TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Popović, Dušanka
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6507
AB  - The use of anticoagulant rodenticides (ARs) is one of the most commonly employed management methods for
pest rodents. ARs compete with vitamin K (VK) required for the synthesis of blood clotting factors in the liver,
resulting in inhibition of blood coagulation and often animal death due to hemorrhage. Besides rodents (target
species), ARs may affect non-target animal species and humans. Out of hemostasis disturbance, the effects of ARs
may be related to the inhibition of proteins that require VK for their synthesis but are not involved in the
coagulation process, to their direct cytotoxicity, and their pro-oxidant/proinflammatory activity. A survey of the
cellular and molecular mechanisms of these sublethal/asymptomatic AR effects is given in this review. Data from
field, clinical, and experimental studies are presented. Knowledge of these mechanisms might improve hazard
characterization and identification of potential ecotoxicological risks associated with ARs, contributing to a safer
use of these chemicals.
PB  - New York, NY : Elsevier Science
T2  - Comparative Biochemistry and Physiology, Part C
T1  - Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance
VL  - 277
DO  - 10.1016/j.cbpc.2024.109841
SP  - 109841
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Popović, Dušanka and Kataranovski, Dragan and Kataranovski, Milena and Mirkov, Ivana",
year = "2024",
abstract = "The use of anticoagulant rodenticides (ARs) is one of the most commonly employed management methods for
pest rodents. ARs compete with vitamin K (VK) required for the synthesis of blood clotting factors in the liver,
resulting in inhibition of blood coagulation and often animal death due to hemorrhage. Besides rodents (target
species), ARs may affect non-target animal species and humans. Out of hemostasis disturbance, the effects of ARs
may be related to the inhibition of proteins that require VK for their synthesis but are not involved in the
coagulation process, to their direct cytotoxicity, and their pro-oxidant/proinflammatory activity. A survey of the
cellular and molecular mechanisms of these sublethal/asymptomatic AR effects is given in this review. Data from
field, clinical, and experimental studies are presented. Knowledge of these mechanisms might improve hazard
characterization and identification of potential ecotoxicological risks associated with ARs, contributing to a safer
use of these chemicals.",
publisher = "New York, NY : Elsevier Science",
journal = "Comparative Biochemistry and Physiology, Part C",
title = "Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance",
volume = "277",
doi = "10.1016/j.cbpc.2024.109841",
pages = "109841"
}

Popov Aleksandrov, A., Tucović, D., Kulaš, J., Popović, D., Kataranovski, D., Kataranovski, M.,& Mirkov, I.. (2024). Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance. in Comparative Biochemistry and Physiology, Part C
New York, NY : Elsevier Science., 277, 109841.
https://doi.org/10.1016/j.cbpc.2024.109841

Popov Aleksandrov A, Tucović D, Kulaš J, Popović D, Kataranovski D, Kataranovski M, Mirkov I. Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance. in Comparative Biochemistry and Physiology, Part C. 2024;277:109841.
doi:10.1016/j.cbpc.2024.109841 .

Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Popović, Dušanka, Kataranovski, Dragan, Kataranovski, Milena, Mirkov, Ivana, "Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance" in Comparative Biochemistry and Physiology, Part C, 277 (2024):109841,
https://doi.org/10.1016/j.cbpc.2024.109841 . .

TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Tolinački, Maja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6084
AB  - While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.
PB  - American Society for Microbiology
T2  - Microbiology Spectrum
T1  - Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition
IS  - 5
VL  - 11
DO  - 10.1128/spectrum.01990-23
SP  - e0199023
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Malešević, Anastasija and Glamočlija, Jasmina and Brdarić, Emilija and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Tolinački, Maja",
year = "2023",
abstract = "While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.",
publisher = "American Society for Microbiology",
journal = "Microbiology Spectrum",
title = "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition",
number = "5",
volume = "11",
doi = "10.1128/spectrum.01990-23",
pages = "e0199023"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Malešević, A., Glamočlija, J., Brdarić, E., Soković Bajić, S., Golić, N., Mirkov, I.,& Tolinački, M.. (2023). Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum
American Society for Microbiology., 11(5), e0199023.
https://doi.org/10.1128/spectrum.01990-23

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Malešević A, Glamočlija J, Brdarić E, Soković Bajić S, Golić N, Mirkov I, Tolinački M. Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum. 2023;11(5):e0199023.
doi:10.1128/spectrum.01990-23 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Malešević, Anastasija, Glamočlija, Jasmina, Brdarić, Emilija, Soković Bajić, Svetlana, Golić, Nataša, Mirkov, Ivana, Tolinački, Maja, "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition" in Microbiology Spectrum, 11, no. 5 (2023):e0199023,
https://doi.org/10.1128/spectrum.01990-23 . .

TY  - CONF
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Soković Bajić, Svetlana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6113
AB  - Crevna mikrobiota ima važnu ulogu u održavanju homeostaze koju ostvaruje preko interakcija sa imunskim
sistemom i gastrointestinalnim traktom. Toksične supstance ostvaruju direktan štetni efekat na sastav
i funkcionalnost crevne mikrobiote. Kadmijum (Cd) je metal sa izraženom toksičnošću, koji se povezuje
sa brojnim oboljenjima. U našoj studiji je predložen mehanizam zaštite koji bi se ostvario posredstvom
bakterijskih egzopolisaharida (EPS) izolovanih iz soja Lactiplantibacillus plantarum BGAN8, a bazira se
na visokom afinitetu adsorpcije kadmijumovih jona. Ciljevi rada su bili utvrđivanje efekata produženog
delovanja kadmijuma na sastav crevne mikrobiote Dark agouti (DA) pacova, kao i izučavanje efekta
EPS-AN8 na ublažavanje takvih štetnih efekata.
Pacovi su kroz vodu unosili nižu (5 ppm) i višu (50 ppm) dozu kadmijuma, dok je EPS-AN8 (100 μg/ml) dat
kroz hranu za životinje. Totalna DNK je izolovana iz lumena duodenuma i 16S rDNK amplikon je sekvenciran
strategijom uparenih krajeva na Illumina NovaSeq platformi. Dodatno, praćene su promene u
inflamaciji i oksidativnom stresu homogenata duodenuma. Kadmijum je favorizovao rast oportunističkih
patogenih bakterija poput pripadnika rodova Blautia, Prevotella, Alloprevotela, Bacteroides, dok je
relativna zastupljenost laktobacila bila značajno smanjena. Obe doze kadmijuma su stimulisale produkciju
proinflamatornih citokina (IL-1β, TNF-α i IFN-γ) i povisile nivo oksidativnog stresa (MDA, CAT i
GST). Oralni unos EPS-AN8 je značajno ublažio sve navedene promene. Oralna izloženost kadmijumu
uzrokuje nedvosmislene promene u sastavu mikrobiote, koje mogu biti praćene i pojačanim nivoima
inflamacije i oksidativnog stresa. Nasuprot tome, istovremeni unos EPS-AN8 redukuje pojavu takvih
promena.
AB  - The gut microbiota has important role in maintaining homeostasis via its effect on the immune system
and healthy gastrointestinal tract. Environmental toxicants directly influenced the changes in gut microbiota
composition. Cadmium (Cd) is a hazard, world widely distributed metal. We have proposed
the putative mechanism of protection against Cd harmful effect in the organism by using exopolysaccharides
derived from Lactiplantibacillus plantarum BGAN8 (EPS-AN8) which has great ability to sequester Cd ions.
Aims of our study were to examine Cd related changes in the gut microbiota of Dark agouti (DA) rats after
prolonged oral exposure (30 days) to this metal and to determine impact of EPS-AN8 in preventing
those changes.
Rats were given via water lower (5 ppm) and higher (50 ppm) Cd’s dose. In paralel, EPS-AN8 was administrated
through food (100 μg/ml). Total DNA from duodenum was isolated and PCR amplicon for 16SrRNA was
sequenced on Illumina NovaSeq paired end platform. Moreover, we have followed changes in
oxidative stress and inflammation in duodenum. Cd favored growth of opportunistic pathogen bacteria
genera (Blautia, Prevotella, Alloprevotela, Bacteroides) while the relative abundance of lactobacilli was
decreased. The both doses of Cd increased production of inflammatory cytokines (IL-1β, TNF-α, and
IFN-γ). Also, parameters of oxidative stress were significantly upregulated (MDA, CAT, and GST).
All the mentioned changes were alleviated by EPS-AN8. Oral exposure to Cd lead to inevitable changes
in the microbiota structure followed by inflammation and oxidative stress of surrounding tissue, while
administration of EPS-AN8 reversed those changes.
PB  - Belgrade: Serbian Society of Toxicology
C3  - Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
T1  - Gut microbiota as a target of toxic compounds
T1  - Crevna mikrobiota kao osetljiva meta delovanja toksičnih supstanci
SP  - 92
EP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6113
ER  - 

