TY  - CONF
AU  - Tubić, Ljiljana
AU  - Lazić, Dejan
AU  - Petrović, Nikola
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6450
AB  - Postojanje zelenih krovova značajno ublažava efekat urbanog ostrva toplote, 
pozitivno utiče na kvalitet vazduha, a ujedno smanjuje količinu energije koja se potroši 
za hlađenje i grejanje objekta. Kasetni zeleni krov 7,5 projektovan od strane tima Green 
decor doo uspešno i brzo pretvara sivi krov u funkcionalni zeleni prostor. Ovakvi 
ekstenzivni zeleni krovovi su posebno pogodni za ravne krovove, ali i za kose koji su pod 
uglom većim od 15 stepeni. Stabilne i lako uklopljive kasete su napravljene od recikliranih 
materijala. Njihov drenažno-akumulacioni sloj pokazuje dodatnu važnost tokom 
ekstremnih vremenskih uslova sa kojima se susrećemo poslednjih godina u Beogradu. 
Značaj zelenih krovova uvek treba posmatrati u odnosu na širu zajednicu i njeno 
okruženje. Pored svih ovih prednosti, zeleni krovovi u našim gradovima još uvek nisu 
dovoljno prepoznati i podržani. Zbog toga je neophodno sprovesti naučna istraživanja 
koja će nam pomoći da pokažemo da su kasetni zeleni krovovi važan faktor za formiranje 
zdravog grada. Uslovi na krovovima često mogu biti pravi izazov za život biljaka, stoga 
je potrebno posebnu pažnju posvetiti odabiru biljaka za zeleni krov. Ujedno moramo 
razmišljati da će zeleni krovovi biti pogodna staništa i za druge organizme (insekti, ptice, 
mahovine, lišajevi, mikroorganizmi u supstratu). Sve ovo nas dovodi do zaključka da 
pravilno dizajniran kasetni zeleni krov u velikoj meri doprinosi očuvanju biodiverziteta 
u gradskim uslovima.
PB  - Belgrade: Faculty of Forestry, University of Belgrade
C3  - Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia
T1  - Kasetni zeleni krovovi za zdrav grad
SP  - 321
EP  - 330
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6450
ER  - 

@conference{
author = "Tubić, Ljiljana and Lazić, Dejan and Petrović, Nikola",
year = "2023",
abstract = "Postojanje zelenih krovova značajno ublažava efekat urbanog ostrva toplote, 
pozitivno utiče na kvalitet vazduha, a ujedno smanjuje količinu energije koja se potroši 
za hlađenje i grejanje objekta. Kasetni zeleni krov 7,5 projektovan od strane tima Green 
decor doo uspešno i brzo pretvara sivi krov u funkcionalni zeleni prostor. Ovakvi 
ekstenzivni zeleni krovovi su posebno pogodni za ravne krovove, ali i za kose koji su pod 
uglom većim od 15 stepeni. Stabilne i lako uklopljive kasete su napravljene od recikliranih 
materijala. Njihov drenažno-akumulacioni sloj pokazuje dodatnu važnost tokom 
ekstremnih vremenskih uslova sa kojima se susrećemo poslednjih godina u Beogradu. 
Značaj zelenih krovova uvek treba posmatrati u odnosu na širu zajednicu i njeno 
okruženje. Pored svih ovih prednosti, zeleni krovovi u našim gradovima još uvek nisu 
dovoljno prepoznati i podržani. Zbog toga je neophodno sprovesti naučna istraživanja 
koja će nam pomoći da pokažemo da su kasetni zeleni krovovi važan faktor za formiranje 
zdravog grada. Uslovi na krovovima često mogu biti pravi izazov za život biljaka, stoga 
je potrebno posebnu pažnju posvetiti odabiru biljaka za zeleni krov. Ujedno moramo 
razmišljati da će zeleni krovovi biti pogodna staništa i za druge organizme (insekti, ptice, 
mahovine, lišajevi, mikroorganizmi u supstratu). Sve ovo nas dovodi do zaključka da 
pravilno dizajniran kasetni zeleni krov u velikoj meri doprinosi očuvanju biodiverziteta 
u gradskim uslovima.",
publisher = "Belgrade: Faculty of Forestry, University of Belgrade",
journal = "Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia",
title = "Kasetni zeleni krovovi za zdrav grad",
pages = "321-330",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6450"
}

Tubić, L., Lazić, D.,& Petrović, N.. (2023). Kasetni zeleni krovovi za zdrav grad. in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia
Belgrade: Faculty of Forestry, University of Belgrade., 321-330.
https://hdl.handle.net/21.15107/rcub_ibiss_6450

Tubić L, Lazić D, Petrović N. Kasetni zeleni krovovi za zdrav grad. in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia. 2023;:321-330.
https://hdl.handle.net/21.15107/rcub_ibiss_6450 .

Tubić, Ljiljana, Lazić, Dejan, Petrović, Nikola, "Kasetni zeleni krovovi za zdrav grad" in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia (2023):321-330,
https://hdl.handle.net/21.15107/rcub_ibiss_6450 .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6372
AB  - French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.
PB  - Springer Nature
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil
DO  - 10.1007/s00344-023-11131-8
ER  - 

@article{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in
fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil
(EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the
first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods
(4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered
phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–
glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins,
storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed.
In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time
exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes
involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing
of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the
highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like)
exhibiting the highest expression rate.",
publisher = "Springer Nature",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil",
doi = "10.1007/s00344-023-11131-8"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation
Springer Nature..
https://doi.org/10.1007/s00344-023-11131-8

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil. in Journal of Plant Growth Regulation. 2023;.
doi:10.1007/s00344-023-11131-8 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of Potato (Solanum tuberosum L.) Exposed to French Marigold (Tagetes patula L.) Essential Oil" in Journal of Plant Growth Regulation (2023),
https://doi.org/10.1007/s00344-023-11131-8 . .

TY  - DATA
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5388
AB  - Flower strips of French marigold are commonly used pest repellents in potato fields. However, the effect of French marigold volatiles on potato metabolism is unknown. Since it is shown that different volatiles affect phytohormone signaling pathways, it is assumed that phytohormones have an important role in response to essential oils. Thus, a microarray transcriptome analysis and annotation of differentialy expresed transcripts into different phytohormone metabolic pathways was performed to study the effects of French marigold essential oil on laboratory-grown potato phytohormone homeostasis. Obtained research data are presented in 4 csv files. Names of the files are abbreviated names of corresponding phytohormone groups. Supporting information about the data are presented in the Read Me file.
T1  - Microarray data of phytohormone related genes in potato exposed to French marigold essential oil
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5388
ER  - 

@misc{
author = "Stupar, Sofija and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2023",
abstract = "Flower strips of French marigold are commonly used pest repellents in potato fields. However, the effect of French marigold volatiles on potato metabolism is unknown. Since it is shown that different volatiles affect phytohormone signaling pathways, it is assumed that phytohormones have an important role in response to essential oils. Thus, a microarray transcriptome analysis and annotation of differentialy expresed transcripts into different phytohormone metabolic pathways was performed to study the effects of French marigold essential oil on laboratory-grown potato phytohormone homeostasis. Obtained research data are presented in 4 csv files. Names of the files are abbreviated names of corresponding phytohormone groups. Supporting information about the data are presented in the Read Me file.",
title = "Microarray data of phytohormone related genes in potato exposed to French marigold essential oil",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5388"
}

Stupar, S., Devrnja, N., Tubić, L.,& Savić, J.. (2023). Microarray data of phytohormone related genes in potato exposed to French marigold essential oil. .
https://hdl.handle.net/21.15107/rcub_ibiss_5388

Stupar S, Devrnja N, Tubić L, Savić J. Microarray data of phytohormone related genes in potato exposed to French marigold essential oil. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5388 .

Stupar, Sofija, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Microarray data of phytohormone related genes in potato exposed to French marigold essential oil" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5388 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Motyka, Vaclav
AU  - Dobrev, Petre I.
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5049
AB  - French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
T1  - Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5049
ER  - 

@conference{
author = "Stupar, Sofija and Motyka, Vaclav and Dobrev, Petre I. and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022",
title = "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5049"
}

Stupar, S., Motyka, V., Dobrev, P. I., Ćosić, T., Devrnja, N., Tubić, L.,& Savić, J.. (2022). Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022
Belgrade: Serbian Plant Physiology Society., 76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049

Stupar S, Motyka V, Dobrev PI, Ćosić T, Devrnja N, Tubić L, Savić J. Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil. in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022. 2022;:76.
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

Stupar, Sofija, Motyka, Vaclav, Dobrev, Petre I., Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Savić, Jelena, "Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil" in Book of Abstracts / 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade; organized by Serbian Plant Physiology Society and Institute for Biological Research “Siniša Stanković”, University of Belgrade and Faculty of Biology, University of Belgrade ; editor Milica Milutinović, Belgrade, 2022 (2022):76,
https://hdl.handle.net/21.15107/rcub_ibiss_5049 .

