TY  - DATA
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Lazarević, Jelica
AU  - Todosijević, Marina
AU  - Ljujić, Jovana
AU  - Mitić, Zorica
AU  - Nikolić, Biljana
AU  - Marin, Petar
AU  - Tešević, Vele
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6490
AB  - We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6490
ER  - 

@misc{
author = "Milanović, Slobodan and Milenković, Ivan and Lazarević, Jelica and Todosijević, Marina and Ljujić, Jovana and Mitić, Zorica and Nikolić, Biljana and Marin, Petar and Tešević, Vele",
year = "2024",
abstract = "We provide one excel (with five sheets) and 5 csv files. The data in the excel file is the same as the one provided in the 5 csv files. Data file named 'raw data.xlsx' is consisted of 5 sheets containing raw data on all analyzed traits in the manuscript for the spongy moth and Phytophthora sp. The first sheet named 'RDC' contains data on relative deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The second sheet named 'ADC' contains data on absolute deterrence coefficients of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils. The third sheet named 'RCR' contains data on the relative consumption rate of spongy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The forth sheet named ‘RGR’ contains data on relative growth rate of gypsy moth larvae exposed to three different concentrations of Calocedrus decurrens and Cupressus arizonica essential oils, and control treatment. The fifth sheet named ‘Ir’ contains data on the antimicrobial activity of three concentrations of Calocedrus decurrens and Cupressus arizonica essential oils against Phytophthora × cambivora, P. plurivora and P. quercina expressed as inhibition rate of mycelial growth.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6490"
}

Milanović, S., Milenković, I., Lazarević, J., Todosijević, M., Ljujić, J., Mitić, Z., Nikolić, B., Marin, P.,& Tešević, V.. (2024). Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6490

Milanović S, Milenković I, Lazarević J, Todosijević M, Ljujić J, Mitić Z, Nikolić B, Marin P, Tešević V. Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

Milanović, Slobodan, Milenković, Ivan, Lazarević, Jelica, Todosijević, Marina, Ljujić, Jovana, Mitić, Zorica, Nikolić, Biljana, Marin, Petar, Tešević, Vele, "Biological activity of two coniferous essential oils against spongy moth larvae and Phytophthora root pathogens" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6490 .

TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Uba, Abdullahi Ibrahim
AU  - Zengin, Gokhan
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Ristanović, Elizabeta
AU  - Ćirić, Ana
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/1/21
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9865742
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5433
AB  - This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.
PB  - Basel: MDPI
T2  - Pharmaceuticals (Basel, Switzerland)
T1  - Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts
IS  - 1
VL  - 16
DO  - 10.3390/ph16010021
SP  - 21
ER  - 

@article{
author = "Đorđevski, Nikoleta and Uba, Abdullahi Ibrahim and Zengin, Gokhan and Božunović, Jelena and Gašić, Uroš and Ristanović, Elizabeta and Ćirić, Ana and Nikolić, Biljana and Stojković, Dejan",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals (Basel, Switzerland)",
title = "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts",
number = "1",
volume = "16",
doi = "10.3390/ph16010021",
pages = "21"
}

Đorđevski, N., Uba, A. I., Zengin, G., Božunović, J., Gašić, U., Ristanović, E., Ćirić, A., Nikolić, B.,& Stojković, D.. (2023). Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland)
Basel: MDPI., 16(1), 21.
https://doi.org/10.3390/ph16010021

Đorđevski N, Uba AI, Zengin G, Božunović J, Gašić U, Ristanović E, Ćirić A, Nikolić B, Stojković D. Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland). 2023;16(1):21.
doi:10.3390/ph16010021 .

Đorđevski, Nikoleta, Uba, Abdullahi Ibrahim, Zengin, Gokhan, Božunović, Jelena, Gašić, Uroš, Ristanović, Elizabeta, Ćirić, Ana, Nikolić, Biljana, Stojković, Dejan, "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts" in Pharmaceuticals (Basel, Switzerland), 16, no. 1 (2023):21,
https://doi.org/10.3390/ph16010021 . .

TY  - CONF
AU  - Carević, Tamara
AU  - Glamočlija, Jasmina
AU  - Nikolić, Biljana
AU  - Ivanov, Marija
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5332
AB  - The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.
PB  - Novi Sad: Matica srpska
C3  - Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
T1  - Mechanisms of action of selected flavonoids towards species of the genus Candida
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5332
ER  - 

@conference{
author = "Carević, Tamara and Glamočlija, Jasmina and Nikolić, Biljana and Ivanov, Marija and Soković, Marina",
year = "2022",
abstract = "The frequency of resistance to a large number of antimicrobial agents is increasing, so there
is a need to find new ones. A major problem in the treatment of infections caused by Candida
albicans is a highly resistant biofilm, which is a virulence factor of this species. The
mechanisms of action of selected flavonoids (hesperetin, sakuranetin, and taxifolin) on
species of the genus Candida were explored. The strains that were tested were C. albicans
10/15, C. albicans 13/15, C. albicans ATCC 10231, C. albicans 475/15, C. parapsilosis
ATCC 22019, C. tropicalis ATCC 750, C. krusei H1/16, and C. glabrata 4/6/15. The
antimicrobial potential of flavonoids was investigated by the microdilution method.
Determining the ability of these compounds to disrupt virulence factors, in the model of
Candida albicans, is based on monitoring the process of disrupting the formation and
destruction of previously formed biofilm. The cytotoxic effect on selected human cell lines
(lung fibroblasts) was investigated in order to determine whether flavonoids are selectively
toxic to species of the genus Candida, or cause non-selective toxicity in eukaryotic cells. The
laboratory methods used were microdilution method, antibiofilm method (crystal violet assay,
measures biofilm biomass), Congo red binding assay (measures the influence of compounds
on exopolysaccharide, EPS, production in biofilm), MTT cytotoxicity assay. The most
pronounced antifungal potential was observed for sakuranetin with a MIC of 0.041 mg/mL
for C. parapsilosis ATCC 22019 and a MIC of 0.082 mg/mL for the other strains tested. The
antimicrobial potential of hesperetin and taxifolin is the same for all tested strains; MIC value
is 0.165 mg/mL. All substances have been shown to be effective in inhibiting biofilm
formation, but also in destroying it. The antibiofilm effect of sakuranetin applied in
concentration equal to MIC against C. glabrata 4/6/15 and C. krusei H1/16 is over 85%.
These two strains are the most sensitive to the action of sakuranetin. The ability to destroy the
previously formed biofilm was pronounced towards C. albicans 475/15 (52.80%), and
moderate towards C. albicans ATCC 10231 (12.22%). The antibiofilm potential of hesperetin
is high for C. glabrata 4/6/15 (70.4%) and C. parapsilosis ATCC 22019 (62.6%), while for
other strains the percentage of inhibition is less than 50%, and for the formation of biofilm C.
krusei H1/16 hesperetin has no effect at all. The distinct effect of this flavonoid on the
destruction of previously formed biofilm is emphasized, with significant percentages of
destruction, while the percentages of EPS inhibition are low but significant (higher or closer
to the effect of ketoconazole). The most pronounced antibiofilm effect of taxifolin is
exhibited towards C. glabrata 4/6/15 (61.6%), while the percentage of inhibition is not higher
than 50% for other strains, which is generally lower effect than for other tested substances.
The cytotoxicity test indicates the following range of sensitivity of the examined cell line to
flavonoids: sakuranetin>hesperetin>taxifolin. The tested flavonoids have excellent potential
to be part of antifungal therapies, especially hesperetin, which has shown a strong antibiofilm
effect with pronounced selectivity towards pathogen cells.",
publisher = "Novi Sad: Matica srpska",
journal = "Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia",
title = "Mechanisms of action of selected flavonoids towards species of the genus Candida",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5332"
}

Carević, T., Glamočlija, J., Nikolić, B., Ivanov, M.,& Soković, M.. (2022). Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
Novi Sad: Matica srpska., 46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332

Carević T, Glamočlija J, Nikolić B, Ivanov M, Soković M. Mechanisms of action of selected flavonoids towards species of the genus Candida. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia. 2022;:46.
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .

Carević, Tamara, Glamočlija, Jasmina, Nikolić, Biljana, Ivanov, Marija, Soković, Marina, "Mechanisms of action of selected flavonoids towards species of the genus Candida" in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia (2022):46,
https://hdl.handle.net/21.15107/rcub_ibiss_5332 .

TY  - JOUR
AU  - Carević, Tamara
AU  - Kostić, Marina
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ivanov, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5146
AB  - Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations
PB  - Basel: MDPI
T2  - Molecules
T1  - Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity
IS  - 20
VL  - 27
DO  - 10.3390/molecules27206806
SP  - 6806
ER  - 

@article{
author = "Carević, Tamara and Kostić, Marina and Nikolić, Biljana and Stojković, Dejan and Soković, Marina and Ivanov, Marija",
year = "2022",
abstract = "Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity",
number = "20",
volume = "27",
doi = "10.3390/molecules27206806",
pages = "6806"
}

Carević, T., Kostić, M., Nikolić, B., Stojković, D., Soković, M.,& Ivanov, M.. (2022). Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules
Basel: MDPI., 27(20), 6806.
https://doi.org/10.3390/molecules27206806

Carević T, Kostić M, Nikolić B, Stojković D, Soković M, Ivanov M. Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules. 2022;27(20):6806.
doi:10.3390/molecules27206806 .

Carević, Tamara, Kostić, Marina, Nikolić, Biljana, Stojković, Dejan, Soković, Marina, Ivanov, Marija, "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity" in Molecules, 27, no. 20 (2022):6806,
https://doi.org/10.3390/molecules27206806 . .

TY  - JOUR
AU  - Krstić, Gordana
AU  - Nikolić, Biljana
AU  - Todosijević, Marina
AU  - Mitić, Zorica
AU  - Stankovic-Jeremic, Jovana
AU  - Cvetkovic, Mirjana
AU  - Bojović, Srđan
AU  - Marin, Petar
PY  - 2022
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=1821-21582201039K
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4968
AB  - In this study we have reported the chemical composition of the essential oils obtained from the needles of twelve Pinus taxa, belonging to the subgenera Pinus (sections Pinus and Trifoliae) and Strobus (sect. Quinquefoliae). Monoterpenes dominated over sesquiterpenes in most of the investigated taxa of both subgenera, except in representatives of the subsection Pinaster, subgenus Pinus. alpha-Pinene was the most abundant terpene in eight pines, but other dominant terpenes were found in some taxa -trans-caryophyllene, germacrene D, abietadiene and beta-pinene. In the subsect. Pinaster, the most dominant compounds varied from species to species. In addition, P. halepensis and P. pinaster had the highest percentage of diterpenes, while P. heldreichii was the richest in germacrene D. In the PCA and cluster analyses, three species from the subsect. Pinaster emerged as the most distant: P. halepensis, P. pinaster, and P. heldreichii. In addition, the possible taxonomic implications of the terpene profile in the analysed Pinus taxa were also discussed.
AB  - 48 |vol. 46 (1) U ovom proučavanju objavili smo hemijski sastav etarskih ulja dobijenih iz iglica 12 taksona roda Pinus, koji pripadaju podrodovima Pinus (sekcije Pinus i Trifoliae) i Strobus (sekcija Quinquefoliae). U većini istraživanih taksona monoterpeni dominiraju nad seskviterpenima kod oba podroda, osim kod uzoraka podsekcije Pinaster podroda Pinus. α-Pinen je najobilniji kod 8 borova, ali kod nekih taksona dominantni su trans-kariofilen, germakren D, abietadien i β-pinen. U subcekciji Pinaster dominantne komponente variraju od vrste do vrste. Nadalje, P. ha- lepensis i P. pinaster imaju najveći procenat diterpena, dok je P. heldreichii najbogatiji u germakrenu D. U PCA i klaster analizama pokazale su se najudaljenije tri vrste subsekcije Pinaster: P. halepensis, P. pinaster i P. heldreichii. Nadalje, u analiziranim vrstama roda Pinus diskutovane su moguće taksonomske implikacije terpenskog profila.
T2  - Botanica Serbica
T1  - Terpene relationships among some soft and hard pine species
T1  - Terpenski odnosi između nekih mekih i tvrdih borova
IS  - 1
VL  - 46
DO  - 10.2298/BOTSERB2201039K
SP  - 39
EP  - 48
ER  - 

