TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6453
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
IS  - 1
VL  - 13
DO  - 10.3390/antibiotics13010041
SP  - 41
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
number = "1",
volume = "13",
doi = "10.3390/antibiotics13010041",
pages = "41"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
Basel: MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - https://www.mdpi.com/2309-608X/9/1/70
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9864295
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5461
AB  - Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity
IS  - 1
VL  - 9
DO  - 10.3390/jof9010070
SP  - 70
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Soković, Marina and Ćirić, Ana",
year = "2023",
abstract = "Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity",
number = "1",
volume = "9",
doi = "10.3390/jof9010070",
pages = "70"
}

Kostić, M., Ivanov, M., Fernandes, Â., Calhelha, R. C., Glamočlija, J., Barros, L., Soković, M.,& Ćirić, A.. (2023). A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi
Basel: MDPI., 9(1), 70.
https://doi.org/10.3390/jof9010070

Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Soković M, Ćirić A. A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi. 2023;9(1):70.
doi:10.3390/jof9010070 .

Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Soković, Marina, Ćirić, Ana, "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity" in Journal of Fungi, 9, no. 1 (2023):70,
https://doi.org/10.3390/jof9010070 . .

TY  - CHAP
AU  - Mitić, Vojislav
AU  - Lazović, Goran
AU  - Milošević, Dušan
AU  - Manojlović, Jelena
AU  - Ristanović, Elizabeta
AU  - Simeunović, Dragan
AU  - Tsay, Shwu-Chen
AU  - Milošević, Mimica
AU  - Soković, Marina
AU  - Vlahović, Branislav
PY  - 2023
UR  - https://link.springer.com/10.1007/978-3-031-17269-4_16
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5401
AB  - One of the main motivations for our research was to find a connection between the Brownian motion of microorganisms within fractal nature, with the idea of developing an appropriate procedure and method to control the microorganism’s motion direction and predict the position of the microorganism in time. In this paper, we have followed the results of the very rear microorganism’s motion sub-microstructures in the experimental microstructure analysisFractals already observed and published. All of these data have been good basis to describe the motion trajectory by time interval method and fractals. We successfully defined the diagrams in two and three-dimensions and we were able to establish the control of Brownian chaotic motion as a bridge between chaotic disorders to control disorder. This significant study opens a new possibility for future investigation and the new potential of total control of the microorganism motion. These perspectives and findings provide significant data for getting more information from these bio systems. They can also be applied, based on self-similarities and biomimetics, to particle physical systemMatterFractalss and matter, generally.
PB  - Springer
T2  - Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications
T1  - Brownian Motion Fractal Nature Frontiers Within the Matter
DO  - 10.1007/978-3-031-17269-4_16
SP  - 325
EP  - 343
ER  - 

@inbook{
editor = "Najman, Stevo, Mitić, Vojislav, Groth, Thomas, Barbeck, Mike, Chen, Po-Yu, Sun, Ziqi, Randjelović, Branislav",
author = "Mitić, Vojislav and Lazović, Goran and Milošević, Dušan and Manojlović, Jelena and Ristanović, Elizabeta and Simeunović, Dragan and Tsay, Shwu-Chen and Milošević, Mimica and Soković, Marina and Vlahović, Branislav",
year = "2023",
abstract = "One of the main motivations for our research was to find a connection between the Brownian motion of microorganisms within fractal nature, with the idea of developing an appropriate procedure and method to control the microorganism’s motion direction and predict the position of the microorganism in time. In this paper, we have followed the results of the very rear microorganism’s motion sub-microstructures in the experimental microstructure analysisFractals already observed and published. All of these data have been good basis to describe the motion trajectory by time interval method and fractals. We successfully defined the diagrams in two and three-dimensions and we were able to establish the control of Brownian chaotic motion as a bridge between chaotic disorders to control disorder. This significant study opens a new possibility for future investigation and the new potential of total control of the microorganism motion. These perspectives and findings provide significant data for getting more information from these bio systems. They can also be applied, based on self-similarities and biomimetics, to particle physical systemMatterFractalss and matter, generally.",
publisher = "Springer",
journal = "Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications",
booktitle = "Brownian Motion Fractal Nature Frontiers Within the Matter",
doi = "10.1007/978-3-031-17269-4_16",
pages = "325-343"
}

Najman, S., Mitić, V., Groth, T., Barbeck, M., Chen, P., Sun, Z., Randjelović, B., Mitić, V., Lazović, G., Milošević, D., Manojlović, J., Ristanović, E., Simeunović, D., Tsay, S., Milošević, M., Soković, M.,& Vlahović, B.. (2023). Brownian Motion Fractal Nature Frontiers Within the Matter. in Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications
Springer., 325-343.
https://doi.org/10.1007/978-3-031-17269-4_16

Najman S, Mitić V, Groth T, Barbeck M, Chen P, Sun Z, Randjelović B, Mitić V, Lazović G, Milošević D, Manojlović J, Ristanović E, Simeunović D, Tsay S, Milošević M, Soković M, Vlahović B. Brownian Motion Fractal Nature Frontiers Within the Matter. in Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications. 2023;:325-343.
doi:10.1007/978-3-031-17269-4_16 .

Najman, Stevo, Mitić, Vojislav, Groth, Thomas, Barbeck, Mike, Chen, Po-Yu, Sun, Ziqi, Randjelović, Branislav, Mitić, Vojislav, Lazović, Goran, Milošević, Dušan, Manojlović, Jelena, Ristanović, Elizabeta, Simeunović, Dragan, Tsay, Shwu-Chen, Milošević, Mimica, Soković, Marina, Vlahović, Branislav, "Brownian Motion Fractal Nature Frontiers Within the Matter" in Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications (2023):325-343,
https://doi.org/10.1007/978-3-031-17269-4_16 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Babotă, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Rocchetti, Gabriele
AU  - Zhang, Leilei
AU  - Lucini, Luigi
AU  - Crișan, Gianina
AU  - Mocan, Andrei
AU  - Vostinaru, Oliviu
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5502
AB  - Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.
PB  - Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
VL  - 14
DO  - 10.3389/fphar.2023.1115117
SP  - 1115117
ER  - 

@article{
author = "Babotă, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Stojković, Dejan and Soković, Marina and Rocchetti, Gabriele and Zhang, Leilei and Lucini, Luigi and Crișan, Gianina and Mocan, Andrei and Vostinaru, Oliviu",
year = "2023",
abstract = "Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania",
volume = "14",
doi = "10.3389/fphar.2023.1115117",
pages = "1115117"
}

Babotă, M., Frumuzachi, O., Nicolescu, A., Stojković, D., Soković, M., Rocchetti, G., Zhang, L., Lucini, L., Crișan, G., Mocan, A.,& Vostinaru, O.. (2023). Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology
Frontiers Media S.A.., 14, 1115117.
https://doi.org/10.3389/fphar.2023.1115117

Babotă M, Frumuzachi O, Nicolescu A, Stojković D, Soković M, Rocchetti G, Zhang L, Lucini L, Crișan G, Mocan A, Vostinaru O. Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology. 2023;14:1115117.
doi:10.3389/fphar.2023.1115117 .

Babotă, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Stojković, Dejan, Soković, Marina, Rocchetti, Gabriele, Zhang, Leilei, Lucini, Luigi, Crișan, Gianina, Mocan, Andrei, Vostinaru, Oliviu, "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania" in Frontiers in Pharmacology, 14 (2023):1115117,
https://doi.org/10.3389/fphar.2023.1115117 . .