@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Kulaš, Jelena and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Crevna mikrobiota ima važnu ulogu u održavanju homeostaze koju ostvaruje preko interakcija sa imunskim
sistemom i gastrointestinalnim traktom. Toksične supstance ostvaruju direktan štetni efekat na sastav
i funkcionalnost crevne mikrobiote. Kadmijum (Cd) je metal sa izraženom toksičnošću, koji se povezuje
sa brojnim oboljenjima. U našoj studiji je predložen mehanizam zaštite koji bi se ostvario posredstvom
bakterijskih egzopolisaharida (EPS) izolovanih iz soja Lactiplantibacillus plantarum BGAN8, a bazira se
na visokom afinitetu adsorpcije kadmijumovih jona. Ciljevi rada su bili utvrđivanje efekata produženog
delovanja kadmijuma na sastav crevne mikrobiote Dark agouti (DA) pacova, kao i izučavanje efekta
EPS-AN8 na ublažavanje takvih štetnih efekata.
Pacovi su kroz vodu unosili nižu (5 ppm) i višu (50 ppm) dozu kadmijuma, dok je EPS-AN8 (100 μg/ml) dat
kroz hranu za životinje. Totalna DNK je izolovana iz lumena duodenuma i 16S rDNK amplikon je sekvenciran
strategijom uparenih krajeva na Illumina NovaSeq platformi. Dodatno, praćene su promene u
inflamaciji i oksidativnom stresu homogenata duodenuma. Kadmijum je favorizovao rast oportunističkih
patogenih bakterija poput pripadnika rodova Blautia, Prevotella, Alloprevotela, Bacteroides, dok je
relativna zastupljenost laktobacila bila značajno smanjena. Obe doze kadmijuma su stimulisale produkciju
proinflamatornih citokina (IL-1β, TNF-α i IFN-γ) i povisile nivo oksidativnog stresa (MDA, CAT i
GST). Oralni unos EPS-AN8 je značajno ublažio sve navedene promene. Oralna izloženost kadmijumu
uzrokuje nedvosmislene promene u sastavu mikrobiote, koje mogu biti praćene i pojačanim nivoima
inflamacije i oksidativnog stresa. Nasuprot tome, istovremeni unos EPS-AN8 redukuje pojavu takvih
promena., The gut microbiota has important role in maintaining homeostasis via its effect on the immune system
and healthy gastrointestinal tract. Environmental toxicants directly influenced the changes in gut microbiota
composition. Cadmium (Cd) is a hazard, world widely distributed metal. We have proposed
the putative mechanism of protection against Cd harmful effect in the organism by using exopolysaccharides
derived from Lactiplantibacillus plantarum BGAN8 (EPS-AN8) which has great ability to sequester Cd ions.
Aims of our study were to examine Cd related changes in the gut microbiota of Dark agouti (DA) rats after
prolonged oral exposure (30 days) to this metal and to determine impact of EPS-AN8 in preventing
those changes.
Rats were given via water lower (5 ppm) and higher (50 ppm) Cd’s dose. In paralel, EPS-AN8 was administrated
through food (100 μg/ml). Total DNA from duodenum was isolated and PCR amplicon for 16SrRNA was
sequenced on Illumina NovaSeq paired end platform. Moreover, we have followed changes in
oxidative stress and inflammation in duodenum. Cd favored growth of opportunistic pathogen bacteria
genera (Blautia, Prevotella, Alloprevotela, Bacteroides) while the relative abundance of lactobacilli was
decreased. The both doses of Cd increased production of inflammatory cytokines (IL-1β, TNF-α, and
IFN-γ). Also, parameters of oxidative stress were significantly upregulated (MDA, CAT, and GST).
All the mentioned changes were alleviated by EPS-AN8. Oral exposure to Cd lead to inevitable changes
in the microbiota structure followed by inflammation and oxidative stress of surrounding tissue, while
administration of EPS-AN8 reversed those changes.",
publisher = "Belgrade: Serbian Society of Toxicology",
journal = "Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia",
title = "Gut microbiota as a target of toxic compounds, Crevna mikrobiota kao osetljiva meta delovanja toksičnih supstanci",
pages = "92-93",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6113"
}

Brdarić, E., Popović, D., Kulaš, J., Soković Bajić, S., Golić, N., Mirkov, I.,& Živković, M.. (2023). Gut microbiota as a target of toxic compounds. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
Belgrade: Serbian Society of Toxicology., 92-93.
https://hdl.handle.net/21.15107/rcub_ibiss_6113

Brdarić E, Popović D, Kulaš J, Soković Bajić S, Golić N, Mirkov I, Živković M. Gut microbiota as a target of toxic compounds. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia. 2023;:92-93.
https://hdl.handle.net/21.15107/rcub_ibiss_6113 .

Brdarić, Emilija, Popović, Dušanka, Kulaš, Jelena, Soković Bajić, Svetlana, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Gut microbiota as a target of toxic compounds" in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia (2023):92-93,
https://hdl.handle.net/21.15107/rcub_ibiss_6113 .

TY  - JOUR
AU  - Popović, Dušanka
AU  - Malešević, Anastasija
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6475
AB  - Bacterial microbiota of the gastrointestinal tract is known to prevent the invasion of
pathogenic microorganisms and regulate intestinal permeability, digestion, metabolism, and
immune response. It affects function, homeostasis, and disease outcomes in the gastrointestinal
tract and extra-intestinal sites such as the lungs. This review summarizes the currently available
knowledge regarding the gut-lung axis. The association of bacterial composition and/or dysbiosis
in the gut with asthma, chronic obstructive lung disease, cystic fibrosis, recurrent respiratory tract
infections, and lung cancer in humans is highlighted, as well as data obtained from animal models
of pulmonary inflammation, which indicated that modulation of immune system activity lies at
the base of this interaction. Additionally, the potential use of prebiotics, probiotics, and postbiotics
in the treatment of lung inflammation is presented.
AB  - Poznato je da bakterije prisutne u gastrointestinalnom traktu imaju ulogu u sprečavanju
invazije patogenih mikroorganizama, regulaciji propustljivosti creva, varenju hrane, metabolizmu
i imunskom odgovoru. Ove bakterije utiču na funkciju, održavanje homeostaze i ishod bolesti
kako u gastrointestinalnom traktu, tako i u udaljenim organima kao što su pluća. Ovaj pregledni
rad sumira trenutno dostupna znanja o osi creva-pluća. Prikazana je veza između bakterijskog
sastava i/ili disbioze u crevima sa različitim bolestima kod ljudi kao što su astma, hronična
opstruktivna bolest pluća, cistična fibroza, rekurentne infekcije respiratornog trakta i karcinom
pluća, kao i podaci dobijeni u životinjskim modelima inflamacije pluća koji su pokazali da
modulacija aktivnosti imunskog sistema leži u osnovi ove interakcije. Potencijalna upotreba
prebiotika, probiotika i postbiotika u terapiji inflamacije u plućima je takođe prikazana.
PB  - Beograd: Savez farmaceutskih udruženja Srbije
PB  - Beograd: Farmaceutski fakultet Univerziteta u Beogradu
T2  - Arhiv za farmaciju
T1  - Impact of gut microbiota on immune reactions relevant to lung pathologies
IS  - 6
VL  - 73
DO  - 10.5937/arhfarm73-46387
SP  - 463
EP  - 483
ER  - 

@article{
author = "Popović, Dušanka and Malešević, Anastasija and Tucović, Dina and Kulaš, Jelena and Popov Aleksandrov, Aleksandra and Mirkov, Ivana",
year = "2023",
abstract = "Bacterial microbiota of the gastrointestinal tract is known to prevent the invasion of
pathogenic microorganisms and regulate intestinal permeability, digestion, metabolism, and
immune response. It affects function, homeostasis, and disease outcomes in the gastrointestinal
tract and extra-intestinal sites such as the lungs. This review summarizes the currently available
knowledge regarding the gut-lung axis. The association of bacterial composition and/or dysbiosis
in the gut with asthma, chronic obstructive lung disease, cystic fibrosis, recurrent respiratory tract
infections, and lung cancer in humans is highlighted, as well as data obtained from animal models
of pulmonary inflammation, which indicated that modulation of immune system activity lies at
the base of this interaction. Additionally, the potential use of prebiotics, probiotics, and postbiotics
in the treatment of lung inflammation is presented., Poznato je da bakterije prisutne u gastrointestinalnom traktu imaju ulogu u sprečavanju
invazije patogenih mikroorganizama, regulaciji propustljivosti creva, varenju hrane, metabolizmu
i imunskom odgovoru. Ove bakterije utiču na funkciju, održavanje homeostaze i ishod bolesti
kako u gastrointestinalnom traktu, tako i u udaljenim organima kao što su pluća. Ovaj pregledni
rad sumira trenutno dostupna znanja o osi creva-pluća. Prikazana je veza između bakterijskog
sastava i/ili disbioze u crevima sa različitim bolestima kod ljudi kao što su astma, hronična
opstruktivna bolest pluća, cistična fibroza, rekurentne infekcije respiratornog trakta i karcinom
pluća, kao i podaci dobijeni u životinjskim modelima inflamacije pluća koji su pokazali da
modulacija aktivnosti imunskog sistema leži u osnovi ove interakcije. Potencijalna upotreba
prebiotika, probiotika i postbiotika u terapiji inflamacije u plućima je takođe prikazana.",
publisher = "Beograd: Savez farmaceutskih udruženja Srbije, Beograd: Farmaceutski fakultet Univerziteta u Beogradu",
journal = "Arhiv za farmaciju",
title = "Impact of gut microbiota on immune reactions relevant to lung pathologies",
number = "6",
volume = "73",
doi = "10.5937/arhfarm73-46387",
pages = "463-483"
}

Popović, D., Malešević, A., Tucović, D., Kulaš, J., Popov Aleksandrov, A.,& Mirkov, I.. (2023). Impact of gut microbiota on immune reactions relevant to lung pathologies. in Arhiv za farmaciju
Beograd: Savez farmaceutskih udruženja Srbije., 73(6), 463-483.
https://doi.org/10.5937/arhfarm73-46387

Popović D, Malešević A, Tucović D, Kulaš J, Popov Aleksandrov A, Mirkov I. Impact of gut microbiota on immune reactions relevant to lung pathologies. in Arhiv za farmaciju. 2023;73(6):463-483.
doi:10.5937/arhfarm73-46387 .

