AU  - Manojlović-Stojanoski, Milica
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1465
AB  - The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. Besides estrogenic/antiestrogenic action, this isoflavone exerts a prominent inhibitory effect on tyrosine kinase and the steroidogenic enzyme families, thus affecting hormonal homeostasis. The aim of this study was to examine the effects of genistein on: histomorphometric features of the adrenal cortex, blood concentrations of aldosterone, corticosterone and dehydroepiandrosterone (DHEA) and adrenal tissue corticosterone content in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. The adrenal cortex was analysed histologically and morphometrically. Circulating concentrations of aldosterone, corticosterone and DHEA, as well as adrenal tissue corticosterone levels, were determined by immunoassay. When compared to the SO group, orchidectomy decreased the ZG and ZR cell volume by 43% and 29%, respectively (P < 0.05). Serum concentrations of aldosterone and DHEA were markedly lower [13% and 41%, respectively (P < 0.05)], while serum and adrenal tissue levels of corticosterone did not change after orchidectomy. Orchidectomy followed by genistein treatment increased the ZG, ZF and ZR cell volume by 54%, 34% and 77%, respectively (P < 0.05), compared to the untreated orchidectomized group. Histological analysis revealed noticeable vacuolization of the ZG and ZF cells in the Orx+G group. Serum aldosterone and corticosterone concentrations together with adrenal tissue corticosterone were 47%, 31% and 44% lower, respectively (P < 0.05), whereas serum DHEA concentration was 342% higher (P < 0.05) in this group in comparison with the Orx group. This study shows that in orchidectomized middle-aged rats, genistein can cause the shunting of metabolic pathways in the adrenals, supporting DHEA secretion and inhibiting corticosterone and aldosterone secretion. Exp Biol Med 234:148-156, 2009
PB  - Society for Experimental Biology and Medicine
T2  - Experimental Biology and Medicine
T1  - Genistein-Induced Histomorphometric and Hormone Secreting Changes in the Adrenal Cortex in Middle-Aged Rats
IS  - 2
VL  - 234
DO  - 10.3181/0807-RM-231
SP  - 148
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1465
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka  and Filipović, Branko and Trifunović, Svetlana and Manojlović-Stojanoski, Milica and Sekulić, Milka I. and Milošević, Verica",
year = "2009",
abstract = "The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. Besides estrogenic/antiestrogenic action, this isoflavone exerts a prominent inhibitory effect on tyrosine kinase and the steroidogenic enzyme families, thus affecting hormonal homeostasis. The aim of this study was to examine the effects of genistein on: histomorphometric features of the adrenal cortex, blood concentrations of aldosterone, corticosterone and dehydroepiandrosterone (DHEA) and adrenal tissue corticosterone content in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. The adrenal cortex was analysed histologically and morphometrically. Circulating concentrations of aldosterone, corticosterone and DHEA, as well as adrenal tissue corticosterone levels, were determined by immunoassay. When compared to the SO group, orchidectomy decreased the ZG and ZR cell volume by 43% and 29%, respectively (P < 0.05). Serum concentrations of aldosterone and DHEA were markedly lower [13% and 41%, respectively (P < 0.05)], while serum and adrenal tissue levels of corticosterone did not change after orchidectomy. Orchidectomy followed by genistein treatment increased the ZG, ZF and ZR cell volume by 54%, 34% and 77%, respectively (P < 0.05), compared to the untreated orchidectomized group. Histological analysis revealed noticeable vacuolization of the ZG and ZF cells in the Orx+G group. Serum aldosterone and corticosterone concentrations together with adrenal tissue corticosterone were 47%, 31% and 44% lower, respectively (P < 0.05), whereas serum DHEA concentration was 342% higher (P < 0.05) in this group in comparison with the Orx group. This study shows that in orchidectomized middle-aged rats, genistein can cause the shunting of metabolic pathways in the adrenals, supporting DHEA secretion and inhibiting corticosterone and aldosterone secretion. Exp Biol Med 234:148-156, 2009",
publisher = "Society for Experimental Biology and Medicine",
journal = "Experimental Biology and Medicine",
title = "Genistein-Induced Histomorphometric and Hormone Secreting Changes in the Adrenal Cortex in Middle-Aged Rats",
number = "2",
volume = "234",
doi = "10.3181/0807-RM-231",
pages = "148-156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1465"
}

Ajdžanović, V., Šošić-Jurjević, B., Filipović, B., Trifunović, S., Manojlović-Stojanoski, M., Sekulić, M. I.,& Milošević, V.. (2009). Genistein-Induced Histomorphometric and Hormone Secreting Changes in the Adrenal Cortex in Middle-Aged Rats. in Experimental Biology and Medicine
Society for Experimental Biology and Medicine., 234(2), 148-156.
https://doi.org/10.3181/0807-RM-231
https://hdl.handle.net/21.15107/rcub_ibiss_1465

Ajdžanović V, Šošić-Jurjević B, Filipović B, Trifunović S, Manojlović-Stojanoski M, Sekulić MI, Milošević V. Genistein-Induced Histomorphometric and Hormone Secreting Changes in the Adrenal Cortex in Middle-Aged Rats. in Experimental Biology and Medicine. 2009;234(2):148-156.
doi:10.3181/0807-RM-231
https://hdl.handle.net/21.15107/rcub_ibiss_1465 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka , Filipović, Branko, Trifunović, Svetlana, Manojlović-Stojanoski, Milica, Sekulić, Milka I., Milošević, Verica, "Genistein-Induced Histomorphometric and Hormone Secreting Changes in the Adrenal Cortex in Middle-Aged Rats" in Experimental Biology and Medicine, 234, no. 2 (2009):148-156,
https://doi.org/10.3181/0807-RM-231 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1465 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Trifunović, Svetlana
AU  - Brkić, Dejan D
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1467
AB  - The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. However, despite the beneficial effects on health, concern has been raised that this isoflavone also acts as an endocrine-disrupting chemical. The aim of this study was to examine the effects of genistein on immunohistomorphometric features of pituitary adrenocorticotropic cells (ACTH) and blood concentrations of ACTH and corticosterone in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. ACTH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Circulating concentrations of ACTH and corticosterone were measured by immunoassay. Orchidectomy reduced (p<0.05) the cell volume and the relative volume of ACTH cells in comparison to SO rats. Genistein treatment further decreased (p<0.05) these morphometric parameters and reduced (p<0.05) circulating ACTH and corticosterone concentrations by more than 20% in comparison to both Orx and SO rats. In conclusion, genistein modulated the immunohistomorphometric features of ACTH cells and decreased blood ACTH and corticosterone levels, which supports evidence that this isoflavone affects the hypothalamic-pituitary-adrenal axis and suppresses glucocorticoid hormone secretion.
PB  - Sociedad de Biología de Chile
T2  - Biological Research
T1  - Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
IS  - 1
VL  - 42
SP  - 13
EP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1467
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka  and Filipović, Branko and Trifunović, Svetlana and Brkić, Dejan D and Sekulić, Milka I. and Milošević, Verica",
year = "2009",
abstract = "The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. However, despite the beneficial effects on health, concern has been raised that this isoflavone also acts as an endocrine-disrupting chemical. The aim of this study was to examine the effects of genistein on immunohistomorphometric features of pituitary adrenocorticotropic cells (ACTH) and blood concentrations of ACTH and corticosterone in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. ACTH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Circulating concentrations of ACTH and corticosterone were measured by immunoassay. Orchidectomy reduced (p<0.05) the cell volume and the relative volume of ACTH cells in comparison to SO rats. Genistein treatment further decreased (p<0.05) these morphometric parameters and reduced (p<0.05) circulating ACTH and corticosterone concentrations by more than 20% in comparison to both Orx and SO rats. In conclusion, genistein modulated the immunohistomorphometric features of ACTH cells and decreased blood ACTH and corticosterone levels, which supports evidence that this isoflavone affects the hypothalamic-pituitary-adrenal axis and suppresses glucocorticoid hormone secretion.",
publisher = "Sociedad de Biología de Chile",
journal = "Biological Research",
title = "Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats",
number = "1",
volume = "42",
pages = "13-23",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1467"
}

Ajdžanović, V., Šošić-Jurjević, B., Filipović, B., Trifunović, S., Brkić, D. D., Sekulić, M. I.,& Milošević, V.. (2009). Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats. in Biological Research
Sociedad de Biología de Chile., 42(1), 13-23.
https://hdl.handle.net/21.15107/rcub_ibiss_1467

Ajdžanović V, Šošić-Jurjević B, Filipović B, Trifunović S, Brkić DD, Sekulić MI, Milošević V. Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats. in Biological Research. 2009;42(1):13-23.
https://hdl.handle.net/21.15107/rcub_ibiss_1467 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka , Filipović, Branko, Trifunović, Svetlana, Brkić, Dejan D, Sekulić, Milka I., Milošević, Verica, "Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats" in Biological Research, 42, no. 1 (2009):13-23,
https://hdl.handle.net/21.15107/rcub_ibiss_1467 .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Stevanović, Darko M
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
AU  - Starčević, Vesna P.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1486
AB  - Body weight depends on the balance between energy intake and consumption. An interaction between ghrelin and thyroid function has been reported only in pathophysiological states. We examined whether intracerebroventricular (ICV) administration of ghrelin affects the structure and function of the pituitary-thyroid axis in young adult male rats. Ghrelin (0.3 nmol/5 mu l PBS) or an equal volume of PBS were injected every 24 h into the lateral cerebral ventricle for 5 days. Two hours after the last treatment the animals were killed, their pituitaries and thyroids excised and prepared for further histological, immunohistochemical and morphometric investigation. Serum TSH levels were measured by RIA, while the total T(4) and T(3) levels were examined by ECLIA. Ghrelin treatment increased pituitary weight (p < 0.05) when compared to the controls, with no effect on the thyroid weight. Smaller, degranulated TSH-immunopositive cells were noticed within the pituitaries of ghrelin-treated animals; their cellular and nuclear volume as well as the relative volume density of thyrotrophs decreased (p < 0.05) in comparison to the control values. The level of serum TSH was reduced (p < 0.05). In the thyroid parenchyma of ghrelin-treated rats, an increased number of hypofunctioning follicles was noticed, characterized by flattened, weakly Tg-immunoreactive epithelium and colloid distension. The relative volume densities of the follicles and colloid increased (p < 0.05), while the thyroid index of activation rate and the serum level of total T(4) decreased (p < 0.05). In conclusion, centrally applied ghrelin modulated the immunohistomorphometric features of pituitary TSH cells and decreased the level of serum TSH, consequently changing thyroid morphology and function, by reducing the T(4) hormone level in the serum. Copyright (C) 2008 S. Karger AG, Basel
PB  - Karger Publishers
T2  - Neuroendocrinology
T1  - Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats
IS  - 3
VL  - 89
DO  - 10.1159/000188603
SP  - 327
EP  - 336
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1486
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Stevanović, Darko M and Milošević, Verica and Sekulić, Milka I. and Starčević, Vesna P.",
year = "2009",
abstract = "Body weight depends on the balance between energy intake and consumption. An interaction between ghrelin and thyroid function has been reported only in pathophysiological states. We examined whether intracerebroventricular (ICV) administration of ghrelin affects the structure and function of the pituitary-thyroid axis in young adult male rats. Ghrelin (0.3 nmol/5 mu l PBS) or an equal volume of PBS were injected every 24 h into the lateral cerebral ventricle for 5 days. Two hours after the last treatment the animals were killed, their pituitaries and thyroids excised and prepared for further histological, immunohistochemical and morphometric investigation. Serum TSH levels were measured by RIA, while the total T(4) and T(3) levels were examined by ECLIA. Ghrelin treatment increased pituitary weight (p < 0.05) when compared to the controls, with no effect on the thyroid weight. Smaller, degranulated TSH-immunopositive cells were noticed within the pituitaries of ghrelin-treated animals; their cellular and nuclear volume as well as the relative volume density of thyrotrophs decreased (p < 0.05) in comparison to the control values. The level of serum TSH was reduced (p < 0.05). In the thyroid parenchyma of ghrelin-treated rats, an increased number of hypofunctioning follicles was noticed, characterized by flattened, weakly Tg-immunoreactive epithelium and colloid distension. The relative volume densities of the follicles and colloid increased (p < 0.05), while the thyroid index of activation rate and the serum level of total T(4) decreased (p < 0.05). In conclusion, centrally applied ghrelin modulated the immunohistomorphometric features of pituitary TSH cells and decreased the level of serum TSH, consequently changing thyroid morphology and function, by reducing the T(4) hormone level in the serum. Copyright (C) 2008 S. Karger AG, Basel",
publisher = "Karger Publishers",
journal = "Neuroendocrinology",
title = "Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats",
number = "3",
volume = "89",
doi = "10.1159/000188603",
pages = "327-336",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1486"
}