TY  - JOUR
AU  - Merlani, Maia
AU  - Nadaraia, Nanuli
AU  - Amiranashvili, Lela
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Carević, Tamara
AU  - Soković, Marina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5659
AB  - Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.
PB  - Basel: MDPI
T2  - Molecules
T1  - Antimicrobial Activity of Some Steroidal Hydrazones
IS  - 3
VL  - 28
DO  - 10.3390/molecules28031167
SP  - 1167
ER  - 

@article{
author = "Merlani, Maia and Nadaraia, Nanuli and Amiranashvili, Lela and Petrou, Anthi and Geronikaki, Athina and Ćirić, Ana and Glamočlija, Jasmina and Carević, Tamara and Soković, Marina",
year = "2023",
abstract = "Twelve steroid based hydrazones were in silico evaluated using computer program PASS as antimicrobial agents. The experimental evaluation revealed that all compounds have low to moderate antibacterial activity against all bacteria tested, except for B.cereus with MIC at a range of 0.37–3.00 mg/mL and MBC at 0.75–6.00 mg/mL. The most potent appeared to be compound 11 with MIC/MBC of0.75/1.5 mg/mL, respectively. The evaluation of antibacterial activity against three resistant strains MRSA, E.coli and P.aeruginosa demonstrated superior activity of compounds against MRSA compared with ampicillin, which did not show bacteriostatic or bactericidal activities. All compounds exhibited good antifungal activity with MIC of 0.37–1.50 mg/mL and MFC of 1.50–3.00 mg/mL, but with different sensitivity against fungi tested. According to docking studies, 14-alpha demethylase inhibition may be responsible for antifungal activity. Two compounds were evaluated for their antibiofilm activity. Finally, drug-likeness and docking prediction were performed.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Antimicrobial Activity of Some Steroidal Hydrazones",
number = "3",
volume = "28",
doi = "10.3390/molecules28031167",
pages = "1167"
}

Merlani, M., Nadaraia, N., Amiranashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J., Carević, T.,& Soković, M.. (2023). Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules
Basel: MDPI., 28(3), 1167.
https://doi.org/10.3390/molecules28031167

Merlani M, Nadaraia N, Amiranashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Carević T, Soković M. Antimicrobial Activity of Some Steroidal Hydrazones. in Molecules. 2023;28(3):1167.
doi:10.3390/molecules28031167 .

Merlani, Maia, Nadaraia, Nanuli, Amiranashvili, Lela, Petrou, Anthi, Geronikaki, Athina, Ćirić, Ana, Glamočlija, Jasmina, Carević, Tamara, Soković, Marina, "Antimicrobial Activity of Some Steroidal Hydrazones" in Molecules, 28, no. 3 (2023):1167,
https://doi.org/10.3390/molecules28031167 . .

TY  - JOUR
AU  - Cavichi, Lucas Vinicius
AU  - Liberal, Ângela
AU  - Dias, Maria Inês
AU  - Mandim, Filipa
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Kalschne, Daneysa Lahis
AU  - Fernandes, Ângela
AU  - Canan, Cristiane
AU  - Barros, Lillian
AU  - Amaral, Joana S.
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/1/192
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36613411
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9818490
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5400
AB  - In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.
PB  - Basel: MDPI
T2  - Foods
T1  - Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil
IS  - 1
VL  - 12
DO  - 10.3390/foods12010192
SP  - 192
ER  - 

@article{
author = "Cavichi, Lucas Vinicius and Liberal, Ângela and Dias, Maria Inês and Mandim, Filipa and Pinela, José and Kostić, Marina and Soković, Marina and Kalschne, Daneysa Lahis and Fernandes, Ângela and Canan, Cristiane and Barros, Lillian and Amaral, Joana S.",
year = "2023",
abstract = "In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.",
publisher = "Basel: MDPI",
journal = "Foods",
title = "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil",
number = "1",
volume = "12",
doi = "10.3390/foods12010192",
pages = "192"
}

Cavichi, L. V., Liberal, Â., Dias, M. I., Mandim, F., Pinela, J., Kostić, M., Soković, M., Kalschne, D. L., Fernandes, Â., Canan, C., Barros, L.,& Amaral, J. S.. (2023). Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods
Basel: MDPI., 12(1), 192.
https://doi.org/10.3390/foods12010192

Cavichi LV, Liberal Â, Dias MI, Mandim F, Pinela J, Kostić M, Soković M, Kalschne DL, Fernandes Â, Canan C, Barros L, Amaral JS. Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods. 2023;12(1):192.
doi:10.3390/foods12010192 .

Cavichi, Lucas Vinicius, Liberal, Ângela, Dias, Maria Inês, Mandim, Filipa, Pinela, José, Kostić, Marina, Soković, Marina, Kalschne, Daneysa Lahis, Fernandes, Ângela, Canan, Cristiane, Barros, Lillian, Amaral, Joana S., "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil" in Foods, 12, no. 1 (2023):192,
https://doi.org/10.3390/foods12010192 . .

TY  - JOUR
AU  - Scanavacca, Juliana
AU  - Bortolucci, Wanessa de Campos
AU  - Jacomassi, Ezilda
AU  - Baretta, Irineia Paulina
AU  - Faria, Maria Graciela Iecher
AU  - Fernandez, Carla Maria Mariano
AU  - Tešević, Vele
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Colauto, Nelson Barros
AU  - Linde, Giani Andrea
AU  - Gazim, Zilda Cristiani
PY  - 2023
UR  - https://blacpma.ms-editions.cl/index.php/blacpma/article/view/337/345
UR  - https://blacpma.ms-editions.cl/index.php/blacpma/article/view/337
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5233
AB  - Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.
PB  - MS-Editions
T2  - Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
T1  - Antimicrobial activity of Tetradenia riparia leaf essential oil
IS  - 2
VL  - 22
DO  - 10.37360/blacpma.23.22.2.19
SP  - 255
EP  - 267
ER  - 

@article{
author = "Scanavacca, Juliana and Bortolucci, Wanessa de Campos and Jacomassi, Ezilda and Baretta, Irineia Paulina and Faria, Maria Graciela Iecher and Fernandez, Carla Maria Mariano and Tešević, Vele and Glamočlija, Jasmina and Soković, Marina and Colauto, Nelson Barros and Linde, Giani Andrea and Gazim, Zilda Cristiani",
year = "2023",
abstract = "Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.",
publisher = "MS-Editions",
journal = "Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas",
title = "Antimicrobial activity of Tetradenia riparia leaf essential oil",
number = "2",
volume = "22",
doi = "10.37360/blacpma.23.22.2.19",
pages = "255-267"
}

Scanavacca, J., Bortolucci, W. d. C., Jacomassi, E., Baretta, I. P., Faria, M. G. I., Fernandez, C. M. M., Tešević, V., Glamočlija, J., Soković, M., Colauto, N. B., Linde, G. A.,& Gazim, Z. C.. (2023). Antimicrobial activity of Tetradenia riparia leaf essential oil. in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
MS-Editions., 22(2), 255-267.
https://doi.org/10.37360/blacpma.23.22.2.19

Scanavacca J, Bortolucci WDC, Jacomassi E, Baretta IP, Faria MGI, Fernandez CMM, Tešević V, Glamočlija J, Soković M, Colauto NB, Linde GA, Gazim ZC. Antimicrobial activity of Tetradenia riparia leaf essential oil. in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas. 2023;22(2):255-267.
doi:10.37360/blacpma.23.22.2.19 .