TY  - CONF
AU  - Ninković, Nina
AU  - Tubić, Ljiljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6493
AB  - Ekstrakt listova vlašca je bogat flavonolima (kemferol, kvercetin i izoramnetin) i flavonol nim glikozidima (kemferol-3-O-glikozid i kvercetin-3-O-glikozid). Ova jedinjenja predstavljaju važnu grupu flanovoida sa širokim spektrom biološke aktivnosti. Cilj ovog istraživanja je da se ispita kako klijanci model biljke A. thaliana reaguju na prisustvo ekstrakta vlašca (koncentracije 100 mg/L) i kvercetina (koncentracije 15.11 mg/L) u hranljivoj podlozi. Dužina hipokotila je iz merena petog, sedmog i desetog dana nakon klijanja semena. Na elongaciju hipokotila značajno utiče prisustvo kvercetina u podlozi. Zahvaljući DAB (3,3´- diaminobenzidin) bojenju detekto vana je akumulacija H2O2 u biljnom tkivu klijanaca A. thaliana starosti 10 dana. Ovaj rezultat je u saglasnosti sa povećanom aktivnošću antioksidativnog enzima peroksidaza kod klijanaca koji su rasli na podlozi sa ekstraktom vlašca. Takođe je određena i aktivnost enzima glutation transferaza, koji se dominantno nalazi u citosolu i učestvuje u procesima detoksikacije biljne ćelije. Opisane promene u aktivnosti antioksidativnih enzima klijanaca model biljke na podlozi sa ekstraktom vlašca, usmeravaju nas ka novim koracima u istraživanju sa mogućnošću razvoja potencijalnih bioherbicida
AB  - Chive leaf extract is a rich source of flavonols (kaempferol, quercetin and izorahmentin) and flavonoid glycosides (kaempferol-3-glucoside and quercetin-3-glucoside). These compounds are a very important group of flavonoids that have a broad range of biological activities. The aim of this study is to investigate the response of Arabidopsis thaliana to the presence of chive extract (concentration of 100 mg/L) and quercetin (concentration of 15.11 mg/L) in the growth medium. The length of hypocotyl was measured on the 5th, 7th, and 10th day after the germination of seeds. The presence of quercetin in the growth medium significantly effects the elongation of the hypocotyl. Accumulation of H2O2 in the plant tissue of seedlings was detected by DAB staining (3,3’-diami nobenzidine). These results correspond with increased activity of antioxidative enzyme peroxidase in seedlings that grow on the medium with chive extract. The activity of glutathione transferase was also determined. This enzyme is predominantly located in the cytosol and participates in the process of detoxification of plant cells. The described changes in antioxidative enzyme activities in plant model seedlings on growth medium with chive extract guide us towards new steps in research with the possible development of a potential bioherbicide
PB  - Novi Sad: Naučno-istraživačko društvo studenata biologije i ekologije „Josif Pančić“
C3  - Knjiga izvoda: 4. Konferencija studenata biologije, ekologije i zaštite životne sredine: Ekobiomorfa; 2022 Nov 25-27; Novi Sad, Serbia
T1  - Ispitivanje uticaja ekstrakta vlašca (Allium schoenoprasum L.) i kvercetina na morfološke i fiziološke karakteristike klijanaca Arabidopsis thaliana
T1  - Assessment of the effect of chive extract (Allium schoenoprasum L.) and quercetin on morphological and physological characteristics of Arabidopsis thaliana seedlings
SP  - 12
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6493
ER  - 

@conference{
author = "Ninković, Nina and Tubić, Ljiljana",
year = "2022",
abstract = "Ekstrakt listova vlašca je bogat flavonolima (kemferol, kvercetin i izoramnetin) i flavonol nim glikozidima (kemferol-3-O-glikozid i kvercetin-3-O-glikozid). Ova jedinjenja predstavljaju važnu grupu flanovoida sa širokim spektrom biološke aktivnosti. Cilj ovog istraživanja je da se ispita kako klijanci model biljke A. thaliana reaguju na prisustvo ekstrakta vlašca (koncentracije 100 mg/L) i kvercetina (koncentracije 15.11 mg/L) u hranljivoj podlozi. Dužina hipokotila je iz merena petog, sedmog i desetog dana nakon klijanja semena. Na elongaciju hipokotila značajno utiče prisustvo kvercetina u podlozi. Zahvaljući DAB (3,3´- diaminobenzidin) bojenju detekto vana je akumulacija H2O2 u biljnom tkivu klijanaca A. thaliana starosti 10 dana. Ovaj rezultat je u saglasnosti sa povećanom aktivnošću antioksidativnog enzima peroksidaza kod klijanaca koji su rasli na podlozi sa ekstraktom vlašca. Takođe je određena i aktivnost enzima glutation transferaza, koji se dominantno nalazi u citosolu i učestvuje u procesima detoksikacije biljne ćelije. Opisane promene u aktivnosti antioksidativnih enzima klijanaca model biljke na podlozi sa ekstraktom vlašca, usmeravaju nas ka novim koracima u istraživanju sa mogućnošću razvoja potencijalnih bioherbicida, Chive leaf extract is a rich source of flavonols (kaempferol, quercetin and izorahmentin) and flavonoid glycosides (kaempferol-3-glucoside and quercetin-3-glucoside). These compounds are a very important group of flavonoids that have a broad range of biological activities. The aim of this study is to investigate the response of Arabidopsis thaliana to the presence of chive extract (concentration of 100 mg/L) and quercetin (concentration of 15.11 mg/L) in the growth medium. The length of hypocotyl was measured on the 5th, 7th, and 10th day after the germination of seeds. The presence of quercetin in the growth medium significantly effects the elongation of the hypocotyl. Accumulation of H2O2 in the plant tissue of seedlings was detected by DAB staining (3,3’-diami nobenzidine). These results correspond with increased activity of antioxidative enzyme peroxidase in seedlings that grow on the medium with chive extract. The activity of glutathione transferase was also determined. This enzyme is predominantly located in the cytosol and participates in the process of detoxification of plant cells. The described changes in antioxidative enzyme activities in plant model seedlings on growth medium with chive extract guide us towards new steps in research with the possible development of a potential bioherbicide",
publisher = "Novi Sad: Naučno-istraživačko društvo studenata biologije i ekologije „Josif Pančić“",
journal = "Knjiga izvoda: 4. Konferencija studenata biologije, ekologije i zaštite životne sredine: Ekobiomorfa; 2022 Nov 25-27; Novi Sad, Serbia",
title = "Ispitivanje uticaja ekstrakta vlašca (Allium schoenoprasum L.) i kvercetina na morfološke i fiziološke karakteristike klijanaca Arabidopsis thaliana, Assessment of the effect of chive extract (Allium schoenoprasum L.) and quercetin on morphological and physological characteristics of Arabidopsis thaliana seedlings",
pages = "12-13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6493"
}

Ninković, N.,& Tubić, L.. (2022). Ispitivanje uticaja ekstrakta vlašca (Allium schoenoprasum L.) i kvercetina na morfološke i fiziološke karakteristike klijanaca Arabidopsis thaliana. in Knjiga izvoda: 4. Konferencija studenata biologije, ekologije i zaštite životne sredine: Ekobiomorfa; 2022 Nov 25-27; Novi Sad, Serbia
Novi Sad: Naučno-istraživačko društvo studenata biologije i ekologije „Josif Pančić“., 12-13.
https://hdl.handle.net/21.15107/rcub_ibiss_6493

Ninković N, Tubić L. Ispitivanje uticaja ekstrakta vlašca (Allium schoenoprasum L.) i kvercetina na morfološke i fiziološke karakteristike klijanaca Arabidopsis thaliana. in Knjiga izvoda: 4. Konferencija studenata biologije, ekologije i zaštite životne sredine: Ekobiomorfa; 2022 Nov 25-27; Novi Sad, Serbia. 2022;:12-13.
https://hdl.handle.net/21.15107/rcub_ibiss_6493 .

Ninković, Nina, Tubić, Ljiljana, "Ispitivanje uticaja ekstrakta vlašca (Allium schoenoprasum L.) i kvercetina na morfološke i fiziološke karakteristike klijanaca Arabidopsis thaliana" in Knjiga izvoda: 4. Konferencija studenata biologije, ekologije i zaštite životne sredine: Ekobiomorfa; 2022 Nov 25-27; Novi Sad, Serbia (2022):12-13,
https://hdl.handle.net/21.15107/rcub_ibiss_6493 .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Gašić, Uroš
AU  - Šajkunić, Sanja
AU  - Cingel, Aleksandar
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/407
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4789
AB  - French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.
PB  - Basel: MDPI
T2  - Plants
T1  - UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle
IS  - 3
VL  - 11
DO  - 10.3390/plants11030407
SP  - 407
ER  - 

@article{
author = "Devrnja, Nina and Gašić, Uroš and Šajkunić, Sanja and Cingel, Aleksandar and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena",
year = "2022",
abstract = "French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antiox-idant activity statistically comparable with positive control Trolox, was chosen for testing of anti-feedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle",
number = "3",
volume = "11",
doi = "10.3390/plants11030407",
pages = "407"
}

Devrnja, N., Gašić, U., Šajkunić, S., Cingel, A., Stupar, S., Tubić, L.,& Savić, J.. (2022). UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants
Basel: MDPI., 11(3), 407.
https://doi.org/10.3390/plants11030407

Devrnja N, Gašić U, Šajkunić S, Cingel A, Stupar S, Tubić L, Savić J. UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle. in Plants. 2022;11(3):407.
doi:10.3390/plants11030407 .