@article{
author = "Krstić, Gordana and Nikolić, Biljana and Todosijević, Marina and Mitić, Zorica and Stankovic-Jeremic, Jovana and Cvetkovic, Mirjana and Bojović, Srđan and Marin, Petar",
year = "2022",
abstract = "In this study we have reported the chemical composition of the essential oils obtained from the needles of twelve Pinus taxa, belonging to the subgenera Pinus (sections Pinus and Trifoliae) and Strobus (sect. Quinquefoliae). Monoterpenes dominated over sesquiterpenes in most of the investigated taxa of both subgenera, except in representatives of the subsection Pinaster, subgenus Pinus. alpha-Pinene was the most abundant terpene in eight pines, but other dominant terpenes were found in some taxa -trans-caryophyllene, germacrene D, abietadiene and beta-pinene. In the subsect. Pinaster, the most dominant compounds varied from species to species. In addition, P. halepensis and P. pinaster had the highest percentage of diterpenes, while P. heldreichii was the richest in germacrene D. In the PCA and cluster analyses, three species from the subsect. Pinaster emerged as the most distant: P. halepensis, P. pinaster, and P. heldreichii. In addition, the possible taxonomic implications of the terpene profile in the analysed Pinus taxa were also discussed., 48 |vol. 46 (1) U ovom proučavanju objavili smo hemijski sastav etarskih ulja dobijenih iz iglica 12 taksona roda Pinus, koji pripadaju podrodovima Pinus (sekcije Pinus i Trifoliae) i Strobus (sekcija Quinquefoliae). U većini istraživanih taksona monoterpeni dominiraju nad seskviterpenima kod oba podroda, osim kod uzoraka podsekcije Pinaster podroda Pinus. α-Pinen je najobilniji kod 8 borova, ali kod nekih taksona dominantni su trans-kariofilen, germakren D, abietadien i β-pinen. U subcekciji Pinaster dominantne komponente variraju od vrste do vrste. Nadalje, P. ha- lepensis i P. pinaster imaju najveći procenat diterpena, dok je P. heldreichii najbogatiji u germakrenu D. U PCA i klaster analizama pokazale su se najudaljenije tri vrste subsekcije Pinaster: P. halepensis, P. pinaster i P. heldreichii. Nadalje, u analiziranim vrstama roda Pinus diskutovane su moguće taksonomske implikacije terpenskog profila.",
journal = "Botanica Serbica",
title = "Terpene relationships among some soft and hard pine species, Terpenski odnosi između nekih mekih i tvrdih borova",
number = "1",
volume = "46",
doi = "10.2298/BOTSERB2201039K",
pages = "39-48"
}

Krstić, G., Nikolić, B., Todosijević, M., Mitić, Z., Stankovic-Jeremic, J., Cvetkovic, M., Bojović, S.,& Marin, P.. (2022). Terpene relationships among some soft and hard pine species. in Botanica Serbica, 46(1), 39-48.
https://doi.org/10.2298/BOTSERB2201039K

Krstić G, Nikolić B, Todosijević M, Mitić Z, Stankovic-Jeremic J, Cvetkovic M, Bojović S, Marin P. Terpene relationships among some soft and hard pine species. in Botanica Serbica. 2022;46(1):39-48.
doi:10.2298/BOTSERB2201039K .

Krstić, Gordana, Nikolić, Biljana, Todosijević, Marina, Mitić, Zorica, Stankovic-Jeremic, Jovana, Cvetkovic, Mirjana, Bojović, Srđan, Marin, Petar, "Terpene relationships among some soft and hard pine species" in Botanica Serbica, 46, no. 1 (2022):39-48,
https://doi.org/10.2298/BOTSERB2201039K . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Mitić-Ćulafić, Dragana
AU  - Dukanović, Stefana
AU  - Krstić, Aleksandar
AU  - Pavlica, Dušan
AU  - Vlajić, Tamara
AU  - Marković, Dejan
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/0972060X.2022.2040386
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4944
AB  - The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.
PB  - Abingdon: Taylor & Francis Ltd.
T2  - Journal of Essential Oil-Bearing Plants
T1  - Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes
IS  - 1
VL  - 25
DO  - 10.1080/0972060X.2022.2040386
SP  - 111
EP  - 125
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Dukanović, Stefana and Krstić, Aleksandar and Pavlica, Dušan and Vlajić, Tamara and Marković, Dejan",
year = "2022",
abstract = "The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.",
publisher = "Abingdon: Taylor & Francis Ltd.",
journal = "Journal of Essential Oil-Bearing Plants",
title = "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes",
number = "1",
volume = "25",
doi = "10.1080/0972060X.2022.2040386",
pages = "111-125"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Mitić-Ćulafić, D., Dukanović, S., Krstić, A., Pavlica, D., Vlajić, T.,& Marković, D.. (2022). Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants
Abingdon: Taylor & Francis Ltd.., 25(1), 111-125.
https://doi.org/10.1080/0972060X.2022.2040386

Marinković J, Marković T, Nikolić B, Ćirić A, Mitić-Ćulafić D, Dukanović S, Krstić A, Pavlica D, Vlajić T, Marković D. Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants. 2022;25(1):111-125.
doi:10.1080/0972060X.2022.2040386 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Dukanović, Stefana, Krstić, Aleksandar, Pavlica, Dušan, Vlajić, Tamara, Marković, Dejan, "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes" in Journal of Essential Oil-Bearing Plants, 25, no. 1 (2022):111-125,
https://doi.org/10.1080/0972060X.2022.2040386 . .

TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Pljevljakušić, Dejan
AU  - Ristanović, Elizabeta
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fsn3.2777
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4864
AB  - This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.
PB  - John Wiley and Sons Inc
T2  - Food Science & Nutrition
T1  - Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil
IS  - 4
VL  - 10
DO  - 10.1002/fsn3.2777
SP  - 1312
EP  - 1319
ER  - 

@article{
author = "Đorđevski, Nikoleta and Stojković, Dejan and Živković, Jelena and Pljevljakušić, Dejan and Ristanović, Elizabeta and Nikolić, Biljana and Ćirić, Ana",
year = "2022",
abstract = "This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.",
publisher = "John Wiley and Sons Inc",
journal = "Food Science & Nutrition",
title = "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil",
number = "4",
volume = "10",
doi = "10.1002/fsn3.2777",
pages = "1312-1319"
}

Đorđevski, N., Stojković, D., Živković, J., Pljevljakušić, D., Ristanović, E., Nikolić, B.,& Ćirić, A.. (2022). Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition
John Wiley and Sons Inc., 10(4), 1312-1319.
https://doi.org/10.1002/fsn3.2777

Đorđevski N, Stojković D, Živković J, Pljevljakušić D, Ristanović E, Nikolić B, Ćirić A. Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition. 2022;10(4):1312-1319.
doi:10.1002/fsn3.2777 .

Đorđevski, Nikoleta, Stojković, Dejan, Živković, Jelena, Pljevljakušić, Dejan, Ristanović, Elizabeta, Nikolić, Biljana, Ćirić, Ana, "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil" in Food Science & Nutrition, 10, no. 4 (2022):1312-1319,
https://doi.org/10.1002/fsn3.2777 . .

TY  - JOUR
AU  - Popović, Vladan
AU  - Nikolić, Biljana
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Šešlija Jovanović, Darka
AU  - Miljković, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4792
AB  - Geographical and climatic characteristics (elevation, mean annual temperature, summer heat: moisture index, evaporation,
climatic moisture deficit, growing degree-days, heating degree-days, de Martonne’s aridity index) drive variations in
the morphological and anatomical traits of the Norway spruce (Picea abies L. Karst) needle (needle length, needle thickness,
needle width, epidermis thickness with cuticle, hypodermis height, central bundle diameter, resin duct diameter, and the
number of resin ducts). We studied the pattern of variation in these needle characteristics in 15 Balkan Mountains (BM) and
Dinaric Alps (DA) natural populations (altitudes ranging from 1100 to 1860 and from 980 to 1530 m a.s.l., respectively).
The needle traits showed significant differentiation between mountain regions, between populations and within populations.
The largest contribution of genetic component in phenotypic variation had the needle length, both components had the same
share in the needle width, whereas the environmental component of variability had the largest share in the other needle traits.
The principal component analysis revealed that anatomical needle traits and the climatic conditions contributed to differences
between populations from different mountain regions. An agglomerative hierarchical clustering analysis revealed three
dendogram clusters: three BM populations made one cluster, DA populations made the second, whereas seven populations
close to the BM populations and five close to the DA populations made the third cluster. Patterns of morpho-anatomical
phenotypic variability point to selective mechanisms and adjustments, enable to define the boundaries of climate niches,
and may provide a starting point for conservation program according to climate change projections in the Balkan region.
PB  - Berlin : Springer International
T2  - Trees
T1  - Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient
DO  - 10.1007/s00468-022-02277-1
ER  - 

@article{
author = "Popović, Vladan and Nikolić, Biljana and Lučić, Aleksandar and Rakonjac, Ljubinko and Šešlija Jovanović, Darka and Miljković, Danijela",
year = "2022",
abstract = "Geographical and climatic characteristics (elevation, mean annual temperature, summer heat: moisture index, evaporation,
climatic moisture deficit, growing degree-days, heating degree-days, de Martonne’s aridity index) drive variations in
the morphological and anatomical traits of the Norway spruce (Picea abies L. Karst) needle (needle length, needle thickness,
needle width, epidermis thickness with cuticle, hypodermis height, central bundle diameter, resin duct diameter, and the
number of resin ducts). We studied the pattern of variation in these needle characteristics in 15 Balkan Mountains (BM) and
Dinaric Alps (DA) natural populations (altitudes ranging from 1100 to 1860 and from 980 to 1530 m a.s.l., respectively).
The needle traits showed significant differentiation between mountain regions, between populations and within populations.
The largest contribution of genetic component in phenotypic variation had the needle length, both components had the same
share in the needle width, whereas the environmental component of variability had the largest share in the other needle traits.
The principal component analysis revealed that anatomical needle traits and the climatic conditions contributed to differences
between populations from different mountain regions. An agglomerative hierarchical clustering analysis revealed three
dendogram clusters: three BM populations made one cluster, DA populations made the second, whereas seven populations
close to the BM populations and five close to the DA populations made the third cluster. Patterns of morpho-anatomical
phenotypic variability point to selective mechanisms and adjustments, enable to define the boundaries of climate niches,
and may provide a starting point for conservation program according to climate change projections in the Balkan region.",
publisher = "Berlin : Springer International",
journal = "Trees",
title = "Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient",
doi = "10.1007/s00468-022-02277-1"
}

Popović, V., Nikolić, B., Lučić, A., Rakonjac, L., Šešlija Jovanović, D.,& Miljković, D.. (2022). Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient. in Trees
Berlin : Springer International..
https://doi.org/10.1007/s00468-022-02277-1

Popović V, Nikolić B, Lučić A, Rakonjac L, Šešlija Jovanović D, Miljković D. Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient. in Trees. 2022;.
doi:10.1007/s00468-022-02277-1 .

Popović, Vladan, Nikolić, Biljana, Lučić, Aleksandar, Rakonjac, Ljubinko, Šešlija Jovanović, Darka, Miljković, Danijela, "Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient" in Trees (2022),
https://doi.org/10.1007/s00468-022-02277-1 . .