Scanavacca, Juliana, Bortolucci, Wanessa de Campos, Jacomassi, Ezilda, Baretta, Irineia Paulina, Faria, Maria Graciela Iecher, Fernandez, Carla Maria Mariano, Tešević, Vele, Glamočlija, Jasmina, Soković, Marina, Colauto, Nelson Barros, Linde, Giani Andrea, Gazim, Zilda Cristiani, "Antimicrobial activity of Tetradenia riparia leaf essential oil" in Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 22, no. 2 (2023):255-267,
https://doi.org/10.37360/blacpma.23.22.2.19 . .

TY  - JOUR
AU  - De Oliveira, Izamara
AU  - Chrysargyris, Antonios
AU  - Heleno, Sandrina A.
AU  - Carocho, Marcio
AU  - Calhelha, Ricardo
AU  - Dias, Maria Ines
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
AU  - Santos-Buelga, Celestino
AU  - Tzortzakis, Nikolaos
AU  - Barros, Lillian
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6389
AB  - The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.
PB  - Elsevier
T2  - Food Research International
T1  - Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.
VL  - 170
DO  - 10.1016/j.foodres.2023.113044
SP  - 113044
ER  - 

@article{
author = "De Oliveira, Izamara and Chrysargyris, Antonios and Heleno, Sandrina A. and Carocho, Marcio and Calhelha, Ricardo and Dias, Maria Ines and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon and Santos-Buelga, Celestino and Tzortzakis, Nikolaos and Barros, Lillian",
year = "2023",
abstract = "The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.",
volume = "170",
doi = "10.1016/j.foodres.2023.113044",
pages = "113044"
}

De Oliveira, I., Chrysargyris, A., Heleno, S. A., Carocho, M., Calhelha, R., Dias, M. I., Petrović, J., Soković, M., Petropoulos, S., Santos-Buelga, C., Tzortzakis, N.,& Barros, L.. (2023). Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International
Elsevier., 170, 113044.
https://doi.org/10.1016/j.foodres.2023.113044

De Oliveira I, Chrysargyris A, Heleno SA, Carocho M, Calhelha R, Dias MI, Petrović J, Soković M, Petropoulos S, Santos-Buelga C, Tzortzakis N, Barros L. Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International. 2023;170:113044.
doi:10.1016/j.foodres.2023.113044 .

De Oliveira, Izamara, Chrysargyris, Antonios, Heleno, Sandrina A., Carocho, Marcio, Calhelha, Ricardo, Dias, Maria Ines, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, Santos-Buelga, Celestino, Tzortzakis, Nikolaos, Barros, Lillian, "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes." in Food Research International, 170 (2023):113044,
https://doi.org/10.1016/j.foodres.2023.113044 . .

TY  - JOUR
AU  - Petrou, Anthi
AU  - Kartsev, Victor
AU  - Geronikaki, Athina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5796
AB  - Nine new functionally substituted derivatives of 2-aminothiazole 
were evaluated for antimicrobial activity using microdilution 
method against the panel of eight bacterial and eight fungal strains. 
Evaluation of antibacterial activity revealed that compounds are 
potent antibacterial agents, more active than ampicillin and strep tomycin except of some compounds against B. cereus and En. 
cloacae. The best compound appeared to be compound 8. The 
most sensitive bacteria appeared to be En. cloacae, while L. mono cytogenes was the most resistant. Compounds also exhibited good 
antifungal activity much better than two reference drugs, ketoco nazole and bifonazole. Compound 1 exhibited the best antifungal 
activity. The most sensitive fungus was T. viride, while A. fumigatus 
was the most resistant. Bacteria as well as fungi in general showed 
different sensitivity towards compounds tested. Molecular docking 
studies revealed that MurB inhibition is probably involved in the 
mechanism of antibacterial activity, while CYP51 of C. albicans is 
responsible for the mechanism of antifungal activity. Finally, it 
should be mentioned that all compounds displayed very good 
druglikeness scores
PB  - Taylor & Francis Group
T2  - SAR and QSAR in Environmental Research
T1  - Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation
IS  - 5
VL  - 34
DO  - 10.1080/1062936X.2023.2214869
SP  - 359
EP  - 414
ER  - 

@article{
author = "Petrou, Anthi and Kartsev, Victor and Geronikaki, Athina and Glamočlija, Jasmina and Ćirić, Ana and Soković, Marina",
year = "2023",
abstract = "Nine new functionally substituted derivatives of 2-aminothiazole 
were evaluated for antimicrobial activity using microdilution 
method against the panel of eight bacterial and eight fungal strains. 
Evaluation of antibacterial activity revealed that compounds are 
potent antibacterial agents, more active than ampicillin and strep tomycin except of some compounds against B. cereus and En. 
cloacae. The best compound appeared to be compound 8. The 
most sensitive bacteria appeared to be En. cloacae, while L. mono cytogenes was the most resistant. Compounds also exhibited good 
antifungal activity much better than two reference drugs, ketoco nazole and bifonazole. Compound 1 exhibited the best antifungal 
activity. The most sensitive fungus was T. viride, while A. fumigatus 
was the most resistant. Bacteria as well as fungi in general showed 
different sensitivity towards compounds tested. Molecular docking 
studies revealed that MurB inhibition is probably involved in the 
mechanism of antibacterial activity, while CYP51 of C. albicans is 
responsible for the mechanism of antifungal activity. Finally, it 
should be mentioned that all compounds displayed very good 
druglikeness scores",
publisher = "Taylor & Francis Group",
journal = "SAR and QSAR in Environmental Research",
title = "Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation",
number = "5",
volume = "34",
doi = "10.1080/1062936X.2023.2214869",
pages = "359-414"
}

Petrou, A., Kartsev, V., Geronikaki, A., Glamočlija, J., Ćirić, A.,& Soković, M.. (2023). Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation. in SAR and QSAR in Environmental Research
Taylor & Francis Group., 34(5), 359-414.
https://doi.org/10.1080/1062936X.2023.2214869

Petrou A, Kartsev V, Geronikaki A, Glamočlija J, Ćirić A, Soković M. Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation. in SAR and QSAR in Environmental Research. 2023;34(5):359-414.
doi:10.1080/1062936X.2023.2214869 .

Petrou, Anthi, Kartsev, Victor, Geronikaki, Athina, Glamočlija, Jasmina, Ćirić, Ana, Soković, Marina, "Functionally substituted 2-aminothiazoles as antimicrobial agents: in vitro and in silico evaluation" in SAR and QSAR in Environmental Research, 34, no. 5 (2023):359-414,
https://doi.org/10.1080/1062936X.2023.2214869 . .