Popović, Dušanka, Malešević, Anastasija, Tucović, Dina, Kulaš, Jelena, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, "Impact of gut microbiota on immune reactions relevant to lung pathologies" in Arhiv za farmaciju, 73, no. 6 (2023):463-483,
https://doi.org/10.5937/arhfarm73-46387 . .

TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Tolinacki, Maja
AU  - Golić, Nataša
AU  - Mirkov, Ivana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6162
AB  - Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated
bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota
composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus
Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA
and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized
by increased alpha diversity indices and unchanged beta diversity. The relative abundance of
genera and species was increased in DA but decreased in AO rats during infection. Changes in lung
microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate
response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat
strains differentially susceptible to a subsequent inflammatory insult.
PB  - Paris: Elsevier Masson SAS
T2  - Microbes and Infection
T1  - Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats
DO  - 10.1016/j.micinf.2023.105186
SP  - 105186
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Glamočlija, Jasmina and Soković Bajić, Svetlana and Tolinacki, Maja and Golić, Nataša and Mirkov, Ivana",
year = "2023",
abstract = "Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated
bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota
composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus
Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA
and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized
by increased alpha diversity indices and unchanged beta diversity. The relative abundance of
genera and species was increased in DA but decreased in AO rats during infection. Changes in lung
microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate
response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat
strains differentially susceptible to a subsequent inflammatory insult.",
publisher = "Paris: Elsevier Masson SAS",
journal = "Microbes and Infection",
title = "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats",
doi = "10.1016/j.micinf.2023.105186",
pages = "105186"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Glamočlija, J., Soković Bajić, S., Tolinacki, M., Golić, N.,& Mirkov, I.. (2023). Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection
Paris: Elsevier Masson SAS., 105186.
https://doi.org/10.1016/j.micinf.2023.105186

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Glamočlija J, Soković Bajić S, Tolinacki M, Golić N, Mirkov I. Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection. 2023;:105186.
doi:10.1016/j.micinf.2023.105186 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Glamočlija, Jasmina, Soković Bajić, Svetlana, Tolinacki, Maja, Golić, Nataša, Mirkov, Ivana, "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats" in Microbes and Infection (2023):105186,
https://doi.org/10.1016/j.micinf.2023.105186 . .

TY  - CONF
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6125
AB  - Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом.
AB  - Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
T1  - Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)
T1  - Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6125
ER  - 

@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом., Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia",
title = "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II), Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6125"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
Belgrade: Serbian Academy of Sciences and Arts..
https://hdl.handle.net/21.15107/rcub_ibiss_6125

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6125 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)" in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6125 .

TY  - CONF
AU  - Popović, Dušanka
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Brdarić, Emilija
AU  - Tolinački, Maja
AU  - Golić, Nataša
PY  - 2023
UR  - https://probiotics-prebiotics-newfood.com/wp-content/uploads/2023/09/Pogramme-web-07_09_2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6166
AB  - Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.
PB  - Roma: EMEC
C3  - 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
T1  - The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6166
ER  - 

@conference{
author = "Popović, Dušanka and Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Malešević, Anastasija and Glamočlija, Jasmina and Soković Bajić, Svetlana and Brdarić, Emilija and Tolinački, Maja and Golić, Nataša",
year = "2023",
abstract = "Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.",
publisher = "Roma: EMEC",
journal = "12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy",
title = "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6166"
}

Popović, D., Mirkov, I., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Malešević, A., Glamočlija, J., Soković Bajić, S., Brdarić, E., Tolinački, M.,& Golić, N.. (2023). The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
Roma: EMEC., 90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166

Popović D, Mirkov I, Popov Aleksandrov A, Tucović D, Kulaš J, Malešević A, Glamočlija J, Soković Bajić S, Brdarić E, Tolinački M, Golić N. The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy. 2023;:90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .

Popović, Dušanka, Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Malešević, Anastasija, Glamočlija, Jasmina, Soković Bajić, Svetlana, Brdarić, Emilija, Tolinački, Maja, Golić, Nataša, "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats" in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy (2023):90-90,
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .

TY  - JOUR
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5479
AB  - Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led
to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas
the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on
the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation
(IL-1 , TNF- , and IFN-
) in the duodenum. Moreover, micrographs of the duodenum were in line
with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used
16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the
microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly
favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those
changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting
this toxic environmental pollutant.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats
IS  - 3
VL  - 24
DO  - 10.3390/ijms24032845
SP  - 2845
ER  - 

@article{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led
to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas
the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on
the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation
(IL-1 , TNF- , and IFN-
) in the duodenum. Moreover, micrographs of the duodenum were in line
with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used
16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the
microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly
favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those
changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting
this toxic environmental pollutant.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats",
number = "3",
volume = "24",
doi = "10.3390/ijms24032845",
pages = "2845"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences
Basel: MDPI., 24(3), 2845.
https://doi.org/10.3390/ijms24032845

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences. 2023;24(3):2845.
doi:10.3390/ijms24032845 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats" in International Journal of Molecular Sciences, 24, no. 3 (2023):2845,
https://doi.org/10.3390/ijms24032845 . .

TY  - JOUR
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Mirkov, Ivana
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Despotović, Marta
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5301
AB  - Objective The effect of oral cadmium (Cd) intake to influence contact skin allergies was examined,
since it is known that Cd is a heavy metal that affects many tissues, including the skin, in which it
disturbs homeostasis, thus resulting in inflammation and injury.
Methods Male rats were evoked with experimental contact hypersensitivity reaction (CHS) to hapten
dinitrochlorobenzene (DNCB), after prolonged (30 day) oral exposure to an environmentally relevant Cd
dose (5 ppm). The ear cell population was analyzed with flow cytometry. Cytokine production by ear
skin cells and the activity of skin-draining lymph node (DLN) cells were measured using enzyme-linked
immunosorbent assay (ELISA).
Results Orally acquired Cd (5 ppm) increased CHS intensity only in Dark Agouti (DA) rats by affecting
inflammatory responses in both the sensitization (an increase of IFN-γ and IL-17 cytokine production)
and challenge (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine production)
phases. An increased CHS reaction was seen in Albino Oxford (AO) rats only at a high Cd dose (50 ppm),
during the challenge phase (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine
production).
Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals
such as DNCB.
PB  - Beijing: Chinese Center for Disease Control and Prevention
T2  - Biomedical and Environmental Sciences
T1  - Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats
IS  - 11
VL  - 35
DO  - 10.3967/bes2022.132
SP  - 1038
EP  - 1050
ER  - 

@article{
author = "Tucović, Dina and Kulaš, Jelena and Mirkov, Ivana and Popović, Dušanka and Zolotarevski, Lidija and Despotović, Marta and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2022",
abstract = "Objective The effect of oral cadmium (Cd) intake to influence contact skin allergies was examined,
since it is known that Cd is a heavy metal that affects many tissues, including the skin, in which it
disturbs homeostasis, thus resulting in inflammation and injury.
Methods Male rats were evoked with experimental contact hypersensitivity reaction (CHS) to hapten
dinitrochlorobenzene (DNCB), after prolonged (30 day) oral exposure to an environmentally relevant Cd
dose (5 ppm). The ear cell population was analyzed with flow cytometry. Cytokine production by ear
skin cells and the activity of skin-draining lymph node (DLN) cells were measured using enzyme-linked
immunosorbent assay (ELISA).
Results Orally acquired Cd (5 ppm) increased CHS intensity only in Dark Agouti (DA) rats by affecting
inflammatory responses in both the sensitization (an increase of IFN-γ and IL-17 cytokine production)
and challenge (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine production)
phases. An increased CHS reaction was seen in Albino Oxford (AO) rats only at a high Cd dose (50 ppm),
during the challenge phase (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine
production).
Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals
such as DNCB.",
publisher = "Beijing: Chinese Center for Disease Control and Prevention",
journal = "Biomedical and Environmental Sciences",
title = "Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats",
number = "11",
volume = "35",
doi = "10.3967/bes2022.132",
pages = "1038-1050"
}

Tucović, D., Kulaš, J., Mirkov, I., Popović, D., Zolotarevski, L., Despotović, M., Kataranovski, M.,& Popov Aleksandrov, A.. (2022). Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats. in Biomedical and Environmental Sciences
Beijing: Chinese Center for Disease Control and Prevention., 35(11), 1038-1050.
https://doi.org/10.3967/bes2022.132

Tucović D, Kulaš J, Mirkov I, Popović D, Zolotarevski L, Despotović M, Kataranovski M, Popov Aleksandrov A. Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats. in Biomedical and Environmental Sciences. 2022;35(11):1038-1050.
doi:10.3967/bes2022.132 .

Tucović, Dina, Kulaš, Jelena, Mirkov, Ivana, Popović, Dušanka, Zolotarevski, Lidija, Despotović, Marta, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats" in Biomedical and Environmental Sciences, 35, no. 11 (2022):1038-1050,
https://doi.org/10.3967/bes2022.132 . .