TY  - CONF
AU  - Đorđevski, Nikoleta
AU  - Ćirić, Ana
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5580
AB  - Ова студија дизајнирана је како би се испитао биолошки потенцијал ектраката
цветова Allium scorodoprasum на одређене изолате дерматомицета. Направљено је
девет различитих екстраката кoји су хемијски окарактерисани. Антимикробна
активност испитиваних екстраката тестирана је микродилуционом методом.
Коришћено је десет клиничких изолата дерматомицета изолованих са коже
пацијената (Trichophyton tonsurans, Trichophyton megnini, Trichophyton
mentagrophytes два изолата, Trichophyton rubrum, Microsporum canis два изолата,
Microsporum audouinii два изолата, Epidermophyton floccosum). Екстракти су били
ефикасни против свих тестираних гљива. Најбољу активност према свим
тестираним дерматомицетама показао је екстракт етанол:вода (50:50, v/v). Врсте
рода Microsporum су биле генерално најосетљивије на дејство екстраката. Како
постоји повећана отпорност хуманих патогена на постојеће комерцијалне лекове и
како конвенционални третмани могу имати многа нежељена дејства, у једном
тренутку овакав приступ могао би постати недовољан за лечење. Због овакве
ситуације развој ефикаснијих и мање токсичних антимикробних агенаса,
укључујући природне производе, од круцијалног је значаја. С обзиром да су
екстракти цветова Allium scorodoprasum in vitro показали значајан антифунгални
потенцијал, препоручљиво је њихово даље истраживање у in vivo студијама, а
свакако су потребне и даље клиничке студије.
AB  - Ova studija dizajnirana je kako bi se ispitao biološki potencijal ektrakata cvetova Allium scorodoprasum na određene izolate dermatomiceta. Napravljeno je devet različitih ekstrakata koji su hemijski okarakterisani. Antimikrobna aktivnost ispitivanih ekstrakata testirana je mikrodilucionom metodom. Korišćeno je deset kliničkih izolata dermatomiceta izolovanih sa kože pacijenata (Trichophyton tonsurans, Trichophyton megnini, Trichophyton mentagrophytes dva izolata, Trichophyton rubrum, Microsporum canis dva izolata, Microsporum audouinii dva izolata, Epidermophyton floccosum). Ekstrakti su bili efikasni protiv svih testiranih gljiva. Najbolju aktivnost prema svim testiranim dermatomicetama pokazao je ekstrakt etanol:voda (50:50, v/v). Vrste roda Microsporum su bile generalno najosetljivije na dejstvo ekstrakata. Kako postoji povećana otpornost humanih patogena na postojeće komercijalne lekove i kako konvencionalni tretmani mogu imati mnoga neželjena dejstva, u jednom trenutku ovakav pristup mogao bi postati nedovoljan za lečenje. Zbog ovakve situacije razvoj efikasnijih i manje toksičnih antimikrobnih agenasa, uključujući prirodne proizvode, od krucijalnog je značaja. S obzirom da su ekstrakti cvetova Allium scorodoprasum in vitro pokazali značajan antifungalni potencijal, preporučljivo je njihovo dalje istraživanje u in vivo studijama, a svakako su potrebne i dalje kliničke studije.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета
T1  - Antifungalni potencijal ekstrakata cvetova Allium scorodoprasum na humane izolate dermatomiceta
SP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5580
ER  - 

@conference{
author = "Đorđevski, Nikoleta and Ćirić, Ana and Nikolić, Biljana and Stojković, Dejan",
year = "2022",
abstract = "Ова студија дизајнирана је како би се испитао биолошки потенцијал ектраката
цветова Allium scorodoprasum на одређене изолате дерматомицета. Направљено је
девет различитих екстраката кoји су хемијски окарактерисани. Антимикробна
активност испитиваних екстраката тестирана је микродилуционом методом.
Коришћено је десет клиничких изолата дерматомицета изолованих са коже
пацијената (Trichophyton tonsurans, Trichophyton megnini, Trichophyton
mentagrophytes два изолата, Trichophyton rubrum, Microsporum canis два изолата,
Microsporum audouinii два изолата, Epidermophyton floccosum). Екстракти су били
ефикасни против свих тестираних гљива. Најбољу активност према свим
тестираним дерматомицетама показао је екстракт етанол:вода (50:50, v/v). Врсте
рода Microsporum су биле генерално најосетљивије на дејство екстраката. Како
постоји повећана отпорност хуманих патогена на постојеће комерцијалне лекове и
како конвенционални третмани могу имати многа нежељена дејства, у једном
тренутку овакав приступ могао би постати недовољан за лечење. Због овакве
ситуације развој ефикаснијих и мање токсичних антимикробних агенаса,
укључујући природне производе, од круцијалног је значаја. С обзиром да су
екстракти цветова Allium scorodoprasum in vitro показали значајан антифунгални
потенцијал, препоручљиво је њихово даље истраживање у in vivo студијама, а
свакако су потребне и даље клиничке студије., Ova studija dizajnirana je kako bi se ispitao biološki potencijal ektrakata cvetova Allium scorodoprasum na određene izolate dermatomiceta. Napravljeno je devet različitih ekstrakata koji su hemijski okarakterisani. Antimikrobna aktivnost ispitivanih ekstrakata testirana je mikrodilucionom metodom. Korišćeno je deset kliničkih izolata dermatomiceta izolovanih sa kože pacijenata (Trichophyton tonsurans, Trichophyton megnini, Trichophyton mentagrophytes dva izolata, Trichophyton rubrum, Microsporum canis dva izolata, Microsporum audouinii dva izolata, Epidermophyton floccosum). Ekstrakti su bili efikasni protiv svih testiranih gljiva. Najbolju aktivnost prema svim testiranim dermatomicetama pokazao je ekstrakt etanol:voda (50:50, v/v). Vrste roda Microsporum su bile generalno najosetljivije na dejstvo ekstrakata. Kako postoji povećana otpornost humanih patogena na postojeće komercijalne lekove i kako konvencionalni tretmani mogu imati mnoga neželjena dejstva, u jednom trenutku ovakav pristup mogao bi postati nedovoljan za lečenje. Zbog ovakve situacije razvoj efikasnijih i manje toksičnih antimikrobnih agenasa, uključujući prirodne proizvode, od krucijalnog je značaja. S obzirom da su ekstrakti cvetova Allium scorodoprasum in vitro pokazali značajan antifungalni potencijal, preporučljivo je njihovo dalje istraživanje u in vivo studijama, a svakako su potrebne i dalje kliničke studije.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета, Antifungalni potencijal ekstrakata cvetova Allium scorodoprasum na humane izolate dermatomiceta",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5580"
}

Đorđevski, N., Ćirić, A., Nikolić, B.,& Stojković, D.. (2022). Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 270.
https://hdl.handle.net/21.15107/rcub_ibiss_5580

Đorđevski N, Ćirić A, Nikolić B, Stojković D. Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:270.
https://hdl.handle.net/21.15107/rcub_ibiss_5580 .

Đorđevski, Nikoleta, Ćirić, Ana, Nikolić, Biljana, Stojković, Dejan, "Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):270,
https://hdl.handle.net/21.15107/rcub_ibiss_5580 .

TY  - CONF
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4378
AB  - Essential oils (EOs) are potent antibacterial agents that have numerous applications in different fields, such 
as traditional medicine, pharmacy and cosmetics, food and beverage industry. However, their use in 
dentistry is limited and not adequately explored. In order to investigate the potential to expand their 
applications in dentistry, we screened for antibacterial and antibiofilm activity of selected EOs toward 
Streptococcus salivarius dental isolates. Selected EOs were originated from Pelargonium roseum,
Pelargonium graveolens, Leptospermum petersonii and Eucalyptus citriodora. In order to take insight into 
the variation in sensitivity among bacterial strains of different origin, both isolates from symptomatic and 
asymptomatic root canal infections, as well as the reference S. salivarius ATCC 9222 strain, were involved 
in this study. EOs were chemically characterized by GC/MS-FID analysis. Microdilution assay, was used 
to determine minimal inhibitory concentrations (MICs). Crystal violet (CV) assay was provided to explore 
the potential to disturb biofilm formation. Statistical analysis performed by One Way ANOVA was used to 
compare sensitivity between tested strains.
Chemical characterization revealed that all EOs were abundant in oxygenated monoterpenes (76.8 – 92.4% 
composition). Furthermore, the content of sesquiterpene hydrocarbons was relatively high in P. roseum and 
P. graveolens EOs (12.3% and 16.7%, respectively). The major constituents of Pelargonium oils were 
citronellol, geraniol, citronellyl formate and geranyl formate, contributing together with 71.3% and 57.1% 
of total P. roseum and P. graveolens EOs, respectively. EO of L. petersonii was abundant with following 
dominant constituents: geranial, neral, citronellal, citronellol and isopulegol (accounting for 81.2% of the 
total EO). Some of these constituents, namely citronellal, citronellol and isopulegol, were also dominant in 
E. citriodora oil, constituting 84.8% of its composition. MIC assay demonstrated that referent strain was 
the most sensitive, while the strains isolated from symptomatic infections were highly resistant. Considering 
all tested strains, the comparison of EOs antibacterial activity pointed out the following order, calculated in 
mg mL-1
: E. citriodora (MIC values 0.08±0.03 – 5.42±1.71) > L. petersonii (MIC values 0.53±0.00 –
4.74±0.64) > P. graveolens (MIC values 0.36±0.15 – 5.54±0.00) > P. roseum (MIC values 1.50±0.68 –
13.77±0.69). The inhibition of biofilm formation by EOs was also strain specific; clinical isolates were less 
sensitive than the reference strain (the ranges of biofilm inhibition were 24%-75% and 84%-89%, 
respectively). Similarly as in the microdilution assay, the E. citriodora EO was the most potent biofilm 
inhibitor. 
Taking into account all obtained results, Eucalyptus citriodora EO proved promising antibacterial and 
antibiofilm activity and could be recommended for further investigation as alternative antimicrobial in 
dental practice.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - New insights into anti-streptococcal potential of selected essential oils relevant to dentistry
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4378
ER  - 

@conference{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Soković, Marina and Marković, Dejan",
year = "2021",
abstract = "Essential oils (EOs) are potent antibacterial agents that have numerous applications in different fields, such 
as traditional medicine, pharmacy and cosmetics, food and beverage industry. However, their use in 
dentistry is limited and not adequately explored. In order to investigate the potential to expand their 
applications in dentistry, we screened for antibacterial and antibiofilm activity of selected EOs toward 
Streptococcus salivarius dental isolates. Selected EOs were originated from Pelargonium roseum,
Pelargonium graveolens, Leptospermum petersonii and Eucalyptus citriodora. In order to take insight into 
the variation in sensitivity among bacterial strains of different origin, both isolates from symptomatic and 
asymptomatic root canal infections, as well as the reference S. salivarius ATCC 9222 strain, were involved 
in this study. EOs were chemically characterized by GC/MS-FID analysis. Microdilution assay, was used 
to determine minimal inhibitory concentrations (MICs). Crystal violet (CV) assay was provided to explore 
the potential to disturb biofilm formation. Statistical analysis performed by One Way ANOVA was used to 
compare sensitivity between tested strains.
Chemical characterization revealed that all EOs were abundant in oxygenated monoterpenes (76.8 – 92.4% 
composition). Furthermore, the content of sesquiterpene hydrocarbons was relatively high in P. roseum and 
P. graveolens EOs (12.3% and 16.7%, respectively). The major constituents of Pelargonium oils were 
citronellol, geraniol, citronellyl formate and geranyl formate, contributing together with 71.3% and 57.1% 
of total P. roseum and P. graveolens EOs, respectively. EO of L. petersonii was abundant with following 
dominant constituents: geranial, neral, citronellal, citronellol and isopulegol (accounting for 81.2% of the 
total EO). Some of these constituents, namely citronellal, citronellol and isopulegol, were also dominant in 
E. citriodora oil, constituting 84.8% of its composition. MIC assay demonstrated that referent strain was 
the most sensitive, while the strains isolated from symptomatic infections were highly resistant. Considering 
all tested strains, the comparison of EOs antibacterial activity pointed out the following order, calculated in 
mg mL-1
: E. citriodora (MIC values 0.08±0.03 – 5.42±1.71) > L. petersonii (MIC values 0.53±0.00 –
4.74±0.64) > P. graveolens (MIC values 0.36±0.15 – 5.54±0.00) > P. roseum (MIC values 1.50±0.68 –
13.77±0.69). The inhibition of biofilm formation by EOs was also strain specific; clinical isolates were less 
sensitive than the reference strain (the ranges of biofilm inhibition were 24%-75% and 84%-89%, 
respectively). Similarly as in the microdilution assay, the E. citriodora EO was the most potent biofilm 
inhibitor. 
Taking into account all obtained results, Eucalyptus citriodora EO proved promising antibacterial and 
antibiofilm activity and could be recommended for further investigation as alternative antimicrobial in 
dental practice.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "New insights into anti-streptococcal potential of selected essential oils relevant to dentistry",
pages = "131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4378"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Soković, M.,& Marković, D.. (2021). New insights into anti-streptococcal potential of selected essential oils relevant to dentistry. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 131.
https://hdl.handle.net/21.15107/rcub_ibiss_4378