Devrnja, Nina, Gašić, Uroš, Šajkunić, Sanja, Cingel, Aleksandar, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, "UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle" in Plants, 11, no. 3 (2022):407,
https://doi.org/10.3390/plants11030407 . .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5050
AB  - Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - French marigold essential oil affected DNA methylation in potato
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5050
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses
to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the
level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown
promising prospects as novel priming inducers. Still, little is known about their potential to induce
epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula
L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to
EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal.
Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM)
DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR,
and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and
treated samples were analyzed. The average methylation level of the whole genome was 72.29%
and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly
occurred in the CG context compared to CHH and CHG. In general, hypomethylated
sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially
methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein
processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes
associated with these pathways, as well as different defense responses in potato (GO:0006952),
will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress
conditions.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "French marigold essential oil affected DNA methylation in potato",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5050"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5050 .

TY  - CONF
AU  - Šajkunić, Sanja
AU  - Dragićević, Milan
AU  - Milutinović, Milica
AU  - Stupar, Sofija
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Devrnja, Nina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5051
AB  - Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
T1  - French marigold essential oil affected DNA methylation in potato
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5051
ER  - 

@conference{
author = "Šajkunić, Sanja and Dragićević, Milan and Milutinović, Milica and Stupar, Sofija and Tubić, Ljiljana and Savić, Jelena and Devrnja, Nina",
year = "2022",
abstract = "Different stressors from the environment can prime defense and enable enhanced responses to subsequent stress exposure. It has been proposed that induced epigenetic changes, on the level of DNA and histones, could be involved in this response. Plant essential oils (EOs) have shown promising prospects as novel priming inducers. Still, little is known about their potential to induce epigenetic alternations. In this study, we examined the effect of the French marigold (Tagetes patula L.) EO on DNA methylation of potato (Solanum tuberosum L.). Potato plants were exposed to EO for 3 consecutive days for 8h each day, and samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing (WGBS) was used to identify differentially methylated (DM) DNA sequences among control and EO-exposed samples. Promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic regions, each at a differential methylation ratio 0.1 to 0.25 between control and treated samples were analyzed. The average methylation level of the whole genome was 72.29% and 69.23% in control and exposed samples, respectively. Differential DNA methylation has predominantly occurred in the CG context compared to CHH and CHG. In general, hypomethylated sequences outnumbered hypermethylated ones. Functional annotation clustering of differentially methylated genes distinguished 6 affected KEGG pathways – endocytosis (sot04144) and protein processing in the endoplasmatic reticulum (sot04141) among others. The expression of DM genes associated with these pathways, as well as different defense responses in potato (GO:0006952), will be analyzed subsequently in potato plants challenged with different abiotic and biotic stress conditions.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.",
title = "French marigold essential oil affected DNA methylation in potato",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5051"
}

Šajkunić, S., Dragićević, M., Milutinović, M., Stupar, S., Tubić, L., Savić, J.,& Devrnja, N.. (2022). French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5051

Šajkunić S, Dragićević M, Milutinović M, Stupar S, Tubić L, Savić J, Devrnja N. French marigold essential oil affected DNA methylation in potato. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia.. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

Šajkunić, Sanja, Dragićević, Milan, Milutinović, Milica, Stupar, Sofija, Tubić, Ljiljana, Savić, Jelena, Devrnja, Nina, "French marigold essential oil affected DNA methylation in potato" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5051 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5053
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - P6
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5053
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "P6",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5053"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:P6.
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):P6,
https://hdl.handle.net/21.15107/rcub_ibiss_5053 .

TY  - CONF
AU  - Savić, Jelena
AU  - Stupar, Sofija
AU  - Devrnja, Nina
AU  - Šajkunić, Sanja
AU  - Tubić, Ljiljana
PY  - 2022
UR  - https://www.plantslo.org/wp/wp-content/uploads/2022/09/PlantsInChangingEnvironment22AbstractBook.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5052
AB  - Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.
PB  - Ljubljana: Slovenian Society of Plant Biology
C3  - Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
T1  - Role of glutathione in potato response to French marigold essential oil
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5052
ER  - 

@conference{
author = "Savić, Jelena and Stupar, Sofija and Devrnja, Nina and Šajkunić, Sanja and Tubić, Ljiljana",
year = "2022",
abstract = "Volatile organic compounds (VOCs) have a role in communication of plants with other organisms. The effect of VOCs from French marigold (Tagetes patula L.) essential oil (EO) on potato (Solanum tuberosum L.) plants was studied to examine the interaction between this ornamental plant species and potato as an important agricultural crop. According to comprehensive microarray analysis, EO affected the expression of numerous potato genes after 8 h of exposure. From 124 identified transcripts annotated to glutathione metabolism pathway in potato (sot00480), 43 were with significantly altered expression (35%), and 42 of them were up-regulated. Glutathione (GSH) is an essential low-molecular metabolite, which participates in the removal of reactive oxygen species (ROS), stress-generated signal molecules involved in immune responses. Transitions between glutathione reduced (GSH) and oxidized forms (GSSG) are finely controlled by complex enzymatic machinery. Thus, in order to examine in more details the role of glutathione in potato ROS balance after perception of VOCs, the expression of 10 selected genes involved in glutathione metabolism was further analysed by the qRT-PCR method. For this purpose potato plants were exposed to EO for 8 h during one or three consecutive days. Samples were collected immediately after EO removal, or after 5 and 10 days. Results showed that gene coding for γ-glutamylcysteine synthetase (γ-GCS), first in the line of GSH synthesis, had the highest level of expression after the first 8 h of exposure, indicating a fast response to VOCs perception. In other experimental groups synthesis of GSH, in which were involved GSH synthase (GSS) and glutamyltranspeptidase (GGT), was decreased. A similar pattern was observed for genes coding for enzymes involved in redox glutathione transduction (glutathione reductase - GR, isocitrate dehydrogenase - IDH, and dehydroascorbate reductase-like protein - DHAR). However, the highest alternation in expression between exposed potato and corresponding controls was observed for glutathione S-transferase (GST) genes, indicating that the glutathione-based response mechanism is an important component of ROS scavenging mechanisms in VOCs-induced responses of the potato. In addition, the potential of glutathione to sequester and detoxify exogenous VOCs by glutathione S-transferases, opens new research avenues.",
publisher = "Ljubljana: Slovenian Society of Plant Biology",
journal = "Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.",
title = "Role of glutathione in potato response to French marigold essential oil",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5052"
}

Savić, J., Stupar, S., Devrnja, N., Šajkunić, S.,& Tubić, L.. (2022). Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.
Ljubljana: Slovenian Society of Plant Biology., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052

Savić J, Stupar S, Devrnja N, Šajkunić S, Tubić L. Role of glutathione in potato response to French marigold essential oil. in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia.. 2022;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

Savić, Jelena, Stupar, Sofija, Devrnja, Nina, Šajkunić, Sanja, Tubić, Ljiljana, "Role of glutathione in potato response to French marigold essential oil" in Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. (2022):17,
https://hdl.handle.net/21.15107/rcub_ibiss_5052 .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Kaleri, Abdul Rasheed
AU  - Ninković, Slavica
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4303
AB  - De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168554
SP  - 8554
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Kaleri, Abdul Rasheed and Ninković, Slavica and Tubić, Ljiljana and Cingel, Aleksandar and Ćosić, Tatjana",
year = "2021",
abstract = "De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs",
number = "16",
volume = "22",
doi = "10.3390/ijms22168554",
pages = "8554"
}

Raspor, M., Motyka, V., Kaleri, A. R., Ninković, S., Tubić, L., Cingel, A.,& Ćosić, T.. (2021). Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8554.
https://doi.org/10.3390/ijms22168554

Raspor M, Motyka V, Kaleri AR, Ninković S, Tubić L, Cingel A, Ćosić T. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences. 2021;22(16):8554.
doi:10.3390/ijms22168554 .

Raspor, Martin, Motyka, Václav, Kaleri, Abdul Rasheed, Ninković, Slavica, Tubić, Ljiljana, Cingel, Aleksandar, Ćosić, Tatjana, "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs" in International Journal of Molecular Sciences, 22, no. 16 (2021):8554,
https://doi.org/10.3390/ijms22168554 . .