Šošić-Jurjević, B., Stevanović, D. M., Milošević, V., Sekulić, M. I.,& Starčević, V. P.. (2009). Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats. in Neuroendocrinology
Karger Publishers., 89(3), 327-336.
https://doi.org/10.1159/000188603
https://hdl.handle.net/21.15107/rcub_ibiss_1486

Šošić-Jurjević B, Stevanović DM, Milošević V, Sekulić MI, Starčević VP. Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats. in Neuroendocrinology. 2009;89(3):327-336.
doi:10.1159/000188603
https://hdl.handle.net/21.15107/rcub_ibiss_1486 .

Šošić-Jurjević, Branka , Stevanović, Darko M, Milošević, Verica, Sekulić, Milka I., Starčević, Vesna P., "Central Ghrelin Affects Pituitary-Thyroid Axis: Histomorphological and Hormonal Study in Rats" in Neuroendocrinology, 89, no. 3 (2009):327-336,
https://doi.org/10.1159/000188603 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1486 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Brkić, Marjana P.
AU  - Nestorović, Nataša
AU  - Sekulić, Milka I.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1462
AB  - The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases. The aim of this study was to examine the morphofunctional characteristics of adrenocorticotrophic (ACTH) cells and blood concentrations of ACTH in sham-operated, orchidectomized and genistein-treated orchidectomized, 16-month-old Wistar male rats. Genistein (10 mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. Orchidectomy and genistein treatment decreased the volume density of ACTH cells and reduced (p < 0.05) circulating ACTH concentrations in comparison with control groups. In conclusion, genistein modulated the morphofunctional features of ACTH cells and decreased blood ACTH levels.
PB  - Bratislava: Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences
T2  - General Physiology and Biophysics
T1  - Morphofunctional characteristics of ACTH cells in middle-aged male rats after treatment with genistein
IS  - 1
VL  - 28
DO  - 10.4149/gpb_2009_01_94
SP  - 94
EP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1462
ER  - 

@article{
author = "Milošević, Verica and Ajdžanović, Vladimir and Šošić-Jurjević, Branka  and Filipović, Branko and Brkić, Marjana P. and Nestorović, Nataša and Sekulić, Milka I.",
year = "2009",
abstract = "The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases. The aim of this study was to examine the morphofunctional characteristics of adrenocorticotrophic (ACTH) cells and blood concentrations of ACTH in sham-operated, orchidectomized and genistein-treated orchidectomized, 16-month-old Wistar male rats. Genistein (10 mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. Orchidectomy and genistein treatment decreased the volume density of ACTH cells and reduced (p < 0.05) circulating ACTH concentrations in comparison with control groups. In conclusion, genistein modulated the morphofunctional features of ACTH cells and decreased blood ACTH levels.",
publisher = "Bratislava: Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences",
journal = "General Physiology and Biophysics",
title = "Morphofunctional characteristics of ACTH cells in middle-aged male rats after treatment with genistein",
number = "1",
volume = "28",
doi = "10.4149/gpb_2009_01_94",
pages = "94-97",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1462"
}

Milošević, V., Ajdžanović, V., Šošić-Jurjević, B., Filipović, B., Brkić, M. P., Nestorović, N.,& Sekulić, M. I.. (2009). Morphofunctional characteristics of ACTH cells in middle-aged male rats after treatment with genistein. in General Physiology and Biophysics
Bratislava: Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences., 28(1), 94-97.
https://doi.org/10.4149/gpb_2009_01_94
https://hdl.handle.net/21.15107/rcub_ibiss_1462

Milošević V, Ajdžanović V, Šošić-Jurjević B, Filipović B, Brkić MP, Nestorović N, Sekulić MI. Morphofunctional characteristics of ACTH cells in middle-aged male rats after treatment with genistein. in General Physiology and Biophysics. 2009;28(1):94-97.
doi:10.4149/gpb_2009_01_94
https://hdl.handle.net/21.15107/rcub_ibiss_1462 .

Milošević, Verica, Ajdžanović, Vladimir, Šošić-Jurjević, Branka , Filipović, Branko, Brkić, Marjana P., Nestorović, Nataša, Sekulić, Milka I., "Morphofunctional characteristics of ACTH cells in middle-aged male rats after treatment with genistein" in General Physiology and Biophysics, 28, no. 1 (2009):94-97,
https://doi.org/10.4149/gpb_2009_01_94 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1462 .

TY  - JOUR
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Nestorović, Nataša
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1516
AB  - Somatostatin analogues are currently used to treat various disorders such as hypersecretion and different neuroendocrine tumors. In this study we examined the effects on the adrenal cortex of somatostatin (SRIH-14) and octreotide administered subcutaneously twice daily for 5 days to adult male rats. Control rats received saline under the same regime. After sacrifice, the adrenal glands were removed and examined morphometrically using the M(42) multipurpose test system. Blood samples were prepared for biochemical tests. Both SRIH-14 and octreotide induced morphofunctional changes in adrenal zona glomerulosa. We found significant decreases (p < 0.05) in the absolute cell and nuclear volumes of zona glomerulosa in both experimental groups in comparison to the control. The serum aldosterone level was 11% lower (p < 0.05) in the SRIH-14 and 13% (p < 0.05) lower in the octreotide-treated group in comparison with the control group. Morphometric parameters of zona fasciculata and zona reticulata and corticosterone levels were not altered significantly (p > 0.05) in either treated group. It may therefore be concluded that both SRIH-14 and octreotide affected zona glomerulosa in the same manner by decreasing morphofunctional characteristics.
PB  - Slovak Academy of Sciences, Institute of Molecular Physiology and Genetics
T2  - General Physiology and Biophysics
T1  - Multiple treatments with SRIH-14 or octreotide affect adrenal zona glomerulosa in adult male rats
IS  - 3
VL  - 27
SP  - 159
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1516
ER  - 

@article{
author = "Trifunović, Svetlana and Ajdžanović, Vladimir and Šošić-Jurjević, Branka  and Filipović, Branko and Nestorović, Nataša and Sekulić, Milka I. and Milošević, Verica",
year = "2008",
abstract = "Somatostatin analogues are currently used to treat various disorders such as hypersecretion and different neuroendocrine tumors. In this study we examined the effects on the adrenal cortex of somatostatin (SRIH-14) and octreotide administered subcutaneously twice daily for 5 days to adult male rats. Control rats received saline under the same regime. After sacrifice, the adrenal glands were removed and examined morphometrically using the M(42) multipurpose test system. Blood samples were prepared for biochemical tests. Both SRIH-14 and octreotide induced morphofunctional changes in adrenal zona glomerulosa. We found significant decreases (p < 0.05) in the absolute cell and nuclear volumes of zona glomerulosa in both experimental groups in comparison to the control. The serum aldosterone level was 11% lower (p < 0.05) in the SRIH-14 and 13% (p < 0.05) lower in the octreotide-treated group in comparison with the control group. Morphometric parameters of zona fasciculata and zona reticulata and corticosterone levels were not altered significantly (p > 0.05) in either treated group. It may therefore be concluded that both SRIH-14 and octreotide affected zona glomerulosa in the same manner by decreasing morphofunctional characteristics.",
publisher = "Slovak Academy of Sciences, Institute of Molecular Physiology and Genetics",
journal = "General Physiology and Biophysics",
title = "Multiple treatments with SRIH-14 or octreotide affect adrenal zona glomerulosa in adult male rats",
number = "3",
volume = "27",
pages = "159-163",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1516"
}

Trifunović, S., Ajdžanović, V., Šošić-Jurjević, B., Filipović, B., Nestorović, N., Sekulić, M. I.,& Milošević, V.. (2008). Multiple treatments with SRIH-14 or octreotide affect adrenal zona glomerulosa in adult male rats. in General Physiology and Biophysics
Slovak Academy of Sciences, Institute of Molecular Physiology and Genetics., 27(3), 159-163.
https://hdl.handle.net/21.15107/rcub_ibiss_1516

Trifunović S, Ajdžanović V, Šošić-Jurjević B, Filipović B, Nestorović N, Sekulić MI, Milošević V. Multiple treatments with SRIH-14 or octreotide affect adrenal zona glomerulosa in adult male rats. in General Physiology and Biophysics. 2008;27(3):159-163.
https://hdl.handle.net/21.15107/rcub_ibiss_1516 .

Trifunović, Svetlana, Ajdžanović, Vladimir, Šošić-Jurjević, Branka , Filipović, Branko, Nestorović, Nataša, Sekulić, Milka I., Milošević, Verica, "Multiple treatments with SRIH-14 or octreotide affect adrenal zona glomerulosa in adult male rats" in General Physiology and Biophysics, 27, no. 3 (2008):159-163,
https://hdl.handle.net/21.15107/rcub_ibiss_1516 .