Marinković J, Marković T, Nikolić B, Ćirić A, Soković M, Marković D. New insights into anti-streptococcal potential of selected essential oils relevant to dentistry. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:131.
https://hdl.handle.net/21.15107/rcub_ibiss_4378 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Soković, Marina, Marković, Dejan, "New insights into anti-streptococcal potential of selected essential oils relevant to dentistry" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):131,
https://hdl.handle.net/21.15107/rcub_ibiss_4378 .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Soldatović, Ivan
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Marković, Dejan
PY  - 2021
UR  - https://www.tandfonline.com/doi/abs/10.1080/0972060X.2021.1899059
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4245
AB  - The study aimed to investigate the potency of Leptospermum petersonii, Eucalyptus citriodora, Pelargonium graveolens, and Pelargonium roseum essential oils (EOs) against Streptococcus sanguinis and Streptococcus salivarius dental isolates. To examine the variation in sensitivity among strains of different origin, isolates from symptomatic and asymptomatic root canal infections, as well as the reference, strains S. sanguinis ATCC 10556 and S. salivarius ATCC 9222 were used in the study. EOs were chemically characterised (GC/MS). Their antibacterial and antibiofilm properties were detected by microdilution and CV assay respectively. Statistical analysis was performed by One way ANOVA. Examined bacterial strains, regardless clinical or ATCC, exhibited the highest sensitivity to L. petersonii and E. citriodora EOs (MIC ranged 0.53±0.00 - 5.88±1.27 mg/ml and 0.08±0.03 - 9.37±1.25 mg/ml respectively), while two other EOs were less efficient. Comparison of MIC values showed that sensitivity of the isolates recovered from symptomatic infections was the least, while the susceptibility of asymptomatic isolates and especially of reference strains were higher. EO inhibition of biofilm formation differed between clinical isolates of S. salivarius and their reference strain. Inhibition was higher in the case of S. salivarius ATCC 9222 (84 %-89 %) in comparison to that of clinical isolates (24 %-75 %). Regardless of the strains tested, E. citriodora exhibited the highest antibiofilm potential. To conclude: L. petersonii induced notable antibacterial potential, while E. citriodora EO proved promising antibacterial and antibiofilm activity. Due to the variable sensitivity of the isolates to EOs, it is suggested to screen activity on a large number of strains, especially from symptomatic infections.
PB  - Informa UK Limited
T2  - Journal of Essential Oil Bearing Plants
T1  - Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates
IS  - 2
VL  - 24
DO  - 10.1080/0972060x.2021.1899059
SP  - 304
EP  - 316
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Soldatović, Ivan and Ivanov, Marija and Ćirić, Ana and Soković, Marina and Marković, Dejan",
year = "2021",
abstract = "The study aimed to investigate the potency of Leptospermum petersonii, Eucalyptus citriodora, Pelargonium graveolens, and Pelargonium roseum essential oils (EOs) against Streptococcus sanguinis and Streptococcus salivarius dental isolates. To examine the variation in sensitivity among strains of different origin, isolates from symptomatic and asymptomatic root canal infections, as well as the reference, strains S. sanguinis ATCC 10556 and S. salivarius ATCC 9222 were used in the study. EOs were chemically characterised (GC/MS). Their antibacterial and antibiofilm properties were detected by microdilution and CV assay respectively. Statistical analysis was performed by One way ANOVA. Examined bacterial strains, regardless clinical or ATCC, exhibited the highest sensitivity to L. petersonii and E. citriodora EOs (MIC ranged 0.53±0.00 - 5.88±1.27 mg/ml and 0.08±0.03 - 9.37±1.25 mg/ml respectively), while two other EOs were less efficient. Comparison of MIC values showed that sensitivity of the isolates recovered from symptomatic infections was the least, while the susceptibility of asymptomatic isolates and especially of reference strains were higher. EO inhibition of biofilm formation differed between clinical isolates of S. salivarius and their reference strain. Inhibition was higher in the case of S. salivarius ATCC 9222 (84 %-89 %) in comparison to that of clinical isolates (24 %-75 %). Regardless of the strains tested, E. citriodora exhibited the highest antibiofilm potential. To conclude: L. petersonii induced notable antibacterial potential, while E. citriodora EO proved promising antibacterial and antibiofilm activity. Due to the variable sensitivity of the isolates to EOs, it is suggested to screen activity on a large number of strains, especially from symptomatic infections.",
publisher = "Informa UK Limited",
journal = "Journal of Essential Oil Bearing Plants",
title = "Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates",
number = "2",
volume = "24",
doi = "10.1080/0972060x.2021.1899059",
pages = "304-316"
}

Marinković, J., Marković, T., Nikolić, B., Soldatović, I., Ivanov, M., Ćirić, A., Soković, M.,& Marković, D.. (2021). Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates. in Journal of Essential Oil Bearing Plants
Informa UK Limited., 24(2), 304-316.
https://doi.org/10.1080/0972060x.2021.1899059

Marinković J, Marković T, Nikolić B, Soldatović I, Ivanov M, Ćirić A, Soković M, Marković D. Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates. in Journal of Essential Oil Bearing Plants. 2021;24(2):304-316.
doi:10.1080/0972060x.2021.1899059 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Soldatović, Ivan, Ivanov, Marija, Ćirić, Ana, Soković, Marina, Marković, Dejan, "Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates" in Journal of Essential Oil Bearing Plants, 24, no. 2 (2021):304-316,
https://doi.org/10.1080/0972060x.2021.1899059 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Nikolić, Biljana
AU  - Marković, Tatjana
AU  - Radulović, Milena
AU  - Ilić, Jugoslav
AU  - Bošković, Marko
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4360
AB  - The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus
oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and an tibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization
of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination
of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by
2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm
efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU count ing assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed
biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential.
Both substances induced some antioxidant activity. Conclusion: Results encourage further research with
the aim of application of the nanoemulsion in dental practice.
PB  - London: Future Medicine Ltd.
T2  - Future Microbiology
T1  - Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm
IS  - 12
VL  - 16
DO  - 10.2217/fmb-2021-0081
SP  - 907
EP  - 918
ER  - 

@article{
author = "Marinković, Jelena and Nikolić, Biljana and Marković, Tatjana and Radulović, Milena and Ilić, Jugoslav and Bošković, Marko and Ćirić, Ana and Marković, Dejan",
year = "2021",
abstract = "The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus
oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and an tibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization
of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination
of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by
2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm
efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU count ing assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed
biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential.
Both substances induced some antioxidant activity. Conclusion: Results encourage further research with
the aim of application of the nanoemulsion in dental practice.",
publisher = "London: Future Medicine Ltd.",
journal = "Future Microbiology",
title = "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm",
number = "12",
volume = "16",
doi = "10.2217/fmb-2021-0081",
pages = "907-918"
}

Marinković, J., Nikolić, B., Marković, T., Radulović, M., Ilić, J., Bošković, M., Ćirić, A.,& Marković, D.. (2021). Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology
London: Future Medicine Ltd.., 16(12), 907-918.
https://doi.org/10.2217/fmb-2021-0081

Marinković J, Nikolić B, Marković T, Radulović M, Ilić J, Bošković M, Ćirić A, Marković D. Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology. 2021;16(12):907-918.
doi:10.2217/fmb-2021-0081 .

Marinković, Jelena, Nikolić, Biljana, Marković, Tatjana, Radulović, Milena, Ilić, Jugoslav, Bošković, Marko, Ćirić, Ana, Marković, Dejan, "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm" in Future Microbiology, 16, no. 12 (2021):907-918,
https://doi.org/10.2217/fmb-2021-0081 . .

TY  - CONF
AU  - Ćirić, Ana
AU  - Džamić, Ana
AU  - Žarković, Lazar
AU  - Nikolić, Biljana
AU  - Kostić, Marina
AU  - Adakalić, Mirjana
AU  - Lazović, Biljana
AU  - Perović, Tatjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4375
AB  - Jujube (Zizifus jujuba Mill., Rhamnaceae) is a warm climate plant. The fruit has high energy and nutritional 
value, contains a lot of sugar, dietary fiber, vitamin C, minerals, phenolic compounds. Therefore, this plant 
is a valuable source of phytochemicals, bioactives, vitamins etc. which makes it to be effective in curing or 
prevention from various diseases. The present work investigate quantification of total phenols and 
flavonoids, antioxidant and antimicrobial potential of different extracts Z. jujuba, originating from different 
localities of Montenegro (Podgorica, Ulcinj, and Bar). Four different dry extracts were extracted from dried 
fruits, by ultrasound assisted extraction using various solvents (methanol, water, dichloromethane and n butanol). Quantification of total phenols and flavonoids was done by spectrophotometric methods. The 
results showed that these extracts are rich in phenolic compounds, but do not contain a high percentage of 
flavonoids. The antioxidant activity of the extracts was performed by DPPH and ABTS method. The best 
antioxidant potential in the DPPH test was shown for n-butanol extracts, while in the ABTS test methanol, 
dichloromethane and aqueous extracts were shown to be significantly better antioxidants. The antimicrobial 
effect of Z. jujuba extracts was tested on seven bacterial species, belonging to human infectious agents and 
food contaminants and four clinical isolates of dermatomycetes, by microdilution method. Based on the 
results, all extracts possessed antibacterial/antifungal activity against tested bacteria and fungi. The extracts 
showed very high antimicrobial potential, with the aqueous extract being slightly weaker compared to the 
other three ones. In addition to small differences in the effect of the extracts, resistant Escherichia coli (R) 
proved to be the most resistant bacteria, with MIC of 0.19 mg/mL and a MBC of 0.38 mg/mL. Tested 
extracts possessed similar activates against dermatomycetes, but n-butanol extracts had the best potential 
(MIC and MFC values was in the ranges of 0.045-0.38 and 0.09-0.75 mg/mL, respectively). Also, there is 
no difference in antifungal potential according to the origin of the extracts. The extracts from all three 
localities show approximately the same antifungal effect. Microsporum canis proved to be the most sensitive 
fungus, whose growth was inhibited at very low concentrations (MIC 0.09-0.19 mg/mL and MFC 0.19-0.38 
mg/mL). 
The obtained results show that fruit of Z. jujuba and its extracts represent a potential source of new 
compounds with antioxidant and antimicrobial activity. It is an appropriate candidate for treatment of 
microbial infections and for therapeutic application.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - In vitro assay of bioactive potential of Ziziphus jujuba Mill. extracts
SP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4375
ER  - 