TY  - JOUR
AU  - Radovanović, Marina
AU  - Lazarević, Nevena
AU  - Novaković, Jovana
AU  - Jeremić, Nevena
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Bradić, Jovana
AU  - Pecarski, Danijela
AU  - Tel-Çayan, Gülsen
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Gregori, Andrej
AU  - Petrović, Jovana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5792
AB  - The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome
IS  - 6
VL  - 16
DO  - 10.3390/ph16060787
SP  - 787
ER  - 

@article{
author = "Radovanović, Marina and Lazarević, Nevena and Novaković, Jovana and Jeremić, Nevena and Jakovljević, Vladimir and Živković, Vladimir and Bradić, Jovana and Pecarski, Danijela and Tel-Çayan, Gülsen and Glamočlija, Jasmina and Soković, Marina and Gregori, Andrej and Petrović, Jovana",
year = "2023",
abstract = "The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome",
number = "6",
volume = "16",
doi = "10.3390/ph16060787",
pages = "787"
}

Radovanović, M., Lazarević, N., Novaković, J., Jeremić, N., Jakovljević, V., Živković, V., Bradić, J., Pecarski, D., Tel-Çayan, G., Glamočlija, J., Soković, M., Gregori, A.,& Petrović, J.. (2023). Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals
Basel: MDPI., 16(6), 787.
https://doi.org/10.3390/ph16060787

Radovanović M, Lazarević N, Novaković J, Jeremić N, Jakovljević V, Živković V, Bradić J, Pecarski D, Tel-Çayan G, Glamočlija J, Soković M, Gregori A, Petrović J. Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals. 2023;16(6):787.
doi:10.3390/ph16060787 .

Radovanović, Marina, Lazarević, Nevena, Novaković, Jovana, Jeremić, Nevena, Jakovljević, Vladimir, Živković, Vladimir, Bradić, Jovana, Pecarski, Danijela, Tel-Çayan, Gülsen, Glamočlija, Jasmina, Soković, Marina, Gregori, Andrej, Petrović, Jovana, "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome" in Pharmaceuticals, 16, no. 6 (2023):787,
https://doi.org/10.3390/ph16060787 . .

TY  - JOUR
AU  - Albuquerque, Bianca R.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Oliveira, M Beatriz P P
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996923001199
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5481
AB  - Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.
T2  - Food Research International
T1  - Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins
VL  - 165
DO  - 10.1016/j.foodres.2023.112574
SP  - 112574
ER  - 

@article{
author = "Albuquerque, Bianca R. and Pinela, José and Dias, Maria Inês and Pereira, Carla and Petrović, Jovana and Soković, Marina and Calhelha, Ricardo C. and Oliveira, M Beatriz P P and Ferreira, Isabel C F R and Barros, Lillian",
year = "2023",
abstract = "Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.",
journal = "Food Research International",
title = "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins",
volume = "165",
doi = "10.1016/j.foodres.2023.112574",
pages = "112574"
}

Albuquerque, B. R., Pinela, J., Dias, M. I., Pereira, C., Petrović, J., Soković, M., Calhelha, R. C., Oliveira, M. B. P. P., Ferreira, I. C. F. R.,& Barros, L.. (2023). Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International, 165, 112574.
https://doi.org/10.1016/j.foodres.2023.112574

Albuquerque BR, Pinela J, Dias MI, Pereira C, Petrović J, Soković M, Calhelha RC, Oliveira MBPP, Ferreira ICFR, Barros L. Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International. 2023;165:112574.
doi:10.1016/j.foodres.2023.112574 .

Albuquerque, Bianca R., Pinela, José, Dias, Maria Inês, Pereira, Carla, Petrović, Jovana, Soković, Marina, Calhelha, Ricardo C., Oliveira, M Beatriz P P, Ferreira, Isabel C F R, Barros, Lillian, "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins" in Food Research International, 165 (2023):112574,
https://doi.org/10.1016/j.foodres.2023.112574 . .

TY  - JOUR
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Kartsev, Victor
AU  - Kousaxidis, Antonios
AU  - Papadimitriou-Tsantarliotou, Aliki
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Nicolaou, Ioannis
AU  - Vizirianakis, Ioannis S.
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/1/131
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9865890
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5459
AB  - Herein, we report the experimental evaluation of the antimicrobial activity of seventeen new (Z)-methyl 3-(4-oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylate derivatives. All tested compounds exhibited antibacterial activity against eight Gram-positive and Gram-negative bacteria. Their activity exceeded those of ampicillin as well as streptomycin by 10-50 fold. The most sensitive bacterium was En. Cloacae, while E. coli was the most resistant one, followed by M. flavus. The most active compound appeared to be compound 8 with MIC at 0.004-0.03 mg/mL and MBC at 0.008-0.06 mg/mL. The antifungal activity of tested compounds was good to excellent with MIC in the range of 0.004-0.06 mg/mL, with compound 15 being the most potent. T. viride was the most sensitive fungal, while A. fumigatus was the most resistant one. Docking studies revealed that the inhibition of E. coli MurB is probably responsible for their antibacterial activity, while 14a-lanosterol demethylase of CYP51Ca is involved in the mechanism of antifungal activity. Furthermore, drug-likeness and ADMET profile prediction were performed. Finally, the cytotoxicity studies were performed for the most active compounds using MTT assay against normal MRC5 cells.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation
IS  - 1
VL  - 16
DO  - 10.3390/ph16010131
SP  - 131
ER  - 

@article{
author = "Petrou, Anthi and Geronikaki, Athina and Kartsev, Victor and Kousaxidis, Antonios and Papadimitriou-Tsantarliotou, Aliki and Kostić, Marina and Ivanov, Marija and Soković, Marina and Nicolaou, Ioannis and Vizirianakis, Ioannis S.",
year = "2023",
abstract = "Herein, we report the experimental evaluation of the antimicrobial activity of seventeen new (Z)-methyl 3-(4-oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylate derivatives. All tested compounds exhibited antibacterial activity against eight Gram-positive and Gram-negative bacteria. Their activity exceeded those of ampicillin as well as streptomycin by 10-50 fold. The most sensitive bacterium was En. Cloacae, while E. coli was the most resistant one, followed by M. flavus. The most active compound appeared to be compound 8 with MIC at 0.004-0.03 mg/mL and MBC at 0.008-0.06 mg/mL. The antifungal activity of tested compounds was good to excellent with MIC in the range of 0.004-0.06 mg/mL, with compound 15 being the most potent. T. viride was the most sensitive fungal, while A. fumigatus was the most resistant one. Docking studies revealed that the inhibition of E. coli MurB is probably responsible for their antibacterial activity, while 14a-lanosterol demethylase of CYP51Ca is involved in the mechanism of antifungal activity. Furthermore, drug-likeness and ADMET profile prediction were performed. Finally, the cytotoxicity studies were performed for the most active compounds using MTT assay against normal MRC5 cells.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation",
number = "1",
volume = "16",
doi = "10.3390/ph16010131",
pages = "131"
}

Petrou, A., Geronikaki, A., Kartsev, V., Kousaxidis, A., Papadimitriou-Tsantarliotou, A., Kostić, M., Ivanov, M., Soković, M., Nicolaou, I.,& Vizirianakis, I. S.. (2023). N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation. in Pharmaceuticals
Basel: MDPI., 16(1), 131.
https://doi.org/10.3390/ph16010131

Petrou A, Geronikaki A, Kartsev V, Kousaxidis A, Papadimitriou-Tsantarliotou A, Kostić M, Ivanov M, Soković M, Nicolaou I, Vizirianakis IS. N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation. in Pharmaceuticals. 2023;16(1):131.
doi:10.3390/ph16010131 .