TY  - JOUR
AU  - Trifunović, Svetlana
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka 
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1555
AB  - The effects of chronic treatments with SRIH-14 and octreotide on pituitary corticotropes (ACTH cells) and on the adrenal cortex of male Wistar rats were examined. Adult males received two daily s.c. injections of 20 mu g/100 g of body weight of either SRIH-14 or octreotide for 28 consecutive days. ACTH cells were studied using a peroxidase-antiperoxidase immunocytochemical procedure. Morphometry was used to evaluate the changes in cell and nuclear volumes (mu m(3)) and volume densities (%) of ACTH-immunoreactive cells. The adrenal cortex was analyzed by histological and morphometric methods. A significant (p<0.05) decrease in body weight and in the absolute weights of the pituitary and adrenal glands was observed in both treated groups. Morphometric parameters of ACTH cells in both treated groups were not significantly (p>0.05) different than in control rats. The absolute volumes of the adrenal gland and adrenal cortex were significantly (p<0.05) decreased in both treated groups. The absolute and relative volumes of the zona glomerulosa (ZG), as well as the cellular and nuclear volumes of the ZG were significantly (p<0.05) decreased in the both treated groups. In rats treated with SRIH-14 and octreotide, the absolute and relative volumes of the zona fasciculata (ZF) and zona reticularis (ZR), as well as their stereological parameters, did not change significantly (p>0.05). The aldosterone levels in the SRIH-14 and ocreotide-treated groups were significantly (p<0.05) decreased - by 13% and 19%, respectively. The concentration of ACTH and corticosterone did not change significantly. Together, these findings show that SRIH-14 and octreotide administration affected the morphological characteristics of the adrenal ZG in a similar manner, and brought about a decrease in plasma aldosterone concentration. These treatments did not affect pituitary ACTH cells or adrenal ZF and ZR functioning.
PB  - PAGEPress
T2  - European Journal of Histochemistry
T1  - The effects of chronic SRIH-14 and octreotide administration on the pituitary-adrenal axis in adult male rats
IS  - 1
VL  - 52
DO  - 10.4081/1187
SP  - 61
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1555
ER  - 

@article{
author = "Trifunović, Svetlana and Filipović, Branko and Ajdžanović, Vladimir and Šošić-Jurjević, Branka  and Manojlović-Stojanoski, Milica and Nestorović, Nataša and Ristić, Nataša and Sekulić, Milka I. and Milošević, Verica",
year = "2008",
abstract = "The effects of chronic treatments with SRIH-14 and octreotide on pituitary corticotropes (ACTH cells) and on the adrenal cortex of male Wistar rats were examined. Adult males received two daily s.c. injections of 20 mu g/100 g of body weight of either SRIH-14 or octreotide for 28 consecutive days. ACTH cells were studied using a peroxidase-antiperoxidase immunocytochemical procedure. Morphometry was used to evaluate the changes in cell and nuclear volumes (mu m(3)) and volume densities (%) of ACTH-immunoreactive cells. The adrenal cortex was analyzed by histological and morphometric methods. A significant (p<0.05) decrease in body weight and in the absolute weights of the pituitary and adrenal glands was observed in both treated groups. Morphometric parameters of ACTH cells in both treated groups were not significantly (p>0.05) different than in control rats. The absolute volumes of the adrenal gland and adrenal cortex were significantly (p<0.05) decreased in both treated groups. The absolute and relative volumes of the zona glomerulosa (ZG), as well as the cellular and nuclear volumes of the ZG were significantly (p<0.05) decreased in the both treated groups. In rats treated with SRIH-14 and octreotide, the absolute and relative volumes of the zona fasciculata (ZF) and zona reticularis (ZR), as well as their stereological parameters, did not change significantly (p>0.05). The aldosterone levels in the SRIH-14 and ocreotide-treated groups were significantly (p<0.05) decreased - by 13% and 19%, respectively. The concentration of ACTH and corticosterone did not change significantly. Together, these findings show that SRIH-14 and octreotide administration affected the morphological characteristics of the adrenal ZG in a similar manner, and brought about a decrease in plasma aldosterone concentration. These treatments did not affect pituitary ACTH cells or adrenal ZF and ZR functioning.",
publisher = "PAGEPress",
journal = "European Journal of Histochemistry",
title = "The effects of chronic SRIH-14 and octreotide administration on the pituitary-adrenal axis in adult male rats",
number = "1",
volume = "52",
doi = "10.4081/1187",
pages = "61-68",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1555"
}

Trifunović, S., Filipović, B., Ajdžanović, V., Šošić-Jurjević, B., Manojlović-Stojanoski, M., Nestorović, N., Ristić, N., Sekulić, M. I.,& Milošević, V.. (2008). The effects of chronic SRIH-14 and octreotide administration on the pituitary-adrenal axis in adult male rats. in European Journal of Histochemistry
PAGEPress., 52(1), 61-68.
https://doi.org/10.4081/1187
https://hdl.handle.net/21.15107/rcub_ibiss_1555

Trifunović S, Filipović B, Ajdžanović V, Šošić-Jurjević B, Manojlović-Stojanoski M, Nestorović N, Ristić N, Sekulić MI, Milošević V. The effects of chronic SRIH-14 and octreotide administration on the pituitary-adrenal axis in adult male rats. in European Journal of Histochemistry. 2008;52(1):61-68.
doi:10.4081/1187
https://hdl.handle.net/21.15107/rcub_ibiss_1555 .

Trifunović, Svetlana, Filipović, Branko, Ajdžanović, Vladimir, Šošić-Jurjević, Branka , Manojlović-Stojanoski, Milica, Nestorović, Nataša, Ristić, Nataša, Sekulić, Milka I., Milošević, Verica, "The effects of chronic SRIH-14 and octreotide administration on the pituitary-adrenal axis in adult male rats" in European Journal of Histochemistry, 52, no. 1 (2008):61-68,
https://doi.org/10.4081/1187 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1555 .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1503
AB  - Elevated glucocorticoid levels in the gravid female circulation affect a number of endocrine functions in the fetuses and neonates. The aim of this study was to examine the effects of maternal dexamethasone (Dx) administration during late pregnancy on the ovaries of neonatal offspring. On the 16th day of pregnancy, experimental dams received subcutaneously 1.0 mg Dx/kg b.w., followed by 0.5 mg Dx/kg b.w./day on the 17th and 18th days of gestation. The control gravid females received the same volume of saline vehicle. Left ovaries from 5-day-old female pups were stereologically analyzed. The ovary volumes were estimated using Cavalieri's principle. The number of healthy and atretic primordial and primary follicles was estimated using a fractionator-physical disector method. The number of secondary follicles was determined by exact counts of every fourth section encompassing whole cross-sections of the ovary. The ovary volume was significantly decreased (by 44.4%; P < 0.05) in the group of female pups from Dx-treated mothers comparing to the controls. The numbers of healthy primordial and atretic follicles were 38.8% (P < 0.05) and 50.9% (P < 0.05), respectively, reduced in the ovaries of pups from the Dx-treated mothers, when compared with the control values. There were 53.4% (P < 0.05) fewer healthy primary and 41.8% (P < 0.05) fewer healthy secondary follicles as well. The numbers of atretic primary and secondary follicles were reduced by 60.0% (P < 0.05) and 61.7% (P < 0.05), respectively. It can be concluded that fetal exposure to glucocorticoids decreased the pool of non-growing follicles in the neonatal ovary, whereas the processes of folliculogenesis and atresia remained unaffected.
PB  - Blackwell Publishing Ltd
T2  - Journal of Microscopy
T1  - Maternal dexamethasone treatment reduces ovarian follicle number in neonatal rat offspring
IS  - 3
VL  - 232
DO  - 10.1111/j.1365-2818.2008.02117.x
SP  - 549
EP  - 557
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1503
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Filipović, Branko and Šošić-Jurjević, Branka  and Milošević, Verica and Sekulić, Milka I.",
year = "2008",
abstract = "Elevated glucocorticoid levels in the gravid female circulation affect a number of endocrine functions in the fetuses and neonates. The aim of this study was to examine the effects of maternal dexamethasone (Dx) administration during late pregnancy on the ovaries of neonatal offspring. On the 16th day of pregnancy, experimental dams received subcutaneously 1.0 mg Dx/kg b.w., followed by 0.5 mg Dx/kg b.w./day on the 17th and 18th days of gestation. The control gravid females received the same volume of saline vehicle. Left ovaries from 5-day-old female pups were stereologically analyzed. The ovary volumes were estimated using Cavalieri's principle. The number of healthy and atretic primordial and primary follicles was estimated using a fractionator-physical disector method. The number of secondary follicles was determined by exact counts of every fourth section encompassing whole cross-sections of the ovary. The ovary volume was significantly decreased (by 44.4%; P < 0.05) in the group of female pups from Dx-treated mothers comparing to the controls. The numbers of healthy primordial and atretic follicles were 38.8% (P < 0.05) and 50.9% (P < 0.05), respectively, reduced in the ovaries of pups from the Dx-treated mothers, when compared with the control values. There were 53.4% (P < 0.05) fewer healthy primary and 41.8% (P < 0.05) fewer healthy secondary follicles as well. The numbers of atretic primary and secondary follicles were reduced by 60.0% (P < 0.05) and 61.7% (P < 0.05), respectively. It can be concluded that fetal exposure to glucocorticoids decreased the pool of non-growing follicles in the neonatal ovary, whereas the processes of folliculogenesis and atresia remained unaffected.",
publisher = "Blackwell Publishing Ltd",
journal = "Journal of Microscopy",
title = "Maternal dexamethasone treatment reduces ovarian follicle number in neonatal rat offspring",
number = "3",
volume = "232",
doi = "10.1111/j.1365-2818.2008.02117.x",
pages = "549-557",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1503"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Filipović, B., Šošić-Jurjević, B., Milošević, V.,& Sekulić, M. I.. (2008). Maternal dexamethasone treatment reduces ovarian follicle number in neonatal rat offspring. in Journal of Microscopy
Blackwell Publishing Ltd., 232(3), 549-557.
https://doi.org/10.1111/j.1365-2818.2008.02117.x
https://hdl.handle.net/21.15107/rcub_ibiss_1503

Ristić N, Nestorović N, Manojlović-Stojanoski M, Filipović B, Šošić-Jurjević B, Milošević V, Sekulić MI. Maternal dexamethasone treatment reduces ovarian follicle number in neonatal rat offspring. in Journal of Microscopy. 2008;232(3):549-557.
doi:10.1111/j.1365-2818.2008.02117.x
https://hdl.handle.net/21.15107/rcub_ibiss_1503 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Filipović, Branko, Šošić-Jurjević, Branka , Milošević, Verica, Sekulić, Milka I., "Maternal dexamethasone treatment reduces ovarian follicle number in neonatal rat offspring" in Journal of Microscopy, 232, no. 3 (2008):549-557,
https://doi.org/10.1111/j.1365-2818.2008.02117.x .,
https://hdl.handle.net/21.15107/rcub_ibiss_1503 .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1494
AB  - Growth hormone (GH) and glucocorticoids have a powerful influence on controlling fetal growth, differentiation and maturation of numerous tissues. In the present study, the effect of maternal dexamethasone (Dx) treatment on GH cells and body weight in 19- and 21-day-old rat fetuses was investigated using immunocytochemical and morphometric methods. Pregnant female rats received daily injections of 1.0-0.5-0.5 mg Dx/kg b.w. on days 16-18 of pregnancy (experimental group), while the control group received an equal volume of saline. Dx treatment of pregnant rats enhanced immunostaining intensity and significantly increased (p < 0.05) GH nuclear and cell volume, as well as volume density and number of GH cells per square millimeter in 19-day-old fetuses compared to the controls. In 21-day-old fetuses after maternal Dx administration, immunoreactivity, volume density and number of GH cells remained significantly increased (p < 0.05). Dx treatment of pregnant rats resulted in marked body weight reduction of 21-day-old but not 19 days old fetuses in comparison with the corresponding controls. The presented results demonstrate that maternal Dx application has pronounced effect on morphometric parameters of GH cells of 19- and 21-day-old fetuses. Also, in near-term rat fetuses body weight was largely independent of pituitary GH cell activity.
PB  - Polish Histochemical and Cytochemical Society
T2  - Folia Histochemica et Cytobiologica
T1  - Influence of maternal dexamethasone treatment on morphometric characteristics of pituitary GH cells and body weight in near-term rat fetuses
IS  - 1
VL  - 45
SP  - 51
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1494
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Nestorović, Nataša and Ristić, Nataša and Filipović, Branko and Šošić-Jurjević, Branka  and Sekulić, Milka I. and Milošević, Verica",
year = "2007",
abstract = "Growth hormone (GH) and glucocorticoids have a powerful influence on controlling fetal growth, differentiation and maturation of numerous tissues. In the present study, the effect of maternal dexamethasone (Dx) treatment on GH cells and body weight in 19- and 21-day-old rat fetuses was investigated using immunocytochemical and morphometric methods. Pregnant female rats received daily injections of 1.0-0.5-0.5 mg Dx/kg b.w. on days 16-18 of pregnancy (experimental group), while the control group received an equal volume of saline. Dx treatment of pregnant rats enhanced immunostaining intensity and significantly increased (p < 0.05) GH nuclear and cell volume, as well as volume density and number of GH cells per square millimeter in 19-day-old fetuses compared to the controls. In 21-day-old fetuses after maternal Dx administration, immunoreactivity, volume density and number of GH cells remained significantly increased (p < 0.05). Dx treatment of pregnant rats resulted in marked body weight reduction of 21-day-old but not 19 days old fetuses in comparison with the corresponding controls. The presented results demonstrate that maternal Dx application has pronounced effect on morphometric parameters of GH cells of 19- and 21-day-old fetuses. Also, in near-term rat fetuses body weight was largely independent of pituitary GH cell activity.",
publisher = "Polish Histochemical and Cytochemical Society",
journal = "Folia Histochemica et Cytobiologica",
title = "Influence of maternal dexamethasone treatment on morphometric characteristics of pituitary GH cells and body weight in near-term rat fetuses",
number = "1",
volume = "45",
pages = "51-56",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1494"
}