TY  - CONF
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Ninkov, Marina
AU  - Popović, Dušanka
AU  - Malešević, Anastasija
AU  - Kataranovski, Milena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5141
AB  - Имунски систем је осетљива мета токсичног деловања загађивача из спољашње средине. Интеракција загађивача са ћелијама имунског система може довести до поремећаја имунолошки-посредоване ткивне хомеостазе са штетним здравственим ефектима. Тешки метали спадају у најопасније загађиваче међу којима је кадмијум (Cd) један од најтоксичнијих, с обзиром на податке који показују да је токсичан при ниским концентрацијама и на студије које га повезују са имунолошки-посредованим поремећајима. Кадмијум најчешће доспева у организам оралним путем, уношењем контаминиране хране и воде, након чега се путем крви дистрибуира до свих органа где се депонује. На пацовском моделу продужене оралне примене Cd (дозе релевантне за срединску изложеност) показали смо да Cd остварује имуномодулаторне ефекте локално (у цревном тракту) али и у удаљеним ткивима плућа и коже. Оштећење ткива и инфламација (повећан оксидативни стрес и продукција проинфламаторних цитокина) запажени су у сва три органа. Поремећај имунске хомеостазе у цревима огледа се и у стимулацији ефекторског имунског одговора (пролиферација, IFN-γ, IL-17) у чворовима који дренирају ову регију. Инфламација у кожи и повећана оксидативна и проинфламаторна активност епидермних ћелија коинцидирају са повећаном реактивношћу овог ткива на контактни алерген. Утичући на активност имунског система орално примењен Cd може да мења његову функцију и последично осетљивост на развој болести, што указује да Cd може представљати озбиљну претњу по здравље људи.
AB  - Имунски систем је осетљива мета токсичног деловања загађивача из спољашње средине. Интеракција загађивача са ћелијама имунског система може довести до поремећаја имунолошки-посредоване ткивне хомеостазе са штетним здравственим ефектима. Тешки метали спадају у најопасније загађиваче међу којима је кадмијум (Cd) један од најтоксичнијих, с обзиром на податке који показују да је токсичан при ниским концентрацијама и на студије које га повезују са имунолошки-посредованим поремећајима. Кадмијум најчешће доспева у организам оралним путем, уношењем контаминиране хране и воде, након чега се путем крви дистрибуира до свих органа где се депонује. На пацовском моделу продужене оралне примене Cd (дозе релевантне за срединску изложеност) показали смо да Cd остварује имуномодулаторне ефекте локално (у цревном тракту) али и у удаљеним ткивима плућа и коже. Оштећење ткива и инфламација (повећан оксидативни стрес и продукција проинфламаторних цитокина) запажени су у сва три органа. Поремећај имунске хомеостазе у цревима огледа се и у стимулацији ефекторског имунског одговора (пролиферација, IFN-γ, IL-17) у чворовима који дренирају ову регију. Инфламација у кожи и повећана оксидативна и проинфламаторна активност епидермних ћелија коинцидирају са повећаном реактивношћу овог ткива на контактни алерген. Утичући на активност имунског система орално примењен Cd може да мења његову функцију и последично осетљивост на развој болести, што указује да Cd може представљати озбиљну претњу по здравље људи.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Imunomodulatorni efekti zagađivača iz spoljašnje sredine:primer teškog metala kadmijuma
T1  - Имуномодулаторни ефекти загађивача из спољашње средине:пример тешког метала кадмијума
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5141
ER  - 

@conference{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Tucović, Dina and Kulaš, Jelena and Ninkov, Marina and Popović, Dušanka and Malešević, Anastasija and Kataranovski, Milena",
year = "2022",
abstract = "Имунски систем је осетљива мета токсичног деловања загађивача из спољашње средине. Интеракција загађивача са ћелијама имунског система може довести до поремећаја имунолошки-посредоване ткивне хомеостазе са штетним здравственим ефектима. Тешки метали спадају у најопасније загађиваче међу којима је кадмијум (Cd) један од најтоксичнијих, с обзиром на податке који показују да је токсичан при ниским концентрацијама и на студије које га повезују са имунолошки-посредованим поремећајима. Кадмијум најчешће доспева у организам оралним путем, уношењем контаминиране хране и воде, након чега се путем крви дистрибуира до свих органа где се депонује. На пацовском моделу продужене оралне примене Cd (дозе релевантне за срединску изложеност) показали смо да Cd остварује имуномодулаторне ефекте локално (у цревном тракту) али и у удаљеним ткивима плућа и коже. Оштећење ткива и инфламација (повећан оксидативни стрес и продукција проинфламаторних цитокина) запажени су у сва три органа. Поремећај имунске хомеостазе у цревима огледа се и у стимулацији ефекторског имунског одговора (пролиферација, IFN-γ, IL-17) у чворовима који дренирају ову регију. Инфламација у кожи и повећана оксидативна и проинфламаторна активност епидермних ћелија коинцидирају са повећаном реактивношћу овог ткива на контактни алерген. Утичући на активност имунског система орално примењен Cd може да мења његову функцију и последично осетљивост на развој болести, што указује да Cd може представљати озбиљну претњу по здравље људи., Имунски систем је осетљива мета токсичног деловања загађивача из спољашње средине. Интеракција загађивача са ћелијама имунског система може довести до поремећаја имунолошки-посредоване ткивне хомеостазе са штетним здравственим ефектима. Тешки метали спадају у најопасније загађиваче међу којима је кадмијум (Cd) један од најтоксичнијих, с обзиром на податке који показују да је токсичан при ниским концентрацијама и на студије које га повезују са имунолошки-посредованим поремећајима. Кадмијум најчешће доспева у организам оралним путем, уношењем контаминиране хране и воде, након чега се путем крви дистрибуира до свих органа где се депонује. На пацовском моделу продужене оралне примене Cd (дозе релевантне за срединску изложеност) показали смо да Cd остварује имуномодулаторне ефекте локално (у цревном тракту) али и у удаљеним ткивима плућа и коже. Оштећење ткива и инфламација (повећан оксидативни стрес и продукција проинфламаторних цитокина) запажени су у сва три органа. Поремећај имунске хомеостазе у цревима огледа се и у стимулацији ефекторског имунског одговора (пролиферација, IFN-γ, IL-17) у чворовима који дренирају ову регију. Инфламација у кожи и повећана оксидативна и проинфламаторна активност епидермних ћелија коинцидирају са повећаном реактивношћу овог ткива на контактни алерген. Утичући на активност имунског система орално примењен Cd може да мења његову функцију и последично осетљивост на развој болести, што указује да Cd може представљати озбиљну претњу по здравље људи.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Imunomodulatorni efekti zagađivača iz spoljašnje sredine:primer teškog metala kadmijuma, Имуномодулаторни ефекти загађивача из спољашње средине:пример тешког метала кадмијума",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5141"
}

Popov Aleksandrov, A., Mirkov, I., Tucović, D., Kulaš, J., Ninkov, M., Popović, D., Malešević, A.,& Kataranovski, M.. (2022). Imunomodulatorni efekti zagađivača iz spoljašnje sredine:primer teškog metala kadmijuma. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 114.
https://hdl.handle.net/21.15107/rcub_ibiss_5141

Popov Aleksandrov A, Mirkov I, Tucović D, Kulaš J, Ninkov M, Popović D, Malešević A, Kataranovski M. Imunomodulatorni efekti zagađivača iz spoljašnje sredine:primer teškog metala kadmijuma. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:114.
https://hdl.handle.net/21.15107/rcub_ibiss_5141 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Tucović, Dina, Kulaš, Jelena, Ninkov, Marina, Popović, Dušanka, Malešević, Anastasija, Kataranovski, Milena, "Imunomodulatorni efekti zagađivača iz spoljašnje sredine:primer teškog metala kadmijuma" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):114,
https://hdl.handle.net/21.15107/rcub_ibiss_5141 .

TY  - CONF
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Popović, Dušanka
AU  - Malešević, Anastasija
AU  - Kataranovski, Milena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5142
AB  - In addition to physical and chemical insults originating from nature (heavy metals, radiation, electromagnetic field), human beings are exposed to various agents as a result of progress in industry and technology (noise, pesticides, pharmaceuticals, personal care products, food additives, etc.) whose number continuously increases. Some of these agents may have adverse effects on the environment and human health and therefore every new product must be examined for its toxic potential and health risk. The immune system is an important homeostatic system that is included in response to various pathogens, but in other processes as well including wound healing and elimination of damaged cells and tumors. Due to its involvement in various physiological processes, alternation in immune system activity, as a consequence of exposure to xenobiotics, may cause an increase in the incidence of infections, tumors, allergies, and autoimmune diseases. Various tests that exist for the evaluation of immunotoxicity are grouped in two separate sets, tier one (experiments directed to measure the effect of xenobiotics on general immune parameters i.e. basic morphological and functional tests) and tier two(experiments aimed to more specifically define the cellular and biochemical mechanisms of toxicity). Evaluation of potential adverse effects of xenobiotics on the immune system is now incorporated into standard hazard identification and has improved human health risk assessment.
AB  - Pored fizičkih i hemijskih agenasa poreklom iz prirode (teški metali, zračenje, elektromagnetno polje), ljudska bića su izložena raznim agensima kao rezultat napretka industrije i tehnologije (buka, pesticidi, farmaceutski proizvodi, proizvodi za ličnu negu, aditivi u hrani, itd.) čiji se broj kontinuirano povećava. Neki od ovih agenasa mogu imati štetne efekte na životnu sredinu i zdravlje ljudi i stoga za svaki novi proizvod mora biti ispitan njegov toksični potencijal i rizik po zdravlje. Imunski sistem je važan homeostatski sistem koji je uključen u odgovor na različite patogene, ali i u druge procese kao što su zarastanje rana, eliminaciju oštećenih ćelija i tumora. Zbog uključenosti u različite fiziološke procese, promenjena aktivnost imunskog sistema, kao posledica izloženosti ksenobioticima, može dovesti do povećanja učestalosti infekcija, tumora, alergija i autoimunskih bolesti. Postoje različiti testovi za procenu imunotoksičnosti koji su grupisani u dva odvojena seta, prvi nivo (eksperimenti usmereni na merenje uticaja ksenobiotika na opšte imunološke parametre, tj. osnovni morfološki i funkcionalni testovi) i nivo dva (eksperimenti koji imaju za cilj da preciznije definišu ćelijske i biohemijske mehanizme toksičnosti). Procena potencijalnih štetnih efekata ksenobiotika na imunski sistem je sada uključena u standardnu identifikaciju opasnosti i poboljšala je procenu rizika koju ksenobiotici mogu imati po zdravlje ljudi.
PB  - Belgrade: Serbian Society of Corrosion and Materials Protection
C3  - Proceedings: Meeting point of the science and practice in the fields of corrosion, materials and environmental protection: XXIII YuCorr International Conference; 2022 May 16-19; Divčibare, Serbia
T1  - Immune System as a Target of Xenobiotics Toxicity
T1  - Imunski sistem kao meta toksičnog delovanja ksenobiotika
SP  - 7
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5142
ER  - 