Petrou, Anthi, Geronikaki, Athina, Kartsev, Victor, Kousaxidis, Antonios, Papadimitriou-Tsantarliotou, Aliki, Kostić, Marina, Ivanov, Marija, Soković, Marina, Nicolaou, Ioannis, Vizirianakis, Ioannis S., "N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation" in Pharmaceuticals, 16, no. 1 (2023):131,
https://doi.org/10.3390/ph16010131 . .

TY  - JOUR
AU  - Joseph, Blessy
AU  - John, Amrit Jose
AU  - Glamočlija, Jasmina
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Lazović, Saša
AU  - Kochupurackal, Jayachandran
AU  - Kalarikkal, Nandakumar
AU  - Thomas, Sabu
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2352492822018025
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5348
AB  - Electrospun fibers with large surface area are of particular importance in the tissue engineering field as their highly porous structure favours cell adhesion, proliferation, and migration. Herein, we report a facile approach for the development of polycaprolactone (PCL) scaffolds incorporated with zirconium dioxide (ZrO2) nanoparticles. Zirconium dioxide nanoparticles were incorporated into the fibers at five different concentrations (0.5, 1, 3, 5 and 7 wt%), based on the amount of polymer. The morphology of the scaffolds was evaluated by scanning electron microscopy which showed interconnected pore structure. The incorporation of nanoparticles was confirmed from EDX studies. The functional groups were analysed using FTIR and crystallinity was analysed by XRD. Mechanical studies demonstrated that the scaffolds containing 1 wt% Zirconium dioxide nanoparticles exhibited the highest tensile strength. The scaffolds showed antibacterial activity against Gram positive bacterium Staphylococcus lugdunensis and were not active against Gram-negative bacterium Proteus vulgaris, at all time-points. ZrO2 loaded PCL scaffolds with good mechanical and biological properties will open avenues for the potential applications of the electrospun membranes for tissue engineering.
PB  - Elsevier Ltd
T2  - Materials Today Communications
T1  - Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds
VL  - 34
DO  - 10.1016/j.mtcomm.2022.104961
SP  - 104961
ER  - 

@article{
author = "Joseph, Blessy and John, Amrit Jose and Glamočlija, Jasmina and Stojković, Dejan and Soković, Marina and Lazović, Saša and Kochupurackal, Jayachandran and Kalarikkal, Nandakumar and Thomas, Sabu",
year = "2023",
abstract = "Electrospun fibers with large surface area are of particular importance in the tissue engineering field as their highly porous structure favours cell adhesion, proliferation, and migration. Herein, we report a facile approach for the development of polycaprolactone (PCL) scaffolds incorporated with zirconium dioxide (ZrO2) nanoparticles. Zirconium dioxide nanoparticles were incorporated into the fibers at five different concentrations (0.5, 1, 3, 5 and 7 wt%), based on the amount of polymer. The morphology of the scaffolds was evaluated by scanning electron microscopy which showed interconnected pore structure. The incorporation of nanoparticles was confirmed from EDX studies. The functional groups were analysed using FTIR and crystallinity was analysed by XRD. Mechanical studies demonstrated that the scaffolds containing 1 wt% Zirconium dioxide nanoparticles exhibited the highest tensile strength. The scaffolds showed antibacterial activity against Gram positive bacterium Staphylococcus lugdunensis and were not active against Gram-negative bacterium Proteus vulgaris, at all time-points. ZrO2 loaded PCL scaffolds with good mechanical and biological properties will open avenues for the potential applications of the electrospun membranes for tissue engineering.",
publisher = "Elsevier Ltd",
journal = "Materials Today Communications",
title = "Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds",
volume = "34",
doi = "10.1016/j.mtcomm.2022.104961",
pages = "104961"
}

Joseph, B., John, A. J., Glamočlija, J., Stojković, D., Soković, M., Lazović, S., Kochupurackal, J., Kalarikkal, N.,& Thomas, S.. (2023). Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds. in Materials Today Communications
Elsevier Ltd., 34, 104961.
https://doi.org/10.1016/j.mtcomm.2022.104961

Joseph B, John AJ, Glamočlija J, Stojković D, Soković M, Lazović S, Kochupurackal J, Kalarikkal N, Thomas S. Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds. in Materials Today Communications. 2023;34:104961.
doi:10.1016/j.mtcomm.2022.104961 .

Joseph, Blessy, John, Amrit Jose, Glamočlija, Jasmina, Stojković, Dejan, Soković, Marina, Lazović, Saša, Kochupurackal, Jayachandran, Kalarikkal, Nandakumar, Thomas, Sabu, "Processing and evaluation of the structure-properties of electrospun PCL/ zirconium nanoparticle scaffolds" in Materials Today Communications, 34 (2023):104961,
https://doi.org/10.1016/j.mtcomm.2022.104961 . .

TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5140
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Oxford: Elsevier
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
SP  - 134299
ER  - 

@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Oxford: Elsevier",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299",
pages = "134299"
}

Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Oxford: Elsevier., 402, 134299.
https://doi.org/10.1016/j.foodchem.2022.134299

Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402:134299.
doi:10.1016/j.foodchem.2022.134299 .

Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023):134299,
https://doi.org/10.1016/j.foodchem.2022.134299 . .

TY  - JOUR
AU  - Ak, Gunes
AU  - Zengin, Gokhan
AU  - Dall’Acqua, Stefano
AU  - Ferrarese, Irene
AU  - Sut, Stefania
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Nenadić, Marija
AU  - Chiavaroli, Annalisa
AU  - Recinella, Lucia
AU  - Leone, Sheila
AU  - Orlando, Giustino
AU  - Menghini, Luigi
AU  - Ferrante, Claudio
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5069
AB  - The genus Scorzonera is one of the most important genera in the Asteraceae family, and members of the genus have great potential in traditional medicine systems. In this article we aimed to determine skin protective and antimicrobial effects of different extracts from the roots and aerial parts of three Scorzonera species (S. hieraciifolia, S. hispanica, S. tomentosa). Antimicrobial properties were evaluated against different pathogen bacterial and fungal strains, whereas protective effects were studied in isolated mouse skin specimens exposed to hydrogen peroxide. In this biological model, the levels of l-dopa and prostaglandin E2 (PGE2) were evaluated, as markers of tyrosinase activity and inflammation, respectively. All extracts were effective in blunting the increased hydrogen peroxide-induced l-dopa turnover and PGE2 level; thus, suggesting skin protection that could be partly related to the content of flavonoids, among which rutin, in the tested extracts. Additionally, all extracts were effective as antibacterial and antifungal agents; intriguing results were observed against Trichoderma viride, which was particularly sensitive to S. tomentosa root extracts. Therefore, the present study strengthens the importance of investigating herbal preparations obtained from plants belonging to the genus Scorzonera, for the management and prevention of inflammatory and infectious skin conditions.
PB  - Taylor and Francis Ltd.
T2  - Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
T1  - A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa )
IS  - 1
VL  - 157
DO  - 10.1080/11263504.2022.2089766
SP  - 119
EP  - 128
ER  - 