@conference{
author = "Ćirić, Ana and Džamić, Ana and Žarković, Lazar and Nikolić, Biljana and Kostić, Marina and Adakalić, Mirjana and Lazović, Biljana and Perović, Tatjana",
year = "2021",
abstract = "Jujube (Zizifus jujuba Mill., Rhamnaceae) is a warm climate plant. The fruit has high energy and nutritional 
value, contains a lot of sugar, dietary fiber, vitamin C, minerals, phenolic compounds. Therefore, this plant 
is a valuable source of phytochemicals, bioactives, vitamins etc. which makes it to be effective in curing or 
prevention from various diseases. The present work investigate quantification of total phenols and 
flavonoids, antioxidant and antimicrobial potential of different extracts Z. jujuba, originating from different 
localities of Montenegro (Podgorica, Ulcinj, and Bar). Four different dry extracts were extracted from dried 
fruits, by ultrasound assisted extraction using various solvents (methanol, water, dichloromethane and n butanol). Quantification of total phenols and flavonoids was done by spectrophotometric methods. The 
results showed that these extracts are rich in phenolic compounds, but do not contain a high percentage of 
flavonoids. The antioxidant activity of the extracts was performed by DPPH and ABTS method. The best 
antioxidant potential in the DPPH test was shown for n-butanol extracts, while in the ABTS test methanol, 
dichloromethane and aqueous extracts were shown to be significantly better antioxidants. The antimicrobial 
effect of Z. jujuba extracts was tested on seven bacterial species, belonging to human infectious agents and 
food contaminants and four clinical isolates of dermatomycetes, by microdilution method. Based on the 
results, all extracts possessed antibacterial/antifungal activity against tested bacteria and fungi. The extracts 
showed very high antimicrobial potential, with the aqueous extract being slightly weaker compared to the 
other three ones. In addition to small differences in the effect of the extracts, resistant Escherichia coli (R) 
proved to be the most resistant bacteria, with MIC of 0.19 mg/mL and a MBC of 0.38 mg/mL. Tested 
extracts possessed similar activates against dermatomycetes, but n-butanol extracts had the best potential 
(MIC and MFC values was in the ranges of 0.045-0.38 and 0.09-0.75 mg/mL, respectively). Also, there is 
no difference in antifungal potential according to the origin of the extracts. The extracts from all three 
localities show approximately the same antifungal effect. Microsporum canis proved to be the most sensitive 
fungus, whose growth was inhibited at very low concentrations (MIC 0.09-0.19 mg/mL and MFC 0.19-0.38 
mg/mL). 
The obtained results show that fruit of Z. jujuba and its extracts represent a potential source of new 
compounds with antioxidant and antimicrobial activity. It is an appropriate candidate for treatment of 
microbial infections and for therapeutic application.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "In vitro assay of bioactive potential of Ziziphus jujuba Mill. extracts",
pages = "102",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4375"
}

Ćirić, A., Džamić, A., Žarković, L., Nikolić, B., Kostić, M., Adakalić, M., Lazović, B.,& Perović, T.. (2021). In vitro assay of bioactive potential of Ziziphus jujuba Mill. extracts. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 102.
https://hdl.handle.net/21.15107/rcub_ibiss_4375

Ćirić A, Džamić A, Žarković L, Nikolić B, Kostić M, Adakalić M, Lazović B, Perović T. In vitro assay of bioactive potential of Ziziphus jujuba Mill. extracts. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:102.
https://hdl.handle.net/21.15107/rcub_ibiss_4375 .

Ćirić, Ana, Džamić, Ana, Žarković, Lazar, Nikolić, Biljana, Kostić, Marina, Adakalić, Mirjana, Lazović, Biljana, Perović, Tatjana, "In vitro assay of bioactive potential of Ziziphus jujuba Mill. extracts" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):102,
https://hdl.handle.net/21.15107/rcub_ibiss_4375 .

TY  - CONF
AU  - Nikolić, Biljana
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Radulović, Milena
AU  - Mitić Ćulafić, Dragana
AU  - Đukanović, Stefana
AU  - Ćirić, Ana
AU  - Tasić, Gvozden
AU  - Bošković, Marko
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4376
AB  - The interest in natural products applications as antimicrobial agents is rapidly growing, mainly due to 
increasing microbial resistance to conventional therapeutics. Essential oils (EOs) seem to be especially 
attractive, since many of them possess high antimicrobial potential and none revealed resistance issue. EOs 
have already found applications in endodontic practice, mainly as sealers, but their antimicrobial potential 
has not been explored in this field yet. Taking this into account, the objective of this study was to investigate 
the antibacterial and antibiofilm efficacy of Cymbopogon martinii EO against bacterial strains relevant for 
endodontic therapy. Chemical composition of EO was determined by GC-MS/FID analysis. Antibacterial 
potential was screened in microdilution assay, detecting minimal inhibitory and bactericidal concentrations 
(MICs and MBCs) and performed on Enterococcus faecalis, Streptococcus mitis and Streptococcus 
sanguinis. Eradicating potential against biofilms preformed of each bacterial species as well as of all three 
bacteria, was determined in vitro in crystal violet (CV) assay. For screening of in situ effect against biofilm 
preformed in root canals of extracted teeth, the EO-based irrigant was formulated. Its antibiofilm potential 
was evaluated by plate counting (PC) assay, performed with single E. faecalis biofilm and multispecies 
biofilm composed of all three strains. Single E. faecalis biofilm was selected since it is the most relevant 
intracanal pathogen, while multispecies biofilm was screened to better mimic clinical conditions.
Chemical analysis revealed that geraniol was the most abundant constituent (83.5%), followed by geranyl 
acetate (8.4%). Determined MIC and MBC values were the lowest for E. faecalis (0.12 and 0.25 mg mL-1
, 
respectively), and the highest for S. sanguinis (2.95 and 5.90 mg mL-1
, respectively). CV assay pointed out 
that EO successfully eradicated in vitro preformed biofilms of E. faecalis and S. mitis (approximately 30% 
reduction), but had no effect on S. sanguinis and multispecies biofilms. EO-based irrigant used for 
determination of in situ antibiofilm potential was pre-screened for its physicochemical characteristics: a 
mean particle diameter and polydispersity index was 101 ± 3 nm and 0.36 ± 0.06, respectively. PC assay 
showed that eradicating potential of applied irrigant was weak against both E. faecalis and multispecies 
intracanal biofilm (reduction of logCFU was 0.76 and 0.52, respectively). However, successive irrigation 
with conventional dental irrigants (sodium hypochlorite and sterile saline), followed by C. martini EO-based 
irrigant, notably increased the biofilm reduction (1.89 and 1.69, respectively).
In conclusion, C. martini EO possesses notable antibacterial potential that could be further investigated for 
different dental applications.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4376
ER  - 

@conference{
author = "Nikolić, Biljana and Marinković, Jelena and Marković, Tatjana and Radulović, Milena and Mitić Ćulafić, Dragana and Đukanović, Stefana and Ćirić, Ana and Tasić, Gvozden and Bošković, Marko and Marković, Dejan",
year = "2021",
abstract = "The interest in natural products applications as antimicrobial agents is rapidly growing, mainly due to 
increasing microbial resistance to conventional therapeutics. Essential oils (EOs) seem to be especially 
attractive, since many of them possess high antimicrobial potential and none revealed resistance issue. EOs 
have already found applications in endodontic practice, mainly as sealers, but their antimicrobial potential 
has not been explored in this field yet. Taking this into account, the objective of this study was to investigate 
the antibacterial and antibiofilm efficacy of Cymbopogon martinii EO against bacterial strains relevant for 
endodontic therapy. Chemical composition of EO was determined by GC-MS/FID analysis. Antibacterial 
potential was screened in microdilution assay, detecting minimal inhibitory and bactericidal concentrations 
(MICs and MBCs) and performed on Enterococcus faecalis, Streptococcus mitis and Streptococcus 
sanguinis. Eradicating potential against biofilms preformed of each bacterial species as well as of all three 
bacteria, was determined in vitro in crystal violet (CV) assay. For screening of in situ effect against biofilm 
preformed in root canals of extracted teeth, the EO-based irrigant was formulated. Its antibiofilm potential 
was evaluated by plate counting (PC) assay, performed with single E. faecalis biofilm and multispecies 
biofilm composed of all three strains. Single E. faecalis biofilm was selected since it is the most relevant 
intracanal pathogen, while multispecies biofilm was screened to better mimic clinical conditions.
Chemical analysis revealed that geraniol was the most abundant constituent (83.5%), followed by geranyl 
acetate (8.4%). Determined MIC and MBC values were the lowest for E. faecalis (0.12 and 0.25 mg mL-1
, 
respectively), and the highest for S. sanguinis (2.95 and 5.90 mg mL-1
, respectively). CV assay pointed out 
that EO successfully eradicated in vitro preformed biofilms of E. faecalis and S. mitis (approximately 30% 
reduction), but had no effect on S. sanguinis and multispecies biofilms. EO-based irrigant used for 
determination of in situ antibiofilm potential was pre-screened for its physicochemical characteristics: a 
mean particle diameter and polydispersity index was 101 ± 3 nm and 0.36 ± 0.06, respectively. PC assay 
showed that eradicating potential of applied irrigant was weak against both E. faecalis and multispecies 
intracanal biofilm (reduction of logCFU was 0.76 and 0.52, respectively). However, successive irrigation 
with conventional dental irrigants (sodium hypochlorite and sterile saline), followed by C. martini EO-based 
irrigant, notably increased the biofilm reduction (1.89 and 1.69, respectively).
In conclusion, C. martini EO possesses notable antibacterial potential that could be further investigated for 
different dental applications.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4376"
}

Nikolić, B., Marinković, J., Marković, T., Radulović, M., Mitić Ćulafić, D., Đukanović, S., Ćirić, A., Tasić, G., Bošković, M.,& Marković, D.. (2021). Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_4376

Nikolić B, Marinković J, Marković T, Radulović M, Mitić Ćulafić D, Đukanović S, Ćirić A, Tasić G, Bošković M, Marković D. Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_4376 .

Nikolić, Biljana, Marinković, Jelena, Marković, Tatjana, Radulović, Milena, Mitić Ćulafić, Dragana, Đukanović, Stefana, Ćirić, Ana, Tasić, Gvozden, Bošković, Marko, Marković, Dejan, "Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):109,
https://hdl.handle.net/21.15107/rcub_ibiss_4376 .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Tešević, Vele
AU  - Đorđević, Iris
AU  - Todosijević, Marina
AU  - Mitić, Zorica
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3710
AB  - The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) analyses of two-year-old needles were performed us-ing an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8 % on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5 % on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains
IS  - 1
VL  - 39
DO  - 10.20450/mjcce.2020.1951
SP  - 41
EP  - 48
ER  - 

@article{
author = "Nikolić, Biljana and Tešević, Vele and Đorđević, Iris and Todosijević, Marina and Mitić, Zorica and Bojović, Srđan and Marin, Petar D.",
year = "2020",
abstract = "The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) analyses of two-year-old needles were performed us-ing an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8 % on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5 % on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains",
number = "1",
volume = "39",
doi = "10.20450/mjcce.2020.1951",
pages = "41-48"
}

Nikolić, B., Tešević, V., Đorđević, I., Todosijević, M., Mitić, Z., Bojović, S.,& Marin, P. D.. (2020). Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 39(1), 41-48.
https://doi.org/10.20450/mjcce.2020.1951

Nikolić B, Tešević V, Đorđević I, Todosijević M, Mitić Z, Bojović S, Marin PD. Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains. in Macedonian Journal of Chemistry and Chemical Engineering. 2020;39(1):41-48.
doi:10.20450/mjcce.2020.1951 .

Nikolić, Biljana, Tešević, Vele, Đorđević, Iris, Todosijević, Marina, Mitić, Zorica, Bojović, Srđan, Marin, Petar D., "Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains" in Macedonian Journal of Chemistry and Chemical Engineering, 39, no. 1 (2020):41-48,
https://doi.org/10.20450/mjcce.2020.1951 . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Ljujić, Jovana
AU  - Bojović, Srđan
AU  - Mitić, Zorica
AU  - Rajčević, Nemanja
AU  - Tešević, Vele
AU  - Marin, Petar
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000038N
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4040
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5348
AB  - The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Headspace volatiles isolated from twigs of Picea omorika from Serbia
IS  - 3
VL  - 72
DO  - 10.2298/ABS200511038N
SP  - 445
EP  - 452
ER  - 

@article{
author = "Nikolić, Biljana and Ljujić, Jovana and Bojović, Srđan and Mitić, Zorica and Rajčević, Nemanja and Tešević, Vele and Marin, Petar",
year = "2020",
abstract = "The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Headspace volatiles isolated from twigs of Picea omorika from Serbia",
number = "3",
volume = "72",
doi = "10.2298/ABS200511038N",
pages = "445-452"
}

Nikolić, B., Ljujić, J., Bojović, S., Mitić, Z., Rajčević, N., Tešević, V.,& Marin, P.. (2020). Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences
Serbian Biological Society., 72(3), 445-452.
https://doi.org/10.2298/ABS200511038N

Nikolić B, Ljujić J, Bojović S, Mitić Z, Rajčević N, Tešević V, Marin P. Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences. 2020;72(3):445-452.
doi:10.2298/ABS200511038N .