Manojlović-Stojanoski, M., Nestorović, N., Ristić, N., Filipović, B., Šošić-Jurjević, B., Sekulić, M. I.,& Milošević, V.. (2007). Influence of maternal dexamethasone treatment on morphometric characteristics of pituitary GH cells and body weight in near-term rat fetuses. in Folia Histochemica et Cytobiologica
Polish Histochemical and Cytochemical Society., 45(1), 51-56.
https://hdl.handle.net/21.15107/rcub_ibiss_1494

Manojlović-Stojanoski M, Nestorović N, Ristić N, Filipović B, Šošić-Jurjević B, Sekulić MI, Milošević V. Influence of maternal dexamethasone treatment on morphometric characteristics of pituitary GH cells and body weight in near-term rat fetuses. in Folia Histochemica et Cytobiologica. 2007;45(1):51-56.
https://hdl.handle.net/21.15107/rcub_ibiss_1494 .

Manojlović-Stojanoski, Milica, Nestorović, Nataša, Ristić, Nataša, Filipović, Branko, Šošić-Jurjević, Branka , Sekulić, Milka I., Milošević, Verica, "Influence of maternal dexamethasone treatment on morphometric characteristics of pituitary GH cells and body weight in near-term rat fetuses" in Folia Histochemica et Cytobiologica, 45, no. 1 (2007):51-56,
https://hdl.handle.net/21.15107/rcub_ibiss_1494 .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Trifunović, Svetlana
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1611
AB  - The effects of pregnancy and multiple dexamethasone (Dx) treatment on morphometric parameters of adenohypophyseal gonadotropic cells that produce follicle stimulating hormone (FSH) and luteinizing hormone (LH) were investigated in female Wistar rats. The rats in the experimental group received injections of 1.0, 0.5 and 0.5mg Dx/kg b.w. on days 16-18 of pregnancy, white the control group received equal volumes of saline. There was also an age-matched adult virgin control group. The experimental and control animals were sacrificed 24 and 72h after the last injection. Using the peroxidase-anti-peroxidase immunohistochemical labeling procedure, morphometric analyses showed that cell volume and volume density of FSH and LH cells on day 19 of pregnancy, as well as the number of LH cells, were significantly decreased compared to the virgin control values. On day 21 of gestation, the volume of FSH and LH cells remained smaller than in the virgin controls. Moreover, FSH and LH cell volume was significantly decreased 24h after multiple Dx treatment in comparison with the pregnant controls. Thus, during the last days of pregnancy, the morphometric parameters of gonadotropic cells decreased in comparison with the control virgin rats, but Dx treatment of pregnant rats had an inhibitory influence on FSH and LH cell size only 24h after the last dose. (c) 2007 Elsevier GmbH. All rights reserved.
PB  - Elsevier GmbH
T2  - Acta Histochemica
T1  - Pregnancy and dexamethasone: Effects on morphometric parameters of gonadotropic cells in rats
IS  - 3
VL  - 109
DO  - 10.1016/j.acthis.2007.02.003
SP  - 185
EP  - 192
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1611
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Filipović, Branko and Šošić-Jurjević, Branka  and Trifunović, Svetlana and Milošević, Verica and Sekulić, Milka I.",
year = "2007",
abstract = "The effects of pregnancy and multiple dexamethasone (Dx) treatment on morphometric parameters of adenohypophyseal gonadotropic cells that produce follicle stimulating hormone (FSH) and luteinizing hormone (LH) were investigated in female Wistar rats. The rats in the experimental group received injections of 1.0, 0.5 and 0.5mg Dx/kg b.w. on days 16-18 of pregnancy, white the control group received equal volumes of saline. There was also an age-matched adult virgin control group. The experimental and control animals were sacrificed 24 and 72h after the last injection. Using the peroxidase-anti-peroxidase immunohistochemical labeling procedure, morphometric analyses showed that cell volume and volume density of FSH and LH cells on day 19 of pregnancy, as well as the number of LH cells, were significantly decreased compared to the virgin control values. On day 21 of gestation, the volume of FSH and LH cells remained smaller than in the virgin controls. Moreover, FSH and LH cell volume was significantly decreased 24h after multiple Dx treatment in comparison with the pregnant controls. Thus, during the last days of pregnancy, the morphometric parameters of gonadotropic cells decreased in comparison with the control virgin rats, but Dx treatment of pregnant rats had an inhibitory influence on FSH and LH cell size only 24h after the last dose. (c) 2007 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier GmbH",
journal = "Acta Histochemica",
title = "Pregnancy and dexamethasone: Effects on morphometric parameters of gonadotropic cells in rats",
number = "3",
volume = "109",
doi = "10.1016/j.acthis.2007.02.003",
pages = "185-192",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1611"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Filipović, B., Šošić-Jurjević, B., Trifunović, S., Milošević, V.,& Sekulić, M. I.. (2007). Pregnancy and dexamethasone: Effects on morphometric parameters of gonadotropic cells in rats. in Acta Histochemica
Elsevier GmbH., 109(3), 185-192.
https://doi.org/10.1016/j.acthis.2007.02.003
https://hdl.handle.net/21.15107/rcub_ibiss_1611

Ristić N, Nestorović N, Manojlović-Stojanoski M, Filipović B, Šošić-Jurjević B, Trifunović S, Milošević V, Sekulić MI. Pregnancy and dexamethasone: Effects on morphometric parameters of gonadotropic cells in rats. in Acta Histochemica. 2007;109(3):185-192.
doi:10.1016/j.acthis.2007.02.003
https://hdl.handle.net/21.15107/rcub_ibiss_1611 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Filipović, Branko, Šošić-Jurjević, Branka , Trifunović, Svetlana, Milošević, Verica, Sekulić, Milka I., "Pregnancy and dexamethasone: Effects on morphometric parameters of gonadotropic cells in rats" in Acta Histochemica, 109, no. 3 (2007):185-192,
https://doi.org/10.1016/j.acthis.2007.02.003 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1611 .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Šošić-Jurjević, Branka 
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1664
AB  - Exposure to glucocorticoids leads to numerous changes in various biological systems including the reproductive system. The aim of the present work was to find out whether dexamethasone (Dx) treatment of adult female rats would influence the histological and morphometric characteristics of the pituitary gonadotrophic cells (luteinizing - LH cells and follicle stimulating - FSH cells). One group of female Wistar rats received Dx injections on three consecutive days in doses 1.0, 0.5 and 0.5 mg/kg b.w. respectively, while the control rats were treated with equivalent volumes of saline. Experimental and control animals were sacrificed 24 h and 72 h after the last injection. The peroxidase-antiperoxidase (PAP) immunocytochemical procedure was used to study the LH and FSH cells. The stereological and morphometric analyses showed that multiple Dx treatments of female rats significantly decreased the volume of LH cells and the volume of their nuclei 24 h and 72 h after the last Dx injection in comparison with control values. At 24 h after Dx treatment, the volume density of LH cells was significantly increased, but at 72 h differences between the experimental and control groups were insignificant. The increase in number of LH cells per unit area (mm(2)) was significant at both timepoints (24 h and 72 h). Stereologic and morphometric characteristics of FSH cells was changed after Dx treatment in the same manner as that of LH cells, except for the volume density, where a significant increase was established 24 h and 72 h after the last Dx application. These results clearly demonstrate that 24 h and 72 h after the last of three Dx injections there were changes in the immunocytochemical and morphometric features of gonadotrophic cells.
PB  - Polish Histochemical and Cytochemical Society
T2  - Folia Histochemica et Cytobiologica
T1  - Multiple dexamethasone treatment affects morphometric parameters of gonadotrophic cells in adult female rats
IS  - 2
VL  - 44
SP  - 87
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1664
ER  - 

@article{
author = "Ristić, Nataša and Šošić-Jurjević, Branka  and Manojlović-Stojanoski, Milica and Nestorović, Nataša and Filipović, Branko and Milošević, Verica and Sekulić, Milka I.",
year = "2006",
abstract = "Exposure to glucocorticoids leads to numerous changes in various biological systems including the reproductive system. The aim of the present work was to find out whether dexamethasone (Dx) treatment of adult female rats would influence the histological and morphometric characteristics of the pituitary gonadotrophic cells (luteinizing - LH cells and follicle stimulating - FSH cells). One group of female Wistar rats received Dx injections on three consecutive days in doses 1.0, 0.5 and 0.5 mg/kg b.w. respectively, while the control rats were treated with equivalent volumes of saline. Experimental and control animals were sacrificed 24 h and 72 h after the last injection. The peroxidase-antiperoxidase (PAP) immunocytochemical procedure was used to study the LH and FSH cells. The stereological and morphometric analyses showed that multiple Dx treatments of female rats significantly decreased the volume of LH cells and the volume of their nuclei 24 h and 72 h after the last Dx injection in comparison with control values. At 24 h after Dx treatment, the volume density of LH cells was significantly increased, but at 72 h differences between the experimental and control groups were insignificant. The increase in number of LH cells per unit area (mm(2)) was significant at both timepoints (24 h and 72 h). Stereologic and morphometric characteristics of FSH cells was changed after Dx treatment in the same manner as that of LH cells, except for the volume density, where a significant increase was established 24 h and 72 h after the last Dx application. These results clearly demonstrate that 24 h and 72 h after the last of three Dx injections there were changes in the immunocytochemical and morphometric features of gonadotrophic cells.",
publisher = "Polish Histochemical and Cytochemical Society",
journal = "Folia Histochemica et Cytobiologica",
title = "Multiple dexamethasone treatment affects morphometric parameters of gonadotrophic cells in adult female rats",
number = "2",
volume = "44",
pages = "87-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1664"
}

Ristić, N., Šošić-Jurjević, B., Manojlović-Stojanoski, M., Nestorović, N., Filipović, B., Milošević, V.,& Sekulić, M. I.. (2006). Multiple dexamethasone treatment affects morphometric parameters of gonadotrophic cells in adult female rats. in Folia Histochemica et Cytobiologica
Polish Histochemical and Cytochemical Society., 44(2), 87-92.
https://hdl.handle.net/21.15107/rcub_ibiss_1664

Ristić N, Šošić-Jurjević B, Manojlović-Stojanoski M, Nestorović N, Filipović B, Milošević V, Sekulić MI. Multiple dexamethasone treatment affects morphometric parameters of gonadotrophic cells in adult female rats. in Folia Histochemica et Cytobiologica. 2006;44(2):87-92.
https://hdl.handle.net/21.15107/rcub_ibiss_1664 .