@article{
author = "Ak, Gunes and Zengin, Gokhan and Dall’Acqua, Stefano and Ferrarese, Irene and Sut, Stefania and Glamočlija, Jasmina and Soković, Marina and Nenadić, Marija and Chiavaroli, Annalisa and Recinella, Lucia and Leone, Sheila and Orlando, Giustino and Menghini, Luigi and Ferrante, Claudio",
year = "2023",
abstract = "The genus Scorzonera is one of the most important genera in the Asteraceae family, and members of the genus have great potential in traditional medicine systems. In this article we aimed to determine skin protective and antimicrobial effects of different extracts from the roots and aerial parts of three Scorzonera species (S. hieraciifolia, S. hispanica, S. tomentosa). Antimicrobial properties were evaluated against different pathogen bacterial and fungal strains, whereas protective effects were studied in isolated mouse skin specimens exposed to hydrogen peroxide. In this biological model, the levels of l-dopa and prostaglandin E2 (PGE2) were evaluated, as markers of tyrosinase activity and inflammation, respectively. All extracts were effective in blunting the increased hydrogen peroxide-induced l-dopa turnover and PGE2 level; thus, suggesting skin protection that could be partly related to the content of flavonoids, among which rutin, in the tested extracts. Additionally, all extracts were effective as antibacterial and antifungal agents; intriguing results were observed against Trichoderma viride, which was particularly sensitive to S. tomentosa root extracts. Therefore, the present study strengthens the importance of investigating herbal preparations obtained from plants belonging to the genus Scorzonera, for the management and prevention of inflammatory and infectious skin conditions.",
publisher = "Taylor and Francis Ltd.",
journal = "Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology",
title = "A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa )",
number = "1",
volume = "157",
doi = "10.1080/11263504.2022.2089766",
pages = "119-128"
}

Ak, G., Zengin, G., Dall’Acqua, S., Ferrarese, I., Sut, S., Glamočlija, J., Soković, M., Nenadić, M., Chiavaroli, A., Recinella, L., Leone, S., Orlando, G., Menghini, L.,& Ferrante, C.. (2023). A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa ). in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
Taylor and Francis Ltd.., 157(1), 119-128.
https://doi.org/10.1080/11263504.2022.2089766

Ak G, Zengin G, Dall’Acqua S, Ferrarese I, Sut S, Glamočlija J, Soković M, Nenadić M, Chiavaroli A, Recinella L, Leone S, Orlando G, Menghini L, Ferrante C. A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa ). in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 2023;157(1):119-128.
doi:10.1080/11263504.2022.2089766 .

Ak, Gunes, Zengin, Gokhan, Dall’Acqua, Stefano, Ferrarese, Irene, Sut, Stefania, Glamočlija, Jasmina, Soković, Marina, Nenadić, Marija, Chiavaroli, Annalisa, Recinella, Lucia, Leone, Sheila, Orlando, Giustino, Menghini, Luigi, Ferrante, Claudio, "A new step on the chemical profiles and pharmacological effects of three Scorzonera species ( S. hieraciifolia , S. hispanica and S. tomentosa )" in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 157, no. 1 (2023):119-128,
https://doi.org/10.1080/11263504.2022.2089766 . .

TY  - JOUR
AU  - Fernandes, Angela
AU  - Polyzos, Nikolaos
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6399
AB  - The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants
IS  - 7
VL  - 9
DO  - 10.3390/horticulturae9070838
SP  - 838
ER  - 

@article{
author = "Fernandes, Angela and Polyzos, Nikolaos and Mandim, Filipa and Pereira, Carla and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon",
year = "2023",
abstract = "The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants",
number = "7",
volume = "9",
doi = "10.3390/horticulturae9070838",
pages = "838"
}

Fernandes, A., Polyzos, N., Mandim, F., Pereira, C., Petrović, J., Soković, M.,& Petropoulos, S.. (2023). Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae
Basel: MDPI., 9(7), 838.
https://doi.org/10.3390/horticulturae9070838

Fernandes A, Polyzos N, Mandim F, Pereira C, Petrović J, Soković M, Petropoulos S. Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae. 2023;9(7):838.
doi:10.3390/horticulturae9070838 .

Fernandes, Angela, Polyzos, Nikolaos, Mandim, Filipa, Pereira, Carla, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants" in Horticulturae, 9, no. 7 (2023):838,
https://doi.org/10.3390/horticulturae9070838 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Petrović, Jovana
AU  - Carević, Tamara
AU  - Soković, Marina
AU  - Liaras, Konstantinos
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5793
AB  - This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review
IS  - 6
VL  - 12
DO  - 10.3390/antibiotics12060963
SP  - 963
ER  - 

@article{
author = "Stojković, Dejan and Petrović, Jovana and Carević, Tamara and Soković, Marina and Liaras, Konstantinos",
year = "2023",
abstract = "This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review",
number = "6",
volume = "12",
doi = "10.3390/antibiotics12060963",
pages = "963"
}

Stojković, D., Petrović, J., Carević, T., Soković, M.,& Liaras, K.. (2023). Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics
Basel: MDPI., 12(6), 963.
https://doi.org/10.3390/antibiotics12060963

Stojković D, Petrović J, Carević T, Soković M, Liaras K. Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics. 2023;12(6):963.
doi:10.3390/antibiotics12060963 .

Stojković, Dejan, Petrović, Jovana, Carević, Tamara, Soković, Marina, Liaras, Konstantinos, "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review" in Antibiotics, 12, no. 6 (2023):963,
https://doi.org/10.3390/antibiotics12060963 . .

TY  - JOUR
AU  - Barbakadze, Vakhtang
AU  - Merlani, Maia
AU  - Gogilashvili, Lali
AU  - Amiranashvili, Lela
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2023
UR  - https://www.mdpi.com/2079-6382/12/2/285
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36830198
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9952037
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5491
AB  - This study reports the antimicrobial activities of the biopolymers poly[3-(3,4-dihydoxyphenyl)glyceric acid] (PDHPGA) and poly[2-methoxycarbonyl-3-(3,4-dihydroxyphenyl)oxirane] (PMDHPO), extracted from the six plants of Boraginaceae family: Symphytum asperum (SA), S. caucasicum (SC), S. gr and iflorum (SG), Anchusa italica (AI), Cynoglosum officinale (CO), and Borago officinalis (BO) collected in various parts of Georgia. The study revealed that the antibacterial activities were moderate, and biopolymers from only three plants showed activities against all tested bacteria. Biopolymers from CO stems as well as SC and AI did not show any activity except low activity against a resistant P. aeruginosa strain, which was the most resistant among all three resistant strains. On the other hand, the antifungal activity was better compared to the antibacterial activity. Biopolymers from BO stems exhibited the best activities with MIC/MFC at 0.37-1.00 mg/mL and 0.75-1.5 mg/L, respectively, followed by those from SG stems. Biopolymers from SC and AI roots showed antifungal activities against all six fungi, in contrast to the antibacterial activity, while biopolymers from CO stems and SA roots had activities against four fungi and one fungus, respectively. The sugar-based catechol-containing biopolymers from BO stems demonstrated the best activities among all tested biopolymers against T. viride, P. funiculosum, P. cyclpoium var verucosum, and C. albicans (MIC 0.37 mg/mL). In addition, biopolymers from SG stems were half as active against A. fumigatus and T. viride as ketoconazole. Biopolymers from all plant materials except for CO stems showed higher potency than ketoconazole against T. viride. For the first time, it was shown that all plant materials exhibited better activity against C. albicans, one of the most dreadful fungal species.
PB  - Basel: MDPI
T2  - Antibiotics (Basel, Switzerland)
T1  - Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family
IS  - 2
VL  - 12
DO  - 10.3390/antibiotics12020285
SP  - 285
ER  - 