@conference{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Popović, Dušanka and Malešević, Anastasija and Kataranovski, Milena",
year = "2022",
abstract = "In addition to physical and chemical insults originating from nature (heavy metals, radiation, electromagnetic field), human beings are exposed to various agents as a result of progress in industry and technology (noise, pesticides, pharmaceuticals, personal care products, food additives, etc.) whose number continuously increases. Some of these agents may have adverse effects on the environment and human health and therefore every new product must be examined for its toxic potential and health risk. The immune system is an important homeostatic system that is included in response to various pathogens, but in other processes as well including wound healing and elimination of damaged cells and tumors. Due to its involvement in various physiological processes, alternation in immune system activity, as a consequence of exposure to xenobiotics, may cause an increase in the incidence of infections, tumors, allergies, and autoimmune diseases. Various tests that exist for the evaluation of immunotoxicity are grouped in two separate sets, tier one (experiments directed to measure the effect of xenobiotics on general immune parameters i.e. basic morphological and functional tests) and tier two(experiments aimed to more specifically define the cellular and biochemical mechanisms of toxicity). Evaluation of potential adverse effects of xenobiotics on the immune system is now incorporated into standard hazard identification and has improved human health risk assessment., Pored fizičkih i hemijskih agenasa poreklom iz prirode (teški metali, zračenje, elektromagnetno polje), ljudska bića su izložena raznim agensima kao rezultat napretka industrije i tehnologije (buka, pesticidi, farmaceutski proizvodi, proizvodi za ličnu negu, aditivi u hrani, itd.) čiji se broj kontinuirano povećava. Neki od ovih agenasa mogu imati štetne efekte na životnu sredinu i zdravlje ljudi i stoga za svaki novi proizvod mora biti ispitan njegov toksični potencijal i rizik po zdravlje. Imunski sistem je važan homeostatski sistem koji je uključen u odgovor na različite patogene, ali i u druge procese kao što su zarastanje rana, eliminaciju oštećenih ćelija i tumora. Zbog uključenosti u različite fiziološke procese, promenjena aktivnost imunskog sistema, kao posledica izloženosti ksenobioticima, može dovesti do povećanja učestalosti infekcija, tumora, alergija i autoimunskih bolesti. Postoje različiti testovi za procenu imunotoksičnosti koji su grupisani u dva odvojena seta, prvi nivo (eksperimenti usmereni na merenje uticaja ksenobiotika na opšte imunološke parametre, tj. osnovni morfološki i funkcionalni testovi) i nivo dva (eksperimenti koji imaju za cilj da preciznije definišu ćelijske i biohemijske mehanizme toksičnosti). Procena potencijalnih štetnih efekata ksenobiotika na imunski sistem je sada uključena u standardnu identifikaciju opasnosti i poboljšala je procenu rizika koju ksenobiotici mogu imati po zdravlje ljudi.",
publisher = "Belgrade: Serbian Society of Corrosion and Materials Protection",
journal = "Proceedings: Meeting point of the science and practice in the fields of corrosion, materials and environmental protection: XXIII YuCorr International Conference; 2022 May 16-19; Divčibare, Serbia",
title = "Immune System as a Target of Xenobiotics Toxicity, Imunski sistem kao meta toksičnog delovanja ksenobiotika",
pages = "7-13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5142"
}

Mirkov, I., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Popović, D., Malešević, A.,& Kataranovski, M.. (2022). Immune System as a Target of Xenobiotics Toxicity. in Proceedings: Meeting point of the science and practice in the fields of corrosion, materials and environmental protection: XXIII YuCorr International Conference; 2022 May 16-19; Divčibare, Serbia
Belgrade: Serbian Society of Corrosion and Materials Protection., 7-13.
https://hdl.handle.net/21.15107/rcub_ibiss_5142

Mirkov I, Popov Aleksandrov A, Tucović D, Kulaš J, Popović D, Malešević A, Kataranovski M. Immune System as a Target of Xenobiotics Toxicity. in Proceedings: Meeting point of the science and practice in the fields of corrosion, materials and environmental protection: XXIII YuCorr International Conference; 2022 May 16-19; Divčibare, Serbia. 2022;:7-13.
https://hdl.handle.net/21.15107/rcub_ibiss_5142 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Popović, Dušanka, Malešević, Anastasija, Kataranovski, Milena, "Immune System as a Target of Xenobiotics Toxicity" in Proceedings: Meeting point of the science and practice in the fields of corrosion, materials and environmental protection: XXIII YuCorr International Conference; 2022 May 16-19; Divčibare, Serbia (2022):7-13,
https://hdl.handle.net/21.15107/rcub_ibiss_5142 .

TY  - CONF
AU  - Tucović, Dina
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Despotović, Marta
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4882
AB  - Cadmium (Cd) in food and drinking water presents a health risk to the general population. We have shown previously that orally‐acquired Cd affects basal immune homeostasis in the skin. In this study, we examined the effect of 30‐days oral exposure of inflammatory disease‐prone Dark Agouti (DA) rats to two environmentally relevant Cd doses (5 and 50 ppm) on contact hypersensitivity reaction (CHS) induced by topical 0,4% dinitrochlorobenzene (DNCB). Both Cd doses increased proinflammatory epidermal cell response (IL‐1, TNF and IL‐6 production) to DNCB sensitization, as well as epidermal cells’ potential to stimulate naïve lymphocytes ex vivo (increased IFN‐γ and IL‐17 production in co‐cultures). The proinflammatory milieu of epidermal cells induced by sensitization was accompanied by increased hapten‐specific production of IFN‐γ (at a lower Cd dose) and IL‐17 (at both Cd doses) by draining lymph node (DLN) cells, compared to Cd non‐treated animals. During the challenge phase of CHS, oral Cd increased ear swelling response and skin inflammation (edema, mononuclear and neutrophil cell infiltration) at both Cd doses, what correlated with increased innate (TNF) and hapten‐specific effector (IFN‐γ, IL‐17) cytokine response by ear cells. Even in Albino Oxford (AO) rats generally less prone to inflammation, oral Cd increased the proinflammatory response of epidermal cells following sensitization, however, DLN cell responses were absent. Ear swelling response to hapten challenge was observed in AO individuals which acquired a higher Cd dose. Presented data imply the potential of food‐ and water‐borne Cd to be risk factors for skin disease development and/or its exacerbation.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Oral cadmium increases contact hypersensitivity reaction in rats
DO  - 10.1002/eji.202170200
SP  - 348
ER  - 

@conference{
author = "Tucović, Dina and Mirkov, Ivana and Kulaš, Jelena and Popović, Dušanka and Zolotarevski, Lidija and Despotović, Marta and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2021",
abstract = "Cadmium (Cd) in food and drinking water presents a health risk to the general population. We have shown previously that orally‐acquired Cd affects basal immune homeostasis in the skin. In this study, we examined the effect of 30‐days oral exposure of inflammatory disease‐prone Dark Agouti (DA) rats to two environmentally relevant Cd doses (5 and 50 ppm) on contact hypersensitivity reaction (CHS) induced by topical 0,4% dinitrochlorobenzene (DNCB). Both Cd doses increased proinflammatory epidermal cell response (IL‐1, TNF and IL‐6 production) to DNCB sensitization, as well as epidermal cells’ potential to stimulate naïve lymphocytes ex vivo (increased IFN‐γ and IL‐17 production in co‐cultures). The proinflammatory milieu of epidermal cells induced by sensitization was accompanied by increased hapten‐specific production of IFN‐γ (at a lower Cd dose) and IL‐17 (at both Cd doses) by draining lymph node (DLN) cells, compared to Cd non‐treated animals. During the challenge phase of CHS, oral Cd increased ear swelling response and skin inflammation (edema, mononuclear and neutrophil cell infiltration) at both Cd doses, what correlated with increased innate (TNF) and hapten‐specific effector (IFN‐γ, IL‐17) cytokine response by ear cells. Even in Albino Oxford (AO) rats generally less prone to inflammation, oral Cd increased the proinflammatory response of epidermal cells following sensitization, however, DLN cell responses were absent. Ear swelling response to hapten challenge was observed in AO individuals which acquired a higher Cd dose. Presented data imply the potential of food‐ and water‐borne Cd to be risk factors for skin disease development and/or its exacerbation.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Oral cadmium increases contact hypersensitivity reaction in rats",
doi = "10.1002/eji.202170200",
pages = "348"
}

Tucović, D., Mirkov, I., Kulaš, J., Popović, D., Zolotarevski, L., Despotović, M., Kataranovski, M.,& Popov Aleksandrov, A.. (2021). Oral cadmium increases contact hypersensitivity reaction in rats. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 348.
https://doi.org/10.1002/eji.202170200

Tucović D, Mirkov I, Kulaš J, Popović D, Zolotarevski L, Despotović M, Kataranovski M, Popov Aleksandrov A. Oral cadmium increases contact hypersensitivity reaction in rats. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;:348.
doi:10.1002/eji.202170200 .