TY  - JOUR
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/8/1599
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8401273
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4456
AB  - A plant's main mechanism to diminish the effects caused by high free radical levels generated during high irradiance is the synthesis of various secondary metabolites. In addition to interspecies differences, their concentrations may be influenced by genetic, ontogenic, morphogenetic or environmental factors. We investigated the influence of genetic (genotypes from different natural habitats) and environmental (contrasting light regimes as well as successive parts of the vegetation period) variability on the accumulation of 10 selected phenolic compounds (phenolic acids, flavonoids, and xanthones) in Iris variegata genotypes. Genotypes originated from either sun-exposed or shaded natural habitats were transplanted to two experimental light treatments (high light intensity with a higher R/FR ratio and low light intensity with a lower R/FR ratio). Significant impacts of both genetic and environmental seasonal variability (spring, summer and fall during the vegetation period) on phenolic compound profiles were detected. Their highest amounts were detected in spring. The magnitude of difference between light treatments (high vs. low light intensity) and the direction of this change varied depending on the secondary compound class. Phenotypic correlations among the 10 analyzed secondary metabolites differed across the experimental light treatments and their number decreased from spring to fall.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.
IS  - 8
VL  - 10
DO  - 10.3390/plants10081599
SP  - 1599
ER  - 

@article{
author = "Živković, Uroš and Avramov, Stevan and Miljković, Danijela and Barišić Klisarić, Nataša and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2021",
abstract = "A plant's main mechanism to diminish the effects caused by high free radical levels generated during high irradiance is the synthesis of various secondary metabolites. In addition to interspecies differences, their concentrations may be influenced by genetic, ontogenic, morphogenetic or environmental factors. We investigated the influence of genetic (genotypes from different natural habitats) and environmental (contrasting light regimes as well as successive parts of the vegetation period) variability on the accumulation of 10 selected phenolic compounds (phenolic acids, flavonoids, and xanthones) in Iris variegata genotypes. Genotypes originated from either sun-exposed or shaded natural habitats were transplanted to two experimental light treatments (high light intensity with a higher R/FR ratio and low light intensity with a lower R/FR ratio). Significant impacts of both genetic and environmental seasonal variability (spring, summer and fall during the vegetation period) on phenolic compound profiles were detected. Their highest amounts were detected in spring. The magnitude of difference between light treatments (high vs. low light intensity) and the direction of this change varied depending on the secondary compound class. Phenotypic correlations among the 10 analyzed secondary metabolites differed across the experimental light treatments and their number decreased from spring to fall.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.",
number = "8",
volume = "10",
doi = "10.3390/plants10081599",
pages = "1599"
}

Živković, U., Avramov, S., Miljković, D., Barišić Klisarić, N., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2021). Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.. in Plants (Basel, Switzerland)
Basel: MDPI., 10(8), 1599.
https://doi.org/10.3390/plants10081599

Živković U, Avramov S, Miljković D, Barišić Klisarić N, Tubić L, Mišić D, Šiler B, Tarasjev A. Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.. in Plants (Basel, Switzerland). 2021;10(8):1599.
doi:10.3390/plants10081599 .

Živković, Uroš, Avramov, Stevan, Miljković, Danijela, Barišić Klisarić, Nataša, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L." in Plants (Basel, Switzerland), 10, no. 8 (2021):1599,
https://doi.org/10.3390/plants10081599 . .

TY  - CONF
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4115
AB  - Higher plants have developed protection mechanisms in order to diminish the
effects caused by the presence of free radicals generated during high
irradiance, and one of the detoxification mechanisms is the synthesis of
secondary metabolites. In this study we investigated the amount and pattern
of individual phenotypic responses (represented through targeted phenolics
content in 68 genotypes of Iris variegata) to seasonal changes in
environmental conditions under two experimental light regimes. The goal was
to examine how environmental stressors mold the interrelationships between
metabolite traits. Genotypes of I. variegata were collected in Deliblato sands
Special Nature Reserve in Serbia, from two types of natural habitats (exposed
and shade) and transferred into the experimental field conditions providing
either 1. high light intensity and higher red / far red light ratio and 2. low light
intensity and lower red / far red light ratio. After period of acclimatization
leaves were collected during spring, summer and fall of one experimental year
and subjected to UHPLC/qqqMS quantification of 10 selected phenolic
compounds belonging to the groups of phenolic acids, flavonoids and
xanthones. The correlations between all studied compounds within two light
treatments and three seasons were in almost all cases significant and positive.
Number of statistically significant correlations markedly decreased through
the vegetative period in both light treatments. The experiment was conducted
on a large number of genotypes of I. variegata and points to the need of
involving a number of factors in future ecological and evolutionary researches.
PB  - Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka
C3  - IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020
T1  - Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions
SP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4115
ER  - 

@conference{
author = "Živković, Uroš and Avramov, Stevan and Barišić Klisarić, Nataša and Miljković, Danijela and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2020",
abstract = "Higher plants have developed protection mechanisms in order to diminish the
effects caused by the presence of free radicals generated during high
irradiance, and one of the detoxification mechanisms is the synthesis of
secondary metabolites. In this study we investigated the amount and pattern
of individual phenotypic responses (represented through targeted phenolics
content in 68 genotypes of Iris variegata) to seasonal changes in
environmental conditions under two experimental light regimes. The goal was
to examine how environmental stressors mold the interrelationships between
metabolite traits. Genotypes of I. variegata were collected in Deliblato sands
Special Nature Reserve in Serbia, from two types of natural habitats (exposed
and shade) and transferred into the experimental field conditions providing
either 1. high light intensity and higher red / far red light ratio and 2. low light
intensity and lower red / far red light ratio. After period of acclimatization
leaves were collected during spring, summer and fall of one experimental year
and subjected to UHPLC/qqqMS quantification of 10 selected phenolic
compounds belonging to the groups of phenolic acids, flavonoids and
xanthones. The correlations between all studied compounds within two light
treatments and three seasons were in almost all cases significant and positive.
Number of statistically significant correlations markedly decreased through
the vegetative period in both light treatments. The experiment was conducted
on a large number of genotypes of I. variegata and points to the need of
involving a number of factors in future ecological and evolutionary researches.",
publisher = "Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka",
journal = "IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020",
title = "Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions",
pages = "127",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4115"
}

Živković, U., Avramov, S., Barišić Klisarić, N., Miljković, D., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2020). Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions. in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020
Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka., 127.
https://hdl.handle.net/21.15107/rcub_ibiss_4115

Živković U, Avramov S, Barišić Klisarić N, Miljković D, Tubić L, Mišić D, Šiler B, Tarasjev A. Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions. in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020. 2020;:127.
https://hdl.handle.net/21.15107/rcub_ibiss_4115 .

Živković, Uroš, Avramov, Stevan, Barišić Klisarić, Nataša, Miljković, Danijela, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions" in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020 (2020):127,
https://hdl.handle.net/21.15107/rcub_ibiss_4115 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Avramović, Miloš
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5593
AB  - Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5593
ER  - 

@conference{
author = "Stupar, Sofija and Avramović, Miloš and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2019",
abstract = "Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5593"
}

Stupar, S., Avramović, M., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2019). Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593

Stupar S, Avramović M, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

Stupar, Sofija, Avramović, Miloš, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Savić, Jelena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0304423818308938?via%3Dihub
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0304423818308938
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3224
AB  - Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.
T2  - Scientia Horticulturae
T1  - Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus
VL  - 247
DO  - 10.1016/j.scienta.2018.12.021
SP  - 362
EP  - 372
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Devrnja, Nina and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Savić, Jelena",
year = "2019",
abstract = "Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.",
journal = "Scientia Horticulturae",
title = "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus",
volume = "247",
doi = "10.1016/j.scienta.2018.12.021",
pages = "362-372"
}

Zdravković-Korać, S., Tubić, L., Devrnja, N., Ćalić, D., Milojević, J., Belić, M.,& Savić, J.. (2019). Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae, 247, 362-372.
https://doi.org/10.1016/j.scienta.2018.12.021

Zdravković-Korać S, Tubić L, Devrnja N, Ćalić D, Milojević J, Belić M, Savić J. Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae. 2019;247:362-372.
doi:10.1016/j.scienta.2018.12.021 .

Zdravković-Korać, Snežana, Tubić, Ljiljana, Devrnja, Nina, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Savić, Jelena, "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus" in Scientia Horticulturae, 247 (2019):362-372,
https://doi.org/10.1016/j.scienta.2018.12.021 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Ghalawenji, Nabil
AU  - Zdravković-Korać, Snežana
PY  - 2019
UR  - http://link.springer.com/10.1007/s11240-019-01595-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3310
AB  - Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)
DO  - 10.1007/s11240-019-01595-6
ER  - 

@article{
author = "Banjac, Nevena and Vinterhalter, Branka and Krstić Milošević, Dijana and Milojević, Jelena and Tubić, Ljiljana and Ghalawenji, Nabil and Zdravković-Korać, Snežana",
year = "2019",
abstract = "Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)",
doi = "10.1007/s11240-019-01595-6"
}

Banjac, N., Vinterhalter, B., Krstić Milošević, D., Milojević, J., Tubić, L., Ghalawenji, N.,& Zdravković-Korać, S.. (2019). Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC).
https://doi.org/10.1007/s11240-019-01595-6

Banjac N, Vinterhalter B, Krstić Milošević D, Milojević J, Tubić L, Ghalawenji N, Zdravković-Korać S. Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). in Plant Cell, Tissue and Organ Culture (PCTOC). 2019;.
doi:10.1007/s11240-019-01595-6 .