Ristić, Nataša, Šošić-Jurjević, Branka , Manojlović-Stojanoski, Milica, Nestorović, Nataša, Filipović, Branko, Milošević, Verica, Sekulić, Milka I., "Multiple dexamethasone treatment affects morphometric parameters of gonadotrophic cells in adult female rats" in Folia Histochemica et Cytobiologica, 44, no. 2 (2006):87-92,
https://hdl.handle.net/21.15107/rcub_ibiss_1664 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Manojlović-Stojanoski, Milica
AU  - Milošević, Verica
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1658
AB  - Different hormones and growth factors control the homeostasis of the anterior pituitary gland. We examined the morphological features of pituitary thyroid stimulating hormone (TSH)-producing cells in juvenile and peripubertal female rats after treatments with estradiol-dipropionate (EDP), human chorionic gonadotropin (hCG) and a combination of both hormones (EDP+hCG). TSH-producing cells were labelled using a peroxidase-antiperoxidase immunohistochemical procedure for binding of a rabbit anti-rat beta-thyrotropic polyclonal antisera. Morphometric differences in the cytoplasmic and nuclear volume densities in thyrotropes after the treatments were determined using a stereological method. The relative weights of the pituitary glands were significantly higher in the EDP- and EDP+hCG-treated juvenile and peripubertal rats than in untreated age-matched controls. Treatment with EDP promoted a decrease, and treatment with hCG an increase, of the cellular and nuclear volumes in TSH cells in both juvenile and peripubertal females in comparison with the respective controls. Treatment with a combination of EDP+hCG did not induce any significant changes. The cytoplasmic and nuclear volume densities in TSH cells in the EDP+hCG-treated group were significantly higher than in the EDP-treated, and significantly lower than in hCG-treated rats at both growth stages. These findings suggest that estradiol and hCG exerted opposite effects on pituitary TSH-immunoreactive cells. The observed effects on thyrotrope morphology were apparently independent of the stage of development. (C) 2006 Elsevier GmbH. All rights reserved.
PB  - Elsevier GmbH
T2  - Acta Histochemica
T1  - Immunoreactive TSH cells in juvenile and peripubertal rats after estradiol and human chorionic gonadotropin treatment
IS  - 2
VL  - 108
DO  - 10.1016/j.acthis.2006.03.004
SP  - 117
EP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1658
ER  - 

@article{
author = "Sekulić, Milka I. and Šošić-Jurjević, Branka  and Filipović, Branko and Manojlović-Stojanoski, Milica and Milošević, Verica",
year = "2006",
abstract = "Different hormones and growth factors control the homeostasis of the anterior pituitary gland. We examined the morphological features of pituitary thyroid stimulating hormone (TSH)-producing cells in juvenile and peripubertal female rats after treatments with estradiol-dipropionate (EDP), human chorionic gonadotropin (hCG) and a combination of both hormones (EDP+hCG). TSH-producing cells were labelled using a peroxidase-antiperoxidase immunohistochemical procedure for binding of a rabbit anti-rat beta-thyrotropic polyclonal antisera. Morphometric differences in the cytoplasmic and nuclear volume densities in thyrotropes after the treatments were determined using a stereological method. The relative weights of the pituitary glands were significantly higher in the EDP- and EDP+hCG-treated juvenile and peripubertal rats than in untreated age-matched controls. Treatment with EDP promoted a decrease, and treatment with hCG an increase, of the cellular and nuclear volumes in TSH cells in both juvenile and peripubertal females in comparison with the respective controls. Treatment with a combination of EDP+hCG did not induce any significant changes. The cytoplasmic and nuclear volume densities in TSH cells in the EDP+hCG-treated group were significantly higher than in the EDP-treated, and significantly lower than in hCG-treated rats at both growth stages. These findings suggest that estradiol and hCG exerted opposite effects on pituitary TSH-immunoreactive cells. The observed effects on thyrotrope morphology were apparently independent of the stage of development. (C) 2006 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier GmbH",
journal = "Acta Histochemica",
title = "Immunoreactive TSH cells in juvenile and peripubertal rats after estradiol and human chorionic gonadotropin treatment",
number = "2",
volume = "108",
doi = "10.1016/j.acthis.2006.03.004",
pages = "117-123",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1658"
}

Sekulić, M. I., Šošić-Jurjević, B., Filipović, B., Manojlović-Stojanoski, M.,& Milošević, V.. (2006). Immunoreactive TSH cells in juvenile and peripubertal rats after estradiol and human chorionic gonadotropin treatment. in Acta Histochemica
Elsevier GmbH., 108(2), 117-123.
https://doi.org/10.1016/j.acthis.2006.03.004
https://hdl.handle.net/21.15107/rcub_ibiss_1658

Sekulić MI, Šošić-Jurjević B, Filipović B, Manojlović-Stojanoski M, Milošević V. Immunoreactive TSH cells in juvenile and peripubertal rats after estradiol and human chorionic gonadotropin treatment. in Acta Histochemica. 2006;108(2):117-123.
doi:10.1016/j.acthis.2006.03.004
https://hdl.handle.net/21.15107/rcub_ibiss_1658 .

Sekulić, Milka I., Šošić-Jurjević, Branka , Filipović, Branko, Manojlović-Stojanoski, Milica, Milošević, Verica, "Immunoreactive TSH cells in juvenile and peripubertal rats after estradiol and human chorionic gonadotropin treatment" in Acta Histochemica, 108, no. 2 (2006):117-123,
https://doi.org/10.1016/j.acthis.2006.03.004 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1658 .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1660
UR  - https://link.springer.com/article/10.1007%2Fs00429-005-0057-x
AB  - Elevated glucocorticoid level in the gravid female circulation affects number of endocrine functions in fetuses and offspring. In this research female rats were injected with dexamethasone (Dx) in three consecutive daily doses of 1.0, 0.5, 0.5 mg/kg body weight, starting from day 16 of pregnancy. The influence of this treatment on the pituitary adrenocorticotrophic (ACTH) cells and adrenal glands of 19-day-old fetuses was examined immunocytochemically and by morphometric analysis. Moreover, the proliferative activity of adrenocortical cells was estimated after application of the mitotic inhibitor Oncovine. Administration of Dx to pregnant rats induced a decline of fetal ACTH cell immunopositivity and significant decreases of ACTH cell volume (23%, p < 0.05), volume density (41%, p < 0.05), and its number per unit area (17%, p < 0.05) in comparison to the control 19-day-old fetuses. Reduced proliferative activity of adrenocortical cells (31%; p < 0.05) in zona glomerulosa, as well as the volume of this zone were detected. The volume and number of fetal adrenocortical cells in the inner zone and chromoblasts were not significantly reduced after Dx treatment of pregnant rats. These results show that maternal Dx administration in the period when the fetal hypothalamo-pituitary-adrenal (PA) axis begins its function inhibited the PA axis. Reduced ACTH cell function and mitotic activity led to suppression of adrenocortical cell multiplication in zona glomerulosa, the region of the adrenal cortex where most proliferating cells were found in control 19-day-old fetuses. Thus, increased glucocorticoid levels during late pregnancy caused developmental modifications involving the fetal PA axis, which could be the basis of the altered endocrine responsiveness in adult life.
T2  - Anatomy and Embryology
T1  - The pituitary-adrenal axis of fetal rats after maternal dexamethasone treatment
IS  - 1
VL  - 211
DO  - 10.1007/s00429-005-0057-x
SP  - 61
EP  - 69
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Nestorović, Nataša and Ristić, Nataša and Šošić-Jurjević, Branka  and Filipović, Branko and Milošević, Verica and Sekulić, Milka I.",
year = "2006",
abstract = "Elevated glucocorticoid level in the gravid female circulation affects number of endocrine functions in fetuses and offspring. In this research female rats were injected with dexamethasone (Dx) in three consecutive daily doses of 1.0, 0.5, 0.5 mg/kg body weight, starting from day 16 of pregnancy. The influence of this treatment on the pituitary adrenocorticotrophic (ACTH) cells and adrenal glands of 19-day-old fetuses was examined immunocytochemically and by morphometric analysis. Moreover, the proliferative activity of adrenocortical cells was estimated after application of the mitotic inhibitor Oncovine. Administration of Dx to pregnant rats induced a decline of fetal ACTH cell immunopositivity and significant decreases of ACTH cell volume (23%, p < 0.05), volume density (41%, p < 0.05), and its number per unit area (17%, p < 0.05) in comparison to the control 19-day-old fetuses. Reduced proliferative activity of adrenocortical cells (31%; p < 0.05) in zona glomerulosa, as well as the volume of this zone were detected. The volume and number of fetal adrenocortical cells in the inner zone and chromoblasts were not significantly reduced after Dx treatment of pregnant rats. These results show that maternal Dx administration in the period when the fetal hypothalamo-pituitary-adrenal (PA) axis begins its function inhibited the PA axis. Reduced ACTH cell function and mitotic activity led to suppression of adrenocortical cell multiplication in zona glomerulosa, the region of the adrenal cortex where most proliferating cells were found in control 19-day-old fetuses. Thus, increased glucocorticoid levels during late pregnancy caused developmental modifications involving the fetal PA axis, which could be the basis of the altered endocrine responsiveness in adult life.",
journal = "Anatomy and Embryology",
title = "The pituitary-adrenal axis of fetal rats after maternal dexamethasone treatment",
number = "1",
volume = "211",
doi = "10.1007/s00429-005-0057-x",
pages = "61-69"
}

Manojlović-Stojanoski, M., Nestorović, N., Ristić, N., Šošić-Jurjević, B., Filipović, B., Milošević, V.,& Sekulić, M. I.. (2006). The pituitary-adrenal axis of fetal rats after maternal dexamethasone treatment. in Anatomy and Embryology, 211(1), 61-69.
https://doi.org/10.1007/s00429-005-0057-x

Manojlović-Stojanoski M, Nestorović N, Ristić N, Šošić-Jurjević B, Filipović B, Milošević V, Sekulić MI. The pituitary-adrenal axis of fetal rats after maternal dexamethasone treatment. in Anatomy and Embryology. 2006;211(1):61-69.
doi:10.1007/s00429-005-0057-x .