Tucović, Dina, Mirkov, Ivana, Kulaš, Jelena, Popović, Dušanka, Zolotarevski, Lidija, Despotović, Marta, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Oral cadmium increases contact hypersensitivity reaction in rats" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting (2021):348,
https://doi.org/10.1002/eji.202170200 . .

TY  - JOUR
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Ukropina, Mirela
AU  - Cakić Milošević, Maja
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0300483X20302730
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4033
AB  - Cadmium (Cd) is one of the most toxic environmental heavy metals to which the general population is exposed mainly via the oral route. Owing to its immunomodulatory potential, orally acquired Cd affects antimicrobial immune defense in several organs, including the lungs. While there are data concerning Cd and viral and bacterial pulmonary infections, effects on fungal infections are not studied yet. In the present study, the effect of the Cd (5 mg/L for 30 days, in drinking water, the average daily Cd intake 0.641 ± 0.089 mg/kg) on the immune response of rats to pulmonary A. fumigatus infection was examined. Data obtained showed that orally acquired cadmium does not affect the elimination of the fungus in immunocompetent rats owing to the preservation of some aspects of innate immune responses (lung leukocyte infiltration and NBT reduction) and an increase in other (increased numbers of mucus-producing goblet cells, MPO release). Cd does not affect an IFN-γ response in lung leukocytes during the infection (despite suppression of cytokine production in cells of lung-draining lymph nodes), while it stimulates IL-17 and suppresses IL-10 response to the fungus. As a result, the elimination of the fungus occurs in a milieu with the prevailing proinflammatory response in Cd-exposed animals that preserved fungal elimination from the lungs, though with more intense injury to the lung tissue. Therefore, the proinflammatory microenvironment in the lungs created by Cd that sustains inflammatory/immune response to the fungus to which humans are exposed for a lifetime, raises a concern of orally acquired Cd as a risk factor for the development of chronic low-grade pulmonary inflammation.
PB  - Elsevier Ireland Ltd
T2  - Toxicology
T2  - Toxicology
T1  - Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?
VL  - 447
DO  - 10.1016/j.tox.2020.152634
SP  - 152634
ER  - 

@article{
author = "Kulaš, Jelena and Tucović, Dina and Zeljković, Milica and Popović, Dušanka and Popov Aleksandrov, Aleksandra and Ukropina, Mirela and Cakić Milošević, Maja and Glamočlija, Jasmina and Kataranovski, Milena and Mirkov, Ivana",
year = "2021",
abstract = "Cadmium (Cd) is one of the most toxic environmental heavy metals to which the general population is exposed mainly via the oral route. Owing to its immunomodulatory potential, orally acquired Cd affects antimicrobial immune defense in several organs, including the lungs. While there are data concerning Cd and viral and bacterial pulmonary infections, effects on fungal infections are not studied yet. In the present study, the effect of the Cd (5 mg/L for 30 days, in drinking water, the average daily Cd intake 0.641 ± 0.089 mg/kg) on the immune response of rats to pulmonary A. fumigatus infection was examined. Data obtained showed that orally acquired cadmium does not affect the elimination of the fungus in immunocompetent rats owing to the preservation of some aspects of innate immune responses (lung leukocyte infiltration and NBT reduction) and an increase in other (increased numbers of mucus-producing goblet cells, MPO release). Cd does not affect an IFN-γ response in lung leukocytes during the infection (despite suppression of cytokine production in cells of lung-draining lymph nodes), while it stimulates IL-17 and suppresses IL-10 response to the fungus. As a result, the elimination of the fungus occurs in a milieu with the prevailing proinflammatory response in Cd-exposed animals that preserved fungal elimination from the lungs, though with more intense injury to the lung tissue. Therefore, the proinflammatory microenvironment in the lungs created by Cd that sustains inflammatory/immune response to the fungus to which humans are exposed for a lifetime, raises a concern of orally acquired Cd as a risk factor for the development of chronic low-grade pulmonary inflammation.",
publisher = "Elsevier Ireland Ltd",
journal = "Toxicology, Toxicology",
title = "Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?",
volume = "447",
doi = "10.1016/j.tox.2020.152634",
pages = "152634"
}

Kulaš, J., Tucović, D., Zeljković, M., Popović, D., Popov Aleksandrov, A., Ukropina, M., Cakić Milošević, M., Glamočlija, J., Kataranovski, M.,& Mirkov, I.. (2021). Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?. in Toxicology
Elsevier Ireland Ltd., 447, 152634.
https://doi.org/10.1016/j.tox.2020.152634

Kulaš J, Tucović D, Zeljković M, Popović D, Popov Aleksandrov A, Ukropina M, Cakić Milošević M, Glamočlija J, Kataranovski M, Mirkov I. Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?. in Toxicology. 2021;447:152634.
doi:10.1016/j.tox.2020.152634 .

Kulaš, Jelena, Tucović, Dina, Zeljković, Milica, Popović, Dušanka, Popov Aleksandrov, Aleksandra, Ukropina, Mirela, Cakić Milošević, Maja, Glamočlija, Jasmina, Kataranovski, Milena, Mirkov, Ivana, "Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?" in Toxicology, 447 (2021):152634,
https://doi.org/10.1016/j.tox.2020.152634 . .

TY  - JOUR
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2021
UR  - http://www.besjournal.com/en/article/doi/10.3967/bes2021.025
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4191
AB  - Objective To investigate involvement of the aryl hydrocarbon receptor (AhR) in the immunomodulatory effects of cadmium (Cd). Methods The effect of Cd on AhR activation ( CYP1A1 and CYP1B1 mRNA expression) was examined in lung leukocytes of Cd-exposed rats (5 and 50 mg/L, 30 d orally) and by in vitro leukocyte exposure. The involvement of AhR signaling in the effects of Cd on the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) lung leukocyte response was investigated in vitro using the receptor antagonist CH-223191. Results Cd increased CYP1B1 ( in vivo and in vitro) and CYP1A1 ( in vitro) mRNA, indicating AhR involvement in the action of Cd. In response to Cd, lung leukocytes increased IL-6 and decreased TNF at the gene expression and protein levels, but decreased IL-1β production due to reduced NLRP3. The AhR antagonist CH-223191 abrogated the observed effects of Cd on the cytokine response. The absence of AhR reactivity and cytokine response to Cd of leukocytes from the lungs of a rat strain that is less sensitive to Cd toxicity coincided with a high AhR repressor mRNA level. Conclusion AhR signaling is involved in the lung leukocyte proinflammatory cytokine response to Cd. The relevance of the AhR to the cytokine response to Cd provides new insight into the mechanisms of Cd immunotoxicity.
PB  - Elsevier Ltd
T2  - Biomedical and Environmental Sciences
T1  - Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.
IS  - 3
VL  - 34
DO  - 10.3967/bes2021.025
SP  - 192
EP  - 202
ER  - 

@article{
author = "Kulaš, Jelena and Tucović, Dina and Zeljković, Milica and Popović, Dušanka and Popov Aleksandrov, Aleksandra and Kataranovski, Milena and Mirkov, Ivana",
year = "2021",
abstract = "Objective To investigate involvement of the aryl hydrocarbon receptor (AhR) in the immunomodulatory effects of cadmium (Cd). Methods The effect of Cd on AhR activation ( CYP1A1 and CYP1B1 mRNA expression) was examined in lung leukocytes of Cd-exposed rats (5 and 50 mg/L, 30 d orally) and by in vitro leukocyte exposure. The involvement of AhR signaling in the effects of Cd on the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) lung leukocyte response was investigated in vitro using the receptor antagonist CH-223191. Results Cd increased CYP1B1 ( in vivo and in vitro) and CYP1A1 ( in vitro) mRNA, indicating AhR involvement in the action of Cd. In response to Cd, lung leukocytes increased IL-6 and decreased TNF at the gene expression and protein levels, but decreased IL-1β production due to reduced NLRP3. The AhR antagonist CH-223191 abrogated the observed effects of Cd on the cytokine response. The absence of AhR reactivity and cytokine response to Cd of leukocytes from the lungs of a rat strain that is less sensitive to Cd toxicity coincided with a high AhR repressor mRNA level. Conclusion AhR signaling is involved in the lung leukocyte proinflammatory cytokine response to Cd. The relevance of the AhR to the cytokine response to Cd provides new insight into the mechanisms of Cd immunotoxicity.",
publisher = "Elsevier Ltd",
journal = "Biomedical and Environmental Sciences",
title = "Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.",
number = "3",
volume = "34",
doi = "10.3967/bes2021.025",
pages = "192-202"
}

Kulaš, J., Tucović, D., Zeljković, M., Popović, D., Popov Aleksandrov, A., Kataranovski, M.,& Mirkov, I.. (2021). Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.. in Biomedical and Environmental Sciences
Elsevier Ltd., 34(3), 192-202.
https://doi.org/10.3967/bes2021.025

Kulaš J, Tucović D, Zeljković M, Popović D, Popov Aleksandrov A, Kataranovski M, Mirkov I. Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.. in Biomedical and Environmental Sciences. 2021;34(3):192-202.
doi:10.3967/bes2021.025 .