@article{
author = "Barbakadze, Vakhtang and Merlani, Maia and Gogilashvili, Lali and Amiranashvili, Lela and Petrou, Anthi and Geronikaki, Athina and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2023",
abstract = "This study reports the antimicrobial activities of the biopolymers poly[3-(3,4-dihydoxyphenyl)glyceric acid] (PDHPGA) and poly[2-methoxycarbonyl-3-(3,4-dihydroxyphenyl)oxirane] (PMDHPO), extracted from the six plants of Boraginaceae family: Symphytum asperum (SA), S. caucasicum (SC), S. gr and iflorum (SG), Anchusa italica (AI), Cynoglosum officinale (CO), and Borago officinalis (BO) collected in various parts of Georgia. The study revealed that the antibacterial activities were moderate, and biopolymers from only three plants showed activities against all tested bacteria. Biopolymers from CO stems as well as SC and AI did not show any activity except low activity against a resistant P. aeruginosa strain, which was the most resistant among all three resistant strains. On the other hand, the antifungal activity was better compared to the antibacterial activity. Biopolymers from BO stems exhibited the best activities with MIC/MFC at 0.37-1.00 mg/mL and 0.75-1.5 mg/L, respectively, followed by those from SG stems. Biopolymers from SC and AI roots showed antifungal activities against all six fungi, in contrast to the antibacterial activity, while biopolymers from CO stems and SA roots had activities against four fungi and one fungus, respectively. The sugar-based catechol-containing biopolymers from BO stems demonstrated the best activities among all tested biopolymers against T. viride, P. funiculosum, P. cyclpoium var verucosum, and C. albicans (MIC 0.37 mg/mL). In addition, biopolymers from SG stems were half as active against A. fumigatus and T. viride as ketoconazole. Biopolymers from all plant materials except for CO stems showed higher potency than ketoconazole against T. viride. For the first time, it was shown that all plant materials exhibited better activity against C. albicans, one of the most dreadful fungal species.",
publisher = "Basel: MDPI",
journal = "Antibiotics (Basel, Switzerland)",
title = "Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family",
number = "2",
volume = "12",
doi = "10.3390/antibiotics12020285",
pages = "285"
}

Barbakadze, V., Merlani, M., Gogilashvili, L., Amiranashvili, L., Petrou, A., Geronikaki, A., Ćirić, A., Glamočlija, J.,& Soković, M.. (2023). Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family. in Antibiotics (Basel, Switzerland)
Basel: MDPI., 12(2), 285.
https://doi.org/10.3390/antibiotics12020285

Barbakadze V, Merlani M, Gogilashvili L, Amiranashvili L, Petrou A, Geronikaki A, Ćirić A, Glamočlija J, Soković M. Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family. in Antibiotics (Basel, Switzerland). 2023;12(2):285.
doi:10.3390/antibiotics12020285 .

Barbakadze, Vakhtang, Merlani, Maia, Gogilashvili, Lali, Amiranashvili, Lela, Petrou, Anthi, Geronikaki, Athina, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Antimicrobial Activity of Catechol-Containing Biopolymer Poly[3-(3,4-dihydroxyphenyl)glyceric Acid] from Different Medicinal Plants of Boraginaceae Family" in Antibiotics (Basel, Switzerland), 12, no. 2 (2023):285,
https://doi.org/10.3390/antibiotics12020285 . .

TY  - JOUR
AU  - da Silva, Tamires Barlati Vieira
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Mandim, Filipa
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Seixas, Flávio Augusto Vicente
AU  - Bracht, Adelar
AU  - Peralta, Rosane Marina
PY  - 2023
UR  - http://xlink.rsc.org/?DOI=D2FO02442J
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36723015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5465
AB  - A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.
T2  - Food & Function
T1  - Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice
IS  - 3
VL  - 14
DO  - 10.1039/d2fo02442j
SP  - 1761
EP  - 1772
ER  - 

@article{
author = "da Silva, Tamires Barlati Vieira and Dias, Maria Inês and Pereira, Carla and Mandim, Filipa and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian and Seixas, Flávio Augusto Vicente and Bracht, Adelar and Peralta, Rosane Marina",
year = "2023",
abstract = "A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.",
journal = "Food & Function",
title = "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice",
number = "3",
volume = "14",
doi = "10.1039/d2fo02442j",
pages = "1761-1772"
}

da Silva, T. B. V., Dias, M. I., Pereira, C., Mandim, F., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Barros, L., Seixas, F. A. V., Bracht, A.,& Peralta, R. M.. (2023). Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function, 14(3), 1761-1772.
https://doi.org/10.1039/d2fo02442j

da Silva TBV, Dias MI, Pereira C, Mandim F, Ivanov M, Soković M, Ferreira ICFR, Barros L, Seixas FAV, Bracht A, Peralta RM. Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function. 2023;14(3):1761-1772.
doi:10.1039/d2fo02442j .

da Silva, Tamires Barlati Vieira, Dias, Maria Inês, Pereira, Carla, Mandim, Filipa, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, Seixas, Flávio Augusto Vicente, Bracht, Adelar, Peralta, Rosane Marina, "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice" in Food & Function, 14, no. 3 (2023):1761-1772,
https://doi.org/10.1039/d2fo02442j . .

TY  - JOUR
AU  - Hamad, Mohamad
AU  - AlKhamach, Dana M H
AU  - Alsayadi, Lujain Majed
AU  - Sarhan, Sirin Abdulkafi
AU  - Saeed, Balsam Qubais
AU  - Soković, Marina
AU  - Ben Hadda, Taibi
AU  - Soliman, Sameh S M
PY  - 2023
UR  - https://www.liebertpub.com/doi/10.1089/vim.2022.0122
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5466
AB  - Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) initially emerged in December 2019 and has subsequently expanded globally, leading to the ongoing pandemic. The extensive spread of various SARS-CoV-2 variants possesses a serious public health threat. An extensive literature search along with deep analysis was performed to describe and evaluate the characteristics of SARS-CoV-2 variants of concern in relation to the effectiveness of the current vaccines and therapeutics. The obtained results showed that several significant mutations have evolved during the COVID-19 pandemic. The developed variants and their various structural mutations can compromise the effectiveness of several vaccines, escape the neutralizing antibodies, and limit the efficiency of available therapeutics. Furthermore, deep analysis of the available data enables the prediction of the future impact of virus mutations on the ongoing pandemic along with the selection of appropriate vaccines and therapeutics.
T2  - Viral Immunology
T1  - Alpha to Omicron (Variants of Concern): Mutation Journey, Vaccines, and Therapy
DO  - 10.1089/vim.2022.0122
ER  - 

@article{
author = "Hamad, Mohamad and AlKhamach, Dana M H and Alsayadi, Lujain Majed and Sarhan, Sirin Abdulkafi and Saeed, Balsam Qubais and Soković, Marina and Ben Hadda, Taibi and Soliman, Sameh S M",
year = "2023",
abstract = "Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) initially emerged in December 2019 and has subsequently expanded globally, leading to the ongoing pandemic. The extensive spread of various SARS-CoV-2 variants possesses a serious public health threat. An extensive literature search along with deep analysis was performed to describe and evaluate the characteristics of SARS-CoV-2 variants of concern in relation to the effectiveness of the current vaccines and therapeutics. The obtained results showed that several significant mutations have evolved during the COVID-19 pandemic. The developed variants and their various structural mutations can compromise the effectiveness of several vaccines, escape the neutralizing antibodies, and limit the efficiency of available therapeutics. Furthermore, deep analysis of the available data enables the prediction of the future impact of virus mutations on the ongoing pandemic along with the selection of appropriate vaccines and therapeutics.",
journal = "Viral Immunology",
title = "Alpha to Omicron (Variants of Concern): Mutation Journey, Vaccines, and Therapy",
doi = "10.1089/vim.2022.0122"
}