Banjac, Nevena, Vinterhalter, Branka, Krstić Milošević, Dijana, Milojević, Jelena, Tubić, Ljiljana, Ghalawenji, Nabil, Zdravković-Korać, Snežana, "Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)" in Plant Cell, Tissue and Organ Culture (PCTOC) (2019),
https://doi.org/10.1007/s11240-019-01595-6 . .

TY  - CONF
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6494
AB  - During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions. Light is one of the most dynamic components of the terrestrial environment
that affects plant physiology and development. Optimization of light harvesting for photoautotrophic growth inevitably induces specific adjustments in all aspects of plant phenotype: morphology, anatomy, phytochemical composition, flowering phenology etc. As sessile organisms
capable of perceiving quantitative and qualitative features of light surroundings, plants need to
be particularly plastic in their response to different light environments. For this study genotypes of Iris variegata that occupy different light habitats in Deliblato sands were selected: a) open habitats
where they were exposed to full sunlight and b) woodland understories with lower light intensity
and changed light quality (vegetative shade). Specimens of those I. variegata genotypes were
grown under two experimental light treatments: 1.) high intensity and higher red-far red light ratio
and 2.) low intensity and lower red-far red light ratio. Leaves were collected during spring, summer
and autumn of one experimental year, dried in silica gel and extracted with methanol. Samples
were subjected to UHPLC/qqqMS profiling of phenolics (phenolic acids, flavonoids, and xanthones), and subsequently to chemometric analysis. Results showed that dissimilar light conditions
in applied treatments markedly affected I. variegata phenolics composition. Repeated measures
model ANOVA revealed a significant effect of seasons for all analyzed phenolics. Concentrations of
half of the analyzed chemical compounds were significantly different under two light treatments.
The correlations between traits were in almost all cases significant and positive.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions
SP  - 97
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6494
ER  - 

@conference{
author = "Živković, Uroš and Avramov, Stevan and Barišić Klisarić, Nataša and Miljković, Danijela and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2018",
abstract = "During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions. Light is one of the most dynamic components of the terrestrial environment
that affects plant physiology and development. Optimization of light harvesting for photoautotrophic growth inevitably induces specific adjustments in all aspects of plant phenotype: morphology, anatomy, phytochemical composition, flowering phenology etc. As sessile organisms
capable of perceiving quantitative and qualitative features of light surroundings, plants need to
be particularly plastic in their response to different light environments. For this study genotypes of Iris variegata that occupy different light habitats in Deliblato sands were selected: a) open habitats
where they were exposed to full sunlight and b) woodland understories with lower light intensity
and changed light quality (vegetative shade). Specimens of those I. variegata genotypes were
grown under two experimental light treatments: 1.) high intensity and higher red-far red light ratio
and 2.) low intensity and lower red-far red light ratio. Leaves were collected during spring, summer
and autumn of one experimental year, dried in silica gel and extracted with methanol. Samples
were subjected to UHPLC/qqqMS profiling of phenolics (phenolic acids, flavonoids, and xanthones), and subsequently to chemometric analysis. Results showed that dissimilar light conditions
in applied treatments markedly affected I. variegata phenolics composition. Repeated measures
model ANOVA revealed a significant effect of seasons for all analyzed phenolics. Concentrations of
half of the analyzed chemical compounds were significantly different under two light treatments.
The correlations between traits were in almost all cases significant and positive.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions",
pages = "97-98",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6494"
}

Živković, U., Avramov, S., Barišić Klisarić, N., Miljković, D., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2018). Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 97-98.
https://hdl.handle.net/21.15107/rcub_ibiss_6494

Živković U, Avramov S, Barišić Klisarić N, Miljković D, Tubić L, Mišić D, Šiler B, Tarasjev A. Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:97-98.
https://hdl.handle.net/21.15107/rcub_ibiss_6494 .

Živković, Uroš, Avramov, Stevan, Barišić Klisarić, Nataša, Miljković, Danijela, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):97-98,
https://hdl.handle.net/21.15107/rcub_ibiss_6494 .

TY  - CONF
AU  - Tubić, Ljiljana
AU  - Simin, Nataša
AU  - Živković, Sanja
AU  - Bekvalac, Kristina
AU  - Savić, Jelena
AU  - Momčilović, Miloš
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5453
AB  - Chive (Allium schoenoprasum L.) is a small bulbous perennial which is used as a spice plant and
as a culinary herb. This plant contains compounds with potent antioxidant activity and has beneficial
effect on human health. Chive can be easily grown without special care. Accordingly, it would
be the best that people grow spice and healthy plants on their own balconies and terraces to have
fresh raw plant like chive every day. The objective of this study was to determine the impact of urban
environmental factors on the quality of chives planted at 5 different locations in Belgrade. The parameters of quality were phenolics profile and heavy metals content, while exposure to stress
was determined by measuring the activity of antioxidant enzymes, peroxidases (POX) and superoxide
dismutases (SOD) in fresh plants. The phenolics profile of plant aerial parts was characterized
by LC-MS/MS. Twelve phenolic compounds were quantified, among which kaempferol 3-O-glucoside
and quercetin 3-O-glucoside were dominant. Positive correlation between POX/SOD activity
and concentrations of the forementioned compounds was observed. The concentrations of Cd,
Cr, Cu, Pb and Zn in the leaves of investigated chives were measured using ICP-OES technique.
The levels of Cd in edible parts of chive at all investigated locations were higher than permissible
limit. Mean Cr concentration in leaves of chive was high only in central urban zone. The process of
urbanization has been expanded with the negative consequences on plants, which can be seen
from increased POX activity and high levels of heavy metals in leaves of chives.
PB  - Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Fresh chives in pots on the Belgrade’s balconies and terraces How do the urban environmental factors affect their quality?
SP  - 53
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5453
ER  - 

@conference{
author = "Tubić, Ljiljana and Simin, Nataša and Živković, Sanja and Bekvalac, Kristina and Savić, Jelena and Momčilović, Miloš",
year = "2018",
abstract = "Chive (Allium schoenoprasum L.) is a small bulbous perennial which is used as a spice plant and
as a culinary herb. This plant contains compounds with potent antioxidant activity and has beneficial
effect on human health. Chive can be easily grown without special care. Accordingly, it would
be the best that people grow spice and healthy plants on their own balconies and terraces to have
fresh raw plant like chive every day. The objective of this study was to determine the impact of urban
environmental factors on the quality of chives planted at 5 different locations in Belgrade. The parameters of quality were phenolics profile and heavy metals content, while exposure to stress
was determined by measuring the activity of antioxidant enzymes, peroxidases (POX) and superoxide
dismutases (SOD) in fresh plants. The phenolics profile of plant aerial parts was characterized
by LC-MS/MS. Twelve phenolic compounds were quantified, among which kaempferol 3-O-glucoside
and quercetin 3-O-glucoside were dominant. Positive correlation between POX/SOD activity
and concentrations of the forementioned compounds was observed. The concentrations of Cd,
Cr, Cu, Pb and Zn in the leaves of investigated chives were measured using ICP-OES technique.
The levels of Cd in edible parts of chive at all investigated locations were higher than permissible
limit. Mean Cr concentration in leaves of chive was high only in central urban zone. The process of
urbanization has been expanded with the negative consequences on plants, which can be seen
from increased POX activity and high levels of heavy metals in leaves of chives.",
publisher = "Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Fresh chives in pots on the Belgrade’s balconies and terraces How do the urban environmental factors affect their quality?",
pages = "53-54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5453"
}

Tubić, L., Simin, N., Živković, S., Bekvalac, K., Savić, J.,& Momčilović, M.. (2018). Fresh chives in pots on the Belgrade’s balconies and terraces How do the urban environmental factors affect their quality?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 53-54.
https://hdl.handle.net/21.15107/rcub_ibiss_5453

Tubić L, Simin N, Živković S, Bekvalac K, Savić J, Momčilović M. Fresh chives in pots on the Belgrade’s balconies and terraces How do the urban environmental factors affect their quality?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:53-54.
https://hdl.handle.net/21.15107/rcub_ibiss_5453 .

Tubić, Ljiljana, Simin, Nataša, Živković, Sanja, Bekvalac, Kristina, Savić, Jelena, Momčilović, Miloš, "Fresh chives in pots on the Belgrade’s balconies and terraces How do the urban environmental factors affect their quality?" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):53-54,
https://hdl.handle.net/21.15107/rcub_ibiss_5453 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Tešević, Vele
AU  - Stanković, Jovana
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4329
AB  - When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4329
ER  - 

@conference{
author = "Stupar, Sofija and Tešević, Vele and Stanković, Jovana and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2018",
abstract = "When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil",
pages = "58",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4329"
}

Stupar, S., Tešević, V., Stanković, J., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2018). Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329

Stupar S, Tešević V, Stanković J, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:58.
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .

Stupar, Sofija, Tešević, Vele, Stanković, Jovana, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):58,
https://hdl.handle.net/21.15107/rcub_ibiss_4329 .