Nikolić, Biljana, Ljujić, Jovana, Bojović, Srđan, Mitić, Zorica, Rajčević, Nemanja, Tešević, Vele, Marin, Petar, "Headspace volatiles isolated from twigs of Picea omorika from Serbia" in Archives of Biological Sciences, 72, no. 3 (2020):445-452,
https://doi.org/10.2298/ABS200511038N . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Mitić Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Đukanović, Stefana
AU  - Marković, Tatjana
AU  - Tasić, Gvozden
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32707220
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3831
AB  - OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.
PB  - Elsevier Ltd
T2  - Archives of Oral Biology
T1  - Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.
VL  - 117
DO  - 10.1016/j.archoralbio.2020.104842
SP  - 104842
ER  - 

@article{
author = "Marinković, Jelena and Mitić Ćulafić, Dragana and Nikolić, Biljana and Đukanović, Stefana and Marković, Tatjana and Tasić, Gvozden and Ćirić, Ana and Marković, Dejan",
year = "2020",
abstract = "OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.",
publisher = "Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.",
volume = "117",
doi = "10.1016/j.archoralbio.2020.104842",
pages = "104842"
}

Marinković, J., Mitić Ćulafić, D., Nikolić, B., Đukanović, S., Marković, T., Tasić, G., Ćirić, A.,& Marković, D.. (2020). Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology
Elsevier Ltd., 117, 104842.
https://doi.org/10.1016/j.archoralbio.2020.104842

Marinković J, Mitić Ćulafić D, Nikolić B, Đukanović S, Marković T, Tasić G, Ćirić A, Marković D. Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology. 2020;117:104842.
doi:10.1016/j.archoralbio.2020.104842 .

Marinković, Jelena, Mitić Ćulafić, Dragana, Nikolić, Biljana, Đukanović, Stefana, Marković, Tatjana, Tasić, Gvozden, Ćirić, Ana, Marković, Dejan, "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals." in Archives of Oral Biology, 117 (2020):104842,
https://doi.org/10.1016/j.archoralbio.2020.104842 . .

TY  - JOUR
AU  - Cvetković, Stefana
AU  - Todorović, Slađana
AU  - Nastasijević, Branislav
AU  - Mitić-Ćulafić, Dragana
AU  - Đukanović, Stefana
AU  - Knežević-Vukčević, Jelena
AU  - Nikolić, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3712
AB  - Medicinal plant Gentiana lutea is considered as an official drug for gastrointestinal disorders in many pharmacopoeias. Its uncontrolled overexploitation led to the protection regime and encouraged development of in vitro culture. The aim of this work was to chemically characterize root and leaf methanol extracts of plant grown in plantation and in vitro conditions, and to determine their antigenotoxicity against heterocyclic aromatic amines IQ and PhIP, the food-borne mutagens that are formed during high temperature cooking of protein-reach foods. High yields and growth ratios were determined for in vitro plants. Higher content of polyphenols and flavonoids were determined in aerial parts, while UPLC-MS/MS analysis pointed at the richness of in vitro grown shoot extract (GLvS) in active constituents, and high amounts of gentiopicroside in all tested extracts. Notable amounts of sweroside were detected in plantation root extract (GLR) and in GLvS, and of loganic acid in plantation leaf extract (GLL) and GLvS. Two experimental models for genotoxicity/antigenotoxicity study, i.e. SOS/umuC test with metabolic activation (addition of rat liver S9 fraction) and alkaline comet assay on hepatocellur carcinoma HepG2 cells indicated dual effects: genotoxic of high and antigenotoxic of lower non-genotoxic doses. While in vitro extracts were genotoxic in both models, plantation extracts were active only in SOS/umuC with S9. In contrast, inhibition of genotoxicity was observed in both models for all extracts (up to 83 %). Results of DPPH assay indicated that antioxidativity could be responsible for antigenotoxic properties. In conclusion, genoprotective effect encourages further investigation of antigenotoxicity, while high growth ratio and richness of GLvS in active compounds justify the use of in vitro cultivation method.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro
VL  - 154
DO  - 10.1016/j.indcrop.2020.112690
SP  - 112690
ER  - 

@article{
author = "Cvetković, Stefana and Todorović, Slađana and Nastasijević, Branislav and Mitić-Ćulafić, Dragana and Đukanović, Stefana and Knežević-Vukčević, Jelena and Nikolić, Biljana",
year = "2020",
abstract = "Medicinal plant Gentiana lutea is considered as an official drug for gastrointestinal disorders in many pharmacopoeias. Its uncontrolled overexploitation led to the protection regime and encouraged development of in vitro culture. The aim of this work was to chemically characterize root and leaf methanol extracts of plant grown in plantation and in vitro conditions, and to determine their antigenotoxicity against heterocyclic aromatic amines IQ and PhIP, the food-borne mutagens that are formed during high temperature cooking of protein-reach foods. High yields and growth ratios were determined for in vitro plants. Higher content of polyphenols and flavonoids were determined in aerial parts, while UPLC-MS/MS analysis pointed at the richness of in vitro grown shoot extract (GLvS) in active constituents, and high amounts of gentiopicroside in all tested extracts. Notable amounts of sweroside were detected in plantation root extract (GLR) and in GLvS, and of loganic acid in plantation leaf extract (GLL) and GLvS. Two experimental models for genotoxicity/antigenotoxicity study, i.e. SOS/umuC test with metabolic activation (addition of rat liver S9 fraction) and alkaline comet assay on hepatocellur carcinoma HepG2 cells indicated dual effects: genotoxic of high and antigenotoxic of lower non-genotoxic doses. While in vitro extracts were genotoxic in both models, plantation extracts were active only in SOS/umuC with S9. In contrast, inhibition of genotoxicity was observed in both models for all extracts (up to 83 %). Results of DPPH assay indicated that antioxidativity could be responsible for antigenotoxic properties. In conclusion, genoprotective effect encourages further investigation of antigenotoxicity, while high growth ratio and richness of GLvS in active compounds justify the use of in vitro cultivation method.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro",
volume = "154",
doi = "10.1016/j.indcrop.2020.112690",
pages = "112690"
}

Cvetković, S., Todorović, S., Nastasijević, B., Mitić-Ćulafić, D., Đukanović, S., Knežević-Vukčević, J.,& Nikolić, B.. (2020). Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro. in Industrial Crops and Products
Elsevier B.V.., 154, 112690.
https://doi.org/10.1016/j.indcrop.2020.112690

Cvetković S, Todorović S, Nastasijević B, Mitić-Ćulafić D, Đukanović S, Knežević-Vukčević J, Nikolić B. Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro. in Industrial Crops and Products. 2020;154:112690.
doi:10.1016/j.indcrop.2020.112690 .

Cvetković, Stefana, Todorović, Slađana, Nastasijević, Branislav, Mitić-Ćulafić, Dragana, Đukanović, Stefana, Knežević-Vukčević, Jelena, Nikolić, Biljana, "Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro" in Industrial Crops and Products, 154 (2020):112690,
https://doi.org/10.1016/j.indcrop.2020.112690 . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Vasilijević, Bojana
AU  - Ćirić, Ana
AU  - Mitić-Ćulafić, Dragana
AU  - Cvetković, Stefana
AU  - Džamić, Ana
AU  - Knežević-Vukčević, Jelena
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900005N
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3760
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3401
AB  - Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1, respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC50 values were 0.087-0.106 and 1.450-6.840 mg mL-1, respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesion of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis
T2  - Archives of Biological Sciences
T1  - Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants
IS  - 2
VL  - 71
DO  - 10.2298/ABS181217005N
SP  - 235
EP  - 244
ER  - 

@article{
author = "Nikolić, Biljana and Vasilijević, Bojana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Cvetković, Stefana and Džamić, Ana and Knežević-Vukčević, Jelena",
year = "2019",
abstract = "Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1, respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC50 values were 0.087-0.106 and 1.450-6.840 mg mL-1, respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesion of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis",
journal = "Archives of Biological Sciences",
title = "Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants",
number = "2",
volume = "71",
doi = "10.2298/ABS181217005N",
pages = "235-244"
}

Nikolić, B., Vasilijević, B., Ćirić, A., Mitić-Ćulafić, D., Cvetković, S., Džamić, A.,& Knežević-Vukčević, J.. (2019). Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants. in Archives of Biological Sciences, 71(2), 235-244.
https://doi.org/10.2298/ABS181217005N

Nikolić B, Vasilijević B, Ćirić A, Mitić-Ćulafić D, Cvetković S, Džamić A, Knežević-Vukčević J. Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants. in Archives of Biological Sciences. 2019;71(2):235-244.
doi:10.2298/ABS181217005N .

Nikolić, Biljana, Vasilijević, Bojana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Cvetković, Stefana, Džamić, Ana, Knežević-Vukčević, Jelena, "Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants" in Archives of Biological Sciences, 71, no. 2 (2019):235-244,
https://doi.org/10.2298/ABS181217005N . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Mitić, Zorica
AU  - Bojović, Srđan
AU  - Matevski, Vlado
AU  - Krivošej, Zoran
AU  - Marin, Petar
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3630
AB  - Eight morpho-anatomical properties of two-year-old needles of Pinus heldreichii (Bosnian pine) from the Scardo-Pindic mountain massif in Serbia (Kosovo, Mt. Ošljak) and North Macedonia (Mt. Gali.ica) were investigated. All measured characteristics, except for needle length, were inspected on mid-needle cross-section. Cross-sections were obtained with razorblade, while measurements were performed with Leica-Gallen III light microscope. The mean values of the analyzed characters were as follows: 5.91 cm (needle length), 1.35 mm (needle width), 0.85 mm (needle thickness), 25.05 μm (cuticle + epidermis thickness), 69.90 μm (height of hypodermal cells), 21.76 μm (resin duct diameter), 3.4 (number of hypodermis layers), and 3.6 (number of resin ducts). P. heldreichii needles also had 2-5 hypodermis layers and 0-12 resin ducts. The highest variation was in the number of resin ducts (CV=27%). In comparison with previously investigated needles from the Dinaric mountains, P. heldreichii needles from the Scardo-Pindic massif were shorter and had a thicker layer of hypodermis. PCA and CA visualize partial segregation of P. heldreichii populations between the two mountain massifs.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Variability of needle morpho-anatomy of natural pinus heldreichii populations from scardo-pindic mountains
IS  - 3
VL  - 51
DO  - 10.2298/GENSR1903175N
SP  - 1175
EP  - 1184
ER  - 

@article{
author = "Nikolić, Biljana and Mitić, Zorica and Bojović, Srđan and Matevski, Vlado and Krivošej, Zoran and Marin, Petar",
year = "2019",
abstract = "Eight morpho-anatomical properties of two-year-old needles of Pinus heldreichii (Bosnian pine) from the Scardo-Pindic mountain massif in Serbia (Kosovo, Mt. Ošljak) and North Macedonia (Mt. Gali.ica) were investigated. All measured characteristics, except for needle length, were inspected on mid-needle cross-section. Cross-sections were obtained with razorblade, while measurements were performed with Leica-Gallen III light microscope. The mean values of the analyzed characters were as follows: 5.91 cm (needle length), 1.35 mm (needle width), 0.85 mm (needle thickness), 25.05 μm (cuticle + epidermis thickness), 69.90 μm (height of hypodermal cells), 21.76 μm (resin duct diameter), 3.4 (number of hypodermis layers), and 3.6 (number of resin ducts). P. heldreichii needles also had 2-5 hypodermis layers and 0-12 resin ducts. The highest variation was in the number of resin ducts (CV=27%). In comparison with previously investigated needles from the Dinaric mountains, P. heldreichii needles from the Scardo-Pindic massif were shorter and had a thicker layer of hypodermis. PCA and CA visualize partial segregation of P. heldreichii populations between the two mountain massifs.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Variability of needle morpho-anatomy of natural pinus heldreichii populations from scardo-pindic mountains",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903175N",
pages = "1175-1184"
}

Nikolić, B., Mitić, Z., Bojović, S., Matevski, V., Krivošej, Z.,& Marin, P.. (2019). Variability of needle morpho-anatomy of natural pinus heldreichii populations from scardo-pindic mountains. in Genetika
Serbian Genetics Society., 51(3), 1175-1184.
https://doi.org/10.2298/GENSR1903175N

Nikolić B, Mitić Z, Bojović S, Matevski V, Krivošej Z, Marin P. Variability of needle morpho-anatomy of natural pinus heldreichii populations from scardo-pindic mountains. in Genetika. 2019;51(3):1175-1184.
doi:10.2298/GENSR1903175N .