Manojlović-Stojanoski, Milica, Nestorović, Nataša, Ristić, Nataša, Šošić-Jurjević, Branka , Filipović, Branko, Milošević, Verica, Sekulić, Milka I., "The pituitary-adrenal axis of fetal rats after maternal dexamethasone treatment" in Anatomy and Embryology, 211, no. 1 (2006):61-69,
https://doi.org/10.1007/s00429-005-0057-x . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Sekulić, Milka I.
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1642
AB  - The effects of ovariectomy (Ovx) and of ovariectomy followed by chronic estradiol dipropionate administration (Ovx EDP) on the structure and function of the pituitary-thyroid axis were examined in the rat. Pituitary TSH cells and thyroid tissue were histologically, immunohistochemically and stereologically investigated. Serum TSH and T-4 levels were determined by RIA. Ovx did not affect pituitary weight, but subsequent treatment with EDP led to its more than two-fold increase (p < 0.05). After ovariectomy, the cellular volume of pituitary TSH-immunoreactive cells increased by 28%, p < 0.05 compared to sham-operated animals (SO). Treatment of Ovx rats with EDP partially reversed this change. However, the relative volume density of thyrotrophs decreased in comparison to the Ovx and SO groups (by 18% and 23%, p < 0.05, respectively). No statistically significant differences in serum TSH levels were observed between the experimental groups. In thyroid tissue both peripheral and central follicles responded to Ovx and EDP treatments. Compared to SO rats, the relative volume densities of the follicles and colloid were increased (by 14% and 30%, p < 0.05, respectively) in Ovx rats. Chronic EDP treatment of Ovx rats reversed these changes to the pre-ovariectomy state. Hyperplasia of thyroid follicular cells and a significant reduction (by 21%, p < 0.05) of the serum level of T-4 were detected. In conclusion, estradiol deficiency and chronic treatment affected pituitary TSH cells and thyroid tissue. The sum effect of Ovx on the pituitary-thyroid axis was slightly stimulatory. Subsequent EDP treatment decreased thyroid functioning but at the same time preserved serum TSH at the control level. (c) 2006 Elsevier Inc. All rights reserved.
PB  - Elsevier Inc.
T2  - Life Sciences
T1  - Effects of ovariectomy and chronic estradiol administration on pituitary-thyroid axis in adult rats
IS  - 9
VL  - 79
DO  - 10.1016/j.lfs.2006.03.001
SP  - 890
EP  - 897
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1642
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Filipović, Branko and Milošević, Verica and Nestorović, Nataša and Ristić, Nataša and Sekulić, Milka I.",
year = "2006",
abstract = "The effects of ovariectomy (Ovx) and of ovariectomy followed by chronic estradiol dipropionate administration (Ovx EDP) on the structure and function of the pituitary-thyroid axis were examined in the rat. Pituitary TSH cells and thyroid tissue were histologically, immunohistochemically and stereologically investigated. Serum TSH and T-4 levels were determined by RIA. Ovx did not affect pituitary weight, but subsequent treatment with EDP led to its more than two-fold increase (p < 0.05). After ovariectomy, the cellular volume of pituitary TSH-immunoreactive cells increased by 28%, p < 0.05 compared to sham-operated animals (SO). Treatment of Ovx rats with EDP partially reversed this change. However, the relative volume density of thyrotrophs decreased in comparison to the Ovx and SO groups (by 18% and 23%, p < 0.05, respectively). No statistically significant differences in serum TSH levels were observed between the experimental groups. In thyroid tissue both peripheral and central follicles responded to Ovx and EDP treatments. Compared to SO rats, the relative volume densities of the follicles and colloid were increased (by 14% and 30%, p < 0.05, respectively) in Ovx rats. Chronic EDP treatment of Ovx rats reversed these changes to the pre-ovariectomy state. Hyperplasia of thyroid follicular cells and a significant reduction (by 21%, p < 0.05) of the serum level of T-4 were detected. In conclusion, estradiol deficiency and chronic treatment affected pituitary TSH cells and thyroid tissue. The sum effect of Ovx on the pituitary-thyroid axis was slightly stimulatory. Subsequent EDP treatment decreased thyroid functioning but at the same time preserved serum TSH at the control level. (c) 2006 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Inc.",
journal = "Life Sciences",
title = "Effects of ovariectomy and chronic estradiol administration on pituitary-thyroid axis in adult rats",
number = "9",
volume = "79",
doi = "10.1016/j.lfs.2006.03.001",
pages = "890-897",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1642"
}

Šošić-Jurjević, B., Filipović, B., Milošević, V., Nestorović, N., Ristić, N.,& Sekulić, M. I.. (2006). Effects of ovariectomy and chronic estradiol administration on pituitary-thyroid axis in adult rats. in Life Sciences
Elsevier Inc.., 79(9), 890-897.
https://doi.org/10.1016/j.lfs.2006.03.001
https://hdl.handle.net/21.15107/rcub_ibiss_1642

Šošić-Jurjević B, Filipović B, Milošević V, Nestorović N, Ristić N, Sekulić MI. Effects of ovariectomy and chronic estradiol administration on pituitary-thyroid axis in adult rats. in Life Sciences. 2006;79(9):890-897.
doi:10.1016/j.lfs.2006.03.001
https://hdl.handle.net/21.15107/rcub_ibiss_1642 .

Šošić-Jurjević, Branka , Filipović, Branko, Milošević, Verica, Nestorović, Nataša, Ristić, Nataša, Sekulić, Milka I., "Effects of ovariectomy and chronic estradiol administration on pituitary-thyroid axis in adult rats" in Life Sciences, 79, no. 9 (2006):890-897,
https://doi.org/10.1016/j.lfs.2006.03.001 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1642 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Sekulić, Milka I.
AU  - Starčević, Vesna P.
PY  - 2006
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/234
T2  - Acta biologica serbica - serija C: Acta physiologica et pharmacologica serbica
T1  - Zona glomerulosa cells in juvenile period after treatment with SRIH-14
IS  - 1
VL  - 42
SP  - 57
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_234
ER  - 

@article{
author = "Milošević, Verica and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Sekulić, Milka I. and Starčević, Vesna P.",
year = "2006, 2006",
journal = "Acta biologica serbica - serija C: Acta physiologica et pharmacologica serbica",
title = "Zona glomerulosa cells in juvenile period after treatment with SRIH-14",
number = "1",
volume = "42",
pages = "57-60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_234"
}

Milošević, V., Nestorović, N., Manojlović-Stojanoski, M., Sekulić, M. I.,& Starčević, V. P.. (2006). Zona glomerulosa cells in juvenile period after treatment with SRIH-14. in Acta biologica serbica - serija C: Acta physiologica et pharmacologica serbica, 42(1), 57-60.
https://hdl.handle.net/21.15107/rcub_ibiss_234

Milošević V, Nestorović N, Manojlović-Stojanoski M, Sekulić MI, Starčević VP. Zona glomerulosa cells in juvenile period after treatment with SRIH-14. in Acta biologica serbica - serija C: Acta physiologica et pharmacologica serbica. 2006;42(1):57-60.
https://hdl.handle.net/21.15107/rcub_ibiss_234 .

Milošević, Verica, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Sekulić, Milka I., Starčević, Vesna P., "Zona glomerulosa cells in juvenile period after treatment with SRIH-14" in Acta biologica serbica - serija C: Acta physiologica et pharmacologica serbica, 42, no. 1 (2006):57-60,
https://hdl.handle.net/21.15107/rcub_ibiss_234 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Nestorović, Nataša
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Manojlović-Stojanoski, Milica
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1717
AB  - Structural and morphometric features of thyroid C and follicularcells were studied in adult rat females after treatment with synthetic salmon calcitonin (CT). The animals were chronically treated with either a low (10 IU/kg b.w) or a high (100 IU/kg b.w) dose of CT. A stereological method was applied to determine the volume density and the number of immunoreactive C cells. The height and volume density of follicular epithelium, colloid, interstitium and the follicles (epithelium plus colloid), as well as the index of activation rate were calculated. A significant decrease in body weight, as well as the volume density of immunoreactive C cells and the number of C cells per mm(2), was observed in rats treated with both doses of CT. The height and volume density of follicular epithelium and follicles, as well as the index of activation rate were significantly increased in the animals given the high CT dose, while the volume densities of colloid and interstitium were reduced. No significant changes in the examined morphometric parameters were detected after treatment with the low CT dose. According to these results it can be concluded that the structural features of thyroid C and follicular cells were affected by the high dose CT treatment in the opposite manner, while the low dose CT treatment influenced only C cells.
PB  - Polish Histochemical and Cytochemical Society
T2  - Folia Histochemica et Cytobiologica
T1  - The effects of synthetic salmon calcitonin on thyroid C and follicular cells in adult female rats
IS  - 2
VL  - 43
SP  - 103
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1717
ER  - 

@article{
author = "Sekulić, Milka I. and Nestorović, Nataša and Šošić-Jurjević, Branka  and Filipović, Branko and Milošević, Verica and Manojlović-Stojanoski, Milica",
year = "2005",
abstract = "Structural and morphometric features of thyroid C and follicularcells were studied in adult rat females after treatment with synthetic salmon calcitonin (CT). The animals were chronically treated with either a low (10 IU/kg b.w) or a high (100 IU/kg b.w) dose of CT. A stereological method was applied to determine the volume density and the number of immunoreactive C cells. The height and volume density of follicular epithelium, colloid, interstitium and the follicles (epithelium plus colloid), as well as the index of activation rate were calculated. A significant decrease in body weight, as well as the volume density of immunoreactive C cells and the number of C cells per mm(2), was observed in rats treated with both doses of CT. The height and volume density of follicular epithelium and follicles, as well as the index of activation rate were significantly increased in the animals given the high CT dose, while the volume densities of colloid and interstitium were reduced. No significant changes in the examined morphometric parameters were detected after treatment with the low CT dose. According to these results it can be concluded that the structural features of thyroid C and follicular cells were affected by the high dose CT treatment in the opposite manner, while the low dose CT treatment influenced only C cells.",
publisher = "Polish Histochemical and Cytochemical Society",
journal = "Folia Histochemica et Cytobiologica",
title = "The effects of synthetic salmon calcitonin on thyroid C and follicular cells in adult female rats",
number = "2",
volume = "43",
pages = "103-108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1717"
}

Sekulić, M. I., Nestorović, N., Šošić-Jurjević, B., Filipović, B., Milošević, V.,& Manojlović-Stojanoski, M.. (2005). The effects of synthetic salmon calcitonin on thyroid C and follicular cells in adult female rats. in Folia Histochemica et Cytobiologica
Polish Histochemical and Cytochemical Society., 43(2), 103-108.
https://hdl.handle.net/21.15107/rcub_ibiss_1717

Sekulić MI, Nestorović N, Šošić-Jurjević B, Filipović B, Milošević V, Manojlović-Stojanoski M. The effects of synthetic salmon calcitonin on thyroid C and follicular cells in adult female rats. in Folia Histochemica et Cytobiologica. 2005;43(2):103-108.
https://hdl.handle.net/21.15107/rcub_ibiss_1717 .