TY  - JOUR
AU  - Belić, Maja
AU  - Savić, Jelena
AU  - Tubić, Ljiljana
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2017
UR  - http://link.springer.com/10.1007/s11240-017-1193-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2608
AB  - High variability in regeneration capacity has previously been observed within a population of seedlings in several spinach cultivars. The cultivar “Matador” exhibits particularly low regeneration potential, and the majority of lines obtained in our previous study displayed a stable embryogenic capacity only for a limited period of time. In order to shorten the time required for embryogenic capacity assessment for individual lines, a model system for the rapid evaluation of embryogenic capacity was developed. This model system was based on the expression of a gene encoding spinach ribosome-inactivating protein (SoRIP2), which showed low expression levels in roots grown under non-inductive conditions. Induction of globular somatic embryos (SEs) resulted in a 285-fold increase in SoRIP2 expression that dropped to the control level beyond cotyledonary-stage SEs. The model system was tested by comparing the expression of SoRIP2 and the index of embryo-forming capacity (EFC), which integrates the frequency of regeneration and the mean SE number per root explant. Comparisons were always made within the same line, and the expression of SoRIP2 and the EFC index were determined 4 and 12 weeks after starting induction treatment, respectively. High positive correlations between SoRIP2 expression and EFC were obtained for the two factors that influenced embryogenic capacity the most: genotype (r2 = 0.81) and photoperiod (r2 = 0.92). The results indicate that the expression of SoRIP2 can be successfully used for early evaluation of regeneration capacity of individual lines, before SEs can be seen with the aid of a stereomicroscope, even 8 weeks earlier than by the conventional method.
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Expression of the gene for ribosome-inactivating protein, SoRIP2, as a tool for the evaluation of somatic embryogenesis in spinach
DO  - 10.1007/s11240-017-1193-8
ER  - 

@article{
author = "Belić, Maja and Savić, Jelena and Tubić, Ljiljana and Devrnja, Nina and Ćalić, Dušica and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2017",
abstract = "High variability in regeneration capacity has previously been observed within a population of seedlings in several spinach cultivars. The cultivar “Matador” exhibits particularly low regeneration potential, and the majority of lines obtained in our previous study displayed a stable embryogenic capacity only for a limited period of time. In order to shorten the time required for embryogenic capacity assessment for individual lines, a model system for the rapid evaluation of embryogenic capacity was developed. This model system was based on the expression of a gene encoding spinach ribosome-inactivating protein (SoRIP2), which showed low expression levels in roots grown under non-inductive conditions. Induction of globular somatic embryos (SEs) resulted in a 285-fold increase in SoRIP2 expression that dropped to the control level beyond cotyledonary-stage SEs. The model system was tested by comparing the expression of SoRIP2 and the index of embryo-forming capacity (EFC), which integrates the frequency of regeneration and the mean SE number per root explant. Comparisons were always made within the same line, and the expression of SoRIP2 and the EFC index were determined 4 and 12 weeks after starting induction treatment, respectively. High positive correlations between SoRIP2 expression and EFC were obtained for the two factors that influenced embryogenic capacity the most: genotype (r2 = 0.81) and photoperiod (r2 = 0.92). The results indicate that the expression of SoRIP2 can be successfully used for early evaluation of regeneration capacity of individual lines, before SEs can be seen with the aid of a stereomicroscope, even 8 weeks earlier than by the conventional method.",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Expression of the gene for ribosome-inactivating protein, SoRIP2, as a tool for the evaluation of somatic embryogenesis in spinach",
doi = "10.1007/s11240-017-1193-8"
}

Belić, M., Savić, J., Tubić, L., Devrnja, N., Ćalić, D., Zdravković-Korać, S.,& Milojević, J.. (2017). Expression of the gene for ribosome-inactivating protein, SoRIP2, as a tool for the evaluation of somatic embryogenesis in spinach. in Plant Cell, Tissue and Organ Culture (PCTOC).
https://doi.org/10.1007/s11240-017-1193-8

Belić M, Savić J, Tubić L, Devrnja N, Ćalić D, Zdravković-Korać S, Milojević J. Expression of the gene for ribosome-inactivating protein, SoRIP2, as a tool for the evaluation of somatic embryogenesis in spinach. in Plant Cell, Tissue and Organ Culture (PCTOC). 2017;.
doi:10.1007/s11240-017-1193-8 .

Belić, Maja, Savić, Jelena, Tubić, Ljiljana, Devrnja, Nina, Ćalić, Dušica, Zdravković-Korać, Snežana, Milojević, Jelena, "Expression of the gene for ribosome-inactivating protein, SoRIP2, as a tool for the evaluation of somatic embryogenesis in spinach" in Plant Cell, Tissue and Organ Culture (PCTOC) (2017),
https://doi.org/10.1007/s11240-017-1193-8 . .

TY  - THES
AU  - Tubić, Ljiljana
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3374
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:11875/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025090226
UR  - http://nardus.mpn.gov.rs/123456789/5953
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2427
AB  - Šalot (Allium ascalonicum L.) i vlašac (A. schoenoprasum L.) pripadaju lukovima roda Allium, koji predstavlja jedan od najbrojnijih rodova među monokotilama. Ove ekonomski značajne biljne vrste se koriste u kulinarstvu širom sveta, zbog svog specifičnog mirisa, ukusa i dokazane antioksidativne aktivnosti. U procesima unapređivanja nutritivnih vrednosti i poboljšanja prinosa korišćenjem savremenih biotehnoloških metoda neophodan preduslov je efikasan i pouzdan protokol za regeneraciju in vitro. Pošto takve procedure za regeneraciju in vitro kod šalota i vlašca nisu bile dostupne, osnovni cilj ove disertacije je bio razvijanje efikasnih protokola za indukciju regeneracije pupoljaka/somatskih embriona kod ovih biljnih vrsta.Regeneracija pupoljaka/somatskih embriona je indukovana iz odsečaka korenova šalota i vlašca. Kod obe biljne vrste samo je apikalni deo korena imao regenerativni potencijal pod testiranim eksperimentalnim uslovima, ali se regeneracija odvijala različitim procesima: indirektnom kaulogenezom kod šalota i indirektnom somatskom embriogenezom kod vlašca. Snažan uticaj genotipa na regenerativni potencijal je uočen kod obe biljne vrste. Regenerativni potencijal je testiran na 30 nasumično izabranih linija šalota. Liniju su činili regeneranti dobijeni iz apikalnih odsečaka korenova biljke koja se razvila iz jednog klijanca. Među testiranim linijama je uočena visoka varijabilnost učestalosti regeneracije (0,93-100%) i prosečnog broja pupoljaka po eksplantatu (0,01-20,67). Linija 6 je pokazala najveći kaulogeni i rizogeni potencijal, kao i najbrži regenerativni odgovor. Koristeći apikalne odsečke korenova regenerisanih biljaka linije 6 kao izvor eksplantata, izvršena je optimizacija uslova za indukciju regeneracije. Svetlost, odnos 2,4-dihlorfenoksisirćetne kiseline (2,4-D) i 6-benziladenina (BA) u podlozi i trajanje faze indukcije kalusa su statistički značajno uticali na kaulogeni potencijal ove linije. Optimalna procedura za regeneraciju pupoljaka iz apikalnih odsečaka korenova šalota se sastojala iz pet nedelja kultivacije eksplantata na podlozi za indukciju kalusa sa 5 μM 2,4-D i 5 μM BA, zatim osam nedelja na podlozi za indukciju regeneracije sa 5 μM BA u prisustvu svetlosti i prigustini fluksa od 100 μmol m-2s-1 tokom obe faze...
AB  - Shallot (Allium ascalonicum L.) and chive (A. schoenoprasum L.) belong to the genus Allium, which represents one of the largest genera of the monocotyledonous plants. These economically important plant species have been used worldwide as culinary herbs due to their specific odor, taste and proven potent antioxidative capacity. Crop improvement using modern biotechnological approaches requires an efficient and reliable protocol for in vitro plant regeneration. Since no such protocols for in vitro regeneration of shallot and chive had been available, the main objective of this dissertation was to develop efficient protocols for the induction of bud/somatic embryo regeneration in these plant species.Regeneration of shoots/somatic embryos was induced from root sections of shallot and chive. In both species, only the root-tip sections had the regeneration capacity under the experimental conditions tested, but the regeneration proceeded via different pathways: indirect caulogenesis in shallot and indirect somatic embryogenesis in chive. The strong influence of genotype on regeneration capacity was observed in both species. The regeneration capacity was tested in 30 randomly chosen lines of shallot. A line constitutes the root-tip-derived regenerants originating from a single seed-derived plant. High variability in the frequency of regeneration (0.93-100%) and the mean bud number per explant (0.01-20.67) was observed among these lines. Among them, line 6 exhibited both the highest caulogenic and rizogenic capacity, and the fastest regeneration response. Using the root-tip-derived in vitro regenerated plants of line 6 as an explant source, the regeneration procedure was further optimized. Light intensity, the 2,4-dichlorophenoxyacetic acid (2,4-D)/6-benzyladenine (BA) ratio in callus induction medium and the duration of the callus induction phase significantly affected the caulogenic capacity of this line. Thus, the optimized protocol included a 5-week-long cultivation of the explants on callus induction medium supplemented with 5 μM 2,4-D and 5 μM BA followed by an 8-week-long cultivation on regeneration induction medium containing 5 μM BA, under light with a photosynthetic photon flux density of 100 μmol m-2s-1 during both phases. Using this protocol, a 100% frequency ofregeneration and 18.4 buds per explant were attained in line 6 after 13 weeks of treatment...
PB  - University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Morfogeneza i regeneracija biljaka šalota (Allium ascalonicum L.) i vlašca (A. schoenoprasum L.) in vitro
T1  - In vitro morphogenesis and plant regeneration in shallot (Allium ascalonicum L.) and chive (A. schoenoprasum L.)
SP  - 1
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5953
ER  - 