Nikolić, Biljana, Mitić, Zorica, Bojović, Srđan, Matevski, Vlado, Krivošej, Zoran, Marin, Petar, "Variability of needle morpho-anatomy of natural pinus heldreichii populations from scardo-pindic mountains" in Genetika, 51, no. 3 (2019):1175-1184,
https://doi.org/10.2298/GENSR1903175N . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Kovačević, Dragan
AU  - Mladenović-Drinić, Snežana
AU  - Nikolić, Ana
AU  - Mitić, Zorica
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0534-00121801069N
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3088
AB  - Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.
T2  - Genetika
T1  - Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites
IS  - 1
VL  - 50
DO  - 10.2298/GENSR1801069N
SP  - 69
EP  - 84
ER  - 

@article{
author = "Nikolić, Biljana and Kovačević, Dragan and Mladenović-Drinić, Snežana and Nikolić, Ana and Mitić, Zorica and Bojović, Srđan and Marin, Petar D.",
year = "2018",
abstract = "Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.",
journal = "Genetika",
title = "Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites",
number = "1",
volume = "50",
doi = "10.2298/GENSR1801069N",
pages = "69-84"
}

Nikolić, B., Kovačević, D., Mladenović-Drinić, S., Nikolić, A., Mitić, Z., Bojović, S.,& Marin, P. D.. (2018). Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites. in Genetika, 50(1), 69-84.
https://doi.org/10.2298/GENSR1801069N

Nikolić B, Kovačević D, Mladenović-Drinić S, Nikolić A, Mitić Z, Bojović S, Marin PD. Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites. in Genetika. 2018;50(1):69-84.
doi:10.2298/GENSR1801069N .

Nikolić, Biljana, Kovačević, Dragan, Mladenović-Drinić, Snežana, Nikolić, Ana, Mitić, Zorica, Bojović, Srđan, Marin, Petar D., "Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites" in Genetika, 50, no. 1 (2018):69-84,
https://doi.org/10.2298/GENSR1801069N . .

TY  - GEN
AU  - Marković, Milena
AU  - Matić, Rada
AU  - Vidaković, Vera
AU  - Bojović, Srđan
AU  - Nikolić, Biljana
AU  - Popović, Zorica
PY  - 2017
UR  - http://link.springer.com/10.1007/s00468-017-1579-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2807
AB  - Key message: Multivariate morphometric analyses were used for the first time to examine sexual dimorphism and shape variability of needles in natural populations of relict speciesTaxus baccataL.Abstract: Needle morphology and intraspecific variability of Taxus baccata L. were examined for the first time in Serbian natural populations (in the central Balkans). A geometric morphometric approach was used to assess size and shape variation of needles and to address questions of sexual dimorphism and phenotypic plasticity. Population-specific sexual size dimorphism was found. In the Tara population, females had larger needles than males and this was in accordance with earlier reports on T. baccata dimorphism. We found it unusual that in the other two populations, Kopaonik and Malinik, sexual size dimorphism was constrained and the sexes had needles of equal size. Multivariate analysis of needle shape revealed that the Tara population differed from the other two populations and was characterized by straighter, more symmetric needles. The absence of sexual size dimorphism and the less symmetrical shape of needles could be signs of a deviation from optimal conditions for yew in the Kopaonik and Malinik populations as regards mean annual temperature, precipitation, humidity and soil quality. There is also an indication that sexual size dimorphism in needles arises from a modification in female morphology and that female yews carry more information about climate. However, the conclusions presented in this study have certain limitations because of the small number of individuals in the Tara population (only 3 female and 4 male plants) that represent the quasi-total of adult yews at this site.
T2  - Trees
T1  - Exploration of sexual dimorphism of Taxus baccata L. needles in natural populations
DO  - 10.1007/s00468-017-1579-6
ER  - 

@misc{
author = "Marković, Milena and Matić, Rada and Vidaković, Vera and Bojović, Srđan and Nikolić, Biljana and Popović, Zorica",
year = "2017",
abstract = "Key message: Multivariate morphometric analyses were used for the first time to examine sexual dimorphism and shape variability of needles in natural populations of relict speciesTaxus baccataL.Abstract: Needle morphology and intraspecific variability of Taxus baccata L. were examined for the first time in Serbian natural populations (in the central Balkans). A geometric morphometric approach was used to assess size and shape variation of needles and to address questions of sexual dimorphism and phenotypic plasticity. Population-specific sexual size dimorphism was found. In the Tara population, females had larger needles than males and this was in accordance with earlier reports on T. baccata dimorphism. We found it unusual that in the other two populations, Kopaonik and Malinik, sexual size dimorphism was constrained and the sexes had needles of equal size. Multivariate analysis of needle shape revealed that the Tara population differed from the other two populations and was characterized by straighter, more symmetric needles. The absence of sexual size dimorphism and the less symmetrical shape of needles could be signs of a deviation from optimal conditions for yew in the Kopaonik and Malinik populations as regards mean annual temperature, precipitation, humidity and soil quality. There is also an indication that sexual size dimorphism in needles arises from a modification in female morphology and that female yews carry more information about climate. However, the conclusions presented in this study have certain limitations because of the small number of individuals in the Tara population (only 3 female and 4 male plants) that represent the quasi-total of adult yews at this site.",
journal = "Trees",
title = "Exploration of sexual dimorphism of Taxus baccata L. needles in natural populations",
doi = "10.1007/s00468-017-1579-6"
}

Marković, M., Matić, R., Vidaković, V., Bojović, S., Nikolić, B.,& Popović, Z.. (2017). Exploration of sexual dimorphism of Taxus baccata L. needles in natural populations. in Trees.
https://doi.org/10.1007/s00468-017-1579-6

Marković M, Matić R, Vidaković V, Bojović S, Nikolić B, Popović Z. Exploration of sexual dimorphism of Taxus baccata L. needles in natural populations. in Trees. 2017;.
doi:10.1007/s00468-017-1579-6 .

Marković, Milena, Matić, Rada, Vidaković, Vera, Bojović, Srđan, Nikolić, Biljana, Popović, Zorica, "Exploration of sexual dimorphism of Taxus baccata L. needles in natural populations" in Trees (2017),
https://doi.org/10.1007/s00468-017-1579-6 . .

TY  - GEN
AU  - Bojović, Srđan
AU  - Naydenov, Krassimir D.
AU  - Naydenov, Michel K.
AU  - Alexandrov, Alexander
AU  - Vasilevski, Kole
AU  - Hinkov, Georgi
AU  - Matevski, Vlado
AU  - Nikolić, Biljana
AU  - Goudiaby, Venceslas
AU  - Riegert, Dave
AU  - Paitaridou, Despina
AU  - Christou, Andreas
AU  - Goia, Irina
AU  - Carcaillet, Christopher
AU  - Escudero Alcantara, Adrian
AU  - Ture, Cengiz
AU  - Gulcu, Suleyman
AU  - Gyuleva, Veselka
AU  - Peruzzi, Lorenzo
AU  - Kamary, Salim
AU  - Tsarev, Anatoly
AU  - Bogunic, Faruk
PY  - 2017
UR  - http://link.springer.com/10.1007/s10342-017-1069-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2845
AB  - The historical changes in European Black Pine population size across the whole natural distribution in Europe and Asia Minor were analyzed facing the Plio-Pleistocene climatic fluctuations. Thirteen chloroplast SSRs and SNPs markers have been studied under the assumptions of “neutral evolution.” Populations and meta-populations had different histories of migration routes, and they were strongly affected by complex patterns of isolation, fragmentation, speciation, expansion (1.88–4.28 Ma), purification selection (2.09–21.41 Ma) and bottleneck (1.85–21.76 Ma). A significant number of populations (min. 29–41%) were in equilibrium for very long periods. Generally, the bottleneck revealed by chloroplast DNA is weaker than the bottleneck revealed by nuclear DNA. The N e immediately after the bottleneck reaches between 1820 and 3640 individuals. Generally, the historical effective population sizes shrink significantly for the Tertiary period from 10–15 up to 2.5 Ma in Western Europe (by 82%), followed by Asia Minor (69%) and the Balkan Peninsula (28%), likely resulting from important climatic changes. The rates and frequencies of stepwise westwards migration waves have been not sufficient to prevent isolation between the meta-populations and to suppress “sympatric speciation.” The migration was weak for the Pliocene, but was maximal for the Pleistocene, and finally silent for the present interglacial period, namely the Holocene.
T2  - European Journal of Forest Research
T1  - Ancient genetic bottleneck and Plio-Pleistocene climatic changes imprinted the phylobiogeography of European Black Pine populations
DO  - 10.1007/s10342-017-1069-9
ER  - 

@misc{
author = "Bojović, Srđan and Naydenov, Krassimir D. and Naydenov, Michel K. and Alexandrov, Alexander and Vasilevski, Kole and Hinkov, Georgi and Matevski, Vlado and Nikolić, Biljana and Goudiaby, Venceslas and Riegert, Dave and Paitaridou, Despina and Christou, Andreas and Goia, Irina and Carcaillet, Christopher and Escudero Alcantara, Adrian and Ture, Cengiz and Gulcu, Suleyman and Gyuleva, Veselka and Peruzzi, Lorenzo and Kamary, Salim and Tsarev, Anatoly and Bogunic, Faruk",
year = "2017",
abstract = "The historical changes in European Black Pine population size across the whole natural distribution in Europe and Asia Minor were analyzed facing the Plio-Pleistocene climatic fluctuations. Thirteen chloroplast SSRs and SNPs markers have been studied under the assumptions of “neutral evolution.” Populations and meta-populations had different histories of migration routes, and they were strongly affected by complex patterns of isolation, fragmentation, speciation, expansion (1.88–4.28 Ma), purification selection (2.09–21.41 Ma) and bottleneck (1.85–21.76 Ma). A significant number of populations (min. 29–41%) were in equilibrium for very long periods. Generally, the bottleneck revealed by chloroplast DNA is weaker than the bottleneck revealed by nuclear DNA. The N e immediately after the bottleneck reaches between 1820 and 3640 individuals. Generally, the historical effective population sizes shrink significantly for the Tertiary period from 10–15 up to 2.5 Ma in Western Europe (by 82%), followed by Asia Minor (69%) and the Balkan Peninsula (28%), likely resulting from important climatic changes. The rates and frequencies of stepwise westwards migration waves have been not sufficient to prevent isolation between the meta-populations and to suppress “sympatric speciation.” The migration was weak for the Pliocene, but was maximal for the Pleistocene, and finally silent for the present interglacial period, namely the Holocene.",
journal = "European Journal of Forest Research",
title = "Ancient genetic bottleneck and Plio-Pleistocene climatic changes imprinted the phylobiogeography of European Black Pine populations",
doi = "10.1007/s10342-017-1069-9"
}

Bojović, S., Naydenov, K. D., Naydenov, M. K., Alexandrov, A., Vasilevski, K., Hinkov, G., Matevski, V., Nikolić, B., Goudiaby, V., Riegert, D., Paitaridou, D., Christou, A., Goia, I., Carcaillet, C., Escudero Alcantara, A., Ture, C., Gulcu, S., Gyuleva, V., Peruzzi, L., Kamary, S., Tsarev, A.,& Bogunic, F.. (2017). Ancient genetic bottleneck and Plio-Pleistocene climatic changes imprinted the phylobiogeography of European Black Pine populations. in European Journal of Forest Research.
https://doi.org/10.1007/s10342-017-1069-9

Bojović S, Naydenov KD, Naydenov MK, Alexandrov A, Vasilevski K, Hinkov G, Matevski V, Nikolić B, Goudiaby V, Riegert D, Paitaridou D, Christou A, Goia I, Carcaillet C, Escudero Alcantara A, Ture C, Gulcu S, Gyuleva V, Peruzzi L, Kamary S, Tsarev A, Bogunic F. Ancient genetic bottleneck and Plio-Pleistocene climatic changes imprinted the phylobiogeography of European Black Pine populations. in European Journal of Forest Research. 2017;.
doi:10.1007/s10342-017-1069-9 .