Kulaš, Jelena, Tucović, Dina, Zeljković, Milica, Popović, Dušanka, Popov Aleksandrov, Aleksandra, Kataranovski, Milena, Mirkov, Ivana, "Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium." in Biomedical and Environmental Sciences, 34, no. 3 (2021):192-202,
https://doi.org/10.3967/bes2021.025 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Ninkov, Marina
AU  - Janković, Srđa
AU  - Kataranovski, Milena
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0165247821001644
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4664
AB  - Cadmium (Cd) represents a unique hazard because of the long biological half-life in humans (20-30 years). This metal accumulates in organs causing a continuum of responses, with organ disease/failure as extreme outcome. Some of the cellular and molecular alterations in target tissues can be related to immune-modulating potential of Cd. This metal may cause adverse responses in which components of the immune system function as both mediators and effectors of Cd tissue toxicity, which, in combination with Cd-induced alterations in homeostatic reparative activities may contribute to tissue dysfunction. In this work, current knowledge concerning inflammatory/autoimmune disease manifestations found to be related with cadmium exposure are summarized. Along with epidemiological evidence, animal and in vitro data are presented, with focus on cellular and molecular immune mechanisms potentially relevant for the disease susceptibility, disease promotion, or facilitating development of pre-existing pathologies.
PB  - Elsevier B.V.
T2  - Immunology Letters
T1  - Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.
VL  - 240
DO  - 10.1016/j.imlet.2021.10.003
SP  - 106
EP  - 122
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Tucović, Dina and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Ninkov, Marina and Janković, Srđa and Kataranovski, Milena",
year = "2021",
abstract = "Cadmium (Cd) represents a unique hazard because of the long biological half-life in humans (20-30 years). This metal accumulates in organs causing a continuum of responses, with organ disease/failure as extreme outcome. Some of the cellular and molecular alterations in target tissues can be related to immune-modulating potential of Cd. This metal may cause adverse responses in which components of the immune system function as both mediators and effectors of Cd tissue toxicity, which, in combination with Cd-induced alterations in homeostatic reparative activities may contribute to tissue dysfunction. In this work, current knowledge concerning inflammatory/autoimmune disease manifestations found to be related with cadmium exposure are summarized. Along with epidemiological evidence, animal and in vitro data are presented, with focus on cellular and molecular immune mechanisms potentially relevant for the disease susceptibility, disease promotion, or facilitating development of pre-existing pathologies.",
publisher = "Elsevier B.V.",
journal = "Immunology Letters",
title = "Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.",
volume = "240",
doi = "10.1016/j.imlet.2021.10.003",
pages = "106-122"
}

Popov Aleksandrov, A., Mirkov, I., Tucović, D., Kulaš, J., Zeljković, M., Popović, D., Ninkov, M., Janković, S.,& Kataranovski, M.. (2021). Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.. in Immunology Letters
Elsevier B.V.., 240, 106-122.
https://doi.org/10.1016/j.imlet.2021.10.003

Popov Aleksandrov A, Mirkov I, Tucović D, Kulaš J, Zeljković M, Popović D, Ninkov M, Janković S, Kataranovski M. Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.. in Immunology Letters. 2021;240:106-122.
doi:10.1016/j.imlet.2021.10.003 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Tucović, Dina, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Ninkov, Marina, Janković, Srđa, Kataranovski, Milena, "Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example." in Immunology Letters, 240 (2021):106-122,
https://doi.org/10.1016/j.imlet.2021.10.003 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Kataranovski, Milena
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33508420
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4150
AB  - Cadmium (Cd) has been listed as one of the most toxic substances affecting numerous tissues/organs, including the immune system. Due to variations in studies examining Cd effects on the immune system (exposure regime, experimental systems, immune endpoint measured), data on Cd immunotoxicity in humans and experimental animals are inconsistent. However, it is clear that Cd can affect cells of the immune system and can modulate some immune responses. Due to the complex nature of the immune system and its activities which are determined by multiple interactions, the underlying mechanisms involved in the immunotoxicity of this metal are still vague. Here, the current knowledge regarding the interaction of Cd with cells of the immune system, which may affect immune responses as well as potential mechanisms of consequent biological effects of such activities, is reviewed. Tissue injury caused by Cd-induced effects on innate cell activities depicts components of the immune system as mediators/effectors of Cd tissue toxicity. Cd-induced immune alterations, which may compromise host defense against pathogenic microorganisms and homeostatic reparative activities, stress this metal as an important health hazard.
PB  - Elsevier BV
T2  - Food and Chemical Toxicology
T1  - Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.
VL  - 149
DO  - 10.1016/j.fct.2021.112026
SP  - 112026
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Tucović, Dina and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Kataranovski, Milena",
year = "2021",
abstract = "Cadmium (Cd) has been listed as one of the most toxic substances affecting numerous tissues/organs, including the immune system. Due to variations in studies examining Cd effects on the immune system (exposure regime, experimental systems, immune endpoint measured), data on Cd immunotoxicity in humans and experimental animals are inconsistent. However, it is clear that Cd can affect cells of the immune system and can modulate some immune responses. Due to the complex nature of the immune system and its activities which are determined by multiple interactions, the underlying mechanisms involved in the immunotoxicity of this metal are still vague. Here, the current knowledge regarding the interaction of Cd with cells of the immune system, which may affect immune responses as well as potential mechanisms of consequent biological effects of such activities, is reviewed. Tissue injury caused by Cd-induced effects on innate cell activities depicts components of the immune system as mediators/effectors of Cd tissue toxicity. Cd-induced immune alterations, which may compromise host defense against pathogenic microorganisms and homeostatic reparative activities, stress this metal as an important health hazard.",
publisher = "Elsevier BV",
journal = "Food and Chemical Toxicology",
title = "Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.",
volume = "149",
doi = "10.1016/j.fct.2021.112026",
pages = "112026"
}

Mirkov, I., Popov Aleksandrov, A., Ninkov, M., Tucović, D., Kulaš, J., Zeljković, M., Popović, D.,& Kataranovski, M.. (2021). Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.. in Food and Chemical Toxicology
Elsevier BV., 149, 112026.
https://doi.org/10.1016/j.fct.2021.112026

Mirkov I, Popov Aleksandrov A, Ninkov M, Tucović D, Kulaš J, Zeljković M, Popović D, Kataranovski M. Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.. in Food and Chemical Toxicology. 2021;149:112026.
doi:10.1016/j.fct.2021.112026 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Tucović, Dina, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Kataranovski, Milena, "Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity." in Food and Chemical Toxicology, 149 (2021):112026,
https://doi.org/10.1016/j.fct.2021.112026 . .

TY  - CONF
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Terzić-Vidojević, Amarela
AU  - Lukić, Jovana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Tolinački, Maja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4793
AB  - The gut microbiome is very important for hosts’ proper essential functions, as microbiota influence both near and distant organs and disruption at any level of this complex relation can be underlying cause for many diseases. Both intestinal bacterial population and their metabolic products are involved in immune system development, activity and maintenance of immune homeostasis. Previous findings revealed differences in immune reactivity in Albino Oxford (AO) and Dark Agouti (DA) rat strains in various diseases models (such as experimental autoimmune encephalomyelitis, rheumatoid arthritis, contact hypersensitivity reaction, pulmonary aspergillosis etc.), and in response to xenobiotics such as cadmium, warfarin, etc.
The aim of present study was to investigate microbial composition of gut (duodenum, jejunum, coecum and colon) and lungs of AO and DA rats using DGGE method. Although similar number of bacterial species were noted in both tissue and lumen of duodenum, bacterial composition differ between these two strains (solely 76.2% and 44.4% species were common in both strains tissue and lumen, respectively), while greater variability was noted in DA rats. Similar results were noted in jejunum. In contrast to duodenum and jejunum, higher number of bacterial species were detected in coecum (content) and colon (tissue and content) of AO rats. Around 50% of detected bacteria were present in both gut segments of both strains. Analysis of DGGE bands obtained from lung tissue revealed similar number of bacteria in both strains, but solely 54.5% were common. Further investigations will be directed to identification of bacterial species and try to connect observed differences in microbial composition with different immune reactivity in AO and DA rats.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4793
ER  - 

@conference{
author = "Popović, Dušanka and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Zeljković, Milica and Terzić-Vidojević, Amarela and Lukić, Jovana and Golić, Nataša and Mirkov, Ivana and Tolinački, Maja",
year = "2019",
abstract = "The gut microbiome is very important for hosts’ proper essential functions, as microbiota influence both near and distant organs and disruption at any level of this complex relation can be underlying cause for many diseases. Both intestinal bacterial population and their metabolic products are involved in immune system development, activity and maintenance of immune homeostasis. Previous findings revealed differences in immune reactivity in Albino Oxford (AO) and Dark Agouti (DA) rat strains in various diseases models (such as experimental autoimmune encephalomyelitis, rheumatoid arthritis, contact hypersensitivity reaction, pulmonary aspergillosis etc.), and in response to xenobiotics such as cadmium, warfarin, etc.
The aim of present study was to investigate microbial composition of gut (duodenum, jejunum, coecum and colon) and lungs of AO and DA rats using DGGE method. Although similar number of bacterial species were noted in both tissue and lumen of duodenum, bacterial composition differ between these two strains (solely 76.2% and 44.4% species were common in both strains tissue and lumen, respectively), while greater variability was noted in DA rats. Similar results were noted in jejunum. In contrast to duodenum and jejunum, higher number of bacterial species were detected in coecum (content) and colon (tissue and content) of AO rats. Around 50% of detected bacteria were present in both gut segments of both strains. Analysis of DGGE bands obtained from lung tissue revealed similar number of bacteria in both strains, but solely 54.5% were common. Further investigations will be directed to identification of bacterial species and try to connect observed differences in microbial composition with different immune reactivity in AO and DA rats.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4793"
}

Popović, D., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Zeljković, M., Terzić-Vidojević, A., Lukić, J., Golić, N., Mirkov, I.,& Tolinački, M.. (2019). Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_4793

Popović D, Popov Aleksandrov A, Tucović D, Kulaš J, Zeljković M, Terzić-Vidojević A, Lukić J, Golić N, Mirkov I, Tolinački M. Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_4793 .