@phdthesis{
author = "Tubić, Ljiljana",
year = "2016",
abstract = "Šalot (Allium ascalonicum L.) i vlašac (A. schoenoprasum L.) pripadaju lukovima roda Allium, koji predstavlja jedan od najbrojnijih rodova među monokotilama. Ove ekonomski značajne biljne vrste se koriste u kulinarstvu širom sveta, zbog svog specifičnog mirisa, ukusa i dokazane antioksidativne aktivnosti. U procesima unapređivanja nutritivnih vrednosti i poboljšanja prinosa korišćenjem savremenih biotehnoloških metoda neophodan preduslov je efikasan i pouzdan protokol za regeneraciju in vitro. Pošto takve procedure za regeneraciju in vitro kod šalota i vlašca nisu bile dostupne, osnovni cilj ove disertacije je bio razvijanje efikasnih protokola za indukciju regeneracije pupoljaka/somatskih embriona kod ovih biljnih vrsta.Regeneracija pupoljaka/somatskih embriona je indukovana iz odsečaka korenova šalota i vlašca. Kod obe biljne vrste samo je apikalni deo korena imao regenerativni potencijal pod testiranim eksperimentalnim uslovima, ali se regeneracija odvijala različitim procesima: indirektnom kaulogenezom kod šalota i indirektnom somatskom embriogenezom kod vlašca. Snažan uticaj genotipa na regenerativni potencijal je uočen kod obe biljne vrste. Regenerativni potencijal je testiran na 30 nasumično izabranih linija šalota. Liniju su činili regeneranti dobijeni iz apikalnih odsečaka korenova biljke koja se razvila iz jednog klijanca. Među testiranim linijama je uočena visoka varijabilnost učestalosti regeneracije (0,93-100%) i prosečnog broja pupoljaka po eksplantatu (0,01-20,67). Linija 6 je pokazala najveći kaulogeni i rizogeni potencijal, kao i najbrži regenerativni odgovor. Koristeći apikalne odsečke korenova regenerisanih biljaka linije 6 kao izvor eksplantata, izvršena je optimizacija uslova za indukciju regeneracije. Svetlost, odnos 2,4-dihlorfenoksisirćetne kiseline (2,4-D) i 6-benziladenina (BA) u podlozi i trajanje faze indukcije kalusa su statistički značajno uticali na kaulogeni potencijal ove linije. Optimalna procedura za regeneraciju pupoljaka iz apikalnih odsečaka korenova šalota se sastojala iz pet nedelja kultivacije eksplantata na podlozi za indukciju kalusa sa 5 μM 2,4-D i 5 μM BA, zatim osam nedelja na podlozi za indukciju regeneracije sa 5 μM BA u prisustvu svetlosti i prigustini fluksa od 100 μmol m-2s-1 tokom obe faze..., Shallot (Allium ascalonicum L.) and chive (A. schoenoprasum L.) belong to the genus Allium, which represents one of the largest genera of the monocotyledonous plants. These economically important plant species have been used worldwide as culinary herbs due to their specific odor, taste and proven potent antioxidative capacity. Crop improvement using modern biotechnological approaches requires an efficient and reliable protocol for in vitro plant regeneration. Since no such protocols for in vitro regeneration of shallot and chive had been available, the main objective of this dissertation was to develop efficient protocols for the induction of bud/somatic embryo regeneration in these plant species.Regeneration of shoots/somatic embryos was induced from root sections of shallot and chive. In both species, only the root-tip sections had the regeneration capacity under the experimental conditions tested, but the regeneration proceeded via different pathways: indirect caulogenesis in shallot and indirect somatic embryogenesis in chive. The strong influence of genotype on regeneration capacity was observed in both species. The regeneration capacity was tested in 30 randomly chosen lines of shallot. A line constitutes the root-tip-derived regenerants originating from a single seed-derived plant. High variability in the frequency of regeneration (0.93-100%) and the mean bud number per explant (0.01-20.67) was observed among these lines. Among them, line 6 exhibited both the highest caulogenic and rizogenic capacity, and the fastest regeneration response. Using the root-tip-derived in vitro regenerated plants of line 6 as an explant source, the regeneration procedure was further optimized. Light intensity, the 2,4-dichlorophenoxyacetic acid (2,4-D)/6-benzyladenine (BA) ratio in callus induction medium and the duration of the callus induction phase significantly affected the caulogenic capacity of this line. Thus, the optimized protocol included a 5-week-long cultivation of the explants on callus induction medium supplemented with 5 μM 2,4-D and 5 μM BA followed by an 8-week-long cultivation on regeneration induction medium containing 5 μM BA, under light with a photosynthetic photon flux density of 100 μmol m-2s-1 during both phases. Using this protocol, a 100% frequency ofregeneration and 18.4 buds per explant were attained in line 6 after 13 weeks of treatment...",
publisher = "University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Morfogeneza i regeneracija biljaka šalota (Allium ascalonicum L.) i vlašca (A. schoenoprasum L.) in vitro, In vitro morphogenesis and plant regeneration in shallot (Allium ascalonicum L.) and chive (A. schoenoprasum L.)",
pages = "1-189",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5953"
}

Tubić, L.. (2016). Morfogeneza i regeneracija biljaka šalota (Allium ascalonicum L.) i vlašca (A. schoenoprasum L.) in vitro. in University of Belgrade, Faculty of Biology
University of Belgrade, Faculty of Biology., 1-189.
https://hdl.handle.net/21.15107/rcub_nardus_5953

Tubić L. Morfogeneza i regeneracija biljaka šalota (Allium ascalonicum L.) i vlašca (A. schoenoprasum L.) in vitro. in University of Belgrade, Faculty of Biology. 2016;:1-189.
https://hdl.handle.net/21.15107/rcub_nardus_5953 .

Tubić, Ljiljana, "Morfogeneza i regeneracija biljaka šalota (Allium ascalonicum L.) i vlašca (A. schoenoprasum L.) in vitro" in University of Belgrade, Faculty of Biology (2016):1-189,
https://hdl.handle.net/21.15107/rcub_nardus_5953 .

TY  - JOUR
AU  - Tubić, Ljiljana
AU  - Savić, Jelena
AU  - Banjac, Nevena
AU  - Milojević, Jelena
AU  - Janošević, Dušica
AU  - Budimir, Snežana
AU  - Zdravković-Korać, Snežana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4358
AB  - Unlike garlic and onion, the regeneration of chive (Allium schoenoprasum L.), cultivated both for culinary and ornamental purposes, has not been intensively studied. The effects of the eight cytokinins and the plant basal section thickness on regeneration efficiency and subsequent plant growth were studied. Representatives of all cytokinin structural groups: isoprenoide side chain (trans-zeatin) and aromatic side chain (benziladenine, kinetin, meta-topolin) adenine derivatives, and phenylurea derivatives (thidiazuron and N-(2-chloro-4-pyridyl)-N′-phenylurea) at 0, 1, 5 or 10 μM were used. Histological analysis revealed adventitious buds formation from the leaf sheaths’ bases and the basal plate. The highest regeneration frequency (100 %) and the mean bud number per explant (20.0) were achieved with 10 μM thidiazuron (TDZ), and 5 mm-thick basal sections were the most responsive explants. Inferior shoot and root growth characteristics of plants regenerated by this treatment was avoided by exclusion or replacement of 10 μM TDZ with 5 μM kinetin (Kin) after a 4-week bud induction period, without consequences on the regeneration efficiency. In addition, a positive correlation between peroxidase, catalase and superoxide dismutase activity and the regeneration capacity was observed. All antioxidative enzymes activity changed much faster with 10 μM TDZ than with 1 μM Kin, which provoked the weakest regeneration response. Moreover, a unique peroxidase isoform was observed only in TDZ-treated explants after 3rd day of treatment. This work is useful for genetic engineering and virus-free plant production advancement, and for the knowledge expansion regarding the role of antioxidative enzymes in plant organogenesis.
PB  - Dordrecht: Springer
T2  - Plant Cell Tissue and Organ Culture
T1  - Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)
IS  - 1
VL  - 124
DO  - 10.1007/s11240-015-0869-1
SP  - 1
EP  - 14
ER  - 