Bojović, Srđan, Naydenov, Krassimir D., Naydenov, Michel K., Alexandrov, Alexander, Vasilevski, Kole, Hinkov, Georgi, Matevski, Vlado, Nikolić, Biljana, Goudiaby, Venceslas, Riegert, Dave, Paitaridou, Despina, Christou, Andreas, Goia, Irina, Carcaillet, Christopher, Escudero Alcantara, Adrian, Ture, Cengiz, Gulcu, Suleyman, Gyuleva, Veselka, Peruzzi, Lorenzo, Kamary, Salim, Tsarev, Anatoly, Bogunic, Faruk, "Ancient genetic bottleneck and Plio-Pleistocene climatic changes imprinted the phylobiogeography of European Black Pine populations" in European Journal of Forest Research (2017),
https://doi.org/10.1007/s10342-017-1069-9 . .

TY  - CONF
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Lakić, Iva
AU  - Vujović, Predrag
AU  - Đorđević, Jelena
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Pavlović, Slađan
AU  - Prokić, Marko
AU  - Zaletel, Ivan
AU  - Todorović, Zoran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3422
AB  - We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat glucose homeostasis. Animals were divided into two groups with 6 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. We measured fasting glycemia once a week during the entire experiment. In the last week of the experiment, we performed an oral glucose tolerance test (OGTT) and an intraperitoneal insulin tolerance test (ITT). On the last day of the experiment the fasting insulin and glyc8mia were measured in the blood of animals. The results show that coconut oil reduces weekly glycemia in VCO animals compared with controls. This effect reaches its maximum after the first two weeks of the experiment, and then slowly decreases and disappears over time of next eight weeks. As a result, the glycemia of control and VCO animals do not differ in last six weeks of the experiment. The area under the curve (AUC) presenting glycemia during whole the experiment is significantly lower in VCO animals than in the controls. The hypoglycemic effect of coconut oil is obviously dose-dependent since the amount of food (and therefore the coconut oil) that the animals eat decreases over the time. The results of the oral glucose tolerance test show that the OGTT AUC of VCO animals is significantly lower than the controls, and same is true for the insulin tolerance test. Finally, glycemia and insulin concentration in serums sampled on the last day of the experiment do not differ between VCO and Control groups, so accordingly neither HOMA-IR I and 2 (Homeostatic model assessment of insulin resistance) nor QUIC.Kl ( Quantitative Insulin Sensitivity Check Index). In conclusion, our results show beneficial effects of long-term high-dose coconut oil supplementation on rat glucose homeostasis.
PB  - BIT Congress Inc. (BIT Group Global Ltd.)
C3  - BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
T1  - The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis
SP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3422
ER  - 

@conference{
author = "Đurašević, Siniša and Jasnić, Nebojša and Dakić, Tamara and Jevđović, Tanja and Lakić, Iva and Vujović, Predrag and Đorđević, Jelena and Mitić-Ćulafić, Dragana and Nikolić, Biljana and Grigorov, Ilijana and Bogojević, Desanka and Pavlović, Slađan and Prokić, Marko and Zaletel, Ivan and Todorović, Zoran",
year = "2017",
abstract = "We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat glucose homeostasis. Animals were divided into two groups with 6 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. We measured fasting glycemia once a week during the entire experiment. In the last week of the experiment, we performed an oral glucose tolerance test (OGTT) and an intraperitoneal insulin tolerance test (ITT). On the last day of the experiment the fasting insulin and glyc8mia were measured in the blood of animals. The results show that coconut oil reduces weekly glycemia in VCO animals compared with controls. This effect reaches its maximum after the first two weeks of the experiment, and then slowly decreases and disappears over time of next eight weeks. As a result, the glycemia of control and VCO animals do not differ in last six weeks of the experiment. The area under the curve (AUC) presenting glycemia during whole the experiment is significantly lower in VCO animals than in the controls. The hypoglycemic effect of coconut oil is obviously dose-dependent since the amount of food (and therefore the coconut oil) that the animals eat decreases over the time. The results of the oral glucose tolerance test show that the OGTT AUC of VCO animals is significantly lower than the controls, and same is true for the insulin tolerance test. Finally, glycemia and insulin concentration in serums sampled on the last day of the experiment do not differ between VCO and Control groups, so accordingly neither HOMA-IR I and 2 (Homeostatic model assessment of insulin resistance) nor QUIC.Kl ( Quantitative Insulin Sensitivity Check Index). In conclusion, our results show beneficial effects of long-term high-dose coconut oil supplementation on rat glucose homeostasis.",
publisher = "BIT Congress Inc. (BIT Group Global Ltd.)",
journal = "BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017",
title = "The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis",
pages = "167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3422"
}

Đurašević, S., Jasnić, N., Dakić, T., Jevđović, T., Lakić, I., Vujović, P., Đorđević, J., Mitić-Ćulafić, D., Nikolić, B., Grigorov, I., Bogojević, D., Pavlović, S., Prokić, M., Zaletel, I.,& Todorović, Z.. (2017). The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
BIT Congress Inc. (BIT Group Global Ltd.)., 167.
https://hdl.handle.net/21.15107/rcub_ibiss_3422

Đurašević S, Jasnić N, Dakić T, Jevđović T, Lakić I, Vujović P, Đorđević J, Mitić-Ćulafić D, Nikolić B, Grigorov I, Bogojević D, Pavlović S, Prokić M, Zaletel I, Todorović Z. The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017. 2017;:167.
https://hdl.handle.net/21.15107/rcub_ibiss_3422 .

Đurašević, Siniša, Jasnić, Nebojša, Dakić, Tamara, Jevđović, Tanja, Lakić, Iva, Vujović, Predrag, Đorđević, Jelena, Mitić-Ćulafić, Dragana, Nikolić, Biljana, Grigorov, Ilijana, Bogojević, Desanka, Pavlović, Slađan, Prokić, Marko, Zaletel, Ivan, Todorović, Zoran, "The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis" in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017 (2017):167,
https://hdl.handle.net/21.15107/rcub_ibiss_3422 .

TY  - CONF
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Lakić, Iva
AU  - Vujović, Predrag
AU  - Đorđević, Jelena
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Pavlović, Slađan
AU  - Prokić, Marko
AU  - Zaletel, Ivan
AU  - Todorović, Zoran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3423
AB  - We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat liver and serum lipid status. Animals were divided into two groups with 8 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. On the last day of the experiment animals were killed, and blood and liver tissue were collected. In serum we measured the levels of total cholesterol (TC), high-density lipoproteins (HDL), non-HDL lipoproteins, triglycerides (TG), aspartate aminotransferase (9\.ST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). We also measured both liver and serum levels of high mobility group protein B 1 (HMGB 1) and haptoglobin (HP), as ,.vell as the liver level of NF-KB p65/ p-NF-KB p65 transcription factor, together with the histopathology analysis on liver slices and liver Comet assay. The results show that coconut oil do not change serum TC and HDL, but reduces non-HDL and TG levels (10% and 50%, respectively) comparing to control. As a result, atherogenic index of serum (AI) is strongly reduced in VCO group versus control. As for the liver status, results show that coconut supplementation increases AST, ALT and ALP levels in VCO group (50%, 30% and 60%, respectively) comparing to control. This effect is caused by the accumulation of coconut oil fat in liver, as confirmed by the histopathology showing signs of mild nonalcoholic steatohepatitis in VCO group, followed with the increased %of DNA in comet tail. The liver inflammation in VCO group is further demonstrated with the liver HP, HMGBl and p-NF-KB p65 level increase, and increase in nuclear level ofNF­kB p65, but not accompanying serum HP and HMGBl increase. In conclusion, our results show that coconut oil supplementation, despite causing mild and localized steatohepatitis, also lowers serum atherogcnic index, a predictor of cardiovascular risk.
PB  - BIT Congress Inc. (BIT Group Global Ltd.)
C3  - BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
T1  - The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3423
ER  - 

@conference{
author = "Đurašević, Siniša and Jasnić, Nebojša and Dakić, Tamara and Jevđović, Tanja and Lakić, Iva and Vujović, Predrag and Đorđević, Jelena and Mitić-Ćulafić, Dragana and Nikolić, Biljana and Grigorov, Ilijana and Bogojević, Desanka and Pavlović, Slađan and Prokić, Marko and Zaletel, Ivan and Todorović, Zoran",
year = "2017",
abstract = "We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat liver and serum lipid status. Animals were divided into two groups with 8 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. On the last day of the experiment animals were killed, and blood and liver tissue were collected. In serum we measured the levels of total cholesterol (TC), high-density lipoproteins (HDL), non-HDL lipoproteins, triglycerides (TG), aspartate aminotransferase (9\.ST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). We also measured both liver and serum levels of high mobility group protein B 1 (HMGB 1) and haptoglobin (HP), as ,.vell as the liver level of NF-KB p65/ p-NF-KB p65 transcription factor, together with the histopathology analysis on liver slices and liver Comet assay. The results show that coconut oil do not change serum TC and HDL, but reduces non-HDL and TG levels (10% and 50%, respectively) comparing to control. As a result, atherogenic index of serum (AI) is strongly reduced in VCO group versus control. As for the liver status, results show that coconut supplementation increases AST, ALT and ALP levels in VCO group (50%, 30% and 60%, respectively) comparing to control. This effect is caused by the accumulation of coconut oil fat in liver, as confirmed by the histopathology showing signs of mild nonalcoholic steatohepatitis in VCO group, followed with the increased %of DNA in comet tail. The liver inflammation in VCO group is further demonstrated with the liver HP, HMGBl and p-NF-KB p65 level increase, and increase in nuclear level ofNF­kB p65, but not accompanying serum HP and HMGBl increase. In conclusion, our results show that coconut oil supplementation, despite causing mild and localized steatohepatitis, also lowers serum atherogcnic index, a predictor of cardiovascular risk.",
publisher = "BIT Congress Inc. (BIT Group Global Ltd.)",
journal = "BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017",
title = "The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3423"
}

Đurašević, S., Jasnić, N., Dakić, T., Jevđović, T., Lakić, I., Vujović, P., Đorđević, J., Mitić-Ćulafić, D., Nikolić, B., Grigorov, I., Bogojević, D., Pavlović, S., Prokić, M., Zaletel, I.,& Todorović, Z.. (2017). The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
BIT Congress Inc. (BIT Group Global Ltd.)., 168.
https://hdl.handle.net/21.15107/rcub_ibiss_3423

Đurašević S, Jasnić N, Dakić T, Jevđović T, Lakić I, Vujović P, Đorđević J, Mitić-Ćulafić D, Nikolić B, Grigorov I, Bogojević D, Pavlović S, Prokić M, Zaletel I, Todorović Z. The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017. 2017;:168.
https://hdl.handle.net/21.15107/rcub_ibiss_3423 .

Đurašević, Siniša, Jasnić, Nebojša, Dakić, Tamara, Jevđović, Tanja, Lakić, Iva, Vujović, Predrag, Đorđević, Jelena, Mitić-Ćulafić, Dragana, Nikolić, Biljana, Grigorov, Ilijana, Bogojević, Desanka, Pavlović, Slađan, Prokić, Marko, Zaletel, Ivan, Todorović, Zoran, "The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids" in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017 (2017):168,
https://hdl.handle.net/21.15107/rcub_ibiss_3423 .