Popović, Dušanka, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Zeljković, Milica, Terzić-Vidojević, Amarela, Lukić, Jovana, Golić, Nataša, Mirkov, Ivana, Tolinački, Maja, "Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):28,
https://hdl.handle.net/21.15107/rcub_ibiss_4793 .

TY  - CONF
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4794
AB  - Cadmium (Cd) is a heavy metal widely spread in the environment and significant water and food contaminant. This metal exerts toxic effects in various tissues thus representing great threat to human health, and our previous study showed that oral consumption of Cd (in water for 30 days) increased the metal deposition and exerted immunomodulatory effects in lung leukocytes. Although the most studied mechanisms of Cd toxicity include oxidative stress and inflammation, recent studies have indicated that this metal can activate aryl hydrocarbon receptor (AHR) and exert effects on AHR-regulated genes (i.e. CYPs). AHR represents a link between environmental toxicants and immune response as high receptor expression is noted in immune cells and barrier tissues, thus the aim of presented study was to investigate if activation of AhR by Cd is associated with metals’ immunomodulatory effects. Treatment of lung leukocytes with Cd in vitro (non-toxic doses) caused an increase in mRNA levels for AHR, CYP1B1 and CYP1A1, but co-treatment with metal and AHR antagonist CH223191 indicated that higher Cd doses (5 and 10 μM) can activate CYPs directly while a lower dose (1 μM) exerted effects on CYPs expression through activation of AHR. Low Cd dose induced increased production of IL-6 and decreased TNF and IL-1β by lung leukocytes, compared to controls. Gene expression data revealed unchanged mRNA for IL-6, decreased TNF, but increased IL-1β. Lower IL-1β protein level despite increased mRNA, was a consequence of decreased mRNA for NLRP3, a component of inflammasome that is involved in processing of pro-IL-1β in IL-1β. All noted effects were abolished in the presence of CH223191. Data obtained indicate that immunomodulatory effects of low Cd dose are mediated through AHR activation.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4794
ER  - 

@conference{
author = "Kulaš, Jelena and Tucović, Dina and Zeljković, Milica and Popović, Dušanka and Popov Aleksandrov, Aleksandra and Kataranovski, Milena and Mirkov, Ivana",
year = "2019",
abstract = "Cadmium (Cd) is a heavy metal widely spread in the environment and significant water and food contaminant. This metal exerts toxic effects in various tissues thus representing great threat to human health, and our previous study showed that oral consumption of Cd (in water for 30 days) increased the metal deposition and exerted immunomodulatory effects in lung leukocytes. Although the most studied mechanisms of Cd toxicity include oxidative stress and inflammation, recent studies have indicated that this metal can activate aryl hydrocarbon receptor (AHR) and exert effects on AHR-regulated genes (i.e. CYPs). AHR represents a link between environmental toxicants and immune response as high receptor expression is noted in immune cells and barrier tissues, thus the aim of presented study was to investigate if activation of AhR by Cd is associated with metals’ immunomodulatory effects. Treatment of lung leukocytes with Cd in vitro (non-toxic doses) caused an increase in mRNA levels for AHR, CYP1B1 and CYP1A1, but co-treatment with metal and AHR antagonist CH223191 indicated that higher Cd doses (5 and 10 μM) can activate CYPs directly while a lower dose (1 μM) exerted effects on CYPs expression through activation of AHR. Low Cd dose induced increased production of IL-6 and decreased TNF and IL-1β by lung leukocytes, compared to controls. Gene expression data revealed unchanged mRNA for IL-6, decreased TNF, but increased IL-1β. Lower IL-1β protein level despite increased mRNA, was a consequence of decreased mRNA for NLRP3, a component of inflammasome that is involved in processing of pro-IL-1β in IL-1β. All noted effects were abolished in the presence of CH223191. Data obtained indicate that immunomodulatory effects of low Cd dose are mediated through AHR activation.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4794"
}

Kulaš, J., Tucović, D., Zeljković, M., Popović, D., Popov Aleksandrov, A., Kataranovski, M.,& Mirkov, I.. (2019). Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_4794

Kulaš J, Tucović D, Zeljković M, Popović D, Popov Aleksandrov A, Kataranovski M, Mirkov I. Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_4794 .

Kulaš, Jelena, Tucović, Dina, Zeljković, Milica, Popović, Dušanka, Popov Aleksandrov, Aleksandra, Kataranovski, Milena, Mirkov, Ivana, "Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):80,
https://hdl.handle.net/21.15107/rcub_ibiss_4794 .

TY  - CONF
AU  - Tucović, Dina
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Đurđić, Slađana
AU  - Mutić, Jelena
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4795
AB  - Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly
acknowledged. Using a rat model of oral Cd exposure in drinking water we have shown
that skin is a target for this metal. Due to contribution of individual variability to the
intensity of cadmium toxicity, dermatotoxicity of two environmentally relevant Cd
doses (5 and 50 ppm) was examined in individuals of two rat strains, Albino Oxford
(AO) and Dark Agouti (DA), which differ in response to chemicals. A dose-dependent
accumulation of Cd in the skin/epidermal cells was noted in both strains, and although
there were no strain differences in the Cd accumulation, the degree of skin response to
the metal differed. Signs of skin damage were evident in both strains, but response to
injury was more pronounced in DA. Individuals of DA rats responded by an increase
in the levels of antioxidant defense enzymes in the skin already at lower dose, in
contrast to AO (which reacted to higher dose solely), implying higher sensitivity of DA
strain to Cd-induced toxicity. Epidermal cells from both strains developed stress
response, however increased GSH, and higher metallothionein/MT-1 and MT-2
mRNA, Nrf2 protein, apoptosis, Ahr and Cyp genes in AO, depicting this strain`s
ability to better defend against Cd insult. Epidermal cells` IL-1β, TNF and IL-6
response was induced by Cd in DA, while pro-inflammatory cytokine production was
unchanged in AO (though increased following stimulation with S. epidermidis), with
increased IL-10 as a possible underlying mechanism. T cells from non-exposed rats
produce more IFN-γ and IL-17 in co-culture with epidermal cell from Cd–exposed DA
rats what strengthens the view that this strain is more prone to metal’s dermatotoxicity.
These data give a new insight into repercussion of genetic variability to toxicity of
cadmium acquired by the skin via gut, bearing relevance for variations in the link
between dietary cadmium and inflammation-based skin pathologies.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Environmentally relevant exposure to cadmium and health risks: skin as target organ
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4795
ER  - 

@conference{
author = "Tucović, Dina and Mirkov, Ivana and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Zolotarevski, Lidija and Đurđić, Slađana and Mutić, Jelena and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2019",
abstract = "Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly
acknowledged. Using a rat model of oral Cd exposure in drinking water we have shown
that skin is a target for this metal. Due to contribution of individual variability to the
intensity of cadmium toxicity, dermatotoxicity of two environmentally relevant Cd
doses (5 and 50 ppm) was examined in individuals of two rat strains, Albino Oxford
(AO) and Dark Agouti (DA), which differ in response to chemicals. A dose-dependent
accumulation of Cd in the skin/epidermal cells was noted in both strains, and although
there were no strain differences in the Cd accumulation, the degree of skin response to
the metal differed. Signs of skin damage were evident in both strains, but response to
injury was more pronounced in DA. Individuals of DA rats responded by an increase
in the levels of antioxidant defense enzymes in the skin already at lower dose, in
contrast to AO (which reacted to higher dose solely), implying higher sensitivity of DA
strain to Cd-induced toxicity. Epidermal cells from both strains developed stress
response, however increased GSH, and higher metallothionein/MT-1 and MT-2
mRNA, Nrf2 protein, apoptosis, Ahr and Cyp genes in AO, depicting this strain`s
ability to better defend against Cd insult. Epidermal cells` IL-1β, TNF and IL-6
response was induced by Cd in DA, while pro-inflammatory cytokine production was
unchanged in AO (though increased following stimulation with S. epidermidis), with
increased IL-10 as a possible underlying mechanism. T cells from non-exposed rats
produce more IFN-γ and IL-17 in co-culture with epidermal cell from Cd–exposed DA
rats what strengthens the view that this strain is more prone to metal’s dermatotoxicity.
These data give a new insight into repercussion of genetic variability to toxicity of
cadmium acquired by the skin via gut, bearing relevance for variations in the link
between dietary cadmium and inflammation-based skin pathologies.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Environmentally relevant exposure to cadmium and health risks: skin as target organ",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4795"
}

Tucović, D., Mirkov, I., Kulaš, J., Zeljković, M., Popović, D., Zolotarevski, L., Đurđić, S., Mutić, J., Kataranovski, M.,& Popov Aleksandrov, A.. (2019). Environmentally relevant exposure to cadmium and health risks: skin as target organ. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_4795

Tucović D, Mirkov I, Kulaš J, Zeljković M, Popović D, Zolotarevski L, Đurđić S, Mutić J, Kataranovski M, Popov Aleksandrov A. Environmentally relevant exposure to cadmium and health risks: skin as target organ. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_4795 .

Tucović, Dina, Mirkov, Ivana, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Zolotarevski, Lidija, Đurđić, Slađana, Mutić, Jelena, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Environmentally relevant exposure to cadmium and health risks: skin as target organ" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):79,
https://hdl.handle.net/21.15107/rcub_ibiss_4795 .