@article{
author = "Tubić, Ljiljana and Savić, Jelena and Banjac, Nevena and Milojević, Jelena and Janošević, Dušica and Budimir, Snežana and Zdravković-Korać, Snežana",
year = "2016",
abstract = "Unlike garlic and onion, the regeneration of chive (Allium schoenoprasum L.), cultivated both for culinary and ornamental purposes, has not been intensively studied. The effects of the eight cytokinins and the plant basal section thickness on regeneration efficiency and subsequent plant growth were studied. Representatives of all cytokinin structural groups: isoprenoide side chain (trans-zeatin) and aromatic side chain (benziladenine, kinetin, meta-topolin) adenine derivatives, and phenylurea derivatives (thidiazuron and N-(2-chloro-4-pyridyl)-N′-phenylurea) at 0, 1, 5 or 10 μM were used. Histological analysis revealed adventitious buds formation from the leaf sheaths’ bases and the basal plate. The highest regeneration frequency (100 %) and the mean bud number per explant (20.0) were achieved with 10 μM thidiazuron (TDZ), and 5 mm-thick basal sections were the most responsive explants. Inferior shoot and root growth characteristics of plants regenerated by this treatment was avoided by exclusion or replacement of 10 μM TDZ with 5 μM kinetin (Kin) after a 4-week bud induction period, without consequences on the regeneration efficiency. In addition, a positive correlation between peroxidase, catalase and superoxide dismutase activity and the regeneration capacity was observed. All antioxidative enzymes activity changed much faster with 10 μM TDZ than with 1 μM Kin, which provoked the weakest regeneration response. Moreover, a unique peroxidase isoform was observed only in TDZ-treated explants after 3rd day of treatment. This work is useful for genetic engineering and virus-free plant production advancement, and for the knowledge expansion regarding the role of antioxidative enzymes in plant organogenesis.",
publisher = "Dordrecht: Springer",
journal = "Plant Cell Tissue and Organ Culture",
title = "Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)",
number = "1",
volume = "124",
doi = "10.1007/s11240-015-0869-1",
pages = "1-14"
}

Tubić, L., Savić, J., Banjac, N., Milojević, J., Janošević, D., Budimir, S.,& Zdravković-Korać, S.. (2016). Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.). in Plant Cell Tissue and Organ Culture
Dordrecht: Springer., 124(1), 1-14.
https://doi.org/10.1007/s11240-015-0869-1

Tubić L, Savić J, Banjac N, Milojević J, Janošević D, Budimir S, Zdravković-Korać S. Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.). in Plant Cell Tissue and Organ Culture. 2016;124(1):1-14.
doi:10.1007/s11240-015-0869-1 .

Tubić, Ljiljana, Savić, Jelena, Banjac, Nevena, Milojević, Jelena, Janošević, Dušica, Budimir, Snežana, Zdravković-Korać, Snežana, "Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)" in Plant Cell Tissue and Organ Culture, 124, no. 1 (2016):1-14,
https://doi.org/10.1007/s11240-015-0869-1 . .

TY  - CONF
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6677
AB  - During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions, resulting in diversified phenotypes. We were interested in the response of plants towards changing environmental parameters, to light in particular. For this study we selected genotypes of Iris variegata that experienced different light conditions in their habitats: a) genotypes situated along the top and slope of a dune, where it coexisted with annual and perennial herbs and low shrubs; b) genotypes inhabited the woodland understories. We grew I.variegata genotypes in two experimental light treatments (high and low) and tested differences between spring, summer and autumn as well as between two successive years. The plants responses were analysed at different levels of biological information and it was revealed that dissimilar light conditions markedly affected all aspects of plant anatomy and  physiology. Repeated measures model ANOVA detected a significant effect of year, habitat and genotype in most analysed traits. Models of changes during growing seasons were treatment- and year-specific. We also present results from an on-going study which aims to assess occurrence patterns of 
phenotypic plasticity based on system-level analysis of the same sample that includes metabolic profiling approach combined with robust statistical analysis.
PB  - Université de Lausanne, Départment d’écologie et évolution
C3  - Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland
T1  - Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6677
ER  - 

@conference{
author = "Živković, Uroš and Avramov, Stevan and Barišić Klisarić, Nataša and Miljković, Danijela and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2015",
abstract = "During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions, resulting in diversified phenotypes. We were interested in the response of plants towards changing environmental parameters, to light in particular. For this study we selected genotypes of Iris variegata that experienced different light conditions in their habitats: a) genotypes situated along the top and slope of a dune, where it coexisted with annual and perennial herbs and low shrubs; b) genotypes inhabited the woodland understories. We grew I.variegata genotypes in two experimental light treatments (high and low) and tested differences between spring, summer and autumn as well as between two successive years. The plants responses were analysed at different levels of biological information and it was revealed that dissimilar light conditions markedly affected all aspects of plant anatomy and  physiology. Repeated measures model ANOVA detected a significant effect of year, habitat and genotype in most analysed traits. Models of changes during growing seasons were treatment- and year-specific. We also present results from an on-going study which aims to assess occurrence patterns of 
phenotypic plasticity based on system-level analysis of the same sample that includes metabolic profiling approach combined with robust statistical analysis.",
publisher = "Université de Lausanne, Départment d’écologie et évolution",
journal = "Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland",
title = "Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6677"
}

Živković, U., Avramov, S., Barišić Klisarić, N., Miljković, D., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2015). Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions. in Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland
Université de Lausanne, Départment d’écologie et évolution..
https://hdl.handle.net/21.15107/rcub_ibiss_6677

Živković U, Avramov S, Barišić Klisarić N, Miljković D, Tubić L, Mišić D, Šiler B, Tarasjev A. Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions. in Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland. 2015;.
https://hdl.handle.net/21.15107/rcub_ibiss_6677 .

Živković, Uroš, Avramov, Stevan, Barišić Klisarić, Nataša, Miljković, Danijela, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions" in Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland (2015),
https://hdl.handle.net/21.15107/rcub_ibiss_6677 .

TY  - JOUR
AU  - Tubić, Ljiljana
AU  - Anackov, Goran
AU  - Milojević, Jelena
AU  - Ghalawenji, Nabil
AU  - Banjac, Nevena
AU  - Igić, Ružica
AU  - Zdravković-Korać, Snežana
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2198
AB  - This study assessed the shoot regeneration capacity of root-tips
   isolated from single seed-derived individual plants, obtained from a
   wild, open-pollinated micropopulation of shallot (Allium ascalonicum).
   Considerable variation was observed in the regeneration capacities of
   individual lines, ranging from 0.93 to 100 \%, and a mean bud number per
   root explant between 0.09 and 20.67. One line was found to be superior,
   and was chosen for protocol optimization, focusing on the 2,4-D/BA
   ratio, duration of the CI phase and light conditions. The application of
   the optimized protocol to other lines, selected for their variable
   regeneration capacities, enhanced the process of regeneration and
   shortened the time required to obtain healthy plantlets, even in
   inferior lines. However, highly responsive lines remained superior,
   indicating that differences at the individual level must not be
   overlooked. The conditions employed in this study may serve as a base to
   facilitate the application of molecular breeding methods in shallot.
T2  - Plant Cell Tissue and Organ Culture
T1  - High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure
IS  - 1
VL  - 118
DO  - 10.1007/s11240-014-0465-9
SP  - 101
EP  - 110
ER  - 

@article{
author = "Tubić, Ljiljana and Anackov, Goran and Milojević, Jelena and Ghalawenji, Nabil and Banjac, Nevena and Igić, Ružica and Zdravković-Korać, Snežana",
year = "2014",
abstract = "This study assessed the shoot regeneration capacity of root-tips
   isolated from single seed-derived individual plants, obtained from a
   wild, open-pollinated micropopulation of shallot (Allium ascalonicum).
   Considerable variation was observed in the regeneration capacities of
   individual lines, ranging from 0.93 to 100 \%, and a mean bud number per
   root explant between 0.09 and 20.67. One line was found to be superior,
   and was chosen for protocol optimization, focusing on the 2,4-D/BA
   ratio, duration of the CI phase and light conditions. The application of
   the optimized protocol to other lines, selected for their variable
   regeneration capacities, enhanced the process of regeneration and
   shortened the time required to obtain healthy plantlets, even in
   inferior lines. However, highly responsive lines remained superior,
   indicating that differences at the individual level must not be
   overlooked. The conditions employed in this study may serve as a base to
   facilitate the application of molecular breeding methods in shallot.",
journal = "Plant Cell Tissue and Organ Culture",
title = "High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure",
number = "1",
volume = "118",
doi = "10.1007/s11240-014-0465-9",
pages = "101-110"
}

Tubić, L., Anackov, G., Milojević, J., Ghalawenji, N., Banjac, N., Igić, R.,& Zdravković-Korać, S.. (2014). High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure. in Plant Cell Tissue and Organ Culture, 118(1), 101-110.
https://doi.org/10.1007/s11240-014-0465-9

Tubić L, Anackov G, Milojević J, Ghalawenji N, Banjac N, Igić R, Zdravković-Korać S. High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure. in Plant Cell Tissue and Organ Culture. 2014;118(1):101-110.
doi:10.1007/s11240-014-0465-9 .

Tubić, Ljiljana, Anackov, Goran, Milojević, Jelena, Ghalawenji, Nabil, Banjac, Nevena, Igić, Ružica, Zdravković-Korać, Snežana, "High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure" in Plant Cell Tissue and Organ Culture, 118, no. 1 (2014):101-110,
https://doi.org/10.1007/s11240-014-0465-9 . .