Hamad, M., AlKhamach, D. M. H., Alsayadi, L. M., Sarhan, S. A., Saeed, B. Q., Soković, M., Ben Hadda, T.,& Soliman, S. S. M.. (2023). Alpha to Omicron (Variants of Concern): Mutation Journey, Vaccines, and Therapy. in Viral Immunology.
https://doi.org/10.1089/vim.2022.0122

Hamad M, AlKhamach DMH, Alsayadi LM, Sarhan SA, Saeed BQ, Soković M, Ben Hadda T, Soliman SSM. Alpha to Omicron (Variants of Concern): Mutation Journey, Vaccines, and Therapy. in Viral Immunology. 2023;.
doi:10.1089/vim.2022.0122 .

Hamad, Mohamad, AlKhamach, Dana M H, Alsayadi, Lujain Majed, Sarhan, Sirin Abdulkafi, Saeed, Balsam Qubais, Soković, Marina, Ben Hadda, Taibi, Soliman, Sameh S M, "Alpha to Omicron (Variants of Concern): Mutation Journey, Vaccines, and Therapy" in Viral Immunology (2023),
https://doi.org/10.1089/vim.2022.0122 . .

TY  - JOUR
AU  - Garcia-Oliveira, Paula
AU  - Carreira-Casais, Anxo
AU  - Pereira, Eliana
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Simal-Gandara, Jesus
AU  - Prieto, Miguel A
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9571014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5170
AB  - Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.
PB  - Basel: MDPI
T2  - Molecules
T1  - From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants
IS  - 19
VL  - 27
DO  - 10.3390/molecules27196495
ER  - 

@article{
author = "Garcia-Oliveira, Paula and Carreira-Casais, Anxo and Pereira, Eliana and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C and Stojković, Dejan and Soković, Marina and Simal-Gandara, Jesus and Prieto, Miguel A and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants",
number = "19",
volume = "27",
doi = "10.3390/molecules27196495"
}

Garcia-Oliveira, P., Carreira-Casais, A., Pereira, E., Dias, M. I., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Simal-Gandara, J., Prieto, M. A., Caleja, C.,& Barros, L.. (2022). From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules
Basel: MDPI., 27(19).
https://doi.org/10.3390/molecules27196495

Garcia-Oliveira P, Carreira-Casais A, Pereira E, Dias MI, Pereira C, Calhelha RC, Stojković D, Soković M, Simal-Gandara J, Prieto MA, Caleja C, Barros L. From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules. 2022;27(19).
doi:10.3390/molecules27196495 .

Garcia-Oliveira, Paula, Carreira-Casais, Anxo, Pereira, Eliana, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C, Stojković, Dejan, Soković, Marina, Simal-Gandara, Jesus, Prieto, Miguel A, Caleja, Cristina, Barros, Lillian, "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196495 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Chaski, Christina
AU  - Pereira, Carla
AU  - Kostić, Marina
AU  - Rouphael, Youssef
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Petropoulos, Spyridon A.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/645
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5090
AB  - The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070645
SP  - 645
ER  - 

@article{
author = "Fernandes, Ângela and Chaski, Christina and Pereira, Carla and Kostić, Marina and Rouphael, Youssef and Soković, Marina and Barros, Lillian and Petropoulos, Spyridon A.",
year = "2022",
abstract = "The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070645",
pages = "645"
}

Fernandes, Â., Chaski, C., Pereira, C., Kostić, M., Rouphael, Y., Soković, M., Barros, L.,& Petropoulos, S. A.. (2022). Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae
Basel: MDPI., 8(7), 645.
https://doi.org/10.3390/horticulturae8070645

Fernandes Â, Chaski C, Pereira C, Kostić M, Rouphael Y, Soković M, Barros L, Petropoulos SA. Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae. 2022;8(7):645.
doi:10.3390/horticulturae8070645 .

Fernandes, Ângela, Chaski, Christina, Pereira, Carla, Kostić, Marina, Rouphael, Youssef, Soković, Marina, Barros, Lillian, Petropoulos, Spyridon A., "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit" in Horticulturae, 8, no. 7 (2022):645,
https://doi.org/10.3390/horticulturae8070645 . .

TY  - JOUR
AU  - Carocho, Márcio
AU  - Barros, Lillian
AU  - Morales, Patricia
AU  - Petropoulos, Spyridon A.
AU  - Soković, Marina
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.1077985/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5339
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Editorial: The chemistry of food in the advent of sustainable diets
VL  - 9
DO  - 10.3389/fnut.2022.1077985
SP  - 1077985
ER  - 

@article{
author = "Carocho, Márcio and Barros, Lillian and Morales, Patricia and Petropoulos, Spyridon A. and Soković, Marina",
year = "2022",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Editorial: The chemistry of food in the advent of sustainable diets",
volume = "9",
doi = "10.3389/fnut.2022.1077985",
pages = "1077985"
}

Carocho, M., Barros, L., Morales, P., Petropoulos, S. A.,& Soković, M.. (2022). Editorial: The chemistry of food in the advent of sustainable diets. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 1077985.
https://doi.org/10.3389/fnut.2022.1077985

Carocho M, Barros L, Morales P, Petropoulos SA, Soković M. Editorial: The chemistry of food in the advent of sustainable diets. in Frontiers in Nutrition. 2022;9:1077985.
doi:10.3389/fnut.2022.1077985 .

Carocho, Márcio, Barros, Lillian, Morales, Patricia, Petropoulos, Spyridon A., Soković, Marina, "Editorial: The chemistry of food in the advent of sustainable diets" in Frontiers in Nutrition, 9 (2022):1077985,
https://doi.org/10.3389/fnut.2022.1077985 . .

TY  - CONF
AU  - Carević, Tamara
AU  - Glamočlija, Jasmina
AU  - Nikolić, Biljana
AU  - Ivanov, Marija
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5332
AB  - The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.
PB  - Novi Sad: Matica srpska
C3  - Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
T1  - Mechanisms of action of selected flavonoids towards species of the genus Candida
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5332
ER  - 

@conference{
author = "Carević, Tamara and Glamočlija, Jasmina and Nikolić, Biljana and Ivanov, Marija and Soković, Marina",
year = "2022",
abstract = "The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.",
publisher = "Novi Sad: Matica srpska",
journal = "Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia",
title = "Mechanisms of action of selected flavonoids towards species of the genus Candida",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5332"
}

Carević, T., Glamočlija, J., Nikolić, B., Ivanov, M.,& Soković, M.. (2022). Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
Novi Sad: Matica srpska., 46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332

Carević T, Glamočlija J, Nikolić B, Ivanov M, Soković M. Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia. 2022;:46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .

Carević, Tamara, Glamočlija, Jasmina, Nikolić, Biljana, Ivanov, Marija, Soković, Marina, "Mechanisms of action of selected flavonoids towards species of the genus Candida" in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia (2022):46,
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .