TY  - CONF
AU  - Škoro, Nikola
AU  - Jovanović, Olivera
AU  - Kumar, Amit
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Morina, Arian
AU  - Puač, Nevena
PY  - 2023
UR  - https://meetings.aps.org/Meeting/GEC23/Session/FF2.5
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6427
AB  - Non-thermal Plasmas (NTP), formed at atmospheric pressure, produce rich gas phase chemistry of Reactive Oxygen and Nitrogen Species (RONS). In a contact with a liquid target, NTPs induce production of different long-lived RONS species inside the liquid. NTPs can be employed for creation of plasma activated water (PAW) or for destruction of pollutants dissolved in water. In recent years both aspects are being developed as parts of a new field of plasma agricultural applications. The way to tailor the RONS concentrations is by adjusting of the plasma chemistry in the gas phase, the type of water and sample vessel properties. We will present results of plasma treatments of distilled and tap water using an atmospheric pressure plasma jet with pin electrode operating with addition of noble gas (He, Ar). The idea was to feature comparison of PAW properties obtained with different plasma system parameters. Produced PAW was used for treatments of different seeds and plants with an attention on linking the plasma properties with effects on treated plant material. On the other hand, the multi-pin jet was used for decontamination of water polluted by organic dyes and pharmaceuticals. This plasma source was designed based on a single-pin unit and obtained results can provide an insight into scaling up the plasma reactivity which is a necessary step for any agricultural application. We also tested variation in the efficiency of the plasma decontamination by adding recirculation of the contaminated sample. The effective treatment surface parameter proved to be significant for regulation of the decontamination level.
PB  - American Physical Society
C3  - The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA
T1  - Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6427
ER  - 

@conference{
author = "Škoro, Nikola and Jovanović, Olivera and Kumar, Amit and Živković, Suzana and Milutinović, Milica and Morina, Arian and Puač, Nevena",
year = "2023",
abstract = "Non-thermal Plasmas (NTP), formed at atmospheric pressure, produce rich gas phase chemistry of Reactive Oxygen and Nitrogen Species (RONS). In a contact with a liquid target, NTPs induce production of different long-lived RONS species inside the liquid. NTPs can be employed for creation of plasma activated water (PAW) or for destruction of pollutants dissolved in water. In recent years both aspects are being developed as parts of a new field of plasma agricultural applications. The way to tailor the RONS concentrations is by adjusting of the plasma chemistry in the gas phase, the type of water and sample vessel properties. We will present results of plasma treatments of distilled and tap water using an atmospheric pressure plasma jet with pin electrode operating with addition of noble gas (He, Ar). The idea was to feature comparison of PAW properties obtained with different plasma system parameters. Produced PAW was used for treatments of different seeds and plants with an attention on linking the plasma properties with effects on treated plant material. On the other hand, the multi-pin jet was used for decontamination of water polluted by organic dyes and pharmaceuticals. This plasma source was designed based on a single-pin unit and obtained results can provide an insight into scaling up the plasma reactivity which is a necessary step for any agricultural application. We also tested variation in the efficiency of the plasma decontamination by adding recirculation of the contaminated sample. The effective treatment surface parameter proved to be significant for regulation of the decontamination level.",
publisher = "American Physical Society",
journal = "The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA",
title = "Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6427"
}

Škoro, N., Jovanović, O., Kumar, A., Živković, S., Milutinović, M., Morina, A.,& Puač, N.. (2023). Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications. in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA
American Physical Society..
https://hdl.handle.net/21.15107/rcub_ibiss_6427

Škoro N, Jovanović O, Kumar A, Živković S, Milutinović M, Morina A, Puač N. Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications. in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6427 .

Škoro, Nikola, Jovanović, Olivera, Kumar, Amit, Živković, Suzana, Milutinović, Milica, Morina, Arian, Puač, Nevena, "Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications" in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6427 .

TY  - CONF
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Mišić, Danijela
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Jovanović, Olivera
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2023
UR  - https://europlantbiology2023.org/wp-content/uploads/2023/07/PBE2023-Abstract-Book.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6224
AB  - Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6224
ER  - 

@conference{
author = "Živković, Suzana and Milutinović, Milica and Mišić, Danijela and Nakarada, Đura and Mojović, Miloš and Jovanović, Olivera and Škoro, Nikola and Puač, Nevena",
year = "2023",
abstract = "Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6224"
}

Živković, S., Milutinović, M., Mišić, D., Nakarada, Đ., Mojović, M., Jovanović, O., Škoro, N.,& Puač, N.. (2023). Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224

Živković S, Milutinović M, Mišić D, Nakarada Đ, Mojović M, Jovanović O, Škoro N, Puač N. Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

Živković, Suzana, Milutinović, Milica, Mišić, Danijela, Nakarada, Đura, Mojović, Miloš, Jovanović, Olivera, Škoro, Nikola, Puač, Nevena, "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):154,
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

TY  - JOUR
AU  - Kumar, Amit
AU  - Škoro, Nikola
AU  - Gernjak, Wolfgang
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Živković, Suzana
AU  - Lumbaque, Elisabeth Cuervo
AU  - Farré, Maria José
AU  - Puač, Nevena
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0048969722082985
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5355
AB  - In this study, cold atmospheric plasma (CAP) was explored as a novel advanced oxidation process (AOP) for water decontamination. Samples with high concentration aqueous solutions of Diclofenac sodium (DCF) and 4-Chlorobenzoic acid (pCBA) were treated by plasma systems. Atmospheric pressure plasma jets (APPJs) with a 1 pin-electrode and multi-needle electrodes (3 pins) configurations were used. The plasma generated using argon as working gas was touching a stationary liquid surface in the case of pin electrode-APPJ while for multi-needle electrodes-APPJ the liquid sample was flowing during treatment. In both configurations, a commercial RF power supply was used for plasma ignition. Measurement of electrical signals enabled precise determination of power delivered from the plasma to the sample. The optical emission spectroscopy (OES) of plasma confirmed the appearance of excited reactive species in the plasma, such as hydroxyl radicals and atomic oxygen which are considered to be key reactive species in AOPs for the degradation of organic pollutants. Treatments were conducted with two different volumes (5 mL and 250 mL) of contaminated water samples. The data acquired allowed calculation of degradation efficiency and energy yield for both plasma sources. When treated with pin-APPJ, almost complete degradation of 5 mL DCF occurred in 1 min with the initial concentration of 25 mg/L and 50 mg/L, whereas 5 mL pCBA almost degraded in 10 min at the initial concentration of 25 mg/L and 40 mg/L. The treatment results with multi-needle electrodes system confirmed that DCF almost completely degraded in 30 min and pCBA degraded about 24 % in 50 min. The maximum calculated energy yield for 50 % removal was 6465 mg/kWh after treatment of 250 mL of DCF aqueous solution utilizing the plasma recirculation technique. The measurements also provided an insight to the kinetics of DCF and pCBA degradation. Degradation products and pathways for DCF were determined using LC-MS measurements.
PB  - Elsevier B.V.
T2  - Science of The Total Environment
T1  - Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source
VL  - 864
DO  - 10.1016/j.scitotenv.2022.161194
SP  - 161194
ER  - 

@article{
author = "Kumar, Amit and Škoro, Nikola and Gernjak, Wolfgang and Jovanović, Olivera and Petrović, Anđelija and Živković, Suzana and Lumbaque, Elisabeth Cuervo and Farré, Maria José and Puač, Nevena",
year = "2023",
abstract = "In this study, cold atmospheric plasma (CAP) was explored as a novel advanced oxidation process (AOP) for water decontamination. Samples with high concentration aqueous solutions of Diclofenac sodium (DCF) and 4-Chlorobenzoic acid (pCBA) were treated by plasma systems. Atmospheric pressure plasma jets (APPJs) with a 1 pin-electrode and multi-needle electrodes (3 pins) configurations were used. The plasma generated using argon as working gas was touching a stationary liquid surface in the case of pin electrode-APPJ while for multi-needle electrodes-APPJ the liquid sample was flowing during treatment. In both configurations, a commercial RF power supply was used for plasma ignition. Measurement of electrical signals enabled precise determination of power delivered from the plasma to the sample. The optical emission spectroscopy (OES) of plasma confirmed the appearance of excited reactive species in the plasma, such as hydroxyl radicals and atomic oxygen which are considered to be key reactive species in AOPs for the degradation of organic pollutants. Treatments were conducted with two different volumes (5 mL and 250 mL) of contaminated water samples. The data acquired allowed calculation of degradation efficiency and energy yield for both plasma sources. When treated with pin-APPJ, almost complete degradation of 5 mL DCF occurred in 1 min with the initial concentration of 25 mg/L and 50 mg/L, whereas 5 mL pCBA almost degraded in 10 min at the initial concentration of 25 mg/L and 40 mg/L. The treatment results with multi-needle electrodes system confirmed that DCF almost completely degraded in 30 min and pCBA degraded about 24 % in 50 min. The maximum calculated energy yield for 50 % removal was 6465 mg/kWh after treatment of 250 mL of DCF aqueous solution utilizing the plasma recirculation technique. The measurements also provided an insight to the kinetics of DCF and pCBA degradation. Degradation products and pathways for DCF were determined using LC-MS measurements.",
publisher = "Elsevier B.V.",
journal = "Science of The Total Environment",
title = "Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source",
volume = "864",
doi = "10.1016/j.scitotenv.2022.161194",
pages = "161194"
}

Kumar, A., Škoro, N., Gernjak, W., Jovanović, O., Petrović, A., Živković, S., Lumbaque, E. C., Farré, M. J.,& Puač, N.. (2023). Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source. in Science of The Total Environment
Elsevier B.V.., 864, 161194.
https://doi.org/10.1016/j.scitotenv.2022.161194

Kumar A, Škoro N, Gernjak W, Jovanović O, Petrović A, Živković S, Lumbaque EC, Farré MJ, Puač N. Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source. in Science of The Total Environment. 2023;864:161194.
doi:10.1016/j.scitotenv.2022.161194 .

Kumar, Amit, Škoro, Nikola, Gernjak, Wolfgang, Jovanović, Olivera, Petrović, Anđelija, Živković, Suzana, Lumbaque, Elisabeth Cuervo, Farré, Maria José, Puač, Nevena, "Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source" in Science of The Total Environment, 864 (2023):161194,
https://doi.org/10.1016/j.scitotenv.2022.161194 . .

TY  - CONF
AU  - Puač, Nevena
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - https://meetings.aps.org/Meeting/GEC22/Session/EF3.1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6323
AB  - The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.
PB  - American Physical Society
C3  - The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
T1  - Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells
SP  - EF3.00001
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6323
ER  - 

@conference{
author = "Puač, Nevena and Jovanović, Olivera and Petrović, Anđelija and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.",
publisher = "American Physical Society",
journal = "The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan",
title = "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells",
pages = "EF3.00001",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6323"
}

Puač, N., Jovanović, O., Petrović, A., Živković, S., Milutinović, M., Malović, G.,& Škoro, N.. (2022). Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
American Physical Society., EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323

Puač N, Jovanović O, Petrović A, Živković S, Milutinović M, Malović G, Škoro N. Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan. 2022;:EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

Puač, Nevena, Jovanović, Olivera, Petrović, Anđelija, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Škoro, Nikola, "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells" in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan (2022):EF3.00001,
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

TY  - JOUR
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4938
AB  - Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.
PB  - Basel: MDPI
T2  - Plants
T1  - Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma
IS  - 7
VL  - 11
DO  - 10.3390/plants11070907
SP  - 907
ER  - 

@article{
author = "Škoro, Nikola and Živković, Suzana and Jevremović, Slađana and Puač, Nevena",
year = "2022",
abstract = "Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma",
number = "7",
volume = "11",
doi = "10.3390/plants11070907",
pages = "907"
}

Škoro, N., Živković, S., Jevremović, S.,& Puač, N.. (2022). Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants
Basel: MDPI., 11(7), 907.
https://doi.org/10.3390/plants11070907

Škoro N, Živković S, Jevremović S, Puač N. Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants. 2022;11(7):907.
doi:10.3390/plants11070907 .

Škoro, Nikola, Živković, Suzana, Jevremović, Slađana, Puač, Nevena, "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma" in Plants, 11, no. 7 (2022):907,
https://doi.org/10.3390/plants11070907 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Zoran Lj.
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5268
AB  - The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5268
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Petrović, Zoran Lj. and Škoro, Nikola and Puač, Nevena",
year = "2022",
abstract = "The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5268"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Petrović, Z. Lj., Škoro, N.,& Puač, N.. (2022). Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268

Živković S, Jevremović S, Gašić U, Milutinović M, Petrović ZL, Škoro N, Puač N. Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Petrović, Zoran Lj., Škoro, Nikola, Puač, Nevena, "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):137,
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

TY  - CONF
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Devrnja, Nina
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Puač, Nevena
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5269
AB  - Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Molecular response to PAW in model plant species
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5269
ER  - 

@conference{
author = "Milutinović, Milica and Jovanović, Olivera and Devrnja, Nina and Todorović, Miloš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Puač, Nevena and Škoro, Nikola",
year = "2022",
abstract = "Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Molecular response to PAW in model plant species",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5269"
}

Milutinović, M., Jovanović, O., Devrnja, N., Todorović, M., Živković, S., Savić, J., Skorić, M., Puač, N.,& Škoro, N.. (2022). Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269

Milutinović M, Jovanović O, Devrnja N, Todorović M, Živković S, Savić J, Skorić M, Puač N, Škoro N. Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

Milutinović, Milica, Jovanović, Olivera, Devrnja, Nina, Todorović, Miloš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Puač, Nevena, Škoro, Nikola, "Molecular response to PAW in model plant species" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

TY  - CONF
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5271
AB  - The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.
C3  - Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands
T1  - Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5271
ER  - 

@conference{
author = "Puač, Nevena and Živković, Suzana and Milutinović, Milica and Jovanović, Olivera and Petrović, Anđelija and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.",
journal = "Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands",
title = "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions",
pages = "16",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5271"
}

Puač, N., Živković, S., Milutinović, M., Jovanović, O., Petrović, A., Malović, G.,& Škoro, N.. (2022). Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands, 16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271

Puač N, Živković S, Milutinović M, Jovanović O, Petrović A, Malović G, Škoro N. Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands. 2022;:16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

Puač, Nevena, Živković, Suzana, Milutinović, Milica, Jovanović, Olivera, Petrović, Anđelija, Malović, Gordana, Škoro, Nikola, "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions" in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands (2022):16,
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jevremović, Slađana
AU  - Petrović, Zoran Lj.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5415
AB  - In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins
PB  - Greifswald: Leibniz Institute for Plasma Science and Technology
C3  - Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
T1  - Changing the plant tissue morphology and physiology by plasma treatment
SP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5415
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Živković, Suzana and Milutinović, Milica and Jevremović, Slađana and Petrović, Zoran Lj.",
year = "2021",
abstract = "In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins",
publisher = "Greifswald: Leibniz Institute for Plasma Science and Technology",
journal = "Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany",
title = "Changing the plant tissue morphology and physiology by plasma treatment",
pages = "14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5415"
}

Puač, N., Škoro, N., Živković, S., Milutinović, M., Jevremović, S.,& Petrović, Z. Lj.. (2021). Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
Greifswald: Leibniz Institute for Plasma Science and Technology., 14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415

Puač N, Škoro N, Živković S, Milutinović M, Jevremović S, Petrović ZL. Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany. 2021;:14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

Puač, Nevena, Škoro, Nikola, Živković, Suzana, Milutinović, Milica, Jevremović, Slađana, Petrović, Zoran Lj., "Changing the plant tissue morphology and physiology by plasma treatment" in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany (2021):14,
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Petrović, Zoran Lj
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4414
AB  - Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.
PB  - National Institute for Lasers, Plasma and Radiation Physics
PB  - Alexandru Ioan Cuza University
PB  - Romanian Physical Society
C3  - 19th International conference on plasma physics and applications, CPPA 2021
T1  - Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4414
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Puač, Nevena and Škoro, Nikola and Petrović, Zoran Lj",
year = "2021",
abstract = "Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.",
publisher = "National Institute for Lasers, Plasma and Radiation Physics, Alexandru Ioan Cuza University, Romanian Physical Society",
journal = "19th International conference on plasma physics and applications, CPPA 2021",
title = "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4414"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Puač, N., Škoro, N.,& Petrović, Z. L.. (2021). Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021
National Institute for Lasers, Plasma and Radiation Physics., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414

Živković S, Jevremović S, Gašić U, Milutinović M, Puač N, Škoro N, Petrović ZL. Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021. 2021;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Puač, Nevena, Škoro, Nikola, Petrović, Zoran Lj, "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli" in 19th International conference on plasma physics and applications, CPPA 2021 (2021):130,
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Maletić, Dejan
AU  - Živković, Suzana
AU  - Selaković, Nenad
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2019
UR  - http://neon.dpp.fmph.uniba.sk/sappxxii/download/SAPP_XXII_JSPP_XI_Book_of_Contributed_Papers.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6199
AB  - Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.
PB  - Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava
C3  - Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
T1  - Diagnostics of atmospheric pressure plasmas and their application in agriculture
SP  - 68
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6199
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Maletić, Dejan and Živković, Suzana and Selaković, Nenad and Malović, Gordana and Petrović, Zoran Lj",
year = "2019",
abstract = "Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.",
publisher = "Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava",
journal = "Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia",
title = "Diagnostics of atmospheric pressure plasmas and their application in agriculture",
pages = "68-69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6199"
}

Puač, N., Škoro, N., Maletić, D., Živković, S., Selaković, N., Malović, G.,& Petrović, Z. L.. (2019). Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava., 68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199

Puač N, Škoro N, Maletić D, Živković S, Selaković N, Malović G, Petrović ZL. Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia. 2019;:68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .

Puač, Nevena, Škoro, Nikola, Maletić, Dejan, Živković, Suzana, Selaković, Nenad, Malović, Gordana, Petrović, Zoran Lj, "Diagnostics of atmospheric pressure plasmas and their application in agriculture" in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia (2019):68-69,
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Petrović, Zoran Lj
AU  - Škoro, Nikola
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4398
AB  - The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.
PB  - Heron Press
C3  - Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
T1  - Alteration in plant tissue morphology and physiology induced by plasma treatment
SP  - 63
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4398
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Milutinović, Milica and Puač, Nevena and Petrović, Zoran Lj and Škoro, Nikola",
year = "2019",
abstract = "The expansion of plasma medicine and its demand for precise and localized in vivo treatments of living cells and tissues resulted in the fast development of various plasma devices that operate at atmospheric pressure. In the present study, plant undifferentiated compact tissue (calli) of Balkan endemic small bearded iris (Iris reichenbachii Heuff.) was treated using a plasma needle device designed for biomedical applications and tested with numerous diagnostic procedures [1]. Significant morphological alterations of the iris calli were observed after direct plasma treatment. The indicated changes evaluated at the surface of the calli tissue after prolonged culture could be attributed to an enhanced cell division (mitotic activity) of the plant cells and differentiation of friable calli stimulated by low-temperature plasma. The current morphological changes were followed by the significant shift in calli physiology. The results showed that the plasma treatment enhanced the accumulation of specific arabinogalactan proteins in the epidermal cells and extracellular space of the iris calli. At the same time, the plant antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), that represent the main lines of plant cell defence against oxidative stress, exhibited different pattern of expression depending on the plasma treatment. The present study outlines that the plasma technique could be applied as an alternative and valuable approach for promoting regeneration and multiplication in a plant meristematic tissue culture.",
publisher = "Heron Press",
journal = "Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)",
title = "Alteration in plant tissue morphology and physiology induced by plasma treatment",
pages = "63-64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4398"
}

Živković, S., Jevremović, S., Milutinović, M., Puač, N., Petrović, Z. L.,& Škoro, N.. (2019). Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019)
Heron Press., 63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398

Živković S, Jevremović S, Milutinović M, Puač N, Petrović ZL, Škoro N. Alteration in plant tissue morphology and physiology induced by plasma treatment. in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019). 2019;:63-64.
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

Živković, Suzana, Jevremović, Slađana, Milutinović, Milica, Puač, Nevena, Petrović, Zoran Lj, Škoro, Nikola, "Alteration in plant tissue morphology and physiology induced by plasma treatment" in Program Abstracts of the Twenty-First International Summer School on Vacuum, Electron and Ion Technologies (VEIT 2019) (2019):63-64,
https://hdl.handle.net/21.15107/rcub_ibiss_4398 .

TY  - JOUR
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4395
AB  - In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low-pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment conditions.
PB  - Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA
T2  - Plasma Processes and Polymers
T1  - Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water
IS  - 2
VL  - 15
DO  - 10.1002/ppap.201700082
SP  - e1700082
ER  - 

@article{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Petrović, Zoran Lj",
year = "2018",
abstract = "In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low-pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment conditions.",
publisher = "Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA",
journal = "Plasma Processes and Polymers",
title = "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water",
number = "2",
volume = "15",
doi = "10.1002/ppap.201700082",
pages = "e1700082"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Malović, G.,& Petrović, Z. L.. (2018). Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers
Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA., 15(2), e1700082.
https://doi.org/10.1002/ppap.201700082

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Malović G, Petrović ZL. Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers. 2018;15(2):e1700082.
doi:10.1002/ppap.201700082 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Petrović, Zoran Lj, "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water" in Plasma Processes and Polymers, 15, no. 2 (2018):e1700082,
https://doi.org/10.1002/ppap.201700082 . .

TY  - JOUR
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2018
UR  - http://doi.wiley.com/10.1002/ppap.201700082
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3538
AB  - In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low‐pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment
T2  - Plasma Processes and Polymers
T1  - Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water
IS  - 2
VL  - 15
DO  - 10.1002/ppap.201700082
SP  - 1700082
ER  - 

@article{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Petrović, Zoran Lj.",
year = "2018",
abstract = "In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low‐pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment",
journal = "Plasma Processes and Polymers",
title = "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water",
number = "2",
volume = "15",
doi = "10.1002/ppap.201700082",
pages = "1700082"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Malović, G.,& Petrović, Z. Lj.. (2018). Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers, 15(2), 1700082.
https://doi.org/10.1002/ppap.201700082

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Malović G, Petrović ZL. Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers. 2018;15(2):1700082.
doi:10.1002/ppap.201700082 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Petrović, Zoran Lj., "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water" in Plasma Processes and Polymers, 15, no. 2 (2018):1700082,
https://doi.org/10.1002/ppap.201700082 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Petrović, Zoran Lj
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4397
AB  - Plant callus of three different and phylogenetically distinct types of plants-carrot (Daucus carrota L.), and two ornamental plant species: horned pansy (Viola cornuta L. ‛Lutea Splendens’) and small bearded iris (Iris reichenbachii Heuff.) was treated using plasma needle. Plasma treatment induced somatic embryogenesis in carrot calli cultured on basal medium and stimulated growth of both carrot calli and transgenic calli of horned pansy. Surface changes of nonregenerative globular calli of iris after plasma treatment were followed by enhanced accumulation of arabinogalactan proteins in epidermal cells and around the globule. Our findings indicate that the plasma treatment could be considered as useful and promising technique for induction of embryogenesis, as well as for regeneration and multiplication of transgenic plants.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications
T1  - Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues
IS  - WS2-5
VL  - 2
SP  - 646
EP  - 649
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4397
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Puač, Nevena and Škoro, Nikola and Petrović, Zoran Lj",
year = "2018",
abstract = "Plant callus of three different and phylogenetically distinct types of plants-carrot (Daucus carrota L.), and two ornamental plant species: horned pansy (Viola cornuta L. ‛Lutea Splendens’) and small bearded iris (Iris reichenbachii Heuff.) was treated using plasma needle. Plasma treatment induced somatic embryogenesis in carrot calli cultured on basal medium and stimulated growth of both carrot calli and transgenic calli of horned pansy. Surface changes of nonregenerative globular calli of iris after plasma treatment were followed by enhanced accumulation of arabinogalactan proteins in epidermal cells and around the globule. Our findings indicate that the plasma treatment could be considered as useful and promising technique for induction of embryogenesis, as well as for regeneration and multiplication of transgenic plants.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications",
title = "Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues",
number = "WS2-5",
volume = "2",
pages = "646-649",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4397"
}

Živković, S., Jevremović, S., Puač, N., Škoro, N.,& Petrović, Z. L.. (2018). Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues. in Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications
Belgrade: Serbian Academy of Sciences and Arts., 2(WS2-5), 646-649.
https://hdl.handle.net/21.15107/rcub_ibiss_4397

Živković S, Jevremović S, Puač N, Škoro N, Petrović ZL. Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues. in Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications. 2018;2(WS2-5):646-649.
https://hdl.handle.net/21.15107/rcub_ibiss_4397 .

Živković, Suzana, Jevremović, Slađana, Puač, Nevena, Škoro, Nikola, Petrović, Zoran Lj, "Morphological and physiological aspects of plasma treatment-induced changes in plant cells and tissues" in Invited Progress Reports, Advanced Plasma Technologies in medicine, biotechnology and agriculture. Proceedings of the 22nd International Conference on Gas Discharges and their applications, 2, no. WS2-5 (2018):646-649,
https://hdl.handle.net/21.15107/rcub_ibiss_4397 .

TY  - JOUR
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Krstić Milošević, Dijana
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2018
UR  - http://link.springer.com/10.1140/epjd/e2017-80329-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3030
AB  - Today’s reality is connected with mitigation of threats from the new chemical and biological warfare agents. A novel investigation of cold plasmas in contact with liquids presented in this paper demonstrated that the chemically reactive environment produced by atmospheric pressure plasma jet (APPJ) is potentially capable of rapid destruction of chemical warfare agents in a broad spectrum. The decontamination of three different chemical warfare agent surrogates dissolved in liquid is investigated by using an easily transportable APPJ. The jet is powered by a kHz signal source connected to a low-voltage DC source and with He as working gas. The detailed investigation of electrical properties is performed for various plasmas at different distances from the sample. The measurements of plasma properties in situ are supported by the optical spectrometry measurements, whereas the high performance liquid chromatography measurements before and after the treatment of aqueous solutions of Malathion, Fenitrothion and Dimethyl Methylphosphonate. These solutions are used to evaluate destruction and its efficiency for specific neural agent simulants. The particular removal rates are found to be from 56% up to 96% during 10 min treatment. The data obtained provide basis to evaluate APPJ’s efficiency at different operating conditions. The presented results are promising and could be improved with different operating conditions and optimization of the decontamination process.
T2  - The European Physical Journal D
T1  - Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet
IS  - 1
VL  - 72
DO  - 10.1140/epjd/e2017-80329-9
SP  - 2
ER  - 

@article{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Krstić Milošević, Dijana and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran Lj.",
year = "2018",
abstract = "Today’s reality is connected with mitigation of threats from the new chemical and biological warfare agents. A novel investigation of cold plasmas in contact with liquids presented in this paper demonstrated that the chemically reactive environment produced by atmospheric pressure plasma jet (APPJ) is potentially capable of rapid destruction of chemical warfare agents in a broad spectrum. The decontamination of three different chemical warfare agent surrogates dissolved in liquid is investigated by using an easily transportable APPJ. The jet is powered by a kHz signal source connected to a low-voltage DC source and with He as working gas. The detailed investigation of electrical properties is performed for various plasmas at different distances from the sample. The measurements of plasma properties in situ are supported by the optical spectrometry measurements, whereas the high performance liquid chromatography measurements before and after the treatment of aqueous solutions of Malathion, Fenitrothion and Dimethyl Methylphosphonate. These solutions are used to evaluate destruction and its efficiency for specific neural agent simulants. The particular removal rates are found to be from 56% up to 96% during 10 min treatment. The data obtained provide basis to evaluate APPJ’s efficiency at different operating conditions. The presented results are promising and could be improved with different operating conditions and optimization of the decontamination process.",
journal = "The European Physical Journal D",
title = "Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet",
number = "1",
volume = "72",
doi = "10.1140/epjd/e2017-80329-9",
pages = "2"
}

Škoro, N., Puač, N., Živković, S., Krstić Milošević, D., Cvelbar, U., Malović, G.,& Petrović, Z. Lj.. (2018). Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet. in The European Physical Journal D, 72(1), 2.
https://doi.org/10.1140/epjd/e2017-80329-9

Škoro N, Puač N, Živković S, Krstić Milošević D, Cvelbar U, Malović G, Petrović ZL. Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet. in The European Physical Journal D. 2018;72(1):2.
doi:10.1140/epjd/e2017-80329-9 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Krstić Milošević, Dijana, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran Lj., "Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet" in The European Physical Journal D, 72, no. 1 (2018):2,
https://doi.org/10.1140/epjd/e2017-80329-9 . .

TY  - CONF
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6222
AB  - Non-equilibrium atmospheric pressure plasmas (APP) are chemically active media with properties adjustable to comply with various application demands. ln biomedical field, so far APP have demonstrated excellent applicability in sterilization and cleaning, i.e. for treatment of organic materials and microorganisms. Atmospheric pressure plasma jets (APPJ) proved to be technically simple and efficient sources of cold APP which are suitable for different application setups. Recently, a new research field-plasmas in agriculture emerged, widening number of applications. Since APPJs can also operate in contact with liquids, they are suitable for new applications in plasma agriculture. Initial investigations show that chemically reactive environment produced by these sources can influence and modify physical and chemical properties of liquids. As one of the main surface water contaminants are pesticides used in agriculture our idea is to use APP for water detoxification. Namely, we conducted a study on decontamination of water samples polluted with different pesticides, i.e. organophosphate compounds, by using APPJs. We used two different jet configurations powered by a kHz signal source with He as working gas and treated liquid samples at different distances between the jet and the sample surface, for different duration times and for different liquid sample volumes. Optical and electrical characterization of the APPJs is performed in order to obtain information about the plasma properties and stability of the treatment conditions. Liquid sample analysis before and after the treatment are executed by using high performance liquid chromatography (HPLC) and liquid chromatography coupled with mass spectrometry (LC-MS) in order to follow degradation of organophosphates. Significant and efficient degradation of both pesticides is noticed and appearance of degradation products is observed in the liquid sample. Dependence of the decontamination efficiency on treatment time is determined. We also investigated toxicity of degradation products. Thus, from the data obtained we could assess the decontamination efficiency for different APPJ types and also to provide information about mechanisms governing the plasma destruction of organophosphates.
PB  - Zagreb: Institute of Physics
C3  - Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia
T1  - Use of atmospheric pressure plasmas for decontamination of water containing organophosphates
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6222
ER  - 

@conference{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran",
year = "2017",
abstract = "Non-equilibrium atmospheric pressure plasmas (APP) are chemically active media with properties adjustable to comply with various application demands. ln biomedical field, so far APP have demonstrated excellent applicability in sterilization and cleaning, i.e. for treatment of organic materials and microorganisms. Atmospheric pressure plasma jets (APPJ) proved to be technically simple and efficient sources of cold APP which are suitable for different application setups. Recently, a new research field-plasmas in agriculture emerged, widening number of applications. Since APPJs can also operate in contact with liquids, they are suitable for new applications in plasma agriculture. Initial investigations show that chemically reactive environment produced by these sources can influence and modify physical and chemical properties of liquids. As one of the main surface water contaminants are pesticides used in agriculture our idea is to use APP for water detoxification. Namely, we conducted a study on decontamination of water samples polluted with different pesticides, i.e. organophosphate compounds, by using APPJs. We used two different jet configurations powered by a kHz signal source with He as working gas and treated liquid samples at different distances between the jet and the sample surface, for different duration times and for different liquid sample volumes. Optical and electrical characterization of the APPJs is performed in order to obtain information about the plasma properties and stability of the treatment conditions. Liquid sample analysis before and after the treatment are executed by using high performance liquid chromatography (HPLC) and liquid chromatography coupled with mass spectrometry (LC-MS) in order to follow degradation of organophosphates. Significant and efficient degradation of both pesticides is noticed and appearance of degradation products is observed in the liquid sample. Dependence of the decontamination efficiency on treatment time is determined. We also investigated toxicity of degradation products. Thus, from the data obtained we could assess the decontamination efficiency for different APPJ types and also to provide information about mechanisms governing the plasma destruction of organophosphates.",
publisher = "Zagreb: Institute of Physics",
journal = "Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia",
title = "Use of atmospheric pressure plasmas for decontamination of water containing organophosphates",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6222"
}

Škoro, N., Puač, N., Živković, S., Cvelbar, U., Malović, G.,& Petrović, Z.. (2017). Use of atmospheric pressure plasmas for decontamination of water containing organophosphates. in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia
Zagreb: Institute of Physics., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_6222

Škoro N, Puač N, Živković S, Cvelbar U, Malović G, Petrović Z. Use of atmospheric pressure plasmas for decontamination of water containing organophosphates. in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia. 2017;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_6222 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran, "Use of atmospheric pressure plasmas for decontamination of water containing organophosphates" in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia (2017):28,
https://hdl.handle.net/21.15107/rcub_ibiss_6222 .

TY  - CONF
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Mišić, Danijela
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6221
AB  - Nonequilibrium atmospheric pressure plasma proved to produce reactive chemistry environment at gas temperatures as low as room temperature which is perfect for treatments of different kind of surfaces. Over the past several years cold atmospheric pressure plasma applications in surface sterilization and cleaning have been extensively studied. Recently, the research has been directed to utilization of this type of plasma far agriculture. Plasma is used directly for treatment of seeds, but, also, indirectly through plasma activated water. In the case of decontamination of water contaminated with conventional pesticides and insecticides plasma can play important role. One of suitable plasma sources for these treatments are atmospheric pressure plasma jets (APPJ), since they are small-size (easily scalable to larger systems), relatively technically simple and cost-effective devices. In our case, we investigated treatments of water contaminated with three types of pesticides. Decontamination is achieved by applying the jet on the liquid surface generating high-energy electrons, reactive oxygen and nitrogen species, radicals and ultraviolet radiation. Reactive species created al the gas-liquid interface effectively degrade organic pollutants in the water. However, due to the complexity of the system, key mechanisms important for destruction of particular contaminant are not clearly known. Moreover, optimisation of the system is recondite by reciprocal influence of the jet and surface. Thus, we have performed detailed and systematic investigation of the APPJ-liquid sample system properties at different conditions and far different pollutants dissolved in water. Electrical and optical measurements provided information about the discharge running at different powers and distances from the sample. We could relate electrical characteristics with spatial profiles of the discharge. Moreover, optical spectrometry provided data on emission lines characteristic far the plasma used far decontamination. By using liquid chromatography measurements of contaminated water samples before and after the treatment we acquired evidence on destruction of specific pollutants and found decontamination rates. AII this data is valuable far evaluation of APPJ efficiency at different operating conditions and optimisation of the decontamination process.
PB  - lnstitute of Plasma Physics CAS
C3  - Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic
T1  - Application of atmospheric pressure plasmas in agriculture for wastewater cleaning
SP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6221
ER  - 