Sekulić, Milka I., Nestorović, Nataša, Šošić-Jurjević, Branka , Filipović, Branko, Milošević, Verica, Manojlović-Stojanoski, Milica, "The effects of synthetic salmon calcitonin on thyroid C and follicular cells in adult female rats" in Folia Histochemica et Cytobiologica, 43, no. 2 (2005):103-108,
https://hdl.handle.net/21.15107/rcub_ibiss_1717 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Trifunović, Svetlana
AU  - Sekulić, Milka I.
AU  - Šošić-Jurjević, Branka 
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Starčević, Vesna P.
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1690
AB  - The effect of chronic exposure to light of adult Wistar rats on growth and function of adrenal zona glomerulosa (ZG) and zona fasciculata (ZF) were examined. The females were exposed to continuous light of 600 lux for 95 days, starting on day 30 of age. The controls were kept under a 12 : 12 h light-dark cycle, at ambient temperature. The rats were sacrificed by decapitation and the left adrenal gland of each animal was dissected out and prepared for morphometric analyses. In animals exposed to chronic lighting, the absolute and relative volume of ZG were insignificantly increased by 5% (p > 0.05) compared to controls. The volume of ZG cells and their nuclei were insignificantly changed by 1% (p > 0.05) in comparison with corresponding controls. The absolute and relative volume of ZF were significantly increased (by 14 and 9%, respectively, p < 0.05), as compared to controls. The volume of ZF cells and their nuclei were significantly increased (by 12 and 9%, respectively; p < 0.05). Serum concentration of corticosterone was also significantly (p < 0.05) increased by 13 % in light-exposed group in comparison with control rats. These findings suggest that continuous exposure of female rats to constant light increased growth and secretory activity of ZF cells.
PB  - Slovak Academy of Sciences, Institute of Molecular Physiology and Genetics
T2  - General Physiology and Biophysics
T1  - Chronic exposure to constant light affects morphology and secretion of adrenal zona fasciculata cells in female rats
IS  - 3
VL  - 24
SP  - 299
EP  - 309
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1690
ER  - 

@article{
author = "Milošević, Verica and Trifunović, Svetlana and Sekulić, Milka I. and Šošić-Jurjević, Branka  and Ristić, Nataša and Filipović, Branko and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Starčević, Vesna P.",
year = "2005",
abstract = "The effect of chronic exposure to light of adult Wistar rats on growth and function of adrenal zona glomerulosa (ZG) and zona fasciculata (ZF) were examined. The females were exposed to continuous light of 600 lux for 95 days, starting on day 30 of age. The controls were kept under a 12 : 12 h light-dark cycle, at ambient temperature. The rats were sacrificed by decapitation and the left adrenal gland of each animal was dissected out and prepared for morphometric analyses. In animals exposed to chronic lighting, the absolute and relative volume of ZG were insignificantly increased by 5% (p > 0.05) compared to controls. The volume of ZG cells and their nuclei were insignificantly changed by 1% (p > 0.05) in comparison with corresponding controls. The absolute and relative volume of ZF were significantly increased (by 14 and 9%, respectively, p < 0.05), as compared to controls. The volume of ZF cells and their nuclei were significantly increased (by 12 and 9%, respectively; p < 0.05). Serum concentration of corticosterone was also significantly (p < 0.05) increased by 13 % in light-exposed group in comparison with control rats. These findings suggest that continuous exposure of female rats to constant light increased growth and secretory activity of ZF cells.",
publisher = "Slovak Academy of Sciences, Institute of Molecular Physiology and Genetics",
journal = "General Physiology and Biophysics",
title = "Chronic exposure to constant light affects morphology and secretion of adrenal zona fasciculata cells in female rats",
number = "3",
volume = "24",
pages = "299-309",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1690"
}

Milošević, V., Trifunović, S., Sekulić, M. I., Šošić-Jurjević, B., Ristić, N., Filipović, B., Nestorović, N., Manojlović-Stojanoski, M.,& Starčević, V. P.. (2005). Chronic exposure to constant light affects morphology and secretion of adrenal zona fasciculata cells in female rats. in General Physiology and Biophysics
Slovak Academy of Sciences, Institute of Molecular Physiology and Genetics., 24(3), 299-309.
https://hdl.handle.net/21.15107/rcub_ibiss_1690

Milošević V, Trifunović S, Sekulić MI, Šošić-Jurjević B, Ristić N, Filipović B, Nestorović N, Manojlović-Stojanoski M, Starčević VP. Chronic exposure to constant light affects morphology and secretion of adrenal zona fasciculata cells in female rats. in General Physiology and Biophysics. 2005;24(3):299-309.
https://hdl.handle.net/21.15107/rcub_ibiss_1690 .

Milošević, Verica, Trifunović, Svetlana, Sekulić, Milka I., Šošić-Jurjević, Branka , Ristić, Nataša, Filipović, Branko, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Starčević, Vesna P., "Chronic exposure to constant light affects morphology and secretion of adrenal zona fasciculata cells in female rats" in General Physiology and Biophysics, 24, no. 3 (2005):299-309,
https://hdl.handle.net/21.15107/rcub_ibiss_1690 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka 
AU  - Bujšić, Nada
AU  - Sekulić, Milka I.
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/469
AB  - The effects of multiple doses of estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) on the growth and function of pituitary somatotropes (GH cells) were studied. Female middle-aged rats were receiving i.p. EDP (0.625 mg i.p./kg b.w), or Ca (11.4 mg/kg b.w) every day for two weeks. Blood samples were collected for hormone analyses and pituitaries dissected for histological and morphometric evaluation 24 h after the last injection. GH-producing cells were examined using the peroxidase-antiperoxidase (PAP) immuno-histochemical procedure. Both EDP- and Ca-treatment significantly decreased all morphometric parameters of GH cells (p<0.05) in comparison with the corresponding controls. Serum concentration of growth hormone (GH) in EDP- or Ca-treated groups was lower by 65% and 13% (p<0.05) respectively comparing to the controls. The difference between all morphometric parameters of EDP- and Ca-treated rats was statistically significant (p<0.05) in relation to the controls. These findings suggest that multiple EDP, or Ca application affects (directly or indirectly) the control of growth and secretory activity of GH cells in middle-aged female rats.
AB  - Ispitivani su efekti višekratnih doza estradiol dipropionata (EDP) ili kalcijum glukoheptonata (Ca) na rast i funkciju hipofiznih ćelija hormona rasta (GH) acikličnih ženki Wistar pacova. Aciklične ženke su svakodnevno tokom dve nedelje i.p. dobijale 0.625 mg EDP/kg t.m., ili 11.4 mg Ca/kg t.m.. Krv je sakuplja na radi određivanja koncentracije hormona rasta u serumu, a hipofize su pripremane za histološku i morfometrijsku analizu. GH ćelije su imunocitohemijski obeležavane metodom peroksidaza-antiperoksidaza (PAP). Morfometrijski para-metri su bili značajno smanjeni (p<0.05) u životinja tretiranih EDP-om, ili kalcijumom u poređenju sa odgovarajućim kontrolama. Koncentracija hormona rasta u serumu bila je takođe značajno niža (p<0.05) u obe tretirane grupe (za 65% odnosno za 13%) u poređenju sa odgovarajućom kontrolom. Dobijeni rezultati ukazuju da višekratni tretman acikličnih ženki pacova EDP-om ili Ca-glukoheptonatom deluje inhibitorno na GH ćelije i da su i estrogenui i kalcijum uključeni na specifičan način u rast i sekretornu aktivnost GH ćelija hipofize.
PB  - Belgrade: University of Belgrade - Faculty of Veterinary Medicine
T2  - Acta veterinaria
T1  - Delovanje estradiola i kalcijuma na ćelije hipofize koje luče hormon rasta u acikličnih ženki pacova
T1  - Estradiol and calcium affect the growth hormone producing cells in female middle-aged rats
IS  - 2-3
VL  - 55
DO  - 10.2298/AVB0503111M
SP  - 111
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_469
ER  - 

@article{
author = "Milošević, Verica and Trifunović, Svetlana and Šošić-Jurjević, Branka  and Bujšić, Nada and Sekulić, Milka I.",
year = "2005, 2005",
abstract = "The effects of multiple doses of estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) on the growth and function of pituitary somatotropes (GH cells) were studied. Female middle-aged rats were receiving i.p. EDP (0.625 mg i.p./kg b.w), or Ca (11.4 mg/kg b.w) every day for two weeks. Blood samples were collected for hormone analyses and pituitaries dissected for histological and morphometric evaluation 24 h after the last injection. GH-producing cells were examined using the peroxidase-antiperoxidase (PAP) immuno-histochemical procedure. Both EDP- and Ca-treatment significantly decreased all morphometric parameters of GH cells (p<0.05) in comparison with the corresponding controls. Serum concentration of growth hormone (GH) in EDP- or Ca-treated groups was lower by 65% and 13% (p<0.05) respectively comparing to the controls. The difference between all morphometric parameters of EDP- and Ca-treated rats was statistically significant (p<0.05) in relation to the controls. These findings suggest that multiple EDP, or Ca application affects (directly or indirectly) the control of growth and secretory activity of GH cells in middle-aged female rats., Ispitivani su efekti višekratnih doza estradiol dipropionata (EDP) ili kalcijum glukoheptonata (Ca) na rast i funkciju hipofiznih ćelija hormona rasta (GH) acikličnih ženki Wistar pacova. Aciklične ženke su svakodnevno tokom dve nedelje i.p. dobijale 0.625 mg EDP/kg t.m., ili 11.4 mg Ca/kg t.m.. Krv je sakuplja na radi određivanja koncentracije hormona rasta u serumu, a hipofize su pripremane za histološku i morfometrijsku analizu. GH ćelije su imunocitohemijski obeležavane metodom peroksidaza-antiperoksidaza (PAP). Morfometrijski para-metri su bili značajno smanjeni (p<0.05) u životinja tretiranih EDP-om, ili kalcijumom u poređenju sa odgovarajućim kontrolama. Koncentracija hormona rasta u serumu bila je takođe značajno niža (p<0.05) u obe tretirane grupe (za 65% odnosno za 13%) u poređenju sa odgovarajućom kontrolom. Dobijeni rezultati ukazuju da višekratni tretman acikličnih ženki pacova EDP-om ili Ca-glukoheptonatom deluje inhibitorno na GH ćelije i da su i estrogenui i kalcijum uključeni na specifičan način u rast i sekretornu aktivnost GH ćelija hipofize.",
publisher = "Belgrade: University of Belgrade - Faculty of Veterinary Medicine",
journal = "Acta veterinaria",
title = "Delovanje estradiola i kalcijuma na ćelije hipofize koje luče hormon rasta u acikličnih ženki pacova, Estradiol and calcium affect the growth hormone producing cells in female middle-aged rats",
number = "2-3",
volume = "55",
doi = "10.2298/AVB0503111M",
pages = "111-120",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_469"
}

Milošević, V., Trifunović, S., Šošić-Jurjević, B., Bujšić, N.,& Sekulić, M. I.. (2005). Delovanje estradiola i kalcijuma na ćelije hipofize koje luče hormon rasta u acikličnih ženki pacova. in Acta veterinaria
Belgrade: University of Belgrade - Faculty of Veterinary Medicine., 55(2-3), 111-120.
https://doi.org/10.2298/AVB0503111M
https://hdl.handle.net/21.15107/rcub_ibiss_469

Milošević V, Trifunović S, Šošić-Jurjević B, Bujšić N, Sekulić MI. Delovanje estradiola i kalcijuma na ćelije hipofize koje luče hormon rasta u acikličnih ženki pacova. in Acta veterinaria. 2005;55(2-3):111-120.
doi:10.2298/AVB0503111M
https://hdl.handle.net/21.15107/rcub_ibiss_469 .

Milošević, Verica, Trifunović, Svetlana, Šošić-Jurjević, Branka , Bujšić, Nada, Sekulić, Milka I., "Delovanje estradiola i kalcijuma na ćelije hipofize koje luče hormon rasta u acikličnih ženki pacova" in Acta veterinaria, 55, no. 2-3 (2005):111-120,
https://doi.org/10.2298/AVB0503111M .,
https://hdl.handle.net/21.15107/rcub_ibiss_469 .

TY  - JOUR
AU  - Nestorović, Nataša
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Ristić, Nataša
AU  - Šošić-Jurjević, Branka 
AU  - Manojlović-Stojanoski, Milica
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/32
AB  - Effects of intracerbroventricularly (ICV) administered octreotide on gonadotrophic cells (FSH and LH) of adult Wistar female rats were examined by immunocytochemical and morphometric methods. The animals received ICV three 1.0 mg doses of octreotide dissolved in 10 mL saline every second day. The controls were treated with equivalent volume of physiological saline by the same schedule. FSH- and LH-producing cells were examined using peroxidase-antiperoxidase immunocytochemical procedure. Morphometric and stereologic examinations were performed to evaluate changes in the number, volume and volume densities of gonadotrophic cells. In females treated with octreotide, the gonadotrophic cells were smaller and often pycnotic, while the number of FSH- and LH-immunopositive cells per unit area (mm2) was significantly reduced. Octreotide also induced a significant reduction of the FSH- and LH-immunoreactive cells volume, as well as of their volume densities. On the basis of these results it can be concluded that octeotride, centrally administered to adult female rats provokes changes in immunocytochemical and morphometric features of both types of gonadotrophic cells.
AB  - Ispitivani su efekti oktreotida davanog intracerebroventrikularno (ICV) odraslim ženkama pacova na gonadotropne ćelije (FSH i LH). Životinje su tretirane sa ukupno tri doze od 1 mg oktreotida rastvorenog u 10 mL fiziološkog rastvora svakog drugog dana. Kontrole su na isti način primile odgovarajuću zapreminu fiziološ kog rastvora. FSH i LH ćelije su ispitivane imunocitohemijskim postupkom peroksidaza-antiperoksidaza. Da bi se ustanovile promene u broju, zapremini i zapreminskoj gustini gonadotropnih ćelija primenjen je morfometrijski kao i stereološki pristup. Kod ženki tretiranih oktreotidom, gonadotropne ćelije su bile manje i često piknotič ne. Broj imunopozitivnih FSH i LH ćelija po jedinici površine (mm2) je bio statistički značajno smanjen. Oktreotid je, takodje, izazvao značajno smanjenje zapremine FSH i LH imunoreaktivnih ćelija, kao i njihove zapreminske gustine. Na osnovu ovih rezultata, može se zaključiti da je oktreotid, dat ICV odraslim ženkama pacova doveo do promena imunocitohemijskih i morfometrijskih osobina oba ispitivana tipa gonadotropnih ćelija.
PB  - Society of Medical Biochemists of Serbia
T2  - Jugoslovenska medicinska biohemija
T1  - Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova
T1  - Effects of intracerebroventricularly administered octreotide on gonadotrophic cells in female rats
IS  - 2
VL  - 23
DO  - 10.2298/JMH0402149N
SP  - 149
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_32
ER  - 

@article{
author = "Nestorović, Nataša and Lovren, Mirjana B. and Sekulić, Milka I. and Ristić, Nataša and Šošić-Jurjević, Branka  and Manojlović-Stojanoski, Milica and Filipović, Branko and Milošević, Verica",
year = "2004",
abstract = "Effects of intracerbroventricularly (ICV) administered octreotide on gonadotrophic cells (FSH and LH) of adult Wistar female rats were examined by immunocytochemical and morphometric methods. The animals received ICV three 1.0 mg doses of octreotide dissolved in 10 mL saline every second day. The controls were treated with equivalent volume of physiological saline by the same schedule. FSH- and LH-producing cells were examined using peroxidase-antiperoxidase immunocytochemical procedure. Morphometric and stereologic examinations were performed to evaluate changes in the number, volume and volume densities of gonadotrophic cells. In females treated with octreotide, the gonadotrophic cells were smaller and often pycnotic, while the number of FSH- and LH-immunopositive cells per unit area (mm2) was significantly reduced. Octreotide also induced a significant reduction of the FSH- and LH-immunoreactive cells volume, as well as of their volume densities. On the basis of these results it can be concluded that octeotride, centrally administered to adult female rats provokes changes in immunocytochemical and morphometric features of both types of gonadotrophic cells., Ispitivani su efekti oktreotida davanog intracerebroventrikularno (ICV) odraslim ženkama pacova na gonadotropne ćelije (FSH i LH). Životinje su tretirane sa ukupno tri doze od 1 mg oktreotida rastvorenog u 10 mL fiziološkog rastvora svakog drugog dana. Kontrole su na isti način primile odgovarajuću zapreminu fiziološ kog rastvora. FSH i LH ćelije su ispitivane imunocitohemijskim postupkom peroksidaza-antiperoksidaza. Da bi se ustanovile promene u broju, zapremini i zapreminskoj gustini gonadotropnih ćelija primenjen je morfometrijski kao i stereološki pristup. Kod ženki tretiranih oktreotidom, gonadotropne ćelije su bile manje i često piknotič ne. Broj imunopozitivnih FSH i LH ćelija po jedinici površine (mm2) je bio statistički značajno smanjen. Oktreotid je, takodje, izazvao značajno smanjenje zapremine FSH i LH imunoreaktivnih ćelija, kao i njihove zapreminske gustine. Na osnovu ovih rezultata, može se zaključiti da je oktreotid, dat ICV odraslim ženkama pacova doveo do promena imunocitohemijskih i morfometrijskih osobina oba ispitivana tipa gonadotropnih ćelija.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Jugoslovenska medicinska biohemija",
title = "Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova, Effects of intracerebroventricularly administered octreotide on gonadotrophic cells in female rats",
number = "2",
volume = "23",
doi = "10.2298/JMH0402149N",
pages = "149-153",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_32"
}

Nestorović, N., Lovren, M. B., Sekulić, M. I., Ristić, N., Šošić-Jurjević, B., Manojlović-Stojanoski, M., Filipović, B.,& Milošević, V.. (2004). Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova. in Jugoslovenska medicinska biohemija
Society of Medical Biochemists of Serbia., 23(2), 149-153.
https://doi.org/10.2298/JMH0402149N
https://hdl.handle.net/21.15107/rcub_ibiss_32

Nestorović N, Lovren MB, Sekulić MI, Ristić N, Šošić-Jurjević B, Manojlović-Stojanoski M, Filipović B, Milošević V. Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova. in Jugoslovenska medicinska biohemija. 2004;23(2):149-153.
doi:10.2298/JMH0402149N
https://hdl.handle.net/21.15107/rcub_ibiss_32 .

Nestorović, Nataša, Lovren, Mirjana B., Sekulić, Milka I., Ristić, Nataša, Šošić-Jurjević, Branka , Manojlović-Stojanoski, Milica, Filipović, Branko, Milošević, Verica, "Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova" in Jugoslovenska medicinska biohemija, 23, no. 2 (2004):149-153,
https://doi.org/10.2298/JMH0402149N .,
https://hdl.handle.net/21.15107/rcub_ibiss_32 .

TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Kostić, Nada D
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1761
AB  - The structure and function of thyroid C cells were studied in ovariectomized (Ovx) adult female rats without and after chronic treatment with estradiol dipropionate (EDP). A peroxidase-antiperoxidase method was applied for localization of calcitonin (CT) in the C cells. Morphometric changes in their volume, nuclei, and relative volume density were evaluated in comparison with sham-operated control rats using a stereological method. The number of C cells was calculated. CT content in the sera was determined by radioimmunoassay. Ovariectomy (Ovx) led to a 21% increase in body weight (P<0.005), while treatment of Ovx rats with EDP decreased body weight by 25% (P<0.01). The immunoreactivity for CT in C cells of the Ovx rats was markedly increased. Significant decreases in the volume of C cells (by 13%; P<0.05) and serum CT (by 45%) were recorded, while the C cell number increased by 59% (P<0.05) in relation to the corresponding controls. The treatment of Ovx rats with EDP caused conspicuous degranulation of the C cells. The cellular volume was increased by 11% and serum CT by 36% in comparison with Ovx animals. At the same time a decrease in C cell number by 29% (P<0.05) was evident. It may be concluded that estradiol deficiency after Ovx reduced the synthesis and release of CT, while chronic treatment of these animals with EDP had a positive effect on the secretory activity of thyroid C cells.
PB  - Springer-Verlag
T2  - Histochemistry and Cell Biology
T1  - The thyroid C cells of ovariectomized rats treated with estradiol
IS  - 5
VL  - 120
DO  - 10.1007/s00418-003-0578-4
SP  - 409
EP  - 414
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1761
ER  - 

@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka  and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Kostić, Nada D and Milošević, Verica and Sekulić, Milka I.",
year = "2003",
abstract = "The structure and function of thyroid C cells were studied in ovariectomized (Ovx) adult female rats without and after chronic treatment with estradiol dipropionate (EDP). A peroxidase-antiperoxidase method was applied for localization of calcitonin (CT) in the C cells. Morphometric changes in their volume, nuclei, and relative volume density were evaluated in comparison with sham-operated control rats using a stereological method. The number of C cells was calculated. CT content in the sera was determined by radioimmunoassay. Ovariectomy (Ovx) led to a 21% increase in body weight (P<0.005), while treatment of Ovx rats with EDP decreased body weight by 25% (P<0.01). The immunoreactivity for CT in C cells of the Ovx rats was markedly increased. Significant decreases in the volume of C cells (by 13%; P<0.05) and serum CT (by 45%) were recorded, while the C cell number increased by 59% (P<0.05) in relation to the corresponding controls. The treatment of Ovx rats with EDP caused conspicuous degranulation of the C cells. The cellular volume was increased by 11% and serum CT by 36% in comparison with Ovx animals. At the same time a decrease in C cell number by 29% (P<0.05) was evident. It may be concluded that estradiol deficiency after Ovx reduced the synthesis and release of CT, while chronic treatment of these animals with EDP had a positive effect on the secretory activity of thyroid C cells.",
publisher = "Springer-Verlag",
journal = "Histochemistry and Cell Biology",
title = "The thyroid C cells of ovariectomized rats treated with estradiol",
number = "5",
volume = "120",
doi = "10.1007/s00418-003-0578-4",
pages = "409-414",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1761"
}

Filipović, B., Šošić-Jurjević, B., Nestorović, N., Manojlović-Stojanoski, M., Kostić, N. D., Milošević, V.,& Sekulić, M. I.. (2003). The thyroid C cells of ovariectomized rats treated with estradiol. in Histochemistry and Cell Biology
Springer-Verlag., 120(5), 409-414.
https://doi.org/10.1007/s00418-003-0578-4
https://hdl.handle.net/21.15107/rcub_ibiss_1761

Filipović B, Šošić-Jurjević B, Nestorović N, Manojlović-Stojanoski M, Kostić ND, Milošević V, Sekulić MI. The thyroid C cells of ovariectomized rats treated with estradiol. in Histochemistry and Cell Biology. 2003;120(5):409-414.
doi:10.1007/s00418-003-0578-4
https://hdl.handle.net/21.15107/rcub_ibiss_1761 .

Filipović, Branko, Šošić-Jurjević, Branka , Nestorović, Nataša, Manojlović-Stojanoski, Milica, Kostić, Nada D, Milošević, Verica, Sekulić, Milka I., "The thyroid C cells of ovariectomized rats treated with estradiol" in Histochemistry and Cell Biology, 120, no. 5 (2003):409-414,
https://doi.org/10.1007/s00418-003-0578-4 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1761 .