@conference{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Mišić, Danijela and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran",
year = "2017",
abstract = "Nonequilibrium atmospheric pressure plasma proved to produce reactive chemistry environment at gas temperatures as low as room temperature which is perfect for treatments of different kind of surfaces. Over the past several years cold atmospheric pressure plasma applications in surface sterilization and cleaning have been extensively studied. Recently, the research has been directed to utilization of this type of plasma far agriculture. Plasma is used directly for treatment of seeds, but, also, indirectly through plasma activated water. In the case of decontamination of water contaminated with conventional pesticides and insecticides plasma can play important role. One of suitable plasma sources for these treatments are atmospheric pressure plasma jets (APPJ), since they are small-size (easily scalable to larger systems), relatively technically simple and cost-effective devices. In our case, we investigated treatments of water contaminated with three types of pesticides. Decontamination is achieved by applying the jet on the liquid surface generating high-energy electrons, reactive oxygen and nitrogen species, radicals and ultraviolet radiation. Reactive species created al the gas-liquid interface effectively degrade organic pollutants in the water. However, due to the complexity of the system, key mechanisms important for destruction of particular contaminant are not clearly known. Moreover, optimisation of the system is recondite by reciprocal influence of the jet and surface. Thus, we have performed detailed and systematic investigation of the APPJ-liquid sample system properties at different conditions and far different pollutants dissolved in water. Electrical and optical measurements provided information about the discharge running at different powers and distances from the sample. We could relate electrical characteristics with spatial profiles of the discharge. Moreover, optical spectrometry provided data on emission lines characteristic far the plasma used far decontamination. By using liquid chromatography measurements of contaminated water samples before and after the treatment we acquired evidence on destruction of specific pollutants and found decontamination rates. AII this data is valuable far evaluation of APPJ efficiency at different operating conditions and optimisation of the decontamination process.",
publisher = "lnstitute of Plasma Physics CAS",
journal = "Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic",
title = "Application of atmospheric pressure plasmas in agriculture for wastewater cleaning",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6221"
}

Škoro, N., Puač, N., Živković, S., Mišić, D., Cvelbar, U., Malović, G.,& Petrović, Z.. (2017). Application of atmospheric pressure plasmas in agriculture for wastewater cleaning. in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic
lnstitute of Plasma Physics CAS., 13.
https://hdl.handle.net/21.15107/rcub_ibiss_6221

Škoro N, Puač N, Živković S, Mišić D, Cvelbar U, Malović G, Petrović Z. Application of atmospheric pressure plasmas in agriculture for wastewater cleaning. in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic. 2017;:13.
https://hdl.handle.net/21.15107/rcub_ibiss_6221 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Mišić, Danijela, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran, "Application of atmospheric pressure plasmas in agriculture for wastewater cleaning" in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic (2017):13,
https://hdl.handle.net/21.15107/rcub_ibiss_6221 .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Šašić, Vuk
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6198
AB  - In this abstract we report on influence of direct and indirect plasma treatments on catalase enzyme activity in Paulownia tomentosa seeds. The direct treatment of the seeds was performed in low-pressure RF plasma system for different treatment times. After treatments these seeds were imbibed with distilled water. The other set of P. tomentosa seeds was imbibed with plasma activated water (PAW). PAW was produced by using atmospheric pressure plasma source in treatments with different durations. Seeds from both sets were exposed to the same conditions and after 5 days activity of catalase enzyme was measured. In comparison to the control sample, differences in the activity was observed both regarding direct and PAW treated seeds and regarding duration of treatments.
PB  - Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa
C3  - Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal
T1  - Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6198
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Šašić, Vuk and Malović, Gordana and Petrović, Zoran Lj",
year = "2017",
abstract = "In this abstract we report on influence of direct and indirect plasma treatments on catalase enzyme activity in Paulownia tomentosa seeds. The direct treatment of the seeds was performed in low-pressure RF plasma system for different treatment times. After treatments these seeds were imbibed with distilled water. The other set of P. tomentosa seeds was imbibed with plasma activated water (PAW). PAW was produced by using atmospheric pressure plasma source in treatments with different durations. Seeds from both sets were exposed to the same conditions and after 5 days activity of catalase enzyme was measured. In comparison to the control sample, differences in the activity was observed both regarding direct and PAW treated seeds and regarding duration of treatments.",
publisher = "Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa",
journal = "Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal",
title = "Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6198"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Šašić, V., Malović, G.,& Petrović, Z. L.. (2017). Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water. in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal
Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa., 180.
https://hdl.handle.net/21.15107/rcub_ibiss_6198

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Šašić V, Malović G, Petrović ZL. Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water. in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal. 2017;:180.
https://hdl.handle.net/21.15107/rcub_ibiss_6198 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Šašić, Vuk, Malović, Gordana, Petrović, Zoran Lj, "Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water" in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal (2017):180,
https://hdl.handle.net/21.15107/rcub_ibiss_6198 .